AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055158


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055158.t1 MTR_7g065210 94.872 117 6 0 1 117 1 117 3.66e-80 231
MS.gene055158.t1 MTR_7g065160 90.598 117 11 0 1 117 1 117 4.46e-76 220
MS.gene055158.t1 MTR_8g070040 51.515 99 46 2 20 117 23 120 1.60e-25 93.2
MS.gene055158.t1 MTR_8g070015 47.826 92 46 2 20 110 23 113 7.05e-20 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055158.t1 AT3G53980 68.421 95 29 1 23 117 21 114 1.22e-44 141
MS.gene055158.t1 AT3G53980 68.421 95 29 1 23 117 21 114 1.22e-44 141
MS.gene055158.t1 AT5G05960 58.772 114 40 2 4 117 10 116 7.24e-43 137
MS.gene055158.t1 AT2G37870 45.370 108 57 2 11 117 9 115 2.47e-25 92.4

Find 24 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTAAATGCTTCCATATC+TGG 0.265044 7.4:-39462073 None:intergenic
TAGCAAAGTTGCAGCGTTTA+GGG 0.296288 7.4:-39462258 None:intergenic
TTAGCAAAGTTGCAGCGTTT+AGG 0.351023 7.4:-39462259 None:intergenic
GGTATTGCAGTTCTTGTTGC+AGG 0.361788 7.4:+39462004 MS.gene055158:CDS
CGTCCACACTTATAACCAAT+GGG 0.425846 7.4:-39462286 None:intergenic
TCAAACGTTGCTAAAATGTC+TGG 0.462464 7.4:+39462208 MS.gene055158:CDS
AAGGGGTACTACACCAGATA+TGG 0.470603 7.4:+39462060 MS.gene055158:CDS
GGATAGTAACAGCAATTTGA+GGG 0.472152 7.4:-39462237 None:intergenic
GGGATAGTAACAGCAATTTG+AGG 0.508534 7.4:-39462238 None:intergenic
GTTGAAAGTGCAGGTGAATG+TGG 0.515102 7.4:+39462037 MS.gene055158:CDS
AACTTTGCTAATCGCCCCAT+TGG 0.518869 7.4:+39462271 MS.gene055158:CDS
ACGTCCACACTTATAACCAA+TGG 0.527913 7.4:-39462287 None:intergenic
CGAGGCACAACAATTTCATA+AGG 0.539901 7.4:-39461979 None:intergenic
TCTTGTGCTGCACTTGCACA+AGG 0.541464 7.4:-39462100 None:intergenic
AAAGTGCAGGTGAATGTGGA+AGG 0.549386 7.4:+39462041 MS.gene055158:CDS
ATGAAATTGTTGTGCCTCGT+TGG 0.557778 7.4:+39461983 MS.gene055158:CDS
CGCCCCATTGGTTATAAGTG+TGG 0.571913 7.4:+39462283 MS.gene055158:CDS
ATTCATAGAGTTGAAAGTGC+AGG 0.578390 7.4:+39462028 MS.gene055158:CDS
AAAACTGCACAAAGACAACT+TGG 0.590774 7.4:-39462181 None:intergenic
TGTGCTCAGACTAAGAAACT+TGG 0.591888 7.4:+39462151 MS.gene055158:CDS
AGTGCAGGTGAATGTGGAAG+GGG 0.630405 7.4:+39462043 MS.gene055158:CDS
AAGTGCAGGTGAATGTGGAA+GGG 0.641454 7.4:+39462042 MS.gene055158:CDS
CAAGAACTGCAATACCAACG+AGG 0.744882 7.4:-39461997 None:intergenic
GTCCACACTTATAACCAATG+GGG 0.748420 7.4:-39462285 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAACTTTTATATGAAAA+CGG - chr7.4:39462349-39462368 None:intergenic 10.0%
!!! TTTTAACAATAAATTTTCTT+AGG - chr7.4:39462476-39462495 None:intergenic 10.0%
!! ATGTAACATTAATAATTGAT+AGG + chr7.4:39462401-39462420 MS.gene055158:intron 15.0%
!!! GTTTTCATATAAAAGTTATT+TGG + chr7.4:39462348-39462367 MS.gene055158:intron 15.0%
!! AATGAGATTATAGATAGTTA+AGG + chr7.4:39462427-39462446 MS.gene055158:intron 20.0%
!! ATGAGATTATAGATAGTTAA+GGG + chr7.4:39462428-39462447 MS.gene055158:intron 20.0%
! TTGTTAAAATCTTCCCTTTA+TGG + chr7.4:39462487-39462506 MS.gene055158:intron 25.0%
!! CTATTTGTCAATTTTCTTGA+TGG + chr7.4:39462586-39462605 MS.gene055158:intron 25.0%
!!! TAGATAGTTAAGGGTATTAT+AGG + chr7.4:39462437-39462456 MS.gene055158:intron 25.0%
TGCCTAAGAAAAATGTGAAA+CGG + chr7.4:39462534-39462553 MS.gene055158:intron 30.0%
! TACCGTTTCACATTTTTCTT+AGG - chr7.4:39462539-39462558 None:intergenic 30.0%
!! CTAGTTTCTAGTTCCATAAA+GGG - chr7.4:39462503-39462522 None:intergenic 30.0%
!! TCTAGTTTCTAGTTCCATAA+AGG - chr7.4:39462504-39462523 None:intergenic 30.0%
AAAACTGCACAAAGACAACT+TGG - chr7.4:39462184-39462203 None:intergenic 35.0%
AGCTTAAATGCTTCCATATC+TGG - chr7.4:39462076-39462095 None:intergenic 35.0%
GGATAGTAACAGCAATTTGA+GGG - chr7.4:39462240-39462259 None:intergenic 35.0%
TAAGTAACTAACAAGTCCCT+CGG + chr7.4:39462309-39462328 MS.gene055158:intron 35.0%
TCAAACGTTGCTAAAATGTC+TGG + chr7.4:39462208-39462227 MS.gene055158:CDS 35.0%
! ATTCATAGAGTTGAAAGTGC+AGG + chr7.4:39462028-39462047 MS.gene055158:CDS 35.0%
ACGTCCACACTTATAACCAA+TGG - chr7.4:39462290-39462309 None:intergenic 40.0%
ATGAAATTGTTGTGCCTCGT+TGG + chr7.4:39461983-39462002 MS.gene055158:CDS 40.0%
CGAGGCACAACAATTTCATA+AGG - chr7.4:39461982-39462001 None:intergenic 40.0%
CGTCCACACTTATAACCAAT+GGG - chr7.4:39462289-39462308 None:intergenic 40.0%
GGCAACTTAAAAGAAACCGA+GGG - chr7.4:39462328-39462347 None:intergenic 40.0%
GGGATAGTAACAGCAATTTG+AGG - chr7.4:39462241-39462260 None:intergenic 40.0%
GTCCACACTTATAACCAATG+GGG - chr7.4:39462288-39462307 None:intergenic 40.0%
TAGCAAAGTTGCAGCGTTTA+GGG - chr7.4:39462261-39462280 None:intergenic 40.0%
TGTGCTCAGACTAAGAAACT+TGG + chr7.4:39462151-39462170 MS.gene055158:CDS 40.0%
TTAGCAAAGTTGCAGCGTTT+AGG - chr7.4:39462262-39462281 None:intergenic 40.0%
AAAGTGCAGGTGAATGTGGA+AGG + chr7.4:39462041-39462060 MS.gene055158:CDS 45.0%
AACTTTGCTAATCGCCCCAT+TGG + chr7.4:39462271-39462290 MS.gene055158:CDS 45.0%
AAGTGCAGGTGAATGTGGAA+GGG + chr7.4:39462042-39462061 MS.gene055158:CDS 45.0%
CAAGAACTGCAATACCAACG+AGG - chr7.4:39462000-39462019 None:intergenic 45.0%
CGGCAACTTAAAAGAAACCG+AGG - chr7.4:39462329-39462348 None:intergenic 45.0%
GGTATTGCAGTTCTTGTTGC+AGG + chr7.4:39462004-39462023 MS.gene055158:CDS 45.0%
! GTTGAAAGTGCAGGTGAATG+TGG + chr7.4:39462037-39462056 MS.gene055158:CDS 45.0%
!! AAGGGGTACTACACCAGATA+TGG + chr7.4:39462060-39462079 MS.gene055158:CDS 45.0%
AGTGCAGGTGAATGTGGAAG+GGG + chr7.4:39462043-39462062 MS.gene055158:CDS 50.0%
TCTTGTGCTGCACTTGCACA+AGG - chr7.4:39462103-39462122 None:intergenic 50.0%
! CGCCCCATTGGTTATAAGTG+TGG + chr7.4:39462283-39462302 MS.gene055158:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 39461971 39462666 39461971 ID=MS.gene055158
chr7.4 mRNA 39461971 39462666 39461971 ID=MS.gene055158.t1;Parent=MS.gene055158
chr7.4 exon 39461971 39462307 39461971 ID=MS.gene055158.t1.exon1;Parent=MS.gene055158.t1
chr7.4 CDS 39461971 39462307 39461971 ID=cds.MS.gene055158.t1;Parent=MS.gene055158.t1
chr7.4 exon 39462650 39462666 39462650 ID=MS.gene055158.t1.exon2;Parent=MS.gene055158.t1
chr7.4 CDS 39462650 39462666 39462650 ID=cds.MS.gene055158.t1;Parent=MS.gene055158.t1
Gene Sequence

>MS.gene055158

ATGGAGGGCCTTATGAAATTGTTGTGCCTCGTTGGTATTGCAGTTCTTGTTGCAGGAATTCATAGAGTTGAAAGTGCAGGTGAATGTGGAAGGGGTACTACACCAGATATGGAAGCATTTAAGCTTGCTCCTTGTGCAAGTGCAGCACAAGATGAAAATGCTAGTGTCTCACAAAGTTGTTGTGCTCAGACTAAGAAACTTGGTCAGAATCCAAGTTGTCTTTGTGCAGTTTTGCTCTCAAACGTTGCTAAAATGTCTGGTGTTAACCCTCAAATTGCTGTTACTATCCCTAAACGCTGCAACTTTGCTAATCGCCCCATTGGTTATAAGTGTGGACGTAAGTAACTAACAAGTCCCTCGGTTTCTTTTAAGTTGCCGTTTTCATATAAAAGTTATTTGGTTTATATTTATTTCTTGTTTTCAAAGTTTAATGTAACATTAATAATTGATAGGTGAAATGAGATTATAGATAGTTAAGGGTATTATAGGAAAAAAAATTATGCCTAAGAAAATTTATTGTTAAAATCTTCCCTTTATGGAACTAGAAACTAGAAACATATTTATGCCTAAGAAAAATGTGAAACGGTATGATACTAGTAATTTTTTTTTTCTTTTCTATTTGTCAATTTTCTTGATGGATCAATATACTAACTACTCTTATAAAATTCTGATTTTGCAGCTTACACTCTTCCTTGA

Protein sequence

>MS.gene055158.t1

MEGLMKLLCLVGIAVLVAGIHRVESAGECGRGTTPDMEAFKLAPCASAAQDENASVSQSCCAQTKKLGQNPSCLCAVLLSNVAKMSGVNPQIAVTIPKRCNFANRPIGYKCGPYTLP