Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50824.t1 | XP_003597994.1 | 87.4 | 262 | 13 | 1 | 39 | 280 | 41 | 302 | 1.40E-127 | 466.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50824.t1 | Q7TP52 | 26.2 | 195 | 130 | 6 | 104 | 297 | 22 | 203 | 7.6e-13 | 76.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50824.t1 | G7IYB4 | 87.4 | 262 | 13 | 1 | 39 | 280 | 41 | 302 | 1.0e-127 | 466.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060122 | MS.gene50824 | 0.806877 | 6.64E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50824.t1 | MTR_3g005060 | 88.411 | 302 | 13 | 2 | 1 | 280 | 1 | 302 | 1.95e-167 | 474 |
| MS.gene50824.t1 | MTR_3g005060 | 84.106 | 302 | 12 | 3 | 1 | 280 | 1 | 288 | 1.36e-153 | 438 |
| MS.gene50824.t1 | MTR_3g005050 | 88.679 | 106 | 4 | 1 | 341 | 446 | 1 | 98 | 6.34e-59 | 188 |
| MS.gene50824.t1 | MTR_3g005050 | 88.119 | 101 | 4 | 1 | 341 | 441 | 1 | 93 | 1.82e-55 | 179 |
| MS.gene50824.t1 | MTR_1g052490 | 68.182 | 110 | 32 | 2 | 153 | 259 | 7 | 116 | 1.01e-48 | 162 |
| MS.gene50824.t1 | MTR_4g117380 | 32.727 | 110 | 68 | 3 | 127 | 236 | 37 | 140 | 2.34e-11 | 63.9 |
| MS.gene50824.t1 | MTR_4g117450 | 28.235 | 170 | 96 | 6 | 132 | 280 | 42 | 206 | 2.91e-11 | 63.9 |
| MS.gene50824.t1 | MTR_4g117400 | 32.673 | 101 | 63 | 3 | 131 | 231 | 95 | 190 | 6.86e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50824.t1 | AT1G35420 | 51.232 | 203 | 75 | 2 | 98 | 280 | 45 | 243 | 7.85e-71 | 225 |
| MS.gene50824.t1 | AT1G35420 | 51.232 | 203 | 75 | 2 | 98 | 280 | 81 | 279 | 1.30e-70 | 226 |
| MS.gene50824.t1 | AT1G35420 | 51.232 | 203 | 75 | 2 | 98 | 280 | 86 | 284 | 2.10e-70 | 226 |
| MS.gene50824.t1 | AT1G72170 | 64.000 | 100 | 32 | 2 | 341 | 437 | 1 | 99 | 9.64e-36 | 128 |
| MS.gene50824.t1 | AT1G22520 | 68.966 | 87 | 23 | 1 | 354 | 436 | 12 | 98 | 3.20e-35 | 126 |
| MS.gene50824.t1 | AT1G22520 | 67.816 | 87 | 24 | 1 | 354 | 436 | 12 | 98 | 1.94e-33 | 124 |
Find 102 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCTTCTTTCTGATGTTTA+TGG | 0.109167 | 3.4:-846282 | MS.gene50824:CDS |
| TCCAGAAAACTTGGCATAAT+TGG | 0.171939 | 3.4:-845938 | MS.gene50824:CDS |
| CCTGCTTCCTCTCTCTTTAC+TGG | 0.185506 | 3.4:+844620 | None:intergenic |
| CGCTGGGCTTCTATTGCTTT+TGG | 0.217123 | 3.4:-838941 | MS.gene50824:CDS |
| TGCTTCTTTCTGATGTTTAT+GGG | 0.222443 | 3.4:-846281 | MS.gene50824:CDS |
| TTCGGTTGTGCAAGCTTTGC+TGG | 0.240747 | 3.4:+838860 | None:intergenic |
| ATCTATCCTTGTACCATAAA+AGG | 0.252752 | 3.4:+845841 | None:intergenic |
| GGACACTGTGTCATAGCTTA+TGG | 0.265692 | 3.4:-844679 | MS.gene50824:CDS |
| GAATGTTCTCGTCTGTTTGA+TGG | 0.310140 | 3.4:-838887 | MS.gene50824:CDS |
| CAAAGCAAGCATCTTGGTCT+TGG | 0.330779 | 3.4:+845876 | None:intergenic |
| TTCTGATGTTTATGGGTTTG+AGG | 0.335907 | 3.4:-846274 | MS.gene50824:CDS |
| TGGAGAGGAGCTTTATTTAG+AGG | 0.337897 | 3.4:-846370 | MS.gene50824:CDS |
| GACACTGTGTCATAGCTTAT+GGG | 0.380665 | 3.4:-844678 | MS.gene50824:CDS |
| CATTGTTCAAACTGAGTGTT+TGG | 0.394017 | 3.4:+846049 | None:intergenic |
| CAAGACCAAGATGCTTGCTT+TGG | 0.399499 | 3.4:-845875 | MS.gene50824:CDS |
| TGGAGGAGGCAAAGTGTTAG+AGG | 0.399979 | 3.4:-845906 | MS.gene50824:CDS |
| TTTGGTGCTGGAGTGGGAAT+AGG | 0.400010 | 3.4:-838923 | MS.gene50824:CDS |
| TAGGGAGTCCTCAGACTCGC+TGG | 0.408022 | 3.4:-838958 | MS.gene50824:CDS |
| CAGTTTGAACAATGGATTGC+TGG | 0.409924 | 3.4:-846040 | MS.gene50824:CDS |
| AAAGAGAGGTTGGAAGGAAA+TGG | 0.413388 | 3.4:+848287 | None:intergenic |
| GTAGGAGCCTCTGAGGCCTT+AGG | 0.414119 | 3.4:+838818 | None:intergenic |
| TCCAACTCGTTCCGTTCCGA+TGG | 0.420966 | 3.4:-844411 | MS.gene50824:CDS |
| TCAGATGTGATAGTTTAATT+CGG | 0.422245 | 3.4:-844649 | MS.gene50824:CDS |
| ATCAAGGCAGGCATCCCATT+TGG | 0.436230 | 3.4:+844343 | None:intergenic |
| AGAGCAACTGTAAGCATTTG+TGG | 0.439788 | 3.4:-848370 | MS.gene50824:CDS |
| AAACATCAGAAAGAAGCAAT+AGG | 0.452098 | 3.4:+846286 | None:intergenic |
| CAAGATGCTTGCTTTGGCAC+TGG | 0.457644 | 3.4:-845869 | MS.gene50824:CDS |
| TTTGGCTGGTCCTTTGTCCA+TGG | 0.461066 | 3.4:+846067 | None:intergenic |
| AGTATGACGTCGATGCCAAA+TGG | 0.464332 | 3.4:-844358 | MS.gene50824:CDS |
| ATTCAGACTACAATTGATAG+TGG | 0.467846 | 3.4:-845731 | MS.gene50824:CDS |
| GAGGAGACCGCCGAAAGCAC+CGG | 0.471355 | 3.4:+844286 | None:intergenic |
| GGATTTCCGCGAAATAAGTC+TGG | 0.473584 | 3.4:+846088 | None:intergenic |
| AGGAAGCAGTAAAGTGAAGA+TGG | 0.475926 | 3.4:-848407 | MS.gene50824:CDS |
| GCCGAAAGCACCGGCAAAAG+AGG | 0.478465 | 3.4:+844295 | None:intergenic |
| CAGGAGGAGAAAAGAGAGGT+TGG | 0.480980 | 3.4:+848277 | None:intergenic |
| GGCCTCAGAGGCTCCTACTC+AGG | 0.481631 | 3.4:-838813 | MS.gene50824:intron |
| AGAGGCTTTGCAATGCTTGA+TGG | 0.482040 | 3.4:+848328 | None:intergenic |
| AAAGCAAGCATCTTGGTCTT+GGG | 0.482070 | 3.4:+845877 | None:intergenic |
| ATCATGTAGTTTACTGAATG+TGG | 0.483578 | 3.4:-846430 | MS.gene50824:CDS |
| GTTCAAACTGAGTGTTTGGC+TGG | 0.486742 | 3.4:+846053 | None:intergenic |
| CCTAAAATCAAATATTCTAA+AGG | 0.489390 | 3.4:+844716 | None:intergenic |
| TGTGCTACCTCTGAGTCCTT+CGG | 0.490928 | 3.4:+838842 | None:intergenic |
| AGGAAATGGCAGTGAGTAGG+AGG | 0.496922 | 3.4:+848301 | None:intergenic |
| AGGAGAAAAGAGAGGTTGGA+AGG | 0.497121 | 3.4:+848281 | None:intergenic |
| ACTTATTTCGCGGAAATCCA+TGG | 0.511640 | 3.4:-846084 | MS.gene50824:CDS |
| GAAGGAGTTTCCAGAAAACT+TGG | 0.511807 | 3.4:-845947 | MS.gene50824:CDS |
| AGCGGCGGACGGTGAGATCA+AGG | 0.514989 | 3.4:+844327 | None:intergenic |
| GGAAGCAGTAAAGTGAAGAT+GGG | 0.524339 | 3.4:-848406 | MS.gene50824:CDS |
| GAGGAGCTTTATTTAGAGGA+TGG | 0.526108 | 3.4:-846366 | MS.gene50824:CDS |
| GGAAGGAAATGGCAGTGAGT+AGG | 0.529323 | 3.4:+848298 | None:intergenic |
| CTACTTCCAGACTTATTTCG+CGG | 0.530504 | 3.4:-846094 | MS.gene50824:CDS |
| CTTCCTCTCTCTTTACTGGA+AGG | 0.534336 | 3.4:+844624 | None:intergenic |
| TACCGCGTGGACAGAATGGC+TGG | 0.535143 | 3.4:-845987 | MS.gene50824:CDS |
| ATAGAAGCCCAGCGAGTCTG+AGG | 0.535981 | 3.4:+838950 | None:intergenic |
| GACACAGTGTCCATATCCAT+TGG | 0.542279 | 3.4:+844690 | None:intergenic |
| GCATATCGGGTAGCTTGCAA+TGG | 0.544452 | 3.4:-846231 | MS.gene50824:CDS |
| GTATGACGTCGATGCCAAAT+GGG | 0.550085 | 3.4:-844357 | MS.gene50824:CDS |
| CCGATATGCAAAATCTCTTG+TGG | 0.552409 | 3.4:+846245 | None:intergenic |
| CCAACTCGTTCCGTTCCGAT+GGG | 0.554127 | 3.4:-844410 | MS.gene50824:CDS |
| CAGTGCCAAAGCAAGCATCT+TGG | 0.554303 | 3.4:+845870 | None:intergenic |
| CCAGTAAAGAGAGAGGAAGC+AGG | 0.556011 | 3.4:-844620 | MS.gene50824:CDS |
| GGAGAAGGTACTGACTGAAA+AGG | 0.557850 | 3.4:+844264 | None:intergenic |
| AAAACATATTGCCGATGTGA+TGG | 0.560080 | 3.4:-844744 | MS.gene50824:CDS |
| AAGACAAATCCCACCTGAGT+AGG | 0.561666 | 3.4:+838800 | None:intergenic |
| ACATTACCGCGTGGACAGAA+TGG | 0.563574 | 3.4:-845991 | MS.gene50824:CDS |
| CCCATCGGAACGGAACGAGT+TGG | 0.566539 | 3.4:+844410 | None:intergenic |
| GCGGACGGTGAGATCAAGGC+AGG | 0.574717 | 3.4:+844331 | None:intergenic |
| ACCTGGGAATATCACTACTT+CGG | 0.584926 | 3.4:+845706 | None:intergenic |
| AGGGAGTCCTCAGACTCGCT+GGG | 0.588615 | 3.4:-838957 | MS.gene50824:CDS |
| TCCGAAGTAGTGATATTCCC+AGG | 0.591003 | 3.4:-845707 | MS.gene50824:intron |
| TAAAGAGAGAGGAAGCAGGC+GGG | 0.593843 | 3.4:-844616 | MS.gene50824:intron |
| CTCAGAGGTAGCACAACCTA+AGG | 0.597908 | 3.4:-838834 | MS.gene50824:CDS |
| AGCAAAGCTTGCACAACCGA+AGG | 0.600829 | 3.4:-838858 | MS.gene50824:CDS |
| TTTCACTTACAGCGCAAAGT+GGG | 0.603080 | 3.4:+845762 | None:intergenic |
| GTAGCACTAGCACTAGCACT+AGG | 0.604565 | 3.4:-848427 | MS.gene50824:CDS |
| TTAATTATGATTAATGAGTG+AGG | 0.606747 | 3.4:-848449 | None:intergenic |
| ACACTGTGTCATAGCTTATG+GGG | 0.614807 | 3.4:-844677 | MS.gene50824:CDS |
| ATTTCACTTACAGCGCAAAG+TGG | 0.618765 | 3.4:+845761 | None:intergenic |
| AAATCAGTTCAAGATACCGC+AGG | 0.618922 | 3.4:-846462 | MS.gene50824:CDS |
| GGAGTAAACGAAGCGGCGGA+CGG | 0.621586 | 3.4:+844316 | None:intergenic |
| GAAGGTACTGACTGAAAAGG+AGG | 0.624142 | 3.4:+844267 | None:intergenic |
| GTTCCTTCCAGTAAAGAGAG+AGG | 0.626530 | 3.4:-844627 | MS.gene50824:CDS |
| GCAGATGAATTGATGACTGA+AGG | 0.634603 | 3.4:-845965 | MS.gene50824:CDS |
| AGCACAACCTAAGGCCTCAG+AGG | 0.635209 | 3.4:-838825 | MS.gene50824:CDS |
| CAGTGAGTAGGAGGATAGAG+AGG | 0.637427 | 3.4:+848310 | None:intergenic |
| CATACAGGAGGAGAAAAGAG+AGG | 0.643705 | 3.4:+848273 | None:intergenic |
| CAAACACTCAGTTTGAACAA+TGG | 0.646343 | 3.4:-846048 | MS.gene50824:CDS |
| GTAAAGAGAGAGGAAGCAGG+CGG | 0.651066 | 3.4:-844617 | MS.gene50824:CDS |
| ATATGCAAAATCTCTTGTGG+AGG | 0.652296 | 3.4:+846248 | None:intergenic |
| CACCTGAGTAGGAGCCTCTG+AGG | 0.654769 | 3.4:+838811 | None:intergenic |
| TCGCGGAAATCCATGGACAA+AGG | 0.657862 | 3.4:-846077 | MS.gene50824:CDS |
| CACTGTGTCATAGCTTATGG+GGG | 0.659991 | 3.4:-844676 | MS.gene50824:CDS |
| AAGAGGAGTAAACGAAGCGG+CGG | 0.665689 | 3.4:+844312 | None:intergenic |
| GAAGCAGTAAAGTGAAGATG+GGG | 0.669526 | 3.4:-848405 | MS.gene50824:CDS |
| TAAAGGTAACTCCATCACAT+CGG | 0.678136 | 3.4:+844733 | None:intergenic |
| ATGTGAATTAGTCAGTGGAG+AGG | 0.695974 | 3.4:-846385 | MS.gene50824:CDS |
| TTGCTGGACATAACCCTGAG+AGG | 0.698077 | 3.4:-846024 | MS.gene50824:CDS |
| TGCACAACCGAAGGACTCAG+AGG | 0.701693 | 3.4:-838849 | MS.gene50824:CDS |
| CAAAAGAGGAGTAAACGAAG+CGG | 0.703591 | 3.4:+844309 | None:intergenic |
| GATGCATGTGAATTAGTCAG+TGG | 0.732474 | 3.4:-846390 | MS.gene50824:CDS |
| TGCCAGCCATTCTGTCCACG+CGG | 0.754261 | 3.4:+845985 | None:intergenic |
| TTGCAAAAGACATTACCGCG+TGG | 0.790610 | 3.4:-846000 | MS.gene50824:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 838249 | 848458 | 838249 | ID=MS.gene50824 |
| chr3.4 | mRNA | 838249 | 848458 | 838249 | ID=MS.gene50824.t1;Parent=MS.gene50824 |
| chr3.4 | exon | 848278 | 848458 | 848278 | ID=MS.gene50824.t1.exon1;Parent=MS.gene50824.t1 |
| chr3.4 | CDS | 848278 | 848458 | 848278 | ID=cds.MS.gene50824.t1;Parent=MS.gene50824.t1 |
| chr3.4 | exon | 846225 | 846519 | 846225 | ID=MS.gene50824.t1.exon2;Parent=MS.gene50824.t1 |
| chr3.4 | CDS | 846225 | 846519 | 846225 | ID=cds.MS.gene50824.t1;Parent=MS.gene50824.t1 |
| chr3.4 | exon | 845855 | 846120 | 845855 | ID=MS.gene50824.t1.exon3;Parent=MS.gene50824.t1 |
| chr3.4 | CDS | 845855 | 846120 | 845855 | ID=cds.MS.gene50824.t1;Parent=MS.gene50824.t1 |
| chr3.4 | exon | 845708 | 845794 | 845708 | ID=MS.gene50824.t1.exon4;Parent=MS.gene50824.t1 |
| chr3.4 | CDS | 845708 | 845794 | 845708 | ID=cds.MS.gene50824.t1;Parent=MS.gene50824.t1 |
| chr3.4 | exon | 844617 | 844770 | 844617 | ID=MS.gene50824.t1.exon5;Parent=MS.gene50824.t1 |
| chr3.4 | CDS | 844617 | 844770 | 844617 | ID=cds.MS.gene50824.t1;Parent=MS.gene50824.t1 |
| chr3.4 | exon | 844279 | 844451 | 844279 | ID=MS.gene50824.t1.exon6;Parent=MS.gene50824.t1 |
| chr3.4 | CDS | 844279 | 844451 | 844279 | ID=cds.MS.gene50824.t1;Parent=MS.gene50824.t1 |
| chr3.4 | exon | 838814 | 838977 | 838814 | ID=MS.gene50824.t1.exon7;Parent=MS.gene50824.t1 |
| chr3.4 | CDS | 838814 | 838977 | 838814 | ID=cds.MS.gene50824.t1;Parent=MS.gene50824.t1 |
| chr3.4 | exon | 838249 | 838269 | 838249 | ID=MS.gene50824.t1.exon8;Parent=MS.gene50824.t1 |
| chr3.4 | CDS | 838249 | 838269 | 838249 | ID=cds.MS.gene50824.t1;Parent=MS.gene50824.t1 |
| Gene Sequence |
| Protein sequence |
