AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058266

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058266.t1 MTR_2g033580 100.000 36 0 0 200 235 265 300 6.00e-18 81.3
MS.gene058266.t1 MTR_2g033520 78.947 38 8 0 200 237 262 299 4.76e-14 70.5
MS.gene058266.t1 MTR_2g033550 77.143 35 8 0 200 234 260 294 3.92e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058266.t1 AT1G47980 85.714 35 5 0 200 234 275 309 1.88e-13 68.9

Find 67 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCTGTGGCAACTCTTAC+AGG 0.225907 2.2:+55193106 None:intergenic
ACCATAGCTTCCTAGCTTTC+CGG 0.232650 2.2:+55192177 None:intergenic
TCAACCTTACCATCACATTT+TGG 0.252049 2.2:+55193148 None:intergenic
ATACCTAGAATTTGCTTTGC+AGG 0.275664 2.2:+55192215 None:intergenic
ATCTCCTGAGCTGAGATGTT+GGG 0.302898 2.2:+55192993 None:intergenic
GAAATATTGAGGATTATATA+TGG 0.348872 2.2:-55191055 MS.gene058266:CDS
TCTGCTGAGTTTAAAGTGAT+TGG 0.364681 2.2:+55192076 None:intergenic
TCACTACCATCTTCAATTAC+TGG 0.376171 2.2:+55193046 None:intergenic
GGCCCTGCAAAGCAAATTCT+AGG 0.381337 2.2:-55192218 MS.gene058266:CDS
ATACGACCATCTGCTCCCTT+AGG 0.398738 2.2:+55190998 None:intergenic
CAACATGAAGGGTGCTAAAC+CGG 0.428786 2.2:-55192114 MS.gene058266:CDS
TTGGCTAACGACGTGCTAAC+CGG 0.430696 2.2:+55192095 None:intergenic
TAGTGATGATGAAGATGTTC+AGG 0.431216 2.2:-55193029 MS.gene058266:CDS
CCCCGGTGGCTTCTACCCTA+AGG 0.448904 2.2:-55191014 MS.gene058266:CDS
TCCCTTAGGGTAGAAGCCAC+CGG 0.458454 2.2:+55191012 None:intergenic
GATGAAGACCCTAGGGTTGA+TGG 0.460558 2.2:-55193073 MS.gene058266:CDS
ATACAGATGTACTCCGAACT+AGG 0.472343 2.2:+55192938 None:intergenic
TACCTAGAATTTGCTTTGCA+GGG 0.475894 2.2:+55192216 None:intergenic
GAACTAGGGTACCTGGTAGA+TGG 0.478163 2.2:+55192953 None:intergenic
GATCTCCTGAGCTGAGATGT+TGG 0.479025 2.2:+55192992 None:intergenic
ACAACAACAGTATGTGGAAC+GGG 0.482220 2.2:-55192150 MS.gene058266:CDS
TATGATTGGTGATTTGAAGA+AGG 0.483320 2.2:-55192273 MS.gene058266:CDS
TACCTTACTCCTCAGCAGTT+GGG 0.487695 2.2:-55192007 MS.gene058266:CDS
TACGACCATCTGCTCCCTTA+GGG 0.488464 2.2:+55190999 None:intergenic
TACATCTGTATCACAGATGA+AGG 0.494690 2.2:-55192926 MS.gene058266:CDS
CCCTTAGGGTAGAAGCCACC+GGG 0.500034 2.2:+55191013 None:intergenic
CCCGGTGGCTTCTACCCTAA+GGG 0.503709 2.2:-55191013 MS.gene058266:CDS
AATCCCCAACATCTCAGCTC+AGG 0.503790 2.2:-55192997 MS.gene058266:CDS
ATCTACCAGGTACCCTAGTT+CGG 0.508012 2.2:-55192951 MS.gene058266:CDS
CTCCCAACTGCTGAGGAGTA+AGG 0.509251 2.2:+55192005 None:intergenic
AACACCAAAATGTGATGGTA+AGG 0.512205 2.2:-55193152 MS.gene058266:CDS
ATAATCCTCAATATTTCTTG+TGG 0.512721 2.2:+55191061 None:intergenic
TCTCCTGAGCTGAGATGTTG+GGG 0.523063 2.2:+55192994 None:intergenic
GGACAAGACAAGGATATGAT+TGG 0.527499 2.2:-55192287 MS.gene058266:CDS
CTGTAAGAGTTGCCACAGAA+GGG 0.536965 2.2:-55193105 MS.gene058266:CDS
GTACCTTACTCCTCAGCAGT+TGG 0.538964 2.2:-55192008 MS.gene058266:CDS
ACCGGAAAGCTAGGAAGCTA+TGG 0.539638 2.2:-55192178 MS.gene058266:CDS
TTGAGGATTATATATGGAAG+TGG 0.540356 2.2:-55191049 MS.gene058266:CDS
TCTAGAAAGATTCAACATGA+AGG 0.544066 2.2:-55192126 MS.gene058266:CDS
TGTAAGAGTTGCCACAGAAG+GGG 0.544300 2.2:-55193104 MS.gene058266:CDS
GTTGTCACAACAACAGTATG+TGG 0.552341 2.2:-55192156 MS.gene058266:CDS
ACTGGCTCACCATCAACCCT+AGG 0.552430 2.2:+55193064 None:intergenic
GAGATCTTGATGAAGACCCT+AGG 0.554127 2.2:-55193081 MS.gene058266:CDS
TATCACAGATGAAGGGGAGC+CGG 0.555230 2.2:-55192918 MS.gene058266:CDS
CCTGTAAGAGTTGCCACAGA+AGG 0.558919 2.2:-55193106 MS.gene058266:CDS
GGACTCCACAAGAAATATTG+AGG 0.565714 2.2:-55191066 MS.gene058266:CDS
ACTCTTACAGGAGGAGTGAT+CGG 0.576759 2.2:+55193118 None:intergenic
TTCTACCCTAAGGGAGCAGA+TGG 0.580686 2.2:-55191004 MS.gene058266:CDS
AGTGGTAATGAGAGTGTCCC+CGG 0.583706 2.2:-55191031 MS.gene058266:CDS
CTAGAAAGATTCAACATGAA+GGG 0.587088 2.2:-55192125 MS.gene058266:CDS
CACAACAACAGTATGTGGAA+CGG 0.590500 2.2:-55192151 MS.gene058266:CDS
CCAGAAACACCAAAATGTGA+TGG 0.595828 2.2:-55193157 MS.gene058266:CDS
ACATCTGTATCACAGATGAA+GGG 0.596386 2.2:-55192925 MS.gene058266:CDS
GTACTCCGAACTAGGGTACC+TGG 0.597846 2.2:+55192946 None:intergenic
AGATCTTGATGAAGACCCTA+GGG 0.607768 2.2:-55193080 MS.gene058266:CDS
TACAGATGTACTCCGAACTA+GGG 0.619016 2.2:+55192939 None:intergenic
GTTGATAGTGGGACAAGACA+AGG 0.620065 2.2:-55192297 MS.gene058266:intron
CATCTGTATCACAGATGAAG+GGG 0.621190 2.2:-55192924 MS.gene058266:CDS
GATGATGAAGATGTTCAGGA+TGG 0.628053 2.2:-55193025 MS.gene058266:CDS
GGTGAGCCAGTAATTGAAGA+TGG 0.629717 2.2:-55193052 MS.gene058266:CDS
GTAAGAGTTGCCACAGAAGG+GGG 0.631031 2.2:-55193103 MS.gene058266:CDS
CTGGCTCACCATCAACCCTA+GGG 0.637088 2.2:+55193065 None:intergenic
CATCAAACTCCCAACTGCTG+AGG 0.637421 2.2:+55191998 None:intergenic
GATATCGTCAACCATCTACC+AGG 0.639657 2.2:-55192964 MS.gene058266:CDS
TCTGTGGCAACTCTTACAGG+AGG 0.691602 2.2:+55193109 None:intergenic
GGTAATGAGAGTGTCCCCGG+TGG 0.752844 2.2:-55191028 MS.gene058266:CDS
CCTTAGGGTAGAAGCCACCG+GGG 0.757955 2.2:+55191014 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATATTGAGGATTATATA+TGG - chr2.2:55193090-55193109 MS.gene058266:CDS 20.0%
!!! AATTGATTTTTTCTTCCTTT+TGG + chr2.2:55192893-55192912 None:intergenic 20.0%
!!! AATTTGTTTTTTCTTCCTTT+TGG - chr2.2:55192966-55192985 MS.gene058266:CDS 20.0%
! AAAAAGATTTCTTGATCAAC+TGG - chr2.2:55192523-55192542 MS.gene058266:intron 25.0%
! AAAAGATTTCTTGATCAACT+GGG - chr2.2:55192524-55192543 MS.gene058266:intron 25.0%
! AAATTTCTTATACCATTGTC+TGG + chr2.2:55191715-55191734 None:intergenic 25.0%
! AATTTCTTATACCATTGTCT+GGG + chr2.2:55191714-55191733 None:intergenic 25.0%
! ATAATCCTCAATATTTCTTG+TGG + chr2.2:55193087-55193106 None:intergenic 25.0%
! ATAATTCACTATCATATCCA+AGG - chr2.2:55193058-55193077 MS.gene058266:CDS 25.0%
! ATATTGATGTCAGATATCAT+TGG - chr2.2:55192636-55192655 MS.gene058266:intron 25.0%
! TATCATCTACATATAACAGA+AGG + chr2.2:55191827-55191846 None:intergenic 25.0%
! TTTAGATGAAGAAATCTACA+TGG - chr2.2:55191605-55191624 MS.gene058266:intron 25.0%
!!! ATAAGTATCATTTTTCTGCA+AGG + chr2.2:55191325-55191344 None:intergenic 25.0%
!!! ATGTTTTGACATATACACAA+TGG + chr2.2:55191782-55191801 None:intergenic 25.0%
AAATTGAAGAAGAGCTTGTA+CGG - chr2.2:55191675-55191694 MS.gene058266:intron 30.0%
ATTGAACTCATCTTAACAAC+TGG + chr2.2:55191504-55191523 None:intergenic 30.0%
CTAGAAAGATTCAACATGAA+GGG - chr2.2:55192020-55192039 MS.gene058266:CDS 30.0%
CTCATAAGGATAAAATCCAA+TGG - chr2.2:55191271-55191290 MS.gene058266:intron 30.0%
GGAAGAACAACAATTTATGT+TGG - chr2.2:55192673-55192692 MS.gene058266:intron 30.0%
GTATATGTCAAAACATTTCG+AGG - chr2.2:55191786-55191805 MS.gene058266:intron 30.0%
GTGAATTATTATCAGCAGAA+AGG + chr2.2:55193047-55193066 None:intergenic 30.0%
TCAAGATGAAAAAGAAGCTA+TGG - chr2.2:55192109-55192128 MS.gene058266:CDS 30.0%
TCCTTTGACATGAAAAATCT+AGG - chr2.2:55191906-55191925 MS.gene058266:intron 30.0%
TCTAGAAAGATTCAACATGA+AGG - chr2.2:55192019-55192038 MS.gene058266:CDS 30.0%
TTGAGGATTATATATGGAAG+TGG - chr2.2:55193096-55193115 MS.gene058266:CDS 30.0%
TTTCTTCATCTAAATCTCCA+TGG + chr2.2:55191599-55191618 None:intergenic 30.0%
! AGTTCAATTCGAGTTATACT+CGG - chr2.2:55191516-55191535 MS.gene058266:intron 30.0%
! TATGATTGGTGATTTGAAGA+AGG - chr2.2:55191872-55191891 MS.gene058266:intron 30.0%
! TGTAGATGATATGTTGATAG+TGG - chr2.2:55191836-55191855 MS.gene058266:intron 30.0%
!! GTAGATGATATGTTGATAGT+GGG - chr2.2:55191837-55191856 MS.gene058266:intron 30.0%
!! TCTTTTTCATCTTGACTAGT+AGG + chr2.2:55192104-55192123 None:intergenic 30.0%
AAAGCTCGCTTAGTTGTTAA+AGG - chr2.2:55191435-55191454 MS.gene058266:intron 35.0%
AACACCAAAATGTGATGGTA+AGG - chr2.2:55190993-55191012 MS.gene058266:CDS 35.0%
AATATTTCTTGTGGAGTCCT+TGG + chr2.2:55193078-55193097 None:intergenic 35.0%
AATGGTGTTTACTTTCTCAG+GGG - chr2.2:55192397-55192416 MS.gene058266:intron 35.0%
ACATCTGTATCACAGATGAA+GGG - chr2.2:55191220-55191239 MS.gene058266:intron 35.0%
CAATCCTGGTAAAGAACATT+GGG - chr2.2:55192235-55192254 MS.gene058266:CDS 35.0%
CGATGAAGTTCAAACTCATA+AGG - chr2.2:55191257-55191276 MS.gene058266:intron 35.0%
GAATGGTGTTTACTTTCTCA+GGG - chr2.2:55192396-55192415 MS.gene058266:intron 35.0%
GGATAAAATCCAATGGATGA+AGG - chr2.2:55191278-55191297 MS.gene058266:intron 35.0%
TACATCTGTATCACAGATGA+AGG - chr2.2:55191219-55191238 MS.gene058266:intron 35.0%
TAGAAAGTCTACTTCAGGAA+TGG - chr2.2:55192379-55192398 MS.gene058266:intron 35.0%
TCACACTGATAAAAATCCTG+CGG - chr2.2:55192703-55192722 MS.gene058266:intron 35.0%
TCACTACCATCTTCAATTAC+TGG + chr2.2:55191102-55191121 None:intergenic 35.0%
TCTGATAAGTGCTTATGCTT+TGG - chr2.2:55192296-55192315 MS.gene058266:intron 35.0%
TGAACTTCATCGAAACTTTC+CGG + chr2.2:55191249-55191268 None:intergenic 35.0%
! AATTCGAGTTATACTCGGAT+TGG - chr2.2:55191521-55191540 MS.gene058266:intron 35.0%
! AGATTTGGAGCTTGAACAAA+TGG - chr2.2:55191554-55191573 MS.gene058266:intron 35.0%
! ATACCTAGAATTTGCTTTGC+AGG + chr2.2:55191933-55191952 None:intergenic 35.0%
! ATTGGTTGCTAGCTTAGATT+TGG - chr2.2:55191539-55191558 MS.gene058266:intron 35.0%
! GCCTAGATTTTTCATGTCAA+AGG + chr2.2:55191910-55191929 None:intergenic 35.0%
! TACCTAGAATTTGCTTTGCA+GGG + chr2.2:55191932-55191951 None:intergenic 35.0%
! TAGTGATGATGAAGATGTTC+AGG - chr2.2:55191116-55191135 MS.gene058266:intron 35.0%
! TCAACCTTACCATCACATTT+TGG + chr2.2:55191000-55191019 None:intergenic 35.0%
! TCTGCTGAGTTTAAAGTGAT+TGG + chr2.2:55192072-55192091 None:intergenic 35.0%
!! GGATCAGAATCGATTTTGAA+AGG - chr2.2:55192320-55192339 MS.gene058266:intron 35.0%
!!! GATTTTTTCTTCCTTTTGGC+TGG + chr2.2:55192889-55192908 None:intergenic 35.0%
!!! TGTTTTTTCTTCCTTTTGGC+TGG - chr2.2:55192970-55192989 MS.gene058266:CDS 35.0%
AAAAGCCCTGAAGAACAAGT+GGG - chr2.2:55191371-55191390 MS.gene058266:intron 40.0%
AAGTAGACTTTCTACCATCC+AGG + chr2.2:55192373-55192392 None:intergenic 40.0%
ACAACAACAGTATGTGGAAC+GGG - chr2.2:55191995-55192014 MS.gene058266:intron 40.0%
AGAGGCAGAATATATTGCCA+TGG - chr2.2:55192472-55192491 MS.gene058266:intron 40.0%
AGATCTTGATGAAGACCCTA+GGG - chr2.2:55191065-55191084 MS.gene058266:CDS 40.0%
AGGATTTGAGAAGACTCGAA+AGG - chr2.2:55191638-55191657 MS.gene058266:intron 40.0%
ATACAGATGTACTCCGAACT+AGG + chr2.2:55191210-55191229 None:intergenic 40.0%
ATCTACATGGTTCAACCAGA+AGG - chr2.2:55191618-55191637 MS.gene058266:intron 40.0%
ATGGTGTTTACTTTCTCAGG+GGG - chr2.2:55192398-55192417 MS.gene058266:intron 40.0%
CAACTTTGTGCCGAATTGAT+CGG - chr2.2:55192761-55192780 MS.gene058266:intron 40.0%
CAATATGCTTTGTACGAGAG+TGG + chr2.2:55192622-55192641 None:intergenic 40.0%
CACAACAACAGTATGTGGAA+CGG - chr2.2:55191994-55192013 MS.gene058266:intron 40.0%
CATCTGTATCACAGATGAAG+GGG - chr2.2:55191221-55191240 MS.gene058266:intron 40.0%
CCAATCCTGGTAAAGAACAT+TGG - chr2.2:55192234-55192253 MS.gene058266:CDS 40.0%
CCAATGTTCTTTACCAGGAT+TGG + chr2.2:55192237-55192256 None:intergenic 40.0%
CCAGAAACACCAAAATGTGA+TGG - chr2.2:55190988-55191007 MS.gene058266:CDS 40.0%
CGCTTAGTTGTTAAAGGCTT+TGG - chr2.2:55191441-55191460 MS.gene058266:intron 40.0%
GATAAGTGCTTATGCTTTGG+AGG - chr2.2:55192299-55192318 MS.gene058266:intron 40.0%
GATGATGAAGATGTTCAGGA+TGG - chr2.2:55191120-55191139 MS.gene058266:intron 40.0%
GATGGTAGAAAGTCTACTTC+AGG - chr2.2:55192374-55192393 MS.gene058266:intron 40.0%
GGAATGGTGTTTACTTTCTC+AGG - chr2.2:55192395-55192414 MS.gene058266:intron 40.0%
GGACAAGACAAGGATATGAT+TGG - chr2.2:55191858-55191877 MS.gene058266:intron 40.0%
GGACTCCACAAGAAATATTG+AGG - chr2.2:55193079-55193098 MS.gene058266:CDS 40.0%
GTAAAGACTGCATTTCTCCA+TGG - chr2.2:55191579-55191598 MS.gene058266:intron 40.0%
GTGTTCAAGCTCAAGAAAGA+TGG - chr2.2:55191393-55191412 MS.gene058266:intron 40.0%
GTTGTCACAACAACAGTATG+TGG - chr2.2:55191989-55192008 MS.gene058266:intron 40.0%
TACAGATGTACTCCGAACTA+GGG + chr2.2:55191209-55191228 None:intergenic 40.0%
TGGGAGTTTGATGTATGCAA+TGG - chr2.2:55192157-55192176 MS.gene058266:CDS 40.0%
TGTTGCCTTATCAACAACAG+AGG - chr2.2:55192454-55192473 MS.gene058266:intron 40.0%
TTCTGCCTCTGTTGTTGATA+AGG + chr2.2:55192462-55192481 None:intergenic 40.0%
! AGCAGAAAGGATGTTTCTGT+TGG + chr2.2:55193034-55193053 None:intergenic 40.0%
! GTATGAAGATTTTGCGAGAC+CGG - chr2.2:55191949-55191968 MS.gene058266:intron 40.0%
! TGGATCTTGAGGTATCTGAA+AGG - chr2.2:55192269-55192288 MS.gene058266:CDS 40.0%
!!! CCATCACATTTTGGTGTTTC+TGG + chr2.2:55190991-55191010 None:intergenic 40.0%
AACATTGGGAAGCTGTGAAG+TGG - chr2.2:55192249-55192268 MS.gene058266:CDS 45.0%
AAGCAGGGAAGGAAATGCTT+TGG - chr2.2:55192498-55192517 MS.gene058266:intron 45.0%
AAGCGCTTTGACTGTCACAA+TGG + chr2.2:55192574-55192593 None:intergenic 45.0%
AAGCTGTGAAGTGGATCTTG+AGG - chr2.2:55192258-55192277 MS.gene058266:CDS 45.0%
ACAATGGTCTGCATCTGTCT+TGG + chr2.2:55191766-55191785 None:intergenic 45.0%
ACCCACGTGAAAAGCAAAGA+AGG - chr2.2:55192936-55192955 MS.gene058266:CDS 45.0%
ACTCTTACAGGAGGAGTGAT+CGG + chr2.2:55191030-55191049 None:intergenic 45.0%
ACTTTCGTCAACATATCCGC+AGG + chr2.2:55192722-55192741 None:intergenic 45.0%
AGCAGGTTTCTTTCCAATCC+TGG - chr2.2:55192221-55192240 MS.gene058266:CDS 45.0%
AGGCTACACAGATGCTGATA+TGG - chr2.2:55192340-55192359 MS.gene058266:intron 45.0%
ATATTGCCATGGCTGAAGCA+GGG - chr2.2:55192483-55192502 MS.gene058266:intron 45.0%
ATCTACCAGGTACCCTAGTT+CGG - chr2.2:55191194-55191213 MS.gene058266:intron 45.0%
ATCTCCTGAGCTGAGATGTT+GGG + chr2.2:55191155-55191174 None:intergenic 45.0%
ATGCAGTTGGAGTTGTTAGC+AGG - chr2.2:55192204-55192223 MS.gene058266:CDS 45.0%
ATTGCGAGGATATCTCCCTT+TGG - chr2.2:55192804-55192823 MS.gene058266:intron 45.0%
CCAACTGCATGAGCAATATC+TGG + chr2.2:55192194-55192213 None:intergenic 45.0%
CCAGATATTGCTCATGCAGT+TGG - chr2.2:55192191-55192210 MS.gene058266:CDS 45.0%
CGAGTCTTCTCAAATCCTTC+TGG + chr2.2:55191636-55191655 None:intergenic 45.0%
CTGTAAGAGTTGCCACAGAA+GGG - chr2.2:55191040-55191059 MS.gene058266:CDS 45.0%
CTTGCATTGCCTTCATCCAT+TGG + chr2.2:55191290-55191309 None:intergenic 45.0%
GAAAAGCCCTGAAGAACAAG+TGG - chr2.2:55191370-55191389 MS.gene058266:intron 45.0%
GAACACCCACTTGTTCTTCA+GGG + chr2.2:55191379-55191398 None:intergenic 45.0%
GAAGGCAATGCAAGAAGAGA+TGG - chr2.2:55191296-55191315 MS.gene058266:intron 45.0%
GAGATCTTGATGAAGACCCT+AGG - chr2.2:55191064-55191083 MS.gene058266:CDS 45.0%
GAGCTTGTGAAACTTCCTGA+AGG - chr2.2:55191345-55191364 MS.gene058266:intron 45.0%
GATATCGTCAACCATCTACC+AGG - chr2.2:55191181-55191200 MS.gene058266:intron 45.0%
GCTTCCCAATGTTCTTTACC+AGG + chr2.2:55192242-55192261 None:intergenic 45.0%
GGTGAGCCAGTAATTGAAGA+TGG - chr2.2:55191093-55191112 MS.gene058266:intron 45.0%
TACACAGATGCTGATATGGC+AGG - chr2.2:55192344-55192363 MS.gene058266:intron 45.0%
TACCTTACTCCTCAGCAGTT+GGG - chr2.2:55192138-55192157 MS.gene058266:CDS 45.0%
TATATTGCCATGGCTGAAGC+AGG - chr2.2:55192482-55192501 MS.gene058266:intron 45.0%
TCGCAAGTGAATGAATTGCG+AGG - chr2.2:55192790-55192809 MS.gene058266:intron 45.0%
TGAAACAAGCTCCCAGACAA+TGG - chr2.2:55191700-55191719 MS.gene058266:intron 45.0%
TGAACACCCACTTGTTCTTC+AGG + chr2.2:55191380-55191399 None:intergenic 45.0%
TGATCAACTGGGAATGAAGC+AGG - chr2.2:55192535-55192554 MS.gene058266:intron 45.0%
TGTAAGAGTTGCCACAGAAG+GGG - chr2.2:55191041-55191060 MS.gene058266:CDS 45.0%
! ACCATAGCTTCCTAGCTTTC+CGG + chr2.2:55191971-55191990 None:intergenic 45.0%
! TTGTGACAGTCAAAGCGCTT+TGG - chr2.2:55192574-55192593 MS.gene058266:intron 45.0%
!! AAAGGCTTTGGACAAAAGCG+AGG - chr2.2:55191453-55191472 MS.gene058266:intron 45.0%
!! CAACATGAAGGGTGCTAAAC+CGG - chr2.2:55192031-55192050 MS.gene058266:CDS 45.0%
!! GCCTTCTTTGCTTTTCACGT+GGG + chr2.2:55192940-55192959 None:intergenic 45.0%
!! GTTGATAGTGGGACAAGACA+AGG - chr2.2:55191848-55191867 MS.gene058266:intron 45.0%
!! TGCCTTCTTTGCTTTTCACG+TGG + chr2.2:55192941-55192960 None:intergenic 45.0%
!! TTCTTTGCTTTTCACGTGGG+TGG + chr2.2:55192937-55192956 None:intergenic 45.0%
AATCCCCAACATCTCAGCTC+AGG - chr2.2:55191148-55191167 MS.gene058266:intron 50.0%
ACCGGAAAGCTAGGAAGCTA+TGG - chr2.2:55191967-55191986 MS.gene058266:intron 50.0%
ATACGACCATCTGCTCCCTT+AGG + chr2.2:55193150-55193169 None:intergenic 50.0%
ATATCTCCCTTTGGCTGGAG+GGG - chr2.2:55192813-55192832 MS.gene058266:intron 50.0%
ATCAAGATCTCCCCCTTCTG+TGG + chr2.2:55191055-55191074 None:intergenic 50.0%
CATCAAACTCCCAACTGCTG+AGG + chr2.2:55192150-55192169 None:intergenic 50.0%
CCTGTAAGAGTTGCCACAGA+AGG - chr2.2:55191039-55191058 MS.gene058266:CDS 50.0%
CCTTCTGTGGCAACTCTTAC+AGG + chr2.2:55191042-55191061 None:intergenic 50.0%
GATATCTCCCTTTGGCTGGA+GGG - chr2.2:55192812-55192831 MS.gene058266:intron 50.0%
GATCTCCTGAGCTGAGATGT+TGG + chr2.2:55191156-55191175 None:intergenic 50.0%
GATGAAGACCCTAGGGTTGA+TGG - chr2.2:55191072-55191091 MS.gene058266:intron 50.0%
GCGATCCATGCCGATCAATT+CGG + chr2.2:55192774-55192793 None:intergenic 50.0%
GGCCCTGCAAAGCAAATTCT+AGG - chr2.2:55191927-55191946 MS.gene058266:intron 50.0%
GTAAGAGTTGCCACAGAAGG+GGG - chr2.2:55191042-55191061 MS.gene058266:CDS 50.0%
GTACCTTACTCCTCAGCAGT+TGG - chr2.2:55192137-55192156 MS.gene058266:CDS 50.0%
TACGACCATCTGCTCCCTTA+GGG + chr2.2:55193149-55193168 None:intergenic 50.0%
TATCACAGATGAAGGGGAGC+CGG - chr2.2:55191227-55191246 MS.gene058266:intron 50.0%
TCTCCTGAGCTGAGATGTTG+GGG + chr2.2:55191154-55191173 None:intergenic 50.0%
TCTGTGGCAACTCTTACAGG+AGG + chr2.2:55191039-55191058 None:intergenic 50.0%
TGTACCACCAACCCACTCAT+GGG - chr2.2:55192999-55193018 MS.gene058266:CDS 50.0%
TGTACCACCAACCCACTCAT+GGG + chr2.2:55193018-55192999 None:intergenic 50.0%
TTCTACCCTAAGGGAGCAGA+TGG - chr2.2:55193141-55193160 MS.gene058266:CDS 50.0%
TTGGCTAACGACGTGCTAAC+CGG + chr2.2:55192053-55192072 None:intergenic 50.0%
TTGTGCCGAATTGATCGGCA+TGG - chr2.2:55192766-55192785 MS.gene058266:intron 50.0%
! GAACTAGGGTACCTGGTAGA+TGG + chr2.2:55191195-55191214 None:intergenic 50.0%
! GTACAGAGCATCCAGCCAAA+AGG - chr2.2:55192875-55192894 MS.gene058266:intron 50.0%
! GTACAGAGCATCCAGCCAAA+AGG + chr2.2:55192894-55192875 None:intergenic 50.0%
! TCATTGGTTGCGTCAAGCAG+CGG - chr2.2:55192652-55192671 MS.gene058266:intron 50.0%
! TTCAGGGCTTTTCTGCCTTC+AGG + chr2.2:55191363-55191382 None:intergenic 50.0%
! TTTTGCGAGACCGGAAAGCT+AGG - chr2.2:55191958-55191977 MS.gene058266:intron 50.0%
!! AGTGGTAATGAGAGTGTCCC+CGG - chr2.2:55193114-55193133 MS.gene058266:CDS 50.0%
!! GAAGACTCGAAAGGAGCACT+TGG - chr2.2:55191647-55191666 MS.gene058266:intron 50.0%
AACCCACTCATGGGCAAGTG+GGG - chr2.2:55193008-55193027 MS.gene058266:CDS 55.0%
AACCCACTCATGGGCAAGTG+GGG + chr2.2:55193027-55193008 None:intergenic 55.0%
AATTCTCCCCCTCCAGCCAA+AGG + chr2.2:55192823-55192842 None:intergenic 55.0%
ATTCTCCCCCTCCAGCCAAA+GGG + chr2.2:55192822-55192841 None:intergenic 55.0%
CAACCCACTCATGGGCAAGT+GGG - chr2.2:55193007-55193026 MS.gene058266:CDS 55.0%
CAACCCACTCATGGGCAAGT+GGG + chr2.2:55193026-55193007 None:intergenic 55.0%
CGAGGATATCTCCCTTTGGC+TGG - chr2.2:55192808-55192827 MS.gene058266:intron 55.0%
CTCCCAACTGCTGAGGAGTA+AGG + chr2.2:55192143-55192162 None:intergenic 55.0%
CTGGCTCACCATCAACCCTA+GGG + chr2.2:55191083-55191102 None:intergenic 55.0%
CTGTACCACCAACCCACTCA+TGG - chr2.2:55192998-55193017 MS.gene058266:CDS 55.0%
CTGTACCACCAACCCACTCA+TGG + chr2.2:55193017-55192998 None:intergenic 55.0%
GATATGGCAGGTGACCTGGA+TGG - chr2.2:55192356-55192375 MS.gene058266:intron 55.0%
GGATATCTCCCTTTGGCTGG+AGG - chr2.2:55192811-55192830 MS.gene058266:intron 55.0%
TATCTCCCTTTGGCTGGAGG+GGG - chr2.2:55192814-55192833 MS.gene058266:intron 55.0%
TCCCTTAGGGTAGAAGCCAC+CGG + chr2.2:55193136-55193155 None:intergenic 55.0%
TCTCAGGGGGAGCTGTATCA+TGG - chr2.2:55192411-55192430 MS.gene058266:intron 55.0%
TGCTGATATGGCAGGTGACC+TGG - chr2.2:55192352-55192371 MS.gene058266:intron 55.0%
TTCCTTCCCTGCTTCAGCCA+TGG + chr2.2:55192492-55192511 None:intergenic 55.0%
! ACTGGCTCACCATCAACCCT+AGG + chr2.2:55191084-55191103 None:intergenic 55.0%
! GTACTCCGAACTAGGGTACC+TGG + chr2.2:55191202-55191221 None:intergenic 55.0%
CCAACCCACTCATGGGCAAG+TGG - chr2.2:55193006-55193025 MS.gene058266:CDS 60.0%
CCAACCCACTCATGGGCAAG+TGG + chr2.2:55193025-55193006 None:intergenic 60.0%
CCACTTGCCCATGAGTGGGT+TGG - chr2.2:55192850-55192869 MS.gene058266:intron 60.0%
CCACTTGCCCATGAGTGGGT+TGG + chr2.2:55192869-55192850 None:intergenic 60.0%
CCCGGTGGCTTCTACCCTAA+GGG - chr2.2:55193132-55193151 MS.gene058266:CDS 60.0%
CCCTTAGGGTAGAAGCCACC+GGG + chr2.2:55193135-55193154 None:intergenic 60.0%
CCTTAGGGTAGAAGCCACCG+GGG + chr2.2:55193134-55193153 None:intergenic 60.0%
GGCTGGAGGGGGAGAATTGT+TGG - chr2.2:55192825-55192844 MS.gene058266:intron 60.0%
TGCCATGGCTGAAGCAGGGA+AGG - chr2.2:55192487-55192506 MS.gene058266:intron 60.0%
!! CTTGCCCATGAGTGGGTTGG+TGG - chr2.2:55192853-55192872 MS.gene058266:intron 60.0%
!! CTTGCCCATGAGTGGGTTGG+TGG + chr2.2:55192872-55192853 None:intergenic 60.0%
!! GGTAATGAGAGTGTCCCCGG+TGG - chr2.2:55193117-55193136 MS.gene058266:CDS 60.0%
CCCCGGTGGCTTCTACCCTA+AGG - chr2.2:55193131-55193150 MS.gene058266:CDS 65.0%
GGCCCCACTTGCCCATGAGT+GGG - chr2.2:55192846-55192865 MS.gene058266:intron 65.0%
GGCCCCACTTGCCCATGAGT+GGG + chr2.2:55192865-55192846 None:intergenic 65.0%
TGGCCCCACTTGCCCATGAG+TGG - chr2.2:55192845-55192864 MS.gene058266:intron 65.0%
TGGCCCCACTTGCCCATGAG+TGG + chr2.2:55192864-55192845 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 55190970 55193197 55190970 ID=MS.gene058266
chr2.2 mRNA 55190970 55193197 55190970 ID=MS.gene058266.t1;Parent=MS.gene058266
chr2.2 exon 55192915 55193197 55192915 ID=MS.gene058266.t1.exon1;Parent=MS.gene058266.t1
chr2.2 CDS 55192915 55193197 55192915 ID=cds.MS.gene058266.t1;Parent=MS.gene058266.t1
chr2.2 exon 55191999 55192311 55191999 ID=MS.gene058266.t1.exon2;Parent=MS.gene058266.t1
chr2.2 CDS 55191999 55192311 55191999 ID=cds.MS.gene058266.t1;Parent=MS.gene058266.t1
chr2.2 exon 55190970 55191087 55190970 ID=MS.gene058266.t1.exon3;Parent=MS.gene058266.t1
chr2.2 CDS 55190970 55191087 55190970 ID=cds.MS.gene058266.t1;Parent=MS.gene058266.t1
Gene Sequence

>MS.gene058266

ATGTCAGATTCTGCTATTCCAGAAACACCAAAATGTGATGGTAAGGTTGATCTTACACCGATCACTCCTCCTGTAAGAGTTGCCACAGAAGGGGGAGATCTTGATGAAGACCCTAGGGTTGATGGTGAGCCAGTAATTGAAGATGGTAGTGATGATGAAGATGTTCAGGATGGAGAGCAATCCCCAACATCTCAGCTCAGGAGATCAAGCAGATATCGTCAACCATCTACCAGGTACCCTAGTTCGGAGTACATCTGTATCACAGATGAAGGGGAGCCGGAAAGTTTCGATGAAGTTCAAACTCATAAGGATAAAATCCAATGGATGAAGGCAATGCAAGAAGAGATGGATTCCTTGCAGAAAAATGATACTTATGAGCTTGTGAAACTTCCTGAAGGCAGAAAAGCCCTGAAGAACAAGTGGGTGTTCAAGCTCAAGAAAGATGGTGACAAGCTAGTGAAGTACAAAGCTCGCTTAGTTGTTAAAGGCTTTGGACAAAAGCGAGGAATAGATTTTGATGAAATCTTCTCTCCAGTTGTTAAGATGAGTTCAATTCGAGTTATACTCGGATTGGTTGCTAGCTTAGATTTGGAGCTTGAACAAATGGATGTAAAGACTGCATTTCTCCATGGAGATTTAGATGAAGAAATCTACATGGTTCAACCAGAAGGATTTGAGAAGACTCGAAAGGAGCACTTGGTTTGCAAATTGAAGAAGAGCTTGTACGGATTGAAACAAGCTCCCAGACAATGGTATAAGAAATTTGACTCTTTCATGATGAGTCATGAATATACCAAGACAGATGCAGACCATTGTGTATATGTCAAAACATTTCGAGGTGACAAATTCATCATCCTTCTGTTATATGTAGATGATATGTTGATAGTGGGACAAGACAAGGATATGATTGGTGATTTGAAGAAGGAATTGTCAAAATCCTTTGACATGAAAAATCTAGGCCCTGCAAAGCAAATTCTAGGTATGAAGATTTTGCGAGACCGGAAAGCTAGGAAGCTATGGTTGTCACAACAACAGTATGTGGAACGGGTTCTAGAAAGATTCAACATGAAGGGTGCTAAACCGGTTAGCACGTCGTTAGCCAATCACTTTAAACTCAGCAGAGTGAGTTGTCCTACTAGTCAAGATGAAAAAGAAGCTATGGCTGCAGTACCTTACTCCTCAGCAGTTGGGAGTTTGATGTATGCAATGGTTTGCACTAGACCAGATATTGCTCATGCAGTTGGAGTTGTTAGCAGGTTTCTTTCCAATCCTGGTAAAGAACATTGGGAAGCTGTGAAGTGGATCTTGAGGTATCTGAAAGGAAGCTCTGATAAGTGCTTATGCTTTGGAGGATCAGAATCGATTTTGAAAGGCTACACAGATGCTGATATGGCAGGTGACCTGGATGGTAGAAAGTCTACTTCAGGAATGGTGTTTACTTTCTCAGGGGGAGCTGTATCATGGCAATCTAAATTGCAAAAATGTGTTGCCTTATCAACAACAGAGGCAGAATATATTGCCATGGCTGAAGCAGGGAAGGAAATGCTTTGGCTAAAAAGATTTCTTGATCAACTGGGAATGAAGCAGGAAAAGTATGTTATCCATTGTGACAGTCAAAGCGCTTTGGATTTGAGCAAGAATGCGATGTACCACTCTCGTACAAAGCATATTGATGTCAGATATCATTGGTTGCGTCAAGCAGCGGAAGAACAACAATTTATGTTGGAAAAGATTCACACTGATAAAAATCCTGCGGATATGTTGACGAAAGTTGTAGCACGAGAAAAGCTTCAACTTTGTGCCGAATTGATCGGCATGGATCGCAAGTGAATGAATTGCGAGGATATCTCCCTTTGGCTGGAGGGGGAGAATTGTTGGCCCCACTTGCCCATGAGTGGGTTGGTGGTACAGAGCATCCAGCCAAAAGGAAGAAAAAATCAATTGAAACACAATGTTGAATTCTTCCACCCACGTGAAAAGCAAAGAAGGCAAAATCAATTTGTTTTTTCTTCCTTTTGGCTGGATGCTCTGTACCACCAACCCACTCATGGGCAAGTGGGGCCAACAGAAACATCCTTTCTGCTGATAATAATTCACTATCATATCCAAGGACTCCACAAGAAATATTGAGGATTATATATGGAAGTGGTAATGAGAGTGTCCCCGGTGGCTTCTACCCTAAGGGAGCAGATGGTCGTATAGCAAGATATTACTTACACAGTGCTTAA
Protein sequence

>MS.gene058266.t1

MSDSAIPETPKCDGKVDLTPITPPVRVATEGGDLDEDPRVDGEPVIEDGSDDEDVQDGEQSPTSQLRRSSRYRQPSTRYPSSEYICITDEGEPEMGQDKDMIGDLKKELSKSFDMKNLGPAKQILGMKILRDRKARKLWLSQQQYVERVLERFNMKGAKPVSTSLANHFKLSRVSCPTSQDEKEAMAAVPYSSAVGSLMTPQEILRIIYGSGNESVPGGFYPKGADGRIARYYLHSA