Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48264.t1 | RHN40111.1 | 84.4 | 64 | 9 | 1 | 1 | 64 | 176 | 238 | 2.10E-16 | 94.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48264.t1 | A0A396GIW2 | 84.4 | 64 | 9 | 1 | 1 | 64 | 176 | 238 | 1.5e-16 | 94.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058266 | MS.gene48264 | 0.848044 | 7.87E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTCTGTGGCAACTCTTAC+AGG | 0.225907 | 4.2:+77197787 | None:intergenic |
| TCAACCTTACCATCACATTT+TGG | 0.252049 | 4.2:+77197829 | None:intergenic |
| ATCTCCTGAGCTAAGATGTT+GGG | 0.371891 | 4.2:+77197674 | None:intergenic |
| TCACTACCATCTTCAATTAC+TGG | 0.376171 | 4.2:+77197727 | None:intergenic |
| TAGTGATGATGAAGATGTTC+AGG | 0.431216 | 4.2:-77197710 | MS.gene48264:CDS |
| GATGAAGACCCTAGGGTTGA+TGG | 0.460558 | 4.2:-77197754 | MS.gene48264:CDS |
| AACACCAAAATGTGATGGTA+AGG | 0.512205 | 4.2:-77197833 | MS.gene48264:CDS |
| CTGTAAGAGTTGCCACAGAA+GGG | 0.536965 | 4.2:-77197786 | MS.gene48264:CDS |
| TGTAAGAGTTGCCACAGAAG+GGG | 0.544300 | 4.2:-77197785 | MS.gene48264:CDS |
| ACTGGCTCACCATCAACCCT+AGG | 0.552430 | 4.2:+77197745 | None:intergenic |
| GAGATCTTGATGAAGACCCT+AGG | 0.554127 | 4.2:-77197762 | MS.gene48264:CDS |
| CCTGTAAGAGTTGCCACAGA+AGG | 0.558919 | 4.2:-77197787 | MS.gene48264:CDS |
| ACTCTTACAGGAGGAGTGAT+CGG | 0.576759 | 4.2:+77197799 | None:intergenic |
| GATCTCCTGAGCTAAGATGT+TGG | 0.579131 | 4.2:+77197673 | None:intergenic |
| TCTCCTGAGCTAAGATGTTG+GGG | 0.586803 | 4.2:+77197675 | None:intergenic |
| CCAGAAACACCAAAATGTGA+TGG | 0.595828 | 4.2:-77197838 | MS.gene48264:CDS |
| AGATCTTGATGAAGACCCTA+GGG | 0.607768 | 4.2:-77197761 | MS.gene48264:CDS |
| GATGATGAAGATGTTCAGGA+TGG | 0.628053 | 4.2:-77197706 | MS.gene48264:CDS |
| GGTGAGCCAGTAATTGAAGA+TGG | 0.629717 | 4.2:-77197733 | MS.gene48264:CDS |
| GTAAGAGTTGCCACAGAAGG+GGG | 0.631031 | 4.2:-77197784 | MS.gene48264:CDS |
| CTGGCTCACCATCAACCCTA+GGG | 0.637088 | 4.2:+77197746 | None:intergenic |
| TCTGTGGCAACTCTTACAGG+AGG | 0.691602 | 4.2:+77197790 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACACCAAAATGTGATGGTA+AGG | - | chr4.2:77197707-77197726 | MS.gene48264:CDS | 35.0% | |
| TCACTACCATCTTCAATTAC+TGG | + | chr4.2:77197816-77197835 | None:intergenic | 35.0% | |
| ! | TAGTGATGATGAAGATGTTC+AGG | - | chr4.2:77197830-77197849 | MS.gene48264:CDS | 35.0% |
| ! | TCAACCTTACCATCACATTT+TGG | + | chr4.2:77197714-77197733 | None:intergenic | 35.0% |
| AGATCTTGATGAAGACCCTA+GGG | - | chr4.2:77197779-77197798 | MS.gene48264:CDS | 40.0% | |
| CCAGAAACACCAAAATGTGA+TGG | - | chr4.2:77197702-77197721 | MS.gene48264:CDS | 40.0% | |
| GATGATGAAGATGTTCAGGA+TGG | - | chr4.2:77197834-77197853 | MS.gene48264:CDS | 40.0% | |
| !!! | CCATCACATTTTGGTGTTTC+TGG | + | chr4.2:77197705-77197724 | None:intergenic | 40.0% |
| ACTCTTACAGGAGGAGTGAT+CGG | + | chr4.2:77197744-77197763 | None:intergenic | 45.0% | |
| CTGTAAGAGTTGCCACAGAA+GGG | - | chr4.2:77197754-77197773 | MS.gene48264:CDS | 45.0% | |
| GAGATCTTGATGAAGACCCT+AGG | - | chr4.2:77197778-77197797 | MS.gene48264:CDS | 45.0% | |
| GGTGAGCCAGTAATTGAAGA+TGG | - | chr4.2:77197807-77197826 | MS.gene48264:CDS | 45.0% | |
| TGTAAGAGTTGCCACAGAAG+GGG | - | chr4.2:77197755-77197774 | MS.gene48264:CDS | 45.0% | |
| ATCAAGATCTCCCCCTTCTG+TGG | + | chr4.2:77197769-77197788 | None:intergenic | 50.0% | |
| CCTGTAAGAGTTGCCACAGA+AGG | - | chr4.2:77197753-77197772 | MS.gene48264:CDS | 50.0% | |
| CCTTCTGTGGCAACTCTTAC+AGG | + | chr4.2:77197756-77197775 | None:intergenic | 50.0% | |
| GATGAAGACCCTAGGGTTGA+TGG | - | chr4.2:77197786-77197805 | MS.gene48264:CDS | 50.0% | |
| GTAAGAGTTGCCACAGAAGG+GGG | - | chr4.2:77197756-77197775 | MS.gene48264:CDS | 50.0% | |
| TCTGTGGCAACTCTTACAGG+AGG | + | chr4.2:77197753-77197772 | None:intergenic | 50.0% | |
| CTGGCTCACCATCAACCCTA+GGG | + | chr4.2:77197797-77197816 | None:intergenic | 55.0% | |
| ! | ACTGGCTCACCATCAACCCT+AGG | + | chr4.2:77197798-77197817 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 77197684 | 77197878 | 77197684 | ID=MS.gene48264 |
| chr4.2 | mRNA | 77197684 | 77197878 | 77197684 | ID=MS.gene48264.t1;Parent=MS.gene48264 |
| chr4.2 | exon | 77197684 | 77197878 | 77197684 | ID=MS.gene48264.t1.exon1;Parent=MS.gene48264.t1 |
| chr4.2 | CDS | 77197684 | 77197878 | 77197684 | ID=cds.MS.gene48264.t1;Parent=MS.gene48264.t1 |
| Gene Sequence |
| Protein sequence |
