AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50562

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50562.t1 MTR_1g071100 96.429 112 1 1 1 112 1 109 1.25e-74 216
MS.gene50562.t1 MTR_1g076640 61.628 86 33 0 27 112 23 108 1.40e-32 110
MS.gene50562.t1 MTR_1g076580 55.385 65 29 0 20 84 16 80 3.57e-16 68.2
MS.gene50562.t1 MTR_1g076580 55.385 65 29 0 20 84 16 80 3.58e-16 67.8
MS.gene50562.t1 MTR_1g010050 37.037 81 49 1 33 111 45 125 6.28e-16 68.6
MS.gene50562.t1 MTR_1g076640 56.897 58 25 0 27 84 23 80 1.59e-14 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50562.t1 AT3G63420 52.874 87 41 0 26 112 12 98 1.02e-31 107
MS.gene50562.t1 AT3G63420 52.874 87 41 0 26 112 12 98 1.02e-31 107
MS.gene50562.t1 AT3G63420 50.000 100 46 1 13 112 43 138 3.22e-31 107
MS.gene50562.t1 AT3G22942 57.609 92 39 0 21 112 9 100 3.30e-31 106

Find 24 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGACCTGTAAACCTCTTA+TGG 0.220609 1.2:-54434584 MS.gene50562:CDS
TTCTCTGATAACAGAGTTCA+TGG 0.283632 1.2:-54434603 MS.gene50562:intron
ACAAGATACTAAATTTCTTC+AGG 0.319990 1.2:-54437307 MS.gene50562:intron
CTATCCCATAAGAGGTTTAC+AGG 0.323497 1.2:+54434579 None:intergenic
TAAACCTCTTATGGGATAGA+TGG 0.349715 1.2:-54434575 MS.gene50562:CDS
TGAATGTATTCAGGAAGAAT+TGG 0.367884 1.2:-54435437 MS.gene50562:intron
CAAACCATCTATCCCATAAG+AGG 0.395789 1.2:+54434571 None:intergenic
AGAAATTACTCTGGGATTAG+TGG 0.405087 1.2:+54434688 None:intergenic
GTGTGTTATGGCGTCTGAAA+CGG 0.409047 1.2:-54437448 None:intergenic
TACTCTGGGATTAGTGGATC+AGG 0.437858 1.2:+54434694 None:intergenic
TGGACCTGTAAACCTCTTAT+GGG 0.444843 1.2:-54434583 MS.gene50562:CDS
TTATGGGATAGATGGTTTGA+AGG 0.464564 1.2:-54434567 MS.gene50562:CDS
TAGATGGTTTGAAGGGCCTC+AGG 0.521777 1.2:-54434559 MS.gene50562:CDS
GTCTGAAACGGCGTCGTCTG+CGG 0.528907 1.2:-54437436 MS.gene50562:CDS
ATCCGCAAGCATGTAGATGT+TGG 0.541129 1.2:-54434536 MS.gene50562:CDS
TATGGGATAGATGGTTTGAA+GGG 0.555121 1.2:-54434566 MS.gene50562:CDS
GATACTGCGACTGATAAAAG+AGG 0.564553 1.2:-54437366 MS.gene50562:CDS
CAGCTGAAACAGAAACAACA+AGG 0.578916 1.2:+54437397 None:intergenic
GCGCAGATTGCTACAAAACA+TGG 0.600608 1.2:-54434725 MS.gene50562:intron
ATCCAACATCTACATGCTTG+CGG 0.601467 1.2:+54434534 None:intergenic
CTACATGCTTGCGGATCCTG+AGG 0.605370 1.2:+54434543 None:intergenic
AAATGTGTCGGCTATATGCA+AGG 0.619090 1.2:-54435395 MS.gene50562:CDS
CGAGAAAACAGAAAATGTGT+CGG 0.640254 1.2:-54435407 MS.gene50562:CDS
AGCTGAAACAGAAACAACAA+GGG 0.720366 1.2:+54437398 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAGAATTTTATTTTTATC+AGG + chr1.2:54435019-54435038 None:intergenic 10.0%
!! AAAAAACACATAAATACTAT+GGG + chr1.2:54435538-54435557 None:intergenic 15.0%
!! AAAATAAACTTAACTTATAG+CGG - chr1.2:54436224-54436243 MS.gene50562:intron 15.0%
!! AAATAAAAATAGATACTGTT+CGG + chr1.2:54436781-54436800 None:intergenic 15.0%
!! ATCAAAAAAGAAAAAATGTA+TGG + chr1.2:54436318-54436337 None:intergenic 15.0%
!!! AATTTTAAAAAATGAAGGTA+CGG + chr1.2:54436989-54437008 None:intergenic 15.0%
!!! TTTTTTTTTATAAAAACGAC+CGG - chr1.2:54435095-54435114 MS.gene50562:intron 15.0%
!! ACTAAAGATCATAAATTCAA+AGG + chr1.2:54435593-54435612 None:intergenic 20.0%
!! CAAAAAACACATAAATACTA+TGG + chr1.2:54435539-54435558 None:intergenic 20.0%
!! GATAAATTACAAAACAATGT+GGG - chr1.2:54435649-54435668 MS.gene50562:intron 20.0%
!! GTGTATAATGTATAACTAAA+GGG - chr1.2:54434725-54434744 MS.gene50562:intron 20.0%
!! TAATTGCTTAAATCAATGTT+AGG - chr1.2:54436389-54436408 MS.gene50562:intron 20.0%
!! TACAAAAAAAAAACCCAAAT+TGG + chr1.2:54435225-54435244 None:intergenic 20.0%
!! TGATAAATTACAAAACAATG+TGG - chr1.2:54435648-54435667 MS.gene50562:intron 20.0%
!! TGTGTATAATGTATAACTAA+AGG - chr1.2:54434724-54434743 MS.gene50562:intron 20.0%
!! TTTATAGCTTAAGCTTATAT+TGG - chr1.2:54435756-54435775 MS.gene50562:intron 20.0%
!!! ACTTGAGAGTTTTATTTTTT+TGG + chr1.2:54436900-54436919 None:intergenic 20.0%
!!! ATTTTAAAAAATGAAGGTAC+GGG + chr1.2:54436988-54437007 None:intergenic 20.0%
!!! CGTCAAATTTTAAAAAATGA+AGG + chr1.2:54436994-54437013 None:intergenic 20.0%
!!! GAACAGTATCTATTTTTATT+TGG - chr1.2:54436780-54436799 MS.gene50562:intron 20.0%
!!! TTGTAATTTTCTACTTAGTT+TGG - chr1.2:54435333-54435352 MS.gene50562:intron 20.0%
!!! TTTTAACAGAAGCTAATTAT+AGG + chr1.2:54436452-54436471 None:intergenic 20.0%
!!! TTTTTTGTAAGAGATTAGAT+GGG - chr1.2:54435236-54435255 MS.gene50562:intron 20.0%
!!! TTTTTTTGTAAGAGATTAGA+TGG - chr1.2:54435235-54435254 MS.gene50562:intron 20.0%
! AACAATCATGCATCAAAATT+GGG + chr1.2:54435697-54435716 None:intergenic 25.0%
! ACAACTCTAACAAAAAAACT+AGG + chr1.2:54437145-54437164 None:intergenic 25.0%
! ACAAGATACTAAATTTCTTC+AGG - chr1.2:54434661-54434680 MS.gene50562:intron 25.0%
! ATTTCCTTCCAAAGTATATA+TGG - chr1.2:54435929-54435948 MS.gene50562:intron 25.0%
! CATTCAATAACTCATGTATT+TGG + chr1.2:54435057-54435076 None:intergenic 25.0%
! CTGATATAGATAATACTTGT+TGG - chr1.2:54437072-54437091 MS.gene50562:intron 25.0%
! TATATATGGAAAGTTGAAAC+CGG - chr1.2:54435943-54435962 MS.gene50562:intron 25.0%
! TCAACTTTCCATATATACTT+TGG + chr1.2:54435940-54435959 None:intergenic 25.0%
! TCTAACAAAAAAACTAGGAT+TGG + chr1.2:54437140-54437159 None:intergenic 25.0%
! TCTCCAATTTGAAAATGTTA+AGG + chr1.2:54435138-54435157 None:intergenic 25.0%
! TTAAGCTTATATTGGAATGT+TGG - chr1.2:54435764-54435783 MS.gene50562:intron 25.0%
!! AAGGCTTAAACAATTTTACT+TGG - chr1.2:54437216-54437235 MS.gene50562:intron 25.0%
!! AATTAGCTTCTGTTAAAAGT+TGG - chr1.2:54436454-54436473 MS.gene50562:intron 25.0%
!! AGTCCTTAACATTTTCAAAT+TGG - chr1.2:54435132-54435151 MS.gene50562:intron 25.0%
!! ATAGATAATACTTGTTGGAT+TGG - chr1.2:54437077-54437096 MS.gene50562:intron 25.0%
!! CATCAAAAGCAAGATAATTT+TGG + chr1.2:54436857-54436876 None:intergenic 25.0%
!! CTTTTGATGAAAATAGTAGA+GGG - chr1.2:54436868-54436887 MS.gene50562:intron 25.0%
!! TATATTGGAATGTTGGTTAA+CGG - chr1.2:54435771-54435790 MS.gene50562:intron 25.0%
!! TTTTGATGAAAATAGTAGAG+GGG - chr1.2:54436869-54436888 MS.gene50562:intron 25.0%
!! TTTTTGTAAGAGATTAGATG+GGG - chr1.2:54435237-54435256 MS.gene50562:intron 25.0%
!!! TCTTTGCTCTAGATTTTATA+TGG - chr1.2:54435291-54435310 MS.gene50562:intron 25.0%
!!! TTTTTTTCCTGAATGTATTC+AGG - chr1.2:54436522-54436541 MS.gene50562:intron 25.0%
AAAACTGTTAGGAACCAATT+TGG - chr1.2:54435208-54435227 MS.gene50562:intron 30.0%
AAACTGTTAGGAACCAATTT+GGG - chr1.2:54435209-54435228 MS.gene50562:intron 30.0%
AAATTTGACGTATTACGTAC+CGG - chr1.2:54437004-54437023 MS.gene50562:intron 30.0%
AATAGACCAACAAATTTGTG+TGG + chr1.2:54435371-54435390 None:intergenic 30.0%
AATATTGTGAGGAACCAAAA+TGG - chr1.2:54435267-54435286 MS.gene50562:intron 30.0%
AATTCTTCCTGAATACATTC+AGG + chr1.2:54436532-54436551 None:intergenic 30.0%
ACAACACATTGTACTAAATC+GGG + chr1.2:54436048-54436067 None:intergenic 30.0%
AGAATACAGAGTAAAGATGT+AGG + chr1.2:54437310-54437329 None:intergenic 30.0%
AGTTGATCTGATAATTGGTT+TGG - chr1.2:54435867-54435886 MS.gene50562:intron 30.0%
ATATTGTGAGGAACCAAAAT+GGG - chr1.2:54435268-54435287 MS.gene50562:intron 30.0%
CAACAATCATGCATCAAAAT+TGG + chr1.2:54435698-54435717 None:intergenic 30.0%
CTTTCCATATATACTTTGGA+AGG + chr1.2:54435936-54435955 None:intergenic 30.0%
GAAACACATATTGAACCATA+TGG - chr1.2:54436148-54436167 MS.gene50562:intron 30.0%
GACAACACATTGTACTAAAT+CGG + chr1.2:54436049-54436068 None:intergenic 30.0%
TAGGTAAGAGAAATTACTCT+GGG + chr1.2:54437291-54437310 None:intergenic 30.0%
TCAGGAAATAAATTGTACCA+CGG + chr1.2:54435001-54435020 None:intergenic 30.0%
TCTCAAATTATTAACGACGT+CGG + chr1.2:54434860-54434879 None:intergenic 30.0%
TGAATGTATTCAGGAAGAAT+TGG - chr1.2:54436531-54436550 MS.gene50562:intron 30.0%
TGCTTATTGTTTACAACAGA+AGG + chr1.2:54435566-54435585 None:intergenic 30.0%
TTAATGCCACACAAATTTGT+TGG - chr1.2:54435362-54435381 MS.gene50562:intron 30.0%
TTGTGCTACAATATGCTATA+AGG - chr1.2:54437197-54437216 MS.gene50562:intron 30.0%
! ATATGGTGTTTCTTGTTTCT+AGG - chr1.2:54435308-54435327 MS.gene50562:intron 30.0%
! ATCGAAGTTGATCTGATAAT+TGG - chr1.2:54435862-54435881 MS.gene50562:intron 30.0%
! GCTTTTGATGAAAATAGTAG+AGG - chr1.2:54436867-54436886 MS.gene50562:intron 30.0%
! TAACTAAAGGGTATAGATCT+TGG - chr1.2:54434737-54434756 MS.gene50562:intron 30.0%
! TTAGGACATTTTGCAAAGTT+GGG - chr1.2:54436407-54436426 MS.gene50562:intron 30.0%
! TTTTTATAAAAACGACCGGA+GGG - chr1.2:54435099-54435118 MS.gene50562:intron 30.0%
!! CTTTAGTTCTTTACACTATG+TGG - chr1.2:54435606-54435625 MS.gene50562:intron 30.0%
!! TATGGTGTTTCTTGTTTCTA+GGG - chr1.2:54435309-54435328 MS.gene50562:intron 30.0%
!! TGGTTCCTAACAGTTTTATA+CGG + chr1.2:54435205-54435224 None:intergenic 30.0%
!!! TTTTTTATAAAAACGACCGG+AGG - chr1.2:54435098-54435117 MS.gene50562:intron 30.0%
AAGTGAACAAAAGGGTCAAT+GGG + chr1.2:54437103-54437122 None:intergenic 35.0%
ACTAACAACCTATGATGCAT+AGG + chr1.2:54437044-54437063 None:intergenic 35.0%
AGATTTGACCAATGAGGTTA+TGG - chr1.2:54435459-54435478 MS.gene50562:intron 35.0%
AGCTGAAACAGAAACAACAA+GGG + chr1.2:54434573-54434592 None:intergenic 35.0%
ATTGTACTAAATCGGGTATC+AGG + chr1.2:54436041-54436060 None:intergenic 35.0%
CGAGAAAACAGAAAATGTGT+CGG - chr1.2:54436561-54436580 MS.gene50562:intron 35.0%
CTGACCATATATAGAGGAAT+GGG - chr1.2:54435482-54435501 MS.gene50562:intron 35.0%
GAATGTGAGTATTTAGCAAG+CGG - chr1.2:54436347-54436366 MS.gene50562:intron 35.0%
GTAGGTAAGAGAAATTACTC+TGG + chr1.2:54437292-54437311 None:intergenic 35.0%
GTCATCCGTATAAAACTGTT+AGG - chr1.2:54435197-54435216 MS.gene50562:intron 35.0%
TAAACCTCTTATGGGATAGA+TGG - chr1.2:54437393-54437412 MS.gene50562:CDS 35.0%
TACAATGTGTTGTCAAGTAG+TGG - chr1.2:54436055-54436074 MS.gene50562:intron 35.0%
TATGGGATAGATGGTTTGAA+GGG - chr1.2:54437402-54437421 MS.gene50562:CDS 35.0%
TATTTAGCAAGCGGTTATGT+TGG - chr1.2:54436356-54436375 MS.gene50562:intron 35.0%
TCTTTCTGATTTCCTTGCAA+AGG - chr1.2:54436267-54436286 MS.gene50562:intron 35.0%
TGTAGTAGATTTGACCAATG+AGG - chr1.2:54435453-54435472 MS.gene50562:intron 35.0%
TGTTGTATTCCTGCATTTGT+TGG - chr1.2:54436487-54436506 MS.gene50562:intron 35.0%
TTATGGCTGACCATATATAG+AGG - chr1.2:54435476-54435495 MS.gene50562:intron 35.0%
TTATGGGATAGATGGTTTGA+AGG - chr1.2:54437401-54437420 MS.gene50562:CDS 35.0%
TTCTCTGATAACAGAGTTCA+TGG - chr1.2:54437365-54437384 MS.gene50562:CDS 35.0%
TTTCCTTGCAAAGGTTAATG+TGG - chr1.2:54436276-54436295 MS.gene50562:intron 35.0%
! GTTAGGACATTTTGCAAAGT+TGG - chr1.2:54436406-54436425 MS.gene50562:intron 35.0%
! TTCTTTGTTATAATGAGCCG+AGG - chr1.2:54435166-54435185 MS.gene50562:intron 35.0%
! TTTGGTTATTTCGTCGTAGT+TGG - chr1.2:54436798-54436817 MS.gene50562:intron 35.0%
!! AGAAATTACTCTGGGATTAG+TGG + chr1.2:54437283-54437302 None:intergenic 35.0%
AAATCGGGTATCAGGAAAAG+CGG + chr1.2:54436033-54436052 None:intergenic 40.0%
AACCTATGATGCATAGGTAC+GGG + chr1.2:54437038-54437057 None:intergenic 40.0%
ACCACCCATTCCTCTATATA+TGG + chr1.2:54435489-54435508 None:intergenic 40.0%
ACCATATATAGAGGAATGGG+TGG - chr1.2:54435485-54435504 MS.gene50562:intron 40.0%
ACGGATGACAAATTAGTCCT+CGG + chr1.2:54435186-54435205 None:intergenic 40.0%
ATCCAACATCTACATGCTTG+CGG + chr1.2:54437437-54437456 None:intergenic 40.0%
ATGGACCTGTAAACCTCTTA+TGG - chr1.2:54437384-54437403 MS.gene50562:CDS 40.0%
CAAACCATCTATCCCATAAG+AGG + chr1.2:54437400-54437419 None:intergenic 40.0%
CAACCTATGATGCATAGGTA+CGG + chr1.2:54437039-54437058 None:intergenic 40.0%
CAAGTGAACAAAAGGGTCAA+TGG + chr1.2:54437104-54437123 None:intergenic 40.0%
CAGCTGAAACAGAAACAACA+AGG + chr1.2:54434574-54434593 None:intergenic 40.0%
CATCCCACAAGTGAACAAAA+GGG + chr1.2:54437111-54437130 None:intergenic 40.0%
CTATCCCATAAGAGGTTTAC+AGG + chr1.2:54437392-54437411 None:intergenic 40.0%
CTCCAAACATGGATAAGTAC+CGG - chr1.2:54436704-54436723 MS.gene50562:intron 40.0%
GAACCACATTAACCTTTGCA+AGG + chr1.2:54436282-54436301 None:intergenic 40.0%
GAGTGAAGAACTATGAAGCA+CGG - chr1.2:54434799-54434818 MS.gene50562:intron 40.0%
GATACTGCGACTGATAAAAG+AGG - chr1.2:54434602-54434621 MS.gene50562:intron 40.0%
GATTACCAACCAACAAATGC+AGG + chr1.2:54436499-54436518 None:intergenic 40.0%
GCTGACCATATATAGAGGAA+TGG - chr1.2:54435481-54435500 MS.gene50562:intron 40.0%
TACCGGTACTTATCCATGTT+TGG + chr1.2:54436709-54436728 None:intergenic 40.0%
TCATCCCACAAGTGAACAAA+AGG + chr1.2:54437112-54437131 None:intergenic 40.0%
TCTGTTCGTGCTAGAAGTTT+CGG - chr1.2:54436171-54436190 MS.gene50562:intron 40.0%
TGGACCTGTAAACCTCTTAT+GGG - chr1.2:54437385-54437404 MS.gene50562:CDS 40.0%
TTCGGTGAAAGCTGTGATAT+AGG - chr1.2:54436189-54436208 MS.gene50562:intron 40.0%
! GTATTCCTGCATTTGTTGGT+TGG - chr1.2:54436491-54436510 MS.gene50562:intron 40.0%
! TGACCCTTTTGTTCACTTGT+GGG - chr1.2:54437105-54437124 MS.gene50562:intron 40.0%
! TTGACCCTTTTGTTCACTTG+TGG - chr1.2:54437104-54437123 MS.gene50562:intron 40.0%
!! AAATGTGTCGGCTATATGCA+AGG - chr1.2:54436573-54436592 MS.gene50562:intron 40.0%
!! AAGCTTTTTCTGCTGATGTG+TGG - chr1.2:54435805-54435824 MS.gene50562:intron 40.0%
!! AGTTGTTAGCTCTGAAGCAT+GGG - chr1.2:54436679-54436698 MS.gene50562:intron 40.0%
!! TAGTTGTTAGCTCTGAAGCA+TGG - chr1.2:54436678-54436697 MS.gene50562:intron 40.0%
!! TCTAGAGCAAAGACCCATTT+TGG + chr1.2:54435284-54435303 None:intergenic 40.0%
AAACCGGCGTTGTCATATAC+AGG - chr1.2:54435959-54435978 MS.gene50562:intron 45.0%
AAGCATGGGTACTCCAAACA+TGG - chr1.2:54436693-54436712 MS.gene50562:intron 45.0%
ACTGGGACATGCTTAATTCG+AGG - chr1.2:54434934-54434953 MS.gene50562:intron 45.0%
ATCCGCAAGCATGTAGATGT+TGG - chr1.2:54437432-54437451 MS.gene50562:CDS 45.0%
ATGATGCATAGGTACGGGTA+CGG + chr1.2:54437033-54437052 None:intergenic 45.0%
ATGGTCAGCCATAACCTCAT+TGG + chr1.2:54435470-54435489 None:intergenic 45.0%
GCGCAGATTGCTACAAAACA+TGG - chr1.2:54437243-54437262 MS.gene50562:intron 45.0%
GTTGGTGCAGCAAAAACGTA+CGG - chr1.2:54436816-54436835 MS.gene50562:intron 45.0%
TAAGAGATTAGATGGGGTCG+CGG - chr1.2:54435243-54435262 MS.gene50562:intron 45.0%
TACCCGTACCTATGCATCAT+AGG - chr1.2:54437033-54437052 MS.gene50562:intron 45.0%
TCTAGCACGAACAGACCATA+TGG + chr1.2:54436166-54436185 None:intergenic 45.0%
TGAAGCACGGATACAAACAC+CGG - chr1.2:54434812-54434831 MS.gene50562:intron 45.0%
! AAATGAAGGTACGGGTACGT+TGG + chr1.2:54436980-54436999 None:intergenic 45.0%
! GGACTAGTAATAGTTCCCTC+CGG + chr1.2:54435117-54435136 None:intergenic 45.0%
!! ACATGGATAAGTACCGGTAC+CGG - chr1.2:54436710-54436729 MS.gene50562:intron 45.0%
!! ATAGCGCTGAAGCACTGATA+CGG + chr1.2:54435985-54436004 None:intergenic 45.0%
!! GTCAGTATCAGTGTCGTTTC+CGG + chr1.2:54434834-54434853 None:intergenic 45.0%
!! TACTCTGGGATTAGTGGATC+AGG + chr1.2:54437277-54437296 None:intergenic 45.0%
AAGAGATTAGATGGGGTCGC+GGG - chr1.2:54435244-54435263 MS.gene50562:intron 50.0%
ACCACGGTAAAGACCAGACT+GGG + chr1.2:54434985-54435004 None:intergenic 50.0%
CATGACACGTGTTCGACACT+GGG - chr1.2:54434917-54434936 MS.gene50562:intron 50.0%
TACCACGGTAAAGACCAGAC+TGG + chr1.2:54434986-54435005 None:intergenic 50.0%
TAGATGGTTTGAAGGGCCTC+AGG - chr1.2:54437409-54437428 MS.gene50562:CDS 50.0%
TCATAGGTGAAGACCCAGTC+TGG - chr1.2:54434969-54434988 MS.gene50562:intron 50.0%
TCATGACACGTGTTCGACAC+TGG - chr1.2:54434916-54434935 MS.gene50562:intron 50.0%
TGATGCATAGGTACGGGTAC+GGG + chr1.2:54437032-54437051 None:intergenic 50.0%
!! CATGGATAAGTACCGGTACC+GGG - chr1.2:54436711-54436730 MS.gene50562:intron 50.0%
ACGAGTACCATACGCGTACC+CGG + chr1.2:54436749-54436768 None:intergenic 55.0%
ATAGGTACGGGTACGGGTAC+CGG + chr1.2:54437026-54437045 None:intergenic 55.0%
CGGCCTGTATATGACAACGC+CGG + chr1.2:54435965-54435984 None:intergenic 55.0%
CTACATGCTTGCGGATCCTG+AGG + chr1.2:54437428-54437447 None:intergenic 55.0%
TACCATACGCGTACCCGGTA+CGG + chr1.2:54436744-54436763 None:intergenic 55.0%
! ACCCAGTCTGGTCTTTACCG+TGG - chr1.2:54434981-54435000 MS.gene50562:intron 55.0%
!! GAGGAGCGTCTGTGCTTCAT+AGG - chr1.2:54434953-54434972 MS.gene50562:intron 55.0%
ACCATACGCGTACCCGGTAC+GGG + chr1.2:54436743-54436762 None:intergenic 60.0%
GGGGTCGCGGGAATATTGTG+AGG - chr1.2:54435256-54435275 MS.gene50562:intron 60.0%
! GTCTGAAACGGCGTCGTCTG+CGG - chr1.2:54434532-54434551 MS.gene50562:CDS 60.0%
ACCCGTACCGGGTACGCGTA+TGG - chr1.2:54436739-54436758 MS.gene50562:intron 65.0%
TACCGGGTACGCACCCGTAC+CGG - chr1.2:54436727-54436746 MS.gene50562:intron 65.0%
TACGGGTGCGTACCCGGTAC+CGG + chr1.2:54436726-54436745 None:intergenic 65.0%
ACCCGGTACGGGTGCGTACC+CGG + chr1.2:54436732-54436751 None:intergenic 70.0%
ACCGGGTACGCACCCGTACC+GGG - chr1.2:54436728-54436747 MS.gene50562:intron 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 54434527 54437463 54434527 ID=MS.gene50562
chr1.2 mRNA 54434527 54437463 54434527 ID=MS.gene50562.t1;Parent=MS.gene50562
chr1.2 exon 54437308 54437463 54437308 ID=MS.gene50562.t1.exon1;Parent=MS.gene50562.t1
chr1.2 CDS 54437308 54437463 54437308 ID=cds.MS.gene50562.t1;Parent=MS.gene50562.t1
chr1.2 exon 54435394 54435446 54435394 ID=MS.gene50562.t1.exon2;Parent=MS.gene50562.t1
chr1.2 CDS 54435394 54435446 54435394 ID=cds.MS.gene50562.t1;Parent=MS.gene50562.t1
chr1.2 exon 54434697 54434741 54434697 ID=MS.gene50562.t1.exon3;Parent=MS.gene50562.t1
chr1.2 CDS 54434697 54434741 54434697 ID=cds.MS.gene50562.t1;Parent=MS.gene50562.t1
chr1.2 exon 54434527 54434611 54434527 ID=MS.gene50562.t1.exon4;Parent=MS.gene50562.t1
chr1.2 CDS 54434527 54434611 54434527 ID=cds.MS.gene50562.t1;Parent=MS.gene50562.t1
Gene Sequence

>MS.gene50562

ATGGCGTCTGAAACGGCGTCGTCTGCGGATGAAGAAAAAGAAACCCTTGTTGTTTCTGTTTCAGCTGCTGTAGCTGATACTGCGACTGATAAAAGAGGAAAGCATCGTATTCTTGCTGAATTGAAACGTCTTCAACAAGATACTAAATTTCTTCAGGTTTGTTTGTTAATTACATTCATCGTGTTTCTTTTTTGAATTGTGTATAATGTATAACTAAAGGGTATAGATCTTGGTAATAAATTAGTTTCACAGTTTCAAAGATTTTAGATTTAGAGTGAAGAACTATGAAGCACGGATACAAACACCGGAAACGACACTGATACTGACACGCCGACGTCGTTAATAATTTGAGAAAATCACATAATTCAATGTAATTATATGTGTTTGTGTCATGACACGTGTTCGACACTGGGACATGCTTAATTCGAGGAGCGTCTGTGCTTCATAGGTGAAGACCCAGTCTGGTCTTTACCGTGGTACAATTTATTTCCTGATAAAAATAAAATTCTTTAACATTTTAACATAGACCAAATACATGAGTTATTGAATGAATATAAAAAGTGAATTGTTTTTTTTTATAAAAACGACCGGAGGGAACTATTACTAGTCCTTAACATTTTCAAATTGGAGAAAAATTAGTTCTTTGTTATAATGAGCCGAGGACTAATTTGTCATCCGTATAAAACTGTTAGGAACCAATTTGGGTTTTTTTTTTGTAAGAGATTAGATGGGGTCGCGGGAATATTGTGAGGAACCAAAATGGGTCTTTGCTCTAGATTTTATATGGTGTTTCTTGTTTCTAGGGTTTGTAATTTTCTACTTAGTTTGGCACATTTTAATGCCACACAAATTTGTTGGTCTATTTGAAATAGAGCAAATGCTATTTGCGACGAAAAGTTTATACAAGAAAAATAAGATTGCATAAATGTAGTAGATTTGACCAATGAGGTTATGGCTGACCATATATAGAGGAATGGGTGGTTATTATTTGTAAGTGTTACATGCTGTCCCATAGTATTTATGTGTTTTTTGTCAACCTTCTGTTGTAAACAATAAGCATTTTCCTTTGAATTTATGATCTTTAGTTCTTTACACTATGTGGACTCTTAGTTGTATTGTATTGATAAATTACAAAACAATGTGGGATTATAAAGTTGAGAAGATTAACCCAATTTTGATGCATGATTGTTGTCTTAGTGTTGTAATGTCAAAAACACTCGTGTTTTCACTTTATAGCTTAAGCTTATATTGGAATGTTGGTTAACGGTCACAGAATCAAAGCTTTTTCTGCTGATGTGTGGTCTAAAGTTTGATTCTTGCTGTCATTATAGCGATATCGAAGTTGATCTGATAATTGGTTTGGTTCATTTATAGCATGCGTATCTTCAGAGTAGATATGCATATTTCCTTCCAAAGTATATATGGAAAGTTGAAACCGGCGTTGTCATATACAGGCCGTATCAGTGCTTCAGCGCTATAGTATAGCAGAATTTTGATAAAACGCCGCTTTTCCTGATACCCGATTTAGTACAATGTGTTGTCAAGTAGTGGTTAGCATTGCAGCATAGTAGAATTTGAACAATCTGCTATTTACTGCAATTTGCGATAGACAACACTAGTTGAAACACATATTGAACCATATGGTCTGTTCGTGCTAGAAGTTTCGGTGAAAGCTGTGATATAGGAGAGCAAAATTCAAAATAAACTTAACTTATAGCGGACTAAAATTTGTGTCACGTGTCTTTCTGATTTCCTTGCAAAGGTTAATGTGGTTCAGTCGTTTAGTTTCCATACATTTTTTCTTTTTTGATTCTTGTCATGAATGTGAGTATTTAGCAAGCGGTTATGTTGGTTGACTTTTCTAATTGCTTAAATCAATGTTAGGACATTTTGCAAAGTTGGGTGTATTCTTGTAGTGTAAGCCTATAATTAGCTTCTGTTAAAAGTTGGTTGTCTCTACTGTTGTATTCCTGCATTTGTTGGTTGGTAATCATTTTTTTTTCCTGAATGTATTCAGGAAGAATTGGATGAGCTCGAGAAAACAGAAAATGTGTCGGCTATATGCAAGGAGTAAGTACTGACTGAACAGTTGTTTCTCTCTTACATGAAAGATATTAGAAAATTATGATATATAGTTCAAGAGAGAATTTTTAGTTGTTAGCTCTGAAGCATGGGTACTCCAAACATGGATAAGTACCGGTACCGGGTACGCACCCGTACCGGGTACGCGTATGGTACTCGTAATTTAGTACCGAACAGTATCTATTTTTATTTGGTTATTTCGTCGTAGTTGGTGCAGCAAAAACGTACGGTCGTTGAAAAAATAACCAAAATTATCTTGCTTTTGATGAAAATAGTAGAGGGGATGCACCAAAAAAATAAAACTCTCAAGTAACATGATGATAGAAGTGTAGTAATTCACTTGAAAAATATTTTTTTGATGTTTTATACCAACGTACCCGTACCTTCATTTTTTAAAATTTGACGTATTACGTACCGGTACCCGTACCCGTACCTATGCATCATAGGTTGTTAGTTAGAACATCTGATATAGATAATACTTGTTGGATTGGCCCATTGACCCTTTTGTTCACTTGTGGGATGACTGCACCAATCCTAGTTTTTTTGTTAGAGTTGTTAAAAATTAAAATGCTTATCTCATTATTAGTATTGTGCTACAATATGCTATAAGGCTTAAACAATTTTACTTGGCATTGCGCAGATTGCTACAAAACATGGATAGTAGACCTGATCCACTAATCCCAGAGTAATTTCTCTTACCTACATCTTTACTCTGTATTCTTTCTTGATTGTGAGTTTAAAGAGTAATCGTCAGTTTTCTCTGATAACAGAGTTCATGGACCTGTAAACCTCTTATGGGATAGATGGTTTGAAGGGCCTCAGGATCCGCAAGCATGTAGATGTTGGATACTTTGA
Protein sequence

>MS.gene50562.t1

MASETASSADEEKETLVVSVSAAVADTATDKRGKHRILAELKRLQQDTKFLQEELDELEKTENVSAICKELLQNMDSRPDPLIPEVHGPVNLLWDRWFEGPQDPQACRCWIL