Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47065.t1 | XP_003618792.1 | 97.5 | 157 | 4 | 0 | 1 | 157 | 1 | 157 | 5.60E-79 | 303.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47065.t1 | Q0WPX7 | 48.1 | 108 | 55 | 1 | 38 | 144 | 15 | 122 | 6.6e-20 | 98.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47065.t1 | G7KN78 | 97.5 | 157 | 4 | 0 | 1 | 157 | 1 | 157 | 4.0e-79 | 303.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051234 | MS.gene47065 | 0.811325 | 7.41E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47065.t1 | MTR_6g022020 | 97.452 | 157 | 4 | 0 | 1 | 157 | 1 | 157 | 8.12e-111 | 311 |
| MS.gene47065.t1 | MTR_6g022040 | 87.975 | 158 | 14 | 3 | 1 | 157 | 1 | 154 | 2.06e-92 | 265 |
| MS.gene47065.t1 | MTR_0086s0010 | 41.379 | 145 | 77 | 4 | 19 | 155 | 11 | 155 | 1.74e-27 | 105 |
| MS.gene47065.t1 | MTR_7g069610 | 46.296 | 108 | 55 | 1 | 32 | 136 | 13 | 120 | 1.99e-26 | 99.8 |
| MS.gene47065.t1 | MTR_1g101440 | 43.519 | 108 | 57 | 2 | 37 | 140 | 21 | 128 | 1.18e-24 | 97.4 |
| MS.gene47065.t1 | MTR_2g018860 | 37.500 | 128 | 72 | 2 | 33 | 152 | 25 | 152 | 1.50e-23 | 92.8 |
| MS.gene47065.t1 | MTR_4g102740 | 43.810 | 105 | 57 | 1 | 38 | 140 | 32 | 136 | 1.30e-22 | 90.9 |
| MS.gene47065.t1 | MTR_4g017810 | 51.220 | 82 | 40 | 0 | 58 | 139 | 26 | 107 | 3.04e-18 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47065.t1 | AT5G14360 | 54.386 | 171 | 56 | 5 | 1 | 157 | 1 | 163 | 2.17e-54 | 169 |
| MS.gene47065.t1 | AT5G40630 | 46.914 | 162 | 65 | 4 | 1 | 142 | 1 | 161 | 9.24e-43 | 139 |
| MS.gene47065.t1 | AT5G62100 | 49.057 | 106 | 53 | 1 | 38 | 142 | 15 | 120 | 1.22e-26 | 98.2 |
| MS.gene47065.t1 | AT5G62100 | 49.057 | 106 | 53 | 1 | 38 | 142 | 15 | 120 | 1.22e-26 | 98.2 |
| MS.gene47065.t1 | AT5G62100 | 48.148 | 108 | 55 | 1 | 38 | 144 | 15 | 122 | 7.43e-26 | 97.8 |
| MS.gene47065.t1 | AT5G62100 | 48.148 | 108 | 55 | 1 | 38 | 144 | 15 | 122 | 1.03e-25 | 97.8 |
| MS.gene47065.t1 | AT5G62100 | 48.148 | 108 | 55 | 1 | 38 | 144 | 15 | 122 | 6.49e-25 | 97.4 |
| MS.gene47065.t1 | AT5G62100 | 48.148 | 108 | 55 | 1 | 38 | 144 | 15 | 122 | 1.16e-24 | 96.7 |
| MS.gene47065.t1 | AT5G07220 | 42.017 | 119 | 68 | 1 | 38 | 155 | 21 | 139 | 1.23e-24 | 96.7 |
| MS.gene47065.t1 | AT5G52060 | 41.667 | 108 | 58 | 1 | 38 | 140 | 39 | 146 | 1.18e-21 | 89.4 |
| MS.gene47065.t1 | AT3G51780 | 41.071 | 112 | 50 | 2 | 36 | 131 | 8 | 119 | 1.95e-19 | 82.4 |
Find 29 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGAAGCAACTTCAACTTT+TGG | 0.327143 | 6.3:-15396179 | MS.gene47065:intron |
| TAGAGAAGCCTTTGTTCTCT+TGG | 0.329320 | 6.3:+15395369 | None:intergenic |
| AGCATATCTAAACTTGGCTT+TGG | 0.330269 | 6.3:-15396371 | MS.gene47065:CDS |
| TTCTTCACATGATTGGTGTT+AGG | 0.341495 | 6.3:-15395317 | MS.gene47065:CDS |
| TTTGTCACTTGTAACAAGTT+TGG | 0.343072 | 6.3:-15395394 | MS.gene47065:CDS |
| AATTTCAGGAGAATTGAAAA+TGG | 0.354607 | 6.3:-15395418 | MS.gene47065:intron |
| AGAAATGAAGCTACTTGGTT+TGG | 0.373066 | 6.3:-15395253 | MS.gene47065:CDS |
| TCACTACAATCCTTCTCAAT+TGG | 0.382631 | 6.3:+15396329 | None:intergenic |
| AGCTTCATTTCTTTGATAGC+TGG | 0.393914 | 6.3:+15395264 | None:intergenic |
| TCTTCACATGATTGGTGTTA+GGG | 0.396279 | 6.3:-15395316 | MS.gene47065:CDS |
| AATGAATTTCTTCACATGAT+TGG | 0.414769 | 6.3:-15395324 | MS.gene47065:CDS |
| ATCAAAGAAATGAAGCTACT+TGG | 0.456169 | 6.3:-15395258 | MS.gene47065:CDS |
| AGAGAGAGTAACAAAAGTGT+TGG | 0.471291 | 6.3:-15396254 | MS.gene47065:CDS |
| TGTAGAAGCATATCTAAACT+TGG | 0.485899 | 6.3:-15396377 | MS.gene47065:CDS |
| TGAAAATAGTGAAATCGAAT+GGG | 0.493131 | 6.3:-15396309 | MS.gene47065:CDS |
| GTGAAAATAGTGAAATCGAA+TGG | 0.497284 | 6.3:-15396310 | MS.gene47065:CDS |
| AAGCTACTTGGTTTGGCAAG+AGG | 0.530918 | 6.3:-15395246 | MS.gene47065:CDS |
| TATACTCTGATTGTGTAACT+AGG | 0.541450 | 6.3:+15395207 | None:intergenic |
| ATCGAATGGGAACTTAGGCC+TGG | 0.546005 | 6.3:-15396296 | MS.gene47065:CDS |
| AAAATAAGATGATGAAGTTG+AGG | 0.552877 | 6.3:-15396412 | None:intergenic |
| TGATGAAGTTGAGGTCAAAG+AGG | 0.559715 | 6.3:-15396403 | MS.gene47065:CDS |
| GAACAAAGGCTTCTCTACAA+AGG | 0.591704 | 6.3:-15395363 | MS.gene47065:CDS |
| AGACAAGGTTCTTCTTCTAG+AGG | 0.592443 | 6.3:-15395289 | MS.gene47065:CDS |
| GAGTTTCAACTATGTCAAAG+TGG | 0.599297 | 6.3:-15396214 | MS.gene47065:CDS |
| AGTAGCATCTCCAATTGAGA+AGG | 0.608005 | 6.3:-15396339 | MS.gene47065:CDS |
| GTGAAATCGAATGGGAACTT+AGG | 0.612081 | 6.3:-15396301 | MS.gene47065:CDS |
| TGGTGTTAGGGACAAAGACA+AGG | 0.622779 | 6.3:-15395304 | MS.gene47065:CDS |
| GTTTGGAGCCAAGAGAACAA+AGG | 0.661395 | 6.3:-15395377 | MS.gene47065:CDS |
| GAATGGGAACTTAGGCCTGG+TGG | 0.713354 | 6.3:-15396293 | MS.gene47065:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAATATAAATAAAAATTT+TGG | + | chr6.3:15396065-15396084 | None:intergenic | 0.0% |
| !! | TAAATTGAACAATTATAATT+AGG | - | chr6.3:15395796-15395815 | MS.gene47065:intron | 10.0% |
| !! | AAAATTTATGAATGAATTTC+AGG | - | chr6.3:15396178-15396197 | MS.gene47065:intron | 15.0% |
| !! | AAACTATAAAAATTACTTTG+AGG | - | chr6.3:15396143-15396162 | MS.gene47065:intron | 15.0% |
| !! | AAATTATCCTTATTAACTAT+TGG | + | chr6.3:15395673-15395692 | None:intergenic | 15.0% |
| !! | AATAGTTAATAAGGATAATT+TGG | - | chr6.3:15395672-15395691 | MS.gene47065:intron | 15.0% |
| !! | TTATTAAGATCAAATCATTT+AGG | - | chr6.3:15395928-15395947 | MS.gene47065:intron | 15.0% |
| !!! | CATTTTGAAACAAATTTTTT+TGG | + | chr6.3:15395546-15395565 | None:intergenic | 15.0% |
| !!! | TAGTTTTAAAAGTAATACTT+TGG | + | chr6.3:15395649-15395668 | None:intergenic | 15.0% |
| !! | AAACTAACCAATAGTTAATA+AGG | - | chr6.3:15395663-15395682 | MS.gene47065:intron | 20.0% |
| !! | AGAAAAAGTTATTATTGCAT+TGG | + | chr6.3:15395586-15395605 | None:intergenic | 20.0% |
| ! | AAATAATTCAAAGTTCGGTT+AGG | + | chr6.3:15395975-15395994 | None:intergenic | 25.0% |
| ! | AATGAATTTCTTCACATGAT+TGG | - | chr6.3:15396286-15396305 | MS.gene47065:CDS | 25.0% |
| ! | AATTTCAGGAGAATTGAAAA+TGG | - | chr6.3:15396192-15396211 | MS.gene47065:CDS | 25.0% |
| ! | GGAGTAAATAATTCAAAGTT+CGG | + | chr6.3:15395980-15395999 | None:intergenic | 25.0% |
| ! | TGAAAATAGTGAAATCGAAT+GGG | - | chr6.3:15395301-15395320 | MS.gene47065:CDS | 25.0% |
| !!! | AACTACTTGCTAACAATTTT+CGG | + | chr6.3:15395888-15395907 | None:intergenic | 25.0% |
| AACCCTGTAACTCTTAAAAA+TGG | + | chr6.3:15396001-15396020 | None:intergenic | 30.0% | |
| AATCAATACAAATGAAAGGC+TGG | + | chr6.3:15395467-15395486 | None:intergenic | 30.0% | |
| AGGCAATCAATACAAATGAA+AGG | + | chr6.3:15395471-15395490 | None:intergenic | 30.0% | |
| ATCAAAGAAATGAAGCTACT+TGG | - | chr6.3:15396352-15396371 | MS.gene47065:CDS | 30.0% | |
| GTGAAAATAGTGAAATCGAA+TGG | - | chr6.3:15395300-15395319 | MS.gene47065:CDS | 30.0% | |
| GTTACAGGGTTAAACAAATT+TGG | - | chr6.3:15396010-15396029 | MS.gene47065:intron | 30.0% | |
| TATACTCTGATTGTGTAACT+AGG | + | chr6.3:15396406-15396425 | None:intergenic | 30.0% | |
| ! | ATTGAAGCAACTTCAACTTT+TGG | - | chr6.3:15395431-15395450 | MS.gene47065:intron | 30.0% |
| ! | GAGTAGACAATAACTTTTGT+TGG | - | chr6.3:15396032-15396051 | MS.gene47065:intron | 30.0% |
| ! | GTAGTGTGCAAAGTATTAAT+TGG | + | chr6.3:15395624-15395643 | None:intergenic | 30.0% |
| ! | TGTAGAAGCATATCTAAACT+TGG | - | chr6.3:15395233-15395252 | MS.gene47065:CDS | 30.0% |
| ! | TGTGCAAAGTATTAATTGGA+GGG | + | chr6.3:15395620-15395639 | None:intergenic | 30.0% |
| ! | TTTGTCACTTGTAACAAGTT+TGG | - | chr6.3:15396216-15396235 | MS.gene47065:CDS | 30.0% |
| !! | AAACGACACTCATTTTGAAA+CGG | - | chr6.3:15395755-15395774 | MS.gene47065:intron | 30.0% |
| !! | ACTCCATTTTTAAGAGTTAC+AGG | - | chr6.3:15395995-15396014 | MS.gene47065:intron | 30.0% |
| !! | CTCCATTTTTAAGAGTTACA+GGG | - | chr6.3:15395996-15396015 | MS.gene47065:intron | 30.0% |
| !! | TATTGATTGCCTTTTGTTTC+GGG | - | chr6.3:15395479-15395498 | MS.gene47065:intron | 30.0% |
| !!! | GTATTGATTGCCTTTTGTTT+CGG | - | chr6.3:15395478-15395497 | MS.gene47065:intron | 30.0% |
| !!! | TTCAAAATGAGTGTCGTTTT+AGG | + | chr6.3:15395754-15395773 | None:intergenic | 30.0% |
| AGAAATGAAGCTACTTGGTT+TGG | - | chr6.3:15396357-15396376 | MS.gene47065:CDS | 35.0% | |
| AGCATATCTAAACTTGGCTT+TGG | - | chr6.3:15395239-15395258 | MS.gene47065:CDS | 35.0% | |
| GAGTTTCAACTATGTCAAAG+TGG | - | chr6.3:15395396-15395415 | MS.gene47065:CDS | 35.0% | |
| TAATTCAAAGTTCGGTTAGG+AGG | + | chr6.3:15395972-15395991 | None:intergenic | 35.0% | |
| TAGTTTGAACCCGAAACAAA+AGG | + | chr6.3:15395491-15395510 | None:intergenic | 35.0% | |
| TCACTACAATCCTTCTCAAT+TGG | + | chr6.3:15395284-15395303 | None:intergenic | 35.0% | |
| ! | AGAGAGAGTAACAAAAGTGT+TGG | - | chr6.3:15395356-15395375 | MS.gene47065:CDS | 35.0% |
| ! | AGCTTCATTTCTTTGATAGC+TGG | + | chr6.3:15396349-15396368 | None:intergenic | 35.0% |
| ! | GTGTGCAAAGTATTAATTGG+AGG | + | chr6.3:15395621-15395640 | None:intergenic | 35.0% |
| ! | TTCTTCACATGATTGGTGTT+AGG | - | chr6.3:15396293-15396312 | MS.gene47065:CDS | 35.0% |
| !! | AAGCGACACTCATTTTGAAA+CGG | + | chr6.3:15395520-15395539 | None:intergenic | 35.0% |
| !! | TCTTCACATGATTGGTGTTA+GGG | - | chr6.3:15396294-15396313 | MS.gene47065:CDS | 35.0% |
| AGACAAGGTTCTTCTTCTAG+AGG | - | chr6.3:15396321-15396340 | MS.gene47065:CDS | 40.0% | |
| AGTAGCATCTCCAATTGAGA+AGG | - | chr6.3:15395271-15395290 | MS.gene47065:CDS | 40.0% | |
| GAACAAAGGCTTCTCTACAA+AGG | - | chr6.3:15396247-15396266 | MS.gene47065:CDS | 40.0% | |
| GTGAAATCGAATGGGAACTT+AGG | - | chr6.3:15395309-15395328 | MS.gene47065:CDS | 40.0% | |
| TAGAGAAGCCTTTGTTCTCT+TGG | + | chr6.3:15396244-15396263 | None:intergenic | 40.0% | |
| TGATGAAGTTGAGGTCAAAG+AGG | - | chr6.3:15395207-15395226 | MS.gene47065:CDS | 40.0% | |
| !!! | GACACTCATTTTGAAACGGA+GGG | - | chr6.3:15395759-15395778 | MS.gene47065:intron | 40.0% |
| !!! | GACACTCATTTTGAAACGGA+GGG | + | chr6.3:15395778-15395759 | None:intergenic | 40.0% |
| !!! | GCACACACGTAAGACTTTTT+TGG | + | chr6.3:15396115-15396134 | None:intergenic | 40.0% |
| ! | GTTTGGAGCCAAGAGAACAA+AGG | - | chr6.3:15396233-15396252 | MS.gene47065:CDS | 45.0% |
| ! | TGGTGTTAGGGACAAAGACA+AGG | - | chr6.3:15396306-15396325 | MS.gene47065:CDS | 45.0% |
| ! | TTTTGTACAAGCATGCCACC+AGG | + | chr6.3:15395335-15395354 | None:intergenic | 45.0% |
| !! | AAGCTACTTGGTTTGGCAAG+AGG | - | chr6.3:15396364-15396383 | MS.gene47065:CDS | 45.0% |
| !!! | CGACACTCATTTTGAAACGG+AGG | - | chr6.3:15395758-15395777 | MS.gene47065:intron | 45.0% |
| !!! | CGACACTCATTTTGAAACGG+AGG | + | chr6.3:15395777-15395758 | None:intergenic | 45.0% |
| ATCGAATGGGAACTTAGGCC+TGG | - | chr6.3:15395314-15395333 | MS.gene47065:CDS | 50.0% | |
| CGGTTAGGAGGTAACGAAAC+TGG | + | chr6.3:15395960-15395979 | None:intergenic | 50.0% | |
| GAATGGGAACTTAGGCCTGG+TGG | - | chr6.3:15395317-15395336 | MS.gene47065:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 15395206 | 15396426 | 15395206 | ID=MS.gene47065 |
| chr6.3 | mRNA | 15395206 | 15396426 | 15395206 | ID=MS.gene47065.t1;Parent=MS.gene47065 |
| chr6.3 | exon | 15396180 | 15396426 | 15396180 | ID=MS.gene47065.t1.exon1;Parent=MS.gene47065.t1 |
| chr6.3 | CDS | 15396180 | 15396426 | 15396180 | ID=cds.MS.gene47065.t1;Parent=MS.gene47065.t1 |
| chr6.3 | exon | 15395206 | 15395432 | 15395206 | ID=MS.gene47065.t1.exon2;Parent=MS.gene47065.t1 |
| chr6.3 | CDS | 15395206 | 15395432 | 15395206 | ID=cds.MS.gene47065.t1;Parent=MS.gene47065.t1 |
| Gene Sequence |
| Protein sequence |
