Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48478.t1 | XP_003603001.1 | 100 | 237 | 0 | 0 | 1 | 237 | 1 | 237 | 5.60E-131 | 476.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48478.t1 | O22227 | 44.8 | 172 | 82 | 4 | 72 | 235 | 130 | 296 | 3.5e-33 | 143.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48478.t1 | G7J5T6 | 100.0 | 237 | 0 | 0 | 1 | 237 | 1 | 237 | 4.0e-131 | 476.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053074 | MS.gene48478 | 0.80543 | 1.34E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48478.t1 | MTR_3g101300 | 100.000 | 237 | 0 | 0 | 1 | 237 | 1 | 237 | 0.0 | 496 |
| MS.gene48478.t1 | MTR_8g040690 | 41.975 | 243 | 119 | 9 | 2 | 236 | 19 | 247 | 2.63e-54 | 175 |
| MS.gene48478.t1 | MTR_3g094830 | 45.098 | 204 | 96 | 7 | 41 | 235 | 62 | 258 | 6.56e-52 | 169 |
| MS.gene48478.t1 | MTR_8g040680 | 43.689 | 206 | 103 | 6 | 36 | 236 | 60 | 257 | 2.12e-51 | 167 |
| MS.gene48478.t1 | MTR_2g054380 | 49.102 | 167 | 76 | 3 | 72 | 235 | 109 | 269 | 3.77e-48 | 160 |
| MS.gene48478.t1 | MTR_4g112370 | 46.821 | 173 | 77 | 6 | 69 | 235 | 96 | 259 | 2.02e-44 | 150 |
| MS.gene48478.t1 | MTR_2g055990 | 45.000 | 180 | 87 | 6 | 60 | 235 | 87 | 258 | 5.06e-43 | 146 |
| MS.gene48478.t1 | MTR_1g053130 | 45.665 | 173 | 79 | 4 | 72 | 235 | 117 | 283 | 2.42e-39 | 137 |
| MS.gene48478.t1 | MTR_3g086740 | 42.458 | 179 | 86 | 7 | 65 | 236 | 75 | 243 | 7.00e-38 | 132 |
| MS.gene48478.t1 | MTR_7g064810 | 41.667 | 180 | 87 | 6 | 60 | 236 | 59 | 223 | 2.99e-35 | 125 |
| MS.gene48478.t1 | MTR_8g089865 | 40.000 | 170 | 89 | 7 | 73 | 236 | 59 | 221 | 3.60e-33 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48478.t1 | AT5G42680 | 61.885 | 244 | 80 | 5 | 1 | 237 | 1 | 238 | 1.25e-100 | 292 |
| MS.gene48478.t1 | AT5G42680 | 61.885 | 244 | 80 | 5 | 1 | 237 | 1 | 238 | 1.25e-100 | 292 |
| MS.gene48478.t1 | AT4G39610 | 46.581 | 234 | 99 | 10 | 20 | 236 | 40 | 264 | 4.91e-58 | 185 |
| MS.gene48478.t1 | AT2G21990 | 47.291 | 203 | 92 | 7 | 41 | 236 | 58 | 252 | 1.64e-53 | 173 |
| MS.gene48478.t1 | AT5G06990 | 44.196 | 224 | 106 | 7 | 24 | 236 | 46 | 261 | 1.90e-53 | 173 |
| MS.gene48478.t1 | AT3G25640 | 50.000 | 172 | 72 | 7 | 73 | 235 | 100 | 266 | 1.14e-48 | 161 |
| MS.gene48478.t1 | AT3G25640 | 50.000 | 172 | 72 | 7 | 73 | 235 | 126 | 292 | 2.21e-48 | 161 |
| MS.gene48478.t1 | AT2G41660 | 44.509 | 173 | 83 | 4 | 71 | 235 | 129 | 296 | 2.29e-44 | 151 |
| MS.gene48478.t1 | AT5G23100 | 42.051 | 195 | 79 | 5 | 69 | 235 | 88 | 276 | 4.21e-40 | 139 |
| MS.gene48478.t1 | AT2G37880 | 43.617 | 188 | 88 | 8 | 55 | 236 | 70 | 245 | 8.43e-40 | 137 |
| MS.gene48478.t1 | AT1G21050 | 41.176 | 170 | 81 | 6 | 73 | 236 | 89 | 245 | 7.09e-36 | 127 |
| MS.gene48478.t1 | AT1G21050 | 41.176 | 170 | 81 | 6 | 73 | 236 | 125 | 281 | 1.25e-35 | 128 |
| MS.gene48478.t1 | AT5G65340 | 40.571 | 175 | 86 | 5 | 72 | 236 | 84 | 250 | 3.52e-32 | 118 |
| MS.gene48478.t1 | AT1G76610 | 38.235 | 170 | 84 | 6 | 70 | 236 | 75 | 226 | 1.19e-29 | 110 |
| MS.gene48478.t1 | AT2G22460 | 36.145 | 166 | 95 | 4 | 71 | 234 | 85 | 241 | 1.00e-25 | 101 |
Find 49 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTAGATTTGAAAGAGTAAT+TGG | 0.213989 | 3.1:-80433448 | MS.gene48478:CDS |
| TGAAAGAGTAATTGGTTCTA+AGG | 0.266032 | 3.1:-80433440 | MS.gene48478:CDS |
| GAAAAGTTGGGTATTCAATT+AGG | 0.280770 | 3.1:-80433603 | MS.gene48478:CDS |
| AGAGGTGTGTCCATGGGAGC+TGG | 0.305516 | 3.1:-80433535 | MS.gene48478:CDS |
| GTTAACTTCTGGTTGCAAAA+TGG | 0.315722 | 3.1:-80433896 | MS.gene48478:CDS |
| CTCTTCCCCATGTTAACTTC+TGG | 0.329664 | 3.1:-80433907 | MS.gene48478:CDS |
| TTGCAAAATGGTAGCACTAT+TGG | 0.330280 | 3.1:-80433884 | MS.gene48478:CDS |
| CCATTATTAATATTATTATC+AGG | 0.336954 | 3.1:+80433391 | None:intergenic |
| GCCACAACAGGAACAATCTT+TGG | 0.337368 | 3.1:-80433829 | MS.gene48478:CDS |
| CCTGATAATAATATTAATAA+TGG | 0.346292 | 3.1:-80433391 | MS.gene48478:CDS |
| TATTGTAATGGAAGAAAAGT+TGG | 0.347703 | 3.1:-80433616 | MS.gene48478:CDS |
| AGTTGTTGGAAGAGTTTGTT+TGG | 0.349468 | 3.1:-80433645 | MS.gene48478:CDS |
| TGCAAAATGGTAGCACTATT+GGG | 0.374808 | 3.1:-80433883 | MS.gene48478:CDS |
| AATGGGTGATGAAGAACTTC+AGG | 0.388037 | 3.1:-80433572 | MS.gene48478:CDS |
| TTCAATTAGGAGGAAACAAA+TGG | 0.393295 | 3.1:-80433590 | MS.gene48478:CDS |
| TACCTCTCATATAAGTCATT+TGG | 0.396969 | 3.1:+80433467 | None:intergenic |
| GTTTGGGCTGTTTATTGTAA+TGG | 0.399156 | 3.1:-80433628 | MS.gene48478:CDS |
| ACAATCTTTGGTTACAGAAA+AGG | 0.421379 | 3.1:-80433817 | MS.gene48478:CDS |
| CGAAGGAGTGATGCTATTGC+TGG | 0.423322 | 3.1:+80433994 | None:intergenic |
| ATTGTAATGGAAGAAAAGTT+GGG | 0.435261 | 3.1:-80433615 | MS.gene48478:CDS |
| ACCAACCGCATCTGATCATA+AGG | 0.447945 | 3.1:-80433506 | MS.gene48478:CDS |
| GAGGTGTGTCCATGGGAGCT+GGG | 0.448845 | 3.1:-80433534 | MS.gene48478:CDS |
| TTCAAAGCACTTGTTAGCAT+TGG | 0.451055 | 3.1:+80433733 | None:intergenic |
| TCCTTATGATCAGATGCGGT+TGG | 0.499979 | 3.1:+80433505 | None:intergenic |
| TTAAAAGATAGTGCCACAAC+AGG | 0.504621 | 3.1:-80433841 | MS.gene48478:CDS |
| AATATTAATAATGGTGCTCA+AGG | 0.504970 | 3.1:-80433382 | MS.gene48478:CDS |
| TCACTCCTTCGCCACACAAC+AGG | 0.505178 | 3.1:-80433982 | MS.gene48478:CDS |
| AGGTGTGTCCATGGGAGCTG+GGG | 0.517152 | 3.1:-80433533 | MS.gene48478:CDS |
| TGGTAACACCCCAGCTCCCA+TGG | 0.527074 | 3.1:+80433525 | None:intergenic |
| TGATATTGTTAGAATCGCAT+TGG | 0.533608 | 3.1:-80433695 | MS.gene48478:CDS |
| CGCAACAAATTCTCCGCTGG+TGG | 0.539485 | 3.1:-80433949 | MS.gene48478:CDS |
| GATCACCTGTTGTGTGGCGA+AGG | 0.553743 | 3.1:+80433977 | None:intergenic |
| CTCTAGTCCATGCTATCCAA+TGG | 0.562507 | 3.1:-80434022 | MS.gene48478:CDS |
| GATCATAAGGAAAGTTCTGA+TGG | 0.563793 | 3.1:-80433493 | MS.gene48478:CDS |
| TCAATTAGGAGGAAACAAAT+GGG | 0.565629 | 3.1:-80433589 | MS.gene48478:CDS |
| CATGGACTAGAGTGCACAGA+TGG | 0.570529 | 3.1:+80434033 | None:intergenic |
| AAGCGCAACAAATTCTCCGC+TGG | 0.575962 | 3.1:-80433952 | MS.gene48478:CDS |
| CAGGTTATGCAACATTTGAG+AGG | 0.582318 | 3.1:-80433553 | MS.gene48478:CDS |
| AAGTTGGGTATTCAATTAGG+AGG | 0.586438 | 3.1:-80433600 | MS.gene48478:CDS |
| ACATTTGAGAGGTGTGTCCA+TGG | 0.586860 | 3.1:-80433542 | MS.gene48478:CDS |
| CATTTGAGAGGTGTGTCCAT+GGG | 0.591084 | 3.1:-80433541 | MS.gene48478:CDS |
| GTTGTTGGAAGAGTTTGTTT+GGG | 0.600573 | 3.1:-80433644 | MS.gene48478:CDS |
| ACTTTCCTTATGATCAGATG+CGG | 0.609205 | 3.1:+80433501 | None:intergenic |
| TTTGCAACCAGAAGTTAACA+TGG | 0.611316 | 3.1:+80433900 | None:intergenic |
| ACCAAAGATTGTTCCTGTTG+TGG | 0.612454 | 3.1:+80433828 | None:intergenic |
| TTGCAACCAGAAGTTAACAT+GGG | 0.620058 | 3.1:+80433901 | None:intergenic |
| GACCAAATGACTTATATGAG+AGG | 0.654037 | 3.1:-80433469 | MS.gene48478:CDS |
| TGCAACCAGAAGTTAACATG+GGG | 0.660312 | 3.1:+80433902 | None:intergenic |
| CATCTTTAAGAGACCACCAG+CGG | 0.747453 | 3.1:+80433936 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CCATTATTAATATTATTATC+AGG | + | chr3.1:80433993-80434012 | None:intergenic | 15.0% |
| !! | CCTGATAATAATATTAATAA+TGG | - | chr3.1:80433990-80434009 | MS.gene48478:CDS | 15.0% |
| ! | AACGAATAAGAAAAAGTTGT+TGG | - | chr3.1:80433722-80433741 | MS.gene48478:CDS | 25.0% |
| ! | AATATTAATAATGGTGCTCA+AGG | - | chr3.1:80433999-80434018 | MS.gene48478:CDS | 25.0% |
| ! | ACAAAAAAGATACTAAACTC+AGG | + | chr3.1:80434026-80434045 | None:intergenic | 25.0% |
| !! | ATTGTAATGGAAGAAAAGTT+GGG | - | chr3.1:80433766-80433785 | MS.gene48478:CDS | 25.0% |
| !! | TATTGTAATGGAAGAAAAGT+TGG | - | chr3.1:80433765-80433784 | MS.gene48478:CDS | 25.0% |
| ACAATCTTTGGTTACAGAAA+AGG | - | chr3.1:80433564-80433583 | MS.gene48478:CDS | 30.0% | |
| GAAAAGTTGGGTATTCAATT+AGG | - | chr3.1:80433778-80433797 | MS.gene48478:CDS | 30.0% | |
| GGTAGATTTGAAAGAGTAAT+TGG | - | chr3.1:80433933-80433952 | MS.gene48478:CDS | 30.0% | |
| TACCTCTCATATAAGTCATT+TGG | + | chr3.1:80433917-80433936 | None:intergenic | 30.0% | |
| TCAATTAGGAGGAAACAAAT+GGG | - | chr3.1:80433792-80433811 | MS.gene48478:CDS | 30.0% | |
| TGATATTGTTAGAATCGCAT+TGG | - | chr3.1:80433686-80433705 | MS.gene48478:CDS | 30.0% | |
| TTCAATTAGGAGGAAACAAA+TGG | - | chr3.1:80433791-80433810 | MS.gene48478:CDS | 30.0% | |
| ! | TCTTTTAACATAGGCTTTCT+TGG | + | chr3.1:80433528-80433547 | None:intergenic | 30.0% |
| !! | TGAAAGAGTAATTGGTTCTA+AGG | - | chr3.1:80433941-80433960 | MS.gene48478:CDS | 30.0% |
| AAGTTGGGTATTCAATTAGG+AGG | - | chr3.1:80433781-80433800 | MS.gene48478:CDS | 35.0% | |
| ACTTTCCTTATGATCAGATG+CGG | + | chr3.1:80433883-80433902 | None:intergenic | 35.0% | |
| GACCAAATGACTTATATGAG+AGG | - | chr3.1:80433912-80433931 | MS.gene48478:CDS | 35.0% | |
| GGTAACTCGATGAGAAAAAT+CGG | + | chr3.1:80433630-80433649 | None:intergenic | 35.0% | |
| GTTTGGGCTGTTTATTGTAA+TGG | - | chr3.1:80433753-80433772 | MS.gene48478:CDS | 35.0% | |
| TGCAAAATGGTAGCACTATT+GGG | - | chr3.1:80433498-80433517 | MS.gene48478:CDS | 35.0% | |
| TTAAAAGATAGTGCCACAAC+AGG | - | chr3.1:80433540-80433559 | MS.gene48478:CDS | 35.0% | |
| TTGCAAAATGGTAGCACTAT+TGG | - | chr3.1:80433497-80433516 | MS.gene48478:CDS | 35.0% | |
| TTGCAACCAGAAGTTAACAT+GGG | + | chr3.1:80433483-80433502 | None:intergenic | 35.0% | |
| TTTGCAACCAGAAGTTAACA+TGG | + | chr3.1:80433484-80433503 | None:intergenic | 35.0% | |
| ! | AGTTGTTGGAAGAGTTTGTT+TGG | - | chr3.1:80433736-80433755 | MS.gene48478:CDS | 35.0% |
| ! | GATCATAAGGAAAGTTCTGA+TGG | - | chr3.1:80433888-80433907 | MS.gene48478:CDS | 35.0% |
| ! | GTGGCACTATCTTTTAACAT+AGG | + | chr3.1:80433537-80433556 | None:intergenic | 35.0% |
| ! | GTTAACTTCTGGTTGCAAAA+TGG | - | chr3.1:80433485-80433504 | MS.gene48478:CDS | 35.0% |
| ! | GTTGTTGGAAGAGTTTGTTT+GGG | - | chr3.1:80433737-80433756 | MS.gene48478:CDS | 35.0% |
| ! | TTCAAAGCACTTGTTAGCAT+TGG | + | chr3.1:80433651-80433670 | None:intergenic | 35.0% |
| AATGGGTGATGAAGAACTTC+AGG | - | chr3.1:80433809-80433828 | MS.gene48478:CDS | 40.0% | |
| ACCAAAGATTGTTCCTGTTG+TGG | + | chr3.1:80433556-80433575 | None:intergenic | 40.0% | |
| CAGGTTATGCAACATTTGAG+AGG | - | chr3.1:80433828-80433847 | MS.gene48478:CDS | 40.0% | |
| TGCAACCAGAAGTTAACATG+GGG | + | chr3.1:80433482-80433501 | None:intergenic | 40.0% | |
| ! | GCTATTGCTGGATTTTCCAT+TGG | + | chr3.1:80433378-80433397 | None:intergenic | 40.0% |
| ! | GGATTTTCCATTGGATAGCA+TGG | + | chr3.1:80433369-80433388 | None:intergenic | 40.0% |
| ACCAACCGCATCTGATCATA+AGG | - | chr3.1:80433875-80433894 | MS.gene48478:CDS | 45.0% | |
| CTCTAGTCCATGCTATCCAA+TGG | - | chr3.1:80433359-80433378 | MS.gene48478:CDS | 45.0% | |
| CTCTTCCCCATGTTAACTTC+TGG | - | chr3.1:80433474-80433493 | MS.gene48478:CDS | 45.0% | |
| GCCACAACAGGAACAATCTT+TGG | - | chr3.1:80433552-80433571 | MS.gene48478:CDS | 45.0% | |
| TCCTTATGATCAGATGCGGT+TGG | + | chr3.1:80433879-80433898 | None:intergenic | 45.0% | |
| ! | ACATTTGAGAGGTGTGTCCA+TGG | - | chr3.1:80433839-80433858 | MS.gene48478:CDS | 45.0% |
| ! | CATCTTTAAGAGACCACCAG+CGG | + | chr3.1:80433448-80433467 | None:intergenic | 45.0% |
| ! | CATTTGAGAGGTGTGTCCAT+GGG | - | chr3.1:80433840-80433859 | MS.gene48478:CDS | 45.0% |
| ! | GCTTTTGATCACCTGTTGTG+TGG | + | chr3.1:80433413-80433432 | None:intergenic | 45.0% |
| AAGCGCAACAAATTCTCCGC+TGG | - | chr3.1:80433429-80433448 | MS.gene48478:CDS | 50.0% | |
| CGAAGGAGTGATGCTATTGC+TGG | + | chr3.1:80433390-80433409 | None:intergenic | 50.0% | |
| !! | CATGGACTAGAGTGCACAGA+TGG | + | chr3.1:80433351-80433370 | None:intergenic | 50.0% |
| CGCAACAAATTCTCCGCTGG+TGG | - | chr3.1:80433432-80433451 | MS.gene48478:CDS | 55.0% | |
| GATCACCTGTTGTGTGGCGA+AGG | + | chr3.1:80433407-80433426 | None:intergenic | 55.0% | |
| TCACTCCTTCGCCACACAAC+AGG | - | chr3.1:80433399-80433418 | MS.gene48478:CDS | 55.0% | |
| AGGTGTGTCCATGGGAGCTG+GGG | - | chr3.1:80433848-80433867 | MS.gene48478:CDS | 60.0% | |
| GAGGTGTGTCCATGGGAGCT+GGG | - | chr3.1:80433847-80433866 | MS.gene48478:CDS | 60.0% | |
| TGGTAACACCCCAGCTCCCA+TGG | + | chr3.1:80433859-80433878 | None:intergenic | 60.0% | |
| ! | AGAGGTGTGTCCATGGGAGC+TGG | - | chr3.1:80433846-80433865 | MS.gene48478:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 80433345 | 80434058 | 80433345 | ID=MS.gene48478 |
| chr3.1 | mRNA | 80433345 | 80434058 | 80433345 | ID=MS.gene48478.t1;Parent=MS.gene48478 |
| chr3.1 | exon | 80433345 | 80434058 | 80433345 | ID=MS.gene48478.t1.exon1;Parent=MS.gene48478.t1 |
| chr3.1 | CDS | 80433345 | 80434058 | 80433345 | ID=cds.MS.gene48478.t1;Parent=MS.gene48478.t1 |
| Gene Sequence |
| Protein sequence |
