Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056693.t1 | XP_003606879.2 | 92.7 | 96 | 5 | 1 | 1 | 96 | 11 | 104 | 4.10E-40 | 173.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056693.t1 | G7JG36 | 92.7 | 96 | 5 | 1 | 1 | 96 | 1 | 94 | 3.0e-40 | 173.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049411 | MS.gene056693 | 0.828304 | 9.69E-55 | -1.69E-46 |
| MS.gene054004 | MS.gene056693 | 0.812307 | 4.53E-51 | -1.69E-46 |
| MS.gene055339 | MS.gene056693 | 0.826167 | 3.15E-54 | -1.69E-46 |
| MS.gene055765 | MS.gene056693 | 0.808848 | 2.53E-50 | -1.69E-46 |
| MS.gene056693 | MS.gene059802 | 0.844282 | 8.33E-59 | -1.69E-46 |
| MS.gene056693 | MS.gene063134 | 0.801091 | 1.06E-48 | -1.69E-46 |
| MS.gene056693 | MS.gene06541 | 0.819755 | 9.83E-53 | -1.69E-46 |
| MS.gene056693 | MS.gene068742 | 0.84309 | 1.73E-58 | -1.69E-46 |
| MS.gene056693 | MS.gene069828 | 0.826669 | 2.39E-54 | -1.69E-46 |
| MS.gene056693 | MS.gene073207 | 0.831733 | 1.41E-55 | -1.69E-46 |
| MS.gene056693 | MS.gene24531 | 0.801268 | 9.76E-49 | -1.69E-46 |
| MS.gene056693 | MS.gene28021 | -0.804014 | 2.65E-49 | -1.69E-46 |
| MS.gene056693 | MS.gene39835 | -0.813012 | 3.17E-51 | -1.69E-46 |
| MS.gene056693 | MS.gene45692 | 0.962892 | 2.02E-121 | -1.69E-46 |
| MS.gene056693 | MS.gene50071 | 0.99516 | 1.42E-213 | -1.69E-46 |
| MS.gene056693 | MS.gene54747 | 0.809691 | 1.67E-50 | -1.69E-46 |
| MS.gene056693 | MS.gene61153 | 0.814282 | 1.67E-51 | -1.69E-46 |
| MS.gene056693 | MS.gene61588 | 0.803212 | 3.88E-49 | -1.69E-46 |
| MS.gene056693 | MS.gene68213 | 0.822028 | 2.95E-53 | -1.69E-46 |
| MS.gene056693 | MS.gene68806 | 0.803166 | 3.97E-49 | -1.69E-46 |
| MS.gene056693 | MS.gene69820 | 0.829096 | 6.23E-55 | -1.69E-46 |
| MS.gene056693 | MS.gene71891 | -0.817369 | 3.42E-52 | -1.69E-46 |
| MS.gene056693 | MS.gene72950 | -0.801054 | 1.08E-48 | -1.69E-46 |
| MS.gene056693 | MS.gene75344 | 0.824779 | 6.71E-54 | -1.69E-46 |
| MS.gene056693 | MS.gene82421 | 0.813879 | 2.05E-51 | -1.69E-46 |
| MS.gene056693 | MS.gene83864 | 0.812496 | 4.12E-51 | -1.69E-46 |
| MS.gene056693 | MS.gene87292 | 0.809688 | 1.67E-50 | -1.69E-46 |
| MS.gene056693 | MS.gene89018 | 0.810207 | 1.29E-50 | -1.69E-46 |
| MS.gene056693 | MS.gene91203 | 0.827538 | 1.48E-54 | -1.69E-46 |
| MS.gene056693 | MS.gene92601 | 0.829199 | 5.88E-55 | -1.69E-46 |
| MS.gene056693 | MS.gene96211 | 0.830232 | 3.30E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056693.t1 | MTR_4g069770 | 92.708 | 96 | 5 | 1 | 1 | 96 | 1 | 94 | 2.11e-61 | 182 |
| MS.gene056693.t1 | MTR_5g095980 | 60.215 | 93 | 30 | 2 | 1 | 87 | 1 | 92 | 1.28e-25 | 91.7 |
| MS.gene056693.t1 | MTR_3g007880 | 61.538 | 78 | 24 | 1 | 1 | 72 | 1 | 78 | 3.09e-22 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056693.t1 | AT1G49310 | 42.254 | 71 | 37 | 2 | 8 | 74 | 12 | 82 | 7.23e-13 | 58.9 |
Find 11 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTTCTTCTTCTGCTTCT+TGG | 0.153872 | 4.3:+35738475 | None:intergenic |
| GAAAAGACTTGGGAGATTAT+TGG | 0.261061 | 4.3:-35738631 | MS.gene056693:CDS |
| ACAAGTTCTTTGATTGCTTC+AGG | 0.324229 | 4.3:+35738581 | None:intergenic |
| AACTCTGAACAAGAGCTTCA+TGG | 0.354626 | 4.3:-35738437 | MS.gene056693:CDS |
| CTCTTATGCAAGAAAAGACT+TGG | 0.442343 | 4.3:-35738642 | MS.gene056693:CDS |
| GAGTATGAGAGAAGCAAAGA+TGG | 0.451907 | 4.3:+35738826 | None:intergenic |
| TCTTTGATTGCTTCAGGCAT+AGG | 0.472850 | 4.3:+35738587 | None:intergenic |
| GTGTGATATAATATGACATT+AGG | 0.480243 | 4.3:+35738509 | None:intergenic |
| TGATTGCTTCAGGCATAGGT+TGG | 0.514568 | 4.3:+35738591 | None:intergenic |
| AGAACTTGTTCAAAATCAAG+AGG | 0.539159 | 4.3:-35738567 | MS.gene056693:CDS |
| TCTTATGCAAGAAAAGACTT+GGG | 0.611202 | 4.3:-35738641 | MS.gene056693:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATATAGTATAAATTGAT+TGG | + | chr4.3:35738556-35738575 | None:intergenic | 15.0% |
| !!! | AATTTTCTTTGTACTAAAAT+AGG | - | chr4.3:35738588-35738607 | MS.gene056693:CDS | 15.0% |
| !! | TTAGTACAAAGAAAATTGAA+AGG | + | chr4.3:35738585-35738604 | None:intergenic | 20.0% |
| ! | GTGTGATATAATATGACATT+AGG | + | chr4.3:35738757-35738776 | None:intergenic | 25.0% |
| TCTTATGCAAGAAAAGACTT+GGG | - | chr4.3:35738622-35738641 | MS.gene056693:CDS | 30.0% | |
| ! | AGAACTTGTTCAAAATCAAG+AGG | - | chr4.3:35738696-35738715 | MS.gene056693:intron | 30.0% |
| ACAAGTTCTTTGATTGCTTC+AGG | + | chr4.3:35738685-35738704 | None:intergenic | 35.0% | |
| CTCTTATGCAAGAAAAGACT+TGG | - | chr4.3:35738621-35738640 | MS.gene056693:CDS | 35.0% | |
| GAAAAGACTTGGGAGATTAT+TGG | - | chr4.3:35738632-35738651 | MS.gene056693:CDS | 35.0% | |
| ! | AGTCTTTTCTTGCATAAGAG+AGG | + | chr4.3:35738621-35738640 | None:intergenic | 35.0% |
| ! | CATACTCTTTTCTCTTCTTC+TGG | - | chr4.3:35738453-35738472 | MS.gene056693:CDS | 35.0% |
| ! | TTGTTTCTTCTTCTGCTTCT+TGG | + | chr4.3:35738791-35738810 | None:intergenic | 35.0% |
| AACTCTGAACAAGAGCTTCA+TGG | - | chr4.3:35738826-35738845 | MS.gene056693:CDS | 40.0% | |
| GAGTATGAGAGAAGCAAAGA+TGG | + | chr4.3:35738440-35738459 | None:intergenic | 40.0% | |
| TCTTTGATTGCTTCAGGCAT+AGG | + | chr4.3:35738679-35738698 | None:intergenic | 40.0% | |
| TGATTGCTTCAGGCATAGGT+TGG | + | chr4.3:35738675-35738694 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 35738430 | 35738855 | 35738430 | ID=MS.gene056693 |
| chr4.3 | mRNA | 35738430 | 35738855 | 35738430 | ID=MS.gene056693.t1;Parent=MS.gene056693 |
| chr4.3 | exon | 35738811 | 35738855 | 35738811 | ID=MS.gene056693.t1.exon1;Parent=MS.gene056693.t1 |
| chr4.3 | CDS | 35738811 | 35738855 | 35738811 | ID=cds.MS.gene056693.t1;Parent=MS.gene056693.t1 |
| chr4.3 | exon | 35738430 | 35738675 | 35738430 | ID=MS.gene056693.t1.exon2;Parent=MS.gene056693.t1 |
| chr4.3 | CDS | 35738430 | 35738675 | 35738430 | ID=cds.MS.gene056693.t1;Parent=MS.gene056693.t1 |
| Gene Sequence |
| Protein sequence |
