Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48753.t1 | XP_013467661.1 | 98.6 | 74 | 1 | 0 | 1 | 74 | 269 | 342 | 5.10E-30 | 139.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48753.t1 | A0A072VJS3 | 98.6 | 74 | 1 | 0 | 1 | 74 | 269 | 342 | 3.6e-30 | 139.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057932 | MS.gene48753 | 0.873392 | 1.52E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48753.t1 | MTR_1g053910 | 98.649 | 74 | 1 | 0 | 1 | 74 | 269 | 342 | 1.53e-44 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTGATGCGAGCAAGGAAT+TGG | 0.376840 | 4.4:-72920268 | MS.gene48753:CDS |
| GGAGGAGGGAAAAGCTTTGA+GGG | 0.400312 | 4.4:-72920351 | MS.gene48753:CDS |
| TGGAGGAGGGAAAAGCTTTG+AGG | 0.441086 | 4.4:-72920352 | MS.gene48753:CDS |
| AGAAAGAATGAGAAGAAAAC+CGG | 0.442575 | 4.4:-72920327 | MS.gene48753:CDS |
| GGAATGCTGTGGAGGATCTT+CGG | 0.449412 | 4.4:-72920398 | MS.gene48753:CDS |
| GGAGAGAATTCACTAGTGTA+AGG | 0.470518 | 4.4:-72920377 | MS.gene48753:CDS |
| GAGGCAAATGGAGGTTCTGC+AGG | 0.475327 | 4.4:-72920439 | None:intergenic |
| GAGGTTCTGCAGGCGGAGAA+TGG | 0.475648 | 4.4:-72920429 | MS.gene48753:CDS |
| GGAATTGGAGAAGGCGTTGA+GGG | 0.502532 | 4.4:-72920253 | MS.gene48753:CDS |
| TTGAGGGGAGCCACCACTGT+TGG | 0.513960 | 4.4:-72920237 | MS.gene48753:CDS |
| TTCACTAGTGTAAGGATTGG+AGG | 0.529400 | 4.4:-72920369 | MS.gene48753:CDS |
| GAATTCACTAGTGTAAGGAT+TGG | 0.533076 | 4.4:-72920372 | MS.gene48753:CDS |
| GACTGAGGCTGATGCGAGCA+AGG | 0.549803 | 4.4:-72920274 | MS.gene48753:CDS |
| ACTAGTGTAAGGATTGGAGG+AGG | 0.570999 | 4.4:-72920366 | MS.gene48753:CDS |
| TCAGCCTCAGTCGATTTCCC+AGG | 0.596668 | 4.4:+72920285 | None:intergenic |
| GAGGAGGGAAAAGCTTTGAG+GGG | 0.601233 | 4.4:-72920350 | MS.gene48753:CDS |
| AATTCAAGCTCCAACAGTGG+TGG | 0.602493 | 4.4:+72920227 | None:intergenic |
| TTGAATTCAAGCTCCAACAG+TGG | 0.608904 | 4.4:+72920224 | None:intergenic |
| TGCGAGCAAGGAATTGGAGA+AGG | 0.616295 | 4.4:-72920262 | MS.gene48753:CDS |
| AGGAATTGGAGAAGGCGTTG+AGG | 0.624601 | 4.4:-72920254 | MS.gene48753:CDS |
| GCAAATGGAGGTTCTGCAGG+CGG | 0.640904 | 4.4:-72920436 | None:intergenic |
| GAAAGAATGAGAAGAAAACC+GGG | 0.648044 | 4.4:-72920326 | MS.gene48753:CDS |
| GAATTGGAGAAGGCGTTGAG+GGG | 0.650714 | 4.4:-72920252 | MS.gene48753:CDS |
| AGGCGGAGAATGGATTGCTG+AGG | 0.664151 | 4.4:-72920419 | MS.gene48753:CDS |
| ATTGCTGAGGAATGCTGTGG+AGG | 0.665643 | 4.4:-72920406 | MS.gene48753:CDS |
| CTAGTGTAAGGATTGGAGGA+GGG | 0.671920 | 4.4:-72920365 | MS.gene48753:CDS |
| TGGATTGCTGAGGAATGCTG+TGG | 0.678621 | 4.4:-72920409 | MS.gene48753:CDS |
| ATAACCACTAAAATCTGACC+CGG | 0.690957 | 4.4:+72920308 | None:intergenic |
| TTATCCTGGGAAATCGACTG+AGG | 0.704241 | 4.4:-72920289 | MS.gene48753:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGAAAGAATGAGAAGAAAAC+CGG | - | chr4.4:72920335-72920354 | MS.gene48753:CDS | 30.0% | |
| ATAACCACTAAAATCTGACC+CGG | + | chr4.4:72920357-72920376 | None:intergenic | 35.0% | |
| GAAAGAATGAGAAGAAAACC+GGG | - | chr4.4:72920336-72920355 | MS.gene48753:CDS | 35.0% | |
| GAATTCACTAGTGTAAGGAT+TGG | - | chr4.4:72920290-72920309 | MS.gene48753:CDS | 35.0% | |
| !! | CAGATTTTAGTGGTTATCCT+GGG | - | chr4.4:72920360-72920379 | MS.gene48753:CDS | 35.0% |
| !! | TCAGATTTTAGTGGTTATCC+TGG | - | chr4.4:72920359-72920378 | MS.gene48753:CDS | 35.0% |
| GGAGAGAATTCACTAGTGTA+AGG | - | chr4.4:72920285-72920304 | MS.gene48753:CDS | 40.0% | |
| TTCACTAGTGTAAGGATTGG+AGG | - | chr4.4:72920293-72920312 | MS.gene48753:CDS | 40.0% | |
| ! | AAAACCGGGTCAGATTTTAG+TGG | - | chr4.4:72920350-72920369 | MS.gene48753:CDS | 40.0% |
| ACTAGTGTAAGGATTGGAGG+AGG | - | chr4.4:72920296-72920315 | MS.gene48753:CDS | 45.0% | |
| CTAGTGTAAGGATTGGAGGA+GGG | - | chr4.4:72920297-72920316 | MS.gene48753:CDS | 45.0% | |
| TTATCCTGGGAAATCGACTG+AGG | - | chr4.4:72920373-72920392 | MS.gene48753:CDS | 45.0% | |
| ATTGCTGAGGAATGCTGTGG+AGG | - | chr4.4:72920256-72920275 | MS.gene48753:CDS | 50.0% | |
| GGAATGCTGTGGAGGATCTT+CGG | - | chr4.4:72920264-72920283 | MS.gene48753:CDS | 50.0% | |
| TGCGAGCAAGGAATTGGAGA+AGG | - | chr4.4:72920400-72920419 | MS.gene48753:CDS | 50.0% | |
| TGGAGGAGGGAAAAGCTTTG+AGG | - | chr4.4:72920310-72920329 | MS.gene48753:CDS | 50.0% | |
| TGGATTGCTGAGGAATGCTG+TGG | - | chr4.4:72920253-72920272 | MS.gene48753:CDS | 50.0% | |
| ! | GGAGGAGGGAAAAGCTTTGA+GGG | - | chr4.4:72920311-72920330 | MS.gene48753:CDS | 50.0% |
| !! | AGGAATTGGAGAAGGCGTTG+AGG | - | chr4.4:72920408-72920427 | MS.gene48753:CDS | 50.0% |
| !! | GAATTGGAGAAGGCGTTGAG+GGG | - | chr4.4:72920410-72920429 | MS.gene48753:CDS | 50.0% |
| !! | GAGGAGGGAAAAGCTTTGAG+GGG | - | chr4.4:72920312-72920331 | MS.gene48753:CDS | 50.0% |
| !! | GGAATTGGAGAAGGCGTTGA+GGG | - | chr4.4:72920409-72920428 | MS.gene48753:CDS | 50.0% |
| GGCTGATGCGAGCAAGGAAT+TGG | - | chr4.4:72920394-72920413 | MS.gene48753:CDS | 55.0% | |
| ! | TCAGCCTCAGTCGATTTCCC+AGG | + | chr4.4:72920380-72920399 | None:intergenic | 55.0% |
| !! | AGGCGGAGAATGGATTGCTG+AGG | - | chr4.4:72920243-72920262 | MS.gene48753:CDS | 55.0% |
| GACTGAGGCTGATGCGAGCA+AGG | - | chr4.4:72920388-72920407 | MS.gene48753:CDS | 60.0% | |
| GAGGTTCTGCAGGCGGAGAA+TGG | - | chr4.4:72920233-72920252 | MS.gene48753:CDS | 60.0% | |
| TTGAGGGGAGCCACCACTGT+TGG | - | chr4.4:72920425-72920444 | MS.gene48753:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 72920230 | 72920454 | 72920230 | ID=MS.gene48753 |
| chr4.4 | mRNA | 72920230 | 72920454 | 72920230 | ID=MS.gene48753.t1;Parent=MS.gene48753 |
| chr4.4 | exon | 72920230 | 72920454 | 72920230 | ID=MS.gene48753.t1.exon1;Parent=MS.gene48753.t1 |
| chr4.4 | CDS | 72920230 | 72920454 | 72920230 | ID=cds.MS.gene48753.t1;Parent=MS.gene48753.t1 |
| Gene Sequence |
| Protein sequence |
