AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45780.t1 MTR_8g038250 88.155 439 28 2 1 438 1 416 0.0 768
MS.gene45780.t1 MTR_8g096990 78.082 438 90 2 1 438 1 432 0.0 702
MS.gene45780.t1 MTR_5g080890 73.923 441 105 6 1 438 1 434 0.0 648
MS.gene45780.t1 MTR_5g080960 73.696 441 106 6 1 438 1 434 0.0 647
MS.gene45780.t1 MTR_8g038280 71.719 442 115 3 1 438 1 436 0.0 645
MS.gene45780.t1 MTR_5g044770 70.548 438 122 3 1 438 1 431 0.0 619
MS.gene45780.t1 MTR_6g090290 64.480 442 146 4 1 437 1 436 0.0 553
MS.gene45780.t1 MTR_8g038310 60.502 438 147 5 1 437 1 413 0.0 525
MS.gene45780.t1 MTR_4g109760 65.148 439 112 7 1 438 1 399 0.0 513
MS.gene45780.t1 MTR_3g028120 53.095 420 187 6 25 438 24 439 9.67e-149 430
MS.gene45780.t1 MTR_5g038360 50.569 439 203 7 5 437 6 436 2.61e-142 414
MS.gene45780.t1 MTR_2g067830 52.670 412 183 7 32 437 1 406 6.14e-138 401
MS.gene45780.t1 MTR_6g088760 52.463 406 177 8 1 395 1 401 1.27e-135 399
MS.gene45780.t1 MTR_5g044570 47.865 445 190 10 1 437 1 411 4.18e-129 379
MS.gene45780.t1 MTR_0024s0040 49.640 417 194 8 30 437 1 410 4.16e-125 369
MS.gene45780.t1 MTR_2g105300 49.389 409 173 8 32 434 1 381 4.65e-123 363
MS.gene45780.t1 MTR_0036s0050 45.283 424 205 11 26 437 27 435 6.47e-113 338
MS.gene45780.t1 MTR_0036s0070 42.823 418 215 10 26 437 27 426 1.12e-108 327
MS.gene45780.t1 MTR_0036s0110 40.492 447 239 11 6 437 8 442 1.70e-101 310
MS.gene45780.t1 MTR_5g008070 42.180 422 219 10 21 436 23 425 4.49e-100 305
MS.gene45780.t1 MTR_2g059490 40.465 430 218 9 14 438 15 411 8.08e-94 290
MS.gene45780.t1 MTR_2g059460 40.047 422 206 10 23 437 25 406 3.39e-92 285
MS.gene45780.t1 MTR_5g008150 39.573 422 213 11 22 437 27 412 4.04e-90 280
MS.gene45780.t1 MTR_5g008110 38.568 433 219 12 12 437 23 415 1.87e-89 278
MS.gene45780.t1 MTR_3g097150 35.590 458 255 17 4 438 9 449 7.82e-75 241
MS.gene45780.t1 MTR_1g060750 37.284 405 217 12 40 437 2 376 2.12e-74 238
MS.gene45780.t1 MTR_4g045857 42.188 256 141 4 61 312 36 288 1.19e-60 199
MS.gene45780.t1 MTR_5g008090 37.086 302 176 8 142 437 21 314 4.03e-57 191
MS.gene45780.t1 MTR_7g117430 33.879 428 243 15 28 435 77 484 1.36e-55 191
MS.gene45780.t1 MTR_1g115210 35.556 360 205 13 85 435 145 486 7.05e-54 187
MS.gene45780.t1 MTR_1g039270 34.160 363 207 16 85 435 127 469 1.45e-49 175
MS.gene45780.t1 MTR_3g046300 31.783 387 244 9 68 438 79 461 8.84e-48 170
MS.gene45780.t1 MTR_1g111350 34.540 359 206 13 85 435 130 467 1.94e-47 169
MS.gene45780.t1 MTR_6g085010 34.366 355 209 13 86 435 156 491 7.77e-47 168
MS.gene45780.t1 MTR_6g088765 40.756 238 107 5 163 399 5 209 1.00e-44 155
MS.gene45780.t1 MTR_5g091130 31.538 390 242 11 68 438 173 556 6.89e-43 159
MS.gene45780.t1 MTR_2g028190 33.973 365 213 13 85 435 101 451 5.96e-42 154
MS.gene45780.t1 MTR_3g053710 32.231 363 222 11 85 435 116 466 1.45e-39 148
MS.gene45780.t1 MTR_4g007740 29.877 405 247 14 49 436 94 478 3.96e-39 147
MS.gene45780.t1 MTR_8g103880 29.609 358 226 13 87 436 117 456 1.45e-36 139
MS.gene45780.t1 MTR_2g070430 27.966 472 281 19 5 437 6 457 3.75e-35 135
MS.gene45780.t1 MTR_7g080230 27.586 435 273 16 30 436 11 431 1.08e-34 135
MS.gene45780.t1 MTR_2g055840 29.319 382 233 12 78 435 5 373 1.59e-34 132
MS.gene45780.t1 MTR_1g078240 27.861 402 255 14 58 436 41 430 1.22e-33 132
MS.gene45780.t1 MTR_1g078240 28.288 403 253 15 58 436 41 431 4.29e-33 130
MS.gene45780.t1 MTR_7g105850 29.193 483 266 22 9 437 7 467 9.88e-33 129
MS.gene45780.t1 MTR_3g068000 28.982 383 219 15 89 435 76 441 2.17e-31 125
MS.gene45780.t1 MTR_7g021510 29.381 388 221 19 82 436 67 434 7.10e-31 124
MS.gene45780.t1 MTR_3g065160 26.344 372 235 12 88 438 76 429 9.78e-30 120
MS.gene45780.t1 MTR_4g008110 55.752 113 27 1 162 274 48 137 1.41e-29 112
MS.gene45780.t1 MTR_3g053980 32.231 363 216 13 85 435 141 485 7.90e-29 118
MS.gene45780.t1 MTR_4g095270 25.598 418 263 17 41 438 37 426 4.78e-28 117
MS.gene45780.t1 MTR_4g095270 25.598 418 263 17 41 438 37 426 5.45e-28 117
MS.gene45780.t1 MTR_5g016260 25.558 403 242 16 57 438 69 434 1.28e-27 115
MS.gene45780.t1 MTR_5g016260 25.558 403 242 16 57 438 69 434 1.32e-27 115
MS.gene45780.t1 MTR_8g094590 26.952 397 245 14 65 438 53 427 1.90e-27 114
MS.gene45780.t1 MTR_4g056420 29.894 378 223 16 85 436 76 437 5.23e-27 113
MS.gene45780.t1 MTR_7g062360 27.865 384 226 16 89 438 62 428 4.30e-26 110
MS.gene45780.t1 MTR_3g096930 26.154 390 237 15 78 438 70 437 5.22e-26 110
MS.gene45780.t1 MTR_1g078240 27.976 336 210 12 58 373 41 364 1.26e-25 107
MS.gene45780.t1 MTR_5g077850 26.247 381 228 15 88 438 83 440 1.54e-25 108
MS.gene45780.t1 MTR_2g063110 26.055 403 235 16 85 435 117 508 1.95e-25 108
MS.gene45780.t1 MTR_7g080230 28.161 348 215 14 110 436 28 361 2.63e-25 107
MS.gene45780.t1 MTR_1g029810 25.798 376 238 14 85 438 56 412 5.81e-25 106
MS.gene45780.t1 MTR_1g029810 25.798 376 238 14 85 438 56 412 6.04e-25 106
MS.gene45780.t1 MTR_1g037630 28.537 410 263 16 25 423 26 416 9.56e-25 106
MS.gene45780.t1 MTR_4g023650 26.179 424 254 18 50 438 23 422 1.24e-24 105
MS.gene45780.t1 MTR_1g061940 26.797 459 286 20 8 436 7 445 1.49e-24 106
MS.gene45780.t1 MTR_6g084710 27.083 384 233 16 80 435 186 550 1.97e-24 106
MS.gene45780.t1 MTR_2g084550 30.263 380 220 17 85 436 76 438 2.45e-24 105
MS.gene45780.t1 MTR_4g023630 25.952 420 260 16 50 438 23 422 2.97e-24 104
MS.gene45780.t1 MTR_8g039540 28.605 430 271 17 12 423 10 421 6.79e-24 103
MS.gene45780.t1 MTR_7g102750 28.497 386 218 19 85 436 80 441 1.75e-23 103
MS.gene45780.t1 MTR_8g094590 30.686 277 165 10 65 334 53 309 2.45e-23 100
MS.gene45780.t1 MTR_3g014290 30.994 342 213 13 87 423 81 404 2.84e-23 101
MS.gene45780.t1 MTR_8g094590 29.720 286 174 10 65 343 53 318 1.46e-22 99.4
MS.gene45780.t1 MTR_0945s0010 38.760 129 74 4 311 438 1 125 3.83e-22 92.8
MS.gene45780.t1 MTR_6g084710 29.392 296 180 14 80 365 186 462 8.67e-22 98.2
MS.gene45780.t1 MTR_2g103340 38.686 137 40 1 20 156 33 125 2.60e-21 91.7
MS.gene45780.t1 MTR_2g103340 46.341 82 34 3 351 432 88 159 1.34e-11 63.9
MS.gene45780.t1 MTR_4g100990 26.923 416 225 19 86 438 74 473 1.31e-20 94.4
MS.gene45780.t1 MTR_7g102750 29.321 324 181 15 85 379 80 384 5.07e-20 92.4
MS.gene45780.t1 MTR_5g061690 29.296 355 198 20 93 423 108 433 6.22e-20 92.4
MS.gene45780.t1 MTR_8g075090 27.895 380 214 20 87 437 113 461 2.18e-18 87.8
MS.gene45780.t1 MTR_8g075090 27.822 381 215 20 87 438 113 462 2.83e-18 87.0
MS.gene45780.t1 MTR_5g077920 26.738 374 235 13 88 437 70 428 2.62e-17 84.0
MS.gene45780.t1 MTR_8g075090 30.164 305 169 15 92 374 118 400 2.98e-17 84.0
MS.gene45780.t1 MTR_3g006820 23.750 400 264 15 64 438 32 415 3.61e-16 80.5
MS.gene45780.t1 MTR_8g075010 26.042 384 220 19 87 438 101 452 1.57e-14 75.9
MS.gene45780.t1 MTR_2g037710 27.650 217 124 7 228 436 15 206 3.81e-14 71.6
MS.gene45780.t1 MTR_5g080330 24.793 363 201 17 103 435 2 322 5.82e-14 73.9
MS.gene45780.t1 MTR_3g100500 27.913 412 253 20 30 422 38 424 6.15e-12 67.4
MS.gene45780.t1 MTR_3g087780 26.488 336 190 19 128 438 8 311 3.44e-11 65.1
MS.gene45780.t1 MTR_3g087780 26.048 334 194 17 128 438 44 347 4.11e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45780.t1 AT1G64830 51.208 414 195 3 26 438 24 431 1.59e-140 409
MS.gene45780.t1 AT5G33340 52.415 414 189 4 27 438 30 437 4.61e-137 400
MS.gene45780.t1 AT2G35615 45.475 453 221 9 1 436 1 444 1.00e-117 351
MS.gene45780.t1 AT1G31450 43.400 447 226 8 4 436 9 442 4.66e-108 327
MS.gene45780.t1 AT2G28010 40.868 438 206 12 5 438 6 394 3.61e-93 287
MS.gene45780.t1 AT2G28040 41.412 425 198 12 16 438 18 393 5.34e-91 281
MS.gene45780.t1 AT2G28040 41.827 416 189 12 26 438 19 384 1.28e-89 277
MS.gene45780.t1 AT2G28030 43.478 368 178 10 75 438 49 390 1.15e-85 267
MS.gene45780.t1 AT2G28220 38.837 430 211 11 15 438 17 400 1.50e-83 262
MS.gene45780.t1 AT2G28225 40.701 371 188 8 75 438 55 400 2.55e-79 251
MS.gene45780.t1 AT2G03200 36.364 440 234 15 25 438 42 461 6.50e-71 231
MS.gene45780.t1 AT1G01300 37.673 361 201 13 85 436 140 485 2.81e-55 191
MS.gene45780.t1 AT3G61820 36.294 394 220 14 55 435 108 483 7.71e-54 187
MS.gene45780.t1 AT4G30030 36.508 378 211 15 72 438 63 422 8.43e-54 185
MS.gene45780.t1 AT3G18490 35.013 377 218 14 68 435 142 500 8.64e-53 184
MS.gene45780.t1 AT2G23945 27.669 459 286 14 9 438 10 451 1.43e-48 172
MS.gene45780.t1 AT5G10760 32.791 430 251 15 22 435 57 464 7.43e-48 171
MS.gene45780.t1 AT1G25510 33.973 365 200 15 85 435 146 483 8.74e-47 168
MS.gene45780.t1 AT3G20015 30.317 442 244 18 28 435 59 470 1.05e-45 165
MS.gene45780.t1 AT5G36260 32.275 378 211 15 85 436 76 434 1.15e-45 165
MS.gene45780.t1 AT3G25700 30.374 428 252 16 39 436 39 450 1.37e-45 164
MS.gene45780.t1 AT5G10770 32.880 441 250 17 22 436 54 474 1.07e-44 162
MS.gene45780.t1 AT3G02740 31.200 375 218 14 85 436 83 440 1.03e-41 154
MS.gene45780.t1 AT3G02740 30.563 373 219 13 87 436 10 365 1.64e-41 152
MS.gene45780.t1 AT2G42980 32.134 389 236 13 72 438 145 527 1.26e-39 149
MS.gene45780.t1 AT3G59080 31.443 388 238 14 72 438 155 535 3.47e-39 148
MS.gene45780.t1 AT4G30040 30.310 452 251 22 9 430 10 427 5.89e-39 145
MS.gene45780.t1 AT1G65240 29.777 403 239 14 59 436 44 427 4.95e-38 144
MS.gene45780.t1 AT3G12700 27.790 457 284 18 5 436 26 461 1.44e-37 142
MS.gene45780.t1 AT1G79720 32.877 365 212 15 87 435 135 482 1.70e-35 137
MS.gene45780.t1 AT2G39710 29.787 376 213 15 89 430 67 425 8.58e-34 132
MS.gene45780.t1 AT5G37540 28.000 375 226 14 88 435 81 438 8.98e-32 126
MS.gene45780.t1 AT3G59080 29.688 384 214 13 72 438 155 499 5.55e-31 124
MS.gene45780.t1 AT1G66180 26.260 377 232 14 88 438 73 429 2.71e-30 122
MS.gene45780.t1 AT5G43100 28.307 378 218 19 84 438 73 420 1.97e-28 117
MS.gene45780.t1 AT3G54400 28.093 388 250 13 61 436 55 425 2.17e-28 116
MS.gene45780.t1 AT5G43100 28.307 378 218 19 84 438 73 420 2.78e-28 118
MS.gene45780.t1 AT4G33490 24.515 412 252 13 61 438 28 414 2.94e-28 116
MS.gene45780.t1 AT5G02190 26.862 376 224 15 96 435 82 442 6.07e-28 115
MS.gene45780.t1 AT1G05840 28.000 375 234 15 85 436 78 439 4.52e-27 113
MS.gene45780.t1 AT3G50050 26.226 469 262 22 12 438 13 439 2.16e-26 112
MS.gene45780.t1 AT4G12920 27.013 385 242 13 61 435 32 387 1.07e-25 108
MS.gene45780.t1 AT1G09750 28.997 369 222 16 85 435 102 448 1.60e-25 108
MS.gene45780.t1 AT1G44130 25.815 399 248 14 68 438 24 402 1.68e-25 107
MS.gene45780.t1 AT5G22850 29.288 379 225 15 85 436 79 441 4.65e-25 107
MS.gene45780.t1 AT5G45120 28.571 406 223 18 87 435 83 478 1.37e-24 106
MS.gene45780.t1 AT2G36670 27.512 418 244 19 59 436 60 458 6.99e-24 104
MS.gene45780.t1 AT2G36670 27.187 423 244 19 59 436 60 463 3.98e-23 102
MS.gene45780.t1 AT2G36670 27.733 375 230 15 87 436 34 392 4.73e-23 101
MS.gene45780.t1 AT2G36670 27.733 375 230 15 87 436 34 392 4.73e-23 101
MS.gene45780.t1 AT4G33490 27.869 305 179 11 61 349 25 304 6.89e-23 100
MS.gene45780.t1 AT1G77480 26.371 383 228 17 85 438 65 422 1.46e-22 99.8
MS.gene45780.t1 AT1G77480 26.371 383 228 17 85 438 65 422 1.98e-22 99.8
MS.gene45780.t1 AT2G17760 27.483 433 248 21 32 435 54 449 4.69e-22 99.0
MS.gene45780.t1 AT3G52500 28.894 398 222 21 84 436 87 468 6.70e-22 98.2
MS.gene45780.t1 AT5G07030 27.586 464 284 18 2 436 15 455 9.16e-22 97.8
MS.gene45780.t1 AT1G08210 27.586 377 231 15 85 436 130 489 6.18e-21 95.9
MS.gene45780.t1 AT1G08210 27.586 377 231 15 85 436 116 475 6.59e-21 95.5
MS.gene45780.t1 AT1G08210 27.586 377 231 15 85 436 82 441 6.84e-21 95.5
MS.gene45780.t1 AT1G49050 22.802 364 234 12 105 437 50 397 2.52e-20 92.8
MS.gene45780.t1 AT1G49050 25.385 390 243 15 80 437 197 570 2.61e-20 94.0
MS.gene45780.t1 AT3G51350 24.888 446 275 18 27 435 30 452 7.16e-19 89.4
MS.gene45780.t1 AT3G42550 23.678 397 236 12 55 436 46 390 8.91e-18 85.5
MS.gene45780.t1 AT1G49050 27.599 279 169 12 80 341 197 459 1.55e-17 85.1
MS.gene45780.t1 AT1G08210 26.303 403 230 16 85 436 82 468 8.62e-17 82.8
MS.gene45780.t1 AT3G25700 27.106 273 156 10 39 293 39 286 3.11e-16 80.1
MS.gene45780.t1 AT5G10080 25.156 481 279 27 8 437 5 455 4.51e-15 77.8
MS.gene45780.t1 AT5G24820 23.650 389 248 12 74 435 33 399 2.47e-14 74.7
MS.gene45780.t1 AT3G51340 23.691 363 235 13 92 435 108 447 3.57e-14 74.7
MS.gene45780.t1 AT3G51360 26.146 371 218 18 92 435 10 351 4.69e-13 70.9

Find 90 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGGTGTTTATAATAATT+TGG 0.122254 8.3:-58596050 MS.gene45780:CDS
GGAGATGCCGCTGTGGTTTC+CGG 0.148862 8.3:-58596244 MS.gene45780:CDS
TTAAAATGTGCCGTTATTAT+AGG 0.181038 8.3:+58595929 None:intergenic
GGTTCTGACCTTTCCATCTT+TGG 0.183882 8.3:-58595812 MS.gene45780:CDS
ACGACAAGCAAACTCAATTT+TGG 0.203851 8.3:-58596265 MS.gene45780:CDS
GGTTGGTATAGTGGTGATTT+TGG 0.223826 8.3:+58596907 None:intergenic
ACCCCACCATTTAAGTTATA+TGG 0.242744 8.3:-58596736 MS.gene45780:CDS
GTCAACCCCTATAGTGAAAA+AGG 0.271435 8.3:-58596209 MS.gene45780:CDS
GCAATGCCATATAACTTAAA+TGG 0.273035 8.3:+58596730 None:intergenic
AACAAAAGAATAGAATTTAA+AGG 0.289730 8.3:-58596139 MS.gene45780:CDS
TCATTTCCTAAAACTGTGAT+AGG 0.290097 8.3:-58596439 MS.gene45780:CDS
GAGATGCCGCTGTGGTTTCC+GGG 0.323952 8.3:-58596243 MS.gene45780:CDS
CTTGTCGTGTTTGACTCAAC+CGG 0.337489 8.3:+58596280 None:intergenic
ACAGCAACAAATAAACTAAT+AGG 0.342697 8.3:+58595884 None:intergenic
AGCGTCTCTTATTACACAAT+TGG 0.348748 8.3:-58596347 MS.gene45780:CDS
GGTAACATCATAATTGATTC+TGG 0.364728 8.3:-58596094 MS.gene45780:CDS
TATAGCAAAGGTTAAATTCT+CGG 0.388913 8.3:+58595978 None:intergenic
CTTAGCTCAGCAGAATTTGT+TGG 0.389893 8.3:-58595786 MS.gene45780:CDS
GCAGTTACTTCTGGGTTCCC+TGG 0.393771 8.3:-58595833 MS.gene45780:CDS
AGCTCCGGTGTAGTTGGCCT+TGG 0.399332 8.3:-58596379 MS.gene45780:CDS
GCCGCTGTGGTTTCCGGGGA+TGG 0.421556 8.3:-58596238 MS.gene45780:CDS
GTCAAATAGTAGAAAACTAT+AGG 0.426026 8.3:+58596184 None:intergenic
GTGTTTGACTCAACCGGGTT+TGG 0.428845 8.3:+58596286 None:intergenic
TTTAAAGGGTCTTCAAATAG+TGG 0.429034 8.3:-58596124 MS.gene45780:CDS
TTGATTGAGGTGTGTTTATG+AGG 0.430036 8.3:+58596806 None:intergenic
GGAATCAGCTGTGTTGGAAT+TGG 0.432066 8.3:-58596029 MS.gene45780:CDS
ATAGCAAAGGTTAAATTCTC+GGG 0.433218 8.3:+58595979 None:intergenic
TCCGGTGTAGTTGGCCTTGG+AGG 0.448012 8.3:-58596376 MS.gene45780:CDS
ACAAAAGAATAGAATTTAAA+GGG 0.448273 8.3:-58596138 MS.gene45780:CDS
GTTCAAACTTAGGAGTGGTT+TGG 0.448701 8.3:+58596650 None:intergenic
CTGAGCTAAGTTTCCAAAGA+TGG 0.451967 8.3:+58595799 None:intergenic
ATGAGATACTCACCATGATC+GGG 0.452999 8.3:+58596775 None:intergenic
TGGTGGGGTACCAACTGAAT+AGG 0.458426 8.3:+58596750 None:intergenic
GACAATACAATGTCATTTGA+AGG 0.463678 8.3:-58596406 MS.gene45780:CDS
ATACAGGTAGTGACATCGTT+TGG 0.467751 8.3:-58596705 MS.gene45780:CDS
ACGTTGACAGTTATTCCAAC+TGG 0.472429 8.3:-58596067 MS.gene45780:CDS
CCTAAAACTGTGATAGGATG+TGG 0.472735 8.3:-58596433 MS.gene45780:CDS
AAGAGAAATAATGAAACAAA+GGG 0.474915 8.3:+58596981 None:intergenic
TAATTTGGAATCAGCTGTGT+TGG 0.477151 8.3:-58596035 MS.gene45780:CDS
AAATTATTATAAACACCAGT+TGG 0.480158 8.3:+58596052 None:intergenic
GGTGTAGTTGGCCTTGGAGG+TGG 0.481284 8.3:-58596373 MS.gene45780:CDS
GCGTCTCTTATTACACAATT+GGG 0.481758 8.3:-58596346 MS.gene45780:CDS
TTATATGGCATTGCTGATAC+AGG 0.486441 8.3:-58596721 MS.gene45780:CDS
GATTGGCACGGTTTCTAGAA+CGG 0.494681 8.3:+58596845 None:intergenic
CTTGTTGCACAGATTTACAT+AGG 0.501571 8.3:+58596584 None:intergenic
TGGTTGTAACATTCTTTGCA+AGG 0.508371 8.3:+58596670 None:intergenic
GGTGCAAGCTCCGGTGTAGT+TGG 0.509113 8.3:-58596385 MS.gene45780:CDS
TGCCATATAACTTAAATGGT+GGG 0.513388 8.3:+58596734 None:intergenic
TGCTATAGTGTCACATCAGA+TGG 0.514224 8.3:-58595962 MS.gene45780:CDS
CAAAGGTTAAATTCTCGGGT+TGG 0.519243 8.3:+58595983 None:intergenic
TTATTGTTTGTTGCCAAACC+CGG 0.519426 8.3:-58596299 MS.gene45780:CDS
ACCTCCAAGGCCAACTACAC+CGG 0.524986 8.3:+58596375 None:intergenic
TCATTTGAAGGTGCAAGCTC+CGG 0.527243 8.3:-58596394 MS.gene45780:CDS
TTGTCGTGTTTGACTCAACC+GGG 0.535482 8.3:+58596281 None:intergenic
CATGAGATACTCACCATGAT+CGG 0.536629 8.3:+58596774 None:intergenic
AAAGAGAAATAATGAAACAA+AGG 0.537812 8.3:+58596980 None:intergenic
TTATTTGTTGCTGTTGCAGA+TGG 0.545033 8.3:-58595875 MS.gene45780:CDS
CTTTCTCGTGCTTTAAACAA+CGG 0.547127 8.3:-58596961 MS.gene45780:CDS
TCAACTGTAATCCCCGATCA+TGG 0.547516 8.3:-58596787 MS.gene45780:CDS
ACATTGGAAGCATTTAGTGT+TGG 0.556371 8.3:-58596163 MS.gene45780:CDS
AAGATGGAAAGGTCAGAACC+AGG 0.560391 8.3:+58595815 None:intergenic
GCTCAGCAGAATTTGTTGGT+TGG 0.563160 8.3:-58595782 MS.gene45780:CDS
GTCTTCAAATAGTGGTGATG+AGG 0.563185 8.3:-58596116 MS.gene45780:CDS
ATGCCATATAACTTAAATGG+TGG 0.567034 8.3:+58596733 None:intergenic
TCGGGGATTACAGTTGATTG+AGG 0.568413 8.3:+58596793 None:intergenic
CTAAGTTTCCAAAGATGGAA+AGG 0.568814 8.3:+58595804 None:intergenic
TACACTTTGCTACAATCAGT+AGG 0.577745 8.3:+58595725 None:intergenic
GCCATATAACTTAAATGGTG+GGG 0.578953 8.3:+58596735 None:intergenic
GTGCGGCATTGACAATATGT+TGG 0.580612 8.3:+58596869 None:intergenic
GGTTGACACTACACCATCCC+CGG 0.582467 8.3:+58596225 None:intergenic
AACAAAGGGAGAAGTAAAAG+AGG 0.583827 8.3:+58596995 None:intergenic
GAGACGCTGGTCCACCTCCA+AGG 0.588914 8.3:+58596362 None:intergenic
TGATGGTTCAAACTTAGGAG+TGG 0.603375 8.3:+58596645 None:intergenic
TCTTCAAATAGTGGTGATGA+GGG 0.615556 8.3:-58596115 MS.gene45780:CDS
GGTGATACGTCACAATCGAA+AGG 0.619221 8.3:-58596514 MS.gene45780:CDS
CTTACATTAGAGTCCTCCAC+TGG 0.621526 8.3:-58596472 MS.gene45780:CDS
AAATGAAATAGGACGTCCAG+TGG 0.627128 8.3:+58596456 None:intergenic
CTGATGTGACACTATAGCAA+AGG 0.629182 8.3:+58595966 None:intergenic
AGATGCCGCTGTGGTTTCCG+GGG 0.633627 8.3:-58596242 MS.gene45780:CDS
TTACATAGGTCAGACGAACA+TGG 0.633649 8.3:+58596598 None:intergenic
TATCTCATGACCTATTCAGT+TGG 0.633970 8.3:-58596760 MS.gene45780:CDS
TGATTGAGGTGTGTTTATGA+GGG 0.635382 8.3:+58596807 None:intergenic
AGATGGAAAGGTCAGAACCA+GGG 0.650286 8.3:+58595816 None:intergenic
TGAAATAGGACGTCCAGTGG+AGG 0.654304 8.3:+58596459 None:intergenic
GAAGTAAAAGAGGCTCAGCA+AGG 0.655704 8.3:+58597005 None:intergenic
TGAGATACTCACCATGATCG+GGG 0.668047 8.3:+58596776 None:intergenic
ACGGTTTCTAGAACGGCGTG+CGG 0.669771 8.3:+58596852 None:intergenic
AATTGTGTAATAAGAGACGC+TGG 0.670336 8.3:+58596349 None:intergenic
CTATGTAAATCTGTGCAACA+AGG 0.670936 8.3:-58596583 MS.gene45780:CDS
ACCATCCCCGGAAACCACAG+CGG 0.698241 8.3:+58596237 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAGAATAGAATTTAA+AGG - chr8.3:58596603-58596622 MS.gene45780:CDS 15.0%
!! ACAAAAGAATAGAATTTAAA+GGG - chr8.3:58596604-58596623 MS.gene45780:CDS 15.0%
!!! AAAATTTTGATCATTACAAT+CGG + chr8.3:58596187-58596206 None:intergenic 15.0%
!!! ATATGATTTTCCTATAATAA+CGG - chr8.3:58596803-58596822 MS.gene45780:CDS 15.0%
!!! TGTGAATATAGTATTTTTTA+TGG - chr8.3:58596207-58596226 MS.gene45780:CDS 15.0%
!! AAAGAGAAATAATGAAACAA+AGG + chr8.3:58595765-58595784 None:intergenic 20.0%
!! AAATTATTATAAACACCAGT+TGG + chr8.3:58596693-58596712 None:intergenic 20.0%
!! AACTGGTGTTTATAATAATT+TGG - chr8.3:58596692-58596711 MS.gene45780:CDS 20.0%
!! AAGAGAAATAATGAAACAAA+GGG + chr8.3:58595764-58595783 None:intergenic 20.0%
! ACAGCAACAAATAAACTAAT+AGG + chr8.3:58596861-58596880 None:intergenic 25.0%
! GTCAAATAGTAGAAAACTAT+AGG + chr8.3:58596561-58596580 None:intergenic 25.0%
! TATAGCAAAGGTTAAATTCT+CGG + chr8.3:58596767-58596786 None:intergenic 25.0%
!! AGTTTTCTACTATTTGACAT+TGG - chr8.3:58596563-58596582 MS.gene45780:CDS 25.0%
!! ATAATAACGGCACATTTTAA+AGG - chr8.3:58596816-58596835 MS.gene45780:CDS 25.0%
!! TTAAAATGTGCCGTTATTAT+AGG + chr8.3:58596816-58596835 None:intergenic 25.0%
!!! ACAGTTTTAGGAAATGAAAT+AGG + chr8.3:58596300-58596319 None:intergenic 25.0%
ATAGCAAAGGTTAAATTCTC+GGG + chr8.3:58596766-58596785 None:intergenic 30.0%
ATGCCATATAACTTAAATGG+TGG + chr8.3:58596012-58596031 None:intergenic 30.0%
GCAATGCCATATAACTTAAA+TGG + chr8.3:58596015-58596034 None:intergenic 30.0%
TCATTTCCTAAAACTGTGAT+AGG - chr8.3:58596303-58596322 MS.gene45780:CDS 30.0%
TGCCATATAACTTAAATGGT+GGG + chr8.3:58596011-58596030 None:intergenic 30.0%
TTTAAAGGGTCTTCAAATAG+TGG - chr8.3:58596618-58596637 MS.gene45780:CDS 30.0%
! GACAATACAATGTCATTTGA+AGG - chr8.3:58596336-58596355 MS.gene45780:CDS 30.0%
! GGTAACATCATAATTGATTC+TGG - chr8.3:58596648-58596667 MS.gene45780:CDS 30.0%
! TTTTGTAGAAATGATTGGCA+CGG + chr8.3:58595912-58595931 None:intergenic 30.0%
!! ATAGGATCCTTTTTCACTAT+AGG + chr8.3:58596543-58596562 None:intergenic 30.0%
!! GATTTTGATGGTTCAAACTT+AGG + chr8.3:58596105-58596124 None:intergenic 30.0%
!! TAGGATCCTTTTTCACTATA+GGG + chr8.3:58596542-58596561 None:intergenic 30.0%
!! TTGTAAGTTGACGATTTTGA+TGG + chr8.3:58596117-58596136 None:intergenic 30.0%
!!! ACGACACAATTTTTTGTTGA+AGG + chr8.3:58596992-58597011 None:intergenic 30.0%
!!! TGTTGGTATTTGTTTTGTGT+AGG + chr8.3:58595859-58595878 None:intergenic 30.0%
AACAAAGGGAGAAGTAAAAG+AGG + chr8.3:58595750-58595769 None:intergenic 35.0%
AATTGTGTAATAAGAGACGC+TGG + chr8.3:58596396-58596415 None:intergenic 35.0%
AGCGTCTCTTATTACACAAT+TGG - chr8.3:58596395-58596414 MS.gene45780:CDS 35.0%
CTAAGTTTCCAAAGATGGAA+AGG + chr8.3:58596941-58596960 None:intergenic 35.0%
CTATGTAAATCTGTGCAACA+AGG - chr8.3:58596159-58596178 MS.gene45780:CDS 35.0%
CTTGTTGCACAGATTTACAT+AGG + chr8.3:58596161-58596180 None:intergenic 35.0%
GCCATATAACTTAAATGGTG+GGG + chr8.3:58596010-58596029 None:intergenic 35.0%
GCGTCTCTTATTACACAATT+GGG - chr8.3:58596396-58596415 MS.gene45780:CDS 35.0%
TACACTTTGCTACAATCAGT+AGG + chr8.3:58597020-58597039 None:intergenic 35.0%
TATCTCATGACCTATTCAGT+TGG - chr8.3:58595982-58596001 MS.gene45780:CDS 35.0%
TGATTGAGGTGTGTTTATGA+GGG + chr8.3:58595938-58595957 None:intergenic 35.0%
TGGTTGTAACATTCTTTGCA+AGG + chr8.3:58596075-58596094 None:intergenic 35.0%
TTATATGGCATTGCTGATAC+AGG - chr8.3:58596021-58596040 MS.gene45780:CDS 35.0%
TTGATTGAGGTGTGTTTATG+AGG + chr8.3:58595939-58595958 None:intergenic 35.0%
! ACCCCACCATTTAAGTTATA+TGG - chr8.3:58596006-58596025 MS.gene45780:CDS 35.0%
! ACGACAAGCAAACTCAATTT+TGG - chr8.3:58596477-58596496 MS.gene45780:CDS 35.0%
! CTTTCTCGTGCTTTAAACAA+CGG - chr8.3:58595781-58595800 MS.gene45780:CDS 35.0%
! GTTGGTATAGTGGTGATTTT+GGG + chr8.3:58595837-58595856 None:intergenic 35.0%
! TAATTTGGAATCAGCTGTGT+TGG - chr8.3:58596707-58596726 MS.gene45780:CDS 35.0%
! TCTTCAAATAGTGGTGATGA+GGG - chr8.3:58596627-58596646 MS.gene45780:CDS 35.0%
! TTATTGTTTGTTGCCAAACC+CGG - chr8.3:58596443-58596462 MS.gene45780:CDS 35.0%
! TTATTTGTTGCTGTTGCAGA+TGG - chr8.3:58596867-58596886 MS.gene45780:CDS 35.0%
! TTGGTATAGTGGTGATTTTG+GGG + chr8.3:58595836-58595855 None:intergenic 35.0%
!! ACATTGGAAGCATTTAGTGT+TGG - chr8.3:58596579-58596598 MS.gene45780:CDS 35.0%
!! AGGATCCTTTTTCACTATAG+GGG + chr8.3:58596541-58596560 None:intergenic 35.0%
!! GGCAGTTTTGTAGAAATGAT+TGG + chr8.3:58595917-58595936 None:intergenic 35.0%
!! TTGCTTTTGCAGTTACTTCT+GGG - chr8.3:58596901-58596920 MS.gene45780:CDS 35.0%
!! TTTGCTTTTGCAGTTACTTC+TGG - chr8.3:58596900-58596919 MS.gene45780:CDS 35.0%
!!! GGTATTTGTTTTGTGTAGGT+TGG + chr8.3:58595855-58595874 None:intergenic 35.0%
!!! TTTTGTGTAGGTTGGTATAG+TGG + chr8.3:58595847-58595866 None:intergenic 35.0%
AAATGAAATAGGACGTCCAG+TGG + chr8.3:58596289-58596308 None:intergenic 40.0%
ACGTTGACAGTTATTCCAAC+TGG - chr8.3:58596675-58596694 MS.gene45780:CDS 40.0%
ATACAGGTAGTGACATCGTT+TGG - chr8.3:58596037-58596056 MS.gene45780:CDS 40.0%
ATGAGATACTCACCATGATC+GGG + chr8.3:58595970-58595989 None:intergenic 40.0%
CAAAGGTTAAATTCTCGGGT+TGG + chr8.3:58596762-58596781 None:intergenic 40.0%
CATGAGATACTCACCATGAT+CGG + chr8.3:58595971-58595990 None:intergenic 40.0%
CCTAAAACTGTGATAGGATG+TGG - chr8.3:58596309-58596328 MS.gene45780:CDS 40.0%
CTGAGCTAAGTTTCCAAAGA+TGG + chr8.3:58596946-58596965 None:intergenic 40.0%
CTGATGTGACACTATAGCAA+AGG + chr8.3:58596779-58596798 None:intergenic 40.0%
CTTAGCTCAGCAGAATTTGT+TGG - chr8.3:58596956-58596975 MS.gene45780:CDS 40.0%
GTCAACCCCTATAGTGAAAA+AGG - chr8.3:58596533-58596552 MS.gene45780:CDS 40.0%
GTTCAAACTTAGGAGTGGTT+TGG + chr8.3:58596095-58596114 None:intergenic 40.0%
TGATGGTTCAAACTTAGGAG+TGG + chr8.3:58596100-58596119 None:intergenic 40.0%
TGCTATAGTGTCACATCAGA+TGG - chr8.3:58596780-58596799 MS.gene45780:CDS 40.0%
TTACATAGGTCAGACGAACA+TGG + chr8.3:58596147-58596166 None:intergenic 40.0%
! CCACATCCTATCACAGTTTT+AGG + chr8.3:58596312-58596331 None:intergenic 40.0%
! GTCTTCAAATAGTGGTGATG+AGG - chr8.3:58596626-58596645 MS.gene45780:CDS 40.0%
!! GGTTGGTATAGTGGTGATTT+TGG + chr8.3:58595838-58595857 None:intergenic 40.0%
AAGATGGAAAGGTCAGAACC+AGG + chr8.3:58596930-58596949 None:intergenic 45.0%
AGATGGAAAGGTCAGAACCA+GGG + chr8.3:58596929-58596948 None:intergenic 45.0%
CTTACATTAGAGTCCTCCAC+TGG - chr8.3:58596270-58596289 MS.gene45780:CDS 45.0%
CTTGTCGTGTTTGACTCAAC+CGG + chr8.3:58596465-58596484 None:intergenic 45.0%
GAAGTAAAAGAGGCTCAGCA+AGG + chr8.3:58595740-58595759 None:intergenic 45.0%
GGAATCAGCTGTGTTGGAAT+TGG - chr8.3:58596713-58596732 MS.gene45780:CDS 45.0%
GGTGATACGTCACAATCGAA+AGG - chr8.3:58596228-58596247 MS.gene45780:CDS 45.0%
TCAACTGTAATCCCCGATCA+TGG - chr8.3:58595955-58595974 MS.gene45780:CDS 45.0%
TCGGGGATTACAGTTGATTG+AGG + chr8.3:58595952-58595971 None:intergenic 45.0%
TGAGATACTCACCATGATCG+GGG + chr8.3:58595969-58595988 None:intergenic 45.0%
TTGTCGTGTTTGACTCAACC+GGG + chr8.3:58596464-58596483 None:intergenic 45.0%
! GATTGGCACGGTTTCTAGAA+CGG + chr8.3:58595900-58595919 None:intergenic 45.0%
! GGTTCTGACCTTTCCATCTT+TGG - chr8.3:58596930-58596949 MS.gene45780:CDS 45.0%
! TCATTTGAAGGTGCAAGCTC+CGG - chr8.3:58596348-58596367 MS.gene45780:CDS 45.0%
!! GCTCAGCAGAATTTGTTGGT+TGG - chr8.3:58596960-58596979 MS.gene45780:CDS 45.0%
!! GTGCGGCATTGACAATATGT+TGG + chr8.3:58595876-58595895 None:intergenic 45.0%
GTGTTTGACTCAACCGGGTT+TGG + chr8.3:58596459-58596478 None:intergenic 50.0%
TGAAATAGGACGTCCAGTGG+AGG + chr8.3:58596286-58596305 None:intergenic 50.0%
! TGGTGGGGTACCAACTGAAT+AGG + chr8.3:58595995-58596014 None:intergenic 50.0%
!! CAATTTTGGAGATGCCGCTG+TGG - chr8.3:58596491-58596510 MS.gene45780:CDS 50.0%
ACCTCCAAGGCCAACTACAC+CGG + chr8.3:58596370-58596389 None:intergenic 55.0%
GCAGTTACTTCTGGGTTCCC+TGG - chr8.3:58596909-58596928 MS.gene45780:CDS 55.0%
GGTTGACACTACACCATCCC+CGG + chr8.3:58596520-58596539 None:intergenic 55.0%
!! ACGGTTTCTAGAACGGCGTG+CGG + chr8.3:58595893-58595912 None:intergenic 55.0%
!! GTGGTGATTTTGGGGAGTCG+CGG + chr8.3:58595828-58595847 None:intergenic 55.0%
ACCATCCCCGGAAACCACAG+CGG + chr8.3:58596508-58596527 None:intergenic 60.0%
AGATGCCGCTGTGGTTTCCG+GGG - chr8.3:58596500-58596519 MS.gene45780:CDS 60.0%
GAGATGCCGCTGTGGTTTCC+GGG - chr8.3:58596499-58596518 MS.gene45780:CDS 60.0%
GGAGATGCCGCTGTGGTTTC+CGG - chr8.3:58596498-58596517 MS.gene45780:CDS 60.0%
GGTGCAAGCTCCGGTGTAGT+TGG - chr8.3:58596357-58596376 MS.gene45780:CDS 60.0%
! AGCTCCGGTGTAGTTGGCCT+TGG - chr8.3:58596363-58596382 MS.gene45780:CDS 60.0%
!! GGTGTAGTTGGCCTTGGAGG+TGG - chr8.3:58596369-58596388 MS.gene45780:CDS 60.0%
!! GTGATTTTGGGGAGTCGCGG+TGG + chr8.3:58595825-58595844 None:intergenic 60.0%
!! TCCGGTGTAGTTGGCCTTGG+AGG - chr8.3:58596366-58596385 MS.gene45780:CDS 60.0%
GAGACGCTGGTCCACCTCCA+AGG + chr8.3:58596383-58596402 None:intergenic 65.0%
GCCGCTGTGGTTTCCGGGGA+TGG - chr8.3:58596504-58596523 MS.gene45780:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 58595724 58597040 58595724 ID=MS.gene45780
chr8.3 mRNA 58595724 58597040 58595724 ID=MS.gene45780.t1;Parent=MS.gene45780
chr8.3 exon 58595724 58597040 58595724 ID=MS.gene45780.t1.exon1;Parent=MS.gene45780.t1
chr8.3 CDS 58595724 58597040 58595724 ID=cds.MS.gene45780.t1;Parent=MS.gene45780.t1
Gene Sequence

>MS.gene45780

ATGAATACATGTTCCTTGCTGAGCCTCTTTTACTTCTCCCTTTGTTTCATTATTTCTCTTTCTCGTGCTTTAAACAACGGTTTTAGTGTTGAACTCATCCACCGCGACTCCCCAAAATCACCACTATACCAACCTACACAAAACAAATACCAACATATTGTCAATGCCGCACGCCGTTCTAGAAACCGTGCCAATCATTTCTACAAAACTGCCCTCATAAACACACCTCAATCAACTGTAATCCCCGATCATGGTGAGTATCTCATGACCTATTCAGTTGGTACCCCACCATTTAAGTTATATGGCATTGCTGATACAGGTAGTGACATCGTTTGGCTTCAATGCGAGCCTTGCAAAGAATGTTACAACCAAACCACTCCTAAGTTTGAACCATCAAAATCGTCAACTTACAAAAACATTCCATGTTCGTCTGACCTATGTAAATCTGTGCAACAAGGTGCCGATTGTAATGATCAAAATTTTTGTGAATATAGTATTTTTTATGGTGATACGTCACAATCGAAAGGAAATCTTAGTGTCGATACTCTTACATTAGAGTCCTCCACTGGACGTCCTATTTCATTTCCTAAAACTGTGATAGGATGTGGAACTGACAATACAATGTCATTTGAAGGTGCAAGCTCCGGTGTAGTTGGCCTTGGAGGTGGACCAGCGTCTCTTATTACACAATTGGGATCATCAATTGATTCAAAATTCTCTTATTGTTTGTTGCCAAACCCGGTTGAGTCAAACACGACAAGCAAACTCAATTTTGGAGATGCCGCTGTGGTTTCCGGGGATGGTGTAGTGTCAACCCCTATAGTGAAAAAGGATCCTATAGTTTTCTACTATTTGACATTGGAAGCATTTAGTGTTGGTAACAAAAGAATAGAATTTAAAGGGTCTTCAAATAGTGGTGATGAGGGTAACATCATAATTGATTCTGGTACAACGTTGACAGTTATTCCAACTGGTGTTTATAATAATTTGGAATCAGCTGTGTTGGAATTGGTGAAACTGAAGCGTGTTGATGATCCAACCCGAGAATTTAACCTTTGCTATAGTGTCACATCAGATGGATATGATTTTCCTATAATAACGGCACATTTTAAAGGTGCAGATGTTAAGTTGCATCCTATTAGTTTATTTGTTGCTGTTGCAGATGGCGTTGTTTGCTTTGCTTTTGCAGTTACTTCTGGGTTCCCTGGTTCTGACCTTTCCATCTTTGGAAACTTAGCTCAGCAGAATTTGTTGGTTGGTTATGACCTTCAACAAAAAATTGTGTCGTTTAAGCCTACTGATTGTAGCAAAGTGTAA
Protein sequence

>MS.gene45780.t1

MNTCSLLSLFYFSLCFIISLSRALNNGFSVELIHRDSPKSPLYQPTQNKYQHIVNAARRSRNRANHFYKTALINTPQSTVIPDHGEYLMTYSVGTPPFKLYGIADTGSDIVWLQCEPCKECYNQTTPKFEPSKSSTYKNIPCSSDLCKSVQQGADCNDQNFCEYSIFYGDTSQSKGNLSVDTLTLESSTGRPISFPKTVIGCGTDNTMSFEGASSGVVGLGGGPASLITQLGSSIDSKFSYCLLPNPVESNTTSKLNFGDAAVVSGDGVVSTPIVKKDPIVFYYLTLEAFSVGNKRIEFKGSSNSGDEGNIIIDSGTTLTVIPTGVYNNLESAVLELVKLKRVDDPTREFNLCYSVTSDGYDFPIITAHFKGADVKLHPISLFVAVADGVVCFAFAVTSGFPGSDLSIFGNLAQQNLLVGYDLQQKIVSFKPTDCSKV