AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47020.t1 MTR_4g113270 98.266 346 5 1 1 346 1 345 0.0 676
MS.gene47020.t1 MTR_4g113270 98.235 340 5 1 1 340 1 339 0.0 665
MS.gene47020.t1 MTR_1g100860 47.788 339 158 4 15 340 41 373 5.73e-98 295
MS.gene47020.t1 MTR_4g018770 47.198 339 160 4 15 340 35 367 8.72e-98 294
MS.gene47020.t1 MTR_1g100860 46.739 276 130 4 77 340 5 275 4.57e-76 235
MS.gene47020.t1 MTR_4g018770 46.570 277 129 4 77 340 5 275 6.75e-76 235
MS.gene47020.t1 MTR_3g463360 24.242 297 206 6 19 304 108 396 9.01e-13 69.3
MS.gene47020.t1 MTR_3g109070 27.692 260 164 7 18 267 20 265 2.14e-12 67.4
MS.gene47020.t1 MTR_3g109070 27.692 260 164 7 18 267 20 265 3.48e-12 66.2
MS.gene47020.t1 MTR_3g096310 23.102 303 218 5 18 311 45 341 6.48e-12 66.2
MS.gene47020.t1 MTR_3g109070 28.218 202 126 5 18 210 20 211 2.80e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47020.t1 AT5G19980 71.304 345 92 2 2 346 3 340 6.17e-169 474
MS.gene47020.t1 AT1G76340 48.615 325 161 3 15 337 37 357 9.10e-101 302
MS.gene47020.t1 AT4G31600 26.848 257 170 5 18 267 19 264 3.66e-13 69.7
MS.gene47020.t1 AT2G13650 22.896 297 212 5 18 304 38 327 8.51e-12 65.9
MS.gene47020.t1 AT2G13650 22.896 297 212 5 18 304 38 327 8.51e-12 65.9
MS.gene47020.t1 AT4G31600 27.136 199 132 3 18 210 19 210 1.26e-11 65.1

Find 65 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGGTTGTTATTCTTGC+TGG 0.233454 8.4:+15121696 MS.gene47020:CDS
GCTACCGCGTTTACCGTTAC+TGG 0.269983 8.4:+15122062 MS.gene47020:CDS
GGTTTGTTGATTAGTTTCTT+TGG 0.270396 8.4:+15122014 MS.gene47020:CDS
GAAACCCTAATTTACCTAAA+AGG 0.287655 8.4:-15121464 None:intergenic
TTACACTCACGGCTTATTCT+TGG 0.301434 8.4:+15121751 MS.gene47020:CDS
TGAGTCTTGGGTTGAATACT+TGG 0.340183 8.4:+15121832 MS.gene47020:CDS
AATAACAACCAAGGACAAAA+AGG 0.342091 8.4:-15121688 None:intergenic
TACACTCACGGCTTATTCTT+GGG 0.343245 8.4:+15121752 MS.gene47020:CDS
GGTTATGTTGCTACTGATTC+CGG 0.348332 8.4:+15121726 MS.gene47020:CDS
CTACCGCGTTTACCGTTACT+GGG 0.359242 8.4:+15122063 MS.gene47020:CDS
TGGCAATTAATGTGACGATT+TGG 0.360897 8.4:+15122108 MS.gene47020:CDS
GTTTCGTTGTCTTGTGTGTT+TGG 0.368137 8.4:+15121993 MS.gene47020:CDS
GATGGTTTATATTAAGCATA+TGG 0.374607 8.4:+15121806 MS.gene47020:CDS
GCATGCTAGTCCAGCTGGTT+TGG 0.378044 8.4:+15122136 MS.gene47020:CDS
CAGCAATCGGTGACTGGAAA+TGG 0.381275 8.4:+15122197 MS.gene47020:CDS
AAGCATATGGTTATGAGTCT+TGG 0.383947 8.4:+15121819 MS.gene47020:CDS
AGCATATGGTTATGAGTCTT+GGG 0.397212 8.4:+15121820 MS.gene47020:CDS
GGCAATTAATGTGACGATTT+GGG 0.402674 8.4:+15122109 MS.gene47020:CDS
GTTATTCTTGCTGGTGCTGT+TGG 0.419854 8.4:+15121705 MS.gene47020:CDS
GACTGGAAATGGTTCGCAAC+AGG 0.428349 8.4:+15122208 MS.gene47020:CDS
GATGTTGAAAGTAGTCTTGT+TGG 0.433983 8.4:+15122257 MS.gene47020:CDS
GCCACAAGTGGCTTAGTAAT+CGG 0.437849 8.4:+15121327 MS.gene47020:CDS
GGGAAGAATTTCTTAGCGAT+TGG 0.443284 8.4:-15121510 None:intergenic
AAGAAGAAAACGAGTGCTGC+AGG 0.450306 8.4:-15121531 None:intergenic
GAGTCTTGGGTTGAATACTT+GGG 0.451840 8.4:+15121833 MS.gene47020:CDS
CTTTATCAGCAATCGGTGAC+TGG 0.470901 8.4:+15122191 MS.gene47020:CDS
GGGTGTTCTTTATCAGCAAT+CGG 0.471127 8.4:+15122184 MS.gene47020:CDS
TGGTTCGCAACAGGCGGTTG+TGG 0.475038 8.4:+15122217 MS.gene47020:CDS
GTTAGCGTGACGAAGGAGAT+TGG 0.477146 8.4:-15121571 None:intergenic
TGAAGAAGTGAGATATTGTA+AGG 0.495489 8.4:-15121430 None:intergenic
ATAAGCCGTGAGTGTAAAAC+CGG 0.495535 8.4:-15121745 None:intergenic
ACTGCCTTGAGATCGAGTAC+TGG 0.496102 8.4:+15121939 MS.gene47020:CDS
TGTAAGGCTGTTAAAAGACC+TGG 0.499578 8.4:-15121414 None:intergenic
CAATATCTCACTTCTTCACT+CGG 0.505402 8.4:+15121435 MS.gene47020:CDS
CTGCCTTGAGATCGAGTACT+GGG 0.507997 8.4:+15121940 MS.gene47020:CDS
AGTCTTGGGTTGAATACTTG+GGG 0.509311 8.4:+15121834 MS.gene47020:CDS
GATAAGCATGCTAGTCCAGC+TGG 0.512847 8.4:+15122131 MS.gene47020:CDS
TGAAGATGAGAGTGAAGGAA+AGG 0.515215 8.4:+15122292 MS.gene47020:CDS
CTCGCTGATACTGCGTTTCG+CGG 0.516331 8.4:+15121642 MS.gene47020:CDS
ATAAACCATCTCAGTAGTAA+TGG 0.529935 8.4:-15121793 None:intergenic
GAAGATGAGAGTGAAGGAAA+GGG 0.531374 8.4:+15122293 MS.gene47020:CDS
AGCAAACAAACCAAACCAGC+TGG 0.537823 8.4:-15122146 None:intergenic
TTAGCGATTGGAATAGTGAA+AGG 0.542673 8.4:-15121498 None:intergenic
GGTTACCATTACTACTGAGA+TGG 0.551400 8.4:+15121788 MS.gene47020:CDS
CAGCGAGAGCAACAAGAAGA+GGG 0.556974 8.4:-15121626 None:intergenic
TCAGCGAGAGCAACAAGAAG+AGG 0.569697 8.4:-15121627 None:intergenic
CAACAACACTACGCCACAAG+TGG 0.587960 8.4:+15121315 MS.gene47020:CDS
GAAAGTAGTCTTGTTGGTGA+TGG 0.589182 8.4:+15122263 MS.gene47020:CDS
ACCAGCAAGAATAACAACCA+AGG 0.595444 8.4:-15121697 None:intergenic
AAGGTAAAATTAGACGGAGA+GGG 0.604146 8.4:-15121669 None:intergenic
CTTATTCACAACCCCAGTAA+CGG 0.607538 8.4:-15122075 None:intergenic
GTAAGGCTGTTAAAAGACCT+GGG 0.614797 8.4:-15121413 None:intergenic
AAAGGTAAAATTAGACGGAG+AGG 0.624222 8.4:-15121670 None:intergenic
GTGACGAAGGAGATTGGTGT+TGG 0.632002 8.4:-15121565 None:intergenic
ACTCCCAGTACTCGATCTCA+AGG 0.635928 8.4:-15121943 None:intergenic
ACCGATTACTAAGCCACTTG+TGG 0.637733 8.4:-15121328 None:intergenic
TATCAACGTTAGCGTGACGA+AGG 0.639770 8.4:-15121578 None:intergenic
TGGAAATGGTTCGCAACAGG+CGG 0.646327 8.4:+15122211 MS.gene47020:CDS
ATTACTCAATTCAACTACCC+AGG 0.649152 8.4:+15121396 MS.gene47020:CDS
AGAAGAAAACGAGTGCTGCA+GGG 0.656660 8.4:-15121530 None:intergenic
AGCGAGAGCAACAAGAAGAG+GGG 0.662395 8.4:-15121625 None:intergenic
TGTGAATAAGTTTCTGACTG+TGG 0.688581 8.4:+15122088 MS.gene47020:CDS
GAATCTGAAGATGAGAGTGA+AGG 0.699145 8.4:+15122287 MS.gene47020:CDS
TACCGCGTTTACCGTTACTG+GGG 0.705827 8.4:+15122064 MS.gene47020:CDS
AACCCCAGTAACGGTAAACG+CGG 0.739549 8.4:-15122066 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTACCTTTTAGGTAAATTA+GGG + chr8.4:15121460-15121479 MS.gene47020:CDS 20.0%
! GACAAAAAGGTAAAATTAGA+CGG - chr8.4:15121678-15121697 None:intergenic 25.0%
! GATGGTTTATATTAAGCATA+TGG + chr8.4:15121806-15121825 MS.gene47020:CDS 25.0%
!! TAATTTTACCTTTTTGTCCT+TGG + chr8.4:15121680-15121699 MS.gene47020:CDS 25.0%
!!! GTTTACCTTTTAGGTAAATT+AGG + chr8.4:15121459-15121478 MS.gene47020:CDS 25.0%
!!! GTTTGCTTTTTACGATTATT+GGG + chr8.4:15122162-15122181 MS.gene47020:CDS 25.0%
!!! TGTTTGCTTTTTACGATTAT+TGG + chr8.4:15122161-15122180 MS.gene47020:CDS 25.0%
!!! TTTGCTTTTTACGATTATTG+GGG + chr8.4:15122163-15122182 MS.gene47020:CDS 25.0%
AATAACAACCAAGGACAAAA+AGG - chr8.4:15121691-15121710 None:intergenic 30.0%
ATAAACCATCTCAGTAGTAA+TGG - chr8.4:15121796-15121815 None:intergenic 30.0%
GAAACCCTAATTTACCTAAA+AGG - chr8.4:15121467-15121486 None:intergenic 30.0%
TGAAGAAGTGAGATATTGTA+AGG - chr8.4:15121433-15121452 None:intergenic 30.0%
!! GGTTTGTTGATTAGTTTCTT+TGG + chr8.4:15122014-15122033 MS.gene47020:CDS 30.0%
!!! TTGCTTTTTACGATTATTGG+GGG + chr8.4:15122164-15122183 MS.gene47020:CDS 30.0%
AAAGGTAAAATTAGACGGAG+AGG - chr8.4:15121673-15121692 None:intergenic 35.0%
AAGCATATGGTTATGAGTCT+TGG + chr8.4:15121819-15121838 MS.gene47020:CDS 35.0%
AAGGTAAAATTAGACGGAGA+GGG - chr8.4:15121672-15121691 None:intergenic 35.0%
AGCATATGGTTATGAGTCTT+GGG + chr8.4:15121820-15121839 MS.gene47020:CDS 35.0%
ATTACTCAATTCAACTACCC+AGG + chr8.4:15121396-15121415 MS.gene47020:CDS 35.0%
CAATATCTCACTTCTTCACT+CGG + chr8.4:15121435-15121454 MS.gene47020:CDS 35.0%
CCAGTCAAAAACCAAAAAAC+AGG - chr8.4:15121900-15121919 None:intergenic 35.0%
GGCAATTAATGTGACGATTT+GGG + chr8.4:15122109-15122128 MS.gene47020:CDS 35.0%
TGGCAATTAATGTGACGATT+TGG + chr8.4:15122108-15122127 MS.gene47020:CDS 35.0%
TGTGAATAAGTTTCTGACTG+TGG + chr8.4:15122088-15122107 MS.gene47020:CDS 35.0%
TTAGCGATTGGAATAGTGAA+AGG - chr8.4:15121501-15121520 None:intergenic 35.0%
! TTCTTGGGCTTTTGCTTATT+TGG + chr8.4:15121767-15121786 MS.gene47020:CDS 35.0%
! TTTGACTGGTGAGAATTTTG+AGG + chr8.4:15121911-15121930 MS.gene47020:CDS 35.0%
!! GATGTTGAAAGTAGTCTTGT+TGG + chr8.4:15122257-15122276 MS.gene47020:CDS 35.0%
!!! CCTGTTTTTTGGTTTTTGAC+TGG + chr8.4:15121897-15121916 MS.gene47020:CDS 35.0%
!!! TGATGATTGCTCCTGTTTTT+TGG + chr8.4:15121886-15121905 MS.gene47020:CDS 35.0%
ACCAGCAAGAATAACAACCA+AGG - chr8.4:15121700-15121719 None:intergenic 40.0%
ATAAGCCGTGAGTGTAAAAC+CGG - chr8.4:15121748-15121767 None:intergenic 40.0%
CTTATTCACAACCCCAGTAA+CGG - chr8.4:15122078-15122097 None:intergenic 40.0%
GAAGATGAGAGTGAAGGAAA+GGG + chr8.4:15122293-15122312 MS.gene47020:CDS 40.0%
GAATCTGAAGATGAGAGTGA+AGG + chr8.4:15122287-15122306 MS.gene47020:CDS 40.0%
GGGAAGAATTTCTTAGCGAT+TGG - chr8.4:15121513-15121532 None:intergenic 40.0%
GGGTGTTCTTTATCAGCAAT+CGG + chr8.4:15122184-15122203 MS.gene47020:CDS 40.0%
GGTTACCATTACTACTGAGA+TGG + chr8.4:15121788-15121807 MS.gene47020:CDS 40.0%
GTAAGGCTGTTAAAAGACCT+GGG - chr8.4:15121416-15121435 None:intergenic 40.0%
GTTTCGTTGTCTTGTGTGTT+TGG + chr8.4:15121993-15122012 MS.gene47020:CDS 40.0%
TACACTCACGGCTTATTCTT+GGG + chr8.4:15121752-15121771 MS.gene47020:CDS 40.0%
TGAAGATGAGAGTGAAGGAA+AGG + chr8.4:15122292-15122311 MS.gene47020:CDS 40.0%
TGTAAGGCTGTTAAAAGACC+TGG - chr8.4:15121417-15121436 None:intergenic 40.0%
TTACACTCACGGCTTATTCT+TGG + chr8.4:15121751-15121770 MS.gene47020:CDS 40.0%
! GTTCGATGTGAATGCGTTTT+TGG + chr8.4:15121968-15121987 MS.gene47020:CDS 40.0%
! TCCTTGGTTGTTATTCTTGC+TGG + chr8.4:15121696-15121715 MS.gene47020:CDS 40.0%
! TGATTCCGGTTTTACACTCA+CGG + chr8.4:15121740-15121759 MS.gene47020:CDS 40.0%
!! AGTCTTGGGTTGAATACTTG+GGG + chr8.4:15121834-15121853 MS.gene47020:CDS 40.0%
!! GAAAGTAGTCTTGTTGGTGA+TGG + chr8.4:15122263-15122282 MS.gene47020:CDS 40.0%
!! GAGTCTTGGGTTGAATACTT+GGG + chr8.4:15121833-15121852 MS.gene47020:CDS 40.0%
!! GGTTATGTTGCTACTGATTC+CGG + chr8.4:15121726-15121745 MS.gene47020:CDS 40.0%
!! TGAGTCTTGGGTTGAATACT+TGG + chr8.4:15121832-15121851 MS.gene47020:CDS 40.0%
!!! TCACTCGGTGTTTACCTTTT+AGG + chr8.4:15121450-15121469 MS.gene47020:CDS 40.0%
ACCGATTACTAAGCCACTTG+TGG - chr8.4:15121331-15121350 None:intergenic 45.0%
AGCAAACAAACCAAACCAGC+TGG - chr8.4:15122149-15122168 None:intergenic 45.0%
CTTTATCAGCAATCGGTGAC+TGG + chr8.4:15122191-15122210 MS.gene47020:CDS 45.0%
GCCACAAGTGGCTTAGTAAT+CGG + chr8.4:15121327-15121346 MS.gene47020:CDS 45.0%
TATCAACGTTAGCGTGACGA+AGG - chr8.4:15121581-15121600 None:intergenic 45.0%
! AAGAAGAAAACGAGTGCTGC+AGG - chr8.4:15121534-15121553 None:intergenic 45.0%
! AGAAGAAAACGAGTGCTGCA+GGG - chr8.4:15121533-15121552 None:intergenic 45.0%
!! CGATGTGAATGCGTTTTTGG+CGG + chr8.4:15121971-15121990 MS.gene47020:CDS 45.0%
!! GTTATTCTTGCTGGTGCTGT+TGG + chr8.4:15121705-15121724 MS.gene47020:CDS 45.0%
AACCCCAGTAACGGTAAACG+CGG - chr8.4:15122069-15122088 None:intergenic 50.0%
ACTCCCAGTACTCGATCTCA+AGG - chr8.4:15121946-15121965 None:intergenic 50.0%
ACTGCCTTGAGATCGAGTAC+TGG + chr8.4:15121939-15121958 MS.gene47020:CDS 50.0%
AGCGAGAGCAACAAGAAGAG+GGG - chr8.4:15121628-15121647 None:intergenic 50.0%
CAACAACACTACGCCACAAG+TGG + chr8.4:15121315-15121334 MS.gene47020:CDS 50.0%
CAGCAATCGGTGACTGGAAA+TGG + chr8.4:15122197-15122216 MS.gene47020:CDS 50.0%
CAGCGAGAGCAACAAGAAGA+GGG - chr8.4:15121629-15121648 None:intergenic 50.0%
CTACCGCGTTTACCGTTACT+GGG + chr8.4:15122063-15122082 MS.gene47020:CDS 50.0%
CTGCCTTGAGATCGAGTACT+GGG + chr8.4:15121940-15121959 MS.gene47020:CDS 50.0%
GACTGGAAATGGTTCGCAAC+AGG + chr8.4:15122208-15122227 MS.gene47020:CDS 50.0%
GATAAGCATGCTAGTCCAGC+TGG + chr8.4:15122131-15122150 MS.gene47020:CDS 50.0%
GTGACGAAGGAGATTGGTGT+TGG - chr8.4:15121568-15121587 None:intergenic 50.0%
GTTAGCGTGACGAAGGAGAT+TGG - chr8.4:15121574-15121593 None:intergenic 50.0%
TACCGCGTTTACCGTTACTG+GGG + chr8.4:15122064-15122083 MS.gene47020:CDS 50.0%
TCAGCGAGAGCAACAAGAAG+AGG - chr8.4:15121630-15121649 None:intergenic 50.0%
TGGAAATGGTTCGCAACAGG+CGG + chr8.4:15122211-15122230 MS.gene47020:CDS 50.0%
CTCGCTGATACTGCGTTTCG+CGG + chr8.4:15121642-15121661 MS.gene47020:CDS 55.0%
GCATGCTAGTCCAGCTGGTT+TGG + chr8.4:15122136-15122155 MS.gene47020:CDS 55.0%
GCTACCGCGTTTACCGTTAC+TGG + chr8.4:15122062-15122081 MS.gene47020:CDS 55.0%
TGGTTCGCAACAGGCGGTTG+TGG + chr8.4:15122217-15122236 MS.gene47020:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 15121294 15122334 15121294 ID=MS.gene47020
chr8.4 mRNA 15121294 15122334 15121294 ID=MS.gene47020.t1;Parent=MS.gene47020
chr8.4 exon 15121294 15122334 15121294 ID=MS.gene47020.t1.exon1;Parent=MS.gene47020.t1
chr8.4 CDS 15121294 15122334 15121294 ID=cds.MS.gene47020.t1;Parent=MS.gene47020.t1
Gene Sequence

>MS.gene47020

ATGTCATCAACAATATTGAAACAACAACACTACGCCACAAGTGGCTTAGTAATCGGTTACGCACTCTGTTCAAGCTTATTAGCAATCATAAACAAATACGCTATTACTCAATTCAACTACCCAGGTCTTTTAACAGCCTTACAATATCTCACTTCTTCACTCGGTGTTTACCTTTTAGGTAAATTAGGGTTTCTTCATCACGATCCTTTCACTATTCCAATCGCTAAGAAATTCTTCCCTGCAGCACTCGTTTTCTTCTTAGCGATCTTCACCAACACCAATCTCCTTCGTCACGCTAACGTTGATACGTTCATTGTTTTCAGATCTCTTACCCCTCTTCTTGTTGCTCTCGCTGATACTGCGTTTCGCGGTCAACCCTCTCCGTCTAATTTTACCTTTTTGTCCTTGGTTGTTATTCTTGCTGGTGCTGTTGGTTATGTTGCTACTGATTCCGGTTTTACACTCACGGCTTATTCTTGGGCTTTTGCTTATTTGGTTACCATTACTACTGAGATGGTTTATATTAAGCATATGGTTATGAGTCTTGGGTTGAATACTTGGGGTTTTGTGCTTTATAATAATGTATTGTCATTGATGATTGCTCCTGTTTTTTGGTTTTTGACTGGTGAGAATTTTGAGGTTTTTACTGCCTTGAGATCGAGTACTGGGAGTTTGTTCGATGTGAATGCGTTTTTGGCGGTTTCGTTGTCTTGTGTGTTTGGTTTGTTGATTAGTTTCTTTGGATTTGCTGCTAGAAAAGCTGTTTCTGCTACCGCGTTTACCGTTACTGGGGTTGTGAATAAGTTTCTGACTGTGGCAATTAATGTGACGATTTGGGATAAGCATGCTAGTCCAGCTGGTTTGGTTTGTTTGCTTTTTACGATTATTGGGGGTGTTCTTTATCAGCAATCGGTGACTGGAAATGGTTCGCAACAGGCGGTTGTGGTGACTAAACAACAGTCTGATGTTGAAAGTAGTCTTGTTGGTGATGGTGAATCTGAAGATGAGAGTGAAGGAAAGGGTAAACATGCTTCTCTATGA

Protein sequence

>MS.gene47020.t1

MSSTILKQQHYATSGLVIGYALCSSLLAIINKYAITQFNYPGLLTALQYLTSSLGVYLLGKLGFLHHDPFTIPIAKKFFPAALVFFLAIFTNTNLLRHANVDTFIVFRSLTPLLVALADTAFRGQPSPSNFTFLSLVVILAGAVGYVATDSGFTLTAYSWAFAYLVTITTEMVYIKHMVMSLGLNTWGFVLYNNVLSLMIAPVFWFLTGENFEVFTALRSSTGSLFDVNAFLAVSLSCVFGLLISFFGFAARKAVSATAFTVTGVVNKFLTVAINVTIWDKHASPAGLVCLLFTIIGGVLYQQSVTGNGSQQAVVVTKQQSDVESSLVGDGESEDESEGKGKHASL