Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48986.t1 | XP_003605133.2 | 100 | 119 | 0 | 0 | 4 | 122 | 33 | 151 | 1.10E-58 | 235.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48986.t1 | P46298 | 97.5 | 119 | 3 | 0 | 4 | 122 | 33 | 151 | 2.5e-59 | 229.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48986.t1 | B7FGJ7 | 100.0 | 119 | 0 | 0 | 4 | 122 | 33 | 151 | 8.0e-59 | 235.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053224 | MS.gene48986 | 0.800272 | 1.56E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48986.t1 | MTR_4g024550 | 100.000 | 119 | 0 | 0 | 4 | 122 | 33 | 151 | 1.14e-83 | 241 |
| MS.gene48986.t1 | MTR_5g011910 | 98.319 | 119 | 2 | 0 | 4 | 122 | 33 | 151 | 2.27e-82 | 238 |
| MS.gene48986.t1 | MTR_4g102170 | 96.639 | 119 | 4 | 0 | 4 | 122 | 33 | 151 | 7.19e-81 | 234 |
| MS.gene48986.t1 | MTR_4g024265 | 100.000 | 81 | 0 | 0 | 4 | 84 | 33 | 113 | 4.63e-53 | 171 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48986.t1 | AT4G00100 | 95.798 | 119 | 5 | 0 | 4 | 122 | 33 | 151 | 1.23e-80 | 233 |
| MS.gene48986.t1 | AT3G60770 | 95.798 | 119 | 5 | 0 | 4 | 122 | 33 | 151 | 2.09e-80 | 233 |
Find 32 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCAAATTCAGGCTAATTT+TGG | 0.149134 | 4.3:-74187581 | MS.gene48986:CDS |
| GACCAAAATTAGCCTGAATT+TGG | 0.223891 | 4.3:+74187579 | None:intergenic |
| TATGCGCAAAATCTTGTTTC+CGG | 0.257809 | 4.3:+74187804 | None:intergenic |
| AGATGGAGTTAGACCTTTCT+TGG | 0.271027 | 4.3:+74187882 | None:intergenic |
| TACAGATCTTCAGGAATTTC+AGG | 0.309178 | 4.3:+74187667 | None:intergenic |
| ATCAGGTGATACAGATCTTC+AGG | 0.338551 | 4.3:+74187658 | None:intergenic |
| CAAAGAAGCTTCCACCAGTA+TGG | 0.349985 | 4.3:-74187516 | MS.gene48986:CDS |
| GGTCTAACTCCATCTCAAAT+TGG | 0.359176 | 4.3:-74187874 | MS.gene48986:CDS |
| GGAAGCATTTGGAGAGGAAC+AGG | 0.374872 | 4.3:-74187615 | MS.gene48986:CDS |
| TCAAGAAGGCTGTCTCAATT+AGG | 0.379771 | 4.3:-74187636 | MS.gene48986:CDS |
| AATACTTACTATTTCCATAC+TGG | 0.381602 | 4.3:+74187502 | None:intergenic |
| TTGAGACAGCCTTCTTGATC+AGG | 0.385210 | 4.3:+74187641 | None:intergenic |
| CCATGGAATTGCTCAGGTTA+AGG | 0.405652 | 4.3:-74187833 | MS.gene48986:CDS |
| TGTCTCAATTAGGAAGCATT+TGG | 0.438327 | 4.3:-74187626 | MS.gene48986:CDS |
| AGGACAAGGACTCCAAATTC+AGG | 0.445955 | 4.3:-74187591 | MS.gene48986:CDS |
| ATCTGCAAGTTTGCCAAGAA+AGG | 0.457907 | 4.3:-74187895 | MS.gene48986:CDS |
| GCTCAGGTTAAGGCTGTAAC+CGG | 0.462422 | 4.3:-74187823 | MS.gene48986:CDS |
| TGACTCCCATGGAATTGCTC+AGG | 0.496575 | 4.3:-74187839 | MS.gene48986:CDS |
| GTTATTCTTCGTGACTCCCA+TGG | 0.508409 | 4.3:-74187850 | MS.gene48986:CDS |
| CCTTAACCTGAGCAATTCCA+TGG | 0.520963 | 4.3:+74187833 | None:intergenic |
| TTTCTTGGCAAACTTGCAGA+TGG | 0.531932 | 4.3:+74187897 | None:intergenic |
| TCTTCAAGCAACAAGAGTGC+TGG | 0.541036 | 4.3:+74186905 | None:intergenic |
| ACTTACTATTTCCATACTGG+TGG | 0.562660 | 4.3:+74187505 | None:intergenic |
| TATAGTAACGAGCAAGGCGA+TGG | 0.565886 | 4.3:+74187545 | None:intergenic |
| AGAATAACACCAATTTGAGA+TGG | 0.576179 | 4.3:+74187865 | None:intergenic |
| TCTTCTTATAGTAACGAGCA+AGG | 0.583518 | 4.3:+74187539 | None:intergenic |
| TCTGTATCACCTGATCAAGA+AGG | 0.601322 | 4.3:-74187650 | MS.gene48986:CDS |
| TTGCGCATATTGAAGGCGCA+TGG | 0.612509 | 4.3:-74187790 | MS.gene48986:intron |
| CAATTAGGAAGCATTTGGAG+AGG | 0.640013 | 4.3:-74187621 | MS.gene48986:CDS |
| CTTAACCTGAGCAATTCCAT+GGG | 0.650971 | 4.3:+74187834 | None:intergenic |
| GGAGAGGAACAGGAAGGACA+AGG | 0.660004 | 4.3:-74187605 | MS.gene48986:CDS |
| GCATTTGGAGAGGAACAGGA+AGG | 0.678718 | 4.3:-74187611 | MS.gene48986:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTATCGATTTATT+AGG | - | chr4.3:74187821-74187840 | MS.gene48986:CDS | 10.0% |
| !! | AACATTATAAAGATAAAATG+TGG | + | chr4.3:74188141-74188160 | None:intergenic | 15.0% |
| !! | TATCATTAATATCTTCTTAT+AGG | + | chr4.3:74187016-74187035 | None:intergenic | 15.0% |
| !!! | ATCTCATAAGTAATTATTTT+AGG | - | chr4.3:74188041-74188060 | MS.gene48986:intron | 15.0% |
| !!! | AAGTAAGTTGAAATAAGTTT+AGG | - | chr4.3:74188016-74188035 | MS.gene48986:intron | 20.0% |
| !!! | ACTGAATTTTTTTATTTGCT+TGG | - | chr4.3:74187864-74187883 | MS.gene48986:CDS | 20.0% |
| !!! | ATTTAAAACCAAGAAAATTC+AGG | + | chr4.3:74187899-74187918 | None:intergenic | 20.0% |
| !!! | GTATTCTCTATCTTAATTTT+TGG | - | chr4.3:74188183-74188202 | MS.gene48986:intron | 20.0% |
| !!! | TAAATTTTTGTTCCTGATTA+AGG | - | chr4.3:74188275-74188294 | MS.gene48986:intron | 20.0% |
| !!! | TTTTGTCAATTTTCGTTTTT+TGG | + | chr4.3:74187209-74187228 | None:intergenic | 20.0% |
| ! | AACAATGATATATTCCTGTT+TGG | + | chr4.3:74188072-74188091 | None:intergenic | 25.0% |
| ! | AATACTTACTATTTCCATAC+TGG | + | chr4.3:74187773-74187792 | None:intergenic | 25.0% |
| ! | ATTAGAAATGAGATGCAAAA+TGG | + | chr4.3:74188306-74188325 | None:intergenic | 25.0% |
| ! | TAACAGTATAACACTTAACA+TGG | - | chr4.3:74186912-74186931 | MS.gene48986:CDS | 25.0% |
| ! | TCTCATTTCTAATACTTGTT+TGG | - | chr4.3:74188313-74188332 | MS.gene48986:intron | 25.0% |
| !! | AAGTTCATTAGCTTATAGAA+AGG | + | chr4.3:74187960-74187979 | None:intergenic | 25.0% |
| !!! | CTTTAATTTCTCTTTTCCTA+GGG | - | chr4.3:74187576-74187595 | MS.gene48986:CDS | 25.0% |
| !!! | TCTTTAATTTCTCTTTTCCT+AGG | - | chr4.3:74187575-74187594 | MS.gene48986:CDS | 25.0% |
| !!! | TTTTATTTGCTTGGTTTTGA+AGG | - | chr4.3:74187873-74187892 | MS.gene48986:CDS | 25.0% |
| AAAATGGCAATTCCTTAATC+AGG | + | chr4.3:74188290-74188309 | None:intergenic | 30.0% | |
| AAATGAAGTAACATGTTGCA+AGG | + | chr4.3:74188219-74188238 | None:intergenic | 30.0% | |
| AGAATAACACCAATTTGAGA+TGG | + | chr4.3:74187410-74187429 | None:intergenic | 30.0% | |
| CATTAAAAAAGGTCTGCAAT+TGG | - | chr4.3:74186946-74186965 | MS.gene48986:intron | 30.0% | |
| GGTTGTTTAAACTTATCTCA+TGG | + | chr4.3:74188097-74188116 | None:intergenic | 30.0% | |
| GTGTTTACAGATCATAAAAG+TGG | + | chr4.3:74187263-74187282 | None:intergenic | 30.0% | |
| ! | CCTAGTTAATCTTTTAGATC+CGG | + | chr4.3:74187054-74187073 | None:intergenic | 30.0% |
| !! | TATTTTAGGTAGATCCAAAC+AGG | - | chr4.3:74188055-74188074 | MS.gene48986:intron | 30.0% |
| !! | TTAGGCTTGATTAATTTGTG+AGG | - | chr4.3:74187839-74187858 | MS.gene48986:CDS | 30.0% |
| ACTTACTATTTCCATACTGG+TGG | + | chr4.3:74187770-74187789 | None:intergenic | 35.0% | |
| ATCATCGCATCAAACATGAT+TGG | + | chr4.3:74187096-74187115 | None:intergenic | 35.0% | |
| CCGGATCTAAAAGATTAACT+AGG | - | chr4.3:74187051-74187070 | MS.gene48986:intron | 35.0% | |
| CTGAATTATTGTTGTGATGC+AGG | + | chr4.3:74187138-74187157 | None:intergenic | 35.0% | |
| CTTAATAATGCATAGAGCAG+AGG | + | chr4.3:74188118-74188137 | None:intergenic | 35.0% | |
| GATAATAGTCGATGAACAAC+CGG | - | chr4.3:74187032-74187051 | MS.gene48986:intron | 35.0% | |
| TACAGATCTTCAGGAATTTC+AGG | + | chr4.3:74187608-74187627 | None:intergenic | 35.0% | |
| TCTTCTTATAGTAACGAGCA+AGG | + | chr4.3:74187736-74187755 | None:intergenic | 35.0% | |
| ! | CAAGATTTTGCGCATATTGA+AGG | - | chr4.3:74187475-74187494 | MS.gene48986:intron | 35.0% |
| ! | CTCCAAATTCAGGCTAATTT+TGG | - | chr4.3:74187691-74187710 | MS.gene48986:intron | 35.0% |
| ! | GACCAAAATTAGCCTGAATT+TGG | + | chr4.3:74187696-74187715 | None:intergenic | 35.0% |
| ! | TATGCGCAAAATCTTGTTTC+CGG | + | chr4.3:74187471-74187490 | None:intergenic | 35.0% |
| ! | TTTGAAGGCCTGAATTTTCT+TGG | - | chr4.3:74187888-74187907 | MS.gene48986:CDS | 35.0% |
| !! | TGTCTCAATTAGGAAGCATT+TGG | - | chr4.3:74187646-74187665 | MS.gene48986:CDS | 35.0% |
| AGATGGAGTTAGACCTTTCT+TGG | + | chr4.3:74187393-74187412 | None:intergenic | 40.0% | |
| ATCAGGTGATACAGATCTTC+AGG | + | chr4.3:74187617-74187636 | None:intergenic | 40.0% | |
| ATCTGCAAGTTTGCCAAGAA+AGG | - | chr4.3:74187377-74187396 | MS.gene48986:intron | 40.0% | |
| CTGATGTTCATGAATTGCAC+AGG | - | chr4.3:74187343-74187362 | MS.gene48986:intron | 40.0% | |
| CTTAACCTGAGCAATTCCAT+GGG | + | chr4.3:74187441-74187460 | None:intergenic | 40.0% | |
| GGTCTAACTCCATCTCAAAT+TGG | - | chr4.3:74187398-74187417 | MS.gene48986:intron | 40.0% | |
| TCAAGAAGGCTGTCTCAATT+AGG | - | chr4.3:74187636-74187655 | MS.gene48986:CDS | 40.0% | |
| TCTGTATCACCTGATCAAGA+AGG | - | chr4.3:74187622-74187641 | MS.gene48986:CDS | 40.0% | |
| TTTCTTGGCAAACTTGCAGA+TGG | + | chr4.3:74187378-74187397 | None:intergenic | 40.0% | |
| ! | GTGATGCAGGACAACATTTT+CGG | + | chr4.3:74187125-74187144 | None:intergenic | 40.0% |
| !! | CAATTAGGAAGCATTTGGAG+AGG | - | chr4.3:74187651-74187670 | MS.gene48986:CDS | 40.0% |
| !! | TTGTTGATTCGCTGCAATAG+TGG | + | chr4.3:74188344-74188363 | None:intergenic | 40.0% |
| AGGACAAGGACTCCAAATTC+AGG | - | chr4.3:74187681-74187700 | MS.gene48986:intron | 45.0% | |
| CAAAGAAGCTTCCACCAGTA+TGG | - | chr4.3:74187756-74187775 | MS.gene48986:intron | 45.0% | |
| CCATGGAATTGCTCAGGTTA+AGG | - | chr4.3:74187439-74187458 | MS.gene48986:intron | 45.0% | |
| CCTTAACCTGAGCAATTCCA+TGG | + | chr4.3:74187442-74187461 | None:intergenic | 45.0% | |
| GTTATTCTTCGTGACTCCCA+TGG | - | chr4.3:74187422-74187441 | MS.gene48986:intron | 45.0% | |
| TATAGTAACGAGCAAGGCGA+TGG | + | chr4.3:74187730-74187749 | None:intergenic | 45.0% | |
| TTGAGACAGCCTTCTTGATC+AGG | + | chr4.3:74187634-74187653 | None:intergenic | 45.0% | |
| !!! | TTTTTTTTTTTCATTAAAAA+AGG | - | chr4.3:74186935-74186954 | MS.gene48986:intron | 5.0% |
| GAATTTCAGGAGCAAGCCCT+AGG | + | chr4.3:74187595-74187614 | None:intergenic | 50.0% | |
| GCATTTGGAGAGGAACAGGA+AGG | - | chr4.3:74187661-74187680 | MS.gene48986:CDS | 50.0% | |
| GCTCAGGTTAAGGCTGTAAC+CGG | - | chr4.3:74187449-74187468 | MS.gene48986:intron | 50.0% | |
| TGACTCCCATGGAATTGCTC+AGG | - | chr4.3:74187433-74187452 | MS.gene48986:intron | 50.0% | |
| ! | TTGCGCATATTGAAGGCGCA+TGG | - | chr4.3:74187482-74187501 | MS.gene48986:intron | 50.0% |
| !! | GGAAGCATTTGGAGAGGAAC+AGG | - | chr4.3:74187657-74187676 | MS.gene48986:CDS | 50.0% |
| !! | GGCTAATTTTGGTCGAGAGC+AGG | - | chr4.3:74187702-74187721 | MS.gene48986:intron | 50.0% |
| GGAGAGGAACAGGAAGGACA+AGG | - | chr4.3:74187667-74187686 | MS.gene48986:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 74186908 | 74188386 | 74186908 | ID=MS.gene48986 |
| chr4.3 | mRNA | 74186908 | 74188386 | 74186908 | ID=MS.gene48986.t1;Parent=MS.gene48986 |
| chr4.3 | exon | 74188378 | 74188386 | 74188378 | ID=MS.gene48986.t1.exon1;Parent=MS.gene48986.t1 |
| chr4.3 | CDS | 74188378 | 74188386 | 74188378 | ID=cds.MS.gene48986.t1;Parent=MS.gene48986.t1 |
| chr4.3 | exon | 74187791 | 74187929 | 74187791 | ID=MS.gene48986.t1.exon2;Parent=MS.gene48986.t1 |
| chr4.3 | CDS | 74187791 | 74187929 | 74187791 | ID=cds.MS.gene48986.t1;Parent=MS.gene48986.t1 |
| chr4.3 | exon | 74187511 | 74187697 | 74187511 | ID=MS.gene48986.t1.exon3;Parent=MS.gene48986.t1 |
| chr4.3 | CDS | 74187511 | 74187697 | 74187511 | ID=cds.MS.gene48986.t1;Parent=MS.gene48986.t1 |
| chr4.3 | exon | 74186908 | 74186941 | 74186908 | ID=MS.gene48986.t1.exon4;Parent=MS.gene48986.t1 |
| chr4.3 | CDS | 74186908 | 74186941 | 74186908 | ID=cds.MS.gene48986.t1;Parent=MS.gene48986.t1 |
| Gene Sequence |
| Protein sequence |
