AlfalfaGEDB | MS.gene052527

M. sativa cultivar XinJiangDaYe / MS.gene052527

KEGG: K19986
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052527.t1	RHN82447.1	82.9	35	6	0	18	52	488	522	6.20E-09	69.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052527.t1	A0A072W1S7	82.9	35	6	0	18	52	575	609	4.4e-09	69.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052527	MS.gene06131	0.809174	2.16E-50	-1.69E-46
MS.gene052527	MS.gene068166	0.805207	1.49E-49	-1.69E-46
MS.gene052527	MS.gene070222	0.831503	1.61E-55	-1.69E-46
MS.gene052527	MS.gene07101	0.810431	1.16E-50	-1.69E-46
MS.gene052527	MS.gene072057	0.802341	5.88E-49	-1.69E-46
MS.gene052527	MS.gene07339	0.808955	2.40E-50	-1.69E-46
MS.gene052527	MS.gene073564	0.802209	6.25E-49	-1.69E-46
MS.gene052527	MS.gene07608	0.801555	8.52E-49	-1.69E-46
MS.gene052527	MS.gene08889	0.804178	2.45E-49	-1.69E-46
MS.gene052527	MS.gene24597	0.81655	5.22E-52	-1.69E-46
MS.gene052527	MS.gene25475	0.817119	3.89E-52	-1.69E-46
MS.gene052527	MS.gene26426	0.817411	3.34E-52	-1.69E-46
MS.gene052527	MS.gene29229	0.80717	5.76E-50	-1.69E-46
MS.gene052527	MS.gene30792	-0.808071	3.71E-50	-1.69E-46
MS.gene052527	MS.gene39222	0.825718	4.02E-54	-1.69E-46
MS.gene052527	MS.gene40028	0.805562	1.26E-49	-1.69E-46
MS.gene052527	MS.gene42625	0.803955	2.72E-49	-1.69E-46
MS.gene052527	MS.gene46392	0.827599	1.43E-54	-1.69E-46
MS.gene052527	MS.gene46447	0.818246	2.17E-52	-1.69E-46
MS.gene052527	MS.gene47767	0.811348	7.32E-51	-1.69E-46
MS.gene052527	MS.gene50005	0.809841	1.55E-50	-1.69E-46
MS.gene052527	MS.gene51445	0.8029	4.51E-49	-1.69E-46
MS.gene052527	MS.gene51515	0.803298	3.73E-49	-1.69E-46
MS.gene052527	MS.gene54555	0.807482	4.95E-50	-1.69E-46
MS.gene052527	MS.gene55062	0.808384	3.18E-50	-1.69E-46
MS.gene052527	MS.gene62943	0.805851	1.09E-49	-1.69E-46
MS.gene052527	MS.gene65412	0.828072	1.10E-54	-1.69E-46
MS.gene052527	MS.gene66089	0.810713	1.01E-50	-1.69E-46
MS.gene052527	MS.gene67107	0.825496	4.54E-54	-1.69E-46
MS.gene052527	MS.gene69649	0.80153	8.62E-49	-1.69E-46
MS.gene052527	MS.gene70325	0.803053	4.19E-49	-1.69E-46
MS.gene052527	MS.gene76046	0.807795	4.24E-50	-1.69E-46
MS.gene052527	MS.gene87429	0.8191	1.39E-52	-1.69E-46
MS.gene052527	MS.gene87972	0.80023	1.59E-48	-1.69E-46
MS.gene052527	MS.gene88976	0.80848	3.03E-50	-1.69E-46
MS.gene052527	MS.gene89677	0.807051	6.11E-50	-1.69E-46
MS.gene052527	MS.gene92557	0.8001	1.69E-48	-1.69E-46
MS.gene052527	MS.gene94992	0.807927	3.98E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052527.t1	MTR_1g111120	82.857	35	6	0	18	52	575	609	6.84e-17	73.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 17 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGAATGCTCAAATGTATT+TGG	0.176146	4.3:+23373229	MS.gene052527:CDS
TTTGGAGGTTAATTTCTATT+TGG	0.214852	4.3:+23373174	MS.gene052527:CDS
TTCTATTTGGCCATTGTTCT+TGG	0.292592	4.3:+23373187	MS.gene052527:CDS
TGAAACAGGACAAACTGTTT+TGG	0.303724	4.3:+23373890	MS.gene052527:CDS
TGCTCAAATGTATTTGGATA+TGG	0.360208	4.3:+23373235	MS.gene052527:CDS
CCATTGTTCTTGGCAGAAGA+TGG	0.389900	4.3:+23373197	MS.gene052527:CDS
ATTGGAGAAGGAAACCATTC+AGG	0.390426	4.3:-23373275	None:intergenic
GGGTCCTCTCGGCCTTCAAC+AGG	0.392881	4.3:+23373911	MS.gene052527:CDS
ATTTGGATATGGAGGACAAT+GGG	0.404111	4.3:+23373246	MS.gene052527:CDS
TACATTTGAGCATTCAATAG+TGG	0.454695	4.3:-23373224	None:intergenic
TGAACTCATATACCTGTTGA+AGG	0.495646	4.3:-23373923	None:intergenic
TATTTGGATATGGAGGACAA+TGG	0.581621	4.3:+23373245	MS.gene052527:CDS
CCATCTTCTGCCAAGAACAA+TGG	0.595327	4.3:-23373197	None:intergenic
TTTGGATATGGAGGACAATG+GGG	0.604747	4.3:+23373247	MS.gene052527:CDS
TCAAATGTATTTGGATATGG+AGG	0.605535	4.3:+23373238	MS.gene052527:CDS
ACAATGGGGAGATACCTGAA+TGG	0.664243	4.3:+23373261	MS.gene052527:CDS
TATACCTGTTGAAGGCCGAG+AGG	0.715952	4.3:-23373915	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAATTAAAAAGAAAATTAC+AGG	-	chr4.3:23373053-23373072	None:intergenic	15.0%
!!!	AATTTATCTGAATTTGTTTA+TGG	+	chr4.3:23373004-23373023	MS.gene052527:intron	15.0%
!!!	ACTTTTTGATTAAGATTTTT+GGG	+	chr4.3:23373860-23373879	MS.gene052527:intron	15.0%
!!	AAATAAGAAGAAGAGAAAAA+AGG	-	chr4.3:23372972-23372991	None:intergenic	20.0%
!!	AGTGATAGTAATATAATCTA+TGG	+	chr4.3:23373412-23373431	MS.gene052527:intron	20.0%
!!	CAAGCATGATATATAAAATT+AGG	-	chr4.3:23373127-23373146	None:intergenic	20.0%
!!!	CTAATTTTATATATCATGCT+TGG	+	chr4.3:23373125-23373144	MS.gene052527:intron	20.0%
!!!	GACTTTTTGATTAAGATTTT+TGG	+	chr4.3:23373859-23373878	MS.gene052527:intron	20.0%
!	ATTGAATGCTCAAATGTATT+TGG	+	chr4.3:23373229-23373248	MS.gene052527:CDS	25.0%
!	CATTTCATATATTACTGTTC+AGG	+	chr4.3:23373724-23373743	MS.gene052527:intron	25.0%
!	TGTTTGTTTACCTGAAAAAT+TGG	-	chr4.3:23373296-23373315	None:intergenic	25.0%
!	TTTGGAGGTTAATTTCTATT+TGG	+	chr4.3:23373174-23373193	MS.gene052527:CDS	25.0%
!!!	TTTTTCAGGTAAACAAACAA+AGG	+	chr4.3:23373297-23373316	MS.gene052527:intron	25.0%
	ATGTCTTCAATATCAAGTCT+CGG	+	chr4.3:23373677-23373696	MS.gene052527:intron	30.0%
	ATTCACTTCCAAACATAAGA+TGG	-	chr4.3:23373082-23373101	None:intergenic	30.0%
	GTATTAGTCCATCTTATGTT+TGG	+	chr4.3:23373071-23373090	MS.gene052527:intron	30.0%
	TACATTTGAGCATTCAATAG+TGG	-	chr4.3:23373227-23373246	None:intergenic	30.0%
	TCAAATGTATTTGGATATGG+AGG	+	chr4.3:23373238-23373257	MS.gene052527:CDS	30.0%
	TGCTCAAATGTATTTGGATA+TGG	+	chr4.3:23373235-23373254	MS.gene052527:CDS	30.0%
	TTGAATGTATCCACGTAAAT+TGG	+	chr4.3:23373622-23373641	MS.gene052527:intron	30.0%
	TTTACCTGAAAAATTGGAGA+AGG	-	chr4.3:23373290-23373309	None:intergenic	30.0%
	TTTGAATGTATTGCATGCAT+TGG	+	chr4.3:23373509-23373528	MS.gene052527:intron	30.0%
	AAGTGTAGAGCTATAACCAA+TGG	-	chr4.3:23373336-23373355	None:intergenic	35.0%
	ATCTGATGCATTTGTGTGAA+GGG	+	chr4.3:23373784-23373803	MS.gene052527:intron	35.0%
	ATTTGGATATGGAGGACAAT+GGG	+	chr4.3:23373246-23373265	MS.gene052527:CDS	35.0%
	TATTTGGATATGGAGGACAA+TGG	+	chr4.3:23373245-23373264	MS.gene052527:CDS	35.0%
	TTAAAGCTTACCAGAATTGC+AGG	+	chr4.3:23373755-23373774	MS.gene052527:intron	35.0%
	TTCTATTTGGCCATTGTTCT+TGG	+	chr4.3:23373187-23373206	MS.gene052527:CDS	35.0%
!	GAAACAGGACAAACTGTTTT+GGG	+	chr4.3:23373891-23373910	MS.gene052527:CDS	35.0%
!	GTTTCCTTCTCCAATTTTTC+AGG	+	chr4.3:23373283-23373302	MS.gene052527:CDS	35.0%
!	TATATCATGCTTGGTTCTCT+TGG	+	chr4.3:23373134-23373153	MS.gene052527:intron	35.0%
!	TGAAACAGGACAAACTGTTT+TGG	+	chr4.3:23373890-23373909	MS.gene052527:CDS	35.0%
!!!	GTAGTTTTGACGAACATGTT+TGG	+	chr4.3:23373156-23373175	MS.gene052527:intron	35.0%
	AGGATACTTCATGCTTCCAT+TGG	+	chr4.3:23373317-23373336	MS.gene052527:intron	40.0%
	ATTGGAGAAGGAAACCATTC+AGG	-	chr4.3:23373278-23373297	None:intergenic	40.0%
	CATCTGATGCATTTGTGTGA+AGG	+	chr4.3:23373783-23373802	MS.gene052527:intron	40.0%
	GTATCCACGTAAATTGGTGT+CGG	+	chr4.3:23373628-23373647	MS.gene052527:intron	40.0%
	TGAACACAACAATGCACAGA+CGG	+	chr4.3:23373559-23373578	MS.gene052527:intron	40.0%
	TTTGGATATGGAGGACAATG+GGG	+	chr4.3:23373247-23373266	MS.gene052527:CDS	40.0%
!	TATCCACGTAAATTGGTGTC+GGG	+	chr4.3:23373629-23373648	MS.gene052527:intron	40.0%
!	TGTATTGCATGCATTGGTGT+CGG	+	chr4.3:23373515-23373534	MS.gene052527:intron	40.0%
!!	GTTTTGACGAACATGTTTGG+AGG	+	chr4.3:23373159-23373178	MS.gene052527:intron	40.0%
!!	TCGGTGCTAGATAGAGTATA+AGG	+	chr4.3:23373696-23373715	MS.gene052527:intron	40.0%
	ACAATGGGGAGATACCTGAA+TGG	+	chr4.3:23373261-23373280	MS.gene052527:CDS	45.0%
	AGATGCAATGCCTGCAATTC+TGG	-	chr4.3:23373768-23373787	None:intergenic	45.0%
	CCATCTTCTGCCAAGAACAA+TGG	-	chr4.3:23373200-23373219	None:intergenic	45.0%
	CCATTGTTCTTGGCAGAAGA+TGG	+	chr4.3:23373197-23373216	MS.gene052527:CDS	45.0%
!	TTTTGGGCTGACGTTGAAAC+AGG	+	chr4.3:23373876-23373895	MS.gene052527:CDS	45.0%
!!	CAAACTGTTTTGGGTCCTCT+CGG	+	chr4.3:23373900-23373919	MS.gene052527:CDS	45.0%
	GTGCCCGACACCAATTTACG+TGG	-	chr4.3:23373635-23373654	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	23372940	23373932	23372940	ID=MS.gene052527
chr4.3	mRNA	23372940	23373932	23372940	ID=MS.gene052527.t1;Parent=MS.gene052527
chr4.3	exon	23372940	23372950	23372940	ID=MS.gene052527.t1.exon1;Parent=MS.gene052527.t1
chr4.3	CDS	23372940	23372950	23372940	ID=cds.MS.gene052527.t1;Parent=MS.gene052527.t1
chr4.3	exon	23373160	23373304	23373160	ID=MS.gene052527.t1.exon2;Parent=MS.gene052527.t1
chr4.3	CDS	23373160	23373304	23373160	ID=cds.MS.gene052527.t1;Parent=MS.gene052527.t1
chr4.3	exon	23373874	23373932	23373874	ID=MS.gene052527.t1.exon3;Parent=MS.gene052527.t1
chr4.3	CDS	23373874	23373932	23373874	ID=cds.MS.gene052527.t1;Parent=MS.gene052527.t1

Gene Sequence

>MS.gene052527

ATGATTTTCATGTAAGGATTTGATGTTAACCTTTTTTCTCTTCTTCTTATTTATGTGATTAAATAATTTATCTGAATTTGTTTATGGTTTCTTCAGTTTATGATTTTGCTCCTGTAATTTTCTTTTTAATTGTATTAGTCCATCTTATGTTTGGAAGTGAATTGAGAATTTAAATAGTAGTTCTCCTAATTTTATATATCATGCTTGGTTCTCTTGGTAGTTTTGACGAACATGTTTGGAGGTTAATTTCTATTTGGCCATTGTTCTTGGCAGAAGATGGAGTACCACTATTGAATGCTCAAATGTATTTGGATATGGAGGACAATGGGGAGATACCTGAATGGTTTCCTTCTCCAATTTTTCAGGTAAACAAACAAAGGATACTTCATGCTTCCATTGGTTATAGCTCTACACTTCTAATTTGTTATGTTGTAATAGCATGTTTATATATCACATGCATTCATATAAACATAGTGATAGTAATATAATCTATGGCACACAAACATTGAACACGACAGTGACACTGACACATAGACATATTAGTAATATAATTTGCGAAAAGAGAAGTGTTTGAATGTATTGCATGCATTGGTGTCGGACACTGACATAAGTCAGACATTGAACACAACAATGCACAGACGGACATATATATGTCAATAATAATTTAAAAAAATAGAAGTGATTGAATGTATCCACGTAAATTGGTGTCGGGCACTAAAATGTGTCAGACACTGTACATGTCTTCAATATCAAGTCTCGGTGCTAGATAGAGTATAAGGTTGATCATTTCATATATTACTGTTCAGGAGCTTTTTTTAAAGCTTACCAGAATTGCAGGCATTGCATCTGATGCATTTGTGTGAAGGGAAATATTTGCAACTATTTTATTGATGAGACTTGCAGAAAGCAGTGATACTATGACTTTTTGATTAAGATTTTTGGGCTGACGTTGAAACAGGACAAACTGTTTTGGGTCCTCTCGGCCTTCAACAG

Protein sequence

>MS.gene052527.t1

MIFIFDEHVWRLISIWPLFLAEDGVPLLNAQMYLDMEDNGEIPEWFPSPIFQIFGLTLKQDKLFWVLSAFN