AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50905


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50905.t1 MTR_2g069950 93.939 66 4 0 1 66 268 333 4.23e-36 134
MS.gene50905.t1 MTR_2g069950 59.322 59 21 1 41 96 396 454 5.80e-14 70.1
MS.gene50905.t1 MTR_8g098375 53.086 81 23 3 87 152 201 281 9.27e-16 73.9
MS.gene50905.t1 MTR_5g010150 51.852 81 24 3 87 152 201 281 2.31e-15 72.8
MS.gene50905.t1 MTR_4g415300 50.617 81 25 3 87 152 204 284 6.17e-15 71.6
MS.gene50905.t1 MTR_3g070210 49.383 81 26 2 87 152 204 284 2.08e-14 70.5
MS.gene50905.t1 MTR_5g082070 49.383 81 26 2 87 152 204 284 5.22e-14 69.3
MS.gene50905.t1 MTR_7g101190 37.903 124 51 5 51 151 152 272 3.52e-13 67.0
MS.gene50905.t1 MTR_1g095070 32.540 126 51 4 60 151 81 206 4.12e-13 65.9
MS.gene50905.t1 MTR_4g059390 31.008 129 49 4 60 151 147 272 2.01e-12 64.7
MS.gene50905.t1 MTR_2g094270 38.554 83 30 2 87 151 195 274 2.34e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50905.t1 AT4G23400 38.583 127 44 4 60 152 155 281 5.00e-16 74.7
MS.gene50905.t1 AT1G72250 53.846 65 30 0 2 66 429 493 1.30e-15 75.1
MS.gene50905.t1 AT1G72250 53.846 65 30 0 2 66 440 504 1.30e-15 75.1
MS.gene50905.t1 AT1G72250 53.846 65 30 0 2 66 441 505 1.30e-15 75.1
MS.gene50905.t1 AT4G00430 51.852 81 24 3 87 152 201 281 2.63e-15 72.8
MS.gene50905.t1 AT1G01620 50.617 81 25 3 87 152 128 208 2.87e-15 71.6
MS.gene50905.t1 AT1G01620 50.617 81 25 3 87 152 200 280 5.04e-15 72.0
MS.gene50905.t1 AT3G61430 48.148 81 27 3 87 152 200 280 8.50e-14 68.6
MS.gene50905.t1 AT3G61430 48.148 81 27 3 87 152 200 280 8.50e-14 68.6
MS.gene50905.t1 AT2G45960 49.383 81 26 3 87 152 200 280 8.58e-14 68.6
MS.gene50905.t1 AT2G37170 37.903 124 49 4 52 151 194 313 1.48e-13 68.2
MS.gene50905.t1 AT2G37170 37.903 124 49 4 52 151 151 270 1.58e-13 67.8
MS.gene50905.t1 AT3G53420 36.800 125 51 4 51 151 152 272 4.49e-13 66.6
MS.gene50905.t1 AT3G53420 36.800 125 51 4 51 151 152 272 4.49e-13 66.6
MS.gene50905.t1 AT2G39010 33.333 129 46 4 60 151 146 271 5.95e-13 66.2
MS.gene50905.t1 AT2G37180 37.097 124 50 4 52 151 151 270 1.24e-12 65.5
MS.gene50905.t1 AT1G72250 48.387 62 32 0 5 66 423 484 7.56e-12 63.9
MS.gene50905.t1 AT3G54820 36.885 122 55 5 51 151 151 271 9.16e-12 62.8
MS.gene50905.t1 AT5G60660 37.097 124 52 5 51 151 152 272 1.27e-11 62.4

Find 37 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGCACCTTCAATATATT+TGG 0.109558 3.3:+14372945 None:intergenic
TATTGGATAGATTATGAAAA+TGG 0.171541 3.3:-14373208 MS.gene50905:intron
GATCTAGCTCACAGGTTTAA+AGG 0.197029 3.3:-14371934 MS.gene50905:CDS
TTCTGGGTTGGACCATTTAT+TGG 0.216605 3.3:-14372114 MS.gene50905:CDS
GCAAGAGCAGCTCCAATAAA+TGG 0.282926 3.3:+14372102 None:intergenic
TGAAGCAGATATAAGTGAAT+TGG 0.306119 3.3:-14373142 MS.gene50905:CDS
AGGCTCGAGGAGTAAATTTC+AGG 0.331803 3.3:-14372452 MS.gene50905:CDS
ATCTAGCTCACAGGTTTAAA+GGG 0.353925 3.3:-14371933 MS.gene50905:CDS
CTTTACCAGGGACTTTGCTT+GGG 0.380297 3.3:-14372255 MS.gene50905:CDS
GAAGCAGATATAAGTGAATT+GGG 0.404189 3.3:-14373141 MS.gene50905:CDS
TCTTTACCAGGGACTTTGCT+TGG 0.415464 3.3:-14372256 MS.gene50905:CDS
CAAGAACACATCAAAATCAA+TGG 0.436859 3.3:+14372300 None:intergenic
GGAACACATGAAGCTGTTAG+AGG 0.445329 3.3:-14373187 MS.gene50905:CDS
TTCAGTGCCTTCCATTGTGA+AGG 0.449536 3.3:+14372476 None:intergenic
AGATGGCTCGGATGACAATC+TGG 0.450464 3.3:+14372067 None:intergenic
TGATGTGTTCTTGGTCCACT+TGG 0.459222 3.3:-14372290 MS.gene50905:CDS
GCATATGGACAGACTGGGAC+TGG 0.484273 3.3:-14372504 MS.gene50905:intron
GAGCTGCCATCATCTTTACC+AGG 0.490616 3.3:-14372268 MS.gene50905:CDS
CTAAAGTCCAAATATATTGA+AGG 0.490676 3.3:-14372952 MS.gene50905:CDS
AAGAACACATCAAAATCAAT+GGG 0.494981 3.3:+14372301 None:intergenic
TTGCAGTGGATATTCTGGGT+TGG 0.527082 3.3:-14372126 MS.gene50905:intron
CATTGAATGAAACAACCTCT+TGG 0.529807 3.3:-14371966 MS.gene50905:CDS
AAAGTCCCTGGTAAAGATGA+TGG 0.552855 3.3:+14372262 None:intergenic
AGACTTGATGAGGAGATTGA+AGG 0.561520 3.3:-14373314 None:intergenic
ACATCAAAATCAATGGGAAG+AGG 0.565182 3.3:+14372307 None:intergenic
CAAATATATTGAAGGTGCCA+AGG 0.571305 3.3:-14372944 MS.gene50905:CDS
CAAACACAACAGCAGCAAGA+TGG 0.581074 3.3:+14372050 None:intergenic
GTCATCCCAAGCAAAGTCCC+TGG 0.588043 3.3:+14372250 None:intergenic
ACAACAGCAGCAAGATGGCT+CGG 0.597911 3.3:+14372055 None:intergenic
CACAATGGAAGGCACTGAAG+AGG 0.611605 3.3:-14372472 MS.gene50905:CDS
ATGAGCAAGATCTAGCTCAC+AGG 0.618206 3.3:-14371942 MS.gene50905:CDS
AAGGTGATAGAAAACGTACA+TGG 0.633005 3.3:+14372228 None:intergenic
AGCTGCCATCATCTTTACCA+GGG 0.640815 3.3:-14372267 MS.gene50905:CDS
TAGATCTTGCTCATACCAAG+AGG 0.668311 3.3:+14371951 None:intergenic
AAGATGATGGCAGCTCCAAG+TGG 0.676157 3.3:+14372275 None:intergenic
GAAGGCACTGAAGAGGCTCG+AGG 0.685190 3.3:-14372465 MS.gene50905:CDS
TGGCTCGGATGACAATCTGG+TGG 0.725105 3.3:+14372070 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAAAAATAAGGTAT+TGG - chr3.3:14372352-14372371 MS.gene50905:intron 10.0%
!!! ATGATATGAAATTTTTTAAA+AGG - chr3.3:14371968-14371987 MS.gene50905:CDS 10.0%
!!! TAAATTTGTTCATAAAAATA+AGG + chr3.3:14373025-14373044 None:intergenic 10.0%
!! TTTAAATTTGATGTTATCTT+TGG - chr3.3:14372521-14372540 MS.gene50905:intron 15.0%
!!! TCAAATTTAAAAGTACTTTT+TGG + chr3.3:14372512-14372531 None:intergenic 15.0%
!! ATGTTTGATACAGTATTATA+TGG - chr3.3:14372814-14372833 MS.gene50905:intron 20.0%
!! TATTGGATAGATTATGAAAA+TGG - chr3.3:14372023-14372042 MS.gene50905:CDS 20.0%
!!! AATGTGTTTGTCAATTTTAT+TGG - chr3.3:14372006-14372025 MS.gene50905:CDS 20.0%
!!! TCAAAAAGAGCTTTATAATA+AGG - chr3.3:14372311-14372330 MS.gene50905:CDS 20.0%
! AAGAACACATCAAAATCAAT+GGG + chr3.3:14372933-14372952 None:intergenic 25.0%
! CTAAAGTCCAAATATATTGA+AGG - chr3.3:14372279-14372298 MS.gene50905:CDS 25.0%
!! AATGTATGCATTTTTGCATA+TGG - chr3.3:14372712-14372731 MS.gene50905:intron 25.0%
!! AGAGCTTTATAATAAGGTAT+TGG - chr3.3:14372317-14372336 MS.gene50905:CDS 25.0%
!! ATTCTATCCATCTAAAACTA+AGG + chr3.3:14372695-14372714 None:intergenic 25.0%
!!! AATGTGGTTATTCTTTTTTG+GGG - chr3.3:14372884-14372903 MS.gene50905:intron 25.0%
!!! CAATGTGGTTATTCTTTTTT+GGG - chr3.3:14372883-14372902 MS.gene50905:intron 25.0%
!!! TCAATGTGGTTATTCTTTTT+TGG - chr3.3:14372882-14372901 MS.gene50905:intron 25.0%
!!! TTATTGATGATATTTTGCAG+TGG - chr3.3:14373091-14373110 MS.gene50905:intron 25.0%
AATACTGAAGAGATGAAAGA+AGG - chr3.3:14372419-14372438 MS.gene50905:intron 30.0%
ACTTAAGCATTAATGCCATA+GGG - chr3.3:14372563-14372582 MS.gene50905:intron 30.0%
CAAGAACACATCAAAATCAA+TGG + chr3.3:14372934-14372953 None:intergenic 30.0%
CTTTATAGCTGATATCTTTG+AGG - chr3.3:14372651-14372670 MS.gene50905:intron 30.0%
GAAGCAGATATAAGTGAATT+GGG - chr3.3:14372090-14372109 MS.gene50905:CDS 30.0%
GACTAACTCTAATTTCAATG+TGG - chr3.3:14372868-14372887 MS.gene50905:intron 30.0%
TACTTAAGCATTAATGCCAT+AGG - chr3.3:14372562-14372581 MS.gene50905:intron 30.0%
TGAAGCAGATATAAGTGAAT+TGG - chr3.3:14372089-14372108 MS.gene50905:CDS 30.0%
! ATAAAGCTCTTTTTGAACCT+TGG + chr3.3:14372307-14372326 None:intergenic 30.0%
! ATATTTTGCAGTGGATATTC+TGG - chr3.3:14373100-14373119 MS.gene50905:intron 30.0%
! TATTTTGCAGTGGATATTCT+GGG - chr3.3:14373101-14373120 MS.gene50905:intron 30.0%
! TCATTCACTCAACTTTTGTT+AGG + chr3.3:14371952-14371971 None:intergenic 30.0%
! TCTAAAACTAAGGTTGCAAA+TGG + chr3.3:14372685-14372704 None:intergenic 30.0%
!! TTTTGGGGAATTATAGATCT+TGG - chr3.3:14372899-14372918 MS.gene50905:intron 30.0%
!!! CATTGATTTTGATGTGTTCT+TGG - chr3.3:14372932-14372951 MS.gene50905:CDS 30.0%
!!! TTTGCAACCTTAGTTTTAGA+TGG - chr3.3:14372685-14372704 MS.gene50905:intron 30.0%
AAGGTGATAGAAAACGTACA+TGG + chr3.3:14373006-14373025 None:intergenic 35.0%
AAGTACCTTGTTCAACTTGA+GGG + chr3.3:14372547-14372566 None:intergenic 35.0%
ACATCAAAATCAATGGGAAG+AGG + chr3.3:14372927-14372946 None:intergenic 35.0%
CATTGAATGAAACAACCTCT+TGG - chr3.3:14373265-14373284 MS.gene50905:intron 35.0%
CTTGGCACCTTCAATATATT+TGG + chr3.3:14372289-14372308 None:intergenic 35.0%
TAAGTACCTTGTTCAACTTG+AGG + chr3.3:14372548-14372567 None:intergenic 35.0%
TCGAGCAGAATCAAAATCTA+AGG + chr3.3:14372456-14372475 None:intergenic 35.0%
! ATCAAAATCTAAGGCCATTG+TGG + chr3.3:14372447-14372466 None:intergenic 35.0%
! ATCTAGCTCACAGGTTTAAA+GGG - chr3.3:14373298-14373317 MS.gene50905:intron 35.0%
! ATTTTTGCATATGGACAGAC+TGG - chr3.3:14372721-14372740 MS.gene50905:intron 35.0%
! TTCATCTCTTCAGTATTGAG+AGG + chr3.3:14372416-14372435 None:intergenic 35.0%
! TTTTTGCATATGGACAGACT+GGG - chr3.3:14372722-14372741 MS.gene50905:intron 35.0%
!! AATAAGGTATTGGAGTTGAG+AGG - chr3.3:14372362-14372381 MS.gene50905:intron 35.0%
!! CAAATATATTGAAGGTGCCA+AGG - chr3.3:14372287-14372306 MS.gene50905:CDS 35.0%
!!! CCATAGGGTCTTTTTAACTT+CGG - chr3.3:14372578-14372597 MS.gene50905:intron 35.0%
AAAGTCCCTGGTAAAGATGA+TGG + chr3.3:14372972-14372991 None:intergenic 40.0%
CCGAAGTTAAAAAGACCCTA+TGG + chr3.3:14372581-14372600 None:intergenic 40.0%
GACTGGAAAAACCTTCACAA+TGG - chr3.3:14372744-14372763 MS.gene50905:intron 40.0%
GGAAAAACCTTCACAATGGA+AGG - chr3.3:14372748-14372767 MS.gene50905:intron 40.0%
GGAGTTGAGAGGAAACATAA+GGG - chr3.3:14372373-14372392 MS.gene50905:intron 40.0%
TACACTGTACACATGCGAAA+GGG + chr3.3:14372196-14372215 None:intergenic 40.0%
TAGATCTTGCTCATACCAAG+AGG + chr3.3:14373283-14373302 None:intergenic 40.0%
TGGAGTTGAGAGGAAACATA+AGG - chr3.3:14372372-14372391 MS.gene50905:intron 40.0%
! GATCTAGCTCACAGGTTTAA+AGG - chr3.3:14373297-14373316 MS.gene50905:intron 40.0%
! TTCTGGGTTGGACCATTTAT+TGG - chr3.3:14373117-14373136 MS.gene50905:intron 40.0%
!!! GATTTTGATTCTGCTCGAGA+TGG - chr3.3:14372458-14372477 MS.gene50905:CDS 40.0%
AGCTGCCATCATCTTTACCA+GGG - chr3.3:14372964-14372983 MS.gene50905:CDS 45.0%
AGGCTCGAGGAGTAAATTTC+AGG - chr3.3:14372779-14372798 MS.gene50905:intron 45.0%
CAAACACAACAGCAGCAAGA+TGG + chr3.3:14373184-14373203 None:intergenic 45.0%
CTTTACCAGGGACTTTGCTT+GGG - chr3.3:14372976-14372995 MS.gene50905:CDS 45.0%
GAGCTTACTGGACTGTCAAA+TGG - chr3.3:14372482-14372501 MS.gene50905:CDS 45.0%
GATGAAAGAAGGAGCCACAA+TGG - chr3.3:14372430-14372449 MS.gene50905:CDS 45.0%
GCAAGAGCAGCTCCAATAAA+TGG + chr3.3:14373132-14373151 None:intergenic 45.0%
GGAACACATGAAGCTGTTAG+AGG - chr3.3:14372044-14372063 MS.gene50905:CDS 45.0%
GTACACTGTACACATGCGAA+AGG + chr3.3:14372197-14372216 None:intergenic 45.0%
TCTTTACCAGGGACTTTGCT+TGG - chr3.3:14372975-14372994 MS.gene50905:CDS 45.0%
TGATGTGTTCTTGGTCCACT+TGG - chr3.3:14372941-14372960 MS.gene50905:CDS 45.0%
TTCAGTGCCTTCCATTGTGA+AGG + chr3.3:14372758-14372777 None:intergenic 45.0%
TTGCAGTGGATATTCTGGGT+TGG - chr3.3:14373105-14373124 MS.gene50905:intron 45.0%
! ATGAGCAAGATCTAGCTCAC+AGG - chr3.3:14373289-14373308 MS.gene50905:intron 45.0%
! CAGTATTGAGAGGCCTACAA+CGG + chr3.3:14372406-14372425 None:intergenic 45.0%
! TAAGGGACTTTTGCCGTTGT+AGG - chr3.3:14372390-14372409 MS.gene50905:intron 45.0%
!! TTTGGCCCTCAAGTTGAACA+AGG - chr3.3:14372539-14372558 MS.gene50905:intron 45.0%
!! TTTCAAAAAAATAAAAAATA+AGG - chr3.3:14372346-14372365 MS.gene50905:intron 5.0%
ACAACAGCAGCAAGATGGCT+CGG + chr3.3:14373179-14373198 None:intergenic 50.0%
GAGCTGCCATCATCTTTACC+AGG - chr3.3:14372963-14372982 MS.gene50905:CDS 50.0%
! AAGATGATGGCAGCTCCAAG+TGG + chr3.3:14372959-14372978 None:intergenic 50.0%
! AGATGGCTCGGATGACAATC+TGG + chr3.3:14373167-14373186 None:intergenic 50.0%
!! CACAATGGAAGGCACTGAAG+AGG - chr3.3:14372759-14372778 MS.gene50905:intron 50.0%
GCATATGGACAGACTGGGAC+TGG - chr3.3:14372727-14372746 MS.gene50905:intron 55.0%
GCTCGAGATGGTGAGCTTAC+TGG - chr3.3:14372470-14372489 MS.gene50905:CDS 55.0%
GTCATCCCAAGCAAAGTCCC+TGG + chr3.3:14372984-14373003 None:intergenic 55.0%
! TGGCTCGGATGACAATCTGG+TGG + chr3.3:14373164-14373183 None:intergenic 55.0%
!! GAAGGCACTGAAGAGGCTCG+AGG - chr3.3:14372766-14372785 MS.gene50905:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 14371924 14373329 14371924 ID=MS.gene50905
chr3.3 mRNA 14371924 14373329 14371924 ID=MS.gene50905.t1;Parent=MS.gene50905
chr3.3 exon 14373315 14373329 14373315 ID=MS.gene50905.t1.exon1;Parent=MS.gene50905.t1
chr3.3 CDS 14373315 14373329 14373315 ID=cds.MS.gene50905.t1;Parent=MS.gene50905.t1
chr3.3 exon 14373121 14373220 14373121 ID=MS.gene50905.t1.exon2;Parent=MS.gene50905.t1
chr3.3 CDS 14373121 14373220 14373121 ID=cds.MS.gene50905.t1;Parent=MS.gene50905.t1
chr3.3 exon 14372921 14373003 14372921 ID=MS.gene50905.t1.exon3;Parent=MS.gene50905.t1
chr3.3 CDS 14372921 14373003 14372921 ID=cds.MS.gene50905.t1;Parent=MS.gene50905.t1
chr3.3 exon 14372428 14372514 14372428 ID=MS.gene50905.t1.exon4;Parent=MS.gene50905.t1
chr3.3 CDS 14372428 14372514 14372428 ID=cds.MS.gene50905.t1;Parent=MS.gene50905.t1
chr3.3 exon 14372243 14372338 14372243 ID=MS.gene50905.t1.exon5;Parent=MS.gene50905.t1
chr3.3 CDS 14372243 14372338 14372243 ID=cds.MS.gene50905.t1;Parent=MS.gene50905.t1
chr3.3 exon 14371924 14372142 14371924 ID=MS.gene50905.t1.exon6;Parent=MS.gene50905.t1
chr3.3 CDS 14371924 14372142 14371924 ID=cds.MS.gene50905.t1;Parent=MS.gene50905.t1
Gene Sequence

>MS.gene50905

ATGAGGAGATTGAAGGTACTGATATCCTAACAAAAGTTGAGTGAATGATATGAAATTTTTTAAAAGGTAGAGTTATGTTTGTAATGTGTTTGTCAATTTTATTGGATAGATTATGAAAATGGAACACATGAAGCTGTTAGAGGAAGCAGAAGCATACAAGAAATATGAAGCAGATATAAGTGAATTGGGATTCATGATCAAATCTAAAAGTAAAGTAGACAATGTTGCATTAGAATCTCTATCTTGTGCGTGTTAATGTTATTAGTTTTCCCTTTCGCATGTGTACAGTGTACTTAACACTTTATTTTATTTCGTTTTTTTTATAGTGAATGAACAAATAGAGTTGCATGAAGATCTAAAGTCCAAATATATTGAAGGTGCCAAGGTTCAAAAAGAGCTTTATAATAAGGTATTGGAGTTTCTTTCAAAAAAATAAAAAATAAGGTATTGGAGTTGAGAGGAAACATAAGGGACTTTTGCCGTTGTAGGCCTCTCAATACTGAAGAGATGAAAGAAGGAGCCACAATGGCCTTAGATTTTGATTCTGCTCGAGATGGTGAGCTTACTGGACTGTCAAATGGATCTCCAAAAAGTACTTTTAAATTTGATGTTATCTTTGGCCCTCAAGTTGAACAAGGTACTTAAGCATTAATGCCATAGGGTCTTTTTAACTTCGGTTCTGAATTGAAGATAGATGCTTATTTTGACAATTTTTTTTATTGTTTTTCTTTATAGCTGATATCTTTGAGGACACTGTACCATTTGCAACCTTAGTTTTAGATGGATAGAATGTATGCATTTTTGCATATGGACAGACTGGGACTGGAAAAACCTTCACAATGGAAGGCACTGAAGAGGCTCGAGGAGTAAATTTCAGGACTCTTAAGAAAATGTTTGATACAGTATTATATGGTTTTAATTTGTGAATTAATTTCTCAATGATTGACTAACTCTAATTTCAATGTGGTTATTCTTTTTTGGGGAATTATAGATCTTGGTTCCTCTTCCCATTGATTTTGATGTGTTCTTGGTCCACTTGGAGCTGCCATCATCTTTACCAGGGACTTTGCTTGGGATGACCATGTACGTTTTCTATCACCTTATTTTTATGAACAAATTTAGACTCTAATTTTTGTTCTTTAAATTAAATAATTATTTAATATTGTATTATTGATGATATTTTGCAGTGGATATTCTGGGTTGGACCATTTATTGGAGCTGCTCTTGCTGCTGTGTACCACCAGATTGTCATCCGAGCCATCTTGCTGCTGTTGTGTTTGTTTACTTCTATTTGCTTAAGTTGTTGTTATTGTTTTTCTTGCTCAACTGTTATGAATGAAACATTGAATGAAACAACCTCTTGGTATGAGCAAGATCTAGCTCACAGGTTTAAAGGGTTTCTATGA

Protein sequence

>MS.gene50905.t1

MRRLKIMKMEHMKLLEEAEAYKKYEADISELGFMIKSKMNEQIELHEDLKSKYIEGAKVQKELYNKTGTGKTFTMEGTEEARGVNFRTLKKMFDTILVPLPIDFDVFLVHLELPSSLPGTLLGMTMYWIFWVGPFIGAALAAVYHQIVIRAILLLLCLFTSICLSCCYCFSCSTVMNETLNETTSWYEQDLAHRFKGFL