Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45710.t1 | XP_013456377.1 | 91.3 | 104 | 9 | 0 | 1 | 104 | 1 | 104 | 1.50E-48 | 201.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45710.t1 | Q8W453 | 41.6 | 77 | 42 | 1 | 28 | 104 | 29 | 102 | 3.2e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45710.t1 | I3SWL0 | 91.3 | 104 | 9 | 0 | 1 | 104 | 1 | 104 | 1.1e-48 | 201.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050710 | MS.gene45710 | 0.804791 | 1.82E-49 | -1.69E-46 |
| MS.gene051439 | MS.gene45710 | 0.823065 | 1.69E-53 | -1.69E-46 |
| MS.gene053108 | MS.gene45710 | 0.829721 | 4.39E-55 | -1.69E-46 |
| MS.gene055858 | MS.gene45710 | 0.802535 | 5.36E-49 | -1.69E-46 |
| MS.gene056618 | MS.gene45710 | 0.821641 | 3.63E-53 | -1.69E-46 |
| MS.gene056675 | MS.gene45710 | 0.821512 | 3.88E-53 | -1.69E-46 |
| MS.gene05926 | MS.gene45710 | 0.816893 | 4.37E-52 | -1.69E-46 |
| MS.gene05960 | MS.gene45710 | 0.862696 | 4.22E-64 | -1.69E-46 |
| MS.gene060405 | MS.gene45710 | 0.809533 | 1.81E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45710.t1 | MTR_4g069220 | 91.346 | 104 | 9 | 0 | 1 | 104 | 1 | 104 | 2.35e-68 | 200 |
| MS.gene45710.t1 | MTR_4g069170 | 73.077 | 104 | 28 | 0 | 1 | 104 | 1 | 104 | 1.43e-52 | 160 |
| MS.gene45710.t1 | MTR_4g069200 | 55.238 | 105 | 45 | 2 | 1 | 104 | 1 | 104 | 2.54e-37 | 122 |
| MS.gene45710.t1 | MTR_4g069210 | 42.857 | 105 | 52 | 2 | 1 | 105 | 1 | 97 | 2.67e-21 | 84.0 |
| MS.gene45710.t1 | MTR_7g052640 | 41.748 | 103 | 57 | 1 | 2 | 104 | 5 | 104 | 1.10e-20 | 79.7 |
| MS.gene45710.t1 | MTR_5g094210 | 33.654 | 104 | 60 | 4 | 2 | 104 | 7 | 102 | 2.33e-11 | 55.8 |
| MS.gene45710.t1 | MTR_3g055250 | 36.667 | 90 | 49 | 3 | 7 | 96 | 4 | 85 | 5.49e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45710.t1 | AT5G48490 | 36.634 | 101 | 60 | 2 | 5 | 104 | 4 | 101 | 3.89e-14 | 63.2 |
| MS.gene45710.t1 | AT5G48485 | 41.558 | 77 | 42 | 1 | 28 | 104 | 29 | 102 | 5.82e-14 | 62.8 |
Find 32 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGGGTTGCTTTCTTTCTA+TGG | 0.215569 | 4.4:-38336096 | MS.gene45710:CDS |
| CTATGTCGTTATAAGGATTC+AGG | 0.249484 | 4.4:-38336114 | MS.gene45710:CDS |
| TCCTGTTAACTGTAAGCTTA+TGG | 0.254280 | 4.4:-38336046 | MS.gene45710:CDS |
| TCAATGCCTATGTCGTTATA+AGG | 0.293667 | 4.4:-38336121 | MS.gene45710:CDS |
| AATACCATGATGTTGGTTAA+TGG | 0.374528 | 4.4:-38336270 | MS.gene45710:CDS |
| AGTGGTGACAATTCTGTTAA+TGG | 0.378651 | 4.4:-38336192 | MS.gene45710:CDS |
| TCCATAAGCTTACAGTTAAC+AGG | 0.382556 | 4.4:+38336045 | None:intergenic |
| GGAAGTTCCATGGCTTGGTT+AGG | 0.427875 | 4.4:+38336066 | None:intergenic |
| TAGGCATTGAAGATCGGCTT+TGG | 0.464541 | 4.4:+38336134 | None:intergenic |
| ATTAACCAACATCATGGTAT+TGG | 0.470830 | 4.4:+38336272 | None:intergenic |
| TAACAGGAAGTTCCATGGCT+TGG | 0.486774 | 4.4:+38336061 | None:intergenic |
| TATGATTGTGGCGATGTTGT+TGG | 0.507427 | 4.4:-38336301 | MS.gene45710:CDS |
| TATGTCGTTATAAGGATTCA+GGG | 0.530226 | 4.4:-38336113 | MS.gene45710:CDS |
| CTGAATCCTTATAACGACAT+AGG | 0.550946 | 4.4:+38336115 | None:intergenic |
| AGAGATGGAGGCATACAAGC+AGG | 0.551353 | 4.4:-38336325 | None:intergenic |
| AACAAGGACTGAAGGCATGC+GGG | 0.556452 | 4.4:-38336224 | MS.gene45710:CDS |
| GCCGTCTGATGCTTGCTGCT+CGG | 0.561297 | 4.4:-38336163 | MS.gene45710:CDS |
| ACAGTTAACAGGAAGTTCCA+TGG | 0.568806 | 4.4:+38336056 | None:intergenic |
| CTGACCATTAACCAACATCA+TGG | 0.570185 | 4.4:+38336266 | None:intergenic |
| GCCGAGCAGCAAGCATCAGA+CGG | 0.582886 | 4.4:+38336162 | None:intergenic |
| ACGACATAGGCATTGAAGAT+CGG | 0.583458 | 4.4:+38336128 | None:intergenic |
| GAATTGTCACCACTCACATA+CGG | 0.587779 | 4.4:+38336201 | None:intergenic |
| CATGACAAAACAAGGACTGA+AGG | 0.588836 | 4.4:-38336232 | MS.gene45710:CDS |
| ATACAAGCAGGTTATGATTG+TGG | 0.602790 | 4.4:-38336313 | MS.gene45710:CDS |
| CAACATCATGGTATTGGCAA+TGG | 0.605291 | 4.4:+38336278 | None:intergenic |
| CATTGCCAATACCATGATGT+TGG | 0.609484 | 4.4:-38336277 | MS.gene45710:CDS |
| TTATGTCACATGACAAAACA+AGG | 0.627195 | 4.4:-38336240 | MS.gene45710:CDS |
| AAACAAGGACTGAAGGCATG+CGG | 0.631589 | 4.4:-38336225 | MS.gene45710:CDS |
| TATTGATCCTAACCAAGCCA+TGG | 0.632800 | 4.4:-38336073 | MS.gene45710:CDS |
| AACCACATGACTAGTAGCAG+TGG | 0.639755 | 4.4:+38336016 | None:intergenic |
| GCATGCGGGCCGTATGTGAG+TGG | 0.668512 | 4.4:-38336210 | MS.gene45710:CDS |
| ACATGACTAGTAGCAGTGGA+AGG | 0.673463 | 4.4:+38336020 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTAACCAACATCATGGTAT+TGG | + | chr4.4:38336078-38336097 | None:intergenic | 30.0% | |
| TATGTCGTTATAAGGATTCA+GGG | - | chr4.4:38336234-38336253 | MS.gene45710:CDS | 30.0% | |
| TTATGTCACATGACAAAACA+AGG | - | chr4.4:38336107-38336126 | MS.gene45710:CDS | 30.0% | |
| ! | AATACCATGATGTTGGTTAA+TGG | - | chr4.4:38336077-38336096 | MS.gene45710:CDS | 30.0% |
| AGTGGTGACAATTCTGTTAA+TGG | - | chr4.4:38336155-38336174 | MS.gene45710:CDS | 35.0% | |
| ATACAAGCAGGTTATGATTG+TGG | - | chr4.4:38336034-38336053 | MS.gene45710:CDS | 35.0% | |
| CTATGTCGTTATAAGGATTC+AGG | - | chr4.4:38336233-38336252 | MS.gene45710:CDS | 35.0% | |
| CTGAATCCTTATAACGACAT+AGG | + | chr4.4:38336235-38336254 | None:intergenic | 35.0% | |
| TCAATGCCTATGTCGTTATA+AGG | - | chr4.4:38336226-38336245 | MS.gene45710:CDS | 35.0% | |
| TCCATAAGCTTACAGTTAAC+AGG | + | chr4.4:38336305-38336324 | None:intergenic | 35.0% | |
| TCCTGTTAACTGTAAGCTTA+TGG | - | chr4.4:38336301-38336320 | MS.gene45710:CDS | 35.0% | |
| ACAGTTAACAGGAAGTTCCA+TGG | + | chr4.4:38336294-38336313 | None:intergenic | 40.0% | |
| CATGACAAAACAAGGACTGA+AGG | - | chr4.4:38336115-38336134 | MS.gene45710:CDS | 40.0% | |
| CATTGCCAATACCATGATGT+TGG | - | chr4.4:38336070-38336089 | MS.gene45710:CDS | 40.0% | |
| CTGACCATTAACCAACATCA+TGG | + | chr4.4:38336084-38336103 | None:intergenic | 40.0% | |
| GAATTGTCACCACTCACATA+CGG | + | chr4.4:38336149-38336168 | None:intergenic | 40.0% | |
| TATGATTGTGGCGATGTTGT+TGG | - | chr4.4:38336046-38336065 | MS.gene45710:CDS | 40.0% | |
| TATTGATCCTAACCAAGCCA+TGG | - | chr4.4:38336274-38336293 | MS.gene45710:CDS | 40.0% | |
| ! | TCAGGGTTGCTTTCTTTCTA+TGG | - | chr4.4:38336251-38336270 | MS.gene45710:CDS | 40.0% |
| !! | ACGACATAGGCATTGAAGAT+CGG | + | chr4.4:38336222-38336241 | None:intergenic | 40.0% |
| !! | CAACATCATGGTATTGGCAA+TGG | + | chr4.4:38336072-38336091 | None:intergenic | 40.0% |
| TAACAGGAAGTTCCATGGCT+TGG | + | chr4.4:38336289-38336308 | None:intergenic | 45.0% | |
| ! | AAACAAGGACTGAAGGCATG+CGG | - | chr4.4:38336122-38336141 | MS.gene45710:CDS | 45.0% |
| !! | TAGGCATTGAAGATCGGCTT+TGG | + | chr4.4:38336216-38336235 | None:intergenic | 45.0% |
| ! | AACAAGGACTGAAGGCATGC+GGG | - | chr4.4:38336123-38336142 | MS.gene45710:CDS | 50.0% |
| !! | GGAAGTTCCATGGCTTGGTT+AGG | + | chr4.4:38336284-38336303 | None:intergenic | 50.0% |
| GCCGAGCAGCAAGCATCAGA+CGG | + | chr4.4:38336188-38336207 | None:intergenic | 60.0% | |
| ! | GCCGTCTGATGCTTGCTGCT+CGG | - | chr4.4:38336184-38336203 | MS.gene45710:CDS | 60.0% |
| GCATGCGGGCCGTATGTGAG+TGG | - | chr4.4:38336137-38336156 | MS.gene45710:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 38336026 | 38336343 | 38336026 | ID=MS.gene45710 |
| chr4.4 | mRNA | 38336026 | 38336343 | 38336026 | ID=MS.gene45710.t1;Parent=MS.gene45710 |
| chr4.4 | exon | 38336026 | 38336343 | 38336026 | ID=MS.gene45710.t1.exon1;Parent=MS.gene45710.t1 |
| chr4.4 | CDS | 38336026 | 38336343 | 38336026 | ID=cds.MS.gene45710.t1;Parent=MS.gene45710.t1 |
| Gene Sequence |
| Protein sequence |
