Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50754.t1 | XP_003606962.1 | 90.7 | 214 | 19 | 1 | 1 | 214 | 1 | 213 | 3.20E-93 | 351.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50754.t1 | G7JHE9 | 90.7 | 214 | 19 | 1 | 1 | 214 | 1 | 213 | 2.3e-93 | 351.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055358 | MS.gene50754 | 0.814262 | 1.68E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50754.t1 | MTR_4g070640 | 90.654 | 214 | 19 | 1 | 1 | 214 | 1 | 213 | 5.52e-125 | 352 |
| MS.gene50754.t1 | MTR_4g070660 | 74.779 | 226 | 44 | 4 | 1 | 214 | 1 | 225 | 1.37e-103 | 298 |
| MS.gene50754.t1 | MTR_4g070630 | 65.882 | 170 | 56 | 2 | 46 | 214 | 1 | 169 | 3.74e-71 | 214 |
| MS.gene50754.t1 | MTR_3g117050 | 39.044 | 251 | 95 | 12 | 9 | 209 | 10 | 252 | 1.79e-27 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50754.t1 | AT5G66440 | 28.750 | 240 | 136 | 6 | 1 | 209 | 1 | 236 | 8.01e-23 | 92.8 |
Find 41 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACTCAAGTGAGTATTTA+AGG | 0.169279 | 4.3:-34393460 | MS.gene50754:CDS |
| AATCATCACGTTGTTTGTTC+AGG | 0.261006 | 4.3:-34393381 | MS.gene50754:CDS |
| TCATCGTGTTCAGGTTCTTT+TGG | 0.272939 | 4.3:+34393341 | None:intergenic |
| CACTTCAACGAGTCGTATTA+AGG | 0.283376 | 4.3:+34393538 | None:intergenic |
| TAGGATTGCGTTGGCTTTCT+CGG | 0.305380 | 4.3:+34393589 | None:intergenic |
| AAGAAACGACGTCGTGTATT+GGG | 0.312077 | 4.3:-34393161 | MS.gene50754:CDS |
| ACGTTGTTTGTTCAGGGTTC+TGG | 0.316731 | 4.3:-34393374 | MS.gene50754:CDS |
| TCGTTGCTCATTTCATCTTC+CGG | 0.342235 | 4.3:+34393056 | None:intergenic |
| TTGCGTTGGCTTTCTCGGCT+TGG | 0.357630 | 4.3:+34393594 | None:intergenic |
| TCGTAAATATCATCGTGTTC+AGG | 0.373649 | 4.3:+34393332 | None:intergenic |
| AGAGAATAATAAAAGATGAT+AGG | 0.379532 | 4.3:-34393271 | MS.gene50754:CDS |
| GAAGAAACGACGTCGTGTAT+TGG | 0.380853 | 4.3:-34393162 | MS.gene50754:CDS |
| AGGTGAAGAAGAGTTATAGA+AGG | 0.390915 | 4.3:-34393202 | MS.gene50754:CDS |
| CTTGAGTTTCTTACTACAAC+TGG | 0.398117 | 4.3:+34393473 | None:intergenic |
| TCCTGCCGTTGAGGAGGAAA+TGG | 0.401664 | 4.3:-34393093 | MS.gene50754:CDS |
| ATCATCACGTTGTTTGTTCA+GGG | 0.409938 | 4.3:-34393380 | MS.gene50754:CDS |
| AATAAAAGATGATAGGATAA+TGG | 0.411065 | 4.3:-34393264 | MS.gene50754:CDS |
| AATGGTGAGGATTTCGTATC+CGG | 0.424985 | 4.3:-34393075 | MS.gene50754:CDS |
| AAACTCAAGTGAGTATTTAA+GGG | 0.440864 | 4.3:-34393459 | MS.gene50754:CDS |
| CGTTGGCTTTCTCGGCTTGG+AGG | 0.445821 | 4.3:+34393597 | None:intergenic |
| TCTCGGCTTGGAGGTTAAAA+CGG | 0.449727 | 4.3:+34393606 | None:intergenic |
| TGAGTATTTAAGGGACGTTT+CGG | 0.464899 | 4.3:-34393450 | MS.gene50754:CDS |
| AAAGCAACAAAGCGGATTGA+AGG | 0.496705 | 4.3:-34393000 | MS.gene50754:CDS |
| ACCATTTCCTCCTCAACGGC+AGG | 0.502183 | 4.3:+34393092 | None:intergenic |
| TAAAACACCACAAGCTTCAA+AGG | 0.505796 | 4.3:-34393568 | MS.gene50754:CDS |
| GCAAAAGAAAATGAAAAGAG+AGG | 0.527440 | 4.3:-34393222 | MS.gene50754:CDS |
| TTGTGACCCTTTGAAGCTTG+TGG | 0.528150 | 4.3:+34393561 | None:intergenic |
| CGCTACTCCTGCCGTTGAGG+AGG | 0.529548 | 4.3:-34393099 | MS.gene50754:CDS |
| AAGCAACAAAGCGGATTGAA+GGG | 0.545105 | 4.3:-34392999 | MS.gene50754:CDS |
| TGAGTTTCGTCGCATTGTAG+AGG | 0.545398 | 4.3:-34393033 | MS.gene50754:CDS |
| AGCCGCTACTCCTGCCGTTG+AGG | 0.549603 | 4.3:-34393102 | MS.gene50754:CDS |
| AAAACACCACAAGCTTCAAA+GGG | 0.570187 | 4.3:-34393567 | MS.gene50754:CDS |
| GATGATAGGATAATGGAAGA+AGG | 0.570977 | 4.3:-34393257 | MS.gene50754:CDS |
| GATAGGATAATGGAAGAAGG+TGG | 0.576977 | 4.3:-34393254 | MS.gene50754:CDS |
| CTCCTCAACGGCAGGAGTAG+CGG | 0.587462 | 4.3:+34393100 | None:intergenic |
| GGTGTCAGAGTGAGAACGAA+AGG | 0.592349 | 4.3:-34393136 | MS.gene50754:CDS |
| CCTCACCATTTCCTCCTCAA+CGG | 0.636501 | 4.3:+34393088 | None:intergenic |
| AACGACGTCGTGTATTGGGA+AGG | 0.637926 | 4.3:-34393157 | MS.gene50754:CDS |
| AGCAACAAAGCGGATTGAAG+GGG | 0.669693 | 4.3:-34392998 | MS.gene50754:CDS |
| TTTATTGCAAAGCAACAAAG+CGG | 0.681337 | 4.3:-34393008 | MS.gene50754:CDS |
| CCGTTGAGGAGGAAATGGTG+AGG | 0.699677 | 4.3:-34393088 | MS.gene50754:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAAGATGATAGGATAA+TGG | - | chr4.3:34393348-34393367 | MS.gene50754:CDS | 20.0% |
| !! | AGAGAATAATAAAAGATGAT+AGG | - | chr4.3:34393341-34393360 | MS.gene50754:CDS | 20.0% |
| !! | CGATAACAAAAACAAAATAA+TGG | + | chr4.3:34393201-34393220 | None:intergenic | 20.0% |
| ! | AAACTCAAGTGAGTATTTAA+GGG | - | chr4.3:34393153-34393172 | MS.gene50754:CDS | 25.0% |
| !!! | TTGTTTTAGTCTTGATTTCA+AGG | - | chr4.3:34393101-34393120 | MS.gene50754:CDS | 25.0% |
| GAAACTCAAGTGAGTATTTA+AGG | - | chr4.3:34393152-34393171 | MS.gene50754:CDS | 30.0% | |
| GCAAAAGAAAATGAAAAGAG+AGG | - | chr4.3:34393390-34393409 | MS.gene50754:CDS | 30.0% | |
| TTTATTGCAAAGCAACAAAG+CGG | - | chr4.3:34393604-34393623 | MS.gene50754:CDS | 30.0% | |
| AAAACACCACAAGCTTCAAA+GGG | - | chr4.3:34393045-34393064 | MS.gene50754:CDS | 35.0% | |
| AATCATCACGTTGTTTGTTC+AGG | - | chr4.3:34393231-34393250 | MS.gene50754:CDS | 35.0% | |
| AGGTGAAGAAGAGTTATAGA+AGG | - | chr4.3:34393410-34393429 | MS.gene50754:CDS | 35.0% | |
| ATCATCACGTTGTTTGTTCA+GGG | - | chr4.3:34393232-34393251 | MS.gene50754:CDS | 35.0% | |
| CTTGAGTTTCTTACTACAAC+TGG | + | chr4.3:34393142-34393161 | None:intergenic | 35.0% | |
| TAAAACACCACAAGCTTCAA+AGG | - | chr4.3:34393044-34393063 | MS.gene50754:CDS | 35.0% | |
| TCGTAAATATCATCGTGTTC+AGG | + | chr4.3:34393283-34393302 | None:intergenic | 35.0% | |
| TGAGTATTTAAGGGACGTTT+CGG | - | chr4.3:34393162-34393181 | MS.gene50754:CDS | 35.0% | |
| !! | GATGATAGGATAATGGAAGA+AGG | - | chr4.3:34393355-34393374 | MS.gene50754:CDS | 35.0% |
| !! | TTTGAAGCTTGTGGTGTTTT+AGG | + | chr4.3:34393045-34393064 | None:intergenic | 35.0% |
| AAGAAACGACGTCGTGTATT+GGG | - | chr4.3:34393451-34393470 | MS.gene50754:CDS | 40.0% | |
| AATGGTGAGGATTTCGTATC+CGG | - | chr4.3:34393537-34393556 | MS.gene50754:CDS | 40.0% | |
| CACTTCAACGAGTCGTATTA+AGG | + | chr4.3:34393077-34393096 | None:intergenic | 40.0% | |
| TCGTTGCTCATTTCATCTTC+CGG | + | chr4.3:34393559-34393578 | None:intergenic | 40.0% | |
| !! | AAAGCAACAAAGCGGATTGA+AGG | - | chr4.3:34393612-34393631 | MS.gene50754:CDS | 40.0% |
| !! | AAGCAACAAAGCGGATTGAA+GGG | - | chr4.3:34393613-34393632 | MS.gene50754:CDS | 40.0% |
| !! | GATAGGATAATGGAAGAAGG+TGG | - | chr4.3:34393358-34393377 | MS.gene50754:CDS | 40.0% |
| !! | TCATCGTGTTCAGGTTCTTT+TGG | + | chr4.3:34393274-34393293 | None:intergenic | 40.0% |
| ACGTTGTTTGTTCAGGGTTC+TGG | - | chr4.3:34393238-34393257 | MS.gene50754:CDS | 45.0% | |
| GAAGAAACGACGTCGTGTAT+TGG | - | chr4.3:34393450-34393469 | MS.gene50754:CDS | 45.0% | |
| TGAGTTTCGTCGCATTGTAG+AGG | - | chr4.3:34393579-34393598 | MS.gene50754:CDS | 45.0% | |
| !! | AGCAACAAAGCGGATTGAAG+GGG | - | chr4.3:34393614-34393633 | MS.gene50754:CDS | 45.0% |
| !! | TAGGATTGCGTTGGCTTTCT+CGG | + | chr4.3:34393026-34393045 | None:intergenic | 45.0% |
| !! | TCTCGGCTTGGAGGTTAAAA+CGG | + | chr4.3:34393009-34393028 | None:intergenic | 45.0% |
| !! | TTGTGACCCTTTGAAGCTTG+TGG | + | chr4.3:34393054-34393073 | None:intergenic | 45.0% |
| !!! | GTGGTGTTTTAGGATTGCGT+TGG | + | chr4.3:34393035-34393054 | None:intergenic | 45.0% |
| AACGACGTCGTGTATTGGGA+AGG | - | chr4.3:34393455-34393474 | MS.gene50754:CDS | 50.0% | |
| CCTCACCATTTCCTCCTCAA+CGG | + | chr4.3:34393527-34393546 | None:intergenic | 50.0% | |
| GGTGTCAGAGTGAGAACGAA+AGG | - | chr4.3:34393476-34393495 | MS.gene50754:CDS | 50.0% | |
| ACCATTTCCTCCTCAACGGC+AGG | + | chr4.3:34393523-34393542 | None:intergenic | 55.0% | |
| CCGTTGAGGAGGAAATGGTG+AGG | - | chr4.3:34393524-34393543 | MS.gene50754:CDS | 55.0% | |
| TCCTGCCGTTGAGGAGGAAA+TGG | - | chr4.3:34393519-34393538 | MS.gene50754:CDS | 55.0% | |
| !! | TTGCGTTGGCTTTCTCGGCT+TGG | + | chr4.3:34393021-34393040 | None:intergenic | 55.0% |
| CTCCTCAACGGCAGGAGTAG+CGG | + | chr4.3:34393515-34393534 | None:intergenic | 60.0% | |
| !! | CGTTGGCTTTCTCGGCTTGG+AGG | + | chr4.3:34393018-34393037 | None:intergenic | 60.0% |
| AGCCGCTACTCCTGCCGTTG+AGG | - | chr4.3:34393510-34393529 | MS.gene50754:CDS | 65.0% | |
| CGCTACTCCTGCCGTTGAGG+AGG | - | chr4.3:34393513-34393532 | MS.gene50754:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 34392995 | 34393639 | 34392995 | ID=MS.gene50754 |
| chr4.3 | mRNA | 34392995 | 34393639 | 34392995 | ID=MS.gene50754.t1;Parent=MS.gene50754 |
| chr4.3 | exon | 34392995 | 34393639 | 34392995 | ID=MS.gene50754.t1.exon1;Parent=MS.gene50754.t1 |
| chr4.3 | CDS | 34392995 | 34393639 | 34392995 | ID=cds.MS.gene50754.t1;Parent=MS.gene50754.t1 |
| Gene Sequence |
| Protein sequence |
