Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052527 | MS.gene47767 | 0.811348 | 7.32E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTTACATGGGAGGATTAA+AGG | 0.234605 | 8.1:+51707826 | None:intergenic |
| CAGTATTTGCCGGTAGAATC+AGG | 0.358742 | 8.1:-51708382 | None:intergenic |
| AACTCTAGCAGGCTCTTTCC+AGG | 0.361101 | 8.1:-51707988 | None:intergenic |
| GATAGACTTGCAGTATTTGC+CGG | 0.410071 | 8.1:-51708392 | None:intergenic |
| GTCTATCACAAAATCAAAGA+AGG | 0.465270 | 8.1:+51708408 | MS.gene47767:CDS |
| CAGATGCTAGAGAATGAGCC+TGG | 0.507226 | 8.1:+51707970 | MS.gene47767:intron |
| AGATACTTGCCTGATTCTAC+CGG | 0.514786 | 8.1:+51708373 | MS.gene47767:CDS |
| GTCTGTCGCTACTACTCTTG+AGG | 0.550215 | 8.1:+51708013 | MS.gene47767:CDS |
| GCGACAGACGAAACTCTAGC+AGG | 0.588210 | 8.1:-51707999 | None:intergenic |
| TCTATCACAAAATCAAAGAA+GGG | 0.593364 | 8.1:+51708409 | MS.gene47767:CDS |
| ACTACATAACTTTACATGGG+AGG | 0.625525 | 8.1:+51707818 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTTAATATTATTGATCA+TGG | + | chr8.1:51708481-51708500 | MS.gene47767:intron | 10.0% |
| !! | TAGGTCAAATTTGAAAAATT+AGG | - | chr8.1:51708077-51708096 | None:intergenic | 20.0% |
| !!! | ATGGTCTTTTTTATATTTTC+AGG | + | chr8.1:51708500-51708519 | MS.gene47767:intron | 20.0% |
| ! | AGTATCAATGCTACATATAT+AGG | - | chr8.1:51708096-51708115 | None:intergenic | 25.0% |
| ! | TCTATCACAAAATCAAAGAA+GGG | + | chr8.1:51708409-51708428 | MS.gene47767:CDS | 25.0% |
| CACGTGTTAGTAAATACAAT+TGG | + | chr8.1:51708281-51708300 | MS.gene47767:intron | 30.0% | |
| GAACAGATTAAACTGTTAGA+AGG | + | chr8.1:51708342-51708361 | MS.gene47767:intron | 30.0% | |
| GTCTATCACAAAATCAAAGA+AGG | + | chr8.1:51708408-51708427 | MS.gene47767:CDS | 30.0% | |
| TAGAATTCAGAAGAAGAATC+AGG | + | chr8.1:51707876-51707895 | MS.gene47767:intron | 30.0% | |
| GGATGCAGTTATAACAACAA+TGG | + | chr8.1:51707908-51707927 | MS.gene47767:intron | 35.0% | |
| !! | AGTTGATGATGTTGGTTCTT+TGG | + | chr8.1:51707936-51707955 | MS.gene47767:intron | 35.0% |
| !! | TGGTATTCAGTTGATGATGT+TGG | + | chr8.1:51707928-51707947 | MS.gene47767:intron | 35.0% |
| ACTCTTGAGGTAAGCATCTT+CGG | + | chr8.1:51708026-51708045 | MS.gene47767:intron | 40.0% | |
| AGATACTTGCCTGATTCTAC+CGG | + | chr8.1:51708373-51708392 | MS.gene47767:CDS | 40.0% | |
| GAGGATTAAAGGTAAGTGCT+TGG | + | chr8.1:51707837-51707856 | MS.gene47767:intron | 40.0% | |
| GATAGACTTGCAGTATTTGC+CGG | - | chr8.1:51708395-51708414 | None:intergenic | 40.0% | |
| !! | AGAAGAATCAGGTTGACTGT+TGG | + | chr8.1:51707887-51707906 | MS.gene47767:intron | 40.0% |
| CAGTATTTGCCGGTAGAATC+AGG | - | chr8.1:51708385-51708404 | None:intergenic | 45.0% | |
| AACTCTAGCAGGCTCTTTCC+AGG | - | chr8.1:51707991-51708010 | None:intergenic | 50.0% | |
| CAGATGCTAGAGAATGAGCC+TGG | + | chr8.1:51707970-51707989 | MS.gene47767:intron | 50.0% | |
| GTCGACATGATTCTCACACG+TGG | + | chr8.1:51708145-51708164 | MS.gene47767:intron | 50.0% | |
| GTCTGTCGCTACTACTCTTG+AGG | + | chr8.1:51708013-51708032 | MS.gene47767:CDS | 50.0% | |
| GCGACAGACGAAACTCTAGC+AGG | - | chr8.1:51708002-51708021 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 51707833 | 51708530 | 51707833 | ID=MS.gene47767 |
| chr8.1 | mRNA | 51707833 | 51708530 | 51707833 | ID=MS.gene47767.t1;Parent=MS.gene47767 |
| chr8.1 | exon | 51707833 | 51707847 | 51707833 | ID=MS.gene47767.t1.exon1;Parent=MS.gene47767.t1 |
| chr8.1 | CDS | 51707833 | 51707847 | 51707833 | ID=cds.MS.gene47767.t1;Parent=MS.gene47767.t1 |
| chr8.1 | exon | 51707973 | 51708034 | 51707973 | ID=MS.gene47767.t1.exon2;Parent=MS.gene47767.t1 |
| chr8.1 | CDS | 51707973 | 51708034 | 51707973 | ID=cds.MS.gene47767.t1;Parent=MS.gene47767.t1 |
| chr8.1 | exon | 51708361 | 51708430 | 51708361 | ID=MS.gene47767.t1.exon3;Parent=MS.gene47767.t1 |
| chr8.1 | CDS | 51708361 | 51708430 | 51708361 | ID=cds.MS.gene47767.t1;Parent=MS.gene47767.t1 |
| chr8.1 | exon | 51708522 | 51708530 | 51708522 | ID=MS.gene47767.t1.exon4;Parent=MS.gene47767.t1 |
| chr8.1 | CDS | 51708522 | 51708530 | 51708522 | ID=cds.MS.gene47767.t1;Parent=MS.gene47767.t1 |
| Gene Sequence |
| Protein sequence |
