AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49445

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49445.t1 MTR_1g094930 99.094 331 3 0 1 331 1 331 0.0 675
MS.gene49445.t1 MTR_1g094930 99.153 236 2 0 96 331 1 236 2.00e-174 483
MS.gene49445.t1 MTR_1g116010 55.814 258 78 10 74 329 22 245 7.15e-74 228
MS.gene49445.t1 MTR_2g094600 46.774 186 70 6 121 306 124 280 5.12e-43 150
MS.gene49445.t1 MTR_1g006650 34.783 322 133 11 9 306 30 298 4.70e-38 139
MS.gene49445.t1 MTR_3g109760 34.228 298 134 8 17 306 28 271 1.36e-36 134
MS.gene49445.t1 MTR_7g085410 35.938 256 129 6 58 306 25 252 3.86e-35 129
MS.gene49445.t1 MTR_4g058910 64.894 94 28 1 238 331 310 398 3.92e-35 132
MS.gene49445.t1 MTR_4g071590 57.692 104 33 2 203 306 183 275 1.83e-34 128
MS.gene49445.t1 MTR_4g471590 57.692 104 33 2 203 306 183 275 1.83e-34 128
MS.gene49445.t1 MTR_6g032830 36.015 261 122 9 67 308 24 258 2.01e-34 127
MS.gene49445.t1 MTR_5g021340 35.766 274 121 10 48 308 135 366 9.69e-34 128
MS.gene49445.t1 MTR_3g117380 72.603 73 19 1 234 306 222 293 6.50e-33 124
MS.gene49445.t1 MTR_8g013950 81.538 65 12 0 242 306 226 290 9.46e-33 124
MS.gene49445.t1 MTR_5g007600 55.238 105 45 2 203 306 220 323 1.64e-31 120
MS.gene49445.t1 MTR_5g007600 54.000 100 44 2 207 305 122 220 2.11e-28 112
MS.gene49445.t1 MTR_8g076340 62.366 93 26 3 216 308 201 284 1.97e-31 119
MS.gene49445.t1 MTR_1g046680 80.000 65 13 0 242 306 172 236 2.15e-31 118
MS.gene49445.t1 MTR_7g446110 61.290 62 24 0 242 303 187 248 1.42e-20 89.7
MS.gene49445.t1 MTR_7g445950 58.065 62 26 0 242 303 120 181 2.61e-19 84.3
MS.gene49445.t1 MTR_7g445980 61.667 60 22 1 246 304 37 96 7.28e-19 80.9
MS.gene49445.t1 MTR_7g446070 36.567 134 65 2 175 303 75 193 8.03e-19 83.6
MS.gene49445.t1 MTR_7g106090 55.844 77 33 1 177 253 36 111 7.41e-17 75.9
MS.gene49445.t1 MTR_5g020230 52.083 48 23 0 227 274 107 154 3.04e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49445.t1 AT1G08010 40.254 236 111 5 85 318 89 296 2.89e-42 149
MS.gene49445.t1 AT1G08010 40.254 236 111 5 85 318 89 296 2.89e-42 149
MS.gene49445.t1 AT1G08010 40.254 236 111 5 85 318 89 296 2.89e-42 149
MS.gene49445.t1 AT3G54810 71.875 96 20 2 240 328 227 322 3.09e-42 149
MS.gene49445.t1 AT3G54810 71.875 96 20 2 240 328 227 322 3.09e-42 149
MS.gene49445.t1 AT5G25830 40.249 241 113 8 67 306 73 283 6.84e-38 138
MS.gene49445.t1 AT1G08000 42.051 195 88 3 115 309 116 285 3.01e-37 135
MS.gene49445.t1 AT1G08000 42.051 195 88 3 115 309 116 285 3.01e-37 135
MS.gene49445.t1 AT4G32890 34.641 306 120 12 17 306 20 261 7.49e-37 134
MS.gene49445.t1 AT5G66320 38.636 264 123 9 55 315 95 322 2.51e-36 134
MS.gene49445.t1 AT5G66320 38.636 264 123 9 55 315 95 322 2.51e-36 134
MS.gene49445.t1 AT3G24050 78.462 65 14 0 242 306 194 258 1.03e-31 120
MS.gene49445.t1 AT3G51080 71.053 76 21 1 242 317 221 295 1.09e-31 121
MS.gene49445.t1 AT4G36240 54.545 99 43 1 208 306 132 228 3.08e-31 118
MS.gene49445.t1 AT3G60530 74.242 66 17 0 241 306 157 222 9.78e-31 116
MS.gene49445.t1 AT2G28340 65.333 75 26 0 244 318 193 267 1.31e-30 117
MS.gene49445.t1 AT2G45050 72.727 66 18 0 241 306 178 243 3.21e-30 115
MS.gene49445.t1 AT4G34680 63.158 76 28 0 242 317 180 255 5.77e-29 112
MS.gene49445.t1 AT4G34680 63.158 76 28 0 242 317 180 255 5.77e-29 112
MS.gene49445.t1 AT3G45170 40.000 140 79 3 168 306 44 179 4.68e-28 108
MS.gene49445.t1 AT5G26930 54.545 44 18 1 234 277 20 61 4.90e-11 59.7

Find 106 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGCAGCAGGCCGGTATTC+GGG 0.281895 1.1:-68332809 None:intergenic
ACAACTCGTTGGCGAGTATC+TGG 0.294745 1.1:+68330564 MS.gene49445:CDS
AAATGAATCGTCCACAATAT+TGG 0.301923 1.1:-68332216 None:intergenic
GTTCGAAGATCTTCTTTCAT+TGG 0.321117 1.1:+68332184 MS.gene49445:intron
CTGCTTTCAAGGACAGAAAT+AGG 0.322926 1.1:-68332335 None:intergenic
GTCTCTCCAGAAGAGAGCTT+TGG 0.342561 1.1:-68332593 None:intergenic
CAATGCATGCATTTCCTAAC+AGG 0.350621 1.1:-68332689 None:intergenic
CGTCCGCAAGGCACCGGAAT+GGG 0.362306 1.1:-68332398 None:intergenic
ACTAGCAGCAGGCCGGTATT+CGG 0.363594 1.1:-68332810 None:intergenic
TCTCTACTCACCGGATCAAC+GGG 0.368770 1.1:-68330647 None:intergenic
GGCTGTGGGGCGTGGACGCT+TGG 0.384746 1.1:-68332429 None:intergenic
AAGGCGAGAACATTGGTGTT+TGG 0.385415 1.1:-68332929 None:intergenic
TATCCTTGCATCTCATTTCA+AGG 0.385918 1.1:-68332877 None:intergenic
CAATGGAGGGCAGGTCCAAT+GGG 0.390212 1.1:+68332731 MS.gene49445:CDS
AGCAGGAACTACTGCTTTCA+AGG 0.398558 1.1:-68332346 None:intergenic
GCACTCCAATTCGCACAAAA+AGG 0.399415 1.1:+68332853 MS.gene49445:CDS
ACAATGGAGGGCAGGTCCAA+TGG 0.404651 1.1:+68332730 MS.gene49445:CDS
CCTTGGAATACAACATTTGA+AGG 0.412271 1.1:+68332948 MS.gene49445:CDS
TTGGTTTCTTGACAATAATC+CGG 0.416836 1.1:-68332574 None:intergenic
GGGTTGAAGGCTGTGGGGCG+TGG 0.418136 1.1:-68332437 None:intergenic
TGCCCTCCATTGTGGAGTCT+TGG 0.422561 1.1:-68332720 None:intergenic
AAGAAACCAAAGCTCTCTTC+TGG 0.429167 1.1:+68332587 MS.gene49445:CDS
CCCTACTTCCTCTTCTGTTG+AGG 0.447338 1.1:+68332484 MS.gene49445:CDS
TTCTGTTCGCCACTGTCAGC+TGG 0.447903 1.1:-68332656 None:intergenic
AGAATGGTTCACTACCTGTT+AGG 0.449879 1.1:+68332675 MS.gene49445:CDS
GCTGTCAGCAAGGAGGACTC+TGG 0.452116 1.1:+68332287 MS.gene49445:CDS
ATCTGAAGAAAGCGCCTTGG+TGG 0.456913 1.1:-68332531 None:intergenic
TGCGAATTGGAGTGCACAGC+TGG 0.458877 1.1:-68332845 None:intergenic
GGCGGAGCTCCCCGTTGATC+CGG 0.465097 1.1:+68330637 MS.gene49445:CDS
TGGTTTCTTGACAATAATCC+GGG 0.466726 1.1:-68332573 None:intergenic
TGCTCTGGTGGAAAGACCAC+AGG 0.471537 1.1:+68332365 MS.gene49445:CDS
TGCGGTGTACGCTACAAGTC+GGG 0.473402 1.1:+68332776 MS.gene49445:CDS
GCGGTGTCAACGTCGTCGAC+GGG 0.476293 1.1:-68330509 None:intergenic
CCTTCAAATGTTGTATTCCA+AGG 0.482354 1.1:-68332948 None:intergenic
AGCGGTGTCAACGTCGTCGA+CGG 0.482359 1.1:-68330510 None:intergenic
GTTGTAGTTTGCCGCGGAGG+AGG 0.484221 1.1:-68330546 None:intergenic
GCCGACTATTCCCCGAATAC+CGG 0.484840 1.1:+68332798 MS.gene49445:CDS
CTCTCTACTCACCGGATCAA+CGG 0.485631 1.1:-68330648 None:intergenic
AGATCTTCTTTCATTGGAGT+GGG 0.485791 1.1:+68332190 MS.gene49445:CDS
AAGATCTTCTTTCATTGGAG+TGG 0.486353 1.1:+68332189 MS.gene49445:CDS
CCTCAACAGAAGAGGAAGTA+GGG 0.487341 1.1:-68332484 None:intergenic
AGAACGAGGGTTGAAGGCTG+TGG 0.494477 1.1:-68332444 None:intergenic
AAACACCAATGTTCTCGCCT+TGG 0.495323 1.1:+68332931 MS.gene49445:CDS
CATAGCAGAACGAGGGTTGA+AGG 0.496157 1.1:-68332450 None:intergenic
CGTGCACGTCCGCAAGGCAC+CGG 0.497396 1.1:-68332404 None:intergenic
AGGGATTTACGTCCCCATTC+CGG 0.508516 1.1:+68332385 MS.gene49445:CDS
GCCTTGGTGGAGATAACATT+AGG 0.511767 1.1:-68332518 None:intergenic
GAAAGCAGTAGTTCCTGCTC+TGG 0.513297 1.1:+68332350 MS.gene49445:CDS
TGTGGTCTTTCCACCAGAGC+AGG 0.513350 1.1:-68332363 None:intergenic
GAACGAGGGTTGAAGGCTGT+GGG 0.515671 1.1:-68332443 None:intergenic
ATTGGACCTGCCCTCCATTG+TGG 0.524395 1.1:-68332728 None:intergenic
GGAGGGCAATGAAGCGGCAG+CGG 0.526729 1.1:-68330528 None:intergenic
GCCGGTATTCGGGGAATAGT+CGG 0.526797 1.1:-68332799 None:intergenic
CGCGGAGGAGGGCAATGAAG+CGG 0.533925 1.1:-68330534 None:intergenic
TCAAGGACAGAAATAGGACT+TGG 0.539012 1.1:-68332329 None:intergenic
CTTGCAGAGAAGAAAATGGC+TGG 0.540090 1.1:+68330431 None:intergenic
GTCCGCAAGGCACCGGAATG+GGG 0.540180 1.1:-68332397 None:intergenic
TTGCTGACAGCTGATGATGA+TGG 0.540546 1.1:-68332275 None:intergenic
CTGTCAGCAAGGAGGACTCT+GGG 0.542617 1.1:+68332288 MS.gene49445:CDS
AGCAGTAGTTCCTGCTCTGG+TGG 0.543729 1.1:+68332353 MS.gene49445:CDS
TTGTAGTTTGCCGCGGAGGA+GGG 0.553031 1.1:-68330545 None:intergenic
CAATAGTACTTCAGACCTCT+CGG 0.555740 1.1:+68330616 MS.gene49445:CDS
TTGGTGCGTGCACGTCCGCA+AGG 0.556777 1.1:-68332410 None:intergenic
TAGCAGCAGGCCGGTATTCG+GGG 0.557503 1.1:-68332808 None:intergenic
CATGTTCTCCTCAACAGAAG+AGG 0.558411 1.1:-68332492 None:intergenic
ACGTCCGCAAGGCACCGGAA+TGG 0.559647 1.1:-68332399 None:intergenic
AAAATCTGAAGAAAGCGCCT+TGG 0.566825 1.1:-68332534 None:intergenic
CGAGTTGTAGTTTGCCGCGG+AGG 0.567794 1.1:-68330549 None:intergenic
TCAGCTGGAGCAGTAGGAAG+TGG 0.567950 1.1:-68332641 None:intergenic
CCACTGTCAGCTGGAGCAGT+AGG 0.567950 1.1:-68332647 None:intergenic
GGAGAGTCTCACAATGAAAG+TGG 0.570456 1.1:+68332244 MS.gene49445:CDS
AACACCGAGTCAGACTCATT+CGG 0.575075 1.1:-68330587 None:intergenic
TCCTCAACAGAAGAGGAAGT+AGG 0.578279 1.1:-68332485 None:intergenic
CGCGGCAAACTACAACTCGT+TGG 0.579753 1.1:+68330553 MS.gene49445:CDS
AACCAAGACTCCACAATGGA+GGG 0.579995 1.1:+68332718 MS.gene49445:CDS
AAGACTCCACAATGGAGGGC+AGG 0.580027 1.1:+68332722 MS.gene49445:CDS
CAAAACGTTGGACTAGCAGC+AGG 0.583276 1.1:-68332821 None:intergenic
GTCCCCATTCCGGTGCCTTG+CGG 0.585255 1.1:+68332395 MS.gene49445:CDS
GCTGACAGTGGCGAACAGAA+TGG 0.590330 1.1:+68332659 MS.gene49445:CDS
TTGCGGTGTACGCTACAAGT+CGG 0.591752 1.1:+68332775 MS.gene49445:CDS
GTATTCCAAGGCGAGAACAT+TGG 0.594504 1.1:-68332936 None:intergenic
AAAACACTCTGCAATGCTTG+CGG 0.597816 1.1:+68332758 MS.gene49445:CDS
CTGGCCGAATGAGTCTGACT+CGG 0.600304 1.1:+68330583 MS.gene49445:CDS
ATCATCATCAGCTGTCAGCA+AGG 0.601103 1.1:+68332277 MS.gene49445:CDS
CGCTTCATTGCCCTCCTCCG+CGG 0.601691 1.1:+68330535 MS.gene49445:CDS
CAACGAGTTGTAGTTTGCCG+CGG 0.602863 1.1:-68330552 None:intergenic
TGCACAGCTGGACAAAACGT+TGG 0.609672 1.1:-68332833 None:intergenic
GGTCCTTGAAATGAGATGCA+AGG 0.611789 1.1:+68332874 MS.gene49445:CDS
ACGTTGGACTAGCAGCAGGC+CGG 0.619617 1.1:-68332817 None:intergenic
CTCAACAGAAGAGGAAGTAG+GGG 0.627104 1.1:-68332483 None:intergenic
TAACCAAGACTCCACAATGG+AGG 0.629519 1.1:+68332717 MS.gene49445:CDS
CGGTGTCAACGTCGTCGACG+GGG 0.629631 1.1:-68330508 None:intergenic
GCCTAATGTTATCTCCACCA+AGG 0.643801 1.1:+68332517 MS.gene49445:CDS
GCTCTGGTGGAAAGACCACA+GGG 0.652323 1.1:+68332366 MS.gene49445:CDS
ATGAGTTGCATAGCAGAACG+AGG 0.660947 1.1:-68332458 None:intergenic
AACGAGGGTTGAAGGCTGTG+GGG 0.663882 1.1:-68332442 None:intergenic
AATGGAGGGCAGGTCCAATG+GGG 0.678887 1.1:+68332732 MS.gene49445:CDS
CAACGGGGAGCTCCGCCGAG+AGG 0.679446 1.1:-68330631 None:intergenic
CCTACTGCTCCAGCTGACAG+TGG 0.685510 1.1:+68332647 MS.gene49445:CDS
ATCATCAGCTGTCAGCAAGG+AGG 0.691864 1.1:+68332280 MS.gene49445:CDS
GGAGTGGGTGTCCAATATTG+TGG 0.693703 1.1:+68332205 MS.gene49445:CDS
TGAGTTGCATAGCAGAACGA+GGG 0.714987 1.1:-68332457 None:intergenic
AGATAACCAAGACTCCACAA+TGG 0.734467 1.1:+68332714 MS.gene49445:CDS
CTCTACTCACCGGATCAACG+GGG 0.746525 1.1:-68330646 None:intergenic
AATGGGGACGTAAATCCCTG+TGG 0.749532 1.1:-68332381 None:intergenic
TAGTACTTCAGACCTCTCGG+CGG 0.759629 1.1:+68330619 MS.gene49445:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATAAAACAAACA+TGG - chr1.1:68331709-68331728 None:intergenic 10.0%
!! AAATAAAATAAAACAAACAT+GGG - chr1.1:68331708-68331727 None:intergenic 10.0%
!! TCTACAAAAAAAAAATAATA+TGG - chr1.1:68331104-68331123 None:intergenic 10.0%
!!! AACTTTACTTTATTTATTTT+TGG - chr1.1:68331340-68331359 None:intergenic 10.0%
!!! AATATAAAGTATTTATAGAA+AGG + chr1.1:68331466-68331485 MS.gene49445:intron 10.0%
!!! ATATAAAGTATTTATAGAAA+GGG + chr1.1:68331467-68331486 MS.gene49445:intron 10.0%
!! AAAAATTATTTCGTACTAAT+AGG + chr1.1:68330734-68330753 MS.gene49445:intron 15.0%
!! TAAAAATTAACCTAAAAAAG+TGG - chr1.1:68331243-68331262 None:intergenic 15.0%
!!! AAGAGTAATATACTTTTTTT+TGG + chr1.1:68331780-68331799 MS.gene49445:intron 15.0%
!!! AATAAAGTTTTTTTTTATCG+AGG + chr1.1:68331051-68331070 MS.gene49445:intron 15.0%
!!! TATAAGTTAGTAAAATCTTT+TGG - chr1.1:68331032-68331051 None:intergenic 15.0%
!! ATTATTTGGATCATCATTAT+TGG + chr1.1:68331585-68331604 MS.gene49445:intron 20.0%
!! TAAATAATGAACCTTCAAAT+TGG + chr1.1:68331660-68331679 MS.gene49445:intron 20.0%
!! TGAAGGTTCATTATTTATTT+AGG - chr1.1:68331657-68331676 None:intergenic 20.0%
!!! GTCGAAAAATGATTTTTTTA+AGG + chr1.1:68331152-68331171 MS.gene49445:intron 20.0%
!!! TAAAGGAATTTTCTACTTTT+TGG - chr1.1:68331393-68331412 None:intergenic 20.0%
!!! TGGTTTTTCTTTTTTGATAA+AGG + chr1.1:68330866-68330885 MS.gene49445:intron 20.0%
! AAAAACAAAACATGTGTGTA+TGG - chr1.1:68331922-68331941 None:intergenic 25.0%
! AATCACGATAAGTCAATAAA+AGG - chr1.1:68330944-68330963 None:intergenic 25.0%
! AGGTTCATTATTTATTTAGG+TGG - chr1.1:68331654-68331673 None:intergenic 25.0%
! ATTAAAATGTCATTCACATG+TGG - chr1.1:68331847-68331866 None:intergenic 25.0%
! CAAGAAGAAAAAGAAAATCA+AGG + chr1.1:68332616-68332635 MS.gene49445:CDS 25.0%
! GAAAATTCCTTTACTTGAAT+TGG + chr1.1:68331400-68331419 MS.gene49445:intron 25.0%
! GCAATTTATTGATCAAATTG+TGG + chr1.1:68331989-68332008 MS.gene49445:intron 25.0%
! GGTTCATTATTTATTTAGGT+GGG - chr1.1:68331653-68331672 None:intergenic 25.0%
! TATTTATAGAAAGGGTAGTT+TGG + chr1.1:68331475-68331494 MS.gene49445:intron 25.0%
! TCGAACTGTAATAATATAAC+AGG - chr1.1:68332170-68332189 None:intergenic 25.0%
! TCTTCATCTATTATTCTTAG+AGG - chr1.1:68332069-68332088 None:intergenic 25.0%
! TGATCAATAAATTGCATCAT+TGG - chr1.1:68331984-68332003 None:intergenic 25.0%
!! CTTGATTTTCTTTTTCTTCT+TGG - chr1.1:68332618-68332637 None:intergenic 25.0%
!!! AATCATTTTTCGACATGAAT+TGG - chr1.1:68331146-68331165 None:intergenic 25.0%
!!! CATGATACTTATTGATTCAA+TGG - chr1.1:68331947-68331966 None:intergenic 25.0%
AAATGAATCGTCCACAATAT+TGG - chr1.1:68332219-68332238 None:intergenic 30.0%
AAGAATAAGAACTTGTGCTT+TGG - chr1.1:68331749-68331768 None:intergenic 30.0%
ATAATGAACCTTCAAATTGG+TGG + chr1.1:68331663-68331682 MS.gene49445:intron 30.0%
ATGACTTCCAATTCAAGTAA+AGG - chr1.1:68331410-68331429 None:intergenic 30.0%
TTGGTTTCTTGACAATAATC+CGG - chr1.1:68332577-68332596 None:intergenic 30.0%
!!! ATTCTTAGAGGCTAGTTTTA+TGG - chr1.1:68332057-68332076 None:intergenic 30.0%
!!! TTATTTTTGGTCAAGTAGAG+TGG - chr1.1:68331327-68331346 None:intergenic 30.0%
!!! TTGCATATTGCCACTTTTTT+AGG + chr1.1:68331230-68331249 MS.gene49445:intron 30.0%
AAACCATTCATGATGTTGCT+AGG - chr1.1:68330973-68330992 None:intergenic 35.0%
AAAGCTACAAACAGACAATC+AGG - chr1.1:68330817-68330836 None:intergenic 35.0%
AACCATTCATGATGTTGCTA+GGG - chr1.1:68330972-68330991 None:intergenic 35.0%
AAGATCTTCTTTCATTGGAG+TGG + chr1.1:68332189-68332208 MS.gene49445:CDS 35.0%
AGATCTTCTTTCATTGGAGT+GGG + chr1.1:68332190-68332209 MS.gene49445:CDS 35.0%
AGTGGCAATATGCAATTGAT+TGG - chr1.1:68331225-68331244 None:intergenic 35.0%
CATCATAATAGTTCCGTGTA+CGG + chr1.1:68330790-68330809 MS.gene49445:intron 35.0%
GTCATAATCAAGCAATCAGA+CGG - chr1.1:68330771-68330790 None:intergenic 35.0%
GTTCGAAGATCTTCTTTCAT+TGG + chr1.1:68332184-68332203 MS.gene49445:intron 35.0%
TAGATTGACCACCAATTTGA+AGG - chr1.1:68331674-68331693 None:intergenic 35.0%
TATCCTTGCATCTCATTTCA+AGG - chr1.1:68332880-68332899 None:intergenic 35.0%
TGGTTTCTTGACAATAATCC+GGG - chr1.1:68332576-68332595 None:intergenic 35.0%
TTAAGTAACTCTCTACTCAC+CGG - chr1.1:68330659-68330678 None:intergenic 35.0%
TTCAGGAAACTTGATTGTGT+CGG + chr1.1:68331505-68331524 MS.gene49445:intron 35.0%
! AAACAAACATGGGTTGTTAC+TGG - chr1.1:68331698-68331717 None:intergenic 35.0%
! TTGAAAATTTTGCTGAGTCC+CGG + chr1.1:68332555-68332574 MS.gene49445:CDS 35.0%
!!! GGTAGTTTGGTCATTTTTTC+AGG + chr1.1:68331488-68331507 MS.gene49445:intron 35.0%
AAAACACTCTGCAATGCTTG+CGG + chr1.1:68332758-68332777 MS.gene49445:CDS 40.0%
AAGAAACCAAAGCTCTCTTC+TGG + chr1.1:68332587-68332606 MS.gene49445:CDS 40.0%
ACTATTTCCAGCTTGTCACA+CGG + chr1.1:68330837-68330856 MS.gene49445:intron 40.0%
AGAATGGTTCACTACCTGTT+AGG + chr1.1:68332675-68332694 MS.gene49445:CDS 40.0%
AGATAACCAAGACTCCACAA+TGG + chr1.1:68332714-68332733 MS.gene49445:CDS 40.0%
CAATAGTACTTCAGACCTCT+CGG + chr1.1:68330616-68330635 MS.gene49445:CDS 40.0%
CAATGCATGCATTTCCTAAC+AGG - chr1.1:68332692-68332711 None:intergenic 40.0%
CTGCTTTCAAGGACAGAAAT+AGG - chr1.1:68332338-68332357 None:intergenic 40.0%
TCAAGGACAGAAATAGGACT+TGG - chr1.1:68332332-68332351 None:intergenic 40.0%
TTCCCTAGCAACATCATGAA+TGG + chr1.1:68330967-68330986 MS.gene49445:intron 40.0%
! AAGGACCTTTTTGTGCGAAT+TGG - chr1.1:68332861-68332880 None:intergenic 40.0%
! TGTGGACGATTCATTTTGTG+AGG + chr1.1:68332223-68332242 MS.gene49445:CDS 40.0%
!! AAAATCTGAAGAAAGCGCCT+TGG - chr1.1:68332537-68332556 None:intergenic 40.0%
!! CAAGCATTGCAGAGTGTTTT+TGG - chr1.1:68332758-68332777 None:intergenic 40.0%
AAACACCAATGTTCTCGCCT+TGG + chr1.1:68332931-68332950 MS.gene49445:CDS 45.0%
AACACCGAGTCAGACTCATT+CGG - chr1.1:68330590-68330609 None:intergenic 45.0%
AACCAAGACTCCACAATGGA+GGG + chr1.1:68332718-68332737 MS.gene49445:CDS 45.0%
ACATAATCCGTGTGACAAGC+TGG - chr1.1:68330847-68330866 None:intergenic 45.0%
ACCCCTCAAATTGGAGAGTA+GGG - chr1.1:68331554-68331573 None:intergenic 45.0%
ACCCTACTCTCCAATTTGAG+GGG + chr1.1:68331550-68331569 MS.gene49445:intron 45.0%
AGCAGGAACTACTGCTTTCA+AGG - chr1.1:68332349-68332368 None:intergenic 45.0%
ATCATCATCAGCTGTCAGCA+AGG + chr1.1:68332277-68332296 MS.gene49445:CDS 45.0%
ATGAGTTGCATAGCAGAACG+AGG - chr1.1:68332461-68332480 None:intergenic 45.0%
CACCCTACTCTCCAATTTGA+GGG + chr1.1:68331549-68331568 MS.gene49445:intron 45.0%
CATGTTCTCCTCAACAGAAG+AGG - chr1.1:68332495-68332514 None:intergenic 45.0%
CCTCAACAGAAGAGGAAGTA+GGG - chr1.1:68332487-68332506 None:intergenic 45.0%
CTCAACAGAAGAGGAAGTAG+GGG - chr1.1:68332486-68332505 None:intergenic 45.0%
GCACTCCAATTCGCACAAAA+AGG + chr1.1:68332853-68332872 MS.gene49445:CDS 45.0%
GCCTAATGTTATCTCCACCA+AGG + chr1.1:68332517-68332536 MS.gene49445:CDS 45.0%
GCCTTGGTGGAGATAACATT+AGG - chr1.1:68332521-68332540 None:intergenic 45.0%
GGAGAGTCTCACAATGAAAG+TGG + chr1.1:68332244-68332263 MS.gene49445:CDS 45.0%
GGTCCTTGAAATGAGATGCA+AGG + chr1.1:68332874-68332893 MS.gene49445:CDS 45.0%
GGTGCGCTGTCTCAATTATT+TGG + chr1.1:68331571-68331590 MS.gene49445:intron 45.0%
GTATTCCAAGGCGAGAACAT+TGG - chr1.1:68332939-68332958 None:intergenic 45.0%
TAACCAAGACTCCACAATGG+AGG + chr1.1:68332717-68332736 MS.gene49445:CDS 45.0%
TCCTCAACAGAAGAGGAAGT+AGG - chr1.1:68332488-68332507 None:intergenic 45.0%
TGAGTTGCATAGCAGAACGA+GGG - chr1.1:68332460-68332479 None:intergenic 45.0%
! AAGGCGAGAACATTGGTGTT+TGG - chr1.1:68332932-68332951 None:intergenic 45.0%
! AGCTTGTCACACGGATTATG+TGG + chr1.1:68330846-68330865 MS.gene49445:intron 45.0%
! TTGATTGTGTCGGTGACATG+TGG + chr1.1:68331515-68331534 MS.gene49445:intron 45.0%
! TTGCTGACAGCTGATGATGA+TGG - chr1.1:68332278-68332297 None:intergenic 45.0%
!!! TTATTTTTATTTTCTAAAAT+TGG + chr1.1:68331366-68331385 MS.gene49445:intron 5.0%
AATGGGGACGTAAATCCCTG+TGG - chr1.1:68332384-68332403 None:intergenic 50.0%
ACAACTCGTTGGCGAGTATC+TGG + chr1.1:68330564-68330583 MS.gene49445:CDS 50.0%
AGGGATTTACGTCCCCATTC+CGG + chr1.1:68332385-68332404 MS.gene49445:CDS 50.0%
ATCATCAGCTGTCAGCAAGG+AGG + chr1.1:68332280-68332299 MS.gene49445:CDS 50.0%
CACCCCTCAAATTGGAGAGT+AGG - chr1.1:68331555-68331574 None:intergenic 50.0%
CAGACAATCAGGTCCGTACA+CGG - chr1.1:68330806-68330825 None:intergenic 50.0%
CATAGCAGAACGAGGGTTGA+AGG - chr1.1:68332453-68332472 None:intergenic 50.0%
CCACCCTACTCTCCAATTTG+AGG + chr1.1:68331548-68331567 MS.gene49445:intron 50.0%
CCCTACTTCCTCTTCTGTTG+AGG + chr1.1:68332484-68332503 MS.gene49445:CDS 50.0%
CCTCAAATTGGAGAGTAGGG+TGG - chr1.1:68331551-68331570 None:intergenic 50.0%
CTCTCTACTCACCGGATCAA+CGG - chr1.1:68330651-68330670 None:intergenic 50.0%
GAAAGCAGTAGTTCCTGCTC+TGG + chr1.1:68332350-68332369 MS.gene49445:CDS 50.0%
GGAGTGGGTGTCCAATATTG+TGG + chr1.1:68332205-68332224 MS.gene49445:CDS 50.0%
GTCTCTCCAGAAGAGAGCTT+TGG - chr1.1:68332596-68332615 None:intergenic 50.0%
TAGTACTTCAGACCTCTCGG+CGG + chr1.1:68330619-68330638 MS.gene49445:CDS 50.0%
TCTCTACTCACCGGATCAAC+GGG - chr1.1:68330650-68330669 None:intergenic 50.0%
TGCACAGCTGGACAAAACGT+TGG - chr1.1:68332836-68332855 None:intergenic 50.0%
TTGCGGTGTACGCTACAAGT+CGG + chr1.1:68332775-68332794 MS.gene49445:CDS 50.0%
! CAACGAGTTGTAGTTTGCCG+CGG - chr1.1:68330555-68330574 None:intergenic 50.0%
!! ATCTGAAGAAAGCGCCTTGG+TGG - chr1.1:68332534-68332553 None:intergenic 50.0%
!! CAAAACGTTGGACTAGCAGC+AGG - chr1.1:68332824-68332843 None:intergenic 50.0%
!!! CAGAGTGTTTTTGGCCCCAT+TGG - chr1.1:68332749-68332768 None:intergenic 50.0%
!!! GAGTCTGACTCGGTGTTTTC+TGG + chr1.1:68330593-68330612 MS.gene49445:CDS 50.0%
AACGAGGGTTGAAGGCTGTG+GGG - chr1.1:68332445-68332464 None:intergenic 55.0%
AAGACTCCACAATGGAGGGC+AGG + chr1.1:68332722-68332741 MS.gene49445:CDS 55.0%
AATGGAGGGCAGGTCCAATG+GGG + chr1.1:68332732-68332751 MS.gene49445:CDS 55.0%
ACAATGGAGGGCAGGTCCAA+TGG + chr1.1:68332730-68332749 MS.gene49445:CDS 55.0%
ACTAGCAGCAGGCCGGTATT+CGG - chr1.1:68332813-68332832 None:intergenic 55.0%
AGAACGAGGGTTGAAGGCTG+TGG - chr1.1:68332447-68332466 None:intergenic 55.0%
AGACAGCGCACCCCTCAAAT+TGG - chr1.1:68331563-68331582 None:intergenic 55.0%
AGCAGTAGTTCCTGCTCTGG+TGG + chr1.1:68332353-68332372 MS.gene49445:CDS 55.0%
ATTGGACCTGCCCTCCATTG+TGG - chr1.1:68332731-68332750 None:intergenic 55.0%
CAATGGAGGGCAGGTCCAAT+GGG + chr1.1:68332731-68332750 MS.gene49445:CDS 55.0%
CGCGGCAAACTACAACTCGT+TGG + chr1.1:68330553-68330572 MS.gene49445:CDS 55.0%
CTCTACTCACCGGATCAACG+GGG - chr1.1:68330649-68330668 None:intergenic 55.0%
CTGGCCGAATGAGTCTGACT+CGG + chr1.1:68330583-68330602 MS.gene49445:CDS 55.0%
CTGTCAGCAAGGAGGACTCT+GGG + chr1.1:68332288-68332307 MS.gene49445:CDS 55.0%
GAACGAGGGTTGAAGGCTGT+GGG - chr1.1:68332446-68332465 None:intergenic 55.0%
GCCGACTATTCCCCGAATAC+CGG + chr1.1:68332798-68332817 MS.gene49445:CDS 55.0%
GCCGGTATTCGGGGAATAGT+CGG - chr1.1:68332802-68332821 None:intergenic 55.0%
GCTCTGGTGGAAAGACCACA+GGG + chr1.1:68332366-68332385 MS.gene49445:CDS 55.0%
GCTGACAGTGGCGAACAGAA+TGG + chr1.1:68332659-68332678 MS.gene49445:CDS 55.0%
TCAGCTGGAGCAGTAGGAAG+TGG - chr1.1:68332644-68332663 None:intergenic 55.0%
TGCCCTCCATTGTGGAGTCT+TGG - chr1.1:68332723-68332742 None:intergenic 55.0%
TGCGAATTGGAGTGCACAGC+TGG - chr1.1:68332848-68332867 None:intergenic 55.0%
TGCGGTGTACGCTACAAGTC+GGG + chr1.1:68332776-68332795 MS.gene49445:CDS 55.0%
TGCTCTGGTGGAAAGACCAC+AGG + chr1.1:68332365-68332384 MS.gene49445:CDS 55.0%
TGTGGTCTTTCCACCAGAGC+AGG - chr1.1:68332366-68332385 None:intergenic 55.0%
TTCTGTTCGCCACTGTCAGC+TGG - chr1.1:68332659-68332678 None:intergenic 55.0%
! TTGTAGTTTGCCGCGGAGGA+GGG - chr1.1:68330548-68330567 None:intergenic 55.0%
CCACTGTCAGCTGGAGCAGT+AGG - chr1.1:68332650-68332669 None:intergenic 60.0%
CCTACTGCTCCAGCTGACAG+TGG + chr1.1:68332647-68332666 MS.gene49445:CDS 60.0%
CTAGCAGCAGGCCGGTATTC+GGG - chr1.1:68332812-68332831 None:intergenic 60.0%
GCTGTCAGCAAGGAGGACTC+TGG + chr1.1:68332287-68332306 MS.gene49445:CDS 60.0%
TAGCAGCAGGCCGGTATTCG+GGG - chr1.1:68332811-68332830 None:intergenic 60.0%
! AGCGGTGTCAACGTCGTCGA+CGG - chr1.1:68330513-68330532 None:intergenic 60.0%
! CGAGTTGTAGTTTGCCGCGG+AGG - chr1.1:68330552-68330571 None:intergenic 60.0%
! GTTGTAGTTTGCCGCGGAGG+AGG - chr1.1:68330549-68330568 None:intergenic 60.0%
!! ACGTTGGACTAGCAGCAGGC+CGG - chr1.1:68332820-68332839 None:intergenic 60.0%
CGCGGAGGAGGGCAATGAAG+CGG - chr1.1:68330537-68330556 None:intergenic 65.0%
CGCTTCATTGCCCTCCTCCG+CGG + chr1.1:68330535-68330554 MS.gene49445:CDS 65.0%
GGAGGGCAATGAAGCGGCAG+CGG - chr1.1:68330531-68330550 None:intergenic 65.0%
GTCCCCATTCCGGTGCCTTG+CGG + chr1.1:68332395-68332414 MS.gene49445:CDS 65.0%
TTGGTGCGTGCACGTCCGCA+AGG - chr1.1:68332413-68332432 None:intergenic 65.0%
! CGGTGTCAACGTCGTCGACG+GGG - chr1.1:68330511-68330530 None:intergenic 65.0%
! GCGGTGTCAACGTCGTCGAC+GGG - chr1.1:68330512-68330531 None:intergenic 65.0%
!! ACGTCCGCAAGGCACCGGAA+TGG - chr1.1:68332402-68332421 None:intergenic 65.0%
!! CGTCCGCAAGGCACCGGAAT+GGG - chr1.1:68332401-68332420 None:intergenic 65.0%
!! GTCCGCAAGGCACCGGAATG+GGG - chr1.1:68332400-68332419 None:intergenic 65.0%
CGTGCACGTCCGCAAGGCAC+CGG - chr1.1:68332407-68332426 None:intergenic 70.0%
GGCGGAGCTCCCCGTTGATC+CGG + chr1.1:68330637-68330656 MS.gene49445:CDS 70.0%
GGGTTGAAGGCTGTGGGGCG+TGG - chr1.1:68332440-68332459 None:intergenic 70.0%
CAACGGGGAGCTCCGCCGAG+AGG - chr1.1:68330634-68330653 None:intergenic 75.0%
GGCTGTGGGGCGTGGACGCT+TGG - chr1.1:68332432-68332451 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 68330446 68332967 68330446 ID=MS.gene49445
chr1.1 mRNA 68330446 68332967 68330446 ID=MS.gene49445.t1;Parent=MS.gene49445
chr1.1 exon 68330446 68330658 68330446 ID=MS.gene49445.t1.exon1;Parent=MS.gene49445.t1
chr1.1 CDS 68330446 68330658 68330446 ID=cds.MS.gene49445.t1;Parent=MS.gene49445.t1
chr1.1 exon 68332185 68332967 68332185 ID=MS.gene49445.t1.exon2;Parent=MS.gene49445.t1
chr1.1 CDS 68332185 68332967 68332185 ID=cds.MS.gene49445.t1;Parent=MS.gene49445.t1
Gene Sequence

>MS.gene49445

ATGGCTGGAACAATGAACGAGATTGATTGCTTCAACTTATTTGACAACATTGATGACATCTACCCCGTCGACGACGTTGACACCGCTGCCGCTTCATTGCCCTCCTCCGCGGCAAACTACAACTCGTTGGCGAGTATCTGGCCGAATGAGTCTGACTCGGTGTTTTCTGGCAATAGTACTTCAGACCTCTCGGCGGAGCTCCCCGTTGATCCGGTGAGTAGAGAGTTACTTAAAGTATAATTTATTTTATTTTGTTGTTATTATTTCATAATAACAGCTTTGTCAGAAAAAAATTATTTCGTACTAATAGGTTTGTTTGTAACCGTCTGATTGCTTGATTATGACATCATAATAGTTCCGTGTACGGACCTGATTGTCTGTTTGTAGCTTTACTATTTCCAGCTTGTCACACGGATTATGTGGTTTTTCTTTTTTGATAAAGGATTACGTGTTTTTTTCTAAATTCTTTTTTATTGACTCATTTTGATTTATATTCCTTTTATTGACTTATCGTGATTTTTTTCCCTAGCAACATCATGAATGGTTTACAGTTCAAAATTTAAAAAAAGAAAAGAAAAACAGTCCAAAAGATTTTACTAACTTATAATAAAGTTTTTTTTTATCGAGGAAATTCATTTTCATGTTTTTTTCTTTGCCATATTATTTTTTTTTTGTAGAGAATTTCAAAATTTTAAATCCAATTCATGTCGAAAAATGATTTTTTTAAGGTTTAATTTATATACATTTGACAATTTAAATTATTTGCACGTTTAAATCCAATCAATTGCATATTGCCACTTTTTTAGGTTAATTTTTAAAATATCTTCTTAAATATCTATTTAATATAATTTACTTATATACACTTATAAAAAATCAAACCACTCTACTTGACCAAAAATAAATAAAGTAAAGTTAATATTTTATTTTTATTTTCTAAAATTGGTCCAAAAAGTAGAAAATTCCTTTACTTGAATTGGAAGTCATTATTATTAACTAATTAAGAACTTTTATAATACTATAAATATAAAGTATTTATAGAAAGGGTAGTTTGGTCATTTTTTCAGGAAACTTGATTGTGTCGGTGACATGTGGCACATCACTTCCACCCTACTCTCCAATTTGAGGGGTGCGCTGTCTCAATTATTTGGATCATCATTATTGGTTGTCATGTGCTTTTGCATGTGATTATCACGTGATGTGAGCACCCACCTAAATAAATAATGAACCTTCAAATTGGTGGTCAATCTAGCCAGTAACAACCCATGTTTGTTTTATTTTATTTTATTTTATTTAATTCATACCAAAGCACAAGTTCTTATTCTTCATTGAATTTGAAGAGTAATATACTTTTTTTTGGTTGTATATGCTCTACTAAACTGCATTCTTTGCTTGAACTGTCCACATGTGAATGACATTTTAATAAGTGATTGTGTTTTTACTTCAATATTAGTTATATGTAAAAACATGCTTGCACCATACACACATGTTTTGTTTTTCACCATTGAATCAATAAGTATCATGATATCTCATTTGATCCAATGATGCAATTTATTGATCAAATTGTGGAAAATAACTTGTGCGTGATGCAAAACTTATCTCATTTACGCACCATAAAACTAGCCTCTAAGAATAATAGATGAAGATGTGAATCTAAGTTTTATTTTTATTACAATCTAAGTTTAAGTTATGTTTCTTCTAATAAAATTGTGATTTTGATCAATCCTGTTATATTATTACAGTTCGAAGATCTTCTTTCATTGGAGTGGGTGTCCAATATTGTGGACGATTCATTTTGTGAGGAGAGTCTCACAATGAAAGTGGAGCAGCAGCCATCATCATCAGCTGTCAGCAAGGAGGACTCTGGGCATTATCAATTTCAGACACCAAGTCCTATTTCTGTCCTTGAAAGCAGTAGTTCCTGCTCTGGTGGAAAGACCACAGGGATTTACGTCCCCATTCCGGTGCCTTGCGGACGTGCACGCACCAAGCGTCCACGCCCCACAGCCTTCAACCCTCGTTCTGCTATGCAACTCATTTCCCCTACTTCCTCTTCTGTTGAGGAGAACATGCAGCCTAATGTTATCTCCACCAAGGCGCTTTCTTCAGATTTTGAAAATTTTGCTGAGTCCCGGATTATTGTCAAGAAACCAAAGCTCTCTTCTGGAGAGACCAAGAAGAAAAAGAAAATCAAGGCACCACTTCCTACTGCTCCAGCTGACAGTGGCGAACAGAATGGTTCACTACCTGTTAGGAAATGCATGCATTGTGAGATAACCAAGACTCCACAATGGAGGGCAGGTCCAATGGGGCCAAAAACACTCTGCAATGCTTGCGGTGTACGCTACAAGTCGGGCCGACTATTCCCCGAATACCGGCCTGCTGCTAGTCCAACGTTTTGTCCAGCTGTGCACTCCAATTCGCACAAAAAGGTCCTTGAAATGAGATGCAAGGATAGTTTTGAATCAGATTCAGCTGAACTCATTCCAAACACCAATGTTCTCGCCTTGGAATACAACATTTGA
Protein sequence

>MS.gene49445.t1

MAGTMNEIDCFNLFDNIDDIYPVDDVDTAAASLPSSAANYNSLASIWPNESDSVFSGNSTSDLSAELPVDPFEDLLSLEWVSNIVDDSFCEESLTMKVEQQPSSSAVSKEDSGHYQFQTPSPISVLESSSSCSGGKTTGIYVPIPVPCGRARTKRPRPTAFNPRSAMQLISPTSSSVEENMQPNVISTKALSSDFENFAESRIIVKKPKLSSGETKKKKKIKAPLPTAPADSGEQNGSLPVRKCMHCEITKTPQWRAGPMGPKTLCNACGVRYKSGRLFPEYRPAASPTFCPAVHSNSHKKVLEMRCKDSFESDSAELIPNTNVLALEYNI