AlfalfaGEDB | MS.gene49607

M. sativa cultivar XinJiangDaYe / MS.gene49607

KEGG: K03671
GO: GO:0003674 GO:0003824 GO:0006662 GO:0008150 GO:0008152 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0018904 GO:0019725 GO:0042592 GO:0044281 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49607.t1	ACZ37071.1	100	127	0	0	1	127	1	127	3.90E-62	247.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49607.t1	Q38879	52.3	111	53	0	16	126	22	132	3.9e-31	135.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49607.t1	D8L5E2	100.0	127	0	0	1	127	1	127	2.8e-62	247.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057606	MS.gene49607	0.997638	3.08E-246	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49607.t1	MTR_5g037950	99.213	127	1	0	1	127	1	127	9.54e-91	258
MS.gene49607.t1	MTR_5g037930	72.441	127	34	1	1	126	1	127	1.45e-54	167
MS.gene49607.t1	MTR_5g037890	66.667	126	41	1	1	126	1	125	6.50e-53	162
MS.gene49607.t1	MTR_7g009070	60.577	104	41	0	23	126	30	133	7.25e-45	142
MS.gene49607.t1	MTR_3g112410	49.550	111	56	0	15	125	2	112	4.79e-38	125
MS.gene49607.t1	MTR_1g023140	43.750	112	63	0	15	126	2	113	1.37e-34	116
MS.gene49607.t1	MTR_5g021180	44.144	111	62	0	15	125	4	114	6.89e-34	114
MS.gene49607.t1	MTR_8g107460	43.137	102	58	0	21	122	256	357	2.50e-27	103
MS.gene49607.t1	MTR_4g081380	44.340	106	57	2	19	123	10	114	7.97e-26	94.4
MS.gene49607.t1	MTR_2g010750	40.952	105	62	0	19	123	9	113	2.39e-25	93.2
MS.gene49607.t1	MTR_2g082590	39.604	101	59	1	23	123	29	127	2.24e-23	88.6
MS.gene49607.t1	MTR_2g082590	35.238	105	68	0	19	123	10	114	3.36e-23	87.4
MS.gene49607.t1	MTR_8g005320	38.710	93	57	0	30	122	37	129	4.66e-23	87.8
MS.gene49607.t1	MTR_7g073680	39.560	91	55	0	30	120	35	125	4.82e-23	87.8
MS.gene49607.t1	MTR_8g006685	38.710	93	57	0	30	122	106	198	1.95e-22	87.8
MS.gene49607.t1	MTR_8g006560	38.710	93	57	0	30	122	106	198	1.95e-22	87.8
MS.gene49607.t1	MTR_1g098660	38.372	86	50	2	40	124	77	160	2.58e-19	79.0
MS.gene49607.t1	MTR_4g094690	39.024	82	48	1	45	124	85	166	9.95e-19	77.4
MS.gene49607.t1	MTR_7g080250	41.379	87	50	1	41	126	94	180	5.42e-16	70.5
MS.gene49607.t1	MTR_3g089065	37.349	83	50	2	44	124	89	171	4.50e-15	68.2
MS.gene49607.t1	MTR_2g079420	38.554	83	49	2	44	124	89	171	4.01e-14	65.5
MS.gene49607.t1	MTR_8g059015	36.145	83	51	2	44	124	92	174	2.04e-13	63.5
MS.gene49607.t1	MTR_1g114290	34.615	78	47	2	40	116	85	159	7.07e-13	62.4
MS.gene49607.t1	MTR_4g088905	32.500	80	54	0	45	124	25	104	1.22e-11	60.8
MS.gene49607.t1	MTR_4g081380	36.905	84	51	2	13	95	4	86	1.38e-11	57.0
MS.gene49607.t1	MTR_4g085880	31.579	76	51	1	44	118	84	159	6.77e-11	57.0
MS.gene49607.t1	MTR_2g079340	36.620	71	44	1	43	112	54	124	8.72e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49607.t1	AT5G39950	52.252	111	53	0	16	126	22	132	1.79e-44	142
MS.gene49607.t1	AT1G59730	43.077	130	70	2	1	127	1	129	1.16e-37	124
MS.gene49607.t1	AT3G51030	44.144	111	62	0	15	125	2	112	6.05e-34	114
MS.gene49607.t1	AT5G42980	48.113	106	55	0	21	126	7	112	3.14e-33	112
MS.gene49607.t1	AT1G69880	48.077	104	54	0	21	124	39	142	2.72e-32	111
MS.gene49607.t1	AT1G19730	45.536	112	60	1	15	125	2	113	4.15e-31	107
MS.gene49607.t1	AT1G45145	41.905	105	61	0	21	125	7	111	2.03e-30	105
MS.gene49607.t1	AT3G17880	39.474	114	65	1	13	122	253	366	1.54e-25	99.0
MS.gene49607.t1	AT3G17880	39.474	114	65	1	13	122	260	373	1.61e-25	99.4
MS.gene49607.t1	AT1G11530	39.806	103	62	0	21	123	4	106	7.44e-24	89.4
MS.gene49607.t1	AT3G08710	41.758	91	53	0	30	120	34	124	2.45e-23	88.6
MS.gene49607.t1	AT3G08710	41.758	91	53	0	30	120	34	124	2.45e-23	88.6
MS.gene49607.t1	AT3G08710	41.758	91	53	0	30	120	34	124	2.45e-23	88.6
MS.gene49607.t1	AT3G56420	38.043	92	57	0	29	120	50	141	4.44e-23	88.2
MS.gene49607.t1	AT3G56420	38.043	92	57	0	29	120	50	141	4.44e-23	88.2
MS.gene49607.t1	AT3G56420	38.043	92	57	0	29	120	50	141	4.44e-23	88.2
MS.gene49607.t1	AT2G40790	39.130	92	56	0	29	120	50	141	1.72e-19	79.0
MS.gene49607.t1	AT2G40790	39.130	92	56	0	29	120	50	141	1.72e-19	79.0
MS.gene49607.t1	AT1G43560	41.379	87	49	2	40	124	75	161	4.76e-19	78.2
MS.gene49607.t1	AT1G76760	38.372	86	51	2	40	123	80	165	2.28e-18	76.6
MS.gene49607.t1	AT5G16400	42.529	87	49	1	41	126	97	183	1.71e-17	74.7
MS.gene49607.t1	AT2G35010	40.244	82	47	1	45	124	110	191	6.68e-17	73.2
MS.gene49607.t1	AT2G35010	40.244	82	47	1	45	124	110	191	6.68e-17	73.2
MS.gene49607.t1	AT3G02730	43.023	86	48	1	42	126	88	173	8.91e-17	72.4
MS.gene49607.t1	AT3G15360	32.673	101	63	3	26	124	92	189	6.05e-15	68.2
MS.gene49607.t1	AT1G31020	39.024	82	48	1	45	124	75	156	7.98e-15	67.0
MS.gene49607.t1	AT1G03680	38.272	81	48	2	46	124	97	177	1.43e-14	67.0
MS.gene49607.t1	AT4G03520	33.735	83	53	2	44	124	101	183	1.13e-13	64.7
MS.gene49607.t1	AT1G52990	34.066	91	58	2	35	124	222	311	1.73e-13	65.9
MS.gene49607.t1	AT1G50320	39.189	74	41	2	44	116	90	160	3.80e-13	63.2
MS.gene49607.t1	AT4G04950	29.787	94	66	0	31	124	11	104	9.42e-12	61.2
MS.gene49607.t1	AT4G32580	30.864	81	56	0	44	124	24	104	9.86e-12	58.9
MS.gene49607.t1	AT1G07700	36.364	99	55	2	34	124	100	198	1.72e-11	59.3
MS.gene49607.t1	AT1G07700	36.364	99	55	2	34	124	100	198	1.72e-11	59.3
MS.gene49607.t1	AT1G07700	36.364	99	55	2	34	124	100	198	1.72e-11	59.3
MS.gene49607.t1	AT1G07700	36.364	99	55	2	34	124	113	211	3.61e-11	58.5

Find 25 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAGCTAATTCTTTGATAAT+TGG	0.122091	5.1:+28616986	None:intergenic
GGAGGTGGCAAGTGCATTTC+AGG	0.225705	5.1:-28616817	MS.gene49607:CDS
CTCAACAACAGACTCTAAAT+TGG	0.230357	5.1:+28617682	None:intergenic
GATTGACGTGGATGAGTTAA+TGG	0.268688	5.1:-28616936	MS.gene49607:intron
CCTTCCACTCAACTGCTAAA+TGG	0.287365	5.1:-28617631	MS.gene49607:CDS
CAGCTAATTCTTTGATAATT+GGG	0.312442	5.1:+28616987	None:intergenic
TACCAGCTTGTTTGTTACTT+TGG	0.383274	5.1:+28617589	None:intergenic
AGACTCTAAATTGGAAAAGT+TGG	0.412160	5.1:+28617691	None:intergenic
AAAGATGGTCATTGATTTCA+CGG	0.422756	5.1:-28617041	MS.gene49607:intron
TAAATGGAAGGCTCACTTTG+AGG	0.488391	5.1:-28617615	MS.gene49607:CDS
TCATTGATTTCACGGCTACT+TGG	0.488401	5.1:-28617033	MS.gene49607:CDS
CCATTTAGCAGTTGAGTGGA+AGG	0.494217	5.1:+28617631	None:intergenic
TTCTTCAATAGTATAAATGT+TGG	0.495545	5.1:+28616783	None:intergenic
GATTTCACGGCTACTTGGTG+TGG	0.515795	5.1:-28617028	MS.gene49607:CDS
ACATTTATACTATTGAAGAA+AGG	0.532669	5.1:-28616780	MS.gene49607:CDS
GTGTGGACCTTGCAAATACA+TGG	0.544731	5.1:-28617011	MS.gene49607:CDS
TTCCAAAGTAACAAACAAGC+TGG	0.568571	5.1:-28617591	MS.gene49607:intron
GAAACTGATTGAGAAACGTA+GGG	0.577131	5.1:-28616712	MS.gene49607:CDS
CCTTCCATTTAGCAGTTGAG+TGG	0.582577	5.1:+28617627	None:intergenic
AGAAACTGATTGAGAAACGT+AGG	0.583938	5.1:-28616713	MS.gene49607:CDS
ATTGGGTCCATGTATTTGCA+AGG	0.586486	5.1:+28617004	None:intergenic
CCACTCAACTGCTAAATGGA+AGG	0.588835	5.1:-28617627	MS.gene49607:CDS
TAGCAGTTGAGTGGAAGGTG+AGG	0.659322	5.1:+28617636	None:intergenic
TGAGTTCATTAAGATTGACG+TGG	0.684888	5.1:-28616948	MS.gene49607:CDS
TGGAAGGTGAGGATGAGCGA+TGG	0.701264	5.1:+28617647	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTTAATTATTTTTAAAGA+TGG	-	chr5.1:28617349-28617368	MS.gene49607:intron	10.0%
!!	TAATCAAATATATGTCATAT+AGG	+	chr5.1:28617212-28617231	None:intergenic	15.0%
!!	TATAGATATAAGATATCAAA+AGG	+	chr5.1:28617526-28617545	None:intergenic	15.0%
!!!	AGTTTTCATTTTTTTTTTGT+TGG	+	chr5.1:28617035-28617054	None:intergenic	15.0%
!!!	GCTAAAATTTTCTTATTATT+TGG	-	chr5.1:28617289-28617308	MS.gene49607:intron	15.0%
!!!	GTTAAATAATTTTTCTTAGA+GGG	-	chr5.1:28616953-28616972	MS.gene49607:CDS	15.0%
!!!	TGTTAAATAATTTTTCTTAG+AGG	-	chr5.1:28616952-28616971	MS.gene49607:CDS	15.0%
!!!	TTGTTGGTAAATTAATTATA+TGG	+	chr5.1:28617019-28617038	None:intergenic	15.0%
!!	ACATTTATACTATTGAAGAA+AGG	-	chr5.1:28617625-28617644	MS.gene49607:CDS	20.0%
!!	GATATCAAAAGGTAATTAAA+AGG	+	chr5.1:28617515-28617534	None:intergenic	20.0%
!!	TTCTTCAATAGTATAAATGT+TGG	+	chr5.1:28617625-28617644	None:intergenic	20.0%
!!!	TAATAAGAAAATTTTAGCCA+AGG	+	chr5.1:28617287-28617306	None:intergenic	20.0%
!!!	TTAAAATCGTTTGTTTTATG+AGG	-	chr5.1:28617313-28617332	MS.gene49607:intron	20.0%
!!!	TTTTTAGATACTTTGAAGTT+TGG	+	chr5.1:28616992-28617011	None:intergenic	20.0%
!	AAAACCAAGGTTTCAAAAAA+CGG	-	chr5.1:28617116-28617135	MS.gene49607:intron	25.0%
!	ATGACAACAATAACAATCAT+GGG	+	chr5.1:28617142-28617161	None:intergenic	25.0%
!!	AAATTTTGTGACGAGAAAAA+CGG	+	chr5.1:28617062-28617081	None:intergenic	25.0%
!!	CAGCTAATTCTTTGATAATT+GGG	+	chr5.1:28617421-28617440	None:intergenic	25.0%
!!!	TTTTTCATCGGTTAAAATCT+TGG	+	chr5.1:28617099-28617118	None:intergenic	25.0%
	AAAAGTAGTTGAAAAAGTGG+TGG	-	chr5.1:28617648-28617667	MS.gene49607:CDS	30.0%
	AAAGTAGTTGAAAAAGTGGT+GGG	-	chr5.1:28617649-28617668	MS.gene49607:CDS	30.0%
	AGACTCTAAATTGGAAAAGT+TGG	+	chr5.1:28616717-28616736	None:intergenic	30.0%
	AGGAAAAGTAGTTGAAAAAG+TGG	-	chr5.1:28617645-28617664	MS.gene49607:CDS	30.0%
	ATTAGATCAACACAACGATT+TGG	-	chr5.1:28616856-28616875	MS.gene49607:intron	30.0%
	CATGACAACAATAACAATCA+TGG	+	chr5.1:28617143-28617162	None:intergenic	30.0%
	GATAATGCAAAATGTACCAA+TGG	-	chr5.1:28617560-28617579	MS.gene49607:intron	30.0%
	TAACATTGAATGATTCGATG+AGG	+	chr5.1:28616895-28616914	None:intergenic	30.0%
	TTTAACCGATGAAAAAACCA+AGG	-	chr5.1:28617103-28617122	MS.gene49607:intron	30.0%
!	CTTAACATTTTCTTTGCACT+CGG	-	chr5.1:28617174-28617193	MS.gene49607:intron	30.0%
!	GCAGCTAATTCTTTGATAAT+TGG	+	chr5.1:28617422-28617441	None:intergenic	30.0%
!!	AAAGATGGTCATTGATTTCA+CGG	-	chr5.1:28617364-28617383	MS.gene49607:intron	30.0%
!!!	TGAAACCTTGGTTTTTTCAT+CGG	+	chr5.1:28617111-28617130	None:intergenic	30.0%
!!!	TTAGCCAAGGTTTTAAATCA+CGG	+	chr5.1:28617274-28617293	None:intergenic	30.0%
	AGAAACTGATTGAGAAACGT+AGG	-	chr5.1:28617692-28617711	MS.gene49607:CDS	35.0%
	CAAAATGTACCAATGGAAAC+AGG	-	chr5.1:28617567-28617586	MS.gene49607:intron	35.0%
	CTCAACAACAGACTCTAAAT+TGG	+	chr5.1:28616726-28616745	None:intergenic	35.0%
	GAAACTGATTGAGAAACGTA+GGG	-	chr5.1:28617693-28617712	MS.gene49607:CDS	35.0%
	TGAGTTCATTAAGATTGACG+TGG	-	chr5.1:28617457-28617476	MS.gene49607:intron	35.0%
	TTCCAAAGTAACAAACAAGC+TGG	-	chr5.1:28616814-28616833	MS.gene49607:CDS	35.0%
!	TACCAGCTTGTTTGTTACTT+TGG	+	chr5.1:28616819-28616838	None:intergenic	35.0%
	AATGTACCAATGGAAACAGG+AGG	-	chr5.1:28617570-28617589	MS.gene49607:intron	40.0%
	ATTGGGTCCATGTATTTGCA+AGG	+	chr5.1:28617404-28617423	None:intergenic	40.0%
	GATTGACGTGGATGAGTTAA+TGG	-	chr5.1:28617469-28617488	MS.gene49607:intron	40.0%
	TAAATGGAAGGCTCACTTTG+AGG	-	chr5.1:28616790-28616809	MS.gene49607:CDS	40.0%
!	AAAAGTGGTGGGAGCAAAAA+AGG	-	chr5.1:28617660-28617679	MS.gene49607:CDS	40.0%
!	ACGACCGTGATTTAAAACCT+TGG	-	chr5.1:28617267-28617286	MS.gene49607:intron	40.0%
!!	TCATTGATTTCACGGCTACT+TGG	-	chr5.1:28617372-28617391	MS.gene49607:intron	40.0%
!!!	AAGGTTTTAAATCACGGTCG+TGG	+	chr5.1:28617268-28617287	None:intergenic	40.0%
	CCACTCAACTGCTAAATGGA+AGG	-	chr5.1:28616778-28616797	MS.gene49607:CDS	45.0%
	CCATTTAGCAGTTGAGTGGA+AGG	+	chr5.1:28616777-28616796	None:intergenic	45.0%
	CCTTCCACTCAACTGCTAAA+TGG	-	chr5.1:28616774-28616793	MS.gene49607:CDS	45.0%
	CCTTCCATTTAGCAGTTGAG+TGG	+	chr5.1:28616781-28616800	None:intergenic	45.0%
	GTGTGGACCTTGCAAATACA+TGG	-	chr5.1:28617394-28617413	MS.gene49607:intron	45.0%
!!!	TGGGCCGTTTTTTGAAACCT+TGG	+	chr5.1:28617123-28617142	None:intergenic	45.0%
	GTACCAATGGAAACAGGAGG+TGG	-	chr5.1:28617573-28617592	MS.gene49607:intron	50.0%
	TAGCAGTTGAGTGGAAGGTG+AGG	+	chr5.1:28616772-28616791	None:intergenic	50.0%
	TTGCCACCTCCTGTTTCCAT+TGG	+	chr5.1:28617579-28617598	None:intergenic	50.0%
!	GATTTCACGGCTACTTGGTG+TGG	-	chr5.1:28617377-28617396	MS.gene49607:intron	50.0%
	GGAGGTGGCAAGTGCATTTC+AGG	-	chr5.1:28617588-28617607	MS.gene49607:intron	55.0%
	TGGAAGGTGAGGATGAGCGA+TGG	+	chr5.1:28616761-28616780	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	28616707	28617720	28616707	ID=MS.gene49607
chr5.1	mRNA	28616707	28617720	28616707	ID=MS.gene49607.t1;Parent=MS.gene49607
chr5.1	exon	28617592	28617720	28617592	ID=MS.gene49607.t1.exon1;Parent=MS.gene49607.t1
chr5.1	CDS	28617592	28617720	28617592	ID=cds.MS.gene49607.t1;Parent=MS.gene49607.t1
chr5.1	exon	28616937	28617059	28616937	ID=MS.gene49607.t1.exon2;Parent=MS.gene49607.t1
chr5.1	CDS	28616937	28617059	28616937	ID=cds.MS.gene49607.t1;Parent=MS.gene49607.t1
chr5.1	exon	28616707	28616838	28616707	ID=MS.gene49607.t1.exon3;Parent=MS.gene49607.t1
chr5.1	CDS	28616707	28616838	28616707	ID=cds.MS.gene49607.t1;Parent=MS.gene49607.t1

Gene Sequence

>MS.gene49607

ATGGGAGCCAACTTTTCCAATTTAGAGTCTGTTGTTGAGAAATCATCACAGCCATCGCTCATCCTCACCTTCCACTCAACTGCTAAATGGAAGGCTCACTTTGAGGCTTCCAAAGTAACAAACAAGCTGGTATAAACTTCTGATATATAATTAGATCAACACAACGATTTGGTTGAATATATACTCCTCATCGAATCATTCAATGTTATCTTATATTTTCATATTAATTTATTTTAAACTTCAAATGTTAAATAATTTTTCTTAGAGGGTATCAGTATTCAACCAAACTTCAAAGTATCTAAAAATTTACCATATAATTAATTTACCAACAAAAAAAAAATGAAAACTTTCTCCGTTTTTCTCGTCACAAAATTTAGTGACATGCACAACCAAGATTTTAACCGATGAAAAAACCAAGGTTTCAAAAAACGGCCCATGATTGTTATTGTTGTCATGAAGTCAAGTTTCTTAACATTTTCTTTGCACTCGGTTTGTCATTGCGCCTATATGACATATATTTGATTACAATTTGTTGCAATATCAAAATTCACGATACAACCACGACCGTGATTTAAAACCTTGGCTAAAATTTTCTTATTATTTGGCTTAAAATCGTTTGTTTTATGAGGTGATTAATTTTGTTTCTTAATTATTTTTAAAGATGGTCATTGATTTCACGGCTACTTGGTGTGGACCTTGCAAATACATGGACCCAATTATCAAAGAATTAGCTGCAAAATACAAAGATGTTGAGTTCATTAAGATTGACGTGGATGAGTTAATGGTATGATACATATGATTAATTCCTTTTAATTACCTTTTGATATCTTATATCTATATATATAAACAATATGATAATGCAAAATGTACCAATGGAAACAGGAGGTGGCAAGTGCATTTCAGGTGCAAGCAATGCCAACATTTATACTATTGAAGAAAGGAAAAGTAGTTGAAAAAGTGGTGGGAGCAAAAAAGGAGCAATTACAGAAACTGATTGAGAAACGTAGGGTTTGA

Protein sequence

>MS.gene49607.t1

MGANFSNLESVVEKSSQPSLILTFHSTAKWKAHFEASKVTNKLMVIDFTATWCGPCKYMDPIIKELAAKYKDVEFIKIDVDELMEVASAFQVQAMPTFILLKKGKVVEKVVGAKKEQLQKLIEKRRV