AlfalfaGEDB | MS.gene46922

M. sativa cultivar XinJiangDaYe / MS.gene46922

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46922.t1	RHN63858.1	75	88	9	1	1	88	1	75	6.20E-27	129.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46922.t1	A0A396IGD8	75.0	88	9	1	1	88	1	75	4.5e-27	129.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056752	MS.gene46922	0.811892	5.58E-51	-1.69E-46
MS.gene056975	MS.gene46922	0.803125	4.05E-49	-1.69E-46
MS.gene057452	MS.gene46922	-0.819688	1.02E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 12 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTAACTTGCACGAAGCTTCT+TGG	0.292272	8.4:+12278182	None:intergenic
AGCTCTGAACTAGTCCCTTT+CGG	0.363061	8.4:+12278091	None:intergenic
TTCGTGCAAGTTACAACAAA+AGG	0.399654	8.4:-12278173	MS.gene46922:CDS
TGACACCATGCGTATTGTTA+AGG	0.423021	8.4:-12278010	None:intergenic
CGGTTCCTGTACATCAAAAG+AGG	0.488001	8.4:+12278111	None:intergenic
TCGTGCAAGTTACAACAAAA+GGG	0.561864	8.4:-12278172	MS.gene46922:CDS
TTGATGTACAGGAACCGAAA+GGG	0.576200	8.4:-12278105	MS.gene46922:CDS
TTTGATGTACAGGAACCGAA+AGG	0.590508	8.4:-12278106	MS.gene46922:CDS
GTGTCACAATCAAACACATA+GGG	0.599883	8.4:+12278027	None:intergenic
GGTGTCACAATCAAACACAT+AGG	0.607030	8.4:+12278026	None:intergenic
ACTTGCCTTAACAATACGCA+TGG	0.647656	8.4:+12278005	None:intergenic
ACATCAAAAGAGGAGTCTGT+CGG	0.656940	8.4:+12278121	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATACTATATATAAAAAGAA+TGG	-	chr8.4:12278548-12278567	MS.gene46922:intron	10.0%
!!	AGGTAAAAATAGAATGAAAT+GGG	+	chr8.4:12278281-12278300	None:intergenic	20.0%
!!	TAGGTAAAAATAGAATGAAA+TGG	+	chr8.4:12278282-12278301	None:intergenic	20.0%
!!	TTGATCAATTAAGAAATGAA+GGG	+	chr8.4:12278394-12278413	None:intergenic	20.0%
!!	TTGTTAAGAAACAAAAAGTA+GGG	-	chr8.4:12278030-12278049	MS.gene46922:CDS	20.0%
!!	TTTGATCAATTAAGAAATGA+AGG	+	chr8.4:12278395-12278414	None:intergenic	20.0%
!!!	CTTTGCTGTTAAATTAATTT+GGG	-	chr8.4:12278115-12278134	MS.gene46922:CDS	20.0%
!!!	TCTTTGCTGTTAAATTAATT+TGG	-	chr8.4:12278114-12278133	MS.gene46922:CDS	20.0%
!	GTTGTTAAGAAACAAAAAGT+AGG	-	chr8.4:12278029-12278048	MS.gene46922:CDS	25.0%
!	TGTTAAGAAACAAAAAGTAG+GGG	-	chr8.4:12278031-12278050	MS.gene46922:CDS	25.0%
!!	AGTTTTACTCAATGTTGTAA+TGG	-	chr8.4:12278078-12278097	MS.gene46922:CDS	25.0%
	GAAAAATAGAAGACATGCAT+AGG	+	chr8.4:12278301-12278320	None:intergenic	30.0%
	GTTAAGAAACAAAAAGTAGG+GGG	-	chr8.4:12278032-12278051	MS.gene46922:CDS	30.0%
!!!	AGGGTGAGGTTATTTTAATA+TGG	+	chr8.4:12278375-12278394	None:intergenic	30.0%
	CAATTAAGAAATGAAGGGTG+AGG	+	chr8.4:12278389-12278408	None:intergenic	35.0%
	GTGTCACAATCAAACACATA+GGG	+	chr8.4:12278590-12278609	None:intergenic	35.0%
	TCGTGCAAGTTACAACAAAA+GGG	-	chr8.4:12278442-12278461	MS.gene46922:intron	35.0%
	TTCGTGCAAGTTACAACAAA+AGG	-	chr8.4:12278441-12278460	MS.gene46922:intron	35.0%
!!	GCATGTCTTCTATTTTTCTG+TGG	-	chr8.4:12278303-12278322	MS.gene46922:intron	35.0%
	ACATCAAAAGAGGAGTCTGT+CGG	+	chr8.4:12278496-12278515	None:intergenic	40.0%
	TTGATGTACAGGAACCGAAA+GGG	-	chr8.4:12278509-12278528	MS.gene46922:intron	40.0%
	TTTGATGTACAGGAACCGAA+AGG	-	chr8.4:12278508-12278527	MS.gene46922:intron	40.0%
!	AGACTCCTCTTTTGATGTAC+AGG	-	chr8.4:12278498-12278517	MS.gene46922:intron	40.0%
!	GGTGTCACAATCAAACACAT+AGG	+	chr8.4:12278591-12278610	None:intergenic	40.0%
	CGGTTCCTGTACATCAAAAG+AGG	+	chr8.4:12278506-12278525	None:intergenic	45.0%
!	GTAACTTGCACGAAGCTTCT+TGG	+	chr8.4:12278435-12278454	None:intergenic	45.0%
!!	AGCTCTGAACTAGTCCCTTT+CGG	+	chr8.4:12278526-12278545	None:intergenic	45.0%
	TATCTTGCCATTGTTGCGCC+AGG	-	chr8.4:12278241-12278260	MS.gene46922:intron	50.0%
	TGGGCTGAATTTCCCAAGCC+TGG	+	chr8.4:12278262-12278281	None:intergenic	55.0%
!	GTAGGGGGCACAAAGCGAAA+AGG	-	chr8.4:12278047-12278066	MS.gene46922:CDS	55.0%
	GCCATTGTTGCGCCAGGCTT+GGG	-	chr8.4:12278247-12278266	MS.gene46922:intron	60.0%
	TCCCAAGCCTGGCGCAACAA+TGG	+	chr8.4:12278251-12278270	None:intergenic	60.0%
	TGCCATTGTTGCGCCAGGCT+TGG	-	chr8.4:12278246-12278265	MS.gene46922:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	12278011	12278625	12278011	ID=MS.gene46922
chr8.4	mRNA	12278011	12278625	12278011	ID=MS.gene46922.t1;Parent=MS.gene46922
chr8.4	exon	12278562	12278625	12278562	ID=MS.gene46922.t1.exon1;Parent=MS.gene46922.t1
chr8.4	CDS	12278562	12278625	12278562	ID=cds.MS.gene46922.t1;Parent=MS.gene46922.t1
chr8.4	exon	12278011	12278210	12278011	ID=MS.gene46922.t1.exon2;Parent=MS.gene46922.t1
chr8.4	CDS	12278011	12278210	12278011	ID=cds.MS.gene46922.t1;Parent=MS.gene46922.t1

Gene Sequence

>MS.gene46922

ATGACAAGCAAGAATGATGTTGTTAAGAAACAAAAAGTAGGGGGCACAAAGCGAAAAGGAGAAAGTAAGTTTTACTCAATGTTGTAATGGTATATTGATATGTTCTTTGCTGTTAAATTAATTTGGGTTAACATTTTGACTTTAAAGCTATGCGAAGATACAATTATTTTTGCTTTCTTGTTTTTATGAAAATGCAAGTTCTTGATTGAGTGTTAATATGCACATGTGTTTATCTTGCCATTGTTGCGCCAGGCTTGGGAAATTCAGCCCATTTCATTCTATTTTTACCTATGCATGTCTTCTATTTTTCTGTGGCTGTTGTGCGTTGTCTTTCTTCTTCTTTTCTTTTCAACAGTTTCAGCCATATTAAAATAACCTCACCCTTCATTTCTTAATTGATCAAAACATTAAACAGAATTTGCCAAGAAGCTTCGTGCAAGTTACAACAAAAGGGTAGCTGCTAAGAAGCAAGAATTCATGAACCGACAGACTCCTCTTTTGATGTACAGGAACCGAAAGGGACTAGTTCAGAGCTATAATACTATATATAAAAAGAATGGCGATTGTGTTACTACTCCCTATGTGTTTGATTGTGACACCATGCGTATTGTTAAG

Protein sequence

>MS.gene46922.t1

MTSKNDVVKKQKVGGTKRKGEKFAKKLRASYNKRVAAKKQEFMNRQTPLLMYRNRKGLVQSYNTIYKKNGDCVTTPYVFDCDTMRIVK