Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47499.t1 | AES90241.1 | 100 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.10E-20 | 107.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47499.t1 | G7JMI7 | 100.0 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 1.5e-20 | 107.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052500 | MS.gene47499 | 0.81458 | 1.43E-51 | -1.69E-46 |
| MS.gene052502 | MS.gene47499 | 0.805537 | 1.27E-49 | -1.69E-46 |
| MS.gene052826 | MS.gene47499 | 0.8062 | 9.23E-50 | -1.69E-46 |
| MS.gene052954 | MS.gene47499 | 0.837657 | 4.57E-57 | -1.69E-46 |
| MS.gene057392 | MS.gene47499 | 0.831862 | 1.31E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47499.t1 | MTR_4g087080 | 100.000 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 5.15e-35 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACGGTTGATTGGTGAAAA+AGG | 0.267840 | 4.3:+25972154 | None:intergenic |
| AATACTCCTCTCCCTTTCTA+TGG | 0.290250 | 4.3:-25972130 | MS.gene47499:CDS |
| AATAGAATTGTGCATATCTA+TGG | 0.361157 | 4.3:-25972248 | MS.gene47499:CDS |
| AACTGCCATAACAAATTCTA+TGG | 0.401714 | 4.3:+25972216 | None:intergenic |
| GGAGTATTTGCACGGTTGAT+TGG | 0.424542 | 4.3:+25972145 | None:intergenic |
| AATGGCCATAGAATTTGTTA+TGG | 0.435872 | 4.3:-25972221 | MS.gene47499:CDS |
| GTGCATATCTATGGTTAGAA+TGG | 0.466421 | 4.3:-25972239 | MS.gene47499:CDS |
| CAACGGAGGTAACCATAGAA+AGG | 0.524840 | 4.3:+25972118 | None:intergenic |
| AACGGAGGTAACCATAGAAA+GGG | 0.543015 | 4.3:+25972119 | None:intergenic |
| AGGTAACCATAGAAAGGGAG+AGG | 0.582749 | 4.3:+25972124 | None:intergenic |
| AAGGGAGAGGAGTATTTGCA+CGG | 0.631175 | 4.3:+25972137 | None:intergenic |
| AATTCAATTCAACTCAACGG+AGG | 0.680829 | 4.3:+25972104 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAGAATTGTGCATATCTA+TGG | - | chr4.3:25972131-25972150 | MS.gene47499:CDS | 25.0% |
| AACTGCCATAACAAATTCTA+TGG | + | chr4.3:25972166-25972185 | None:intergenic | 30.0% | |
| !! | AATGGCCATAGAATTTGTTA+TGG | - | chr4.3:25972158-25972177 | MS.gene47499:CDS | 30.0% |
| GTGCATATCTATGGTTAGAA+TGG | - | chr4.3:25972140-25972159 | MS.gene47499:CDS | 35.0% | |
| AACGGAGGTAACCATAGAAA+GGG | + | chr4.3:25972263-25972282 | None:intergenic | 40.0% | |
| AATACTCCTCTCCCTTTCTA+TGG | - | chr4.3:25972249-25972268 | MS.gene47499:CDS | 40.0% | |
| AGGTAACCATAGAAAGGGAG+AGG | + | chr4.3:25972258-25972277 | None:intergenic | 45.0% | |
| CAACGGAGGTAACCATAGAA+AGG | + | chr4.3:25972264-25972283 | None:intergenic | 45.0% | |
| GCACGGTTGATTGGTGAAAA+AGG | + | chr4.3:25972228-25972247 | None:intergenic | 45.0% | |
| ! | GGAGTATTTGCACGGTTGAT+TGG | + | chr4.3:25972237-25972256 | None:intergenic | 45.0% |
| !! | AAGGGAGAGGAGTATTTGCA+CGG | + | chr4.3:25972245-25972264 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 25972117 | 25972284 | 25972117 | ID=MS.gene47499 |
| chr4.3 | mRNA | 25972117 | 25972284 | 25972117 | ID=MS.gene47499.t1;Parent=MS.gene47499 |
| chr4.3 | exon | 25972117 | 25972284 | 25972117 | ID=MS.gene47499.t1.exon1;Parent=MS.gene47499.t1 |
| chr4.3 | CDS | 25972117 | 25972284 | 25972117 | ID=cds.MS.gene47499.t1;Parent=MS.gene47499.t1 |
| Gene Sequence |
| Protein sequence |
