Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49156.t1 | XP_003592191.1 | 84.6 | 136 | 19 | 2 | 1 | 134 | 1 | 136 | 2.00E-40 | 175.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49156.t1 | Q9LTE3 | 55.4 | 74 | 32 | 1 | 1 | 74 | 1 | 73 | 6.0e-14 | 78.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49156.t1 | G7IDY9 | 84.6 | 136 | 19 | 2 | 1 | 134 | 1 | 136 | 1.4e-40 | 175.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05861 | MS.gene49156 | 0.801693 | 7.98E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49156.t1 | MTR_1g099800 | 89.706 | 136 | 12 | 2 | 1 | 134 | 1 | 136 | 4.68e-67 | 199 |
| MS.gene49156.t1 | MTR_1g099815 | 86.567 | 134 | 16 | 1 | 1 | 134 | 1 | 132 | 1.67e-61 | 185 |
| MS.gene49156.t1 | MTR_1g102240 | 86.567 | 134 | 16 | 1 | 1 | 134 | 1 | 132 | 7.47e-61 | 183 |
| MS.gene49156.t1 | MTR_1g099810 | 77.857 | 140 | 24 | 5 | 1 | 134 | 1 | 139 | 4.71e-51 | 159 |
| MS.gene49156.t1 | MTR_1g099810 | 76.860 | 121 | 21 | 5 | 20 | 134 | 1 | 120 | 3.19e-39 | 128 |
| MS.gene49156.t1 | MTR_6g049280 | 57.037 | 135 | 44 | 4 | 1 | 133 | 31 | 153 | 2.68e-34 | 117 |
| MS.gene49156.t1 | MTR_6g049280 | 57.778 | 135 | 43 | 4 | 1 | 133 | 1 | 123 | 3.63e-34 | 115 |
| MS.gene49156.t1 | MTR_6g051690 | 55.556 | 135 | 49 | 4 | 1 | 133 | 1 | 126 | 1.03e-32 | 112 |
| MS.gene49156.t1 | MTR_6g049320 | 56.296 | 135 | 45 | 4 | 1 | 133 | 31 | 153 | 1.16e-32 | 112 |
| MS.gene49156.t1 | MTR_6g049320 | 57.037 | 135 | 44 | 4 | 1 | 133 | 1 | 123 | 1.50e-32 | 111 |
| MS.gene49156.t1 | MTR_1g099810 | 75.962 | 104 | 19 | 4 | 36 | 134 | 2 | 104 | 2.63e-30 | 105 |
| MS.gene49156.t1 | MTR_8g098915 | 48.175 | 137 | 56 | 4 | 1 | 133 | 1 | 126 | 3.75e-30 | 105 |
| MS.gene49156.t1 | MTR_6g051680 | 70.000 | 70 | 20 | 1 | 1 | 69 | 1 | 70 | 2.29e-29 | 103 |
| MS.gene49156.t1 | MTR_1g099640 | 55.224 | 134 | 54 | 3 | 1 | 133 | 1 | 129 | 9.38e-29 | 102 |
| MS.gene49156.t1 | MTR_3g434890 | 44.715 | 123 | 49 | 4 | 1 | 119 | 1 | 108 | 1.91e-26 | 95.9 |
| MS.gene49156.t1 | MTR_7g451210 | 62.667 | 75 | 27 | 1 | 1 | 74 | 1 | 75 | 2.53e-26 | 95.5 |
| MS.gene49156.t1 | MTR_3g434960 | 44.715 | 123 | 49 | 4 | 1 | 119 | 1 | 108 | 3.33e-26 | 95.1 |
| MS.gene49156.t1 | MTR_7g451200 | 43.902 | 123 | 55 | 2 | 1 | 122 | 1 | 110 | 3.48e-26 | 95.1 |
| MS.gene49156.t1 | MTR_0795s0020 | 53.237 | 139 | 46 | 5 | 1 | 133 | 1 | 126 | 3.53e-26 | 95.5 |
| MS.gene49156.t1 | MTR_1173s0010 | 59.740 | 77 | 30 | 1 | 1 | 76 | 1 | 77 | 1.20e-25 | 93.6 |
| MS.gene49156.t1 | MTR_6g049260 | 59.211 | 76 | 30 | 1 | 1 | 75 | 1 | 76 | 1.60e-25 | 93.6 |
| MS.gene49156.t1 | MTR_3g435000 | 57.746 | 71 | 29 | 1 | 6 | 75 | 10 | 80 | 7.27e-23 | 86.7 |
| MS.gene49156.t1 | MTR_8g098915 | 44.068 | 118 | 51 | 4 | 20 | 133 | 1 | 107 | 7.29e-20 | 79.0 |
| MS.gene49156.t1 | MTR_7g079110 | 57.353 | 68 | 28 | 1 | 1 | 67 | 1 | 68 | 1.96e-18 | 77.0 |
| MS.gene49156.t1 | MTR_3g434920 | 42.708 | 96 | 36 | 4 | 28 | 119 | 2 | 82 | 9.12e-15 | 65.1 |
| MS.gene49156.t1 | MTR_1g099640 | 51.020 | 98 | 43 | 2 | 36 | 133 | 2 | 94 | 3.48e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49156.t1 | AT5G52760 | 41.429 | 140 | 58 | 6 | 2 | 134 | 4 | 126 | 2.95e-20 | 80.5 |
| MS.gene49156.t1 | AT5G52740 | 55.072 | 69 | 30 | 1 | 1 | 69 | 1 | 68 | 2.65e-19 | 77.8 |
| MS.gene49156.t1 | AT1G01490 | 56.338 | 71 | 29 | 2 | 1 | 69 | 1 | 71 | 3.17e-17 | 73.9 |
| MS.gene49156.t1 | AT1G01490 | 56.338 | 71 | 29 | 2 | 1 | 69 | 1 | 71 | 3.17e-17 | 73.9 |
| MS.gene49156.t1 | AT1G01490 | 55.072 | 69 | 29 | 2 | 3 | 69 | 30 | 98 | 1.45e-15 | 70.1 |
| MS.gene49156.t1 | AT5G52750 | 50.769 | 65 | 31 | 1 | 3 | 66 | 5 | 69 | 7.79e-14 | 64.3 |
Find 23 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGCAGTCTCTGGCCTTTC+AGG | 0.156873 | 1.3:-68709583 | MS.gene49156:intron |
| ATTAATCCTTTCGTGCTCTA+TGG | 0.313770 | 1.3:-68709142 | MS.gene49156:CDS |
| ATTCATCTGATGATTATAAT+AGG | 0.369814 | 1.3:+68709108 | None:intergenic |
| ACAGAAAATTGTGCTAAAGT+TGG | 0.399453 | 1.3:-68709641 | MS.gene49156:intron |
| ACGAAAGGATTAATTATCAT+TGG | 0.419156 | 1.3:+68709151 | None:intergenic |
| AGCTTAGCCACTACAAGTAC+TGG | 0.428511 | 1.3:+68709283 | None:intergenic |
| TATATGATGACACAGCCATT+AGG | 0.436801 | 1.3:+68709043 | None:intergenic |
| AGGTTCCTCTTCAACACTTA+CGG | 0.439137 | 1.3:+68709063 | None:intergenic |
| TGGGTCGGTATCTCCTACTA+AGG | 0.446407 | 1.3:+68709303 | None:intergenic |
| GACAAGAAAATGACCTTAGT+AGG | 0.452020 | 1.3:-68709316 | MS.gene49156:CDS |
| AAAGCTATGAAGGCAGTCTC+TGG | 0.474866 | 1.3:-68709592 | MS.gene49156:CDS |
| TTCAGTTGGACCAGCTAAAG+AGG | 0.475094 | 1.3:-68709233 | MS.gene49156:CDS |
| GCACGGTAGTATGAATTGTA+TGG | 0.489699 | 1.3:+68709085 | None:intergenic |
| TGATGATTATAATAGGTAGT+AGG | 0.521368 | 1.3:+68709115 | None:intergenic |
| CATGCAGAAATACTTTCAGT+TGG | 0.545738 | 1.3:-68709247 | MS.gene49156:CDS |
| TACTTGTAGTGGCTAAGCTA+AGG | 0.547972 | 1.3:-68709279 | MS.gene49156:CDS |
| CCGTGCCGTAAGTGTTGAAG+AGG | 0.556050 | 1.3:-68709068 | MS.gene49156:CDS |
| AGTGTTGAAGAGGAACCTAA+TGG | 0.587634 | 1.3:-68709058 | MS.gene49156:CDS |
| GGTGTACCATAGAGCACGAA+AGG | 0.612408 | 1.3:+68709136 | None:intergenic |
| CCTCTTCAACACTTACGGCA+CGG | 0.624516 | 1.3:+68709068 | None:intergenic |
| GCTTAGCCACTACAAGTACT+GGG | 0.632143 | 1.3:+68709284 | None:intergenic |
| AGCCACTACAAGTACTGGGT+CGG | 0.650008 | 1.3:+68709288 | None:intergenic |
| TACCGACCCAGTACTTGTAG+TGG | 0.655849 | 1.3:-68709290 | MS.gene49156:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAAGATGATAAAAAGA+AGG | - | chr1.3:68709689-68709708 | MS.gene49156:intron | 15.0% |
| !!! | TGTATTTTAATAATTTTGCA+GGG | - | chr1.3:68709520-68709539 | MS.gene49156:intron | 15.0% |
| !!! | TTCTTTTTATCATCTTTTTT+AGG | + | chr1.3:68709690-68709709 | None:intergenic | 15.0% |
| !!! | TTGTATTTTAATAATTTTGC+AGG | - | chr1.3:68709519-68709538 | MS.gene49156:intron | 15.0% |
| !! | ATTCATCTGATGATTATAAT+AGG | + | chr1.3:68709778-68709797 | None:intergenic | 20.0% |
| !!! | AAAAAATCTACTTGTTAGAA+CGG | + | chr1.3:68709217-68709236 | None:intergenic | 20.0% |
| !!! | GTATTTTAATAATTTTGCAG+GGG | - | chr1.3:68709521-68709540 | MS.gene49156:intron | 20.0% |
| ! | AATCAAACAAAAAGCTATGA+AGG | - | chr1.3:68709281-68709300 | MS.gene49156:CDS | 25.0% |
| ! | ACGAAAGGATTAATTATCAT+TGG | + | chr1.3:68709735-68709754 | None:intergenic | 25.0% |
| ! | ATCGAACTTAGTCTAATATA+CGG | + | chr1.3:68709408-68709427 | None:intergenic | 25.0% |
| ! | TATCATAGCTAAAGTATGAT+TGG | - | chr1.3:68709094-68709113 | MS.gene49156:CDS | 25.0% |
| ! | TCATACTTTAGCTATGATAT+AGG | + | chr1.3:68709093-68709112 | None:intergenic | 25.0% |
| ! | TGATGATTATAATAGGTAGT+AGG | + | chr1.3:68709771-68709790 | None:intergenic | 25.0% |
| ! | TTGAAATTGTGCATAAGAAA+AGG | + | chr1.3:68709128-68709147 | None:intergenic | 25.0% |
| !!! | AATCATTGATTCTACGAATT+TGG | + | chr1.3:68709464-68709483 | None:intergenic | 25.0% |
| !!! | ACTAAGTTCGATATTTTGAT+TGG | - | chr1.3:68709416-68709435 | MS.gene49156:intron | 25.0% |
| !!! | ATTTTTTTCCTTTACCTGAA+AGG | + | chr1.3:68709317-68709336 | None:intergenic | 25.0% |
| !!! | TAAAAGAGTGGTTTTAATCA+AGG | + | chr1.3:68709067-68709086 | None:intergenic | 25.0% |
| ACAGAAAATTGTGCTAAAGT+TGG | - | chr1.3:68709242-68709261 | MS.gene49156:CDS | 30.0% | |
| TGATATAGGAGATAAAAGAG+TGG | + | chr1.3:68709079-68709098 | None:intergenic | 30.0% | |
| ATTAATCCTTTCGTGCTCTA+TGG | - | chr1.3:68709741-68709760 | MS.gene49156:intron | 35.0% | |
| CATGCAGAAATACTTTCAGT+TGG | - | chr1.3:68709636-68709655 | MS.gene49156:CDS | 35.0% | |
| GACAAGAAAATGACCTTAGT+AGG | - | chr1.3:68709567-68709586 | MS.gene49156:intron | 35.0% | |
| TATATGATGACACAGCCATT+AGG | + | chr1.3:68709843-68709862 | None:intergenic | 35.0% | |
| TTGATTGGACAATTCAGATG+TGG | - | chr1.3:68709431-68709450 | MS.gene49156:intron | 35.0% | |
| ! | TACCAAAGTGCTTCAAATTC+AGG | + | chr1.3:68709152-68709171 | None:intergenic | 35.0% |
| ! | TCTTTCTTTTCCTCTTTAGC+TGG | + | chr1.3:68709663-68709682 | None:intergenic | 35.0% |
| AGGTTCCTCTTCAACACTTA+CGG | + | chr1.3:68709823-68709842 | None:intergenic | 40.0% | |
| GCACGGTAGTATGAATTGTA+TGG | + | chr1.3:68709801-68709820 | None:intergenic | 40.0% | |
| TACTTGTAGTGGCTAAGCTA+AGG | - | chr1.3:68709604-68709623 | MS.gene49156:CDS | 40.0% | |
| ! | AGCCTGAATTTGAAGCACTT+TGG | - | chr1.3:68709147-68709166 | MS.gene49156:CDS | 40.0% |
| ! | AGTGTTGAAGAGGAACCTAA+TGG | - | chr1.3:68709825-68709844 | MS.gene49156:intron | 40.0% |
| AAAGCTATGAAGGCAGTCTC+TGG | - | chr1.3:68709291-68709310 | MS.gene49156:CDS | 45.0% | |
| AGCTTAGCCACTACAAGTAC+TGG | + | chr1.3:68709603-68709622 | None:intergenic | 45.0% | |
| GCTTAGCCACTACAAGTACT+GGG | + | chr1.3:68709602-68709621 | None:intergenic | 45.0% | |
| TTCAGTTGGACCAGCTAAAG+AGG | - | chr1.3:68709650-68709669 | MS.gene49156:intron | 45.0% | |
| CCTCTTCAACACTTACGGCA+CGG | + | chr1.3:68709818-68709837 | None:intergenic | 50.0% | |
| GGTGTACCATAGAGCACGAA+AGG | + | chr1.3:68709750-68709769 | None:intergenic | 50.0% | |
| TACCGACCCAGTACTTGTAG+TGG | - | chr1.3:68709593-68709612 | MS.gene49156:CDS | 50.0% | |
| TGGGTCGGTATCTCCTACTA+AGG | + | chr1.3:68709583-68709602 | None:intergenic | 50.0% | |
| ! | GTCTCTGGCCTTTCAGGTAA+AGG | - | chr1.3:68709306-68709325 | MS.gene49156:CDS | 50.0% |
| !! | AGCCACTACAAGTACTGGGT+CGG | + | chr1.3:68709598-68709617 | None:intergenic | 50.0% |
| ! | AAGGCAGTCTCTGGCCTTTC+AGG | - | chr1.3:68709300-68709319 | MS.gene49156:CDS | 55.0% |
| !! | CCGTGCCGTAAGTGTTGAAG+AGG | - | chr1.3:68709815-68709834 | MS.gene49156:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 68709042 | 68709863 | 68709042 | ID=MS.gene49156 |
| chr1.3 | mRNA | 68709042 | 68709863 | 68709042 | ID=MS.gene49156.t1;Parent=MS.gene49156 |
| chr1.3 | exon | 68709858 | 68709863 | 68709858 | ID=MS.gene49156.t1.exon1;Parent=MS.gene49156.t1 |
| chr1.3 | CDS | 68709858 | 68709863 | 68709858 | ID=cds.MS.gene49156.t1;Parent=MS.gene49156.t1 |
| chr1.3 | exon | 68709584 | 68709659 | 68709584 | ID=MS.gene49156.t1.exon2;Parent=MS.gene49156.t1 |
| chr1.3 | CDS | 68709584 | 68709659 | 68709584 | ID=cds.MS.gene49156.t1;Parent=MS.gene49156.t1 |
| chr1.3 | exon | 68709042 | 68709364 | 68709042 | ID=MS.gene49156.t1.exon3;Parent=MS.gene49156.t1 |
| chr1.3 | CDS | 68709042 | 68709364 | 68709042 | ID=cds.MS.gene49156.t1;Parent=MS.gene49156.t1 |
| Gene Sequence |
| Protein sequence |
