Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47617.t1 | XP_003620232.2 | 84.9 | 53 | 8 | 0 | 60 | 112 | 57 | 109 | 4.80E-16 | 94 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47617.t1 | G7KL61 | 84.9 | 53 | 8 | 0 | 60 | 112 | 57 | 109 | 3.5e-16 | 94.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05264 | MS.gene47617 | 0.869559 | 2.83E-66 | -1.69E-46 |
| MS.gene05265 | MS.gene47617 | 0.800166 | 1.64E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47617.t1 | MTR_6g078940 | 84.906 | 53 | 8 | 0 | 60 | 112 | 57 | 109 | 3.48e-26 | 94.7 |
| MS.gene47617.t1 | MTR_6g077630 | 43.443 | 122 | 39 | 2 | 20 | 112 | 30 | 150 | 2.60e-23 | 88.6 |
| MS.gene47617.t1 | MTR_6g079130 | 43.443 | 122 | 39 | 2 | 20 | 112 | 82 | 202 | 1.92e-22 | 87.4 |
| MS.gene47617.t1 | MTR_6g477680 | 57.317 | 82 | 8 | 1 | 58 | 112 | 353 | 434 | 4.63e-21 | 86.7 |
| MS.gene47617.t1 | MTR_6g077680 | 57.317 | 82 | 8 | 1 | 58 | 112 | 435 | 516 | 6.78e-21 | 86.7 |
| MS.gene47617.t1 | MTR_6g077680 | 56.962 | 79 | 7 | 1 | 58 | 109 | 435 | 513 | 2.80e-19 | 82.0 |
| MS.gene47617.t1 | MTR_6g077630 | 46.316 | 95 | 21 | 2 | 38 | 103 | 48 | 141 | 6.59e-18 | 74.3 |
| MS.gene47617.t1 | MTR_6g078950 | 62.963 | 54 | 18 | 1 | 59 | 112 | 52 | 103 | 3.96e-15 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAAGAAGTTGGTTGTTTC+TGG | 0.129847 | 6.1:-70391023 | MS.gene47617:CDS |
| TGAAGAACAGATTGAGATTT+TGG | 0.155235 | 6.1:-70391069 | MS.gene47617:CDS |
| AATGATGATTTAGTTGAAAA+TGG | 0.241490 | 6.1:-70391249 | MS.gene47617:intron |
| AATGTGATTGACTCTATATT+TGG | 0.259312 | 6.1:-70391182 | MS.gene47617:intron |
| AAGATCTCCCCTCCACATTC+TGG | 0.300473 | 6.1:-70391482 | MS.gene47617:CDS |
| TCCTTCATCATTGGTGGATC+TGG | 0.321529 | 6.1:-70391207 | MS.gene47617:CDS |
| CAAACAGAAGGAAACGTTAT+AGG | 0.348276 | 6.1:-70391547 | MS.gene47617:CDS |
| CATCAAGAATGTGTCAGATT+TGG | 0.357757 | 6.1:-70390706 | MS.gene47617:CDS |
| AAGAGGTGTCAGTGCTATAC+AGG | 0.404532 | 6.1:-70391450 | MS.gene47617:intron |
| AACTTCTTGCAAAGCCAGTC+TGG | 0.423623 | 6.1:+70391035 | None:intergenic |
| TGGAAAAGATTGTGCCAGAC+TGG | 0.443412 | 6.1:-70391049 | MS.gene47617:CDS |
| TATAGCACTGACACCTCTTA+AGG | 0.443464 | 6.1:+70391454 | None:intergenic |
| CATTCTGGATACGCCTTAAG+AGG | 0.445628 | 6.1:-70391467 | MS.gene47617:CDS |
| GTGGATTGGATAACAACATT+TGG | 0.460085 | 6.1:+70391513 | None:intergenic |
| GATCTTATGTACTGGTGGAT+TGG | 0.469560 | 6.1:+70391499 | None:intergenic |
| GACTGCAAATCCTTCATCAT+TGG | 0.473819 | 6.1:-70391216 | MS.gene47617:CDS |
| TGCAAATCCTTCATCATTGG+TGG | 0.512374 | 6.1:-70391213 | MS.gene47617:CDS |
| GGCAGAAAGAATGCAGAAAG+AGG | 0.522697 | 6.1:-70391570 | MS.gene47617:CDS |
| AGACTGGCTTTGCAAGAAGT+TGG | 0.532090 | 6.1:-70391033 | MS.gene47617:CDS |
| ACCAGATCCACCAATGATGA+AGG | 0.533226 | 6.1:+70391206 | None:intergenic |
| TTTCTGGAGACACTATGTAC+TGG | 0.547965 | 6.1:-70391007 | MS.gene47617:intron |
| TGCAGAAAGAGGCAAACAGA+AGG | 0.574957 | 6.1:-70391559 | MS.gene47617:CDS |
| AGTTGAAAATGGATATTCTG+AGG | 0.585036 | 6.1:-70391238 | MS.gene47617:CDS |
| GGGGAGATCTTATGTACTGG+TGG | 0.586694 | 6.1:+70391494 | None:intergenic |
| AGGCGTATCCAGAATGTGGA+GGG | 0.589280 | 6.1:+70391474 | None:intergenic |
| CTTAAGGCGTATCCAGAATG+TGG | 0.599320 | 6.1:+70391470 | None:intergenic |
| GGAGGGGAGATCTTATGTAC+TGG | 0.605142 | 6.1:+70391491 | None:intergenic |
| AAGGCGTATCCAGAATGTGG+AGG | 0.651219 | 6.1:+70391473 | None:intergenic |
| GGCGTATCCAGAATGTGGAG+GGG | 0.654858 | 6.1:+70391475 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTCTTAGTAATAAACATT+CGG | - | chr6.1:70391148-70391167 | MS.gene47617:intron | 15.0% |
| !!! | TTTATGCTTGTTATTTATTA+TGG | - | chr6.1:70391381-70391400 | MS.gene47617:intron | 15.0% |
| !! | AATGATGATTTAGTTGAAAA+TGG | - | chr6.1:70391024-70391043 | MS.gene47617:CDS | 20.0% |
| !! | TTTCTTAGTAATAAACATTC+GGG | - | chr6.1:70391149-70391168 | MS.gene47617:intron | 20.0% |
| ! | TTTGAAAATTGTCTCTTGTA+TGG | - | chr6.1:70390846-70390865 | MS.gene47617:intron | 25.0% |
| !! | AATGTGATTGACTCTATATT+TGG | - | chr6.1:70391091-70391110 | MS.gene47617:intron | 25.0% |
| !! | TAGAATATCAGAACTTTTGA+TGG | - | chr6.1:70391427-70391446 | MS.gene47617:intron | 25.0% |
| !!! | TTTAGAGTATCAGTCTTTTA+GGG | - | chr6.1:70391329-70391348 | MS.gene47617:intron | 25.0% |
| !!! | TTTTAGAGTATCAGTCTTTT+AGG | - | chr6.1:70391328-70391347 | MS.gene47617:intron | 25.0% |
| AGTTGAAAATGGATATTCTG+AGG | - | chr6.1:70391035-70391054 | MS.gene47617:CDS | 30.0% | |
| ! | CTGATACTCTAAAAGAACAA+AGG | + | chr6.1:70391322-70391341 | None:intergenic | 30.0% |
| ! | TGAAGAACAGATTGAGATTT+TGG | - | chr6.1:70391204-70391223 | MS.gene47617:CDS | 30.0% |
| !! | TACTTTTAAGTAAATGCCAG+AGG | + | chr6.1:70391481-70391500 | None:intergenic | 30.0% |
| !!! | AAGTTGAGCAATCTTTTTTG+AGG | + | chr6.1:70390988-70391007 | None:intergenic | 30.0% |
| AATAGTAGAACCTAGCAGTT+TGG | - | chr6.1:70391404-70391423 | MS.gene47617:intron | 35.0% | |
| CAAACAGAAGGAAACGTTAT+AGG | - | chr6.1:70390726-70390745 | MS.gene47617:intron | 35.0% | |
| CATCAAGAATGTGTCAGATT+TGG | - | chr6.1:70391567-70391586 | MS.gene47617:CDS | 35.0% | |
| GGGTCACAAATCACTAAATA+AGG | + | chr6.1:70391453-70391472 | None:intergenic | 35.0% | |
| TGATATTCTACCAAACTGCT+AGG | + | chr6.1:70391417-70391436 | None:intergenic | 35.0% | |
| ! | AAGTAAATGCCAGAGGATTT+TGG | + | chr6.1:70391474-70391493 | None:intergenic | 35.0% |
| ! | AGTAAATGCCAGAGGATTTT+GGG | + | chr6.1:70391473-70391492 | None:intergenic | 35.0% |
| ! | GCTTAAAACACCAGACTTTT+GGG | + | chr6.1:70390966-70390985 | None:intergenic | 35.0% |
| !! | GTGGATTGGATAACAACATT+TGG | + | chr6.1:70390763-70390782 | None:intergenic | 35.0% |
| AAAATGACTGCCCAAAAGTC+TGG | - | chr6.1:70390953-70390972 | MS.gene47617:intron | 40.0% | |
| ACTAATCCTGTCCTCTCAAT+AGG | - | chr6.1:70391175-70391194 | MS.gene47617:intron | 40.0% | |
| ATTTGTGACCCAAAATCCTC+TGG | - | chr6.1:70391462-70391481 | MS.gene47617:CDS | 40.0% | |
| GACTGCAAATCCTTCATCAT+TGG | - | chr6.1:70391057-70391076 | MS.gene47617:CDS | 40.0% | |
| TGCAAATCCTTCATCATTGG+TGG | - | chr6.1:70391060-70391079 | MS.gene47617:CDS | 40.0% | |
| ! | ACACCTGAGAGATTGTTGTT+CGG | - | chr6.1:70390911-70390930 | MS.gene47617:intron | 40.0% |
| ! | GATCTTATGTACTGGTGGAT+TGG | + | chr6.1:70390777-70390796 | None:intergenic | 40.0% |
| ! | GGCTTAAAACACCAGACTTT+TGG | + | chr6.1:70390967-70390986 | None:intergenic | 40.0% |
| ! | TGCAAGAAGTTGGTTGTTTC+TGG | - | chr6.1:70391250-70391269 | MS.gene47617:intron | 40.0% |
| ! | TTTCTGGAGACACTATGTAC+TGG | - | chr6.1:70391266-70391285 | MS.gene47617:intron | 40.0% |
| !! | TATAGCACTGACACCTCTTA+AGG | + | chr6.1:70390822-70390841 | None:intergenic | 40.0% |
| !! | TTTTGGGCAGTCATTTTCTG+AGG | + | chr6.1:70390950-70390969 | None:intergenic | 40.0% |
| AACTTCTTGCAAAGCCAGTC+TGG | + | chr6.1:70391241-70391260 | None:intergenic | 45.0% | |
| ACCAGATCCACCAATGATGA+AGG | + | chr6.1:70391070-70391089 | None:intergenic | 45.0% | |
| CATTCTGGATACGCCTTAAG+AGG | - | chr6.1:70390806-70390825 | MS.gene47617:intron | 45.0% | |
| CTTAAGGCGTATCCAGAATG+TGG | + | chr6.1:70390806-70390825 | None:intergenic | 45.0% | |
| CTTCAGCTTCTCCTATTGAG+AGG | + | chr6.1:70391189-70391208 | None:intergenic | 45.0% | |
| GAACCGAACAACAATCTCTC+AGG | + | chr6.1:70390917-70390936 | None:intergenic | 45.0% | |
| GGCAGAAAGAATGCAGAAAG+AGG | - | chr6.1:70390703-70390722 | MS.gene47617:CDS | 45.0% | |
| TGCAGAAAGAGGCAAACAGA+AGG | - | chr6.1:70390714-70390733 | MS.gene47617:intron | 45.0% | |
| TGGAAAAGATTGTGCCAGAC+TGG | - | chr6.1:70391224-70391243 | MS.gene47617:CDS | 45.0% | |
| ! | TCCTTCATCATTGGTGGATC+TGG | - | chr6.1:70391066-70391085 | MS.gene47617:CDS | 45.0% |
| ! | TGGAGACACTATGTACTGGT+GGG | - | chr6.1:70391270-70391289 | MS.gene47617:intron | 45.0% |
| !! | AAGAGGTGTCAGTGCTATAC+AGG | - | chr6.1:70390823-70390842 | MS.gene47617:intron | 45.0% |
| !! | AGACTGGCTTTGCAAGAAGT+TGG | - | chr6.1:70391240-70391259 | MS.gene47617:intron | 45.0% |
| !! | GTATCAGTCTTTTAGGGACG+TGG | - | chr6.1:70391335-70391354 | MS.gene47617:intron | 45.0% |
| !! | TCTCAGGTGTTGACTATGCA+GGG | + | chr6.1:70390901-70390920 | None:intergenic | 45.0% |
| AAGATCTCCCCTCCACATTC+TGG | - | chr6.1:70390791-70390810 | MS.gene47617:intron | 50.0% | |
| AAGGCGTATCCAGAATGTGG+AGG | + | chr6.1:70390803-70390822 | None:intergenic | 50.0% | |
| AGGCGTATCCAGAATGTGGA+GGG | + | chr6.1:70390802-70390821 | None:intergenic | 50.0% | |
| CTGGAGACACTATGTACTGG+TGG | - | chr6.1:70391269-70391288 | MS.gene47617:intron | 50.0% | |
| GCTTCTCCTATTGAGAGGAC+AGG | + | chr6.1:70391184-70391203 | None:intergenic | 50.0% | |
| GGAGGGGAGATCTTATGTAC+TGG | + | chr6.1:70390785-70390804 | None:intergenic | 50.0% | |
| GGGGAGATCTTATGTACTGG+TGG | + | chr6.1:70390782-70390801 | None:intergenic | 50.0% | |
| !! | CTCAGGTGTTGACTATGCAG+GGG | + | chr6.1:70390900-70390919 | None:intergenic | 50.0% |
| !! | CTCTCAGGTGTTGACTATGC+AGG | + | chr6.1:70390902-70390921 | None:intergenic | 50.0% |
| GGCGTATCCAGAATGTGGAG+GGG | + | chr6.1:70390801-70390820 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 70390701 | 70391594 | 70390701 | ID=MS.gene47617 |
| chr6.1 | mRNA | 70390701 | 70391594 | 70390701 | ID=MS.gene47617.t1;Parent=MS.gene47617 |
| chr6.1 | exon | 70391451 | 70391594 | 70391451 | ID=MS.gene47617.t1.exon1;Parent=MS.gene47617.t1 |
| chr6.1 | CDS | 70391451 | 70391594 | 70391451 | ID=cds.MS.gene47617.t1;Parent=MS.gene47617.t1 |
| chr6.1 | exon | 70391183 | 70391258 | 70391183 | ID=MS.gene47617.t1.exon2;Parent=MS.gene47617.t1 |
| chr6.1 | CDS | 70391183 | 70391258 | 70391183 | ID=cds.MS.gene47617.t1;Parent=MS.gene47617.t1 |
| chr6.1 | exon | 70391008 | 70391098 | 70391008 | ID=MS.gene47617.t1.exon3;Parent=MS.gene47617.t1 |
| chr6.1 | CDS | 70391008 | 70391098 | 70391008 | ID=cds.MS.gene47617.t1;Parent=MS.gene47617.t1 |
| chr6.1 | exon | 70390701 | 70390728 | 70390701 | ID=MS.gene47617.t1.exon4;Parent=MS.gene47617.t1 |
| chr6.1 | CDS | 70390701 | 70390728 | 70390701 | ID=cds.MS.gene47617.t1;Parent=MS.gene47617.t1 |
| Gene Sequence |
| Protein sequence |
