Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059265.t1 | KEH29169.1 | 94.8 | 232 | 12 | 0 | 1 | 232 | 1 | 232 | 1.30E-116 | 429.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059265.t1 | A0A072UHD2 | 94.8 | 232 | 12 | 0 | 1 | 232 | 1 | 232 | 9.4e-117 | 429.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene059265 | 0.838956 | 2.11E-57 | -1.69E-46 |
| MS.gene059265 | MS.gene059955 | 0.816841 | 4.49E-52 | -1.69E-46 |
| MS.gene059265 | MS.gene062678 | 0.825263 | 5.16E-54 | -1.69E-46 |
| MS.gene059265 | MS.gene062682 | 0.845918 | 3.01E-59 | -1.69E-46 |
| MS.gene059265 | MS.gene062683 | 0.839463 | 1.56E-57 | -1.69E-46 |
| MS.gene059265 | MS.gene062686 | 0.843954 | 1.02E-58 | -1.69E-46 |
| MS.gene059265 | MS.gene062687 | 0.831661 | 1.47E-55 | -1.69E-46 |
| MS.gene059265 | MS.gene062690 | 0.811883 | 5.60E-51 | -1.69E-46 |
| MS.gene059265 | MS.gene066500 | 0.813072 | 3.08E-51 | -1.69E-46 |
| MS.gene059265 | MS.gene066602 | 0.80602 | 1.01E-49 | -1.69E-46 |
| MS.gene059265 | MS.gene24862 | 0.804367 | 2.23E-49 | -1.69E-46 |
| MS.gene059265 | MS.gene28548 | 0.803754 | 3.00E-49 | -1.69E-46 |
| MS.gene059265 | MS.gene33905 | 0.804527 | 2.07E-49 | -1.69E-46 |
| MS.gene059265 | MS.gene37747 | 0.801027 | 1.09E-48 | -1.69E-46 |
| MS.gene059265 | MS.gene47721 | 0.828699 | 7.77E-55 | -1.69E-46 |
| MS.gene059265 | MS.gene48021 | 0.810868 | 9.31E-51 | -1.69E-46 |
| MS.gene059265 | MS.gene48805 | 0.802616 | 5.15E-49 | -1.69E-46 |
| MS.gene059265 | MS.gene48875 | 0.871969 | 4.56E-67 | -1.69E-46 |
| MS.gene059265 | MS.gene52370 | 0.801706 | 7.93E-49 | -1.69E-46 |
| MS.gene059265 | MS.gene52373 | 0.805061 | 1.60E-49 | -1.69E-46 |
| MS.gene059265 | MS.gene60531 | 0.822301 | 2.55E-53 | -1.69E-46 |
| MS.gene059265 | MS.gene63444 | 0.800407 | 1.46E-48 | -1.69E-46 |
| MS.gene059265 | MS.gene69966 | 0.814672 | 1.37E-51 | -1.69E-46 |
| MS.gene059265 | MS.gene74774 | 0.815932 | 7.18E-52 | -1.69E-46 |
| MS.gene059265 | MS.gene76677 | 0.900584 | 6.33E-78 | -1.69E-46 |
| MS.gene059265 | MS.gene76738 | 0.807781 | 4.27E-50 | -1.69E-46 |
| MS.gene059265 | MS.gene81504 | 0.805107 | 1.57E-49 | -1.69E-46 |
| MS.gene059265 | MS.gene82183 | 0.940509 | 2.07E-100 | -1.69E-46 |
| MS.gene059265 | MS.gene87056 | 0.806236 | 9.07E-50 | -1.69E-46 |
| MS.gene059265 | MS.gene87750 | 0.803838 | 2.88E-49 | -1.69E-46 |
| MS.gene059265 | MS.gene91223 | 0.816765 | 4.67E-52 | -1.69E-46 |
| MS.gene059265 | MS.gene95804 | 0.802716 | 4.92E-49 | -1.69E-46 |
| MS.gene059265 | MS.gene98322 | 0.801201 | 1.01E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059265.t1 | MTR_4g027195 | 94.828 | 232 | 12 | 0 | 1 | 232 | 1 | 232 | 1.81e-154 | 428 |
| MS.gene059265.t1 | MTR_4g027105 | 67.836 | 171 | 49 | 3 | 60 | 228 | 25 | 191 | 2.27e-70 | 214 |
| MS.gene059265.t1 | MTR_4g027225 | 66.082 | 171 | 52 | 3 | 60 | 228 | 25 | 191 | 1.59e-58 | 183 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTTGCCCTTCTTCTCTT+TGG | 0.204053 | 4.4:-75045383 | MS.gene059265:CDS |
| TCAACTAGGTCAGCTGGATT+TGG | 0.237066 | 4.4:+75045143 | None:intergenic |
| CTAACATCCTTTACATCTTC+AGG | 0.271060 | 4.4:+75046253 | None:intergenic |
| TCGTCACTCGCTTAAAGTTT+TGG | 0.323243 | 4.4:-75045264 | MS.gene059265:CDS |
| CTTCCAAATCCAACACAATA+TGG | 0.331832 | 4.4:+75046278 | None:intergenic |
| TGACCATATTGTGTTGGATT+TGG | 0.351532 | 4.4:-75046281 | MS.gene059265:CDS |
| TAGATTACCAACTACTTTGA+AGG | 0.357772 | 4.4:-75045630 | MS.gene059265:CDS |
| TCTCTTTGGAATACTTTCTT+CGG | 0.374925 | 4.4:-75045369 | MS.gene059265:CDS |
| CAACTAGGTCAGCTGGATTT+GGG | 0.375194 | 4.4:+75045144 | None:intergenic |
| TGCATCTACTGCTCGCTAAT+CGG | 0.377217 | 4.4:-75045455 | MS.gene059265:CDS |
| TTCTAATTCTGCATACTGAT+AGG | 0.378805 | 4.4:+75046230 | None:intergenic |
| TTTGGGTTGTGCTCTTCGTA+TGG | 0.396092 | 4.4:-75045310 | MS.gene059265:CDS |
| TTGGGTTGTGCTCTTCGTAT+GGG | 0.402906 | 4.4:-75045309 | MS.gene059265:CDS |
| AAAGTATTCCAAAGAGAAGA+AGG | 0.428632 | 4.4:+75045375 | None:intergenic |
| TCTTGATCCTTCAAAGTAGT+TGG | 0.429294 | 4.4:+75045623 | None:intergenic |
| GGTGTTGATATTCAATACTA+AGG | 0.435310 | 4.4:-75045198 | MS.gene059265:CDS |
| GAGTGACGAGCAAGTATAAC+TGG | 0.443034 | 4.4:+75045278 | None:intergenic |
| ATGGCATGGCATGGGATTGA+AGG | 0.461512 | 4.4:+75045593 | None:intergenic |
| CTACCTTCAACTAGGTCAGC+TGG | 0.475286 | 4.4:+75045137 | None:intergenic |
| AATCCAGCTGACCTAGTTGA+AGG | 0.483463 | 4.4:-75045140 | MS.gene059265:CDS |
| TTTCTTGACACAATACTCAT+TGG | 0.492166 | 4.4:-75045563 | MS.gene059265:CDS |
| ATGAACTTTGTGATCATCAA+AGG | 0.497786 | 4.4:+75045502 | None:intergenic |
| GGAAGAACCTGAAGATGTAA+AGG | 0.518106 | 4.4:-75046260 | MS.gene059265:CDS |
| AACACAAAGATGGCATGGCA+TGG | 0.520422 | 4.4:+75045584 | None:intergenic |
| ACACAAAGATGGCATGGCAT+GGG | 0.550895 | 4.4:+75045585 | None:intergenic |
| CGGCAGCAAGATGGAGAGAA+GGG | 0.553496 | 4.4:+75045343 | None:intergenic |
| TGGGTTGTGCTCTTCGTATG+GGG | 0.556531 | 4.4:-75045308 | MS.gene059265:CDS |
| AAGTTGTCGCGGCAGCAAGA+TGG | 0.558737 | 4.4:+75045334 | None:intergenic |
| TGAACTTTGTGATCATCAAA+GGG | 0.562970 | 4.4:+75045503 | None:intergenic |
| GATGCAGATGGATGTCAAGA+AGG | 0.564412 | 4.4:+75045472 | None:intergenic |
| ATTAAGATCTACCTTCAACT+AGG | 0.573127 | 4.4:+75045129 | None:intergenic |
| TGGCATGGCATGGGATTGAA+GGG | 0.575309 | 4.4:+75045594 | None:intergenic |
| TAGGTCAGCTGGATTTGGGT+AGG | 0.578541 | 4.4:+75045148 | None:intergenic |
| TGTGTCAAGAAACACAAAGA+TGG | 0.584873 | 4.4:+75045574 | None:intergenic |
| CTTGATTATAGTATTTCACA+CGG | 0.587536 | 4.4:+75046143 | None:intergenic |
| AGTATTGAATATCAACACCT+TGG | 0.588376 | 4.4:+75045202 | None:intergenic |
| CAAGAAACACAAAGATGGCA+TGG | 0.592259 | 4.4:+75045579 | None:intergenic |
| GCGGCAGCAAGATGGAGAGA+AGG | 0.593311 | 4.4:+75045342 | None:intergenic |
| AAGTATTCCAAAGAGAAGAA+GGG | 0.599617 | 4.4:+75045376 | None:intergenic |
| GTGTCGGATAATAGAAGCGA+TGG | 0.602232 | 4.4:-75045240 | MS.gene059265:CDS |
| TAGCGAGCAGTAGATGCAGA+TGG | 0.605364 | 4.4:+75045460 | None:intergenic |
| TGAAATACTATAATCAAGCA+AGG | 0.609382 | 4.4:-75046138 | MS.gene059265:intron |
| AATACTAAGGATTCAGCCAG+TGG | 0.626440 | 4.4:-75045185 | MS.gene059265:CDS |
| GGTAGTAAAGATTACTACCA+AGG | 0.634715 | 4.4:-75045219 | MS.gene059265:CDS |
| AAGATCTACACGAAGACTCG+TGG | 0.646014 | 4.4:-75045428 | MS.gene059265:CDS |
| TTGATTATAGTATTTCACAC+GGG | 0.658993 | 4.4:+75046144 | None:intergenic |
| GCGTAATGACCATATTGTGT+TGG | 0.694679 | 4.4:-75046287 | MS.gene059265:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATTAATTTAATAGTAC+TGG | - | chr4.4:75045570-75045589 | MS.gene059265:CDS | 10.0% |
| !! | CAATTAAAATAAATAAAAGA+GGG | + | chr4.4:75045639-75045658 | None:intergenic | 10.0% |
| !!! | ATATGTTTTATATATCTAAT+CGG | - | chr4.4:75045766-75045785 | MS.gene059265:intron | 10.0% |
| !!! | CTCTTTTATTTATTTTAATT+GGG | - | chr4.4:75045638-75045657 | MS.gene059265:intron | 10.0% |
| !! | CCAATTAAAATAAATAAAAG+AGG | + | chr4.4:75045640-75045659 | None:intergenic | 15.0% |
| !!! | CCTCTTTTATTTATTTTAAT+TGG | - | chr4.4:75045637-75045656 | MS.gene059265:intron | 15.0% |
| !!! | TAGCCTTATTTAAATATTTT+GGG | + | chr4.4:75045528-75045547 | None:intergenic | 15.0% |
| !!! | TATTATTTTGTTTTTTTGAC+TGG | - | chr4.4:75045354-75045373 | MS.gene059265:CDS | 15.0% |
| !!! | TATTTTTGTTTATTATGGTT+TGG | - | chr4.4:75045688-75045707 | MS.gene059265:intron | 15.0% |
| !!! | TTTTTATGATACTTTGTTTA+CGG | + | chr4.4:75045478-75045497 | None:intergenic | 15.0% |
| !! | ACATTGTCAAAAAAAAACTT+AGG | + | chr4.4:75045733-75045752 | None:intergenic | 20.0% |
| !! | AGTGAAGAATCATATAAAAT+AGG | + | chr4.4:75045502-75045521 | None:intergenic | 20.0% |
| !! | CATTGTCAAAAAAAAACTTA+GGG | + | chr4.4:75045732-75045751 | None:intergenic | 20.0% |
| !! | GAACCCAAAATATTTAAATA+AGG | - | chr4.4:75045522-75045541 | MS.gene059265:CDS | 20.0% |
| !! | GTGAAGAATCATATAAAATA+GGG | + | chr4.4:75045501-75045520 | None:intergenic | 20.0% |
| !! | TAATTACTCTTTCCATTATA+TGG | - | chr4.4:75045313-75045332 | MS.gene059265:CDS | 20.0% |
| !!! | AGAACTTTTAAACCATATAA+TGG | + | chr4.4:75045328-75045347 | None:intergenic | 20.0% |
| !!! | CTAGCCTTATTTAAATATTT+TGG | + | chr4.4:75045529-75045548 | None:intergenic | 20.0% |
| !!! | GCACATATTTTTGTTTATTA+TGG | - | chr4.4:75045683-75045702 | MS.gene059265:intron | 20.0% |
| !!! | TTTTGACAATGTTTTATGTA+TGG | - | chr4.4:75045741-75045760 | MS.gene059265:intron | 20.0% |
| ! | AAAAGAGGGTAGTTAATAAA+AGG | + | chr4.4:75045625-75045644 | None:intergenic | 25.0% |
| ! | CTTGATTATAGTATTTCACA+CGG | + | chr4.4:75045285-75045304 | None:intergenic | 25.0% |
| ! | TACGGATTTGATTAAACATA+AGG | + | chr4.4:75045460-75045479 | None:intergenic | 25.0% |
| ! | TAGAAAAAGAAGACTTATAG+TGG | - | chr4.4:75045213-75045232 | MS.gene059265:CDS | 25.0% |
| ! | TGAAATACTATAATCAAGCA+AGG | - | chr4.4:75045287-75045306 | MS.gene059265:CDS | 25.0% |
| ! | TTGATTATAGTATTTCACAC+GGG | + | chr4.4:75045284-75045303 | None:intergenic | 25.0% |
| !! | TCACTAACTTCAATTTTCTT+TGG | + | chr4.4:75045252-75045271 | None:intergenic | 25.0% |
| !!! | CAAAGCTAAGAATTTTTTCT+TGG | + | chr4.4:75046029-75046048 | None:intergenic | 25.0% |
| AAGTATTCCAAAGAGAAGAA+GGG | + | chr4.4:75046052-75046071 | None:intergenic | 30.0% | |
| ATGAACTTTGTGATCATCAA+AGG | + | chr4.4:75045926-75045945 | None:intergenic | 30.0% | |
| TAGATTACCAACTACTTTGA+AGG | - | chr4.4:75045795-75045814 | MS.gene059265:intron | 30.0% | |
| TGAACTTTGTGATCATCAAA+GGG | + | chr4.4:75045925-75045944 | None:intergenic | 30.0% | |
| TTCTAATTCTGCATACTGAT+AGG | + | chr4.4:75045198-75045217 | None:intergenic | 30.0% | |
| TTTCTTGACACAATACTCAT+TGG | - | chr4.4:75045862-75045881 | MS.gene059265:intron | 30.0% | |
| ! | AAAGTATTCCAAAGAGAAGA+AGG | + | chr4.4:75046053-75046072 | None:intergenic | 30.0% |
| ! | AGTATTGAATATCAACACCT+TGG | + | chr4.4:75046226-75046245 | None:intergenic | 30.0% |
| !! | GGTGTTGATATTCAATACTA+AGG | - | chr4.4:75046227-75046246 | MS.gene059265:CDS | 30.0% |
| !! | TCTCTTTGGAATACTTTCTT+CGG | - | chr4.4:75046056-75046075 | MS.gene059265:intron | 30.0% |
| !! | TTTTCTTTGGTTTACTCTCA+TGG | + | chr4.4:75045239-75045258 | None:intergenic | 30.0% |
| !!! | AGCTAAGAATTTTTTCTTGG+TGG | + | chr4.4:75046026-75046045 | None:intergenic | 30.0% |
| CTAACATCCTTTACATCTTC+AGG | + | chr4.4:75045175-75045194 | None:intergenic | 35.0% | |
| CTTCCAAATCCAACACAATA+TGG | + | chr4.4:75045150-75045169 | None:intergenic | 35.0% | |
| GGGTCAAATTTCTAACAACT+AGG | - | chr4.4:75045658-75045677 | MS.gene059265:intron | 35.0% | |
| GGTAGTAAAGATTACTACCA+AGG | - | chr4.4:75046206-75046225 | MS.gene059265:CDS | 35.0% | |
| TCTTGATCCTTCAAAGTAGT+TGG | + | chr4.4:75045805-75045824 | None:intergenic | 35.0% | |
| TGACCATATTGTGTTGGATT+TGG | - | chr4.4:75045144-75045163 | MS.gene059265:CDS | 35.0% | |
| TGTGTCAAGAAACACAAAGA+TGG | + | chr4.4:75045854-75045873 | None:intergenic | 35.0% | |
| AATACTAAGGATTCAGCCAG+TGG | - | chr4.4:75046240-75046259 | MS.gene059265:CDS | 40.0% | |
| ACAACCCAAAAAAGTTGTCG+CGG | + | chr4.4:75046105-75046124 | None:intergenic | 40.0% | |
| CAAGAAACACAAAGATGGCA+TGG | + | chr4.4:75045849-75045868 | None:intergenic | 40.0% | |
| GCGTAATGACCATATTGTGT+TGG | - | chr4.4:75045138-75045157 | MS.gene059265:CDS | 40.0% | |
| GGAAGAACCTGAAGATGTAA+AGG | - | chr4.4:75045165-75045184 | MS.gene059265:CDS | 40.0% | |
| ! | TCGTCACTCGCTTAAAGTTT+TGG | - | chr4.4:75046161-75046180 | MS.gene059265:CDS | 40.0% |
| !! | CGCTTAAAGTTTTGGTGTGT+CGG | - | chr4.4:75046169-75046188 | MS.gene059265:CDS | 40.0% |
| AACACAAAGATGGCATGGCA+TGG | + | chr4.4:75045844-75045863 | None:intergenic | 45.0% | |
| AAGATCTACACGAAGACTCG+TGG | - | chr4.4:75045997-75046016 | MS.gene059265:intron | 45.0% | |
| AATCCAGCTGACCTAGTTGA+AGG | - | chr4.4:75046285-75046304 | MS.gene059265:CDS | 45.0% | |
| ACACAAAGATGGCATGGCAT+GGG | + | chr4.4:75045843-75045862 | None:intergenic | 45.0% | |
| AGCTTTGCCCTTCTTCTCTT+TGG | - | chr4.4:75046042-75046061 | MS.gene059265:intron | 45.0% | |
| CAACTAGGTCAGCTGGATTT+GGG | + | chr4.4:75046284-75046303 | None:intergenic | 45.0% | |
| GAGTGACGAGCAAGTATAAC+TGG | + | chr4.4:75046150-75046169 | None:intergenic | 45.0% | |
| GATGCAGATGGATGTCAAGA+AGG | + | chr4.4:75045956-75045975 | None:intergenic | 45.0% | |
| GTGTCGGATAATAGAAGCGA+TGG | - | chr4.4:75046185-75046204 | MS.gene059265:CDS | 45.0% | |
| TCAACTAGGTCAGCTGGATT+TGG | + | chr4.4:75046285-75046304 | None:intergenic | 45.0% | |
| TGCATCTACTGCTCGCTAAT+CGG | - | chr4.4:75045970-75045989 | MS.gene059265:intron | 45.0% | |
| ! | TTGGGTTGTGCTCTTCGTAT+GGG | - | chr4.4:75046116-75046135 | MS.gene059265:intron | 45.0% |
| !! | GGTTGTTTTGTTGCTTCCAC+TGG | + | chr4.4:75046259-75046278 | None:intergenic | 45.0% |
| !! | TTTGGGTTGTGCTCTTCGTA+TGG | - | chr4.4:75046115-75046134 | MS.gene059265:intron | 45.0% |
| ATGGCATGGCATGGGATTGA+AGG | + | chr4.4:75045835-75045854 | None:intergenic | 50.0% | |
| CTACCTTCAACTAGGTCAGC+TGG | + | chr4.4:75046291-75046310 | None:intergenic | 50.0% | |
| TAGCGAGCAGTAGATGCAGA+TGG | + | chr4.4:75045968-75045987 | None:intergenic | 50.0% | |
| TAGGTCAGCTGGATTTGGGT+AGG | + | chr4.4:75046280-75046299 | None:intergenic | 50.0% | |
| TGGCATGGCATGGGATTGAA+GGG | + | chr4.4:75045834-75045853 | None:intergenic | 50.0% | |
| TGGGTTGTGCTCTTCGTATG+GGG | - | chr4.4:75046117-75046136 | MS.gene059265:intron | 50.0% | |
| !!! | GCTGCCGCGACAACTTTTTT+GGG | - | chr4.4:75046098-75046117 | MS.gene059265:intron | 50.0% |
| !!! | TGCTGCCGCGACAACTTTTT+TGG | - | chr4.4:75046097-75046116 | MS.gene059265:intron | 50.0% |
| AAGTTGTCGCGGCAGCAAGA+TGG | + | chr4.4:75046094-75046113 | None:intergenic | 55.0% | |
| CGGCAGCAAGATGGAGAGAA+GGG | + | chr4.4:75046085-75046104 | None:intergenic | 55.0% | |
| GCGGCAGCAAGATGGAGAGA+AGG | + | chr4.4:75046086-75046105 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 75045130 | 75046317 | 75045130 | ID=MS.gene059265 |
| chr4.4 | mRNA | 75045130 | 75046317 | 75045130 | ID=MS.gene059265.t1;Parent=MS.gene059265 |
| chr4.4 | exon | 75046139 | 75046317 | 75046139 | ID=MS.gene059265.t1.exon1;Parent=MS.gene059265.t1 |
| chr4.4 | CDS | 75046139 | 75046317 | 75046139 | ID=cds.MS.gene059265.t1;Parent=MS.gene059265.t1 |
| chr4.4 | exon | 75045130 | 75045649 | 75045130 | ID=MS.gene059265.t1.exon2;Parent=MS.gene059265.t1 |
| chr4.4 | CDS | 75045130 | 75045649 | 75045130 | ID=cds.MS.gene059265.t1;Parent=MS.gene059265.t1 |
| Gene Sequence |
| Protein sequence |
