AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061049


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061049.t1 MTR_1g034260 96.094 128 4 1 1 128 1 127 5.80e-82 236
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061049.t1 AT2G26500 60.606 132 39 5 1 128 1 123 4.00e-41 133
MS.gene061049.t1 AT2G26500 60.606 132 39 5 1 128 1 123 4.00e-41 133
MS.gene061049.t1 AT2G26500 42.308 130 34 5 1 128 1 91 1.21e-18 75.1

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCCTCCTCCATTTGA+TGG 0.210124 1.4:+18610676 None:intergenic
TTTCTTAGTACTCCTTGATT+TGG 0.304270 1.4:+18610834 None:intergenic
GTTGTAGGAAGCTCAGTTAT+TGG 0.338271 1.4:-18610859 MS.gene061049:CDS
CCATCTACAATCACTGTTGT+AGG 0.380548 1.4:-18610874 MS.gene061049:CDS
CAGATTGCAGCTATCATGAA+TGG 0.408360 1.4:-18610607 MS.gene061049:CDS
ACTCTTGTTGGTGTTGCTGT+TGG 0.440680 1.4:-18610580 MS.gene061049:CDS
GGAGGAAGAAGAGGAGGTGC+AGG 0.460861 1.4:-18610664 MS.gene061049:CDS
CCTACAACAGTGATTGTAGA+TGG 0.469531 1.4:+18610874 None:intergenic
ATGAATGGACTTACTCTTGT+TGG 0.471407 1.4:-18610592 MS.gene061049:CDS
TCATTGAAATATCCATCAAA+TGG 0.499884 1.4:-18610688 MS.gene061049:CDS
ACAGGACTGAATTCATTCAA+TGG 0.502154 1.4:-18610790 MS.gene061049:CDS
TCTTCAACATGTAATGCTGC+AGG 0.547233 1.4:-18610640 MS.gene061049:CDS
CAGTTATTGGAACCAAATCA+AGG 0.561929 1.4:-18610846 MS.gene061049:CDS
AAATCAAGGAGTACTAAGAA+AGG 0.563363 1.4:-18610832 MS.gene061049:CDS
TCAAATGGAGGAGGAAGAAG+AGG 0.566468 1.4:-18610673 MS.gene061049:CDS
AAATATCCATCAAATGGAGG+AGG 0.584326 1.4:-18610682 MS.gene061049:CDS
AGCAATGTGAAGTTTATCAC+AGG 0.595651 1.4:-18610808 MS.gene061049:CDS
AATGGAGGAGGAAGAAGAGG+AGG 0.611502 1.4:-18610670 MS.gene061049:CDS
TTGAAATATCCATCAAATGG+AGG 0.614695 1.4:-18610685 MS.gene061049:CDS
AACAGCACTGTGACATCACT+AGG 0.621698 1.4:-18610754 MS.gene061049:CDS
TGTAGATGGAGTTGTTGCTG+AGG 0.669064 1.4:+18610888 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCATTGAAATATCCATCAAA+TGG - chr1.4:18610744-18610763 MS.gene061049:CDS 25.0%
TTGAAATATCCATCAAATGG+AGG - chr1.4:18610747-18610766 MS.gene061049:CDS 30.0%
TTTCTTAGTACTCCTTGATT+TGG + chr1.4:18610601-18610620 None:intergenic 30.0%
!! AAATCAAGGAGTACTAAGAA+AGG - chr1.4:18610600-18610619 MS.gene061049:CDS 30.0%
AAATATCCATCAAATGGAGG+AGG - chr1.4:18610750-18610769 MS.gene061049:CDS 35.0%
ACAGGACTGAATTCATTCAA+TGG - chr1.4:18610642-18610661 MS.gene061049:CDS 35.0%
AGCAATGTGAAGTTTATCAC+AGG - chr1.4:18610624-18610643 MS.gene061049:CDS 35.0%
CAGTTATTGGAACCAAATCA+AGG - chr1.4:18610586-18610605 MS.gene061049:CDS 35.0%
! ATGAATGGACTTACTCTTGT+TGG - chr1.4:18610840-18610859 MS.gene061049:CDS 35.0%
CAGATTGCAGCTATCATGAA+TGG - chr1.4:18610825-18610844 MS.gene061049:CDS 40.0%
CCATCTACAATCACTGTTGT+AGG - chr1.4:18610558-18610577 MS.gene061049:CDS 40.0%
CCTACAACAGTGATTGTAGA+TGG + chr1.4:18610561-18610580 None:intergenic 40.0%
GTTGTAGGAAGCTCAGTTAT+TGG - chr1.4:18610573-18610592 MS.gene061049:CDS 40.0%
TCTTCAACATGTAATGCTGC+AGG - chr1.4:18610792-18610811 MS.gene061049:CDS 40.0%
AACAGCACTGTGACATCACT+AGG - chr1.4:18610678-18610697 MS.gene061049:CDS 45.0%
CTTCTTCCTCCTCCATTTGA+TGG + chr1.4:18610759-18610778 None:intergenic 45.0%
TCAAATGGAGGAGGAAGAAG+AGG - chr1.4:18610759-18610778 MS.gene061049:CDS 45.0%
TGTAGATGGAGTTGTTGCTG+AGG + chr1.4:18610547-18610566 None:intergenic 45.0%
!! ACTCTTGTTGGTGTTGCTGT+TGG - chr1.4:18610852-18610871 MS.gene061049:CDS 45.0%
AATGGAGGAGGAAGAAGAGG+AGG - chr1.4:18610762-18610781 MS.gene061049:CDS 50.0%
! GGAGGAAGAAGAGGAGGTGC+AGG - chr1.4:18610768-18610787 MS.gene061049:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 18610534 18610920 18610534 ID=MS.gene061049
chr1.4 mRNA 18610534 18610920 18610534 ID=MS.gene061049.t1;Parent=MS.gene061049
chr1.4 exon 18610534 18610920 18610534 ID=MS.gene061049.t1.exon1;Parent=MS.gene061049.t1
chr1.4 CDS 18610534 18610920 18610534 ID=cds.MS.gene061049.t1;Parent=MS.gene061049.t1
Gene Sequence

>MS.gene061049

ATGGCTACAGCCTCAGCAACAACTCCATCTACAATCACTGTTGTAGGAAGCTCAGTTATTGGAACCAAATCAAGGAGTACTAAGAAAGGTAGCAATGTGAAGTTTATCACAGGACTGAATTCATTCAATGGATTGAAAGCACAGAACAGCACTGTGACATCACTAGGAGTTTCTATGAGCACTGAACAAGCTTTTGCAAAAGTGATGAGTTCATTGAAATATCCATCAAATGGAGGAGGAAGAAGAGGAGGTGCAGGTTCTTCAACATGTAATGCTGCAGGTGAGATTTTTCAGATTGCAGCTATCATGAATGGACTTACTCTTGTTGGTGTTGCTGTTGGATTTGTTCTTCTTCGAATTGAAGCTGCTGTTGAAGAAGCTGAGTAA

Protein sequence

>MS.gene061049.t1

MATASATTPSTITVVGSSVIGTKSRSTKKGSNVKFITGLNSFNGLKAQNSTVTSLGVSMSTEQAFAKVMSSLKYPSNGGGRRGGAGSSTCNAAGEIFQIAAIMNGLTLVGVAVGFVLLRIEAAVEEAE