AlfalfaGEDB | MS.gene059224

M. sativa cultivar XinJiangDaYe / MS.gene059224

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059224.t1	XP_013455080.1	93.3	164	8	1	1	164	1	161	3.30E-82	314.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059224.t1	O22150	51.8	164	64	5	3	162	7	159	1.9e-38	160.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059224.t1	I3T7X1	93.3	164	8	1	1	164	1	161	2.4e-82	314.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene059224	MS.gene069412	0.812004	5.27E-51	-1.69E-46
MS.gene059224	MS.gene069415	0.813144	2.97E-51	-1.69E-46
MS.gene059224	MS.gene30397	0.806186	9.29E-50	-1.69E-46
MS.gene059224	MS.gene31855	0.800237	1.58E-48	-1.69E-46
MS.gene059224	MS.gene32238	0.804561	2.04E-49	-1.69E-46
MS.gene059224	MS.gene33644	0.819276	1.26E-52	-1.69E-46
MS.gene059224	MS.gene33651	0.811655	6.28E-51	-1.69E-46
MS.gene059224	MS.gene36289	0.814011	1.91E-51	-1.69E-46
MS.gene059224	MS.gene38998	0.804706	1.90E-49	-1.69E-46
MS.gene059224	MS.gene40381	-0.802627	5.13E-49	-1.69E-46
MS.gene059224	MS.gene44621	0.82026	7.54E-53	-1.69E-46
MS.gene059224	MS.gene45855	0.930564	1.36E-93	-1.69E-46
MS.gene059224	MS.gene48066	-0.834017	3.82E-56	-1.69E-46
MS.gene059224	MS.gene51785	0.80305	4.19E-49	-1.69E-46
MS.gene059224	MS.gene53537	0.802145	6.45E-49	-1.69E-46
MS.gene059224	MS.gene78901	0.811608	6.43E-51	-1.69E-46
MS.gene059224	MS.gene83473	0.817895	2.60E-52	-1.69E-46
MS.gene059224	MS.gene87076	0.80997	1.45E-50	-1.69E-46
MS.gene059224	MS.gene87732	0.810287	1.24E-50	-1.69E-46
MS.gene059224	MS.gene89104	0.80338	3.58E-49	-1.69E-46
MS.gene059224	MS.gene94111	0.818209	2.21E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059224.t1	MTR_4g025830	93.293	164	8	1	1	164	1	161	1.43e-110	311
MS.gene059224.t1	MTR_0388s0020	51.333	150	62	3	3	152	7	145	7.22e-46	147
MS.gene059224.t1	MTR_7g118010	53.571	84	35	2	82	165	23	102	4.69e-23	88.2
MS.gene059224.t1	MTR_3g084250	55.882	68	26	2	82	148	25	89	4.60e-21	82.4
MS.gene059224.t1	MTR_3g092220	50.000	84	37	2	82	165	33	111	2.63e-20	81.3
MS.gene059224.t1	MTR_8g026730	51.190	84	36	2	82	165	27	105	8.18e-20	79.7
MS.gene059224.t1	MTR_3g084210	57.377	61	22	2	82	141	25	82	9.81e-19	76.6
MS.gene059224.t1	MTR_3g084230	57.377	61	22	2	82	141	25	82	9.92e-19	76.6
MS.gene059224.t1	MTR_4g072220	50.000	72	32	2	79	149	23	91	1.13e-18	76.3
MS.gene059224.t1	MTR_4g072240	50.704	71	31	2	79	148	23	90	1.62e-18	75.9
MS.gene059224.t1	MTR_3g084160	57.377	61	22	2	82	141	25	82	1.66e-18	75.9
MS.gene059224.t1	MTR_4g072300	50.704	71	31	2	79	148	23	90	3.16e-18	75.1
MS.gene059224.t1	MTR_8g461420	55.738	61	23	2	82	141	25	82	6.96e-18	74.3
MS.gene059224.t1	MTR_3g084190	55.738	61	23	2	82	141	25	82	1.26e-17	73.6
MS.gene059224.t1	MTR_3g110045	55.738	61	23	2	82	141	84	141	3.21e-17	74.3
MS.gene059224.t1	MTR_4g072570	48.529	68	31	2	82	148	25	89	3.97e-17	72.4
MS.gene059224.t1	MTR_4g072980	45.333	75	38	2	77	150	22	94	1.17e-16	71.2
MS.gene059224.t1	MTR_4g072850	48.529	68	31	2	82	148	27	91	1.20e-16	71.2
MS.gene059224.t1	MTR_4g072500	50.000	66	29	2	82	146	25	87	1.99e-16	70.5
MS.gene059224.t1	MTR_4g072330	47.059	68	32	2	82	148	23	87	2.50e-16	70.5
MS.gene059224.t1	MTR_3g117640	48.571	70	33	2	82	150	34	101	2.76e-16	70.5
MS.gene059224.t1	MTR_4g072530	51.515	66	28	2	82	146	25	87	3.23e-16	70.1
MS.gene059224.t1	MTR_4g072910	48.529	68	31	2	82	148	35	99	3.68e-16	70.1
MS.gene059224.t1	MTR_4g072230	46.269	67	30	2	82	147	26	87	4.15e-16	69.7
MS.gene059224.t1	MTR_4g072890	47.887	71	33	2	79	148	23	90	5.09e-16	69.7
MS.gene059224.t1	MTR_4g072250	45.588	68	32	2	82	148	26	89	6.54e-16	69.3
MS.gene059224.t1	MTR_4g072830	41.772	79	42	2	71	148	12	87	6.55e-16	69.3
MS.gene059224.t1	MTR_4g072640	48.485	66	30	2	82	146	25	87	8.37e-16	68.9
MS.gene059224.t1	MTR_4g072260	45.833	72	35	2	79	149	23	91	1.26e-15	68.6
MS.gene059224.t1	MTR_4g072820	47.059	68	32	2	82	148	20	84	1.73e-15	68.2
MS.gene059224.t1	MTR_4g072160	43.678	87	42	2	82	164	37	120	1.99e-15	69.3
MS.gene059224.t1	MTR_4g072310	45.588	68	33	2	82	148	26	90	2.09e-15	68.2
MS.gene059224.t1	MTR_4g072370	40.506	79	43	2	71	148	12	87	2.35e-15	67.8
MS.gene059224.t1	MTR_4g072870	46.479	71	34	2	79	148	23	90	2.41e-15	67.8
MS.gene059224.t1	MTR_3g117610	50.000	60	27	1	82	141	45	101	2.68e-15	68.2
MS.gene059224.t1	MTR_4g072880	47.059	68	32	2	82	148	26	90	2.76e-15	67.8
MS.gene059224.t1	MTR_4g072190	44.776	67	34	1	82	148	36	99	4.03e-15	67.4
MS.gene059224.t1	MTR_4g072600	44.444	72	36	2	79	149	17	85	8.56e-15	66.2
MS.gene059224.t1	MTR_4g072670	47.059	68	32	2	82	148	21	85	1.16e-14	65.9
MS.gene059224.t1	MTR_4g072930	46.479	71	36	2	79	147	38	108	1.33e-14	66.6
MS.gene059224.t1	MTR_4g072860	46.970	66	31	2	82	146	26	88	1.56e-14	65.9
MS.gene059224.t1	MTR_4g072580	41.667	72	38	2	79	149	17	85	2.01e-14	65.5
MS.gene059224.t1	MTR_2g066830	42.105	76	41	1	82	157	58	130	2.06e-14	66.6
MS.gene059224.t1	MTR_3g113310	38.298	94	46	3	57	148	22	105	2.25e-14	65.9
MS.gene059224.t1	MTR_2g043960	50.000	68	28	2	77	141	68	132	2.26e-14	67.0
MS.gene059224.t1	MTR_4g072940	47.059	68	32	2	82	148	57	121	2.65e-14	65.9
MS.gene059224.t1	MTR_4g124750	51.667	60	26	1	82	141	69	125	2.99e-14	66.6
MS.gene059224.t1	MTR_3g117630	47.761	67	31	2	82	147	25	88	3.58e-14	64.7
MS.gene059224.t1	MTR_8g461210	39.744	78	44	1	80	157	33	107	4.39e-14	65.1
MS.gene059224.t1	MTR_4g072560	44.444	72	37	2	78	148	28	97	5.33e-14	64.7
MS.gene059224.t1	MTR_3g084150	44.776	67	33	2	82	147	25	88	6.17e-14	64.3
MS.gene059224.t1	MTR_8g076040	46.269	67	33	1	82	148	41	104	6.30e-14	64.7
MS.gene059224.t1	MTR_3g084180	44.776	67	33	2	82	147	25	88	7.63e-14	63.9
MS.gene059224.t1	MTR_4g072590	50.820	61	26	2	82	141	20	77	9.41e-14	63.5
MS.gene059224.t1	MTR_4g072610	43.056	72	37	2	79	149	16	84	9.75e-14	63.5
MS.gene059224.t1	MTR_5g021820	43.284	67	35	1	82	148	40	103	1.06e-13	63.9
MS.gene059224.t1	MTR_4g072480	46.970	66	31	2	82	146	25	87	1.09e-13	63.5
MS.gene059224.t1	MTR_1g061570	41.558	77	42	1	80	156	17	90	1.39e-13	64.3
MS.gene059224.t1	MTR_4g072630	51.667	60	25	2	79	137	18	74	1.40e-13	63.2
MS.gene059224.t1	MTR_1g009150	44.595	74	37	2	71	144	61	130	1.55e-13	65.5
MS.gene059224.t1	MTR_1g024085	44.737	76	38	2	80	154	35	107	1.71e-13	64.3
MS.gene059224.t1	MTR_4g072720	45.588	68	33	2	82	148	21	85	1.77e-13	62.8
MS.gene059224.t1	MTR_4g072730	46.970	66	31	2	82	146	25	87	1.95e-13	62.8
MS.gene059224.t1	MTR_4g072270	46.970	66	31	2	82	146	26	88	2.22e-13	62.8
MS.gene059224.t1	MTR_4g005320	47.619	63	30	1	82	144	70	129	2.47e-13	64.3
MS.gene059224.t1	MTR_4g072490	44.118	68	34	2	82	148	20	84	2.68e-13	62.4
MS.gene059224.t1	MTR_8g461410	43.284	67	34	2	82	147	25	88	2.74e-13	62.4
MS.gene059224.t1	MTR_4g072510	43.662	71	36	2	79	148	18	85	3.74e-13	62.0
MS.gene059224.t1	MTR_4g072660	46.970	66	31	2	82	146	20	82	4.13e-13	62.0
MS.gene059224.t1	MTR_5g008370	43.478	69	35	2	79	146	22	87	4.31e-13	63.2
MS.gene059224.t1	MTR_4g072620	45.455	66	32	2	82	146	25	87	4.48e-13	62.0
MS.gene059224.t1	MTR_4g072680	44.118	68	34	2	82	148	25	89	7.15e-13	61.2
MS.gene059224.t1	MTR_3g110042	45.588	68	33	2	82	148	25	89	8.56e-13	61.2
MS.gene059224.t1	MTR_4g072550	44.776	67	33	2	82	147	36	99	9.85e-13	61.2
MS.gene059224.t1	MTR_4g072460	44.118	68	34	2	82	148	25	89	1.01e-12	60.8
MS.gene059224.t1	MTR_5g008360	41.176	68	36	2	82	148	16	80	1.05e-12	60.8
MS.gene059224.t1	MTR_4g072650	41.667	72	38	2	79	149	16	84	1.91e-12	60.1
MS.gene059224.t1	MTR_1g059770	36.905	84	50	1	76	159	43	123	1.94e-12	61.2
MS.gene059224.t1	MTR_4g050700	46.970	66	31	2	82	146	26	88	2.31e-12	60.1
MS.gene059224.t1	MTR_2g012410	46.970	66	31	2	82	146	26	88	2.31e-12	60.1
MS.gene059224.t1	MTR_4g072450	42.647	68	35	2	82	148	20	84	2.48e-12	59.7
MS.gene059224.t1	MTR_8g096440	37.113	97	57	2	58	153	20	113	3.28e-12	60.8
MS.gene059224.t1	MTR_3g109160	42.105	76	40	2	78	153	65	136	6.28e-12	60.8
MS.gene059224.t1	MTR_5g008350	48.276	58	26	2	83	139	21	75	8.38e-12	58.2
MS.gene059224.t1	MTR_4g072470	41.176	68	36	2	82	148	20	84	8.69e-12	58.5
MS.gene059224.t1	MTR_4g072770	40.000	70	38	2	82	150	15	81	1.36e-11	57.8
MS.gene059224.t1	MTR_4g057810	34.940	83	50	2	78	157	34	115	1.39e-11	58.9
MS.gene059224.t1	MTR_7g405740	44.444	63	32	1	82	144	67	126	1.67e-11	59.7
MS.gene059224.t1	MTR_4g072280	53.061	49	20	1	79	127	23	68	2.22e-11	57.0
MS.gene059224.t1	MTR_4g072465	47.368	57	26	2	82	137	21	74	3.05e-11	57.0
MS.gene059224.t1	MTR_4g072750	49.057	53	24	1	79	131	24	73	3.76e-11	56.6
MS.gene059224.t1	MTR_4g072690	39.706	68	37	2	82	148	20	84	4.22e-11	56.6
MS.gene059224.t1	MTR_2g095430	40.625	64	37	1	82	144	45	108	4.80e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059224.t1	AT3G60690	52.597	154	67	4	3	152	7	158	6.67e-52	163
MS.gene059224.t1	AT2G45210	51.829	164	64	5	3	162	7	159	3.97e-50	159
MS.gene059224.t1	AT4G12410	45.513	156	71	5	3	156	7	150	1.87e-35	121
MS.gene059224.t1	AT4G22620	43.871	155	78	4	3	156	7	153	5.06e-33	115
MS.gene059224.t1	AT4G00880	58.904	73	29	1	76	148	22	93	2.46e-21	84.3
MS.gene059224.t1	AT2G46690	61.194	67	26	0	82	148	24	90	2.78e-21	84.0
MS.gene059224.t1	AT3G61900	59.701	67	27	0	82	148	30	96	1.09e-20	82.8
MS.gene059224.t1	AT5G53590	53.247	77	34	1	82	156	46	122	7.30e-20	80.9
MS.gene059224.t1	AT2G21210	50.000	72	34	2	82	152	28	98	7.83e-18	74.3
MS.gene059224.t1	AT2G21210	50.000	72	34	2	82	152	95	165	1.73e-17	75.5
MS.gene059224.t1	AT4G34800	50.685	73	35	1	70	141	9	81	2.02e-17	73.2
MS.gene059224.t1	AT4G38850	44.000	75	41	1	75	148	14	88	3.57e-17	72.4
MS.gene059224.t1	AT4G34790	59.016	61	23	2	82	141	39	98	1.09e-16	71.6
MS.gene059224.t1	AT4G34810	54.839	62	26	2	82	141	48	109	1.38e-16	72.0
MS.gene059224.t1	AT3G43120	52.941	68	26	2	77	141	71	135	4.33e-16	71.6
MS.gene059224.t1	AT5G18080	50.000	72	33	2	78	148	20	89	4.57e-16	69.7
MS.gene059224.t1	AT3G03820	53.731	67	30	1	83	148	28	94	4.63e-16	69.7
MS.gene059224.t1	AT3G20220	44.578	83	41	3	66	147	32	110	8.58e-16	69.7
MS.gene059224.t1	AT3G03850	46.250	80	40	3	70	148	14	91	1.11e-15	68.9
MS.gene059224.t1	AT4G34780	48.529	68	34	1	82	148	28	95	1.24e-15	68.9
MS.gene059224.t1	AT4G34770	50.000	72	33	3	82	152	35	104	1.57e-15	68.6
MS.gene059224.t1	AT5G18010	47.222	72	35	2	78	148	20	89	1.84e-15	68.2
MS.gene059224.t1	AT2G21220	44.000	75	37	2	76	148	32	103	2.12e-15	68.6
MS.gene059224.t1	AT4G38825	49.254	67	31	2	83	148	24	88	3.20e-15	67.4
MS.gene059224.t1	AT4G38840	49.254	67	31	2	82	147	33	97	3.29e-15	67.8
MS.gene059224.t1	AT5G20810	51.471	68	27	2	77	141	71	135	3.39e-15	69.3
MS.gene059224.t1	AT5G10990	44.186	86	42	2	80	162	39	121	4.57e-15	68.6
MS.gene059224.t1	AT5G18020	47.887	71	34	2	78	147	20	88	4.84e-15	67.0
MS.gene059224.t1	AT5G18050	48.571	70	33	2	80	148	22	89	5.43e-15	67.0
MS.gene059224.t1	AT5G20810	51.471	68	27	2	77	141	71	135	6.89e-15	69.3
MS.gene059224.t1	AT5G18060	49.254	67	31	2	83	148	25	89	2.67e-14	65.1
MS.gene059224.t1	AT1G56150	42.500	80	43	1	76	155	32	108	2.71e-14	65.5
MS.gene059224.t1	AT5G18030	45.833	72	36	2	78	148	18	87	2.95e-14	65.1
MS.gene059224.t1	AT3G03840	50.746	67	31	2	83	148	28	93	3.63e-14	65.1
MS.gene059224.t1	AT4G34760	44.776	67	34	1	82	148	43	106	4.72e-14	65.1
MS.gene059224.t1	AT1G75580	38.889	108	55	5	46	148	6	107	1.34e-13	63.9
MS.gene059224.t1	AT1G75590	49.206	63	29	1	80	142	40	99	2.00e-13	64.3
MS.gene059224.t1	AT2G21200	46.269	67	33	2	83	148	22	86	2.88e-13	62.4
MS.gene059224.t1	AT4G38860	44.737	76	36	3	76	148	32	104	3.29e-13	62.8
MS.gene059224.t1	AT4G34750	45.946	74	33	2	71	144	30	96	3.53e-13	63.9
MS.gene059224.t1	AT4G34750	45.946	74	33	2	71	144	30	96	3.53e-13	63.9
MS.gene059224.t1	AT4G13790	47.761	67	33	2	83	148	26	91	5.45e-13	61.6
MS.gene059224.t1	AT1G19830	43.284	67	35	1	82	148	48	111	1.57e-12	61.2
MS.gene059224.t1	AT3G12830	41.667	72	39	1	80	151	48	116	1.63e-12	61.6
MS.gene059224.t1	AT3G03830	45.714	70	37	1	80	148	22	91	1.70e-12	60.5
MS.gene059224.t1	AT1G19840	47.619	63	30	1	80	142	39	98	2.12e-12	61.6
MS.gene059224.t1	AT4G36110	46.774	62	30	1	80	141	38	96	3.29e-12	60.1
MS.gene059224.t1	AT5G66260	37.838	74	43	1	75	148	27	97	3.93e-12	59.7
MS.gene059224.t1	AT1G16510	42.857	70	37	1	82	151	54	120	5.43e-12	60.5
MS.gene059224.t1	AT2G24400	47.619	63	30	1	82	144	64	123	2.48e-11	59.3
MS.gene059224.t1	AT2G18010	49.091	55	25	1	82	136	48	99	2.98e-11	57.8
MS.gene059224.t1	AT4G31320	43.077	65	34	1	80	144	78	139	3.75e-11	59.3
MS.gene059224.t1	AT2G16580	33.663	101	56	2	48	148	18	107	5.02e-11	57.0
MS.gene059224.t1	AT2G37030	42.857	63	33	1	82	144	49	108	7.90e-11	57.0

Find 67 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACTTCAATCATCCTTTGTT+TGG	0.205259	4.1:+73130630	MS.gene059224:CDS
CGGTGAGTTTCGGCGGGTTT+TGG	0.248448	4.1:+73130596	MS.gene059224:CDS
ACTTCAATCATCCTTTGTTT+GGG	0.255039	4.1:+73130631	MS.gene059224:CDS
AAAGCAGGGTCGGGTAAATT+TGG	0.257203	4.1:+73130486	MS.gene059224:CDS
AAGTGATTCGGGTCAGTAAA+TGG	0.277611	4.1:+73130334	MS.gene059224:CDS
AATGGATATTCCGGCGATTT+CGG	0.281583	4.1:+73130352	MS.gene059224:CDS
AAAGAAGCTGAGAAAGAGTA+TGG	0.306967	4.1:+73130663	MS.gene059224:CDS
TATCCGACCCAGCTGCTATT+CGG	0.329644	4.1:-73130749	None:intergenic
GGTGAACCGCTTTGCTCAAC+CGG	0.334212	4.1:-73130531	None:intergenic
ATCCGACCCAGCTGCTATTC+GGG	0.371460	4.1:-73130748	None:intergenic
CGAGACGACGGTATCTTGTT+CGG	0.398391	4.1:-73130377	None:intergenic
TCGGGTTACCGAGTTTGAAC+GGG	0.414517	4.1:+73130719	MS.gene059224:CDS
GTCGGGTTACCGAGTTTGAA+CGG	0.425737	4.1:+73130718	MS.gene059224:CDS
GCTAGGGAAGAAAGTGATTC+GGG	0.437214	4.1:+73130323	MS.gene059224:CDS
AGCTAGGGAAGAAAGTGATT+CGG	0.439484	4.1:+73130322	MS.gene059224:CDS
CTTCCCTAGCTTGAATTGCA+TGG	0.441083	4.1:-73130310	None:intergenic
GTCGTCAAATGATTCTTCTC+CGG	0.445127	4.1:-73130408	None:intergenic
GAGACGACGGTATCTTGTTC+GGG	0.448535	4.1:-73130376	None:intergenic
GGGTCAGTAAATGGATATTC+CGG	0.450545	4.1:+73130343	MS.gene059224:CDS
TGGAATCACTATTCCTTGTC+GGG	0.453830	4.1:+73130701	MS.gene059224:CDS
GTGGAATCACTATTCCTTGT+CGG	0.471607	4.1:+73130700	MS.gene059224:CDS
GGGCATTTAGCGGTGTATGT+GGG	0.472930	4.1:+73130564	MS.gene059224:CDS
AAGTCTCTATGTTCAAAAGC+AGG	0.487934	4.1:+73130471	MS.gene059224:CDS
AATTTGGTTATTTGCCGATA+GGG	0.495078	4.1:+73130502	MS.gene059224:CDS
CCGTCGTCTCGGCGCCTCAC+CGG	0.497600	4.1:+73130389	MS.gene059224:CDS
GGGGCATTTAGCGGTGTATG+TGG	0.497853	4.1:+73130563	MS.gene059224:CDS
GCTAAATGCCCCTTCGGTAC+CGG	0.500069	4.1:-73130552	None:intergenic
TACACCGCTAAATGCCCCTT+CGG	0.500996	4.1:-73130558	None:intergenic
GTTAAGACCCGAATAGCAGC+TGG	0.501036	4.1:+73130741	MS.gene059224:CDS
TGGGTCGGATACGCGGAGAT+TGG	0.501712	4.1:+73130761	MS.gene059224:CDS
TGACGACAAAGCTTCTATCA+TGG	0.502757	4.1:+73130424	MS.gene059224:CDS
GACGACAAAGCTTCTATCAT+GGG	0.503605	4.1:+73130425	MS.gene059224:CDS
AAATTTGGTTATTTGCCGAT+AGG	0.508006	4.1:+73130501	MS.gene059224:CDS
ATAACCATGCAATTCAAGCT+AGG	0.509622	4.1:+73130306	None:intergenic
GGTCTTAACCCGTTCAAACT+CGG	0.514111	4.1:-73130727	None:intergenic
CTATGTTCAAAAGCAGGGTC+GGG	0.516192	4.1:+73130477	MS.gene059224:CDS
CTTCAATCATCCTTTGTTTG+GGG	0.517007	4.1:+73130632	MS.gene059224:CDS
AAGCGGTTCACCGGTACCGA+AGG	0.543995	4.1:+73130542	MS.gene059224:CDS
GGCATTTAGCGGTGTATGTG+GGG	0.555901	4.1:+73130565	MS.gene059224:CDS
AAAAGACGGTGAGTTTCGGC+GGG	0.560575	4.1:+73130590	MS.gene059224:CDS
TCTATGTTCAAAAGCAGGGT+CGG	0.560582	4.1:+73130476	MS.gene059224:CDS
TTGTTCGGGTCCGAAATCGC+CGG	0.561790	4.1:-73130362	None:intergenic
TAACCATGCAATTCAAGCTA+GGG	0.566394	4.1:+73130307	None:intergenic
GGTTGAGCAAAGCGGTTCAC+CGG	0.573801	4.1:+73130533	MS.gene059224:CDS
TTCAACAACTCCCCAAACAA+AGG	0.574136	4.1:-73130642	None:intergenic
TAGCAGCTGGGTCGGATACG+CGG	0.575304	4.1:+73130754	MS.gene059224:CDS
GACCCGAATAGCAGCTGGGT+CGG	0.582499	4.1:+73130746	MS.gene059224:CDS
AGCGGTTCACCGGTACCGAA+GGG	0.590068	4.1:+73130543	MS.gene059224:CDS
GGTACCGAAGGGGCATTTAG+CGG	0.599134	4.1:+73130554	MS.gene059224:CDS
CCGGTGAGGCGCCGAGACGA+CGG	0.603271	4.1:-73130389	None:intergenic
AAGAAGCTGAGAAAGAGTAT+GGG	0.603282	4.1:+73130664	MS.gene059224:CDS
ACCGCTTTGCTCAACCGGGT+CGG	0.604772	4.1:-73130526	None:intergenic
TCAACCGGGTCGGACCCTAT+CGG	0.605566	4.1:-73130516	None:intergenic
GTGAACCGCTTTGCTCAACC+GGG	0.606769	4.1:-73130530	None:intergenic
ACGACAAAGCTTCTATCATG+GGG	0.613722	4.1:+73130426	MS.gene059224:CDS
CGAACAAGATACCGTCGTCT+CGG	0.613951	4.1:+73130378	MS.gene059224:CDS
AGTCTCTATGTTCAAAAGCA+GGG	0.613958	4.1:+73130472	MS.gene059224:CDS
CGGATACGCGGAGATTGGCG+AGG	0.616210	4.1:+73130766	MS.gene059224:CDS
TCAAACTCGGTAACCCGACA+AGG	0.618391	4.1:-73130714	None:intergenic
CAAATGATTCTTCTCCGGTG+AGG	0.645717	4.1:-73130403	None:intergenic
TTTGCCGATAGGGTCCGACC+CGG	0.646098	4.1:+73130512	MS.gene059224:CDS
GGATGATTGAAGTAAACCAC+CGG	0.649322	4.1:-73130621	None:intergenic
GCGGTTCACCGGTACCGAAG+GGG	0.656345	4.1:+73130544	MS.gene059224:CDS
TTAAGACCCGAATAGCAGCT+GGG	0.660451	4.1:+73130742	MS.gene059224:CDS
ATACGCGGAGATTGGCGAGG+CGG	0.663865	4.1:+73130769	MS.gene059224:CDS
TCCGACCCGGTTGAGCAAAG+CGG	0.712572	4.1:+73130525	MS.gene059224:CDS
CGACAAAGCTTCTATCATGG+GGG	0.730867	4.1:+73130427	MS.gene059224:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAATTTGGTTATTTGCCGAT+AGG	+	chr4.1:73130501-73130520	MS.gene059224:CDS	30.0%
	AATTTGGTTATTTGCCGATA+GGG	+	chr4.1:73130502-73130521	MS.gene059224:CDS	30.0%
	TACTTCAATCATCCTTTGTT+TGG	+	chr4.1:73130630-73130649	MS.gene059224:CDS	30.0%
!	ACTTCAATCATCCTTTGTTT+GGG	+	chr4.1:73130631-73130650	MS.gene059224:CDS	30.0%
	AAAGAAGCTGAGAAAGAGTA+TGG	+	chr4.1:73130663-73130682	MS.gene059224:CDS	35.0%
	AAGTCTCTATGTTCAAAAGC+AGG	+	chr4.1:73130471-73130490	MS.gene059224:CDS	35.0%
	AGTCTCTATGTTCAAAAGCA+GGG	+	chr4.1:73130472-73130491	MS.gene059224:CDS	35.0%
!	AAGAAGCTGAGAAAGAGTAT+GGG	+	chr4.1:73130664-73130683	MS.gene059224:CDS	35.0%
!	CTTCAATCATCCTTTGTTTG+GGG	+	chr4.1:73130632-73130651	MS.gene059224:CDS	35.0%
	AATGGATATTCCGGCGATTT+CGG	+	chr4.1:73130352-73130371	MS.gene059224:CDS	40.0%
	ACGACAAAGCTTCTATCATG+GGG	+	chr4.1:73130426-73130445	MS.gene059224:CDS	40.0%
	AGCTAGGGAAGAAAGTGATT+CGG	+	chr4.1:73130322-73130341	MS.gene059224:CDS	40.0%
	GACGACAAAGCTTCTATCAT+GGG	+	chr4.1:73130425-73130444	MS.gene059224:CDS	40.0%
	GGATGATTGAAGTAAACCAC+CGG	-	chr4.1:73130624-73130643	None:intergenic	40.0%
	GGGTCAGTAAATGGATATTC+CGG	+	chr4.1:73130343-73130362	MS.gene059224:CDS	40.0%
	GTCGTCAAATGATTCTTCTC+CGG	-	chr4.1:73130411-73130430	None:intergenic	40.0%
	GTGGAATCACTATTCCTTGT+CGG	+	chr4.1:73130700-73130719	MS.gene059224:CDS	40.0%
	TCTATGTTCAAAAGCAGGGT+CGG	+	chr4.1:73130476-73130495	MS.gene059224:CDS	40.0%
	TGACGACAAAGCTTCTATCA+TGG	+	chr4.1:73130424-73130443	MS.gene059224:CDS	40.0%
	TGGAATCACTATTCCTTGTC+GGG	+	chr4.1:73130701-73130720	MS.gene059224:CDS	40.0%
	TTCAACAACTCCCCAAACAA+AGG	-	chr4.1:73130645-73130664	None:intergenic	40.0%
!	AAGTGATTCGGGTCAGTAAA+TGG	+	chr4.1:73130334-73130353	MS.gene059224:CDS	40.0%
!!	GAGTATGGGTTTTGTCATCA+AGG	+	chr4.1:73130678-73130697	MS.gene059224:CDS	40.0%
!!	TATGGGTTTTGTCATCAAGG+TGG	+	chr4.1:73130681-73130700	MS.gene059224:CDS	40.0%
	AAAAAGACGGTGAGTTTCGG+CGG	+	chr4.1:73130589-73130608	MS.gene059224:CDS	45.0%
	AAAGCAGGGTCGGGTAAATT+TGG	+	chr4.1:73130486-73130505	MS.gene059224:CDS	45.0%
	CAAATGATTCTTCTCCGGTG+AGG	-	chr4.1:73130406-73130425	None:intergenic	45.0%
	CGACAAAGCTTCTATCATGG+GGG	+	chr4.1:73130427-73130446	MS.gene059224:CDS	45.0%
	CTATGTTCAAAAGCAGGGTC+GGG	+	chr4.1:73130477-73130496	MS.gene059224:CDS	45.0%
	GCTAGGGAAGAAAGTGATTC+GGG	+	chr4.1:73130323-73130342	MS.gene059224:CDS	45.0%
	GGCAAAAAGACGGTGAGTTT+CGG	+	chr4.1:73130586-73130605	MS.gene059224:CDS	45.0%
	GGTCTTAACCCGTTCAAACT+CGG	-	chr4.1:73130730-73130749	None:intergenic	45.0%
	GTGTATGTGGGGCAAAAAGA+CGG	+	chr4.1:73130576-73130595	MS.gene059224:CDS	45.0%
	TTAAGACCCGAATAGCAGCT+GGG	+	chr4.1:73130742-73130761	MS.gene059224:CDS	45.0%
	AAAAGACGGTGAGTTTCGGC+GGG	+	chr4.1:73130590-73130609	MS.gene059224:CDS	50.0%
	CGAACAAGATACCGTCGTCT+CGG	+	chr4.1:73130378-73130397	MS.gene059224:CDS	50.0%
	CGAGACGACGGTATCTTGTT+CGG	-	chr4.1:73130380-73130399	None:intergenic	50.0%
	GAGACGACGGTATCTTGTTC+GGG	-	chr4.1:73130379-73130398	None:intergenic	50.0%
	GGCATTTAGCGGTGTATGTG+GGG	+	chr4.1:73130565-73130584	MS.gene059224:CDS	50.0%
	GGGCATTTAGCGGTGTATGT+GGG	+	chr4.1:73130564-73130583	MS.gene059224:CDS	50.0%
	GTCGGGTTACCGAGTTTGAA+CGG	+	chr4.1:73130718-73130737	MS.gene059224:CDS	50.0%
	GTTAAGACCCGAATAGCAGC+TGG	+	chr4.1:73130741-73130760	MS.gene059224:CDS	50.0%
	TACACCGCTAAATGCCCCTT+CGG	-	chr4.1:73130561-73130580	None:intergenic	50.0%
	TATCCGACCCAGCTGCTATT+CGG	-	chr4.1:73130752-73130771	None:intergenic	50.0%
	TCAAACTCGGTAACCCGACA+AGG	-	chr4.1:73130717-73130736	None:intergenic	50.0%
	TCGGGTTACCGAGTTTGAAC+GGG	+	chr4.1:73130719-73130738	MS.gene059224:CDS	50.0%
	ATCCGACCCAGCTGCTATTC+GGG	-	chr4.1:73130751-73130770	None:intergenic	55.0%
	GCTAAATGCCCCTTCGGTAC+CGG	-	chr4.1:73130555-73130574	None:intergenic	55.0%
	GGTGAACCGCTTTGCTCAAC+CGG	-	chr4.1:73130534-73130553	None:intergenic	55.0%
	GGTTGAGCAAAGCGGTTCAC+CGG	+	chr4.1:73130533-73130552	MS.gene059224:CDS	55.0%
	GTGAACCGCTTTGCTCAACC+GGG	-	chr4.1:73130533-73130552	None:intergenic	55.0%
	TTGTTCGGGTCCGAAATCGC+CGG	-	chr4.1:73130365-73130384	None:intergenic	55.0%
!	GGGGCATTTAGCGGTGTATG+TGG	+	chr4.1:73130563-73130582	MS.gene059224:CDS	55.0%
!!	GGTACCGAAGGGGCATTTAG+CGG	+	chr4.1:73130554-73130573	MS.gene059224:CDS	55.0%
	ACCGCTTTGCTCAACCGGGT+CGG	-	chr4.1:73130529-73130548	None:intergenic	60.0%
	ATACGCGGAGATTGGCGAGG+CGG	+	chr4.1:73130769-73130788	MS.gene059224:CDS	60.0%
	GACCCGAATAGCAGCTGGGT+CGG	+	chr4.1:73130746-73130765	MS.gene059224:CDS	60.0%
	TAGCAGCTGGGTCGGATACG+CGG	+	chr4.1:73130754-73130773	MS.gene059224:CDS	60.0%
	TCAACCGGGTCGGACCCTAT+CGG	-	chr4.1:73130519-73130538	None:intergenic	60.0%
	TCCGACCCGGTTGAGCAAAG+CGG	+	chr4.1:73130525-73130544	MS.gene059224:CDS	60.0%
	TGGGTCGGATACGCGGAGAT+TGG	+	chr4.1:73130761-73130780	MS.gene059224:CDS	60.0%
	TTTGCCGATAGGGTCCGACC+CGG	+	chr4.1:73130512-73130531	MS.gene059224:CDS	60.0%
!	AAGCGGTTCACCGGTACCGA+AGG	+	chr4.1:73130542-73130561	MS.gene059224:CDS	60.0%
!	AGCGGTTCACCGGTACCGAA+GGG	+	chr4.1:73130543-73130562	MS.gene059224:CDS	60.0%
!!	CGGTGAGTTTCGGCGGGTTT+TGG	+	chr4.1:73130596-73130615	MS.gene059224:CDS	60.0%
!!	GTTTCGGCGGGTTTTGGTGC+CGG	+	chr4.1:73130602-73130621	MS.gene059224:CDS	60.0%
	CGGATACGCGGAGATTGGCG+AGG	+	chr4.1:73130766-73130785	MS.gene059224:CDS	65.0%
!	GCGGTTCACCGGTACCGAAG+GGG	+	chr4.1:73130544-73130563	MS.gene059224:CDS	65.0%
!!!	TCGGCGGGTTTTGGTGCCGG+TGG	+	chr4.1:73130605-73130624	MS.gene059224:CDS	70.0%
	CCGTCGTCTCGGCGCCTCAC+CGG	+	chr4.1:73130389-73130408	MS.gene059224:CDS	75.0%
!!	CCGGTGAGGCGCCGAGACGA+CGG	-	chr4.1:73130392-73130411	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	73130312	73130809	73130312	ID=MS.gene059224
chr4.1	mRNA	73130312	73130809	73130312	ID=MS.gene059224.t1;Parent=MS.gene059224
chr4.1	exon	73130312	73130809	73130312	ID=MS.gene059224.t1.exon1;Parent=MS.gene059224.t1
chr4.1	CDS	73130312	73130809	73130312	ID=cds.MS.gene059224.t1;Parent=MS.gene059224.t1

Gene Sequence

>MS.gene059224

ATGCAATTCAAGCTAGGGAAGAAAGTGATTCGGGTCAGTAAATGGATATTCCGGCGATTTCGGACCCGAACAAGATACCGTCGTCTCGGCGCCTCACCGGAGAAGAATCATTTGACGACAAAGCTTCTATCATGGGGGAATAAGTTAACTACAAAAGCAAAGTCTCTATGTTCAAAAGCAGGGTCGGGTAAATTTGGTTATTTGCCGATAGGGTCCGACCCGGTTGAGCAAAGCGGTTCACCGGTACCGAAGGGGCATTTAGCGGTGTATGTGGGGCAAAAAGACGGTGAGTTTCGGCGGGTTTTGGTGCCGGTGGTTTACTTCAATCATCCTTTGTTTGGGGAGTTGTTGAAAGAAGCTGAGAAAGAGTATGGGTTTTGTCATCAAGGTGGAATCACTATTCCTTGTCGGGTTACCGAGTTTGAACGGGTTAAGACCCGAATAGCAGCTGGGTCGGATACGCGGAGATTGGCGAGGCGGAGACATTTTGACTGCTGA

Protein sequence

>MS.gene059224.t1

MQFKLGKKVIRVSKWIFRRFRTRTRYRRLGASPEKNHLTTKLLSWGNKLTTKAKSLCSKAGSGKFGYLPIGSDPVEQSGSPVPKGHLAVYVGQKDGEFRRVLVPVVYFNHPLFGELLKEAEKEYGFCHQGGITIPCRVTEFERVKTRIAAGSDTRRLARRRHFDC