AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48800.t1 MTR_4g021815 95.556 90 4 0 1 90 85 174 2.28e-59 188
MS.gene48800.t1 MTR_7g062140 83.333 90 15 0 1 90 159 248 4.62e-51 169
MS.gene48800.t1 MTR_1g038793 82.759 87 15 0 146 232 407 493 2.31e-41 150
MS.gene48800.t1 MTR_1g038793 59.829 117 33 1 54 156 212 328 2.73e-31 121
MS.gene48800.t1 MTR_1g114540 79.070 86 1 1 147 232 414 482 1.40e-33 128
MS.gene48800.t1 MTR_1g114540 89.706 68 7 0 89 156 267 334 2.91e-32 124
MS.gene48800.t1 MTR_2g081070 80.882 68 12 1 89 155 281 348 2.33e-26 107
MS.gene48800.t1 MTR_2g081070 56.471 85 37 0 148 232 430 514 9.27e-24 99.8
MS.gene48800.t1 MTR_8g464480 70.588 68 19 1 89 155 262 329 5.31e-21 91.7
MS.gene48800.t1 MTR_8g464480 49.412 85 43 0 148 232 411 495 4.79e-20 89.0
MS.gene48800.t1 MTR_4g125690 63.492 63 18 1 89 146 153 215 2.38e-19 87.0
MS.gene48800.t1 MTR_7g115430 63.492 63 18 1 89 146 153 215 2.60e-19 87.0
MS.gene48800.t1 MTR_1g070280 63.492 63 18 1 89 146 153 215 5.31e-19 85.9
MS.gene48800.t1 MTR_1g070280 63.492 63 18 1 89 146 153 215 5.55e-19 85.9
MS.gene48800.t1 MTR_1g070280 63.492 63 18 1 89 146 153 215 6.08e-19 85.9
MS.gene48800.t1 MTR_7g099290 53.846 78 29 2 76 146 139 216 7.73e-19 85.5
MS.gene48800.t1 MTR_1g015620 60.317 63 20 1 89 146 150 212 8.34e-19 85.5
MS.gene48800.t1 MTR_1g080600 61.905 63 19 1 89 146 152 214 1.07e-18 85.1
MS.gene48800.t1 MTR_1g037500 60.317 63 20 1 89 146 153 215 1.86e-18 84.3
MS.gene48800.t1 MTR_1g070400 61.905 63 19 1 89 146 151 213 2.81e-18 84.0
MS.gene48800.t1 MTR_2g062660 52.778 72 29 1 80 146 144 215 3.10e-18 83.6
MS.gene48800.t1 MTR_3g105200 58.730 63 21 1 89 146 151 213 6.86e-18 82.8
MS.gene48800.t1 MTR_3g105200 58.730 63 21 1 89 146 151 213 6.99e-18 82.8
MS.gene48800.t1 MTR_8g010700 40.741 81 46 2 11 90 154 233 1.77e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48800.t1 AT2G37660 84.444 90 14 0 1 90 155 244 1.35e-52 173
MS.gene48800.t1 AT5G02240 83.333 90 15 0 1 90 83 172 7.01e-52 169
MS.gene48800.t1 AT1G50360 68.750 96 26 1 141 232 382 477 9.46e-37 137
MS.gene48800.t1 AT1G50360 86.765 68 9 0 89 156 245 312 1.04e-30 119
MS.gene48800.t1 AT3G19960 68.750 96 26 1 141 232 390 485 2.60e-36 135
MS.gene48800.t1 AT3G19960 85.507 69 10 0 89 157 253 321 7.46e-31 120
MS.gene48800.t1 AT3G19960 68.750 96 26 1 141 232 390 485 2.60e-36 135
MS.gene48800.t1 AT3G19960 85.507 69 10 0 89 157 253 321 7.46e-31 120
MS.gene48800.t1 AT3G19960 68.750 96 26 1 141 232 390 485 2.60e-36 135
MS.gene48800.t1 AT3G19960 85.507 69 10 0 89 157 253 321 7.46e-31 120
MS.gene48800.t1 AT3G19960 68.750 96 26 1 141 232 390 485 2.60e-36 135
MS.gene48800.t1 AT3G19960 85.507 69 10 0 89 157 253 321 8.51e-31 120
MS.gene48800.t1 AT1G42680 60.360 111 12 1 91 169 56 166 1.24e-33 119
MS.gene48800.t1 AT3G19960 56.410 117 26 2 141 232 406 522 5.18e-32 123
MS.gene48800.t1 AT3G19960 83.333 60 10 0 98 157 278 337 3.97e-25 103
MS.gene48800.t1 AT5G54280 53.125 96 41 1 141 232 439 534 6.59e-27 108
MS.gene48800.t1 AT5G54280 77.941 68 14 1 89 155 301 368 2.39e-24 101
MS.gene48800.t1 AT5G54280 53.125 96 41 1 141 232 249 344 7.83e-27 108
MS.gene48800.t1 AT5G54280 77.941 68 14 1 89 155 111 178 2.46e-24 101
MS.gene48800.t1 AT4G27370 79.412 68 13 1 89 155 254 321 2.99e-25 103
MS.gene48800.t1 AT4G27370 48.980 98 46 1 139 232 390 487 1.06e-24 102
MS.gene48800.t1 AT4G27370 79.412 68 13 1 89 155 252 319 3.02e-25 103
MS.gene48800.t1 AT4G27370 48.980 98 46 1 139 232 388 485 1.09e-24 102
MS.gene48800.t1 AT4G27370 79.412 68 13 1 89 155 254 321 3.17e-25 103
MS.gene48800.t1 AT4G27370 48.980 98 46 1 139 232 390 487 1.08e-24 102
MS.gene48800.t1 AT4G27370 79.412 68 13 1 89 155 254 321 3.17e-25 103
MS.gene48800.t1 AT4G27370 48.980 98 46 1 139 232 390 487 1.08e-24 102
MS.gene48800.t1 AT5G20490 65.079 63 17 1 89 146 87 149 7.64e-20 88.6
MS.gene48800.t1 AT5G20490 65.079 63 17 1 89 146 152 214 9.02e-20 88.2
MS.gene48800.t1 AT5G20490 65.079 63 17 1 89 146 153 215 9.02e-20 88.2
MS.gene48800.t1 AT1G17580 65.079 63 17 1 89 146 150 212 1.11e-19 88.2
MS.gene48800.t1 AT4G28710 55.844 77 28 2 76 146 138 214 1.61e-19 87.4
MS.gene48800.t1 AT1G04600 59.155 71 23 2 82 146 144 214 1.94e-19 87.4
MS.gene48800.t1 AT2G33240 59.155 71 23 2 82 146 166 236 1.98e-19 87.4
MS.gene48800.t1 AT2G33240 59.155 71 23 2 82 146 143 213 2.00e-19 87.4
MS.gene48800.t1 AT2G33240 59.155 71 23 2 82 146 161 231 2.06e-19 87.4
MS.gene48800.t1 AT2G33240 59.155 71 23 2 82 146 161 231 2.09e-19 87.4
MS.gene48800.t1 AT2G33240 59.155 71 23 2 82 146 161 231 2.09e-19 87.4
MS.gene48800.t1 AT2G33240 59.155 71 23 2 82 146 161 231 2.09e-19 87.4
MS.gene48800.t1 AT2G33240 59.155 71 23 2 82 146 161 231 2.10e-19 87.4
MS.gene48800.t1 AT5G43900 57.746 71 24 2 82 146 205 275 8.53e-19 85.5
MS.gene48800.t1 AT1G54560 61.905 63 19 1 89 146 156 218 8.92e-19 85.5
MS.gene48800.t1 AT1G54560 61.905 63 19 1 89 146 153 215 9.44e-19 85.5
MS.gene48800.t1 AT5G43900 57.746 71 24 2 82 146 145 215 9.79e-19 85.1
MS.gene48800.t1 AT1G08730 61.905 63 19 1 89 146 161 223 1.02e-18 85.1
MS.gene48800.t1 AT1G08730 61.905 63 19 1 89 146 153 215 1.07e-18 85.1
MS.gene48800.t1 AT4G33200 60.317 63 20 1 89 146 158 220 2.60e-18 84.0
MS.gene48800.t1 AT4G33200 60.317 63 20 1 89 146 158 220 2.76e-18 84.0
MS.gene48800.t1 AT4G33200 60.317 63 20 1 89 146 158 220 2.76e-18 84.0
MS.gene48800.t1 AT4G33200 60.317 63 20 1 89 146 158 220 2.97e-18 84.0
MS.gene48800.t1 AT2G31900 58.730 63 21 1 89 146 38 100 3.86e-18 83.6
MS.gene48800.t1 AT2G20290 56.338 71 25 2 82 146 155 225 4.22e-18 83.6
MS.gene48800.t1 AT2G20290 56.338 71 25 2 82 146 144 214 4.25e-18 83.6
MS.gene48800.t1 AT2G20290 56.338 71 25 2 82 146 144 214 4.26e-18 83.6
MS.gene48800.t1 AT2G20290 56.338 71 25 2 82 146 144 214 4.33e-18 83.2
MS.gene48800.t1 AT2G31900 58.730 63 21 1 89 146 153 215 4.53e-18 83.2
MS.gene48800.t1 AT2G31900 58.730 63 21 1 89 146 153 215 4.53e-18 83.2
MS.gene48800.t1 AT2G31900 58.730 63 21 1 89 146 153 215 4.53e-18 83.2
MS.gene48800.t1 AT1G04160 52.564 78 30 2 76 146 139 216 7.18e-18 82.8
MS.gene48800.t1 AT1G04160 52.564 78 30 2 76 146 139 216 7.75e-18 82.8
MS.gene48800.t1 AT3G58160 57.143 63 22 1 89 146 151 213 2.90e-17 80.9
MS.gene48800.t1 AT3G58160 57.143 63 22 1 89 146 151 213 3.07e-17 80.9
MS.gene48800.t1 AT3G58160 57.143 63 22 1 89 146 151 213 3.07e-17 80.9
MS.gene48800.t1 AT3G58160 57.143 63 22 1 89 146 151 213 3.23e-17 80.9
MS.gene48800.t1 AT3G58160 57.143 63 22 1 89 146 151 213 3.23e-17 80.9
MS.gene48800.t1 AT5G43900 58.824 68 25 2 82 146 205 272 1.13e-16 79.3

Find 59 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTGTTGGTTGGGTCTA+TGG 0.227152 4.4:-73588085 MS.gene48800:CDS
CCTGAGATAGCGTCAACTTT+TGG 0.282583 4.4:+73585935 None:intergenic
TAACCATCCTTTGAACAGTT+TGG 0.309070 4.4:-73588049 MS.gene48800:CDS
GAAGAATATCTGTCCAATTC+TGG 0.316073 4.4:-73587805 MS.gene48800:CDS
CAATGCTTGCTGCAGTATTA+TGG 0.317251 4.4:-73586612 MS.gene48800:CDS
AACCATCCTTTGAACAGTTT+GGG 0.323678 4.4:-73588048 MS.gene48800:CDS
CCTTTGAACAGTTTGGGAAA+TGG 0.335160 4.4:-73588042 MS.gene48800:CDS
TTACCAAAGGCTTCTAGTAT+TGG 0.351853 4.4:+73587024 None:intergenic
CGTGTATGGAACGCCAGAAT+TGG 0.367761 4.4:+73587792 None:intergenic
AAACTTAGGCATTCCACCTT+TGG 0.369028 4.4:+73588814 None:intergenic
TTCAGCACTGCCAAGTTGAT+TGG 0.374261 4.4:-73586027 MS.gene48800:CDS
GCTGCCCTTGGTGGTGGAAG+TGG 0.378989 4.4:-73587075 MS.gene48800:CDS
TATGATAGGTTGACTGGATT+GGG 0.379614 4.4:-73588671 MS.gene48800:intron
AAACTGTTCAAAGGATGGTT+AGG 0.380370 4.4:+73588051 None:intergenic
ATTGTGTTGGTTGGGTCTAT+GGG 0.382254 4.4:-73588084 MS.gene48800:CDS
CTTGGTCTCCTTTGCTAATG+TGG 0.384347 4.4:+73586647 None:intergenic
TAATAGTATTAGCACTTGTT+CGG 0.400129 4.4:+73588769 None:intergenic
TACCTGGCTGCCCTTGGTGG+TGG 0.421059 4.4:-73587081 MS.gene48800:CDS
AATAGTATTAGCACTTGTTC+GGG 0.427792 4.4:+73588770 None:intergenic
ATATGATAGGTTGACTGGAT+TGG 0.441585 4.4:-73588672 MS.gene48800:intron
TTTGGGAAATGGAAATATAC+TGG 0.444100 4.4:-73588031 MS.gene48800:intron
ATGCAATACCTGGCTGCCCT+TGG 0.444392 4.4:-73587087 MS.gene48800:CDS
ATACTAGAAGCCTTTGGTAA+TGG 0.447037 4.4:-73587021 MS.gene48800:CDS
CAATACCTGGCTGCCCTTGG+TGG 0.463295 4.4:-73587084 MS.gene48800:CDS
CCAAAAGTTGACGCTATCTC+AGG 0.470094 4.4:-73585935 MS.gene48800:CDS
TATAGTGGTGAGAGTGGAGC+AGG 0.471356 4.4:-73587135 MS.gene48800:intron
TGAAGCATATTGTGTTGGTT+GGG 0.478830 4.4:-73588092 MS.gene48800:CDS
GCATTGCAAATACATTCTCT+TGG 0.493269 4.4:+73586629 None:intergenic
AATCCAATACTAGAAGCCTT+TGG 0.493384 4.4:-73587027 MS.gene48800:CDS
TTCCCAAACTGTTCAAAGGA+TGG 0.501526 4.4:+73588046 None:intergenic
AACTGTTCAAAGGATGGTTA+GGG 0.504635 4.4:+73588052 None:intergenic
CCATTTCCCAAACTGTTCAA+AGG 0.506648 4.4:+73588042 None:intergenic
GTGTACCTTATGATCGTGTA+TGG 0.507273 4.4:+73587778 None:intergenic
CTATGAACAGCTAAGGCTGC+AGG 0.510205 4.4:-73588117 MS.gene48800:intron
TCAACTCGCAAAATGAAAGT+TGG 0.513663 4.4:-73585973 MS.gene48800:CDS
GTGAAGCATATTGTGTTGGT+TGG 0.518358 4.4:-73588093 MS.gene48800:CDS
GCGTTCCATACACGATCATA+AGG 0.520759 4.4:-73587783 MS.gene48800:intron
TGTTCAAGCTGTAGAGGATG+AGG 0.521445 4.4:-73586548 MS.gene48800:CDS
TTTATAACATAAATGAAACC+TGG 0.522348 4.4:-73588848 None:intergenic
TTAGGCATTCCACCTTTGGT+TGG 0.527865 4.4:+73588818 None:intergenic
GTGTTGGTTGGGTCTATGGG+TGG 0.540126 4.4:-73588081 MS.gene48800:CDS
AATGTCACAGCCAATCAACT+TGG 0.541437 4.4:+73586017 None:intergenic
TTCCACCACCAAGGGCAGCC+AGG 0.542075 4.4:+73587079 None:intergenic
CTTGCTGCAGTATTATGGTT+GGG 0.550850 4.4:-73586607 MS.gene48800:CDS
TTATAACATAAATGAAACCT+GGG 0.564873 4.4:-73588847 None:intergenic
CCTTTGGTTGGATCAAACCC+AGG 0.573204 4.4:+73588830 None:intergenic
GCTTGCTGCAGTATTATGGT+TGG 0.579317 4.4:-73586608 MS.gene48800:CDS
ATAGTGGTGAGAGTGGAGCA+GGG 0.585236 4.4:-73587134 MS.gene48800:intron
AAATCATGTTCAAGCTGTAG+AGG 0.586742 4.4:-73586554 MS.gene48800:CDS
GGGTTTGATCCAACCAAAGG+TGG 0.589696 4.4:-73588827 MS.gene48800:CDS
CCATCATCAAAATAAAACTT+AGG 0.599800 4.4:+73588800 None:intergenic
TACATGTATAGTGGTGAGAG+TGG 0.600129 4.4:-73587141 MS.gene48800:intron
AAGGGCAGCCAGGTATTGCA+TGG 0.617488 4.4:+73587089 None:intergenic
TATTCCACTTCCACCACCAA+GGG 0.629576 4.4:+73587071 None:intergenic
GATGTTGTCCACATTAGCAA+AGG 0.633529 4.4:-73586655 MS.gene48800:CDS
AGGAGTGAAGCATATTGTGT+TGG 0.636269 4.4:-73588097 MS.gene48800:CDS
CTATTCCACTTCCACCACCA+AGG 0.644612 4.4:+73587070 None:intergenic
CCTGGGTTTGATCCAACCAA+AGG 0.647766 4.4:-73588830 MS.gene48800:CDS
GTTCAAGCTGTAGAGGATGA+GGG 0.655939 4.4:-73586547 MS.gene48800:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAGAATAAGAATTATTAT+AGG + chr4.4:73587598-73587617 None:intergenic 10.0%
!!! AACTTATATATGTTTTAACA+TGG + chr4.4:73587021-73587040 None:intergenic 15.0%
!!! TAGTTAAAAGTATTTTAAGT+AGG - chr4.4:73587334-73587353 MS.gene48800:intron 15.0%
!!! TGTAAAAGTATTTTAAGTTT+AGG - chr4.4:73587420-73587439 MS.gene48800:intron 15.0%
!! AAAAATGCATAAAATGTGTT+TGG - chr4.4:73586223-73586242 MS.gene48800:intron 20.0%
!! AACTTATACCTCAATTAAAA+AGG - chr4.4:73588683-73588702 MS.gene48800:intron 20.0%
!! AAGTAAGAATCAATACATAT+AGG + chr4.4:73587817-73587836 None:intergenic 20.0%
!! AGATATAACAGAAAGAATAA+AGG + chr4.4:73586883-73586902 None:intergenic 20.0%
!! ATCAATCCAGTTTATTATTT+AGG + chr4.4:73587225-73587244 None:intergenic 20.0%
!! GGAAATATATGAAATATGTA+GGG - chr4.4:73587376-73587395 MS.gene48800:intron 20.0%
!! TAATTTGATTGAACTTTACA+GGG - chr4.4:73587890-73587909 MS.gene48800:intron 20.0%
!!! AAACGAAATAACAAAAGTAT+TGG + chr4.4:73586773-73586792 None:intergenic 20.0%
!!! AACACATTTTATGCATTTTT+AGG + chr4.4:73586223-73586242 None:intergenic 20.0%
!!! AATGAAAACTGAATGTTTTT+AGG - chr4.4:73586513-73586532 MS.gene48800:intron 20.0%
!!! ACAAAATTCCTTTTTAATTG+AGG + chr4.4:73588694-73588713 None:intergenic 20.0%
!!! TATATGTATTGATTCTTACT+TGG - chr4.4:73587816-73587835 MS.gene48800:CDS 20.0%
!!! TTAATTTGATTGAACTTTAC+AGG - chr4.4:73587889-73587908 MS.gene48800:intron 20.0%
!!! TTCATTTTAAATTGCTAACA+AGG + chr4.4:73586499-73586518 None:intergenic 20.0%
! AAATCCATATAATAAACGCT+TGG + chr4.4:73587196-73587215 None:intergenic 25.0%
! AACCTATCATATGAAAAAGT+AGG + chr4.4:73586083-73586102 None:intergenic 25.0%
! AATCCAGTTTATTATTTAGG+AGG + chr4.4:73587222-73587241 None:intergenic 25.0%
! ATATAATAAACGCTTGGATA+TGG + chr4.4:73587190-73587209 None:intergenic 25.0%
! ATATCCAAGCGTTTATTATA+TGG - chr4.4:73587189-73587208 MS.gene48800:intron 25.0%
! CATATAAAATACTTATGCAG+CGG + chr4.4:73588644-73588663 None:intergenic 25.0%
! CATATGAACGAAAAATAAAG+AGG + chr4.4:73588487-73588506 None:intergenic 25.0%
! CCATCATCAAAATAAAACTT+AGG + chr4.4:73585966-73585985 None:intergenic 25.0%
! CTTATAATCTACATGTATAG+TGG - chr4.4:73587613-73587632 MS.gene48800:intron 25.0%
! GAAAACAAAACTGAAAAGAT+AGG + chr4.4:73587047-73587066 None:intergenic 25.0%
! GAATCAATACATATAGGATT+AGG + chr4.4:73587811-73587830 None:intergenic 25.0%
! GATCGTTATATTATTGTGTT+TGG + chr4.4:73586359-73586378 None:intergenic 25.0%
! GGGAAATATATGAAATATGT+AGG - chr4.4:73587375-73587394 MS.gene48800:intron 25.0%
! TAAGTTTAGGAATGAATGAT+CGG - chr4.4:73587433-73587452 MS.gene48800:intron 25.0%
! TAATAAAAAACCTCAAGAAC+AGG + chr4.4:73587842-73587861 None:intergenic 25.0%
! TAATAGTATTAGCACTTGTT+CGG + chr4.4:73585997-73586016 None:intergenic 25.0%
! TATCTTAGAGAATGTTTACT+TGG - chr4.4:73586267-73586286 MS.gene48800:intron 25.0%
! TATGTAGGGAAATATGAAAA+AGG - chr4.4:73587390-73587409 MS.gene48800:intron 25.0%
! TGATATTAATGAGAATCAAC+AGG + chr4.4:73587515-73587534 None:intergenic 25.0%
! TTGTTCCATTACTTCTTAAA+GGG - chr4.4:73588710-73588729 MS.gene48800:intron 25.0%
! TTTACCAGAAATCTTTAGTT+CGG + chr4.4:73586560-73586579 None:intergenic 25.0%
! TTTCTTGTAAATTAGTGAAG+AGG - chr4.4:73588410-73588429 MS.gene48800:intron 25.0%
! TTTGTTCCATTACTTCTTAA+AGG - chr4.4:73588709-73588728 MS.gene48800:intron 25.0%
!! ATTTTAAATTGCTAACAAGG+TGG + chr4.4:73586496-73586515 None:intergenic 25.0%
!!! AATGTTTTTAGGAACTAAAC+AGG - chr4.4:73586524-73586543 MS.gene48800:intron 25.0%
!!! AGGCTTTTGTAAAAGTATTT+AGG - chr4.4:73587354-73587373 MS.gene48800:intron 25.0%
!!! ATGTTTTTAGGAACTAAACA+GGG - chr4.4:73586525-73586544 MS.gene48800:intron 25.0%
!!! ATTATTTCTGCTTTGTGTAA+TGG - chr4.4:73588045-73588064 MS.gene48800:CDS 25.0%
!!! CCTAAGTTTTATTTTGATGA+TGG - chr4.4:73585963-73585982 MS.gene48800:CDS 25.0%
!!! CTCTTTATTTTTCGTTCATA+TGG - chr4.4:73588485-73588504 MS.gene48800:intron 25.0%
!!! GAAAAAGTTGCAGTTTTTTA+AGG - chr4.4:73588458-73588477 MS.gene48800:intron 25.0%
!!! GGCTTTTGTAAAAGTATTTA+GGG - chr4.4:73587355-73587374 MS.gene48800:intron 25.0%
AATAGTATTAGCACTTGTTC+GGG + chr4.4:73585996-73586015 None:intergenic 30.0%
ACATATGCAAGCAATAGTTA+TGG + chr4.4:73588602-73588621 None:intergenic 30.0%
AGTGAATAATGACTTTGGTT+AGG + chr4.4:73586596-73586615 None:intergenic 30.0%
ATAACTATTGCTTGCATATG+TGG - chr4.4:73588601-73588620 MS.gene48800:intron 30.0%
CAGAAAAATCAAATAGATGC+TGG - chr4.4:73586115-73586134 MS.gene48800:intron 30.0%
CTTACATCATAACTTCACAA+GGG + chr4.4:73588539-73588558 None:intergenic 30.0%
TCTTACATCATAACTTCACA+AGG + chr4.4:73588540-73588559 None:intergenic 30.0%
TGTAAATTAGTGAAGAGGAA+AGG - chr4.4:73588415-73588434 MS.gene48800:intron 30.0%
TTCTATCCATTACTCATATG+CGG - chr4.4:73586152-73586171 MS.gene48800:intron 30.0%
TTGAGGACTATAAATATCTG+AGG - chr4.4:73587936-73587955 MS.gene48800:intron 30.0%
TTGCCTCCTAAATAATAAAC+TGG - chr4.4:73587216-73587235 MS.gene48800:intron 30.0%
TTGTGAACAATTGCGATAAA+GGG + chr4.4:73586463-73586482 None:intergenic 30.0%
TTTATCTCTATGAACAGCTA+AGG - chr4.4:73586639-73586658 MS.gene48800:CDS 30.0%
TTTGGGAAATGGAAATATAC+TGG - chr4.4:73586732-73586751 MS.gene48800:intron 30.0%
! CAATGTTTTTCCATTACCAA+AGG + chr4.4:73587755-73587774 None:intergenic 30.0%
! CACATGAACTTTTTAATACC+AGG - chr4.4:73588077-73588096 MS.gene48800:CDS 30.0%
!! TTTTCATATGATAGGTTGAC+TGG - chr4.4:73586086-73586105 MS.gene48800:intron 30.0%
!!! GACCTACTTTTTCATATGAT+AGG - chr4.4:73586078-73586097 MS.gene48800:intron 30.0%
AAAAATAGCCATGCAATACC+TGG - chr4.4:73587666-73587685 MS.gene48800:intron 35.0%
AAACTGTTCAAAGGATGGTT+AGG + chr4.4:73586715-73586734 None:intergenic 35.0%
AAATCATGTTCAAGCTGTAG+AGG - chr4.4:73588209-73588228 MS.gene48800:intron 35.0%
AACCATCCTTTGAACAGTTT+GGG - chr4.4:73586715-73586734 MS.gene48800:intron 35.0%
AACTGTTCAAAGGATGGTTA+GGG + chr4.4:73586714-73586733 None:intergenic 35.0%
AATCCAATACTAGAAGCCTT+TGG - chr4.4:73587736-73587755 MS.gene48800:intron 35.0%
AATGAATGATCGGTTAACAG+CGG - chr4.4:73587443-73587462 MS.gene48800:intron 35.0%
ATACTAGAAGCCTTTGGTAA+TGG - chr4.4:73587742-73587761 MS.gene48800:intron 35.0%
ATTTCCAGAATCTCTACTGA+TGG - chr4.4:73588244-73588263 MS.gene48800:intron 35.0%
CATTGAGATTCACTTCATTG+AGG - chr4.4:73587919-73587938 MS.gene48800:intron 35.0%
CCCTTAAAAACATTCCGAAT+GGG + chr4.4:73586313-73586332 None:intergenic 35.0%
CCTTAAAAACATTCCGAATG+GGG + chr4.4:73586312-73586331 None:intergenic 35.0%
CTTAAAAACATTCCGAATGG+GGG + chr4.4:73586311-73586330 None:intergenic 35.0%
GAAGAATATCTGTCCAATTC+TGG - chr4.4:73586958-73586977 MS.gene48800:intron 35.0%
GATTCCGAACTAAAGATTTC+TGG - chr4.4:73586553-73586572 MS.gene48800:CDS 35.0%
GCAGAAAGTGAAAAAGTCTA+AGG + chr4.4:73587554-73587573 None:intergenic 35.0%
GCATGAGTGAATAATGACTT+TGG + chr4.4:73586601-73586620 None:intergenic 35.0%
GCATTGCAAATACATTCTCT+TGG + chr4.4:73588137-73588156 None:intergenic 35.0%
GCTAAAAACTATTCCATCGT+AGG + chr4.4:73588370-73588389 None:intergenic 35.0%
GGAATGTGTAGAATACTTGT+GGG - chr4.4:73586244-73586263 MS.gene48800:intron 35.0%
GGTTCAGCTATGATTACTAA+AGG + chr4.4:73587148-73587167 None:intergenic 35.0%
GTTGTGAACAATTGCGATAA+AGG + chr4.4:73586464-73586483 None:intergenic 35.0%
TAACCATCCTTTGAACAGTT+TGG - chr4.4:73586714-73586733 MS.gene48800:intron 35.0%
TCAACTCGCAAAATGAAAGT+TGG - chr4.4:73588790-73588809 MS.gene48800:CDS 35.0%
TCATTTAGCATATCCTACGA+TGG - chr4.4:73588354-73588373 MS.gene48800:intron 35.0%
TGGAATGTGTAGAATACTTG+TGG - chr4.4:73586243-73586262 MS.gene48800:intron 35.0%
TGGATTGATGCTTTGTAGAT+TGG - chr4.4:73587236-73587255 MS.gene48800:intron 35.0%
TTACCAAAGGCTTCTAGTAT+TGG + chr4.4:73587742-73587761 None:intergenic 35.0%
! AACAGCCCTTTAAGAAGTAA+TGG + chr4.4:73588718-73588737 None:intergenic 35.0%
! AGCTTTTGCATAACAAGTTG+TGG + chr4.4:73588290-73588309 None:intergenic 35.0%
! ATATGATAGGTTGACTGGAT+TGG - chr4.4:73586091-73586110 MS.gene48800:intron 35.0%
! TATGATAGGTTGACTGGATT+GGG - chr4.4:73586092-73586111 MS.gene48800:intron 35.0%
! TTTTTAATACCAGGATGCTC+TGG - chr4.4:73588086-73588105 MS.gene48800:CDS 35.0%
!! CCATTCGGAATGTTTTTAAG+GGG - chr4.4:73586311-73586330 MS.gene48800:intron 35.0%
!! CCCATTCGGAATGTTTTTAA+GGG - chr4.4:73586310-73586329 MS.gene48800:intron 35.0%
!! GTTTTAACATGGACAAGAAC+TGG + chr4.4:73587010-73587029 None:intergenic 35.0%
!! TGAAGCATATTGTGTTGGTT+GGG - chr4.4:73586671-73586690 MS.gene48800:intron 35.0%
!!! AGACTTTTTCACTTTCTGCT+TGG - chr4.4:73587555-73587574 MS.gene48800:intron 35.0%
!!! GAGTGTTTTGACAGAAAACA+TGG + chr4.4:73586392-73586411 None:intergenic 35.0%
AAAAGCTCTGTCTAGTGCTT+TGG - chr4.4:73588303-73588322 MS.gene48800:intron 40.0%
AAACTTAGGCATTCCACCTT+TGG + chr4.4:73585952-73585971 None:intergenic 40.0%
AAAGCTCTGTCTAGTGCTTT+GGG - chr4.4:73588304-73588323 MS.gene48800:intron 40.0%
AATGTCACAGCCAATCAACT+TGG + chr4.4:73588749-73588768 None:intergenic 40.0%
AGGAGTGAAGCATATTGTGT+TGG - chr4.4:73586666-73586685 MS.gene48800:CDS 40.0%
CAATTGCGATAAAGGGAAGA+TGG + chr4.4:73586456-73586475 None:intergenic 40.0%
CCATTTCCCAAACTGTTCAA+AGG + chr4.4:73586724-73586743 None:intergenic 40.0%
CCCCTTAAAAACATTCCGAA+TGG + chr4.4:73586314-73586333 None:intergenic 40.0%
CCTTTGAACAGTTTGGGAAA+TGG - chr4.4:73586721-73586740 MS.gene48800:intron 40.0%
CTGAACCAATACGACAATTG+CGG - chr4.4:73587161-73587180 MS.gene48800:intron 40.0%
CTTGCTGCAGTATTATGGTT+GGG - chr4.4:73588156-73588175 MS.gene48800:intron 40.0%
CTTTGTAGATTGGTGAGGAT+TGG - chr4.4:73587246-73587265 MS.gene48800:intron 40.0%
GATGTTGTCCACATTAGCAA+AGG - chr4.4:73588108-73588127 MS.gene48800:CDS 40.0%
GATTCCATCAGTAGAGATTC+TGG + chr4.4:73588251-73588270 None:intergenic 40.0%
GTGTACCTTATGATCGTGTA+TGG + chr4.4:73586988-73587007 None:intergenic 40.0%
TAAAGGGAAGATGGAAAGCA+AGG + chr4.4:73586447-73586466 None:intergenic 40.0%
TACATGTATAGTGGTGAGAG+TGG - chr4.4:73587622-73587641 MS.gene48800:intron 40.0%
TCAATCTTCTTACCCCCATT+CGG - chr4.4:73586296-73586315 MS.gene48800:intron 40.0%
TGATGCTTTGTAGATTGGTG+AGG - chr4.4:73587241-73587260 MS.gene48800:intron 40.0%
TGCTTGGCTGTATTTGAACT+GGG - chr4.4:73587571-73587590 MS.gene48800:intron 40.0%
TTCCCAAACTGTTCAAAGGA+TGG + chr4.4:73586720-73586739 None:intergenic 40.0%
TTCTGCTTTGTGTAATGGCT+CGG - chr4.4:73588050-73588069 MS.gene48800:CDS 40.0%
! AGAAGCTTTAACCACTCAAG+CGG + chr4.4:73587484-73587503 None:intergenic 40.0%
! CAATGCTTGCTGCAGTATTA+TGG - chr4.4:73588151-73588170 MS.gene48800:intron 40.0%
! GTGAAGCATATTGTGTTGGT+TGG - chr4.4:73586670-73586689 MS.gene48800:intron 40.0%
! TTTTGATCAGTGCAATGCAC+AGG + chr4.4:73587098-73587117 None:intergenic 40.0%
!! ATTGTGTTGGTTGGGTCTAT+GGG - chr4.4:73586679-73586698 MS.gene48800:intron 40.0%
!! CCCCATTCGGAATGTTTTTA+AGG - chr4.4:73586309-73586328 MS.gene48800:intron 40.0%
!! TATTGTGTTGGTTGGGTCTA+TGG - chr4.4:73586678-73586697 MS.gene48800:intron 40.0%
AGAACAAGTTACCGCTTGAG+TGG - chr4.4:73587470-73587489 MS.gene48800:intron 45.0%
CAAACGCCGCATATGAGTAA+TGG + chr4.4:73586161-73586180 None:intergenic 45.0%
CATTACTCATATGCGGCGTT+TGG - chr4.4:73586159-73586178 MS.gene48800:intron 45.0%
CCAAAAGTTGACGCTATCTC+AGG - chr4.4:73588828-73588847 MS.gene48800:CDS 45.0%
CTGCTTGGCTGTATTTGAAC+TGG - chr4.4:73587570-73587589 MS.gene48800:intron 45.0%
CTTGGTCTCCTTTGCTAATG+TGG + chr4.4:73588119-73588138 None:intergenic 45.0%
GCGTTCCATACACGATCATA+AGG - chr4.4:73586980-73586999 MS.gene48800:intron 45.0%
GTAGATTGGTGAGGATTGGA+AGG - chr4.4:73587250-73587269 MS.gene48800:intron 45.0%
GTTCAAGCTGTAGAGGATGA+GGG - chr4.4:73588216-73588235 MS.gene48800:intron 45.0%
TATTCCACTTCCACCACCAA+GGG + chr4.4:73587695-73587714 None:intergenic 45.0%
TCTTACTTGGCCTGTTCTTG+AGG - chr4.4:73587829-73587848 MS.gene48800:intron 45.0%
TGTTCAAGCTGTAGAGGATG+AGG - chr4.4:73588215-73588234 MS.gene48800:intron 45.0%
TTAGGCATTCCACCTTTGGT+TGG + chr4.4:73585948-73585967 None:intergenic 45.0%
! CCTGAGATAGCGTCAACTTT+TGG + chr4.4:73588831-73588850 None:intergenic 45.0%
! GCTTGCTGCAGTATTATGGT+TGG - chr4.4:73588155-73588174 MS.gene48800:intron 45.0%
! TGTTTTCACAGTCCCAAACC+TGG - chr4.4:73587060-73587079 MS.gene48800:CDS 45.0%
!! TTCAGCACTGCCAAGTTGAT+TGG - chr4.4:73588736-73588755 MS.gene48800:intron 45.0%
ATAGTGGTGAGAGTGGAGCA+GGG - chr4.4:73587629-73587648 MS.gene48800:intron 50.0%
CCTGGGTTTGATCCAACCAA+AGG - chr4.4:73585933-73585952 MS.gene48800:CDS 50.0%
CCTTTGGTTGGATCAAACCC+AGG + chr4.4:73585936-73585955 None:intergenic 50.0%
CGTGTATGGAACGCCAGAAT+TGG + chr4.4:73586974-73586993 None:intergenic 50.0%
CTATGAACAGCTAAGGCTGC+AGG - chr4.4:73586646-73586665 MS.gene48800:CDS 50.0%
CTATTCCACTTCCACCACCA+AGG + chr4.4:73587696-73587715 None:intergenic 50.0%
GAGGATTGGAAGGAACTGGT+GGG - chr4.4:73587260-73587279 MS.gene48800:intron 50.0%
GATCAGTGCAATGCACAGGT+AGG + chr4.4:73587094-73587113 None:intergenic 50.0%
GGACACCGCAATTGTCGTAT+TGG + chr4.4:73587169-73587188 None:intergenic 50.0%
GGGAAGATGGAAAGCAAGGT+AGG + chr4.4:73586443-73586462 None:intergenic 50.0%
GGGTTTGATCCAACCAAAGG+TGG - chr4.4:73585936-73585955 MS.gene48800:CDS 50.0%
TAGGTGGTCTGTCCAGGTTT+GGG + chr4.4:73587075-73587094 None:intergenic 50.0%
TATAGTGGTGAGAGTGGAGC+AGG - chr4.4:73587628-73587647 MS.gene48800:intron 50.0%
TGAGGATTGGAAGGAACTGG+TGG - chr4.4:73587259-73587278 MS.gene48800:intron 50.0%
TGGTGAGGATTGGAAGGAAC+TGG - chr4.4:73587256-73587275 MS.gene48800:intron 50.0%
! TCTGTCTAGTGCTTTGGGCT+TGG - chr4.4:73588309-73588328 MS.gene48800:intron 50.0%
ATGCAATACCTGGCTGCCCT+TGG - chr4.4:73587676-73587695 MS.gene48800:intron 55.0%
CAGTGCAATGCACAGGTAGG+TGG + chr4.4:73587091-73587110 None:intergenic 55.0%
GTAGGTGGTCTGTCCAGGTT+TGG + chr4.4:73587076-73587095 None:intergenic 55.0%
! AAGGGCAGCCAGGTATTGCA+TGG + chr4.4:73587677-73587696 None:intergenic 55.0%
! GGACAACATCCAGAGCATCC+TGG + chr4.4:73588098-73588117 None:intergenic 55.0%
!! GTGTTGGTTGGGTCTATGGG+TGG - chr4.4:73586682-73586701 MS.gene48800:intron 55.0%
CAATACCTGGCTGCCCTTGG+TGG - chr4.4:73587679-73587698 MS.gene48800:intron 60.0%
CACAGGTAGGTGGTCTGTCC+AGG + chr4.4:73587081-73587100 None:intergenic 60.0%
TACCTGGCTGCCCTTGGTGG+TGG - chr4.4:73587682-73587701 MS.gene48800:intron 65.0%
TTCCACCACCAAGGGCAGCC+AGG + chr4.4:73587687-73587706 None:intergenic 65.0%
!! GCTGCCCTTGGTGGTGGAAG+TGG - chr4.4:73587688-73587707 MS.gene48800:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 73585927 73588858 73585927 ID=MS.gene48800
chr4.4 mRNA 73585927 73588858 73585927 ID=MS.gene48800.t1;Parent=MS.gene48800
chr4.4 exon 73588784 73588858 73588784 ID=MS.gene48800.t1.exon1;Parent=MS.gene48800.t1
chr4.4 CDS 73588784 73588858 73588784 ID=cds.MS.gene48800.t1;Parent=MS.gene48800.t1
chr4.4 exon 73588649 73588685 73588649 ID=MS.gene48800.t1.exon2;Parent=MS.gene48800.t1
chr4.4 CDS 73588649 73588685 73588649 ID=cds.MS.gene48800.t1;Parent=MS.gene48800.t1
chr4.4 exon 73588032 73588129 73588032 ID=MS.gene48800.t1.exon3;Parent=MS.gene48800.t1
chr4.4 CDS 73588032 73588129 73588032 ID=cds.MS.gene48800.t1;Parent=MS.gene48800.t1
chr4.4 exon 73587784 73587845 73587784 ID=MS.gene48800.t1.exon4;Parent=MS.gene48800.t1
chr4.4 CDS 73587784 73587845 73587784 ID=cds.MS.gene48800.t1;Parent=MS.gene48800.t1
chr4.4 exon 73586987 73587152 73586987 ID=MS.gene48800.t1.exon5;Parent=MS.gene48800.t1
chr4.4 CDS 73586987 73587152 73586987 ID=cds.MS.gene48800.t1;Parent=MS.gene48800.t1
chr4.4 exon 73586548 73586686 73586548 ID=MS.gene48800.t1.exon6;Parent=MS.gene48800.t1
chr4.4 CDS 73586548 73586686 73586548 ID=cds.MS.gene48800.t1;Parent=MS.gene48800.t1
chr4.4 exon 73585927 73586054 73585927 ID=MS.gene48800.t1.exon7;Parent=MS.gene48800.t1
chr4.4 CDS 73585927 73586054 73585927 ID=cds.MS.gene48800.t1;Parent=MS.gene48800.t1
Gene Sequence

>MS.gene48800

ATGAAACCTGGGTTTGATCCAACCAAAGGTGGAATGCCTAAGTTTTATTTTGATGATGGTGCATATCCCGAACAAGTGCTAATACTATTACATTTTTCATATGCATGCTAAGAATAATTTTGAATATAATGTCTCATTTGACTTAAATTTTGACCTACTTTTTCATATGATAGGTTGACTGGATTGGGCAGAAAAATCAAATAGATGCTGGTAAGAGTCAATGCTTTCTATCCATTACTCATATGCGGCGTTTGGTATGCAGAGTTTTTTTTTCTTTCTTTTGAGTCTTGATTCCTAAAAATGCATAAAATGTGTTTGGAATGTGTAGAATACTTGTGGGTATCTTAGAGAATGTTTACTTGGATGTTTTCAATCTTCTTACCCCCATTCGGAATGTTTTTAAGGGGAACAAAATTGAAAAAAATCACACCAAACACAATAATATAACGATCTGTTTACTTACCATGTTTTCTGTCAAAACACTCTTTTCATTAATTCTGAAAACACATTCTTCCTACCTTGCTTTCCATCTTCCCTTTATCGCAATTGTTCACAACGTAAGTCTTCCACCTTGTTAGCAATTTAAAATGAAAACTGAATGTTTTTAGGAACTAAACAGGGTCTAAGATTCCGAACTAAAGATTTCTGGTAAACTTTAAAAAAATTCCTAACCAAAGTCATTATTCACTCATGCTTATGATATGAAAATTATTTTATCTCTATGAACAGCTAAGGCTGCAGGAGTGAAGCATATTGTGTTGGTTGGGTCTATGGGTGGAACAAACCCTAACCATCCTTTGAACAGTTTGGGAAATGGAAATATACTGGTGAGTTGTATTCTCTCCAATACTTTTGTTATTTCGTTTCATAGTCATAACATTTTCATGTTGCTTTTATGTTTGAACATATCTCACATTTTATTTCTAAATAGAATAGAACACATTTTTTTTCATCCTTTATTCTTTCTGTTATATCTTTTGATAGAAATCATATAGTTTCATTATTAATTGCAGCTTTTGAAAAGAAAAGCTGAAGAATATCTGTCCAATTCTGGCGTTCCATACACGATCATAAGGTACACCAGTTCTTGTCCATGTTAAAACATATATAAGTTATACCTATCTTTTCAGTTTTGTTTTCACAGTCCCAAACCTGGACAGACCACCTACCTGTGCATTGCACTGATCAAAAACACTCGTTACGAACTTACGACTAATGCCTTTAGTAATCATAGCTGAACCAATACGACAATTGCGGTGTCCATATCCAAGCGTTTATTATATGGATTTTTGCCTCCTAAATAATAAACTGGATTGATGCTTTGTAGATTGGTGAGGATTGGAAGGAACTGGTGGGAAACTAATATGTATTATTTGATGCATTACTACTAGCATTAGCAGCAGTTATTAGTTAAAAGTATTTTAAGTAGGCTTTTGTAAAAGTATTTAGGGAAATATATGAAATATGTAGGGAAATATGAAAAAGGCATTATTTGTAAAAGTATTTTAAGTTTAGGAATGAATGATCGGTTAACAGCGGTCAAAGAACAAGTTACCGCTTGAGTGGTTAAAGCTTCTTTATGCTGCCTGTTGATTCTCATTAATATCAAGCTCAAATTGATTTTCCTTAGACTTTTTCACTTTCTGCTTGGCTGTATTTGAACTGGGACCTATAATAATTCTTATTCTTATAATCTACATGTATAGTGGTGAGAGTGGAGCAGGGAAGACTGAGACAGCAAAAATAGCCATGCAATACCTGGCTGCCCTTGGTGGTGGAAGTGGAATAGAGCATGAAATATTAAAGACTAATCCAATACTAGAAGCCTTTGGTAATGGAAAAACATTGAGAAATGACAATTCAAGTCGTTTTGTAAGATTTCCTAATCCTATATGTATTGATTCTTACTTGGCCTGTTCTTGAGGTTTTTTATTATACTCTCTTTTGATGCTGAAATCTCTCTTAATTTGATTGAACTTTACAGGGAAAGCTCATTGAGATTCACTTCATTGAGGACTATAAATATCTGAGGCAGAGCAATTGTTATTCAATTAATGTTGTTGATGTTGCTTACTGATGATCTGAATCAATATAGTTAATATTGTATGATTTTTTTTAATTATTTCTGCTTTGTGTAATGGCTCGGCATACACATGAACTTTTTAATACCAGGATGCTCTGGATGTTGTCCACATTAGCAAAGGAGACCAAGAGAATGTATTTGCAATGCTTGCTGCAGTATTATGGTTGGGAAACATATCATTTACTGTGATCGATAATGAAAATCATGTTCAAGCTGTAGAGGATGAGGGTAAGAATTTCCAGAATCTCTACTGATGGAATCTAAAGCATAGTTTTTTCCACAACTTGTTATGCAAAAGCTCTGTCTAGTGCTTTGGGCTTGGTATGTCTTTGAATATTTTGCTTTCATTTAGCATATCCTACGATGGAATAGTTTTTAGCATTTTATATTTTTCAAAAAATTTCTTGTAAATTAGTGAAGAGGAAAGGTAATGAGTTTTACATTTTGAGAAAAAGTTGCAGTTTTTTAAGGTTTCCTCTTTATTTTTCGTTCATATGGTTTTTGATTGATAATCATTGATTTACTGCCCTTGTGAAGTTATGATGTAAGAATCATGTAATAACTTGAGCATGTTTGCATTTTATGATGACCATAACTATTGCTTGCATATGTGGCACATGCTACAGAATTACCGCTGCATAAGTATTTTATATGCTTTCATGCTTTATAATTTAACTTATACCTCAATTAAAAAGGAATTTTGTTCCATTACTTCTTAAAGGGCTGTTCAGCACTGCCAAGTTGATTGGCTGTGACATTGAAGATCTGAAGTTGACTCTATCAACTCGCAAAATGAAAGTTGGTAAAGATACTATTGTCCAAAAGTTGACGCTATCTCAGGTAAAATGA
Protein sequence

>MS.gene48800.t1

MKPGFDPTKGGMPKFYFDDGAYPEQVDWIGQKNQIDAAKAAGVKHIVLVGSMGGTNPNHPLNSLGNGNILLLKRKAEEYLSNSGVPYTIISGESGAGKTETAKIAMQYLAALGGGSGIEHEILKTNPILEAFGNGKTLRNDNSSRFDALDVVHISKGDQENVFAMLAAVLWLGNISFTVIDNENHVQAVEDEGLFSTAKLIGCDIEDLKLTLSTRKMKVGKDTIVQKLTLSQVK