Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49089.t1 | RHN81829.1 | 98.2 | 55 | 0 | 1 | 1 | 54 | 811 | 865 | 3.10E-21 | 110.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49089.t1 | A0A072VNW0 | 98.2 | 55 | 0 | 1 | 1 | 54 | 775 | 829 | 2.3e-21 | 110.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055926 | MS.gene49089 | 0.804231 | 2.39E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene49089 | 0.800982 | 1.12E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49089.t1 | MTR_1g100623 | 98.182 | 55 | 0 | 1 | 1 | 54 | 727 | 781 | 2.43e-31 | 113 |
| MS.gene49089.t1 | MTR_1g100623 | 98.182 | 55 | 0 | 1 | 1 | 54 | 775 | 829 | 2.88e-31 | 113 |
| MS.gene49089.t1 | MTR_1g100607 | 84.211 | 57 | 6 | 2 | 1 | 54 | 153 | 209 | 6.07e-25 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAGGGTTAAGGATTATTA+TGG | 0.244321 | 1.3:-69358931 | MS.gene49089:CDS |
| TTAAGGATTATTATGGATCT+TGG | 0.355305 | 1.3:-69358924 | MS.gene49089:CDS |
| AGGTATGGTTGACAGGGTTA+AGG | 0.362709 | 1.3:-69358941 | None:intergenic |
| TGTGGTGATTGATTCTCCTC+AGG | 0.432207 | 1.3:+69358892 | None:intergenic |
| CAGGAAAAGGTATGGTTGAC+AGG | 0.438569 | 1.3:-69358948 | None:intergenic |
| TAAAGATCATGTTGCAGAAG+TGG | 0.479475 | 1.3:-69358747 | MS.gene49089:CDS |
| GTTGGCAAAGGTGTGGATGA+AGG | 0.542986 | 1.3:-69358719 | MS.gene49089:CDS |
| TGAGGAGAATCAATCACCAC+AGG | 0.595498 | 1.3:-69358890 | MS.gene49089:CDS |
| GCAAAGGTGTGGATGAAGGA+AGG | 0.607651 | 1.3:-69358715 | MS.gene49089:CDS |
| GTTGCAGAAGTGGAACAAGT+TGG | 0.612425 | 1.3:-69358737 | MS.gene49089:CDS |
| ATCTTGGTTTGCTAAACCTG+AGG | 0.638772 | 1.3:-69358908 | MS.gene49089:CDS |
| GAAGTGGAACAAGTTGGCAA+AGG | 0.645524 | 1.3:-69358731 | MS.gene49089:CDS |
| GGAACAAGTTGGCAAAGGTG+TGG | 0.655366 | 1.3:-69358726 | MS.gene49089:CDS |
| AGGAAAAGGTATGGTTGACA+GGG | 0.656335 | 1.3:-69358947 | None:intergenic |
| GAGGAGAATCAATCACCACA+GGG | 0.732593 | 1.3:-69358889 | MS.gene49089:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATGTTGTTATTATACTTAT+AGG | - | chr1.3:69358843-69358862 | MS.gene49089:intron | 15.0% |
| ! | GTTATTATACTTATAGGTGT+AGG | - | chr1.3:69358849-69358868 | MS.gene49089:intron | 25.0% |
| ! | TTAAGGATTATTATGGATCT+TGG | - | chr1.3:69358716-69358735 | MS.gene49089:CDS | 25.0% |
| AGAGAAAAAGAATTACCCTG+TGG | + | chr1.3:69358769-69358788 | None:intergenic | 35.0% | |
| GACAGGGTTAAGGATTATTA+TGG | - | chr1.3:69358709-69358728 | MS.gene49089:CDS | 35.0% | |
| TAAAGATCATGTTGCAGAAG+TGG | - | chr1.3:69358893-69358912 | MS.gene49089:CDS | 35.0% | |
| !! | ATCTTGGTTTGCTAAACCTG+AGG | - | chr1.3:69358732-69358751 | MS.gene49089:CDS | 40.0% |
| GAGGAGAATCAATCACCACA+GGG | - | chr1.3:69358751-69358770 | MS.gene49089:CDS | 45.0% | |
| GTTGCAGAAGTGGAACAAGT+TGG | - | chr1.3:69358903-69358922 | MS.gene49089:CDS | 45.0% | |
| TGAGGAGAATCAATCACCAC+AGG | - | chr1.3:69358750-69358769 | MS.gene49089:CDS | 45.0% | |
| ! | GAAGTGGAACAAGTTGGCAA+AGG | - | chr1.3:69358909-69358928 | MS.gene49089:CDS | 45.0% |
| ! | TGTGGTGATTGATTCTCCTC+AGG | + | chr1.3:69358751-69358770 | None:intergenic | 45.0% |
| ! | GCAAAGGTGTGGATGAAGGA+AGG | - | chr1.3:69358925-69358944 | MS.gene49089:CDS | 50.0% |
| ! | GGAACAAGTTGGCAAAGGTG+TGG | - | chr1.3:69358914-69358933 | MS.gene49089:CDS | 50.0% |
| ! | GTTGGCAAAGGTGTGGATGA+AGG | - | chr1.3:69358921-69358940 | MS.gene49089:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 69358703 | 69358959 | 69358703 | ID=MS.gene49089 |
| chr1.3 | mRNA | 69358703 | 69358959 | 69358703 | ID=MS.gene49089.t1;Parent=MS.gene49089 |
| chr1.3 | exon | 69358890 | 69358959 | 69358890 | ID=MS.gene49089.t1.exon1;Parent=MS.gene49089.t1 |
| chr1.3 | CDS | 69358890 | 69358959 | 69358890 | ID=cds.MS.gene49089.t1;Parent=MS.gene49089.t1 |
| chr1.3 | exon | 69358703 | 69358797 | 69358703 | ID=MS.gene49089.t1.exon2;Parent=MS.gene49089.t1 |
| chr1.3 | CDS | 69358703 | 69358797 | 69358703 | ID=cds.MS.gene49089.t1;Parent=MS.gene49089.t1 |
| Gene Sequence |
| Protein sequence |
