Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46626.t1 | PNX68612.1 | 93.8 | 32 | 2 | 0 | 1 | 32 | 1 | 32 | 9.00E-10 | 72.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46626.t1 | A0A2K3KQQ2 | 93.8 | 32 | 2 | 0 | 1 | 32 | 1 | 32 | 6.5e-10 | 72.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene46626 | 0.808593 | 2.87E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46626.t1 | MTR_3g032740 | 97.015 | 67 | 2 | 0 | 1 | 67 | 1 | 67 | 7.87e-44 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46626.t1 | AT3G57450 | 53.488 | 43 | 17 | 1 | 1 | 40 | 1 | 43 | 3.94e-11 | 53.9 |
Find 22 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTAAATCTTCTGGTGGTTT+TGG | 0.295854 | 3.3:-24059768 | MS.gene46626:CDS |
| GGCGGCGGTGGTAAATCTTC+TGG | 0.387547 | 3.3:-24059777 | MS.gene46626:CDS |
| TAAAACTTCCTTCCCGTTTG+AGG | 0.400930 | 3.3:+24059846 | None:intergenic |
| TCTGTCAACGGTTGCGGCGG+CGG | 0.409164 | 3.3:-24059792 | MS.gene46626:CDS |
| GTCAACGGTTGCGGCGGCGG+TGG | 0.459160 | 3.3:-24059789 | MS.gene46626:CDS |
| GGAGCGTCTGTCAACGGTTG+CGG | 0.469497 | 3.3:-24059798 | MS.gene46626:CDS |
| TTATTCCAACTGTCCTCAAA+CGG | 0.470576 | 3.3:-24059859 | MS.gene46626:CDS |
| CCTTCCCGTTTGAGGACAGT+TGG | 0.500910 | 3.3:+24059854 | None:intergenic |
| TATTCCAACTGTCCTCAAAC+GGG | 0.503460 | 3.3:-24059858 | MS.gene46626:CDS |
| TTCAGAGATGGGAAAGTGGG+TGG | 0.529663 | 3.3:-24059919 | None:intergenic |
| TGGGTGGAGATTGTTGATCA+AGG | 0.538256 | 3.3:-24059903 | MS.gene46626:CDS |
| GACGCTCCTCCTTCAGAATG+AGG | 0.556617 | 3.3:+24059813 | None:intergenic |
| CCTCCTTCAGAATGAGGCGG+AGG | 0.559653 | 3.3:+24059819 | None:intergenic |
| AGATTGTTGATCAAGGAATG+AGG | 0.570438 | 3.3:-24059896 | MS.gene46626:CDS |
| CATCCTCCGCCTCATTCTGA+AGG | 0.570790 | 3.3:-24059822 | MS.gene46626:CDS |
| GAAGGAGGAGCGTCTGTCAA+CGG | 0.589595 | 3.3:-24059804 | MS.gene46626:CDS |
| GCGTCTGTCAACGGTTGCGG+CGG | 0.597066 | 3.3:-24059795 | MS.gene46626:CDS |
| GGCGGTGGTAAATCTTCTGG+TGG | 0.599204 | 3.3:-24059774 | MS.gene46626:CDS |
| CCTCCGCCTCATTCTGAAGG+AGG | 0.601695 | 3.3:-24059819 | MS.gene46626:CDS |
| AAGGAATGAGGATTGTTGCA+AGG | 0.641797 | 3.3:-24059884 | MS.gene46626:CDS |
| CCAACTGTCCTCAAACGGGA+AGG | 0.652789 | 3.3:-24059854 | MS.gene46626:CDS |
| GCTCCTCCTTCAGAATGAGG+CGG | 0.667236 | 3.3:+24059816 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATTGTTGATCAAGGAATG+AGG | - | chr3.3:24059747-24059766 | MS.gene46626:CDS | 35.0% | |
| TTATTCCAACTGTCCTCAAA+CGG | - | chr3.3:24059784-24059803 | MS.gene46626:CDS | 35.0% | |
| !!! | TGGTTTTGGTGGAAATAGTT+TGG | - | chr3.3:24059889-24059908 | MS.gene46626:CDS | 35.0% |
| AAGGAATGAGGATTGTTGCA+AGG | - | chr3.3:24059759-24059778 | MS.gene46626:CDS | 40.0% | |
| TAAAACTTCCTTCCCGTTTG+AGG | + | chr3.3:24059800-24059819 | None:intergenic | 40.0% | |
| TATTCCAACTGTCCTCAAAC+GGG | - | chr3.3:24059785-24059804 | MS.gene46626:CDS | 40.0% | |
| ! | GGTAAATCTTCTGGTGGTTT+TGG | - | chr3.3:24059875-24059894 | MS.gene46626:CDS | 40.0% |
| !!! | AAATCTTCTGGTGGTTTTGG+TGG | - | chr3.3:24059878-24059897 | MS.gene46626:CDS | 40.0% |
| TGGGTGGAGATTGTTGATCA+AGG | - | chr3.3:24059740-24059759 | MS.gene46626:CDS | 45.0% | |
| CATCCTCCGCCTCATTCTGA+AGG | - | chr3.3:24059821-24059840 | MS.gene46626:CDS | 55.0% | |
| CCAACTGTCCTCAAACGGGA+AGG | - | chr3.3:24059789-24059808 | MS.gene46626:CDS | 55.0% | |
| CCTTCCCGTTTGAGGACAGT+TGG | + | chr3.3:24059792-24059811 | None:intergenic | 55.0% | |
| GACGCTCCTCCTTCAGAATG+AGG | + | chr3.3:24059833-24059852 | None:intergenic | 55.0% | |
| GCTCCTCCTTCAGAATGAGG+CGG | + | chr3.3:24059830-24059849 | None:intergenic | 55.0% | |
| GGCGGTGGTAAATCTTCTGG+TGG | - | chr3.3:24059869-24059888 | MS.gene46626:CDS | 55.0% | |
| !! | GAAGGAGGAGCGTCTGTCAA+CGG | - | chr3.3:24059839-24059858 | MS.gene46626:CDS | 55.0% |
| CCTCCTTCAGAATGAGGCGG+AGG | + | chr3.3:24059827-24059846 | None:intergenic | 60.0% | |
| GGCGGCGGTGGTAAATCTTC+TGG | - | chr3.3:24059866-24059885 | MS.gene46626:CDS | 60.0% | |
| ! | CCTCCGCCTCATTCTGAAGG+AGG | - | chr3.3:24059824-24059843 | MS.gene46626:CDS | 60.0% |
| ! | GGAGCGTCTGTCAACGGTTG+CGG | - | chr3.3:24059845-24059864 | MS.gene46626:CDS | 60.0% |
| GCGTCTGTCAACGGTTGCGG+CGG | - | chr3.3:24059848-24059867 | MS.gene46626:CDS | 65.0% | |
| TCTGTCAACGGTTGCGGCGG+CGG | - | chr3.3:24059851-24059870 | MS.gene46626:CDS | 65.0% | |
| GTCAACGGTTGCGGCGGCGG+TGG | - | chr3.3:24059854-24059873 | MS.gene46626:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 24059731 | 24059934 | 24059731 | ID=MS.gene46626 |
| chr3.3 | mRNA | 24059731 | 24059934 | 24059731 | ID=MS.gene46626.t1;Parent=MS.gene46626 |
| chr3.3 | exon | 24059731 | 24059934 | 24059731 | ID=MS.gene46626.t1.exon1;Parent=MS.gene46626.t1 |
| chr3.3 | CDS | 24059731 | 24059934 | 24059731 | ID=cds.MS.gene46626.t1;Parent=MS.gene46626.t1 |
| Gene Sequence |
| Protein sequence |
