Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46157.t1 | GAU38796.1 | 85.1 | 134 | 14 | 2 | 1 | 128 | 1 | 134 | 2.50E-53 | 218 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46157.t1 | A0A2Z6N4K6 | 85.1 | 134 | 14 | 2 | 1 | 128 | 1 | 134 | 1.8e-53 | 218.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene061049 | MS.gene46157 | 0.810848 | 9.40E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46157.t1 | MTR_7g062470 | 91.603 | 131 | 8 | 1 | 1 | 128 | 1 | 131 | 2.43e-69 | 204 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46157.t1 | AT5G02160 | 68.376 | 117 | 36 | 1 | 13 | 128 | 12 | 128 | 2.20e-46 | 146 |
Find 26 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTTGTTGCTGGTGCTAT+TGG | 0.295597 | 7.2:-40528332 | MS.gene46157:CDS |
| GGCACTATTTATCTTGTTGC+TGG | 0.299445 | 7.2:-40528341 | MS.gene46157:CDS |
| AAATGAGAGTTTAGTGTTGT+TGG | 0.340680 | 7.2:+40528460 | None:intergenic |
| GCTATTGGAATTGCTCTTGT+TGG | 0.368974 | 7.2:-40528317 | MS.gene46157:CDS |
| AACAACACTAAACTCTCATT+TGG | 0.372290 | 7.2:-40528458 | MS.gene46157:CDS |
| TTGTGCTCGTTGTGATGGTT+TGG | 0.405035 | 7.2:-40526853 | MS.gene46157:CDS |
| AGCATTTCTATTTGTCAACT+TGG | 0.409660 | 7.2:+40526820 | None:intergenic |
| TGGAGCAGGGTTTGTTAGAA+AGG | 0.411059 | 7.2:-40526928 | MS.gene46157:CDS |
| TCTGTTATTGTTTCAGCAGT+TGG | 0.416895 | 7.2:-40528380 | MS.gene46157:CDS |
| AACCCTGCTCCATCACAATC+AGG | 0.417979 | 7.2:+40526939 | None:intergenic |
| ATTGGGAATGATTGTGTTCA+AGG | 0.420585 | 7.2:+40528493 | None:intergenic |
| TGTGCTCGTTGTGATGGTTT+GGG | 0.434742 | 7.2:-40526852 | MS.gene46157:CDS |
| GCAGGGTTTGTTAGAAAGGG+TGG | 0.449785 | 7.2:-40526924 | MS.gene46157:CDS |
| GAGAGCAAATGCAGCAAGAA+AGG | 0.478276 | 7.2:-40526892 | MS.gene46157:CDS |
| GTTGTTGGAAATTGTAACAT+TGG | 0.483393 | 7.2:+40528475 | None:intergenic |
| AACACTAAACTCTCATTTGG+AGG | 0.496202 | 7.2:-40528455 | MS.gene46157:CDS |
| GGAGCAGGGTTTGTTAGAAA+GGG | 0.500570 | 7.2:-40526927 | MS.gene46157:CDS |
| TGCCCTGATTGTGATGGAGC+AGG | 0.517909 | 7.2:-40526942 | MS.gene46157:CDS |
| TTGTTGGAAATTGTAACATT+GGG | 0.522805 | 7.2:+40528476 | None:intergenic |
| ATTGTTTGTGCTCGTTGTGA+TGG | 0.530028 | 7.2:-40526858 | MS.gene46157:CDS |
| AATGATTGTGTTCAAGGTCA+TGG | 0.560862 | 7.2:+40528499 | None:intergenic |
| TCTTTACGAGAGAAAATGAC+AGG | 0.592838 | 7.2:+40528284 | None:intergenic |
| GTTGGAGATGTATCTTCTGA+TGG | 0.593310 | 7.2:-40528362 | MS.gene46157:CDS |
| ACCCTGCTCCATCACAATCA+GGG | 0.608104 | 7.2:+40526940 | None:intergenic |
| GCCCTGATTGTGATGGAGCA+GGG | 0.608764 | 7.2:-40526941 | MS.gene46157:CDS |
| ATCAGATGCCCTGATTGTGA+TGG | 0.654871 | 7.2:-40526948 | MS.gene46157:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTATAAATATTTATAT+TGG | + | chr7.2:40527682-40527701 | None:intergenic | 0.0% |
| !! | AAATTATAAATATTTATATT+GGG | + | chr7.2:40527681-40527700 | None:intergenic | 0.0% |
| !! | AATAATAATCTTAATAATAG+TGG | - | chr7.2:40527706-40527725 | MS.gene46157:intron | 10.0% |
| !!! | TAGTTTTTATTTTTTTTGAA+AGG | - | chr7.2:40527506-40527525 | MS.gene46157:intron | 10.0% |
| !! | AAAAATAGGTTTATAATTCT+TGG | + | chr7.2:40528069-40528088 | None:intergenic | 15.0% |
| !! | ATAAAAACTATACTTATCTA+TGG | + | chr7.2:40527496-40527515 | None:intergenic | 15.0% |
| !! | TTATAAATAGAAATTAGAGA+TGG | - | chr7.2:40527316-40527335 | MS.gene46157:intron | 15.0% |
| !!! | AATTAGTTTTTTAATCTACT+TGG | - | chr7.2:40528258-40528277 | MS.gene46157:intron | 15.0% |
| !!! | ATTAGTTTTTTAATCTACTT+GGG | - | chr7.2:40528259-40528278 | MS.gene46157:intron | 15.0% |
| !!! | ATTTTAAAACAAAACATGTT+TGG | + | chr7.2:40528158-40528177 | None:intergenic | 15.0% |
| !! | AACATAAGATTAAGAATTCA+AGG | + | chr7.2:40528223-40528242 | None:intergenic | 20.0% |
| !! | ATGGTTAAAACATGTAAAAT+AGG | - | chr7.2:40528317-40528336 | MS.gene46157:CDS | 20.0% |
| !! | CCTAGAATAAAAAAAAGTTT+AGG | + | chr7.2:40527244-40527263 | None:intergenic | 20.0% |
| !! | TAAAAAACAAAACCTGATTT+CGG | - | chr7.2:40527572-40527591 | MS.gene46157:intron | 20.0% |
| !! | TGGATAAAATGAATTTGAAT+TGG | + | chr7.2:40528138-40528157 | None:intergenic | 20.0% |
| !!! | AATTTTTGTATAGGCATTTT+TGG | - | chr7.2:40527267-40527286 | MS.gene46157:intron | 20.0% |
| !!! | CCTAAACTTTTTTTTATTCT+AGG | - | chr7.2:40527241-40527260 | MS.gene46157:intron | 20.0% |
| !!! | CTTTGTTATTTTCATTTTCA+TGG | - | chr7.2:40527089-40527108 | MS.gene46157:intron | 20.0% |
| !!! | GAATTATAAACCTATTTTTG+TGG | - | chr7.2:40528070-40528089 | MS.gene46157:intron | 20.0% |
| !!! | TGATTTTTGTCTATATAACT+AGG | + | chr7.2:40527448-40527467 | None:intergenic | 20.0% |
| !!! | TTTTTTCATTGAATCTTGTT+TGG | - | chr7.2:40528293-40528312 | MS.gene46157:CDS | 20.0% |
| ! | AATCTTGAAACCACAAAAAT+AGG | + | chr7.2:40528083-40528102 | None:intergenic | 25.0% |
| ! | TAATGTGAACAAATTACACT+TGG | - | chr7.2:40527194-40527213 | MS.gene46157:intron | 25.0% |
| ! | TTAATAATAGTGGTTGAAAC+TGG | - | chr7.2:40527716-40527735 | MS.gene46157:intron | 25.0% |
| ! | TTGTTGGAAATTGTAACATT+GGG | + | chr7.2:40526860-40526879 | None:intergenic | 25.0% |
| !! | TCTAGGAGAAATTTTTGTAT+AGG | - | chr7.2:40527258-40527277 | MS.gene46157:intron | 25.0% |
| !!! | GTAGTCATAGTTGTTTTAAA+TGG | - | chr7.2:40527901-40527920 | MS.gene46157:intron | 25.0% |
| AAATGAGAGTTTAGTGTTGT+TGG | + | chr7.2:40526876-40526895 | None:intergenic | 30.0% | |
| AACAACACTAAACTCTCATT+TGG | - | chr7.2:40526875-40526894 | MS.gene46157:CDS | 30.0% | |
| GTTGTTGGAAATTGTAACAT+TGG | + | chr7.2:40526861-40526880 | None:intergenic | 30.0% | |
| ! | TCATTGAATCTTGTTTGGAA+TGG | - | chr7.2:40528298-40528317 | MS.gene46157:CDS | 30.0% |
| !! | TTGTATAGGCATTTTTGGAT+TGG | - | chr7.2:40527272-40527291 | MS.gene46157:intron | 30.0% |
| !!! | GTCATAGTTGTTTTAAATGG+TGG | - | chr7.2:40527904-40527923 | MS.gene46157:intron | 30.0% |
| AACACTAAACTCTCATTTGG+AGG | - | chr7.2:40526878-40526897 | MS.gene46157:CDS | 35.0% | |
| AATGATTGTGTTCAAGGTCA+TGG | + | chr7.2:40526837-40526856 | None:intergenic | 35.0% | |
| ATAATAGTGGTTGAAACTGG+TGG | - | chr7.2:40527719-40527738 | MS.gene46157:intron | 35.0% | |
| ATTGGGAATGATTGTGTTCA+AGG | + | chr7.2:40526843-40526862 | None:intergenic | 35.0% | |
| TCTGTTATTGTTTCAGCAGT+TGG | - | chr7.2:40526953-40526972 | MS.gene46157:intron | 35.0% | |
| TCTTTACGAGAGAAAATGAC+AGG | + | chr7.2:40527052-40527071 | None:intergenic | 35.0% | |
| TGAAACTAAAAGCCGAAATC+AGG | + | chr7.2:40527587-40527606 | None:intergenic | 35.0% | |
| ! | TCGACTCTAAGTTGAGTTTA+AGG | - | chr7.2:40527611-40527630 | MS.gene46157:intron | 35.0% |
| !! | TGATAATGGGCAATGATGAT+TGG | - | chr7.2:40527809-40527828 | MS.gene46157:intron | 35.0% |
| ACAACGATCGGAGTGATAAT+GGG | - | chr7.2:40527796-40527815 | MS.gene46157:intron | 40.0% | |
| ACGGTCAGAATTATGATTGG+TGG | - | chr7.2:40527848-40527867 | MS.gene46157:intron | 40.0% | |
| AGTGGTGATCAGAGTATATG+TGG | - | chr7.2:40527931-40527950 | MS.gene46157:intron | 40.0% | |
| ATTCAAGGAGCAGTTCATGA+TGG | + | chr7.2:40528208-40528227 | None:intergenic | 40.0% | |
| ATTGTTTGTGCTCGTTGTGA+TGG | - | chr7.2:40528475-40528494 | MS.gene46157:CDS | 40.0% | |
| GCAACGGTCAGAATTATGAT+TGG | - | chr7.2:40527845-40527864 | MS.gene46157:intron | 40.0% | |
| GCTATTGGAATTGCTCTTGT+TGG | - | chr7.2:40527016-40527035 | MS.gene46157:intron | 40.0% | |
| GGCACTATTTATCTTGTTGC+TGG | - | chr7.2:40526992-40527011 | MS.gene46157:intron | 40.0% | |
| GTTGGAGATGTATCTTCTGA+TGG | - | chr7.2:40526971-40526990 | MS.gene46157:intron | 40.0% | |
| TACAACGATCGGAGTGATAA+TGG | - | chr7.2:40527795-40527814 | MS.gene46157:intron | 40.0% | |
| ! | TAATGGGCAATGATGATTGG+CGG | - | chr7.2:40527812-40527831 | MS.gene46157:intron | 40.0% |
| !! | GAGTATATGTGGTGTCATAG+TGG | - | chr7.2:40527942-40527961 | MS.gene46157:intron | 40.0% |
| !! | GTTTTAAATGGTGGTCACAG+TGG | - | chr7.2:40527913-40527932 | MS.gene46157:intron | 40.0% |
| !! | TATCTTGTTGCTGGTGCTAT+TGG | - | chr7.2:40527001-40527020 | MS.gene46157:intron | 40.0% |
| ATCAGATGCCCTGATTGTGA+TGG | - | chr7.2:40528385-40528404 | MS.gene46157:CDS | 45.0% | |
| CAAACAGTGGCTACAACGAT+CGG | - | chr7.2:40527784-40527803 | MS.gene46157:intron | 45.0% | |
| CTATGACTACCGATCAACTC+CGG | + | chr7.2:40527891-40527910 | None:intergenic | 45.0% | |
| GAGAGCAAATGCAGCAAGAA+AGG | - | chr7.2:40528441-40528460 | MS.gene46157:CDS | 45.0% | |
| GAGTGGTGAATAGCAAACAG+TGG | - | chr7.2:40527771-40527790 | MS.gene46157:intron | 45.0% | |
| TGGTGGTAAGAGGTGATCTA+AGG | - | chr7.2:40527972-40527991 | MS.gene46157:intron | 45.0% | |
| TGTGCTCGTTGTGATGGTTT+GGG | - | chr7.2:40528481-40528500 | MS.gene46157:CDS | 45.0% | |
| TTGTGCTCGTTGTGATGGTT+TGG | - | chr7.2:40528480-40528499 | MS.gene46157:CDS | 45.0% | |
| ! | AAGAGGTGATCTAAGGCAGT+TGG | - | chr7.2:40527979-40527998 | MS.gene46157:intron | 45.0% |
| ! | ATCTAAGGCAGTTGGAGTGA+TGG | - | chr7.2:40527987-40528006 | MS.gene46157:intron | 45.0% |
| ! | TGGTGGACAAGTGATTGCAA+TGG | - | chr7.2:40527865-40527884 | MS.gene46157:intron | 45.0% |
| !! | AGGAGGTGTTGGACAACTAT+CGG | - | chr7.2:40527749-40527768 | MS.gene46157:intron | 45.0% |
| !! | GGAGCAGGGTTTGTTAGAAA+GGG | - | chr7.2:40528406-40528425 | MS.gene46157:CDS | 45.0% |
| !! | TGGAGCAGGGTTTGTTAGAA+AGG | - | chr7.2:40528405-40528424 | MS.gene46157:CDS | 45.0% |
| !! | TGTGGTGTCATAGTGGTTGT+TGG | - | chr7.2:40527949-40527968 | MS.gene46157:intron | 45.0% |
| AACCCTGCTCCATCACAATC+AGG | + | chr7.2:40528397-40528416 | None:intergenic | 50.0% | |
| ACCCTGCTCCATCACAATCA+GGG | + | chr7.2:40528396-40528415 | None:intergenic | 50.0% | |
| GCAGTTGGAGTGATGGTTGA+CGG | - | chr7.2:40527994-40528013 | MS.gene46157:intron | 50.0% | |
| GGACAAGTGATTGCAATGGC+CGG | - | chr7.2:40527869-40527888 | MS.gene46157:intron | 50.0% | |
| GTGTTGGACAACTATCGGAG+TGG | - | chr7.2:40527754-40527773 | MS.gene46157:intron | 50.0% | |
| TGGTTGAAACTGGTGGCTGT+TGG | - | chr7.2:40527726-40527745 | MS.gene46157:intron | 50.0% | |
| TTGAAACTGGTGGCTGTTGG+AGG | - | chr7.2:40527729-40527748 | MS.gene46157:intron | 50.0% | |
| ! | AATGATGATTGGCGGACAGG+TGG | - | chr7.2:40527820-40527839 | MS.gene46157:intron | 50.0% |
| ! | GGTGTCATAGTGGTTGTTGG+TGG | - | chr7.2:40527952-40527971 | MS.gene46157:intron | 50.0% |
| ! | GTCATAGTGGTTGTTGGTGG+TGG | - | chr7.2:40527955-40527974 | MS.gene46157:intron | 50.0% |
| ! | TTGCAATGGCCGGAGTTGAT+CGG | - | chr7.2:40527879-40527898 | MS.gene46157:intron | 50.0% |
| !! | GCAGGGTTTGTTAGAAAGGG+TGG | - | chr7.2:40528409-40528428 | MS.gene46157:CDS | 50.0% |
| !! | TGGTTGTTGGTGGTGGTAAG+AGG | - | chr7.2:40527962-40527981 | MS.gene46157:intron | 50.0% |
| AAACTGGTGGCTGTTGGAGG+AGG | - | chr7.2:40527732-40527751 | MS.gene46157:intron | 55.0% | |
| GTTGACGGTGGTCGTAGTGT+TGG | - | chr7.2:40528009-40528028 | MS.gene46157:intron | 55.0% | |
| GTTGGAGTGATGGTTGACGG+TGG | - | chr7.2:40527997-40528016 | MS.gene46157:intron | 55.0% | |
| ! | GCCCTGATTGTGATGGAGCA+GGG | - | chr7.2:40528392-40528411 | MS.gene46157:CDS | 55.0% |
| ! | GGCAATGATGATTGGCGGAC+AGG | - | chr7.2:40527817-40527836 | MS.gene46157:intron | 55.0% |
| ! | TGCCCTGATTGTGATGGAGC+AGG | - | chr7.2:40528391-40528410 | MS.gene46157:CDS | 55.0% |
| !! | GTCGTAGTGTTGGTCGTTGG+TGG | - | chr7.2:40528019-40528038 | MS.gene46157:intron | 55.0% |
| !! | GTGGTCGTAGTGTTGGTCGT+TGG | - | chr7.2:40528016-40528035 | MS.gene46157:intron | 55.0% |
| ! | GTGGCTGTTGGAGGAGGTGT+TGG | - | chr7.2:40527738-40527757 | MS.gene46157:intron | 60.0% |
| ! | TGGCGGACAGGTGGTTGCAA+CGG | - | chr7.2:40527829-40527848 | MS.gene46157:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 40526827 | 40528528 | 40526827 | ID=MS.gene46157 |
| chr7.2 | mRNA | 40526827 | 40528528 | 40526827 | ID=MS.gene46157.t1;Parent=MS.gene46157 |
| chr7.2 | exon | 40528281 | 40528528 | 40528281 | ID=MS.gene46157.t1.exon1;Parent=MS.gene46157.t1 |
| chr7.2 | CDS | 40528281 | 40528528 | 40528281 | ID=cds.MS.gene46157.t1;Parent=MS.gene46157.t1 |
| chr7.2 | exon | 40526827 | 40526965 | 40526827 | ID=MS.gene46157.t1.exon2;Parent=MS.gene46157.t1 |
| chr7.2 | CDS | 40526827 | 40526965 | 40526827 | ID=cds.MS.gene46157.t1;Parent=MS.gene46157.t1 |
| Gene Sequence |
| Protein sequence |
