AlfalfaGEDB | MS.gene48663

M. sativa cultivar XinJiangDaYe / MS.gene48663

KEGG: K02881
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00861.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48663.t1	XP_003605528.1	97	101	3	0	1	101	99	199	4.80E-47	196.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48663.t1	Q5P316	34.4	93	60	1	8	100	26	117	2.2e-08	59.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48663.t1	G7JN88	97.0	101	3	0	1	101	99	199	3.4e-47	196.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055748	MS.gene48663	0.804695	1.91E-49	-1.69E-46
MS.gene057831	MS.gene48663	0.807317	5.36E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48663.t1	MTR_4g032660	97.030	101	3	0	1	101	99	199	6.04e-70	207
MS.gene48663.t1	MTR_3g012540	48.454	97	50	0	2	98	84	180	3.11e-28	101
MS.gene48663.t1	MTR_5g006310	41.237	97	57	0	2	98	240	336	1.49e-21	87.4
MS.gene48663.t1	MTR_1g114200	32.990	97	65	0	2	98	15	111	1.42e-17	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48663.t1	AT3G20230	73.267	101	27	0	1	101	86	186	1.49e-54	168
MS.gene48663.t1	AT1G08845	46.392	97	52	0	2	98	86	182	5.23e-28	100
MS.gene48663.t1	AT1G08845	46.392	97	52	0	2	98	86	182	5.23e-28	100
MS.gene48663.t1	AT1G08845	46.392	97	52	0	2	98	131	227	7.13e-28	101
MS.gene48663.t1	AT5G27820	34.021	97	64	0	2	98	15	111	6.67e-19	75.5
MS.gene48663.t1	AT3G22450	42.553	94	54	0	5	98	197	290	5.37e-15	68.9
MS.gene48663.t1	AT3G22450	42.553	94	54	0	5	98	197	290	5.37e-15	68.9

Find 18 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTGAAGCTTGTAGAGTAAT+TGG	0.243088	4.3:-67931973	MS.gene48663:CDS
TCAATCCTTTCATTCTTTCT+CGG	0.316725	4.3:+67931895	None:intergenic
CCAATTACTCTACAAGCTTC+AGG	0.324712	4.3:+67931973	None:intergenic
AAGAGAGTTTCGAAGATCTT+TGG	0.330634	4.3:+67932011	None:intergenic
CTTGATACTGTTAAAGAAAA+CGG	0.356290	4.3:-67931853	MS.gene48663:CDS
TTGATACTGTTAAAGAAAAC+GGG	0.383944	4.3:-67931852	MS.gene48663:CDS
TGAAAGGATTGAAGGTAGAC+TGG	0.403984	4.3:-67931884	MS.gene48663:CDS
TCGAAGATCTTTGGCGTTTG+TGG	0.436613	4.3:+67932020	None:intergenic
AGAAAGAATGAAAGGATTGA+AGG	0.478137	4.3:-67931892	MS.gene48663:CDS
ATATCACTTTGCTTGTTCCA+CGG	0.510627	4.3:+67932052	None:intergenic
ACAAGCTTCAGGATCTATCA+AGG	0.541521	4.3:+67931984	None:intergenic
GCTTCAGGATCTATCAAGGA+TGG	0.567230	4.3:+67931988	None:intergenic
ACGAACCGAGAAAGAATGAA+AGG	0.579793	4.3:-67931900	MS.gene48663:CDS
GCTTATAGCAGAGCGATCAA+AGG	0.589698	4.3:-67931947	MS.gene48663:CDS
GAAAGGATTGAAGGTAGACT+GGG	0.594965	4.3:-67931883	MS.gene48663:CDS
GGAAGCTGATGTATATGCAA+TGG	0.597806	4.3:-67931926	MS.gene48663:CDS
AAGCTTGTAGAGTAATTGGA+AGG	0.614586	4.3:-67931969	MS.gene48663:CDS
CACGCGAAAGTGACACACCG+TGG	0.691365	4.3:-67932069	MS.gene48663:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAAAATGTCTAATAAAAA+CGG	+	chr4.3:67931852-67931871	None:intergenic	15.0%
!	CTTGATACTGTTAAAGAAAA+CGG	-	chr4.3:67932104-67932123	MS.gene48663:CDS	25.0%
!	TTGATACTGTTAAAGAAAAC+GGG	-	chr4.3:67932105-67932124	MS.gene48663:CDS	25.0%
	AGAAAGAATGAAAGGATTGA+AGG	-	chr4.3:67932065-67932084	MS.gene48663:CDS	30.0%
	TCAATCCTTTCATTCTTTCT+CGG	+	chr4.3:67932065-67932084	None:intergenic	30.0%
	AAGAGAGTTTCGAAGATCTT+TGG	+	chr4.3:67931949-67931968	None:intergenic	35.0%
	AAGCTTGTAGAGTAATTGGA+AGG	-	chr4.3:67931988-67932007	MS.gene48663:CDS	35.0%
	ATATCACTTTGCTTGTTCCA+CGG	+	chr4.3:67931908-67931927	None:intergenic	35.0%
	ACAAGCTTCAGGATCTATCA+AGG	+	chr4.3:67931976-67931995	None:intergenic	40.0%
	ACGAACCGAGAAAGAATGAA+AGG	-	chr4.3:67932057-67932076	MS.gene48663:CDS	40.0%
	CCAATTACTCTACAAGCTTC+AGG	+	chr4.3:67931987-67932006	None:intergenic	40.0%
	CCTGAAGCTTGTAGAGTAAT+TGG	-	chr4.3:67931984-67932003	MS.gene48663:CDS	40.0%
	GAAAGGATTGAAGGTAGACT+GGG	-	chr4.3:67932074-67932093	MS.gene48663:CDS	40.0%
	GGAAGCTGATGTATATGCAA+TGG	-	chr4.3:67932031-67932050	MS.gene48663:CDS	40.0%
	TGAAAGGATTGAAGGTAGAC+TGG	-	chr4.3:67932073-67932092	MS.gene48663:CDS	40.0%
	GCTTATAGCAGAGCGATCAA+AGG	-	chr4.3:67932010-67932029	MS.gene48663:CDS	45.0%
	GCTTCAGGATCTATCAAGGA+TGG	+	chr4.3:67931972-67931991	None:intergenic	45.0%
	TCGAAGATCTTTGGCGTTTG+TGG	+	chr4.3:67931940-67931959	None:intergenic	45.0%
	CACGCGAAAGTGACACACCG+TGG	-	chr4.3:67931888-67931907	MS.gene48663:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	67931837	67932142	67931837	ID=MS.gene48663
chr4.3	mRNA	67931837	67932142	67931837	ID=MS.gene48663.t1;Parent=MS.gene48663
chr4.3	exon	67931837	67932142	67931837	ID=MS.gene48663.t1.exon1;Parent=MS.gene48663.t1
chr4.3	CDS	67931837	67932142	67931837	ID=cds.MS.gene48663.t1;Parent=MS.gene48663.t1

Gene Sequence	>MS.gene48663 ATGTACATGAGACCGTTTTTATTAGACATTTTCTTTTCAAAGAAATTCGTTCACGCGAAAGTGACACACCGTGGAACAAGCAAAGTGATATGTGCTGCTACCACAAACGCCAAAGATCTTCGAAACTCTCTTCCATCCTTGATAGATCCTGAAGCTTGTAGAGTAATTGGAAGGCTTATAGCAGAGCGATCAAAGGAAGCTGATGTATATGCAATGGCATACGAACCGAGAAAGAATGAAAGGATTGAAGGTAGACTGGGAATCATTCTTGATACTGTTAAAGAAAACGGGATTCTTTTTGTTTGA
Protein sequence	>MS.gene48663.t1 MYMRPFLLDIFFSKKFVHAKVTHRGTSKVICAATTNAKDLRNSLPSLIDPEACRVIGRLIAERSKEADVYAMAYEPRKNERIEGRLGIILDTVKENGILFV

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