Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46064.t1 | XP_003623166.1 | 97.4 | 117 | 3 | 0 | 1 | 117 | 1 | 117 | 1.40E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46064.t1 | G7L5L7 | 97.4 | 117 | 3 | 0 | 1 | 117 | 1 | 117 | 1.0e-58 | 235.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053776 | MS.gene46064 | 0.829865 | 4.05E-55 | -1.69E-46 |
| MS.gene055158 | MS.gene46064 | 0.835611 | 1.52E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46064.t1 | MTR_7g065210 | 97.436 | 117 | 3 | 0 | 1 | 117 | 1 | 117 | 3.73e-82 | 236 |
| MS.gene46064.t1 | MTR_7g065160 | 88.034 | 117 | 14 | 0 | 1 | 117 | 1 | 117 | 1.34e-74 | 217 |
| MS.gene46064.t1 | MTR_8g070040 | 51.515 | 99 | 46 | 2 | 20 | 117 | 23 | 120 | 1.11e-25 | 93.6 |
| MS.gene46064.t1 | MTR_8g070015 | 47.826 | 92 | 46 | 2 | 20 | 110 | 23 | 113 | 4.82e-20 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46064.t1 | AT3G53980 | 68.421 | 95 | 29 | 1 | 23 | 117 | 21 | 114 | 1.31e-44 | 141 |
| MS.gene46064.t1 | AT3G53980 | 68.421 | 95 | 29 | 1 | 23 | 117 | 21 | 114 | 1.31e-44 | 141 |
| MS.gene46064.t1 | AT5G05960 | 58.772 | 114 | 40 | 2 | 4 | 117 | 10 | 116 | 1.41e-42 | 136 |
| MS.gene46064.t1 | AT2G37870 | 45.370 | 108 | 57 | 2 | 11 | 117 | 9 | 115 | 9.88e-26 | 93.6 |
Find 21 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTAAATGCTTCCATATC+TGG | 0.265044 | 7.2:+42706488 | None:intergenic |
| TAGCAAAGTTACAGCGTTTA+GGG | 0.295883 | 7.2:+42706303 | None:intergenic |
| TTAGCAAAGTTACAGCGTTT+AGG | 0.343174 | 7.2:+42706302 | None:intergenic |
| CGTCCGCACTTATAACCAAT+GGG | 0.457526 | 7.2:+42706275 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.462464 | 7.2:-42706353 | MS.gene46064:CDS |
| AAGGGGTACTACACCAGATA+TGG | 0.470603 | 7.2:-42706501 | MS.gene46064:CDS |
| GGATAGTAACAGCAATTTGA+GGG | 0.472152 | 7.2:+42706324 | None:intergenic |
| ATGAAGATGCTGTGCCTAGT+TGG | 0.489536 | 7.2:-42706578 | MS.gene46064:CDS |
| GGGATAGTAACAGCAATTTG+AGG | 0.508534 | 7.2:+42706323 | None:intergenic |
| ACGTCCGCACTTATAACCAA+TGG | 0.512832 | 7.2:+42706274 | None:intergenic |
| GTTGAAAGTGCAGGTGAATG+TGG | 0.515102 | 7.2:-42706524 | MS.gene46064:CDS |
| AACTTTGCTAATCGCCCCAT+TGG | 0.524057 | 7.2:-42706290 | MS.gene46064:CDS |
| TCTTGTGCTGCACTTGCACA+AGG | 0.541464 | 7.2:+42706461 | None:intergenic |
| AAAGTGCAGGTGAATGTGGA+AGG | 0.549386 | 7.2:-42706520 | MS.gene46064:CDS |
| AAAACTGCACAAAGACAACT+TGG | 0.590774 | 7.2:+42706380 | None:intergenic |
| ATTCATAGAGTTGAAAGTGC+AGG | 0.591870 | 7.2:-42706533 | MS.gene46064:CDS |
| CAAGAACTACAAAACCAACT+AGG | 0.604153 | 7.2:+42706564 | None:intergenic |
| CGCCCCATTGGTTATAAGTG+CGG | 0.627824 | 7.2:-42706278 | MS.gene46064:CDS |
| AGTGCAGGTGAATGTGGAAG+GGG | 0.630405 | 7.2:-42706518 | MS.gene46064:CDS |
| AAGTGCAGGTGAATGTGGAA+GGG | 0.641454 | 7.2:-42706519 | MS.gene46064:CDS |
| GTCCGCACTTATAACCAATG+GGG | 0.757650 | 7.2:+42706276 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGAGAAATATATTAATAT+AGG | - | chr7.2:42706086-42706105 | MS.gene46064:intron | 10.0% |
| !! | TATATTAATATAGGAAAAAA+AGG | - | chr7.2:42706095-42706114 | MS.gene46064:intron | 10.0% |
| !!! | TTTCATTACATTATTTTTTT+TGG | + | chr7.2:42706451-42706470 | None:intergenic | 10.0% |
| !! | AAAAATAAAAAAATAACACG+TGG | - | chr7.2:42706262-42706281 | MS.gene46064:intron | 15.0% |
| !! | AAGAATAAATATAAACGAAA+TGG | + | chr7.2:42705960-42705979 | None:intergenic | 15.0% |
| !!! | ATTTTGTAGTTTTTTTTCTT+AGG | + | chr7.2:42706172-42706191 | None:intergenic | 15.0% |
| !!! | TTTTATTTTTAAATTCATGC+AGG | + | chr7.2:42706252-42706271 | None:intergenic | 15.0% |
| !!! | TTTTGTAGTTTTTTTTCTTA+GGG | + | chr7.2:42706171-42706190 | None:intergenic | 15.0% |
| !!! | TTTTGTAGTTTTTTTTTCTT+AGG | - | chr7.2:42706375-42706394 | MS.gene46064:CDS | 15.0% |
| !! | AACAACAAAAAAATGTGAAA+CGG | - | chr7.2:42706471-42706490 | MS.gene46064:CDS | 20.0% |
| !!! | CTATTTGTTAATTTTCTTGA+TGG | - | chr7.2:42706532-42706551 | MS.gene46064:CDS | 20.0% |
| !!! | TTTGTAGTTTTTTTTCTTAG+GGG | + | chr7.2:42706170-42706189 | None:intergenic | 20.0% |
| !!! | TGTAGTTTTTTTTTCTTAGG+AGG | - | chr7.2:42706378-42706397 | MS.gene46064:CDS | 25.0% |
| !!! | TTGTAGTTTTTTTTCTTAGG+GGG | + | chr7.2:42706169-42706188 | None:intergenic | 25.0% |
| GACAACTTAAAAGAAACTGA+GGG | + | chr7.2:42705922-42705941 | None:intergenic | 30.0% | |
| TGTTACAGTTGCAAAACTTA+GGG | + | chr7.2:42706199-42706218 | None:intergenic | 30.0% | |
| ! | TTTTGCAACTGTAACAGTTT+TGG | - | chr7.2:42706203-42706222 | MS.gene46064:intron | 30.0% |
| AAAACTGCACAAAGACAACT+TGG | + | chr7.2:42705778-42705797 | None:intergenic | 35.0% | |
| AGCTTAAATGCTTCCATATC+TGG | + | chr7.2:42705670-42705689 | None:intergenic | 35.0% | |
| CAAGAACTACAAAACCAACT+AGG | + | chr7.2:42705594-42705613 | None:intergenic | 35.0% | |
| CGACAACTTAAAAGAAACTG+AGG | + | chr7.2:42705923-42705942 | None:intergenic | 35.0% | |
| CTAAAATCACAACTTCGTGA+AGG | + | chr7.2:42706146-42706165 | None:intergenic | 35.0% | |
| CTGTTACAGTTGCAAAACTT+AGG | + | chr7.2:42706200-42706219 | None:intergenic | 35.0% | |
| GGATAGTAACAGCAATTTGA+GGG | + | chr7.2:42705834-42705853 | None:intergenic | 35.0% | |
| GTTACAGTTGCAAAACTTAG+GGG | + | chr7.2:42706198-42706217 | None:intergenic | 35.0% | |
| TAGCAAAGTTACAGCGTTTA+GGG | + | chr7.2:42705855-42705874 | None:intergenic | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | - | chr7.2:42705802-42705821 | MS.gene46064:intron | 35.0% | |
| TCATGCAGGTGCAAAAAAAT+TGG | + | chr7.2:42706238-42706257 | None:intergenic | 35.0% | |
| TGTGCTCAGACTAAAAAACT+TGG | - | chr7.2:42705745-42705764 | MS.gene46064:intron | 35.0% | |
| TTACAGTTGCAAAACTTAGG+GGG | + | chr7.2:42706197-42706216 | None:intergenic | 35.0% | |
| TTAGCAAAGTTACAGCGTTT+AGG | + | chr7.2:42705856-42705875 | None:intergenic | 35.0% | |
| ! | AATCGCAACTTTGGAAAGTT+AGG | - | chr7.2:42706405-42706424 | MS.gene46064:CDS | 35.0% |
| ! | ACTTTCCAAAGTTGCGATTT+TGG | + | chr7.2:42706404-42706423 | None:intergenic | 35.0% |
| ! | ATCGCAACTTTGGAAAGTTA+GGG | - | chr7.2:42706406-42706425 | MS.gene46064:CDS | 35.0% |
| ! | ATTCATAGAGTTGAAAGTGC+AGG | - | chr7.2:42705622-42705641 | MS.gene46064:intron | 35.0% |
| ! | GTTTTGTAGTTCTTGTTGCA+GGG | - | chr7.2:42705599-42705618 | MS.gene46064:intron | 35.0% |
| ATCACAACTTCGTGAAGGTT+AGG | + | chr7.2:42706141-42706160 | None:intergenic | 40.0% | |
| GGGATAGTAACAGCAATTTG+AGG | + | chr7.2:42705835-42705854 | None:intergenic | 40.0% | |
| TCACAACTTCGTGAAGGTTA+GGG | + | chr7.2:42706140-42706159 | None:intergenic | 40.0% | |
| ! | AGGTGCAAAAAAATTGGCCT+TGG | + | chr7.2:42706232-42706251 | None:intergenic | 40.0% |
| !! | GGTGCAAAAAAATTGGCCTT+GGG | + | chr7.2:42706231-42706250 | None:intergenic | 40.0% |
| !! | GGTTTTGTAGTTCTTGTTGC+AGG | - | chr7.2:42705598-42705617 | MS.gene46064:intron | 40.0% |
| !! | GTGCAAAAAAATTGGCCTTG+GGG | + | chr7.2:42706230-42706249 | None:intergenic | 40.0% |
| !! | TGCAAAAAAATTGGCCTTGG+GGG | + | chr7.2:42706229-42706248 | None:intergenic | 40.0% |
| AAAGTGCAGGTGAATGTGGA+AGG | - | chr7.2:42705635-42705654 | MS.gene46064:intron | 45.0% | |
| AACACGTGGCACCTCATATA+GGG | - | chr7.2:42706276-42706295 | MS.gene46064:CDS | 45.0% | |
| AACTTTGCTAATCGCCCCAT+TGG | - | chr7.2:42705865-42705884 | MS.gene46064:intron | 45.0% | |
| AAGTGCAGGTGAATGTGGAA+GGG | - | chr7.2:42705636-42705655 | MS.gene46064:intron | 45.0% | |
| ACAACTTCGTGAAGGTTAGG+GGG | + | chr7.2:42706138-42706157 | None:intergenic | 45.0% | |
| ACGTCCGCACTTATAACCAA+TGG | + | chr7.2:42705884-42705903 | None:intergenic | 45.0% | |
| AGCGTAGACCGAGTCAAAAT+TGG | - | chr7.2:42706308-42706327 | MS.gene46064:CDS | 45.0% | |
| ATGAAGATGCTGTGCCTAGT+TGG | - | chr7.2:42705577-42705596 | MS.gene46064:intron | 45.0% | |
| CACAACTTCGTGAAGGTTAG+GGG | + | chr7.2:42706139-42706158 | None:intergenic | 45.0% | |
| CGTCCGCACTTATAACCAAT+GGG | + | chr7.2:42705883-42705902 | None:intergenic | 45.0% | |
| GTCCGCACTTATAACCAATG+GGG | + | chr7.2:42705882-42705901 | None:intergenic | 45.0% | |
| TAACACGTGGCACCTCATAT+AGG | - | chr7.2:42706275-42706294 | MS.gene46064:intron | 45.0% | |
| ! | GTTGAAAGTGCAGGTGAATG+TGG | - | chr7.2:42705631-42705650 | MS.gene46064:intron | 45.0% |
| !! | AAGGGGTACTACACCAGATA+TGG | - | chr7.2:42705654-42705673 | MS.gene46064:intron | 45.0% |
| !! | ACCGAGTCAAAATTGGCCTT+GGG | - | chr7.2:42706315-42706334 | MS.gene46064:CDS | 45.0% |
| AGTGCAGGTGAATGTGGAAG+GGG | - | chr7.2:42705637-42705656 | MS.gene46064:intron | 50.0% | |
| CCACCGGAGCAAAACTTAGA+GGG | - | chr7.2:42706349-42706368 | MS.gene46064:CDS | 50.0% | |
| CTGATGTGGCACCCTATATG+AGG | + | chr7.2:42706290-42706309 | None:intergenic | 50.0% | |
| GGAGGCCAAAATCGCAACTT+TGG | - | chr7.2:42706396-42706415 | MS.gene46064:CDS | 50.0% | |
| TCTTGTGCTGCACTTGCACA+AGG | + | chr7.2:42705697-42705716 | None:intergenic | 50.0% | |
| ! | CGCCCCATTGGTTATAAGTG+CGG | - | chr7.2:42705877-42705896 | MS.gene46064:intron | 50.0% |
| ! | GACCGAGTCAAAATTGGCCT+TGG | - | chr7.2:42706314-42706333 | MS.gene46064:CDS | 50.0% |
| ! | TTTTGCTCCGGTGGCAGTTT+TGG | + | chr7.2:42706343-42706362 | None:intergenic | 50.0% |
| !! | CCGAGTCAAAATTGGCCTTG+GGG | - | chr7.2:42706316-42706335 | MS.gene46064:CDS | 50.0% |
| !! | CGAGTCAAAATTGGCCTTGG+GGG | - | chr7.2:42706317-42706336 | MS.gene46064:CDS | 50.0% |
| !! | TGTAACAGTTTTGGCCCCCA+AGG | - | chr7.2:42706212-42706231 | MS.gene46064:intron | 50.0% |
| !!! | CCCCAAGGCCAATTTTGACT+CGG | + | chr7.2:42706319-42706338 | None:intergenic | 50.0% |
| CCGGAGCAAAACTTAGAGGG+CGG | - | chr7.2:42706352-42706371 | MS.gene46064:CDS | 55.0% | |
| GCCACCGGAGCAAAACTTAG+AGG | - | chr7.2:42706348-42706367 | MS.gene46064:CDS | 55.0% | |
| ! | CCCTCTAAGTTTTGCTCCGG+TGG | + | chr7.2:42706352-42706371 | None:intergenic | 55.0% |
| ! | CCGCCCTCTAAGTTTTGCTC+CGG | + | chr7.2:42706355-42706374 | None:intergenic | 55.0% |
| !! | TGACTCGGTCTACGCTGATG+TGG | + | chr7.2:42706304-42706323 | None:intergenic | 55.0% |
| TTGGGGGCCAAAACTGCCAC+CGG | - | chr7.2:42706333-42706352 | MS.gene46064:CDS | 60.0% | |
| !! | GGTGGCAGTTTTGGCCCCCA+AGG | + | chr7.2:42706334-42706353 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 42705565 | 42706612 | 42705565 | ID=MS.gene46064 |
| chr7.2 | mRNA | 42705565 | 42706612 | 42705565 | ID=MS.gene46064.t1;Parent=MS.gene46064 |
| chr7.2 | exon | 42706276 | 42706612 | 42706276 | ID=MS.gene46064.t1.exon1;Parent=MS.gene46064.t1 |
| chr7.2 | CDS | 42706276 | 42706612 | 42706276 | ID=cds.MS.gene46064.t1;Parent=MS.gene46064.t1 |
| chr7.2 | exon | 42705565 | 42705581 | 42705565 | ID=MS.gene46064.t1.exon2;Parent=MS.gene46064.t1 |
| chr7.2 | CDS | 42705565 | 42705581 | 42705565 | ID=cds.MS.gene46064.t1;Parent=MS.gene46064.t1 |
| Gene Sequence |
| Protein sequence |
