AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45855

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45855.t1 MTR_4g025830 92.683 164 9 1 1 164 1 161 3.60e-110 310
MS.gene45855.t1 MTR_0388s0020 51.333 150 62 3 3 152 7 145 6.55e-46 147
MS.gene45855.t1 MTR_7g118010 53.571 84 35 2 82 165 23 102 5.76e-23 87.8
MS.gene45855.t1 MTR_3g084250 55.882 68 26 2 82 148 25 89 4.60e-21 82.4
MS.gene45855.t1 MTR_3g092220 50.000 84 37 2 82 165 33 111 3.83e-20 80.9
MS.gene45855.t1 MTR_8g026730 51.190 84 36 2 82 165 27 105 1.07e-19 79.3
MS.gene45855.t1 MTR_3g084210 57.377 61 22 2 82 141 25 82 9.92e-19 76.6
MS.gene45855.t1 MTR_3g084230 57.377 61 22 2 82 141 25 82 9.92e-19 76.6
MS.gene45855.t1 MTR_4g072220 50.000 72 32 2 79 149 23 91 1.06e-18 76.6
MS.gene45855.t1 MTR_4g072240 50.704 71 31 2 79 148 23 90 1.55e-18 75.9
MS.gene45855.t1 MTR_3g084160 57.377 61 22 2 82 141 25 82 1.64e-18 75.9
MS.gene45855.t1 MTR_4g072300 50.704 71 31 2 79 148 23 90 3.19e-18 75.1
MS.gene45855.t1 MTR_8g461420 55.738 61 23 2 82 141 25 82 6.96e-18 74.3
MS.gene45855.t1 MTR_3g084190 55.738 61 23 2 82 141 25 82 1.31e-17 73.6
MS.gene45855.t1 MTR_3g110045 55.738 61 23 2 82 141 84 141 3.31e-17 74.3
MS.gene45855.t1 MTR_4g072570 48.529 68 31 2 82 148 25 89 3.97e-17 72.4
MS.gene45855.t1 MTR_4g072980 45.333 75 38 2 77 150 22 94 1.14e-16 71.2
MS.gene45855.t1 MTR_4g072850 48.529 68 31 2 82 148 27 91 1.22e-16 71.2
MS.gene45855.t1 MTR_4g072500 50.000 66 29 2 82 146 25 87 2.10e-16 70.5
MS.gene45855.t1 MTR_4g072330 47.059 68 32 2 82 148 23 87 2.58e-16 70.1
MS.gene45855.t1 MTR_3g117640 48.571 70 33 2 82 150 34 101 2.79e-16 70.5
MS.gene45855.t1 MTR_4g072530 51.515 66 28 2 82 146 25 87 3.48e-16 70.1
MS.gene45855.t1 MTR_4g072910 48.529 68 31 2 82 148 35 99 3.80e-16 70.1
MS.gene45855.t1 MTR_4g072230 46.269 67 30 2 82 147 26 87 4.15e-16 69.7
MS.gene45855.t1 MTR_4g072890 47.887 71 33 2 79 148 23 90 4.77e-16 69.7
MS.gene45855.t1 MTR_4g072830 41.772 79 42 2 71 148 12 87 6.41e-16 69.3
MS.gene45855.t1 MTR_4g072250 45.588 68 32 2 82 148 26 89 6.47e-16 69.3
MS.gene45855.t1 MTR_4g072640 48.485 66 30 2 82 146 25 87 8.46e-16 68.9
MS.gene45855.t1 MTR_4g072260 45.833 72 35 2 79 149 23 91 1.18e-15 68.6
MS.gene45855.t1 MTR_4g072820 47.059 68 32 2 82 148 20 84 1.77e-15 68.2
MS.gene45855.t1 MTR_4g072310 45.588 68 33 2 82 148 26 90 2.13e-15 68.2
MS.gene45855.t1 MTR_4g072370 45.070 71 35 2 79 148 20 87 2.25e-15 67.8
MS.gene45855.t1 MTR_4g072870 46.479 71 34 2 79 148 23 90 2.31e-15 67.8
MS.gene45855.t1 MTR_3g117610 50.000 60 27 1 82 141 45 101 2.59e-15 68.2
MS.gene45855.t1 MTR_4g072880 47.059 68 32 2 82 148 26 90 2.82e-15 67.8
MS.gene45855.t1 MTR_4g072160 50.794 63 28 1 82 144 37 96 3.15e-15 68.9
MS.gene45855.t1 MTR_4g072190 44.776 67 34 1 82 148 36 99 3.91e-15 67.4
MS.gene45855.t1 MTR_4g072600 44.444 72 36 2 79 149 17 85 8.84e-15 66.2
MS.gene45855.t1 MTR_4g072670 47.059 68 32 2 82 148 21 85 1.19e-14 65.9
MS.gene45855.t1 MTR_4g072930 46.479 71 36 2 79 147 38 108 1.30e-14 66.6
MS.gene45855.t1 MTR_4g072860 46.970 66 31 2 82 146 26 88 1.59e-14 65.9
MS.gene45855.t1 MTR_4g072580 41.667 72 38 2 79 149 17 85 2.04e-14 65.5
MS.gene45855.t1 MTR_2g066830 42.105 76 41 1 82 157 58 130 2.13e-14 66.6
MS.gene45855.t1 MTR_2g043960 50.000 68 28 2 77 141 68 132 2.26e-14 67.0
MS.gene45855.t1 MTR_3g113310 38.298 94 46 3 57 148 22 105 2.37e-14 65.5
MS.gene45855.t1 MTR_4g072940 47.059 68 32 2 82 148 57 121 2.62e-14 65.9
MS.gene45855.t1 MTR_4g124750 51.667 60 26 1 82 141 69 125 2.99e-14 66.6
MS.gene45855.t1 MTR_3g117630 47.761 67 31 2 82 147 25 88 3.65e-14 64.7
MS.gene45855.t1 MTR_8g461210 39.744 78 44 1 80 157 33 107 4.12e-14 65.5
MS.gene45855.t1 MTR_4g072560 44.444 72 37 2 78 148 28 97 5.62e-14 64.3
MS.gene45855.t1 MTR_8g076040 46.269 67 33 1 82 148 41 104 5.91e-14 64.7
MS.gene45855.t1 MTR_3g084150 44.776 67 33 2 82 147 25 88 6.30e-14 64.3
MS.gene45855.t1 MTR_3g084180 44.776 67 33 2 82 147 25 88 7.63e-14 63.9
MS.gene45855.t1 MTR_4g072610 43.056 72 37 2 79 149 16 84 9.86e-14 63.5
MS.gene45855.t1 MTR_4g072590 50.820 61 26 2 82 141 20 77 9.93e-14 63.5
MS.gene45855.t1 MTR_5g021820 43.284 67 35 1 82 148 40 103 9.94e-14 63.9
MS.gene45855.t1 MTR_4g072480 46.970 66 31 2 82 146 25 87 1.06e-13 63.5
MS.gene45855.t1 MTR_1g061570 41.558 77 42 1 80 156 17 90 1.26e-13 64.3
MS.gene45855.t1 MTR_4g072630 45.070 71 35 2 79 148 18 85 1.34e-13 63.2
MS.gene45855.t1 MTR_1g009150 44.595 74 37 2 71 144 61 130 1.53e-13 65.5
MS.gene45855.t1 MTR_4g072720 45.588 68 33 2 82 148 21 85 1.79e-13 62.8
MS.gene45855.t1 MTR_1g024085 44.737 76 38 2 80 154 35 107 1.80e-13 64.3
MS.gene45855.t1 MTR_4g072730 46.970 66 31 2 82 146 25 87 1.91e-13 62.8
MS.gene45855.t1 MTR_4g072270 46.970 66 31 2 82 146 26 88 2.25e-13 62.8
MS.gene45855.t1 MTR_4g005320 47.619 63 30 1 82 144 70 129 2.55e-13 64.3
MS.gene45855.t1 MTR_4g072490 44.118 68 34 2 82 148 20 84 2.68e-13 62.4
MS.gene45855.t1 MTR_8g461410 43.284 67 34 2 82 147 25 88 2.74e-13 62.4
MS.gene45855.t1 MTR_4g072510 43.662 71 36 2 79 148 18 85 3.70e-13 62.0
MS.gene45855.t1 MTR_4g072660 46.970 66 31 2 82 146 20 82 4.13e-13 62.0
MS.gene45855.t1 MTR_5g008370 43.478 69 35 2 79 146 22 87 4.60e-13 63.2
MS.gene45855.t1 MTR_4g072620 45.455 66 32 2 82 146 25 87 4.62e-13 62.0
MS.gene45855.t1 MTR_4g072680 44.118 68 34 2 82 148 25 89 7.55e-13 61.2
MS.gene45855.t1 MTR_3g110042 45.588 68 33 2 82 148 25 89 8.84e-13 61.2
MS.gene45855.t1 MTR_1g059770 38.095 84 49 1 76 159 43 123 9.38e-13 62.0
MS.gene45855.t1 MTR_4g072460 44.118 68 34 2 82 148 25 89 9.74e-13 61.2
MS.gene45855.t1 MTR_4g072550 44.776 67 33 2 82 147 36 99 9.95e-13 61.2
MS.gene45855.t1 MTR_5g008360 41.176 68 36 2 82 148 16 80 1.11e-12 60.8
MS.gene45855.t1 MTR_4g072650 41.667 72 38 2 79 149 16 84 1.99e-12 60.1
MS.gene45855.t1 MTR_4g050700 46.970 66 31 2 82 146 26 88 2.29e-12 60.1
MS.gene45855.t1 MTR_2g012410 46.970 66 31 2 82 146 26 88 2.29e-12 60.1
MS.gene45855.t1 MTR_4g072450 42.647 68 35 2 82 148 20 84 2.48e-12 59.7
MS.gene45855.t1 MTR_8g096440 37.113 97 57 2 58 153 20 113 3.28e-12 60.8
MS.gene45855.t1 MTR_3g109160 42.105 76 40 2 78 153 65 136 6.34e-12 60.8
MS.gene45855.t1 MTR_5g008350 48.276 58 26 2 83 139 21 75 8.30e-12 58.2
MS.gene45855.t1 MTR_4g072470 41.176 68 36 2 82 148 20 84 9.26e-12 58.2
MS.gene45855.t1 MTR_4g057810 34.940 83 50 2 78 157 34 115 1.34e-11 58.9
MS.gene45855.t1 MTR_4g072770 40.000 70 38 2 82 150 15 81 1.37e-11 57.8
MS.gene45855.t1 MTR_7g405740 44.444 63 32 1 82 144 67 126 1.76e-11 59.3
MS.gene45855.t1 MTR_4g072280 53.061 49 20 1 79 127 23 68 2.20e-11 57.4
MS.gene45855.t1 MTR_4g072465 47.368 57 26 2 82 137 21 74 3.11e-11 57.0
MS.gene45855.t1 MTR_4g072750 49.057 53 24 1 79 131 24 73 3.84e-11 56.6
MS.gene45855.t1 MTR_4g072690 39.706 68 37 2 82 148 20 84 4.40e-11 56.6
MS.gene45855.t1 MTR_2g095430 40.625 64 37 1 82 144 45 108 4.85e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45855.t1 AT3G60690 52.597 154 67 4 3 152 7 158 7.05e-52 163
MS.gene45855.t1 AT2G45210 51.829 164 64 5 3 162 7 159 3.33e-50 159
MS.gene45855.t1 AT4G12410 45.513 156 71 5 3 156 7 150 1.68e-35 121
MS.gene45855.t1 AT4G22620 43.871 155 78 4 3 156 7 153 4.90e-33 115
MS.gene45855.t1 AT4G00880 58.904 73 29 1 76 148 22 93 2.54e-21 84.3
MS.gene45855.t1 AT2G46690 61.194 67 26 0 82 148 24 90 2.94e-21 84.0
MS.gene45855.t1 AT3G61900 59.701 67 27 0 82 148 30 96 1.10e-20 82.8
MS.gene45855.t1 AT5G53590 53.247 77 34 1 82 156 46 122 7.46e-20 80.9
MS.gene45855.t1 AT2G21210 50.000 72 34 2 82 152 28 98 7.83e-18 74.3
MS.gene45855.t1 AT2G21210 50.000 72 34 2 82 152 95 165 1.69e-17 75.5
MS.gene45855.t1 AT4G34800 50.685 73 35 1 70 141 9 81 1.80e-17 73.6
MS.gene45855.t1 AT4G38850 44.000 75 41 1 75 148 14 88 3.61e-17 72.4
MS.gene45855.t1 AT4G34790 59.016 61 23 2 82 141 39 98 9.75e-17 72.0
MS.gene45855.t1 AT4G34810 54.839 62 26 2 82 141 48 109 1.39e-16 71.6
MS.gene45855.t1 AT3G43120 52.941 68 26 2 77 141 71 135 4.15e-16 71.6
MS.gene45855.t1 AT5G18080 50.000 72 33 2 78 148 20 89 4.52e-16 69.7
MS.gene45855.t1 AT3G03820 53.731 67 30 1 83 148 28 94 4.94e-16 69.7
MS.gene45855.t1 AT3G20220 44.578 83 41 3 66 147 32 110 8.05e-16 69.7
MS.gene45855.t1 AT3G03850 46.250 80 40 3 70 148 14 91 1.19e-15 68.6
MS.gene45855.t1 AT4G34780 48.529 68 34 1 82 148 28 95 1.25e-15 68.9
MS.gene45855.t1 AT4G34770 50.000 72 33 3 82 152 35 104 1.49e-15 68.9
MS.gene45855.t1 AT5G10990 45.349 86 41 2 80 162 39 121 1.69e-15 69.7
MS.gene45855.t1 AT5G18010 47.222 72 35 2 78 148 20 89 1.88e-15 68.2
MS.gene45855.t1 AT2G21220 44.000 75 37 2 76 148 32 103 2.19e-15 68.2
MS.gene45855.t1 AT4G38825 49.254 67 31 2 83 148 24 88 3.27e-15 67.4
MS.gene45855.t1 AT4G38840 49.254 67 31 2 82 147 33 97 3.29e-15 67.8
MS.gene45855.t1 AT5G20810 51.471 68 27 2 77 141 71 135 3.32e-15 69.3
MS.gene45855.t1 AT5G18020 47.887 71 34 2 78 147 20 88 4.90e-15 67.0
MS.gene45855.t1 AT5G18050 48.571 70 33 2 80 148 22 89 5.54e-15 67.0
MS.gene45855.t1 AT5G20810 51.471 68 27 2 77 141 71 135 6.82e-15 69.3
MS.gene45855.t1 AT1G56150 42.500 80 43 1 76 155 32 108 2.60e-14 65.9
MS.gene45855.t1 AT5G18060 49.254 67 31 2 83 148 25 89 2.73e-14 65.1
MS.gene45855.t1 AT5G18030 45.833 72 36 2 78 148 18 87 3.08e-14 65.1
MS.gene45855.t1 AT3G03840 50.746 67 31 2 83 148 28 93 3.59e-14 65.1
MS.gene45855.t1 AT4G34760 44.776 67 34 1 82 148 43 106 4.43e-14 65.1
MS.gene45855.t1 AT1G75580 38.889 108 55 5 46 148 6 107 1.33e-13 63.9
MS.gene45855.t1 AT1G75590 49.206 63 29 1 80 142 40 99 1.93e-13 64.7
MS.gene45855.t1 AT2G21200 46.269 67 33 2 83 148 22 86 2.91e-13 62.4
MS.gene45855.t1 AT4G38860 44.737 76 36 3 76 148 32 104 3.12e-13 62.8
MS.gene45855.t1 AT4G34750 45.946 74 33 2 71 144 30 96 3.22e-13 63.9
MS.gene45855.t1 AT4G34750 45.946 74 33 2 71 144 30 96 3.22e-13 63.9
MS.gene45855.t1 AT4G13790 47.761 67 33 2 83 148 26 91 5.74e-13 61.6
MS.gene45855.t1 AT1G19830 43.284 67 35 1 82 148 48 111 1.54e-12 61.2
MS.gene45855.t1 AT3G12830 41.667 72 39 1 80 151 48 116 1.63e-12 61.6
MS.gene45855.t1 AT3G03830 45.714 70 37 1 80 148 22 91 1.75e-12 60.5
MS.gene45855.t1 AT1G19840 47.619 63 30 1 80 142 39 98 2.05e-12 61.6
MS.gene45855.t1 AT4G36110 46.774 62 30 1 80 141 38 96 3.12e-12 60.1
MS.gene45855.t1 AT5G66260 37.838 74 43 1 75 148 27 97 3.81e-12 59.7
MS.gene45855.t1 AT1G16510 42.857 70 37 1 82 151 54 120 5.43e-12 60.5
MS.gene45855.t1 AT2G24400 47.619 63 30 1 82 144 64 123 2.26e-11 59.7
MS.gene45855.t1 AT2G18010 49.091 55 25 1 82 136 48 99 2.88e-11 57.8
MS.gene45855.t1 AT4G31320 43.077 65 34 1 80 144 78 139 3.64e-11 59.3
MS.gene45855.t1 AT2G16580 33.663 101 56 2 48 148 18 107 4.71e-11 57.0
MS.gene45855.t1 AT2G37030 42.857 63 33 1 82 144 49 108 7.58e-11 57.0

Find 65 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTCAATCATCCTTTGTT+TGG 0.205259 4.4:+75847392 MS.gene45855:CDS
CGGTGAGTTTCGGCGGGTTT+TGG 0.248448 4.4:+75847358 MS.gene45855:CDS
ACTTCAATCATCCTTTGTTT+GGG 0.255039 4.4:+75847393 MS.gene45855:CDS
AAAGCAGGGTCGGGTAAATT+TGG 0.257203 4.4:+75847248 MS.gene45855:CDS
AAGTGATTCGGGTCAGTAAA+TGG 0.277611 4.4:+75847096 MS.gene45855:CDS
AATGGATATTCCGGCGATTT+CGG 0.281583 4.4:+75847114 MS.gene45855:CDS
AAAGAAGCTGAGAAAGAGTA+TGG 0.306967 4.4:+75847425 MS.gene45855:CDS
GGTGAACCGCTTTGCTCAAC+CGG 0.334212 4.4:-75847293 None:intergenic
TATCCGACCCAGCTGCTATT+CGG 0.335564 4.4:-75847511 None:intergenic
ATCCGACCCAGCTGCTATTC+GGG 0.369954 4.4:-75847510 None:intergenic
CGAGACGACGGTATCTTGTT+CGG 0.398391 4.4:-75847139 None:intergenic
TCGGGTTACCGAGTTTGAAC+GGG 0.414517 4.4:+75847481 MS.gene45855:CDS
GTCGGGTTACCGAGTTTGAA+CGG 0.425737 4.4:+75847480 MS.gene45855:CDS
GCTAGGGAAGAAAGTGATTC+GGG 0.437214 4.4:+75847085 MS.gene45855:CDS
AGCTAGGGAAGAAAGTGATT+CGG 0.439484 4.4:+75847084 MS.gene45855:CDS
CTTCCCTAGCTTGAATTGCA+TGG 0.441083 4.4:-75847072 None:intergenic
GTCGTCAAATGATTCTTCTC+CGG 0.445127 4.4:-75847170 None:intergenic
GAGACGACGGTATCTTGTTC+GGG 0.448535 4.4:-75847138 None:intergenic
GGGTCAGTAAATGGATATTC+CGG 0.450545 4.4:+75847105 MS.gene45855:CDS
TGGAATCACTATTCCTTGTC+GGG 0.453830 4.4:+75847463 MS.gene45855:CDS
GTGGAATCACTATTCCTTGT+CGG 0.471607 4.4:+75847462 MS.gene45855:CDS
GGGCATTTAGCGGTGTATGT+GGG 0.472930 4.4:+75847326 MS.gene45855:CDS
AAGTCTCTATGTTCAAAAGC+AGG 0.487934 4.4:+75847233 MS.gene45855:CDS
AATTTGGTTATTTGCCGATA+GGG 0.495078 4.4:+75847264 MS.gene45855:CDS
CCGTCGTCTCGGCGCCTCAC+CGG 0.497600 4.4:+75847151 MS.gene45855:CDS
GGGGCATTTAGCGGTGTATG+TGG 0.497853 4.4:+75847325 MS.gene45855:CDS
GCTAAATGCCCCTTCGGTAC+CGG 0.500069 4.4:-75847314 None:intergenic
TACACCGCTAAATGCCCCTT+CGG 0.500996 4.4:-75847320 None:intergenic
GTTAAGACCCGAATAGCAGC+TGG 0.501036 4.4:+75847503 MS.gene45855:CDS
TGACGACAAAGCTTCTATCA+TGG 0.502757 4.4:+75847186 MS.gene45855:CDS
GACGACAAAGCTTCTATCAT+GGG 0.503605 4.4:+75847187 MS.gene45855:CDS
AAATTTGGTTATTTGCCGAT+AGG 0.508006 4.4:+75847263 MS.gene45855:CDS
ATAACCATGCAATTCAAGCT+AGG 0.509622 4.4:+75847068 None:intergenic
GGTCTTAACCCGTTCAAACT+CGG 0.514111 4.4:-75847489 None:intergenic
CTATGTTCAAAAGCAGGGTC+GGG 0.516192 4.4:+75847239 MS.gene45855:CDS
CTTCAATCATCCTTTGTTTG+GGG 0.517007 4.4:+75847394 MS.gene45855:CDS
TGGGTCGGATACACGAAGAT+TGG 0.536901 4.4:+75847523 MS.gene45855:CDS
AAGCGGTTCACCGGTACCGA+AGG 0.543995 4.4:+75847304 MS.gene45855:CDS
GGCATTTAGCGGTGTATGTG+GGG 0.555901 4.4:+75847327 MS.gene45855:CDS
AAAAGACGGTGAGTTTCGGC+GGG 0.560575 4.4:+75847352 MS.gene45855:CDS
TCTATGTTCAAAAGCAGGGT+CGG 0.560582 4.4:+75847238 MS.gene45855:CDS
TTGTTCGGGTCCGAAATCGC+CGG 0.561790 4.4:-75847124 None:intergenic
TAACCATGCAATTCAAGCTA+GGG 0.566394 4.4:+75847069 None:intergenic
GGTTGAGCAAAGCGGTTCAC+CGG 0.573801 4.4:+75847295 MS.gene45855:CDS
TTCAACAACTCCCCAAACAA+AGG 0.574136 4.4:-75847404 None:intergenic
GACCCGAATAGCAGCTGGGT+CGG 0.582499 4.4:+75847508 MS.gene45855:CDS
AGCGGTTCACCGGTACCGAA+GGG 0.590068 4.4:+75847305 MS.gene45855:CDS
GGTACCGAAGGGGCATTTAG+CGG 0.599134 4.4:+75847316 MS.gene45855:CDS
CCGGTGAGGCGCCGAGACGA+CGG 0.603271 4.4:-75847151 None:intergenic
AAGAAGCTGAGAAAGAGTAT+GGG 0.603282 4.4:+75847426 MS.gene45855:CDS
ACCGCTTTGCTCAACCGGGT+CGG 0.604772 4.4:-75847288 None:intergenic
TCAACCGGGTCGGACCCTAT+CGG 0.605566 4.4:-75847278 None:intergenic
GTGAACCGCTTTGCTCAACC+GGG 0.606769 4.4:-75847292 None:intergenic
ACGACAAAGCTTCTATCATG+GGG 0.613722 4.4:+75847188 MS.gene45855:CDS
CGAACAAGATACCGTCGTCT+CGG 0.613951 4.4:+75847140 MS.gene45855:CDS
AGTCTCTATGTTCAAAAGCA+GGG 0.613958 4.4:+75847234 MS.gene45855:CDS
TCAAACTCGGTAACCCGACA+AGG 0.618391 4.4:-75847476 None:intergenic
CAAATGATTCTTCTCCGGTG+AGG 0.645717 4.4:-75847165 None:intergenic
TTTGCCGATAGGGTCCGACC+CGG 0.646098 4.4:+75847274 MS.gene45855:CDS
GGATGATTGAAGTAAACCAC+CGG 0.649322 4.4:-75847383 None:intergenic
GCGGTTCACCGGTACCGAAG+GGG 0.656345 4.4:+75847306 MS.gene45855:CDS
TTAAGACCCGAATAGCAGCT+GGG 0.660451 4.4:+75847504 MS.gene45855:CDS
ATACACGAAGATTGGCGAAG+CGG 0.667833 4.4:+75847531 MS.gene45855:CDS
TCCGACCCGGTTGAGCAAAG+CGG 0.712572 4.4:+75847287 MS.gene45855:CDS
CGACAAAGCTTCTATCATGG+GGG 0.730867 4.4:+75847189 MS.gene45855:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAATTTGGTTATTTGCCGAT+AGG + chr4.4:75847263-75847282 MS.gene45855:CDS 30.0%
AATTTGGTTATTTGCCGATA+GGG + chr4.4:75847264-75847283 MS.gene45855:CDS 30.0%
TACTTCAATCATCCTTTGTT+TGG + chr4.4:75847392-75847411 MS.gene45855:CDS 30.0%
! ACTTCAATCATCCTTTGTTT+GGG + chr4.4:75847393-75847412 MS.gene45855:CDS 30.0%
AAAGAAGCTGAGAAAGAGTA+TGG + chr4.4:75847425-75847444 MS.gene45855:CDS 35.0%
AAGTCTCTATGTTCAAAAGC+AGG + chr4.4:75847233-75847252 MS.gene45855:CDS 35.0%
AGTCTCTATGTTCAAAAGCA+GGG + chr4.4:75847234-75847253 MS.gene45855:CDS 35.0%
! AAGAAGCTGAGAAAGAGTAT+GGG + chr4.4:75847426-75847445 MS.gene45855:CDS 35.0%
! CTTCAATCATCCTTTGTTTG+GGG + chr4.4:75847394-75847413 MS.gene45855:CDS 35.0%
AATGGATATTCCGGCGATTT+CGG + chr4.4:75847114-75847133 MS.gene45855:CDS 40.0%
ACGACAAAGCTTCTATCATG+GGG + chr4.4:75847188-75847207 MS.gene45855:CDS 40.0%
AGCTAGGGAAGAAAGTGATT+CGG + chr4.4:75847084-75847103 MS.gene45855:CDS 40.0%
GACGACAAAGCTTCTATCAT+GGG + chr4.4:75847187-75847206 MS.gene45855:CDS 40.0%
GGATGATTGAAGTAAACCAC+CGG - chr4.4:75847386-75847405 None:intergenic 40.0%
GGGTCAGTAAATGGATATTC+CGG + chr4.4:75847105-75847124 MS.gene45855:CDS 40.0%
GTCGTCAAATGATTCTTCTC+CGG - chr4.4:75847173-75847192 None:intergenic 40.0%
GTGGAATCACTATTCCTTGT+CGG + chr4.4:75847462-75847481 MS.gene45855:CDS 40.0%
TCTATGTTCAAAAGCAGGGT+CGG + chr4.4:75847238-75847257 MS.gene45855:CDS 40.0%
TGACGACAAAGCTTCTATCA+TGG + chr4.4:75847186-75847205 MS.gene45855:CDS 40.0%
TGGAATCACTATTCCTTGTC+GGG + chr4.4:75847463-75847482 MS.gene45855:CDS 40.0%
TTCAACAACTCCCCAAACAA+AGG - chr4.4:75847407-75847426 None:intergenic 40.0%
! AAGTGATTCGGGTCAGTAAA+TGG + chr4.4:75847096-75847115 MS.gene45855:CDS 40.0%
!! GAGTATGGGTTTTGTCATCA+AGG + chr4.4:75847440-75847459 MS.gene45855:CDS 40.0%
!! TATGGGTTTTGTCATCAAGG+TGG + chr4.4:75847443-75847462 MS.gene45855:CDS 40.0%
AAAAAGACGGTGAGTTTCGG+CGG + chr4.4:75847351-75847370 MS.gene45855:CDS 45.0%
AAAGCAGGGTCGGGTAAATT+TGG + chr4.4:75847248-75847267 MS.gene45855:CDS 45.0%
ATACACGAAGATTGGCGAAG+CGG + chr4.4:75847531-75847550 MS.gene45855:CDS 45.0%
CAAATGATTCTTCTCCGGTG+AGG - chr4.4:75847168-75847187 None:intergenic 45.0%
CGACAAAGCTTCTATCATGG+GGG + chr4.4:75847189-75847208 MS.gene45855:CDS 45.0%
CTATGTTCAAAAGCAGGGTC+GGG + chr4.4:75847239-75847258 MS.gene45855:CDS 45.0%
GCTAGGGAAGAAAGTGATTC+GGG + chr4.4:75847085-75847104 MS.gene45855:CDS 45.0%
GGCAAAAAGACGGTGAGTTT+CGG + chr4.4:75847348-75847367 MS.gene45855:CDS 45.0%
GGTCTTAACCCGTTCAAACT+CGG - chr4.4:75847492-75847511 None:intergenic 45.0%
GTGTATGTGGGGCAAAAAGA+CGG + chr4.4:75847338-75847357 MS.gene45855:CDS 45.0%
TTAAGACCCGAATAGCAGCT+GGG + chr4.4:75847504-75847523 MS.gene45855:CDS 45.0%
AAAAGACGGTGAGTTTCGGC+GGG + chr4.4:75847352-75847371 MS.gene45855:CDS 50.0%
CGAACAAGATACCGTCGTCT+CGG + chr4.4:75847140-75847159 MS.gene45855:CDS 50.0%
CGAGACGACGGTATCTTGTT+CGG - chr4.4:75847142-75847161 None:intergenic 50.0%
GAGACGACGGTATCTTGTTC+GGG - chr4.4:75847141-75847160 None:intergenic 50.0%
GGCATTTAGCGGTGTATGTG+GGG + chr4.4:75847327-75847346 MS.gene45855:CDS 50.0%
GGGCATTTAGCGGTGTATGT+GGG + chr4.4:75847326-75847345 MS.gene45855:CDS 50.0%
GTCGGGTTACCGAGTTTGAA+CGG + chr4.4:75847480-75847499 MS.gene45855:CDS 50.0%
GTTAAGACCCGAATAGCAGC+TGG + chr4.4:75847503-75847522 MS.gene45855:CDS 50.0%
TACACCGCTAAATGCCCCTT+CGG - chr4.4:75847323-75847342 None:intergenic 50.0%
TATCCGACCCAGCTGCTATT+CGG - chr4.4:75847514-75847533 None:intergenic 50.0%
TCAAACTCGGTAACCCGACA+AGG - chr4.4:75847479-75847498 None:intergenic 50.0%
TCGGGTTACCGAGTTTGAAC+GGG + chr4.4:75847481-75847500 MS.gene45855:CDS 50.0%
TGGGTCGGATACACGAAGAT+TGG + chr4.4:75847523-75847542 MS.gene45855:CDS 50.0%
ATCCGACCCAGCTGCTATTC+GGG - chr4.4:75847513-75847532 None:intergenic 55.0%
GCTAAATGCCCCTTCGGTAC+CGG - chr4.4:75847317-75847336 None:intergenic 55.0%
GGTGAACCGCTTTGCTCAAC+CGG - chr4.4:75847296-75847315 None:intergenic 55.0%
GGTTGAGCAAAGCGGTTCAC+CGG + chr4.4:75847295-75847314 MS.gene45855:CDS 55.0%
GTGAACCGCTTTGCTCAACC+GGG - chr4.4:75847295-75847314 None:intergenic 55.0%
TTGTTCGGGTCCGAAATCGC+CGG - chr4.4:75847127-75847146 None:intergenic 55.0%
! GGGGCATTTAGCGGTGTATG+TGG + chr4.4:75847325-75847344 MS.gene45855:CDS 55.0%
!! GGTACCGAAGGGGCATTTAG+CGG + chr4.4:75847316-75847335 MS.gene45855:CDS 55.0%
ACCGCTTTGCTCAACCGGGT+CGG - chr4.4:75847291-75847310 None:intergenic 60.0%
GACCCGAATAGCAGCTGGGT+CGG + chr4.4:75847508-75847527 MS.gene45855:CDS 60.0%
TCAACCGGGTCGGACCCTAT+CGG - chr4.4:75847281-75847300 None:intergenic 60.0%
TCCGACCCGGTTGAGCAAAG+CGG + chr4.4:75847287-75847306 MS.gene45855:CDS 60.0%
TTTGCCGATAGGGTCCGACC+CGG + chr4.4:75847274-75847293 MS.gene45855:CDS 60.0%
! AAGCGGTTCACCGGTACCGA+AGG + chr4.4:75847304-75847323 MS.gene45855:CDS 60.0%
! AGCGGTTCACCGGTACCGAA+GGG + chr4.4:75847305-75847324 MS.gene45855:CDS 60.0%
!! CGGTGAGTTTCGGCGGGTTT+TGG + chr4.4:75847358-75847377 MS.gene45855:CDS 60.0%
!! GTTTCGGCGGGTTTTGGTGC+CGG + chr4.4:75847364-75847383 MS.gene45855:CDS 60.0%
! GCGGTTCACCGGTACCGAAG+GGG + chr4.4:75847306-75847325 MS.gene45855:CDS 65.0%
!!! TCGGCGGGTTTTGGTGCCGG+TGG + chr4.4:75847367-75847386 MS.gene45855:CDS 70.0%
CCGTCGTCTCGGCGCCTCAC+CGG + chr4.4:75847151-75847170 MS.gene45855:CDS 75.0%
!! CCGGTGAGGCGCCGAGACGA+CGG - chr4.4:75847154-75847173 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 75847074 75847571 75847074 ID=MS.gene45855
chr4.4 mRNA 75847074 75847571 75847074 ID=MS.gene45855.t1;Parent=MS.gene45855
chr4.4 exon 75847074 75847571 75847074 ID=MS.gene45855.t1.exon1;Parent=MS.gene45855.t1
chr4.4 CDS 75847074 75847571 75847074 ID=cds.MS.gene45855.t1;Parent=MS.gene45855.t1
Gene Sequence

>MS.gene45855

ATGCAATTCAAGCTAGGGAAGAAAGTGATTCGGGTCAGTAAATGGATATTCCGGCGATTTCGGACCCGAACAAGATACCGTCGTCTCGGCGCCTCACCGGAGAAGAATCATTTGACGACAAAGCTTCTATCATGGGGGAATAAGTTAACTACAAAAGCAAAGTCTCTATGTTCAAAAGCAGGGTCGGGTAAATTTGGTTATTTGCCGATAGGGTCCGACCCGGTTGAGCAAAGCGGTTCACCGGTACCGAAGGGGCATTTAGCGGTGTATGTGGGGCAAAAAGACGGTGAGTTTCGGCGGGTTTTGGTGCCGGTGGTTTACTTCAATCATCCTTTGTTTGGGGAGTTGTTGAAAGAAGCTGAGAAAGAGTATGGGTTTTGTCATCAAGGTGGAATCACTATTCCTTGTCGGGTTACCGAGTTTGAACGGGTTAAGACCCGAATAGCAGCTGGGTCGGATACACGAAGATTGGCGAAGCGGAGACATTTTGATTGCTGA
Protein sequence

>MS.gene45855.t1

MQFKLGKKVIRVSKWIFRRFRTRTRYRRLGASPEKNHLTTKLLSWGNKLTTKAKSLCSKAGSGKFGYLPIGSDPVEQSGSPVPKGHLAVYVGQKDGEFRRVLVPVVYFNHPLFGELLKEAEKEYGFCHQGGITIPCRVTEFERVKTRIAAGSDTRRLAKRRHFDC