AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45781

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45781.t1 MTR_8g038280 91.284 436 38 0 1 436 1 436 0.0 823
MS.gene45781.t1 MTR_8g096990 72.018 436 118 1 1 436 1 432 0.0 653
MS.gene45781.t1 MTR_5g080890 73.288 438 111 4 1 436 1 434 0.0 651
MS.gene45781.t1 MTR_5g080960 72.603 438 114 4 1 436 1 434 0.0 646
MS.gene45781.t1 MTR_5g044770 68.349 436 133 2 1 436 1 431 0.0 614
MS.gene45781.t1 MTR_8g038250 69.457 442 103 3 1 436 1 416 0.0 612
MS.gene45781.t1 MTR_6g090290 65.596 436 149 1 1 435 1 436 0.0 557
MS.gene45781.t1 MTR_8g038310 60.550 436 148 4 1 435 1 413 0.0 526
MS.gene45781.t1 MTR_4g109760 63.073 436 124 5 1 436 1 399 2.13e-175 496
MS.gene45781.t1 MTR_3g028120 51.896 422 191 6 23 436 22 439 5.26e-146 423
MS.gene45781.t1 MTR_5g038360 50.000 440 204 8 6 435 3 436 4.16e-139 405
MS.gene45781.t1 MTR_2g067830 53.771 411 178 8 32 435 1 406 1.39e-138 403
MS.gene45781.t1 MTR_6g088760 52.439 410 186 7 1 404 1 407 2.64e-135 399
MS.gene45781.t1 MTR_5g044570 48.533 443 179 12 5 435 6 411 2.52e-129 380
MS.gene45781.t1 MTR_2g105300 50.735 408 167 9 32 432 1 381 6.67e-125 367
MS.gene45781.t1 MTR_0024s0040 49.161 417 194 8 30 435 1 410 4.58e-124 366
MS.gene45781.t1 MTR_0036s0050 42.760 442 226 10 9 435 6 435 5.11e-109 328
MS.gene45781.t1 MTR_0036s0070 41.648 437 229 10 9 435 6 426 1.31e-106 322
MS.gene45781.t1 MTR_0036s0110 40.590 441 230 10 13 435 16 442 3.94e-98 301
MS.gene45781.t1 MTR_5g008070 40.920 413 221 9 28 434 30 425 1.61e-91 283
MS.gene45781.t1 MTR_5g008110 40.751 373 206 7 70 435 51 415 3.80e-90 280
MS.gene45781.t1 MTR_5g008150 40.260 385 214 8 59 435 36 412 8.51e-90 278
MS.gene45781.t1 MTR_2g059460 43.003 393 199 12 52 435 30 406 1.12e-89 278
MS.gene45781.t1 MTR_2g059490 44.011 359 188 8 86 436 58 411 4.21e-89 278
MS.gene45781.t1 MTR_1g060750 36.855 407 214 11 40 435 2 376 4.22e-75 239
MS.gene45781.t1 MTR_3g097150 35.076 459 258 15 2 436 7 449 1.69e-74 240
MS.gene45781.t1 MTR_4g045857 43.320 247 131 5 74 315 46 288 1.56e-57 191
MS.gene45781.t1 MTR_5g008090 38.079 302 171 8 142 435 21 314 1.68e-57 192
MS.gene45781.t1 MTR_7g117430 34.180 433 233 17 18 433 87 484 6.26e-57 195
MS.gene45781.t1 MTR_1g039270 31.973 441 248 19 22 433 52 469 4.80e-52 182
MS.gene45781.t1 MTR_1g115210 31.265 419 259 11 30 433 82 486 2.16e-51 180
MS.gene45781.t1 MTR_2g028190 32.538 461 268 15 5 433 2 451 3.41e-50 176
MS.gene45781.t1 MTR_1g111350 33.795 361 204 15 85 433 130 467 2.51e-46 166
MS.gene45781.t1 MTR_3g046300 32.474 388 238 11 68 436 79 461 1.50e-45 164
MS.gene45781.t1 MTR_3g053710 32.967 364 216 10 85 433 116 466 2.81e-44 160
MS.gene45781.t1 MTR_4g007740 32.506 403 225 16 55 434 100 478 1.70e-43 159
MS.gene45781.t1 MTR_6g088765 40.081 247 110 4 162 407 5 214 1.11e-42 150
MS.gene45781.t1 MTR_5g091130 30.166 421 263 13 38 436 145 556 5.06e-41 153
MS.gene45781.t1 MTR_6g085010 32.493 357 211 12 86 433 156 491 1.64e-40 151
MS.gene45781.t1 MTR_8g103880 30.056 356 223 12 87 433 117 455 3.61e-39 147
MS.gene45781.t1 MTR_2g070430 28.755 466 277 18 2 430 3 450 4.32e-38 144
MS.gene45781.t1 MTR_2g055840 29.058 382 232 13 78 433 5 373 1.92e-33 129
MS.gene45781.t1 MTR_7g105850 28.717 491 256 22 9 435 7 467 4.46e-30 122
MS.gene45781.t1 MTR_2g063110 26.471 510 285 19 2 433 11 508 1.42e-29 120
MS.gene45781.t1 MTR_3g053980 31.217 378 223 13 73 433 128 485 2.39e-29 120
MS.gene45781.t1 MTR_8g094590 27.344 384 219 15 85 436 72 427 3.50e-29 119
MS.gene45781.t1 MTR_3g065160 28.686 373 223 15 88 436 76 429 1.01e-28 117
MS.gene45781.t1 MTR_3g068000 26.943 386 221 14 89 433 76 441 4.19e-27 113
MS.gene45781.t1 MTR_3g096930 25.743 404 239 15 65 436 63 437 1.12e-26 111
MS.gene45781.t1 MTR_7g080230 25.390 449 269 16 27 434 8 431 2.06e-26 111
MS.gene45781.t1 MTR_8g094590 28.571 364 201 15 85 421 72 403 2.06e-26 110
MS.gene45781.t1 MTR_4g008110 48.333 120 35 1 158 277 45 137 1.10e-25 102
MS.gene45781.t1 MTR_1g078240 26.368 402 251 15 62 434 45 430 1.74e-25 108
MS.gene45781.t1 MTR_1g061940 28.647 377 229 16 85 434 82 445 2.39e-25 108
MS.gene45781.t1 MTR_7g021510 28.238 386 226 20 82 434 67 434 3.14e-25 108
MS.gene45781.t1 MTR_7g062360 26.992 389 221 16 89 436 62 428 4.11e-25 107
MS.gene45781.t1 MTR_4g095270 26.190 420 256 18 41 436 37 426 6.06e-25 108
MS.gene45781.t1 MTR_4g095270 26.190 420 256 18 41 436 37 426 6.42e-25 107
MS.gene45781.t1 MTR_8g094590 31.679 262 146 10 85 337 72 309 1.25e-24 103
MS.gene45781.t1 MTR_1g078240 26.551 403 250 16 62 434 45 431 3.28e-24 105
MS.gene45781.t1 MTR_3g006820 26.456 412 236 17 64 436 32 415 1.68e-23 102
MS.gene45781.t1 MTR_2g084550 27.851 377 231 17 85 434 76 438 4.45e-22 99.0
MS.gene45781.t1 MTR_7g102750 26.525 377 235 17 85 434 80 441 8.07e-22 98.2
MS.gene45781.t1 MTR_0945s0010 40.476 126 71 3 314 436 1 125 1.19e-21 91.7
MS.gene45781.t1 MTR_5g016260 24.146 410 253 17 49 436 61 434 1.28e-21 97.8
MS.gene45781.t1 MTR_5g016260 24.146 410 253 17 49 436 61 434 1.33e-21 97.8
MS.gene45781.t1 MTR_8g039540 28.062 449 279 18 6 434 10 434 1.61e-21 96.7
MS.gene45781.t1 MTR_5g077850 26.738 374 233 13 88 436 83 440 2.13e-21 96.3
MS.gene45781.t1 MTR_4g056420 27.321 377 232 18 85 434 76 437 3.16e-21 96.3
MS.gene45781.t1 MTR_3g014290 28.646 384 227 16 69 434 63 417 3.43e-21 95.5
MS.gene45781.t1 MTR_1g037630 28.646 384 227 16 69 434 75 429 4.13e-21 95.5
MS.gene45781.t1 MTR_1g029810 26.031 388 220 18 85 436 56 412 7.04e-21 94.7
MS.gene45781.t1 MTR_1g029810 26.031 388 220 18 85 436 56 412 7.14e-21 94.7
MS.gene45781.t1 MTR_1g078240 27.083 336 208 13 62 376 45 364 2.10e-20 92.8
MS.gene45781.t1 MTR_6g084710 25.974 385 234 15 80 433 186 550 6.91e-20 92.4
MS.gene45781.t1 MTR_8g075090 27.249 378 217 19 87 435 113 461 7.78e-20 92.0
MS.gene45781.t1 MTR_4g023650 24.631 406 246 17 66 436 42 422 3.59e-19 89.7
MS.gene45781.t1 MTR_5g022880 24.832 447 271 19 28 433 39 461 6.46e-19 89.4
MS.gene45781.t1 MTR_7g102750 27.011 348 213 17 85 406 80 412 2.67e-18 87.0
MS.gene45781.t1 MTR_5g061690 26.027 438 251 21 28 421 25 433 2.85e-18 87.4
MS.gene45781.t1 MTR_6g084710 26.490 302 184 12 80 368 186 462 3.37e-18 87.0
MS.gene45781.t1 MTR_2g103340 38.793 116 27 1 21 136 34 105 3.91e-18 82.4
MS.gene45781.t1 MTR_4g023630 23.267 404 254 14 66 436 42 422 1.08e-17 85.1
MS.gene45781.t1 MTR_5g012490 25.202 496 283 24 1 434 1 470 1.18e-17 85.5
MS.gene45781.t1 MTR_7g080230 25.352 355 214 14 110 434 28 361 2.67e-16 80.9
MS.gene45781.t1 MTR_3g087780 23.851 457 276 21 25 436 24 453 2.89e-16 81.3
MS.gene45781.t1 MTR_5g077920 25.201 373 240 12 88 435 70 428 7.46e-14 73.6
MS.gene45781.t1 MTR_5g061690 27.458 295 180 15 138 421 23 294 4.67e-13 70.9
MS.gene45781.t1 MTR_3g100500 28.409 352 213 16 85 420 96 424 1.79e-12 69.3
MS.gene45781.t1 MTR_1g072380 24.181 397 217 18 86 428 56 422 2.27e-11 65.9
MS.gene45781.t1 MTR_2g037710 27.570 214 125 9 227 433 15 205 5.70e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45781.t1 AT1G64830 48.792 414 205 4 25 436 23 431 1.01e-130 384
MS.gene45781.t1 AT5G33340 50.605 413 194 6 28 436 31 437 7.31e-126 372
MS.gene45781.t1 AT2G35615 44.820 444 220 8 12 434 5 444 2.03e-110 333
MS.gene45781.t1 AT1G31450 45.011 451 222 10 1 434 1 442 1.19e-107 325
MS.gene45781.t1 AT2G28010 38.288 444 216 12 1 436 1 394 5.96e-84 263
MS.gene45781.t1 AT2G28040 38.375 443 216 15 1 436 1 393 3.43e-81 256
MS.gene45781.t1 AT2G28040 41.667 384 192 13 58 436 28 384 3.55e-80 253
MS.gene45781.t1 AT2G28220 36.018 447 228 11 1 436 1 400 1.08e-76 244
MS.gene45781.t1 AT2G28030 41.444 374 188 10 69 436 42 390 2.25e-76 243
MS.gene45781.t1 AT2G03200 37.986 437 229 15 25 436 42 461 5.59e-75 242
MS.gene45781.t1 AT2G28225 35.268 448 230 13 1 436 1 400 8.01e-70 226
MS.gene45781.t1 AT1G01300 36.740 362 199 14 85 433 140 484 5.53e-54 187
MS.gene45781.t1 AT4G30030 35.092 379 211 16 70 433 61 419 7.94e-54 185
MS.gene45781.t1 AT3G61820 35.013 397 219 16 55 433 108 483 1.16e-53 186
MS.gene45781.t1 AT2G23945 28.817 465 271 16 9 436 10 451 3.45e-51 179
MS.gene45781.t1 AT3G18490 30.602 415 243 14 28 433 122 500 3.83e-50 177
MS.gene45781.t1 AT3G20015 28.864 440 267 16 20 433 51 470 3.86e-46 166
MS.gene45781.t1 AT5G10770 33.511 376 216 12 77 434 115 474 2.66e-45 164
MS.gene45781.t1 AT1G25510 34.722 360 202 14 85 433 146 483 5.82e-45 163
MS.gene45781.t1 AT5G10760 31.873 411 239 15 31 433 87 464 8.59e-42 154
MS.gene45781.t1 AT4G30040 30.023 443 262 19 9 428 10 427 3.26e-41 152
MS.gene45781.t1 AT3G25700 29.535 430 251 18 39 434 39 450 3.83e-41 152
MS.gene45781.t1 AT3G12700 28.698 453 287 17 3 434 24 461 9.37e-40 148
MS.gene45781.t1 AT1G79720 32.609 368 207 12 87 433 135 482 1.78e-38 145
MS.gene45781.t1 AT3G59080 31.099 373 231 14 85 436 168 535 2.17e-38 146
MS.gene45781.t1 AT2G42980 31.565 377 226 14 85 436 158 527 3.33e-38 145
MS.gene45781.t1 AT5G36260 29.396 381 216 15 85 434 76 434 7.21e-36 138
MS.gene45781.t1 AT2G39710 28.496 379 212 16 89 428 67 425 1.53e-30 122
MS.gene45781.t1 AT3G59080 30.163 368 205 13 85 436 168 499 8.26e-30 121
MS.gene45781.t1 AT3G54400 29.188 394 234 14 62 434 56 425 9.58e-30 120
MS.gene45781.t1 AT3G02740 28.982 383 214 17 85 434 83 440 2.25e-29 120
MS.gene45781.t1 AT3G02740 28.609 381 214 17 87 434 10 365 5.13e-29 118
MS.gene45781.t1 AT4G12920 26.478 389 238 14 61 433 31 387 1.14e-27 114
MS.gene45781.t1 AT1G09750 27.979 386 235 14 72 433 82 448 2.15e-27 114
MS.gene45781.t1 AT5G37540 26.882 372 232 13 88 433 81 438 3.30e-27 113
MS.gene45781.t1 AT4G33490 26.166 386 222 15 85 436 58 414 7.64e-27 112
MS.gene45781.t1 AT1G66180 26.613 372 235 14 88 436 73 429 8.24e-27 112
MS.gene45781.t1 AT1G65240 26.053 380 227 16 85 434 72 427 5.38e-26 110
MS.gene45781.t1 AT5G02190 27.441 379 216 18 96 433 82 442 2.78e-25 107
MS.gene45781.t1 AT2G36670 26.471 374 238 15 85 434 98 458 5.36e-24 104
MS.gene45781.t1 AT2G36670 26.344 372 237 15 87 434 34 392 1.17e-23 103
MS.gene45781.t1 AT2G36670 26.344 372 237 15 87 434 34 392 1.17e-23 103
MS.gene45781.t1 AT2G36670 26.344 372 237 15 87 434 105 463 1.42e-23 103
MS.gene45781.t1 AT3G50050 26.161 409 251 17 55 436 55 439 3.12e-23 102
MS.gene45781.t1 AT5G43100 27.321 377 219 18 85 436 74 420 3.25e-23 102
MS.gene45781.t1 AT1G05840 27.604 384 229 18 82 434 74 439 4.37e-23 101
MS.gene45781.t1 AT5G43100 27.321 377 219 18 85 436 74 420 6.58e-23 102
MS.gene45781.t1 AT1G77480 24.675 385 230 16 85 436 65 422 7.03e-23 100
MS.gene45781.t1 AT1G44130 25.926 405 238 16 68 436 24 402 8.58e-23 100
MS.gene45781.t1 AT2G17760 25.995 427 246 20 45 433 55 449 1.20e-22 100
MS.gene45781.t1 AT1G77480 24.675 385 230 16 85 436 65 422 1.31e-22 100
MS.gene45781.t1 AT4G33490 28.664 307 173 13 85 374 55 332 1.60e-22 99.4
MS.gene45781.t1 AT3G52500 29.310 406 206 21 85 434 88 468 1.06e-21 97.4
MS.gene45781.t1 AT5G22850 26.790 377 235 17 85 434 79 441 7.88e-21 95.1
MS.gene45781.t1 AT3G51350 25.556 450 273 18 23 433 26 452 4.43e-20 93.2
MS.gene45781.t1 AT1G08210 28.460 383 218 18 85 434 116 475 1.27e-19 91.7
MS.gene45781.t1 AT1G08210 28.460 383 218 18 85 434 130 489 1.41e-19 91.7
MS.gene45781.t1 AT1G08210 28.460 383 218 18 85 434 82 441 1.55e-19 91.3
MS.gene45781.t1 AT1G49050 24.619 394 239 17 80 435 197 570 1.11e-18 89.0
MS.gene45781.t1 AT1G49050 23.757 362 231 15 105 435 50 397 1.40e-18 87.8
MS.gene45781.t1 AT5G07030 27.966 354 210 15 87 421 115 442 3.78e-18 86.7
MS.gene45781.t1 AT3G51330 25.393 445 271 20 28 433 31 453 1.02e-17 85.5
MS.gene45781.t1 AT3G51330 25.393 445 271 20 28 433 31 453 1.42e-17 85.5
MS.gene45781.t1 AT1G08210 26.829 410 217 19 85 434 82 468 7.14e-16 80.1
MS.gene45781.t1 AT3G42550 22.427 379 223 12 74 434 65 390 1.65e-15 78.6
MS.gene45781.t1 AT3G25700 27.684 177 107 6 39 202 39 207 1.71e-15 77.8
MS.gene45781.t1 AT3G51340 22.955 379 228 13 92 433 108 459 3.07e-15 78.2
MS.gene45781.t1 AT5G10080 25.104 482 271 27 11 435 7 455 5.04e-15 77.4
MS.gene45781.t1 AT1G49050 26.838 272 168 11 80 337 197 451 1.08e-13 73.2
MS.gene45781.t1 AT3G51360 25.405 370 220 18 92 433 104 445 9.37e-13 70.5
MS.gene45781.t1 AT3G51360 25.405 370 220 18 92 433 94 435 1.18e-12 70.1
MS.gene45781.t1 AT3G51360 25.405 370 220 18 92 433 10 351 1.74e-12 69.3
MS.gene45781.t1 AT4G35880 22.622 473 289 20 8 433 9 451 2.96e-12 68.9

Find 88 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACGCCACCTTTGGAAATT+TGG 0.149363 8.3:-58589814 MS.gene45781:CDS
GTCAAATAGTAATAGGTTTC+AGG 0.170628 8.3:+58590179 None:intergenic
TGTGAATATTCTACTTATTA+TGG 0.199216 8.3:-58590542 MS.gene45781:CDS
ATACCGGTAGTGATATTATT+TGG 0.208017 8.3:-58590709 MS.gene45781:CDS
TTAAAATGTGTGGTGATTAT+AGG 0.215291 8.3:+58589924 None:intergenic
GGTACAATCAGTAGGTTTAA+AGG 0.233685 8.3:+58589743 None:intergenic
CCACATCCTATCACAATATT+AGG 0.259740 8.3:+58590440 None:intergenic
GCAATAGCATATAACTTAAA+TGG 0.279990 8.3:+58590734 None:intergenic
AACAAATTGAAATGAATAGA+AGG 0.315287 8.3:+58590999 None:intergenic
GGAGATGCGGCTATGGTATC+TGG 0.323023 8.3:-58590239 MS.gene45781:CDS
GGAAATAAAAGAGTAGAAAT+TGG 0.326779 8.3:-58590137 MS.gene45781:CDS
TCCTATTAGCACATTTGTTC+CGG 0.333439 8.3:-58589880 MS.gene45781:CDS
TCATTTCCTAATATTGTGAT+AGG 0.341630 8.3:-58590446 MS.gene45781:CDS
TGCTGATGGTGTTGTTTGCT+TGG 0.342994 8.3:-58589856 MS.gene45781:CDS
TTCCAATGTCAAATAGTAAT+AGG 0.343339 8.3:+58590172 None:intergenic
ATGAGATACTCGCCTTTATC+AGG 0.348879 8.3:+58590779 None:intergenic
GGTAACATCATAATTGATTC+AGG 0.364855 8.3:-58590089 MS.gene45781:CDS
GGAATCTGCTGTGGTAGATT+TGG 0.372955 8.3:-58590024 MS.gene45781:CDS
CGGCTATGGTATCTGGCAAC+GGG 0.373663 8.3:-58590232 MS.gene45781:CDS
ATAGATTGACACAACTTTGA+TGG 0.374549 8.3:+58590596 None:intergenic
TCTCAAGATGACGCCACCTT+TGG 0.374689 8.3:-58589822 MS.gene45781:CDS
TCAACTGTAATACCTGATAA+AGG 0.391550 8.3:-58590791 MS.gene45781:CDS
AAACAATGGTGTTAAACAAT+AGG 0.394363 8.3:+58590307 None:intergenic
GTGCAGCATTGACAAAATAT+TGG 0.403113 8.3:+58590873 None:intergenic
GAAATGATTGGCACGGTTGA+TGG 0.407103 8.3:+58590844 None:intergenic
TTTGGCTCAACAAAATTTGT+TGG 0.408329 8.3:-58589796 MS.gene45781:CDS
CCTAATATTGTGATAGGATG+TGG 0.415797 8.3:-58590440 MS.gene45781:CDS
GGCATAGTTGGCCTTGGAGG+TGG 0.421810 8.3:-58590380 MS.gene45781:CDS
CAAACAACACCATCAGCAAC+CGG 0.424779 8.3:+58589861 None:intergenic
GGCATAAGCTCTGGCATAGT+TGG 0.426354 8.3:-58590392 MS.gene45781:CDS
ACTCAATTGGGATCCTCAAC+TGG 0.427072 8.3:-58590341 MS.gene45781:CDS
AGCTCTGGCATAGTTGGCCT+TGG 0.428693 8.3:-58590386 MS.gene45781:CDS
ACCGGAACAAATGTGCTAAT+AGG 0.436739 8.3:+58589879 None:intergenic
TCTGGCATAGTTGGCCTTGG+AGG 0.436740 8.3:-58590383 MS.gene45781:CDS
TGGTGGGGTACCAACTGAAT+AGG 0.458426 8.3:+58590754 None:intergenic
TCAAATAGTAATAGGTTTCA+GGG 0.463391 8.3:+58590180 None:intergenic
GCAACGAGCAAGCTCAACTT+TGG 0.464327 8.3:-58590260 MS.gene45781:CDS
GCTCAACAAAATTTGTTGGT+TGG 0.467228 8.3:-58589792 MS.gene45781:CDS
TAGTGTTGATACTCTTACAT+TGG 0.467324 8.3:-58590492 MS.gene45781:CDS
TAGATTGACACAACTTTGAT+GGG 0.480149 8.3:+58590597 None:intergenic
AACAATATTATGTCTTATGA+AGG 0.482827 8.3:-58590413 MS.gene45781:CDS
CAACTTTGGAGATGCGGCTA+TGG 0.494629 8.3:-58590246 MS.gene45781:CDS
TCAGGTATTACAGTTGATTG+AGG 0.502820 8.3:+58590797 None:intergenic
TTGATTGAGGTATATTTGCA+AGG 0.505434 8.3:+58590810 None:intergenic
TAGCATATAACTTAAATGGT+GGG 0.516419 8.3:+58590738 None:intergenic
CAAAGGCTTAATGTTTGAGT+AGG 0.527299 8.3:+58589978 None:intergenic
ACATTGGAAGCATTTAGTGT+TGG 0.529902 8.3:-58590158 MS.gene45781:CDS
TTATATGCTATTGCTGATAC+CGG 0.532039 8.3:-58590725 MS.gene45781:CDS
GAAATTGGAGGAGTTGCAAA+TGG 0.536528 8.3:-58590122 MS.gene45781:CDS
TGAAACTGTAAGTCCATTGG+TGG 0.538274 8.3:+58590466 None:intergenic
TTGTAACTTGACGATTTGGA+CGG 0.538622 8.3:+58590632 None:intergenic
TCTTATGAAGGCATAAGCTC+TGG 0.539135 8.3:-58590401 MS.gene45781:CDS
ACATTTGTTCCGGTTGCTGA+TGG 0.540335 8.3:-58589870 MS.gene45781:CDS
ACAAATTGAAATGAATAGAA+GGG 0.543707 8.3:+58591000 None:intergenic
GGAGAATTTACCACCAGTTG+AGG 0.544348 8.3:+58590328 None:intergenic
TGACACAACTTTGATGGGCA+AGG 0.547878 8.3:+58590602 None:intergenic
AATAAAAGAGTAGAAATTGG+AGG 0.549005 8.3:-58590134 MS.gene45781:CDS
TGCTATAGTGTCAAAGCTGA+GGG 0.551512 8.3:-58589957 MS.gene45781:CDS
TTGAGCCAAATTTCCAAAGG+TGG 0.552185 8.3:+58589809 None:intergenic
GCGGCTATGGTATCTGGCAA+CGG 0.563430 8.3:-58590233 MS.gene45781:CDS
AAATGAAACTGTAAGTCCAT+TGG 0.564165 8.3:+58590463 None:intergenic
AAGCCAAATAATATCACTAC+CGG 0.565433 8.3:+58590706 None:intergenic
TGATTGAGGTATATTTGCAA+GGG 0.566811 8.3:+58590811 None:intergenic
AACCTATTACTATTTGACAT+TGG 0.569683 8.3:-58590174 MS.gene45781:CDS
GGTGATAATTCACATTCAGA+AGG 0.574167 8.3:-58590521 MS.gene45781:CDS
CACACTTTGGTACAATCAGT+AGG 0.575657 8.3:+58589735 None:intergenic
CAGCTTTGACACTATAGCAA+AGG 0.579289 8.3:+58589961 None:intergenic
AGCATATAACTTAAATGGTG+GGG 0.581392 8.3:+58590739 None:intergenic
CTATAAAAGTGATCACACTT+TGG 0.586982 8.3:+58589722 None:intergenic
AAGATGCGGGTCCACCTCCA+AGG 0.595636 8.3:+58590369 None:intergenic
ATAGCATATAACTTAAATGG+TGG 0.597247 8.3:+58590737 None:intergenic
GATTTGGACGGATTAAACAT+AGG 0.598664 8.3:+58590644 None:intergenic
TTGTTGAGCCAAATTTCCAA+AGG 0.603204 8.3:+58589806 None:intergenic
TTGCTATAGTGTCAAAGCTG+AGG 0.607476 8.3:-58589958 MS.gene45781:CDS
TCGTTGTAACATTGTTGACA+AGG 0.608209 8.3:+58590674 None:intergenic
GGACGGATTAAACATAGGAG+TGG 0.612846 8.3:+58590649 None:intergenic
AATTGAGTGATAAAAGATGC+GGG 0.617632 8.3:+58590356 None:intergenic
ATGTTTGTTACAGAAAACAA+TGG 0.624952 8.3:+58590293 None:intergenic
AGTTGCAAATGGTGATGATG+AGG 0.627898 8.3:-58590111 MS.gene45781:CDS
TATCTCATGACCTATTCAGT+TGG 0.628251 8.3:-58590764 MS.gene45781:CDS
CTTACATTGGAATCCACCAA+TGG 0.633185 8.3:-58590479 MS.gene45781:CDS
GTTGCAAATGGTGATGATGA+GGG 0.638026 8.3:-58590110 MS.gene45781:CDS
CAATTGAGTGATAAAAGATG+CGG 0.639675 8.3:+58590355 None:intergenic
ATCTGCACCTTTAAAATGTG+TGG 0.648432 8.3:+58589914 None:intergenic
CAAGCTCAACTTTGGAGATG+CGG 0.650651 8.3:-58590252 MS.gene45781:CDS
GGTGGCGTCATCTTGAGATG+AGG 0.659524 8.3:+58589827 None:intergenic
CAATTGGGATCCTCAACTGG+TGG 0.671748 8.3:-58590338 MS.gene45781:CDS
GGCTATGGTATCTGGCAACG+GGG 0.692106 8.3:-58590231 MS.gene45781:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAATTGAAATGAATAGA+AGG + chr8.3:58589760-58589779 None:intergenic 20.0%
!! AACAATATTATGTCTTATGA+AGG - chr8.3:58590343-58590362 MS.gene45781:CDS 20.0%
!! ACAAATTGAAATGAATAGAA+GGG + chr8.3:58589759-58589778 None:intergenic 20.0%
!! TGTGAATATTCTACTTATTA+TGG - chr8.3:58590214-58590233 MS.gene45781:CDS 20.0%
!!! AAAAGATGATTACTCTTTTT+TGG - chr8.3:58590711-58590730 MS.gene45781:CDS 20.0%
! AACCTATTACTATTTGACAT+TGG - chr8.3:58590582-58590601 MS.gene45781:CDS 25.0%
! AATAAAAGAGTAGAAATTGG+AGG - chr8.3:58590622-58590641 MS.gene45781:CDS 25.0%
! ATAGCATATAACTTAAATGG+TGG + chr8.3:58590022-58590041 None:intergenic 25.0%
! GCAATAGCATATAACTTAAA+TGG + chr8.3:58590025-58590044 None:intergenic 25.0%
! GGAAATAAAAGAGTAGAAAT+TGG - chr8.3:58590619-58590638 MS.gene45781:CDS 25.0%
! TAGCATATAACTTAAATGGT+GGG + chr8.3:58590021-58590040 None:intergenic 25.0%
! TCAAATAGTAATAGGTTTCA+GGG + chr8.3:58590579-58590598 None:intergenic 25.0%
! TCATTTCCTAATATTGTGAT+AGG - chr8.3:58590310-58590329 MS.gene45781:CDS 25.0%
! TTAAAATGTGTGGTGATTAT+AGG + chr8.3:58590835-58590854 None:intergenic 25.0%
! TTCCAATGTCAAATAGTAAT+AGG + chr8.3:58590587-58590606 None:intergenic 25.0%
!! AAACAATGGTGTTAAACAAT+AGG + chr8.3:58590452-58590471 None:intergenic 25.0%
!! ATAATCACCACACATTTTAA+AGG - chr8.3:58590835-58590854 MS.gene45781:CDS 25.0%
!! ATGTTTGTTACAGAAAACAA+TGG + chr8.3:58590466-58590485 None:intergenic 25.0%
!!! ATAAGTTTTGTCATTACATG+AGG + chr8.3:58590194-58590213 None:intergenic 25.0%
!!! TATTGGTATTTGTTTTGAGT+AGG + chr8.3:58589869-58589888 None:intergenic 25.0%
AAATGAAACTGTAAGTCCAT+TGG + chr8.3:58590296-58590315 None:intergenic 30.0%
AAGCCAAATAATATCACTAC+CGG + chr8.3:58590053-58590072 None:intergenic 30.0%
AATTGAGTGATAAAAGATGC+GGG + chr8.3:58590403-58590422 None:intergenic 30.0%
AGCATATAACTTAAATGGTG+GGG + chr8.3:58590020-58590039 None:intergenic 30.0%
ATACCGGTAGTGATATTATT+TGG - chr8.3:58590047-58590066 MS.gene45781:CDS 30.0%
ATAGATTGACACAACTTTGA+TGG + chr8.3:58590163-58590182 None:intergenic 30.0%
CAATTGAGTGATAAAAGATG+CGG + chr8.3:58590404-58590423 None:intergenic 30.0%
GTCAAATAGTAATAGGTTTC+AGG + chr8.3:58590580-58590599 None:intergenic 30.0%
TAGATTGACACAACTTTGAT+GGG + chr8.3:58590162-58590181 None:intergenic 30.0%
TCAACTGTAATACCTGATAA+AGG - chr8.3:58589965-58589984 MS.gene45781:CDS 30.0%
TGATTGAGGTATATTTGCAA+GGG + chr8.3:58589948-58589967 None:intergenic 30.0%
TTATATGCTATTGCTGATAC+CGG - chr8.3:58590031-58590050 MS.gene45781:CDS 30.0%
TTGATTGAGGTATATTTGCA+AGG + chr8.3:58589949-58589968 None:intergenic 30.0%
! GCATCTTTTATCACTCAATT+GGG - chr8.3:58590403-58590422 MS.gene45781:CDS 30.0%
! GGAGTTTTTGTAGAAATGAT+TGG + chr8.3:58589927-58589946 None:intergenic 30.0%
! GGTAACATCATAATTGATTC+AGG - chr8.3:58590667-58590686 MS.gene45781:CDS 30.0%
! GTTTTTGTAACTTGACGATT+TGG + chr8.3:58590131-58590150 None:intergenic 30.0%
! TTTGGCTCAACAAAATTTGT+TGG - chr8.3:58590960-58590979 MS.gene45781:CDS 30.0%
! TTTTGTAGAAATGATTGGCA+CGG + chr8.3:58589922-58589941 None:intergenic 30.0%
!! TAGTGTTGATACTCTTACAT+TGG - chr8.3:58590264-58590283 MS.gene45781:CDS 30.0%
!! TTTTGAGTAGGTTTATAGAG+TGG + chr8.3:58589857-58589876 None:intergenic 30.0%
!!! TCAGGGTCTTTTTTCAATAT+AGG + chr8.3:58590562-58590581 None:intergenic 30.0%
CAAAGGCTTAATGTTTGAGT+AGG + chr8.3:58590781-58590800 None:intergenic 35.0%
CCACATCCTATCACAATATT+AGG + chr8.3:58590319-58590338 None:intergenic 35.0%
CCTAATATTGTGATAGGATG+TGG - chr8.3:58590316-58590335 MS.gene45781:CDS 35.0%
GGTACAATCAGTAGGTTTAA+AGG + chr8.3:58591016-58591035 None:intergenic 35.0%
GGTGATAATTCACATTCAGA+AGG - chr8.3:58590235-58590254 MS.gene45781:CDS 35.0%
TATCTCATGACCTATTCAGT+TGG - chr8.3:58589992-58590011 MS.gene45781:CDS 35.0%
TCAGGTATTACAGTTGATTG+AGG + chr8.3:58589962-58589981 None:intergenic 35.0%
TCCTATTAGCACATTTGTTC+CGG - chr8.3:58590876-58590895 MS.gene45781:CDS 35.0%
TCGTTGTAACATTGTTGACA+AGG + chr8.3:58590085-58590104 None:intergenic 35.0%
TTGTTGAGCCAAATTTCCAA+AGG + chr8.3:58590953-58590972 None:intergenic 35.0%
! CGCATCTTTTATCACTCAAT+TGG - chr8.3:58590402-58590421 MS.gene45781:CDS 35.0%
! TTGTAACTTGACGATTTGGA+CGG + chr8.3:58590127-58590146 None:intergenic 35.0%
!! ACATTGGAAGCATTTAGTGT+TGG - chr8.3:58590598-58590617 MS.gene45781:CDS 35.0%
!! ATCTGCACCTTTAAAATGTG+TGG + chr8.3:58590845-58590864 None:intergenic 35.0%
!! GATTTGGACGGATTAAACAT+AGG + chr8.3:58590115-58590134 None:intergenic 35.0%
!! GCTCAACAAAATTTGTTGGT+TGG - chr8.3:58590964-58590983 MS.gene45781:CDS 35.0%
!! GTGCAGCATTGACAAAATAT+TGG + chr8.3:58589886-58589905 None:intergenic 35.0%
ACCGGAACAAATGTGCTAAT+AGG + chr8.3:58590880-58590899 None:intergenic 40.0%
ATGAGATACTCGCCTTTATC+AGG + chr8.3:58589980-58589999 None:intergenic 40.0%
CACACTTTGGTACAATCAGT+AGG + chr8.3:58591024-58591043 None:intergenic 40.0%
CTTACATTGGAATCCACCAA+TGG - chr8.3:58590277-58590296 MS.gene45781:CDS 40.0%
GAAATTGGAGGAGTTGCAAA+TGG - chr8.3:58590634-58590653 MS.gene45781:CDS 40.0%
TGAAACTGTAAGTCCATTGG+TGG + chr8.3:58590293-58590312 None:intergenic 40.0%
TGCTATAGTGTCAAAGCTGA+GGG - chr8.3:58590799-58590818 MS.gene45781:CDS 40.0%
TTGAGCCAAATTTCCAAAGG+TGG + chr8.3:58590950-58590969 None:intergenic 40.0%
TTGCTATAGTGTCAAAGCTG+AGG - chr8.3:58590798-58590817 MS.gene45781:CDS 40.0%
! AGTTGCAAATGGTGATGATG+AGG - chr8.3:58590645-58590664 MS.gene45781:CDS 40.0%
! CAGCTTTGACACTATAGCAA+AGG + chr8.3:58590798-58590817 None:intergenic 40.0%
! GTTGCAAATGGTGATGATGA+GGG - chr8.3:58590646-58590665 MS.gene45781:CDS 40.0%
! TCTTATGAAGGCATAAGCTC+TGG - chr8.3:58590355-58590374 MS.gene45781:CDS 40.0%
!! GTGGTGATTTTAAAGAGTCG+CGG + chr8.3:58589838-58589857 None:intergenic 40.0%
!!! CTCTTTTTTGGAATCTGCTG+TGG - chr8.3:58590723-58590742 MS.gene45781:CDS 40.0%
ACATTTGTTCCGGTTGCTGA+TGG - chr8.3:58590886-58590905 MS.gene45781:CDS 45.0%
ACTCAATTGGGATCCTCAAC+TGG - chr8.3:58590415-58590434 MS.gene45781:CDS 45.0%
CAAACAACACCATCAGCAAC+CGG + chr8.3:58590898-58590917 None:intergenic 45.0%
GAAATGATTGGCACGGTTGA+TGG + chr8.3:58589915-58589934 None:intergenic 45.0%
GGAATCTGCTGTGGTAGATT+TGG - chr8.3:58590732-58590751 MS.gene45781:CDS 45.0%
GGAGAATTTACCACCAGTTG+AGG + chr8.3:58590431-58590450 None:intergenic 45.0%
! CAAGCTCAACTTTGGAGATG+CGG - chr8.3:58590504-58590523 MS.gene45781:CDS 45.0%
! TGACACAACTTTGATGGGCA+AGG + chr8.3:58590157-58590176 None:intergenic 45.0%
! TGACGCCACCTTTGGAAATT+TGG - chr8.3:58590942-58590961 MS.gene45781:CDS 45.0%
!! GGACGGATTAAACATAGGAG+TGG + chr8.3:58590110-58590129 None:intergenic 45.0%
!! GTGATTTTAAAGAGTCGCGG+TGG + chr8.3:58589835-58589854 None:intergenic 45.0%
!! TGCTGATGGTGTTGTTTGCT+TGG - chr8.3:58590900-58590919 MS.gene45781:CDS 45.0%
CAATTGGGATCCTCAACTGG+TGG - chr8.3:58590418-58590437 MS.gene45781:CDS 50.0%
GCAACGAGCAAGCTCAACTT+TGG - chr8.3:58590496-58590515 MS.gene45781:CDS 50.0%
TCTCAAGATGACGCCACCTT+TGG - chr8.3:58590934-58590953 MS.gene45781:CDS 50.0%
! CAACTTTGGAGATGCGGCTA+TGG - chr8.3:58590510-58590529 MS.gene45781:CDS 50.0%
! GGCATAAGCTCTGGCATAGT+TGG - chr8.3:58590364-58590383 MS.gene45781:CDS 50.0%
! TGGTGGGGTACCAACTGAAT+AGG + chr8.3:58590005-58590024 None:intergenic 50.0%
CGGCTATGGTATCTGGCAAC+GGG - chr8.3:58590524-58590543 MS.gene45781:CDS 55.0%
GCGGCTATGGTATCTGGCAA+CGG - chr8.3:58590523-58590542 MS.gene45781:CDS 55.0%
GGAGATGCGGCTATGGTATC+TGG - chr8.3:58590517-58590536 MS.gene45781:CDS 55.0%
GGCTATGGTATCTGGCAACG+GGG - chr8.3:58590525-58590544 MS.gene45781:CDS 55.0%
GGTGGCGTCATCTTGAGATG+AGG + chr8.3:58590932-58590951 None:intergenic 55.0%
! AGCTCTGGCATAGTTGGCCT+TGG - chr8.3:58590370-58590389 MS.gene45781:CDS 55.0%
!! TCTGGCATAGTTGGCCTTGG+AGG - chr8.3:58590373-58590392 MS.gene45781:CDS 55.0%
AAGATGCGGGTCCACCTCCA+AGG + chr8.3:58590390-58590409 None:intergenic 60.0%
!! GGCATAGTTGGCCTTGGAGG+TGG - chr8.3:58590376-58590395 MS.gene45781:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 58589734 58591044 58589734 ID=MS.gene45781
chr8.3 mRNA 58589734 58591044 58589734 ID=MS.gene45781.t1;Parent=MS.gene45781
chr8.3 exon 58589734 58591044 58589734 ID=MS.gene45781.t1.exon1;Parent=MS.gene45781.t1
chr8.3 CDS 58589734 58591044 58589734 ID=cds.MS.gene45781.t1;Parent=MS.gene45781.t1
Gene Sequence

>MS.gene45781

ATGAGTAAACGTTCATTTCTTACCCTTCTATTCATTTCAATTTGTTTCATTATTTCTTTTTCTCATACTCAAAAGAATGTTTTTAGTGTTGAACTCATCCACCGCGACTCTTTAAAATCACCACTCTATAAACCTACTCAAAACAAATACCAATATTTTGTCAATGCTGCACGTCGTTCCATCAACCGTGCCAATCATTTCTACAAAAACTCCCTTGCAAATATACCTCAATCAACTGTAATACCTGATAAAGGCGAGTATCTCATGACCTATTCAGTTGGTACCCCACCATTTAAGTTATATGCTATTGCTGATACCGGTAGTGATATTATTTGGCTTCAATGTGAGCCTTGTCAACAATGTTACAACGAAACCACTCCTATGTTTAATCCGTCCAAATCGTCAAGTTACAAAAACATTCCTTGCCCATCAAAGTTGTGTCAATCTATGAAAGATACCTCATGTAATGACAAAACTTATTGTGAATATTCTACTTATTATGGTGATAATTCACATTCAGAAGGAGATCTTAGTGTTGATACTCTTACATTGGAATCCACCAATGGACTTACAGTTTCATTTCCTAATATTGTGATAGGATGTGGAACTAACAATATTATGTCTTATGAAGGCATAAGCTCTGGCATAGTTGGCCTTGGAGGTGGACCCGCATCTTTTATCACTCAATTGGGATCCTCAACTGGTGGTAAATTCTCCTATTGTTTAACACCATTGTTTTCTGTAACAAACATTGAGTCAAACGCAACGAGCAAGCTCAACTTTGGAGATGCGGCTATGGTATCTGGCAACGGGGTTGTGACAACTCCTATATTGAAAAAAGACCCTGAAACCTATTACTATTTGACATTGGAAGCATTTAGTGTTGGAAATAAAAGAGTAGAAATTGGAGGAGTTGCAAATGGTGATGATGAGGGTAACATCATAATTGATTCAGGTACAACATTGACAACTTTAACAAAAGATGATTACTCTTTTTTGGAATCTGCTGTGGTAGATTTGGTGACGTTAGAACGTGTTGACGATCCTACTCAAACATTAAGCCTTTGCTATAGTGTCAAAGCTGAGGGATATGATTTTCCTATAATCACCACACATTTTAAAGGTGCAGATGTCGATTTGCATCCTATTAGCACATTTGTTCCGGTTGCTGATGGTGTTGTTTGCTTGGCTTTTGCCTCATCTCAAGATGACGCCACCTTTGGAAATTTGGCTCAACAAAATTTGTTGGTTGGTTATGATCTTCAGCAAAAAATTGTGTCCTTTAAACCTACTGATTGTACCAAAGTGTGA
Protein sequence

>MS.gene45781.t1

MSKRSFLTLLFISICFIISFSHTQKNVFSVELIHRDSLKSPLYKPTQNKYQYFVNAARRSINRANHFYKNSLANIPQSTVIPDKGEYLMTYSVGTPPFKLYAIADTGSDIIWLQCEPCQQCYNETTPMFNPSKSSSYKNIPCPSKLCQSMKDTSCNDKTYCEYSTYYGDNSHSEGDLSVDTLTLESTNGLTVSFPNIVIGCGTNNIMSYEGISSGIVGLGGGPASFITQLGSSTGGKFSYCLTPLFSVTNIESNATSKLNFGDAAMVSGNGVVTTPILKKDPETYYYLTLEAFSVGNKRVEIGGVANGDDEGNIIIDSGTTLTTLTKDDYSFLESAVVDLVTLERVDDPTQTLSLCYSVKAEGYDFPIITTHFKGADVDLHPISTFVPVADGVVCLAFASSQDDATFGNLAQQNLLVGYDLQQKIVSFKPTDCTKV