Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49175.t1 | XP_019438836.1 | 63.5 | 63 | 23 | 0 | 51 | 113 | 141 | 203 | 8.30E-16 | 93.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49175.t1 | A0A445GI25 | 68.3 | 63 | 17 | 1 | 50 | 109 | 113 | 175 | 2.1e-13 | 84.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051125 | MS.gene49175 | 0.804908 | 1.72E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTTCACTCTTCAAAA+TGG | 0.170935 | 1.3:-68485626 | None:intergenic |
| AATTTGAACTCAACAGGAAA+TGG | 0.399868 | 1.3:+68485155 | MS.gene49175:CDS |
| CATCTGAATTTGAACTCAAC+AGG | 0.409257 | 1.3:+68485149 | MS.gene49175:CDS |
| AAGATCTTACATTCATCATC+AGG | 0.417940 | 1.3:-68485497 | None:intergenic |
| TCAGCTTCACTCTTCAAAAT+GGG | 0.432393 | 1.3:-68485625 | None:intergenic |
| GAGTGAGACCAGTAGTAGTT+TGG | 0.451269 | 1.3:-68485233 | None:intergenic |
| CTCATCGTAGTCGACTTCAT+TGG | 0.458097 | 1.3:+68485257 | MS.gene49175:CDS |
| CTATGGAGCAAGGAGAATAA+TGG | 0.470206 | 1.3:+68485316 | MS.gene49175:CDS |
| GGATAATGTTGGTAATTCTC+AGG | 0.478704 | 1.3:+68485182 | MS.gene49175:CDS |
| CTTTGATGTGTGCTAGAAGT+TGG | 0.519354 | 1.3:+68485293 | MS.gene49175:CDS |
| ACTTCATTGGACAACCTTGG+AGG | 0.521822 | 1.3:+68485270 | MS.gene49175:CDS |
| AGTGCACACCAAACTACTAC+TGG | 0.530092 | 1.3:+68485225 | MS.gene49175:CDS |
| TCGACTTCATTGGACAACCT+TGG | 0.544964 | 1.3:+68485267 | MS.gene49175:CDS |
| TAGAAGTTGGCTATGGAGCA+AGG | 0.597997 | 1.3:+68485306 | MS.gene49175:CDS |
| GAAATGGACATGGATAATGT+TGG | 0.622790 | 1.3:+68485171 | MS.gene49175:CDS |
| AACTCAACAGGAAATGGACA+TGG | 0.624712 | 1.3:+68485161 | MS.gene49175:CDS |
| AGCACACATCAAAGCCTCCA+AGG | 0.630649 | 1.3:-68485284 | None:intergenic |
| CAATGAAGTCGACTACGATG+AGG | 0.638748 | 1.3:-68485256 | None:intergenic |
| TGTGTGCTAGAAGTTGGCTA+TGG | 0.638996 | 1.3:+68485299 | MS.gene49175:CDS |
| AATGAAGTCGACTACGATGA+GGG | 0.641996 | 1.3:-68485255 | None:intergenic |
| ATAGGTGAAATATTGACTAG+TGG | 0.646439 | 1.3:+68485594 | MS.gene49175:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTGTATTTTTTATTATAT+AGG | + | chr1.3:68485576-68485595 | MS.gene49175:intron | 10.0% |
| !! | AAACATTAAAGTTAGTAAAT+TGG | - | chr1.3:68485548-68485567 | None:intergenic | 15.0% |
| !! | ATTAAAGTTAGTAAATTGGA+AGG | - | chr1.3:68485544-68485563 | None:intergenic | 20.0% |
| !! | CTTTCAATTTACATATCTTA+AGG | + | chr1.3:68485356-68485375 | MS.gene49175:intron | 20.0% |
| ! | GGAAGGTAGTATTAAATAAA+TGG | - | chr1.3:68485527-68485546 | None:intergenic | 25.0% |
| AAGATCTTACATTCATCATC+AGG | - | chr1.3:68485500-68485519 | None:intergenic | 30.0% | |
| AATTTGAACTCAACAGGAAA+TGG | + | chr1.3:68485155-68485174 | MS.gene49175:CDS | 30.0% | |
| ACTGCATTTGCATTATTTAC+AGG | + | chr1.3:68485419-68485438 | MS.gene49175:intron | 30.0% | |
| ! | ATAGGTGAAATATTGACTAG+TGG | + | chr1.3:68485594-68485613 | MS.gene49175:intron | 30.0% |
| CATCTGAATTTGAACTCAAC+AGG | + | chr1.3:68485149-68485168 | MS.gene49175:CDS | 35.0% | |
| GAAATGGACATGGATAATGT+TGG | + | chr1.3:68485171-68485190 | MS.gene49175:CDS | 35.0% | |
| !! | GGATAATGTTGGTAATTCTC+AGG | + | chr1.3:68485182-68485201 | MS.gene49175:CDS | 35.0% |
| AACTCAACAGGAAATGGACA+TGG | + | chr1.3:68485161-68485180 | MS.gene49175:CDS | 40.0% | |
| AATGAAGTCGACTACGATGA+GGG | - | chr1.3:68485258-68485277 | None:intergenic | 40.0% | |
| CTATGGAGCAAGGAGAATAA+TGG | + | chr1.3:68485316-68485335 | MS.gene49175:CDS | 40.0% | |
| CTTTGATGTGTGCTAGAAGT+TGG | + | chr1.3:68485293-68485312 | MS.gene49175:CDS | 40.0% | |
| ACTTCATTGGACAACCTTGG+AGG | + | chr1.3:68485270-68485289 | MS.gene49175:CDS | 45.0% | |
| AGTGCACACCAAACTACTAC+TGG | + | chr1.3:68485225-68485244 | MS.gene49175:CDS | 45.0% | |
| CAATGAAGTCGACTACGATG+AGG | - | chr1.3:68485259-68485278 | None:intergenic | 45.0% | |
| GAGTGAGACCAGTAGTAGTT+TGG | - | chr1.3:68485236-68485255 | None:intergenic | 45.0% | |
| TAGAAGTTGGCTATGGAGCA+AGG | + | chr1.3:68485306-68485325 | MS.gene49175:CDS | 45.0% | |
| TCGACTTCATTGGACAACCT+TGG | + | chr1.3:68485267-68485286 | MS.gene49175:CDS | 45.0% | |
| TGTGTGCTAGAAGTTGGCTA+TGG | + | chr1.3:68485299-68485318 | MS.gene49175:CDS | 45.0% | |
| ! | CTCATCGTAGTCGACTTCAT+TGG | + | chr1.3:68485257-68485276 | MS.gene49175:CDS | 45.0% |
| AGCACACATCAAAGCCTCCA+AGG | - | chr1.3:68485287-68485306 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 68485109 | 68485641 | 68485109 | ID=MS.gene49175 |
| chr1.3 | mRNA | 68485109 | 68485641 | 68485109 | ID=MS.gene49175.t1;Parent=MS.gene49175 |
| chr1.3 | exon | 68485109 | 68485337 | 68485109 | ID=MS.gene49175.t1.exon1;Parent=MS.gene49175.t1 |
| chr1.3 | CDS | 68485109 | 68485337 | 68485109 | ID=cds.MS.gene49175.t1;Parent=MS.gene49175.t1 |
| chr1.3 | exon | 68485441 | 68485509 | 68485441 | ID=MS.gene49175.t1.exon2;Parent=MS.gene49175.t1 |
| chr1.3 | CDS | 68485441 | 68485509 | 68485441 | ID=cds.MS.gene49175.t1;Parent=MS.gene49175.t1 |
| chr1.3 | exon | 68485598 | 68485641 | 68485598 | ID=MS.gene49175.t1.exon3;Parent=MS.gene49175.t1 |
| chr1.3 | CDS | 68485598 | 68485641 | 68485598 | ID=cds.MS.gene49175.t1;Parent=MS.gene49175.t1 |
| Gene Sequence |
| Protein sequence |
