Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48875.t1 | KEH29169.1 | 95.3 | 232 | 11 | 0 | 1 | 232 | 1 | 232 | 5.30E-118 | 433.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48875.t1 | A0A072UHD2 | 95.3 | 232 | 11 | 0 | 1 | 232 | 1 | 232 | 3.8e-118 | 433.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059265 | MS.gene48875 | 0.871969 | 4.56E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48875.t1 | MTR_4g027195 | 95.259 | 232 | 11 | 0 | 1 | 232 | 1 | 232 | 8.20e-157 | 434 |
| MS.gene48875.t1 | MTR_4g027105 | 68.421 | 171 | 48 | 3 | 60 | 228 | 25 | 191 | 2.98e-71 | 216 |
| MS.gene48875.t1 | MTR_4g027225 | 66.667 | 171 | 51 | 3 | 60 | 228 | 25 | 191 | 1.93e-59 | 186 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTTGCCCTTCTTCTCTT+TGG | 0.204053 | 4.3:-71894840 | MS.gene48875:CDS |
| TCAACTAGGTCAGCTGGATT+TGG | 0.237066 | 4.3:+71894600 | None:intergenic |
| TGACCATATTGTATTGGATT+TGG | 0.293386 | 4.3:-71895738 | MS.gene48875:CDS |
| TCGTCACTCGCTTAAAGTTT+TGG | 0.323243 | 4.3:-71894721 | MS.gene48875:CDS |
| TAGATTACCAACTACTTTGA+AGG | 0.357772 | 4.3:-71895087 | MS.gene48875:CDS |
| TCTCTTTGGAATACTTTCTT+CGG | 0.374925 | 4.3:-71894826 | MS.gene48875:CDS |
| CTTCCAAATCCAATACAATA+TGG | 0.375006 | 4.3:+71895735 | None:intergenic |
| CAACTAGGTCAGCTGGATTT+GGG | 0.375194 | 4.3:+71894601 | None:intergenic |
| TGCATCTACTGCTCGCTAAT+CGG | 0.377217 | 4.3:-71894912 | MS.gene48875:CDS |
| TTCTAATTCTGCATACTGAT+AGG | 0.378805 | 4.3:+71895687 | None:intergenic |
| TTTGGGTTGTGCTCTTCGTA+TGG | 0.396092 | 4.3:-71894767 | MS.gene48875:CDS |
| TTGGGTTGTGCTCTTCGTAT+GGG | 0.402906 | 4.3:-71894766 | MS.gene48875:CDS |
| AAAGTATTCCAAAGAGAAGA+AGG | 0.428632 | 4.3:+71894832 | None:intergenic |
| GGTGTTGATATTCAATACTA+AGG | 0.429220 | 4.3:-71894655 | MS.gene48875:CDS |
| TCTTGATCCTTCAAAGTAGT+TGG | 0.429294 | 4.3:+71895080 | None:intergenic |
| GAGTGACGAGCAAGTATAAC+TGG | 0.443034 | 4.3:+71894735 | None:intergenic |
| ATGGCATGGCATGGGATTGA+AGG | 0.461512 | 4.3:+71895050 | None:intergenic |
| CTACCTTCAACTAGGTCAGC+TGG | 0.475286 | 4.3:+71894594 | None:intergenic |
| AATCCAGCTGACCTAGTTGA+AGG | 0.483463 | 4.3:-71894597 | MS.gene48875:CDS |
| TTTCTTGACACAATACTCAT+TGG | 0.492166 | 4.3:-71895020 | MS.gene48875:CDS |
| GGAAGAACATGAAGATGTAA+AGG | 0.495227 | 4.3:-71895717 | MS.gene48875:CDS |
| ATGAACTTTGTGATCATCAA+AGG | 0.497786 | 4.3:+71894959 | None:intergenic |
| AACACAAAGATGGCATGGCA+TGG | 0.520422 | 4.3:+71895041 | None:intergenic |
| GTGTAATGACCATATTGTAT+TGG | 0.548354 | 4.3:-71895744 | MS.gene48875:CDS |
| ACACAAAGATGGCATGGCAT+GGG | 0.550895 | 4.3:+71895042 | None:intergenic |
| CGGCAGCAAGATGGAGAGAA+GGG | 0.553496 | 4.3:+71894800 | None:intergenic |
| TGGGTTGTGCTCTTCGTATG+GGG | 0.556531 | 4.3:-71894765 | MS.gene48875:CDS |
| AAGTTGTCGCGGCAGCAAGA+TGG | 0.558737 | 4.3:+71894791 | None:intergenic |
| TGAACTTTGTGATCATCAAA+GGG | 0.562970 | 4.3:+71894960 | None:intergenic |
| GATGCAGATGGATGTCAAGA+AGG | 0.564412 | 4.3:+71894929 | None:intergenic |
| ATTAAGATCTACCTTCAACT+AGG | 0.573127 | 4.3:+71894586 | None:intergenic |
| TGGCATGGCATGGGATTGAA+GGG | 0.575309 | 4.3:+71895051 | None:intergenic |
| TAGGTCAGCTGGATTTGGGT+AGG | 0.578541 | 4.3:+71894605 | None:intergenic |
| TGTGTCAAGAAACACAAAGA+TGG | 0.584873 | 4.3:+71895031 | None:intergenic |
| CTTGATTATAGTATTTCACA+CGG | 0.587536 | 4.3:+71895600 | None:intergenic |
| CAAGAAACACAAAGATGGCA+TGG | 0.592259 | 4.3:+71895036 | None:intergenic |
| GCGGCAGCAAGATGGAGAGA+AGG | 0.593311 | 4.3:+71894799 | None:intergenic |
| AAGTATTCCAAAGAGAAGAA+GGG | 0.599617 | 4.3:+71894833 | None:intergenic |
| GTGTCGGATAATAGAAGCGA+TGG | 0.602232 | 4.3:-71894697 | MS.gene48875:CDS |
| TAGCGAGCAGTAGATGCAGA+TGG | 0.605364 | 4.3:+71894917 | None:intergenic |
| TGAAATACTATAATCAAGCA+AGG | 0.609382 | 4.3:-71895595 | MS.gene48875:intron |
| GGTAGTAAAGATTACTACCA+TGG | 0.620964 | 4.3:-71894676 | MS.gene48875:CDS |
| AATACTAAGGATTCAGCCAG+TGG | 0.626440 | 4.3:-71894642 | MS.gene48875:CDS |
| AAGATCTACACGAAAACTCG+TGG | 0.650237 | 4.3:-71894885 | MS.gene48875:CDS |
| TTGATTATAGTATTTCACAC+GGG | 0.658993 | 4.3:+71895601 | None:intergenic |
| AGTATTGAATATCAACACCA+TGG | 0.687774 | 4.3:+71894659 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATTAATTTAATAGTAC+TGG | - | chr4.3:71895027-71895046 | MS.gene48875:CDS | 10.0% |
| !! | CAATTAAAATAAATAAAAGA+GGG | + | chr4.3:71895096-71895115 | None:intergenic | 10.0% |
| !!! | ATATGTTTTATATATCTAAT+CGG | - | chr4.3:71895223-71895242 | MS.gene48875:intron | 10.0% |
| !!! | CTCTTTTATTTATTTTAATT+GGG | - | chr4.3:71895095-71895114 | MS.gene48875:intron | 10.0% |
| !! | ACATTGTCAAAAAAAAAATT+AGG | + | chr4.3:71895190-71895209 | None:intergenic | 15.0% |
| !! | CATTGTCAAAAAAAAAATTA+GGG | + | chr4.3:71895189-71895208 | None:intergenic | 15.0% |
| !! | CCAATTAAAATAAATAAAAG+AGG | + | chr4.3:71895097-71895116 | None:intergenic | 15.0% |
| !!! | CCTCTTTTATTTATTTTAAT+TGG | - | chr4.3:71895094-71895113 | MS.gene48875:intron | 15.0% |
| !!! | TAGCCTTATTTAAATATTTT+GGG | + | chr4.3:71894985-71895004 | None:intergenic | 15.0% |
| !!! | TATTATTTTGTTTTTTTGAC+TGG | - | chr4.3:71894811-71894830 | MS.gene48875:CDS | 15.0% |
| !!! | TATTTTTGTTTATTATGGTT+TGG | - | chr4.3:71895145-71895164 | MS.gene48875:intron | 15.0% |
| !!! | TTTTGACAATGTTTTATATA+TGG | - | chr4.3:71895198-71895217 | MS.gene48875:intron | 15.0% |
| !!! | TTTTTATGATACTTTGTTTA+CGG | + | chr4.3:71894935-71894954 | None:intergenic | 15.0% |
| !! | AGTGAAGAATCATATAAAAT+AGG | + | chr4.3:71894959-71894978 | None:intergenic | 20.0% |
| !! | GAACCCAAAATATTTAAATA+AGG | - | chr4.3:71894979-71894998 | MS.gene48875:CDS | 20.0% |
| !! | GTGAAGAATCATATAAAATA+GGG | + | chr4.3:71894958-71894977 | None:intergenic | 20.0% |
| !! | TAATTACTCTTTCCATTATA+TGG | - | chr4.3:71894770-71894789 | MS.gene48875:CDS | 20.0% |
| !!! | AGAACTTTTAAACCATATAA+TGG | + | chr4.3:71894785-71894804 | None:intergenic | 20.0% |
| !!! | CTAGCCTTATTTAAATATTT+TGG | + | chr4.3:71894986-71895005 | None:intergenic | 20.0% |
| !!! | GCACATATTTTTGTTTATTA+TGG | - | chr4.3:71895140-71895159 | MS.gene48875:intron | 20.0% |
| ! | AAAAGAGGGTAGTTAATAAA+AGG | + | chr4.3:71895082-71895101 | None:intergenic | 25.0% |
| ! | CTTGATTATAGTATTTCACA+CGG | + | chr4.3:71894742-71894761 | None:intergenic | 25.0% |
| ! | TACGGATTTGATTAAACATA+AGG | + | chr4.3:71894917-71894936 | None:intergenic | 25.0% |
| ! | TAGAAAAAGAAGACTTAAAG+TGG | - | chr4.3:71894670-71894689 | MS.gene48875:CDS | 25.0% |
| ! | TGAAATACTATAATCAAGCA+AGG | - | chr4.3:71894744-71894763 | MS.gene48875:CDS | 25.0% |
| ! | TTGATTATAGTATTTCACAC+GGG | + | chr4.3:71894741-71894760 | None:intergenic | 25.0% |
| !! | TCACTAACTTCAATTTTCTT+TGG | + | chr4.3:71894709-71894728 | None:intergenic | 25.0% |
| !!! | CAAAGCTAAGAATTTTTTCT+TGG | + | chr4.3:71895486-71895505 | None:intergenic | 25.0% |
| AAGTATTCCAAAGAGAAGAA+GGG | + | chr4.3:71895509-71895528 | None:intergenic | 30.0% | |
| ATGAACTTTGTGATCATCAA+AGG | + | chr4.3:71895383-71895402 | None:intergenic | 30.0% | |
| CTTCCAAATCCAATACAATA+TGG | + | chr4.3:71894607-71894626 | None:intergenic | 30.0% | |
| GTGTAATGACCATATTGTAT+TGG | - | chr4.3:71894595-71894614 | MS.gene48875:CDS | 30.0% | |
| TAGATTACCAACTACTTTGA+AGG | - | chr4.3:71895252-71895271 | MS.gene48875:intron | 30.0% | |
| TGAACTTTGTGATCATCAAA+GGG | + | chr4.3:71895382-71895401 | None:intergenic | 30.0% | |
| TGACCATATTGTATTGGATT+TGG | - | chr4.3:71894601-71894620 | MS.gene48875:CDS | 30.0% | |
| TTCTAATTCTGCATACTGAT+AGG | + | chr4.3:71894655-71894674 | None:intergenic | 30.0% | |
| TTTCTTGACACAATACTCAT+TGG | - | chr4.3:71895319-71895338 | MS.gene48875:intron | 30.0% | |
| ! | AAAGTATTCCAAAGAGAAGA+AGG | + | chr4.3:71895510-71895529 | None:intergenic | 30.0% |
| ! | AGTATTGAATATCAACACCA+TGG | + | chr4.3:71895683-71895702 | None:intergenic | 30.0% |
| !! | GGTGTTGATATTCAATACTA+AGG | - | chr4.3:71895684-71895703 | MS.gene48875:CDS | 30.0% |
| !! | TCTCTTTGGAATACTTTCTT+CGG | - | chr4.3:71895513-71895532 | MS.gene48875:intron | 30.0% |
| !! | TTTTCTTTGGTTTACTCTCA+TGG | + | chr4.3:71894696-71894715 | None:intergenic | 30.0% |
| !!! | AGCTAAGAATTTTTTCTTGG+TGG | + | chr4.3:71895483-71895502 | None:intergenic | 30.0% |
| GGAAGAACATGAAGATGTAA+AGG | - | chr4.3:71894622-71894641 | MS.gene48875:CDS | 35.0% | |
| GGGTCAAATTTCTAACAACT+AGG | - | chr4.3:71895115-71895134 | MS.gene48875:intron | 35.0% | |
| GGTAGTAAAGATTACTACCA+TGG | - | chr4.3:71895663-71895682 | MS.gene48875:CDS | 35.0% | |
| TCTTGATCCTTCAAAGTAGT+TGG | + | chr4.3:71895262-71895281 | None:intergenic | 35.0% | |
| TGTGTCAAGAAACACAAAGA+TGG | + | chr4.3:71895311-71895330 | None:intergenic | 35.0% | |
| AAGATCTACACGAAAACTCG+TGG | - | chr4.3:71895454-71895473 | MS.gene48875:intron | 40.0% | |
| AATACTAAGGATTCAGCCAG+TGG | - | chr4.3:71895697-71895716 | MS.gene48875:CDS | 40.0% | |
| ACAACCCAAAAAAGTTGTCG+CGG | + | chr4.3:71895562-71895581 | None:intergenic | 40.0% | |
| CAAGAAACACAAAGATGGCA+TGG | + | chr4.3:71895306-71895325 | None:intergenic | 40.0% | |
| ! | TCGTCACTCGCTTAAAGTTT+TGG | - | chr4.3:71895618-71895637 | MS.gene48875:CDS | 40.0% |
| !! | CGCTTAAAGTTTTGGTGTGT+CGG | - | chr4.3:71895626-71895645 | MS.gene48875:CDS | 40.0% |
| AACACAAAGATGGCATGGCA+TGG | + | chr4.3:71895301-71895320 | None:intergenic | 45.0% | |
| AATCCAGCTGACCTAGTTGA+AGG | - | chr4.3:71895742-71895761 | MS.gene48875:CDS | 45.0% | |
| ACACAAAGATGGCATGGCAT+GGG | + | chr4.3:71895300-71895319 | None:intergenic | 45.0% | |
| AGCTTTGCCCTTCTTCTCTT+TGG | - | chr4.3:71895499-71895518 | MS.gene48875:intron | 45.0% | |
| CAACTAGGTCAGCTGGATTT+GGG | + | chr4.3:71895741-71895760 | None:intergenic | 45.0% | |
| GAGTGACGAGCAAGTATAAC+TGG | + | chr4.3:71895607-71895626 | None:intergenic | 45.0% | |
| GATGCAGATGGATGTCAAGA+AGG | + | chr4.3:71895413-71895432 | None:intergenic | 45.0% | |
| GTGTCGGATAATAGAAGCGA+TGG | - | chr4.3:71895642-71895661 | MS.gene48875:CDS | 45.0% | |
| TCAACTAGGTCAGCTGGATT+TGG | + | chr4.3:71895742-71895761 | None:intergenic | 45.0% | |
| TGCATCTACTGCTCGCTAAT+CGG | - | chr4.3:71895427-71895446 | MS.gene48875:intron | 45.0% | |
| ! | TTGGGTTGTGCTCTTCGTAT+GGG | - | chr4.3:71895573-71895592 | MS.gene48875:intron | 45.0% |
| !! | GGTTGTTTTGTTGCTTCCAC+TGG | + | chr4.3:71895716-71895735 | None:intergenic | 45.0% |
| !! | TTTGGGTTGTGCTCTTCGTA+TGG | - | chr4.3:71895572-71895591 | MS.gene48875:intron | 45.0% |
| ATGGCATGGCATGGGATTGA+AGG | + | chr4.3:71895292-71895311 | None:intergenic | 50.0% | |
| CTACCTTCAACTAGGTCAGC+TGG | + | chr4.3:71895748-71895767 | None:intergenic | 50.0% | |
| TAGCGAGCAGTAGATGCAGA+TGG | + | chr4.3:71895425-71895444 | None:intergenic | 50.0% | |
| TAGGTCAGCTGGATTTGGGT+AGG | + | chr4.3:71895737-71895756 | None:intergenic | 50.0% | |
| TGGCATGGCATGGGATTGAA+GGG | + | chr4.3:71895291-71895310 | None:intergenic | 50.0% | |
| TGGGTTGTGCTCTTCGTATG+GGG | - | chr4.3:71895574-71895593 | MS.gene48875:intron | 50.0% | |
| !!! | GCTGCCGCGACAACTTTTTT+GGG | - | chr4.3:71895555-71895574 | MS.gene48875:intron | 50.0% |
| !!! | TGCTGCCGCGACAACTTTTT+TGG | - | chr4.3:71895554-71895573 | MS.gene48875:intron | 50.0% |
| AAGTTGTCGCGGCAGCAAGA+TGG | + | chr4.3:71895551-71895570 | None:intergenic | 55.0% | |
| CGGCAGCAAGATGGAGAGAA+GGG | + | chr4.3:71895542-71895561 | None:intergenic | 55.0% | |
| GCGGCAGCAAGATGGAGAGA+AGG | + | chr4.3:71895543-71895562 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 71894587 | 71895774 | 71894587 | ID=MS.gene48875 |
| chr4.3 | mRNA | 71894587 | 71895774 | 71894587 | ID=MS.gene48875.t1;Parent=MS.gene48875 |
| chr4.3 | exon | 71895596 | 71895774 | 71895596 | ID=MS.gene48875.t1.exon1;Parent=MS.gene48875.t1 |
| chr4.3 | CDS | 71895596 | 71895774 | 71895596 | ID=cds.MS.gene48875.t1;Parent=MS.gene48875.t1 |
| chr4.3 | exon | 71894587 | 71895106 | 71894587 | ID=MS.gene48875.t1.exon2;Parent=MS.gene48875.t1 |
| chr4.3 | CDS | 71894587 | 71895106 | 71894587 | ID=cds.MS.gene48875.t1;Parent=MS.gene48875.t1 |
| Gene Sequence |
| Protein sequence |
