AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48635

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48635.t1 MTR_4g033085 95.938 320 7 1 1 320 1 314 0.0 623
MS.gene48635.t1 MTR_4g033170 95.312 320 9 1 1 320 1 314 0.0 620
MS.gene48635.t1 MTR_4g033200 77.429 319 70 2 1 318 1 318 0.0 516
MS.gene48635.t1 MTR_4g033015 77.116 319 71 2 1 318 1 318 0.0 513
MS.gene48635.t1 MTR_4g088200 76.803 319 72 1 1 319 1 317 0.0 508
MS.gene48635.t1 MTR_4g033150 75.549 319 76 2 1 318 1 318 5.39e-180 502
MS.gene48635.t1 MTR_4g088190 76.636 321 74 1 1 320 1 321 5.70e-169 474
MS.gene48635.t1 MTR_0794s0020 75.200 250 60 2 1 249 1 249 1.11e-138 393
MS.gene48635.t1 MTR_7g014570 47.799 318 156 3 6 323 6 313 1.10e-104 311
MS.gene48635.t1 MTR_7g014510 46.562 320 166 3 6 325 6 320 1.26e-102 306
MS.gene48635.t1 MTR_1g076940 48.428 318 157 5 5 319 5 318 3.58e-100 300
MS.gene48635.t1 MTR_4g088345 47.975 321 158 4 1 320 1 313 2.09e-96 290
MS.gene48635.t1 MTR_7g024500 44.514 319 165 4 6 324 5 311 1.66e-95 287
MS.gene48635.t1 MTR_7g024520 44.828 319 164 4 6 324 5 311 6.73e-94 283
MS.gene48635.t1 MTR_5g074680 44.037 327 172 6 3 324 1 321 9.37e-94 283
MS.gene48635.t1 MTR_5g074600 45.397 315 164 5 11 323 11 319 2.17e-93 282
MS.gene48635.t1 MTR_5g074580 43.082 318 173 5 4 319 5 316 3.42e-87 266
MS.gene48635.t1 MTR_4g088340 46.644 298 150 4 24 320 1 290 2.07e-85 261
MS.gene48635.t1 MTR_5g074610 42.071 309 146 3 11 319 7 282 2.73e-82 253
MS.gene48635.t1 MTR_5g074540 40.514 311 159 5 11 319 11 297 1.38e-81 251
MS.gene48635.t1 MTR_4g079160 41.290 310 173 3 11 319 10 311 1.49e-80 249
MS.gene48635.t1 MTR_1g076930 45.267 243 124 5 24 263 1 237 7.57e-61 195
MS.gene48635.t1 MTR_3g083620 34.202 307 186 9 20 323 27 320 1.70e-45 159
MS.gene48635.t1 MTR_0017s0100 32.602 319 204 6 3 319 14 323 5.19e-45 157
MS.gene48635.t1 MTR_4g038440 32.817 323 198 8 3 319 14 323 1.93e-44 156
MS.gene48635.t1 MTR_0079s0070 33.110 299 181 8 27 319 4 289 2.21e-40 144
MS.gene48635.t1 MTR_5g098170 34.201 269 163 9 29 290 31 292 3.01e-37 137
MS.gene48635.t1 MTR_3g092900 31.677 322 187 10 9 318 17 317 1.24e-33 127
MS.gene48635.t1 MTR_1g096310 30.218 321 206 8 5 318 17 326 4.00e-33 126
MS.gene48635.t1 MTR_0591s0010 30.961 281 182 7 26 301 41 314 1.12e-32 125
MS.gene48635.t1 MTR_7g118320 31.852 270 175 6 26 290 26 291 1.09e-31 122
MS.gene48635.t1 MTR_8g068870 29.097 299 200 7 5 298 6 297 2.61e-31 121
MS.gene48635.t1 MTR_7g118300 30.325 277 180 8 26 298 42 309 4.74e-31 120
MS.gene48635.t1 MTR_7g012090 29.137 278 178 7 32 298 40 309 1.03e-30 119
MS.gene48635.t1 MTR_1g036460 29.730 296 194 7 24 312 29 317 1.05e-30 119
MS.gene48635.t1 MTR_1g036490 30.100 299 189 9 24 312 29 317 1.46e-30 119
MS.gene48635.t1 MTR_8g068690 30.249 281 184 7 26 301 40 313 3.36e-30 118
MS.gene48635.t1 MTR_7g011900 29.964 277 182 7 32 303 40 309 4.46e-30 118
MS.gene48635.t1 MTR_7g012040 29.562 274 176 9 32 298 39 302 9.46e-30 117
MS.gene48635.t1 MTR_7g012070 29.242 277 179 7 32 298 40 309 1.44e-29 116
MS.gene48635.t1 MTR_8g068750 29.617 287 190 7 26 307 41 320 3.63e-29 115
MS.gene48635.t1 MTR_4g038440 30.712 267 169 6 3 263 14 270 1.16e-28 112
MS.gene48635.t1 MTR_2g072790 32.660 297 177 9 31 319 27 308 1.58e-28 113
MS.gene48635.t1 MTR_7g011990 27.660 282 185 9 32 306 40 309 2.07e-28 113
MS.gene48635.t1 MTR_2g055940 30.216 278 173 9 32 300 34 299 3.58e-28 112
MS.gene48635.t1 MTR_7g012020 29.044 272 181 7 32 298 39 303 3.65e-28 112
MS.gene48635.t1 MTR_8g068570 30.357 280 158 8 26 301 41 287 2.01e-27 109
MS.gene48635.t1 MTR_3g021440 29.492 295 197 7 28 318 38 325 3.42e-26 107
MS.gene48635.t1 MTR_1g036510 28.428 299 194 9 24 312 43 331 4.35e-26 107
MS.gene48635.t1 MTR_8g024160 33.663 202 120 6 101 298 71 262 6.27e-26 105
MS.gene48635.t1 MTR_3g021430 28.912 294 192 9 32 318 29 312 5.26e-24 101
MS.gene48635.t1 MTR_6g078850 25.952 289 198 7 9 290 33 312 6.37e-23 99.4
MS.gene48635.t1 MTR_7g012020 28.512 242 162 7 32 267 39 275 2.64e-21 92.8
MS.gene48635.t1 MTR_3g096050 26.733 303 202 10 23 319 24 312 1.91e-20 91.3
MS.gene48635.t1 MTR_8g068730 29.259 270 150 7 26 290 41 274 7.46e-20 89.4
MS.gene48635.t1 MTR_3g096070 29.213 178 120 3 143 319 117 289 2.56e-18 84.7
MS.gene48635.t1 MTR_8g068690 26.829 246 171 6 26 266 40 281 3.86e-18 83.6
MS.gene48635.t1 MTR_8g068740 27.737 274 155 8 26 292 41 278 8.79e-17 79.7
MS.gene48635.t1 MTR_1g036510 28.112 249 162 8 25 263 44 285 1.05e-16 79.7
MS.gene48635.t1 MTR_8g068600 26.293 232 162 6 38 264 27 254 3.38e-16 78.2
MS.gene48635.t1 MTR_8g068620 33.929 112 72 1 155 266 4 113 4.34e-14 68.2
MS.gene48635.t1 MTR_7g012020 27.619 210 143 6 32 236 39 244 2.19e-13 69.3
MS.gene48635.t1 MTR_4g025570 37.255 102 54 3 6 107 16 107 6.34e-13 67.4
MS.gene48635.t1 MTR_1g050220 33.333 120 67 2 193 312 146 252 1.16e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48635.t1 AT5G54160 32.414 290 186 6 31 318 34 315 1.50e-36 135
MS.gene48635.t1 AT4G35150 30.573 314 159 9 16 319 15 279 1.22e-34 129
MS.gene48635.t1 AT1G77520 33.813 278 167 8 31 298 44 314 3.64e-34 129
MS.gene48635.t1 AT4G35160 32.099 324 192 11 16 323 29 340 1.31e-32 125
MS.gene48635.t1 AT3G53140 31.457 302 179 10 31 319 27 313 1.82e-29 116
MS.gene48635.t1 AT5G53810 31.915 282 174 7 29 299 38 312 4.58e-27 110
MS.gene48635.t1 AT1G63140 29.545 308 197 9 9 303 19 319 4.76e-27 110
MS.gene48635.t1 AT1G33030 28.136 295 196 8 31 319 21 305 5.59e-27 109
MS.gene48635.t1 AT1G63140 30.225 311 191 11 9 303 39 339 6.02e-27 110
MS.gene48635.t1 AT1G77530 32.258 279 172 7 31 299 44 315 1.95e-26 108
MS.gene48635.t1 AT1G51990 29.167 288 187 8 33 315 32 307 2.13e-26 107
MS.gene48635.t1 AT1G51990 29.167 288 187 8 33 315 32 307 2.34e-25 105
MS.gene48635.t1 AT1G76790 26.888 331 203 11 7 319 9 318 9.85e-21 92.0
MS.gene48635.t1 AT1G62900 38.060 134 74 3 173 303 16 143 2.38e-19 85.1
MS.gene48635.t1 AT5G37170 31.373 153 94 5 172 319 43 189 4.01e-16 77.0
MS.gene48635.t1 AT1G21130 24.834 302 199 9 31 319 40 326 5.41e-16 78.6
MS.gene48635.t1 AT5G37170 31.373 153 94 5 172 319 143 289 7.45e-16 77.8
MS.gene48635.t1 AT1G21120 24.503 302 200 9 31 319 40 326 8.11e-16 78.2
MS.gene48635.t1 AT1G21120 24.503 302 200 9 31 319 88 374 9.10e-16 78.2
MS.gene48635.t1 AT1G21100 24.834 302 199 9 31 319 40 326 2.04e-15 76.6
MS.gene48635.t1 AT5G37170 32.576 132 81 4 193 319 13 141 3.14e-15 73.6
MS.gene48635.t1 AT1G21110 23.510 302 203 9 31 319 40 326 4.35e-15 75.9
MS.gene48635.t1 AT1G63140 28.044 271 172 10 9 263 39 302 1.58e-14 73.6
MS.gene48635.t1 AT1G33030 26.778 239 162 7 31 263 21 252 3.61e-14 72.0
MS.gene48635.t1 AT1G33030 26.778 239 162 7 31 263 21 252 7.41e-14 71.2

Find 67 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATACATGTTTGTATAAA+TGG 0.173388 4.1:+67786000 None:intergenic
TTCAAAGATGAGGCTAATTC+AGG 0.265492 4.1:+67785891 None:intergenic
TATAGGACACTAACTTTAGA+AGG 0.276793 4.1:+67785861 None:intergenic
CATCCATGTGTTTGAGGGTT+TGG 0.282085 4.1:-67785478 MS.gene48635:CDS
GTTTGAGGGTTTGGAGTCTT+TGG 0.303653 4.1:-67785469 MS.gene48635:CDS
CTCAGAGAACCATTTCTTAG+AGG 0.305502 4.1:+67785647 None:intergenic
AACAGCAAAGAAGCTATTTC+AGG 0.318216 4.1:-67784644 MS.gene48635:CDS
GTTGATGTGGCAGGTGGGAC+TGG 0.337992 4.1:-67785447 MS.gene48635:CDS
CTAAAGTTAGTGTCCTATAT+AGG 0.347448 4.1:-67785857 MS.gene48635:CDS
AAAGAAGCTATTTCAGGAAA+AGG 0.388596 4.1:-67784638 MS.gene48635:CDS
CCACTGATGAGAAGCTTTGA+AGG 0.393766 4.1:+67785738 None:intergenic
CAGGTTGTGGCTAACTTATC+TGG 0.396383 4.1:-67785360 MS.gene48635:CDS
AATTCTTCATCCATGTTCTC+TGG 0.398747 4.1:-67785682 MS.gene48635:CDS
TCATAGGCTTTCCATGTTTG+TGG 0.399025 4.1:+67785919 None:intergenic
GATAAACTTAGTTTGTTTCA+AGG 0.401061 4.1:-67785549 MS.gene48635:CDS
GCACTCTATGAAAGTGCAAT+GGG 0.425564 4.1:-67785612 MS.gene48635:CDS
CAAAATTTGAACTTTGTTGG+TGG 0.427047 4.1:-67785333 MS.gene48635:CDS
ACTCAAAATTTGAACTTTGT+TGG 0.429435 4.1:-67785336 MS.gene48635:CDS
GCTAATTCAGGAACAGTCAT+AGG 0.442962 4.1:+67785903 None:intergenic
GATTGCATCCATGTGTTTGA+GGG 0.448472 4.1:-67785483 MS.gene48635:CDS
AATGTTCAAGCTTGCTCTCA+AGG 0.450410 4.1:-67785505 MS.gene48635:CDS
TGTGGCACTCCTCTAAGAAA+TGG 0.452397 4.1:-67785656 MS.gene48635:CDS
ATAAACTTAGTTTGTTTCAA+GGG 0.456335 4.1:-67785548 MS.gene48635:CDS
ACAACGGTGAAAGGCAAAGA+AGG 0.456389 4.1:-67785795 MS.gene48635:CDS
GCAAGCGCAAGAACCTATAT+AGG 0.464145 4.1:+67785844 None:intergenic
AGAGACTTGAAGCCACATAC+TGG 0.465897 4.1:+67784431 None:intergenic
GGATTGCATCCATGTGTTTG+AGG 0.475962 4.1:-67785484 MS.gene48635:CDS
GAGTCTTTGGTTGATGTGGC+AGG 0.479885 4.1:-67785456 MS.gene48635:CDS
CTCAAATCTGCCGTGGAACT+AGG 0.483120 4.1:-67785957 MS.gene48635:CDS
AAGAAGCTATTTCAGGAAAA+GGG 0.484470 4.1:-67784637 MS.gene48635:CDS
ATCCATGTTCTCTGGATATG+TGG 0.500274 4.1:-67785674 MS.gene48635:CDS
TCTTTGGTTGATGTGGCAGG+TGG 0.504535 4.1:-67785453 MS.gene48635:CDS
TTTAGATCAACCACAGGTTG+TGG 0.518679 4.1:-67785373 MS.gene48635:CDS
CACGGCAGATTTGAGTGCCA+TGG 0.532354 4.1:+67785965 None:intergenic
TGCACTCTATGAAAGTGCAA+TGG 0.533425 4.1:-67785613 MS.gene48635:CDS
ATCTACTATGCCTAGTTCCA+CGG 0.544572 4.1:+67785947 None:intergenic
CAACGGTGAAAGGCAAAGAA+GGG 0.548081 4.1:-67785794 MS.gene48635:CDS
GAAATGGTTCTCTGAGGAGA+AGG 0.549990 4.1:-67785640 MS.gene48635:CDS
TTTGGAGTCTTTGGTTGATG+TGG 0.555368 4.1:-67785460 MS.gene48635:CDS
CAAATGGATTTCAAGAGCAA+TGG 0.555834 4.1:-67786056 MS.gene48635:CDS
TTGAGTAAAACAGCATCTGC+AGG 0.557051 4.1:+67785288 None:intergenic
TACAAGATTACTCCAGTATG+TGG 0.560046 4.1:-67784443 MS.gene48635:CDS
TAAACTTAGTTTGTTTCAAG+GGG 0.566712 4.1:-67785547 MS.gene48635:CDS
ATACAGCTTTATAAGTTCCA+TGG 0.573273 4.1:-67785982 MS.gene48635:CDS
CCTTCAAAGCTTCTCATCAG+TGG 0.578749 4.1:-67785738 MS.gene48635:CDS
CTTTGGTTGATGTGGCAGGT+GGG 0.578821 4.1:-67785452 MS.gene48635:CDS
GTTGCAATTGCACTTTGACA+TGG 0.578956 4.1:-67784546 MS.gene48635:intron
TGCCACATATCCAGAGAACA+TGG 0.593078 4.1:+67785672 None:intergenic
CATTTGTGAATCAGCTGCCA+TGG 0.594995 4.1:+67785524 None:intergenic
GTCAAAGTGCAATTGCAACT+CGG 0.597693 4.1:+67784550 None:intergenic
TAGTTTGTTTCAAGGGGCCA+TGG 0.599443 4.1:-67785541 MS.gene48635:CDS
AGGGTGAAGTTTCAAAGATG+AGG 0.607728 4.1:+67785881 None:intergenic
GATAAGTTAGCCACAACCTG+TGG 0.609880 4.1:+67785363 None:intergenic
CATGGCACTCAAATCTGCCG+TGG 0.617925 4.1:-67785964 MS.gene48635:CDS
ACTCCAAACCCTCAAACACA+TGG 0.624325 4.1:+67785475 None:intergenic
AGTAAAACAGCATCTGCAGG+AGG 0.624714 4.1:+67785291 None:intergenic
GTAGATGCAATCCACAAACA+TGG 0.627357 4.1:-67785930 MS.gene48635:CDS
ATAGGACACTAACTTTAGAA+GGG 0.629455 4.1:+67785862 None:intergenic
CACTCTATGAAAGTGCAATG+GGG 0.635539 4.1:-67785611 MS.gene48635:CDS
AACGGTGAAAGGCAAAGAAG+GGG 0.637209 4.1:-67785793 MS.gene48635:CDS
ACTCTATGAAAGTGCAATGG+GGG 0.643797 4.1:-67785610 MS.gene48635:CDS
TTGCGCTTGCTAACACACGA+TGG 0.651331 4.1:-67785831 MS.gene48635:CDS
AAAACTGTGCATTTGATGTG+AGG 0.654544 4.1:+67785393 None:intergenic
TGAAGAATTCCCTTAACAAG+TGG 0.661082 4.1:+67785696 None:intergenic
CTGATGAGAAGCTTTGAAGG+AGG 0.668837 4.1:+67785741 None:intergenic
CTCTAAGAAATGGTTCTCTG+AGG 0.677453 4.1:-67785646 MS.gene48635:CDS
GTAAAACAGCATCTGCAGGA+GGG 0.681270 4.1:+67785292 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATTATTTTAATTTAT+TGG - chr4.1:67785723-67785742 MS.gene48635:CDS 0.0%
!! TTTGTTAAGAAAGTTAAAAA+AGG + chr4.1:67784898-67784917 None:intergenic 15.0%
!! CATATATGGACAAAATAAAA+TGG + chr4.1:67785256-67785275 None:intergenic 20.0%
!! TATGAAATCATGAACATATA+TGG + chr4.1:67785270-67785289 None:intergenic 20.0%
!! TGTATACATGTTTGTATAAA+TGG + chr4.1:67784482-67784501 None:intergenic 20.0%
!!! ATAAACTTAGTTTGTTTCAA+GGG - chr4.1:67784931-67784950 MS.gene48635:intron 20.0%
!!! ATCAAGAAATTTTTACTTGT+GGG - chr4.1:67785302-67785321 MS.gene48635:CDS 20.0%
!!! TATCAAGAAATTTTTACTTG+TGG - chr4.1:67785301-67785320 MS.gene48635:CDS 20.0%
! AAGAAAGAGAAAAGAAAGAA+TGG - chr4.1:67785980-67785999 MS.gene48635:CDS 25.0%
! ACTCAAAATTTGAACTTTGT+TGG - chr4.1:67785143-67785162 MS.gene48635:intron 25.0%
! ATGTTCATGATTTCATAATC+AGG - chr4.1:67785273-67785292 MS.gene48635:intron 25.0%
!! TCAAGAAATTTTTACTTGTG+GGG - chr4.1:67785303-67785322 MS.gene48635:CDS 25.0%
!!! GATAAACTTAGTTTGTTTCA+AGG - chr4.1:67784930-67784949 MS.gene48635:intron 25.0%
!!! TAAACTTAGTTTGTTTCAAG+GGG - chr4.1:67784932-67784951 MS.gene48635:intron 25.0%
ATCTCAAATGTCTTTCAAAG+TGG - chr4.1:67785767-67785786 MS.gene48635:CDS 30.0%
ATTTCAGGAAAAGGGAAAAA+AGG - chr4.1:67785850-67785869 MS.gene48635:CDS 30.0%
CAAAATTTGAACTTTGTTGG+TGG - chr4.1:67785146-67785165 MS.gene48635:intron 30.0%
CTAAAGTTAGTGTCCTATAT+AGG - chr4.1:67784622-67784641 MS.gene48635:CDS 30.0%
CTTAACTCAAAAAGTTACGA+CGG + chr4.1:67785652-67785671 None:intergenic 30.0%
TATAGGACACTAACTTTAGA+AGG + chr4.1:67784621-67784640 None:intergenic 30.0%
TCTCAAATGTCTTTCAAAGT+GGG - chr4.1:67785768-67785787 MS.gene48635:CDS 30.0%
! AAAGAAGCTATTTCAGGAAA+AGG - chr4.1:67785841-67785860 MS.gene48635:CDS 30.0%
! AAGAAGCTATTTCAGGAAAA+GGG - chr4.1:67785842-67785861 MS.gene48635:CDS 30.0%
! ATACAGCTTTATAAGTTCCA+TGG - chr4.1:67784497-67784516 MS.gene48635:intron 30.0%
! ATAGGACACTAACTTTAGAA+GGG + chr4.1:67784620-67784639 None:intergenic 30.0%
AAAACTGTGCATTTGATGTG+AGG + chr4.1:67785089-67785108 None:intergenic 35.0%
AAGAAACTCATATATGACGC+AGG - chr4.1:67786003-67786022 MS.gene48635:CDS 35.0%
AATTCTTCATCCATGTTCTC+TGG - chr4.1:67784797-67784816 MS.gene48635:intron 35.0%
GTAATGATGACTCTGCATAA+TGG - chr4.1:67785955-67785974 MS.gene48635:CDS 35.0%
TACAAGATTACTCCAGTATG+TGG - chr4.1:67786036-67786055 MS.gene48635:CDS 35.0%
TGAAGAATTCCCTTAACAAG+TGG + chr4.1:67784786-67784805 None:intergenic 35.0%
TGGTTTCTTCGCAAAAACAA+CGG - chr4.1:67784668-67784687 MS.gene48635:CDS 35.0%
TTCAAAGATGAGGCTAATTC+AGG + chr4.1:67784591-67784610 None:intergenic 35.0%
! AACAGCAAAGAAGCTATTTC+AGG - chr4.1:67785835-67785854 MS.gene48635:CDS 35.0%
!! AAAGTGGGTTTTGCATGATT+GGG - chr4.1:67785783-67785802 MS.gene48635:CDS 35.0%
!! TGTTTTGCACCACTTGTTAA+GGG - chr4.1:67784774-67784793 MS.gene48635:intron 35.0%
!! TTGTTTTGCACCACTTGTTA+AGG - chr4.1:67784773-67784792 MS.gene48635:intron 35.0%
AGGGTGAAGTTTCAAAGATG+AGG + chr4.1:67784601-67784620 None:intergenic 40.0%
ATCCAGTCACATGTGATTGA+GGG + chr4.1:67785606-67785625 None:intergenic 40.0%
ATCCATGTTCTCTGGATATG+TGG - chr4.1:67784805-67784824 MS.gene48635:intron 40.0%
ATCTACTATGCCTAGTTCCA+CGG + chr4.1:67784535-67784554 None:intergenic 40.0%
CTCAGAGAACCATTTCTTAG+AGG + chr4.1:67784835-67784854 None:intergenic 40.0%
GATTGCATCCATGTGTTTGA+GGG - chr4.1:67784996-67785015 MS.gene48635:intron 40.0%
GCTAATTCAGGAACAGTCAT+AGG + chr4.1:67784579-67784598 None:intergenic 40.0%
GTAGATGCAATCCACAAACA+TGG - chr4.1:67784549-67784568 MS.gene48635:CDS 40.0%
GTCAAAGTGCAATTGCAACT+CGG + chr4.1:67785932-67785951 None:intergenic 40.0%
GTTGCAATTGCACTTTGACA+TGG - chr4.1:67785933-67785952 MS.gene48635:CDS 40.0%
TCATAGGCTTTCCATGTTTG+TGG + chr4.1:67784563-67784582 None:intergenic 40.0%
TTCGCAAAAACAACGGTGAA+AGG - chr4.1:67784675-67784694 MS.gene48635:CDS 40.0%
TTGAGTAAAACAGCATCTGC+AGG + chr4.1:67785194-67785213 None:intergenic 40.0%
TTTAGATCAACCACAGGTTG+TGG - chr4.1:67785106-67785125 MS.gene48635:intron 40.0%
! AATGTTCAAGCTTGCTCTCA+AGG - chr4.1:67784974-67784993 MS.gene48635:intron 40.0%
! ACTCTATGAAAGTGCAATGG+GGG - chr4.1:67784869-67784888 MS.gene48635:intron 40.0%
! ATTGAGGGCACATACATAGA+GGG + chr4.1:67785591-67785610 None:intergenic 40.0%
! CACTCTATGAAAGTGCAATG+GGG - chr4.1:67784868-67784887 MS.gene48635:intron 40.0%
! CTCTAAGAAATGGTTCTCTG+AGG - chr4.1:67784833-67784852 MS.gene48635:intron 40.0%
! GCACTCTATGAAAGTGCAAT+GGG - chr4.1:67784867-67784886 MS.gene48635:intron 40.0%
! TGCACTCTATGAAAGTGCAA+TGG - chr4.1:67784866-67784885 MS.gene48635:intron 40.0%
! TTTGGAGTCTTTGGTTGATG+TGG - chr4.1:67785019-67785038 MS.gene48635:intron 40.0%
!! CAAAGTGGGTTTTGCATGAT+TGG - chr4.1:67785782-67785801 MS.gene48635:CDS 40.0%
!!! CACAGTTTTAGATCAACCAC+AGG - chr4.1:67785100-67785119 MS.gene48635:intron 40.0%
!!! TGCAGATGCTGTTTTACTCA+AGG - chr4.1:67785193-67785212 MS.gene48635:intron 40.0%
AACGGTGAAAGGCAAAGAAG+GGG - chr4.1:67784686-67784705 MS.gene48635:CDS 45.0%
ACAACGGTGAAAGGCAAAGA+AGG - chr4.1:67784684-67784703 MS.gene48635:CDS 45.0%
ACTCCAAACCCTCAAACACA+TGG + chr4.1:67785007-67785026 None:intergenic 45.0%
AGAGACTTGAAGCCACATAC+TGG + chr4.1:67786051-67786070 None:intergenic 45.0%
AGTAAAACAGCATCTGCAGG+AGG + chr4.1:67785191-67785210 None:intergenic 45.0%
CAACGGTGAAAGGCAAAGAA+GGG - chr4.1:67784685-67784704 MS.gene48635:CDS 45.0%
CAGGTTGTGGCTAACTTATC+TGG - chr4.1:67785119-67785138 MS.gene48635:intron 45.0%
CATCCATGTGTTTGAGGGTT+TGG - chr4.1:67785001-67785020 MS.gene48635:intron 45.0%
CATTTGTGAATCAGCTGCCA+TGG + chr4.1:67784958-67784977 None:intergenic 45.0%
CCTTCAAAGCTTCTCATCAG+TGG - chr4.1:67784741-67784760 MS.gene48635:intron 45.0%
GATAAGTTAGCCACAACCTG+TGG + chr4.1:67785119-67785138 None:intergenic 45.0%
GATCCAGTCACATGTGATTG+AGG + chr4.1:67785607-67785626 None:intergenic 45.0%
GCAAGCGCAAGAACCTATAT+AGG + chr4.1:67784638-67784657 None:intergenic 45.0%
GGATTGCATCCATGTGTTTG+AGG - chr4.1:67784995-67785014 MS.gene48635:intron 45.0%
GTAAAACAGCATCTGCAGGA+GGG + chr4.1:67785190-67785209 None:intergenic 45.0%
TGCCACATATCCAGAGAACA+TGG + chr4.1:67784810-67784829 None:intergenic 45.0%
! CCACTGATGAGAAGCTTTGA+AGG + chr4.1:67784744-67784763 None:intergenic 45.0%
! GAAATGGTTCTCTGAGGAGA+AGG - chr4.1:67784839-67784858 MS.gene48635:intron 45.0%
! GATTGAGGGCACATACATAG+AGG + chr4.1:67785592-67785611 None:intergenic 45.0%
! GTTTGAGGGTTTGGAGTCTT+TGG - chr4.1:67785010-67785029 MS.gene48635:intron 45.0%
! TGTGGCACTCCTCTAAGAAA+TGG - chr4.1:67784823-67784842 MS.gene48635:intron 45.0%
!! CTGATGAGAAGCTTTGAAGG+AGG + chr4.1:67784741-67784760 None:intergenic 45.0%
!! TAGTTTGTTTCAAGGGGCCA+TGG - chr4.1:67784938-67784957 MS.gene48635:intron 45.0%
CTCAAATCTGCCGTGGAACT+AGG - chr4.1:67784522-67784541 MS.gene48635:intron 50.0%
TGCCCTCAATCACATGTGAC+TGG - chr4.1:67785601-67785620 MS.gene48635:CDS 50.0%
! CTTTGGTTGATGTGGCAGGT+GGG - chr4.1:67785027-67785046 MS.gene48635:intron 50.0%
! GAGTCTTTGGTTGATGTGGC+AGG - chr4.1:67785023-67785042 MS.gene48635:intron 50.0%
! TCTTTGGTTGATGTGGCAGG+TGG - chr4.1:67785026-67785045 MS.gene48635:intron 50.0%
! TTGCGCTTGCTAACACACGA+TGG - chr4.1:67784648-67784667 MS.gene48635:CDS 50.0%
CACGGCAGATTTGAGTGCCA+TGG + chr4.1:67784517-67784536 None:intergenic 55.0%
CATGGCACTCAAATCTGCCG+TGG - chr4.1:67784515-67784534 MS.gene48635:intron 55.0%
GTTGATGTGGCAGGTGGGAC+TGG - chr4.1:67785032-67785051 MS.gene48635:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 67784426 67786075 67784426 ID=MS.gene48635
chr4.1 mRNA 67784426 67786075 67784426 ID=MS.gene48635.t1;Parent=MS.gene48635
chr4.1 exon 67785287 67786075 67785287 ID=MS.gene48635.t1.exon1;Parent=MS.gene48635.t1
chr4.1 CDS 67785287 67786075 67785287 ID=cds.MS.gene48635.t1;Parent=MS.gene48635.t1
chr4.1 exon 67784547 67784714 67784547 ID=MS.gene48635.t1.exon2;Parent=MS.gene48635.t1
chr4.1 CDS 67784547 67784714 67784547 ID=cds.MS.gene48635.t1;Parent=MS.gene48635.t1
chr4.1 exon 67784426 67784476 67784426 ID=MS.gene48635.t1.exon3;Parent=MS.gene48635.t1
chr4.1 CDS 67784426 67784476 67784426 ID=cds.MS.gene48635.t1;Parent=MS.gene48635.t1
Gene Sequence

>MS.gene48635

ATGGATTTCAAGAGCAATGGCAGTGAAGAAACTCAGCTGTATCATGCTCAAATCCATTTATACAAACATGTATACAGCTTTATAAGTTCCATGGCACTCAAATCTGCCGTGGAACTAGGCATAGTAGATGCAATCCACAAACATGGAAAGCCTATGACTGTTCCTGAATTAGCCTCATCTTTGAAACTTCACCCTTCTAAAGTTAGTGTCCTATATAGGTTCTTGCGCTTGCTAACACACGATGGTTTCTTCGCAAAAACAACGGTGAAAGGCAAAGAAGGGGAAGAAGAAGAAACAGCTTATGCTCTCACTCCTCCTTCAAAGCTTCTCATCAGTGGTGAATCAACTTGTTTTGCACCACTTGTTAAGGGAATTCTTCATCCATGTTCTCTGGATATGTGGCACTCCTCTAAGAAATGGTTCTCTGAGGAGAAGGATCTTGCACTCTATGAAAGTGCAATGGGGGAGACCTTTTTTAACTTTCTTAACAAAGATTCTGAATCTGATAAACTTAGTTTGTTTCAAGGGGCCATGGCAGCTGATTCACAAATGTTCAAGCTTGCTCTCAAGGATTGCATCCATGTGTTTGAGGGTTTGGAGTCTTTGGTTGATGTGGCAGGTGGGACTGGAGTTGTGTCAAAACTCATTCATGAAGAATTTCCTCACATCAAATGCACAGTTTTAGATCAACCACAGGTTGTGGCTAACTTATCTGGAACTCAAAATTTGAACTTTGTTGGTGGAGATATGTTCAAATCTATCCCTCCTGCAGATGCTGTTTTACTCAAGGTATGTTTATGTTTATCATTTTCTACTATTTGATCATTCCATTTTATTTTGTCCATATATGTTCATGATTTCATAATCAGGATCAGTATCAAGAAATTTTTACTTGTGGGGCAAGATTTTATATAATAAATATTTATACAAAATTTTTAGCAATGTGTAAGTAATAAATTAAAATCATTAGTTTTTTTTACTAAAAAATAATATCGTTATCATTGAGAAAAATACTTGAGATTTTAAACACTACACTATTATATTTAGAAAAATTAAAGAGTCATTAACATCTAAAAATTAAAATTGTTCTTTATATATGTGTCTTTTTATAATTAAAAAAGTATCATAGAATTTTGCTCATATTTAGCACTATCAAAAATATCCCTCTATGTATGTGCCCTCAATCACATGTGACTGGATCTGTGTCTGATTAAGATATAAATCCGTCGTAACTTTTTGAGTTAAGATCTTCTCTGTTTAATAGACATTTCAATTTTGAAAGAAATAAAATTGTAAAATAATATTATTTTAATTTATTGGTCAATTTAACATTGTACTAAAATCTCAAATGTCTTTCAAAGTGGGTTTTGCATGATTGGGATGATGAGCTCTCTTTAAAGATATTGAAGAACAGCAAAGAAGCTATTTCAGGAAAAGGGAAAAAAGGAAAGATAATAATCATAGACATATCAATTGATGAAGCAAGTGATGATCGTGAATTAACCGAGTTGCAATTGCACTTTGACATGGTAATGATGACTCTGCATAATGGAAAAGAAAGAGAAAAGAAAGAATGGAAGAAACTCATATATGACGCAGGCTTCAGCAGCTACAAGATTACTCCAGTATGTGGCTTCAAGTCTCTCATAG
Protein sequence

>MS.gene48635.t1

MDFKSNGSEETQLYHAQIHLYKHVYSFISSMALKSAVELGIVDAIHKHGKPMTVPELASSLKLHPSKVSVLYRFLRLLTHDGFFAKTTVKGKEGEEEETAYALTPPSKLLISGESTCFAPLVKGILHPCSLDMWHSSKKWFSEEKDLALYESAMGETFFNFLNKDSESDKLSLFQGAMAADSQMFKLALKDCIHVFEGLESLVDVAGGTGVVSKLIHEEFPHIKCTVLDQPQVVANLSGTQNLNFVGGDMFKSIPPADAVLLKWVLHDWDDELSLKILKNSKEAISGKGKKGKIIIIDISIDEASDDRELTELQLHFDMASAATRLLQYVASSLS