Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50973.t1 | XP_003599668.2 | 72.6 | 310 | 32 | 1 | 1 | 257 | 2 | 311 | 2.20E-120 | 441.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50973.t1 | P81392 | 40.6 | 308 | 112 | 6 | 1 | 238 | 1 | 307 | 5.0e-49 | 196.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50973.t1 | G7IZP9 | 72.6 | 310 | 32 | 1 | 1 | 257 | 2 | 311 | 1.6e-120 | 441.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene50973.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057754 | MS.gene50973 | 0.808152 | 3.56E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50973.t1 | MTR_3g039990 | 77.097 | 310 | 18 | 1 | 1 | 257 | 2 | 311 | 1.63e-171 | 475 |
| MS.gene50973.t1 | MTR_0140s0030 | 41.210 | 347 | 100 | 9 | 1 | 257 | 2 | 334 | 2.34e-70 | 219 |
| MS.gene50973.t1 | MTR_0489s0020 | 41.210 | 347 | 100 | 9 | 1 | 257 | 2 | 334 | 2.34e-70 | 219 |
| MS.gene50973.t1 | MTR_7g010210 | 41.358 | 324 | 102 | 11 | 1 | 245 | 1 | 315 | 1.03e-58 | 189 |
| MS.gene50973.t1 | MTR_6g012180 | 38.365 | 318 | 105 | 7 | 1 | 239 | 1 | 306 | 4.88e-52 | 172 |
| MS.gene50973.t1 | MTR_7g010210 | 46.296 | 216 | 40 | 5 | 1 | 141 | 1 | 215 | 8.86e-49 | 162 |
| MS.gene50973.t1 | MTR_7g087130 | 30.748 | 361 | 111 | 10 | 1 | 248 | 1 | 335 | 8.25e-32 | 119 |
| MS.gene50973.t1 | MTR_7g011170 | 33.133 | 166 | 46 | 4 | 1 | 101 | 1 | 166 | 1.41e-20 | 89.0 |
| MS.gene50973.t1 | MTR_1g076150 | 51.220 | 82 | 35 | 2 | 1 | 80 | 1 | 79 | 1.76e-18 | 82.4 |
| MS.gene50973.t1 | MTR_6g012690 | 39.806 | 103 | 55 | 3 | 1 | 100 | 1 | 99 | 7.00e-17 | 79.3 |
| MS.gene50973.t1 | MTR_2g089620 | 44.706 | 85 | 41 | 2 | 1 | 83 | 1 | 81 | 8.18e-17 | 79.0 |
| MS.gene50973.t1 | MTR_4g121460 | 50.649 | 77 | 36 | 1 | 1 | 75 | 1 | 77 | 8.20e-17 | 79.0 |
| MS.gene50973.t1 | MTR_1g043050 | 46.753 | 77 | 39 | 1 | 1 | 75 | 1 | 77 | 4.77e-16 | 75.9 |
| MS.gene50973.t1 | MTR_1g043080 | 47.368 | 76 | 38 | 1 | 1 | 74 | 1 | 76 | 1.10e-15 | 74.7 |
| MS.gene50973.t1 | MTR_4g478180 | 46.269 | 67 | 34 | 1 | 1 | 65 | 2 | 68 | 4.53e-15 | 74.3 |
| MS.gene50973.t1 | MTR_7g115650 | 40.000 | 85 | 45 | 2 | 1 | 83 | 1 | 81 | 8.71e-15 | 71.2 |
| MS.gene50973.t1 | MTR_4g082290 | 41.379 | 87 | 45 | 2 | 1 | 85 | 1 | 83 | 1.32e-14 | 73.2 |
| MS.gene50973.t1 | MTR_3g074520 | 42.683 | 82 | 41 | 2 | 1 | 80 | 1 | 78 | 2.19e-14 | 72.0 |
| MS.gene50973.t1 | MTR_8g027345 | 33.065 | 124 | 30 | 2 | 1 | 71 | 1 | 124 | 2.21e-14 | 71.6 |
| MS.gene50973.t1 | MTR_7g117730 | 40.260 | 77 | 44 | 1 | 1 | 75 | 1 | 77 | 2.32e-14 | 71.2 |
| MS.gene50973.t1 | MTR_1g100653 | 40.217 | 92 | 49 | 2 | 1 | 90 | 1 | 88 | 6.94e-14 | 70.1 |
| MS.gene50973.t1 | MTR_2g011660 | 48.529 | 68 | 32 | 2 | 1 | 65 | 1 | 68 | 1.02e-13 | 70.1 |
| MS.gene50973.t1 | MTR_7g096930 | 37.079 | 89 | 54 | 1 | 1 | 87 | 1 | 89 | 1.41e-13 | 69.3 |
| MS.gene50973.t1 | MTR_2g034790 | 32.353 | 136 | 77 | 3 | 1 | 121 | 1 | 136 | 1.43e-13 | 69.7 |
| MS.gene50973.t1 | MTR_4g082230 | 33.613 | 119 | 26 | 2 | 1 | 66 | 1 | 119 | 1.90e-13 | 69.7 |
| MS.gene50973.t1 | MTR_4g091490 | 35.000 | 120 | 24 | 3 | 1 | 66 | 1 | 120 | 3.21e-13 | 68.9 |
| MS.gene50973.t1 | MTR_7g109320 | 46.377 | 69 | 35 | 1 | 1 | 67 | 1 | 69 | 3.99e-13 | 68.6 |
| MS.gene50973.t1 | MTR_5g007370 | 41.463 | 82 | 42 | 2 | 1 | 80 | 1 | 78 | 4.07e-13 | 68.2 |
| MS.gene50973.t1 | MTR_4g073420 | 44.156 | 77 | 41 | 1 | 1 | 75 | 1 | 77 | 4.24e-13 | 67.8 |
| MS.gene50973.t1 | MTR_8g031360 | 29.221 | 154 | 92 | 5 | 1 | 137 | 1 | 154 | 8.86e-13 | 67.0 |
| MS.gene50973.t1 | MTR_5g029840 | 36.264 | 91 | 56 | 1 | 1 | 89 | 1 | 91 | 9.16e-13 | 67.4 |
| MS.gene50973.t1 | MTR_7g076740 | 47.059 | 68 | 33 | 2 | 1 | 65 | 1 | 68 | 1.31e-12 | 66.6 |
| MS.gene50973.t1 | MTR_3g103570 | 44.872 | 78 | 40 | 2 | 1 | 75 | 1 | 78 | 1.39e-12 | 67.0 |
| MS.gene50973.t1 | MTR_6g009430 | 40.260 | 77 | 44 | 1 | 1 | 75 | 1 | 77 | 1.39e-12 | 67.0 |
| MS.gene50973.t1 | MTR_3g461490 | 40.217 | 92 | 49 | 2 | 1 | 90 | 1 | 88 | 1.94e-12 | 66.2 |
| MS.gene50973.t1 | MTR_7g102110 | 31.707 | 123 | 30 | 2 | 1 | 69 | 1 | 123 | 2.43e-12 | 65.5 |
| MS.gene50973.t1 | MTR_1g017140 | 44.578 | 83 | 39 | 3 | 1 | 80 | 1 | 79 | 6.15e-12 | 65.1 |
| MS.gene50973.t1 | MTR_4g097570 | 43.373 | 83 | 40 | 3 | 1 | 80 | 1 | 79 | 6.68e-12 | 64.7 |
| MS.gene50973.t1 | MTR_4g097570 | 42.857 | 84 | 40 | 3 | 1 | 80 | 1 | 80 | 7.54e-12 | 64.3 |
| MS.gene50973.t1 | MTR_4g102380 | 44.776 | 67 | 35 | 1 | 1 | 65 | 1 | 67 | 8.08e-12 | 63.9 |
| MS.gene50973.t1 | MTR_2g067420 | 39.189 | 74 | 43 | 1 | 3 | 74 | 5 | 78 | 1.14e-11 | 64.3 |
| MS.gene50973.t1 | MTR_2g095520 | 36.667 | 90 | 54 | 2 | 1 | 87 | 1 | 90 | 1.34e-11 | 63.9 |
| MS.gene50973.t1 | MTR_4g125520 | 37.662 | 77 | 46 | 1 | 1 | 75 | 1 | 77 | 1.68e-11 | 63.5 |
| MS.gene50973.t1 | MTR_8g095390 | 36.170 | 94 | 58 | 1 | 1 | 92 | 1 | 94 | 2.77e-11 | 62.8 |
| MS.gene50973.t1 | MTR_3g097450 | 38.202 | 89 | 53 | 1 | 1 | 87 | 1 | 89 | 2.96e-11 | 62.8 |
| MS.gene50973.t1 | MTR_5g078140 | 38.043 | 92 | 51 | 2 | 1 | 90 | 1 | 88 | 3.21e-11 | 62.8 |
| MS.gene50973.t1 | MTR_3g065440 | 38.043 | 92 | 51 | 2 | 1 | 90 | 1 | 88 | 3.28e-11 | 62.8 |
| MS.gene50973.t1 | MTR_4g057635 | 35.556 | 90 | 55 | 2 | 1 | 87 | 1 | 90 | 5.99e-11 | 62.0 |
| MS.gene50973.t1 | MTR_5g014990 | 35.556 | 90 | 55 | 2 | 1 | 87 | 1 | 90 | 6.18e-11 | 62.0 |
| MS.gene50973.t1 | MTR_6g090405 | 37.778 | 90 | 53 | 2 | 1 | 87 | 1 | 90 | 7.25e-11 | 61.2 |
| MS.gene50973.t1 | MTR_4g128670 | 41.026 | 78 | 43 | 2 | 1 | 75 | 1 | 78 | 9.83e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50973.t1 | AT1G74650 | 37.752 | 347 | 101 | 9 | 1 | 251 | 1 | 328 | 1.10e-55 | 182 |
| MS.gene50973.t1 | AT3G47600 | 38.507 | 335 | 89 | 9 | 1 | 237 | 1 | 316 | 1.51e-53 | 176 |
| MS.gene50973.t1 | AT5G62470 | 37.640 | 356 | 87 | 11 | 1 | 239 | 1 | 338 | 5.95e-53 | 175 |
| MS.gene50973.t1 | AT5G62470 | 37.535 | 357 | 87 | 11 | 1 | 239 | 1 | 339 | 8.24e-53 | 175 |
| MS.gene50973.t1 | AT3G28910 | 34.513 | 339 | 107 | 9 | 1 | 246 | 1 | 317 | 1.73e-46 | 158 |
| MS.gene50973.t1 | AT1G08810 | 40.099 | 202 | 38 | 4 | 1 | 148 | 1 | 173 | 1.52e-35 | 128 |
| MS.gene50973.t1 | AT1G74650 | 34.686 | 271 | 114 | 9 | 25 | 251 | 1 | 252 | 2.21e-33 | 122 |
| MS.gene50973.t1 | AT1G74650 | 35.907 | 259 | 103 | 9 | 37 | 251 | 8 | 247 | 6.64e-33 | 120 |
| MS.gene50973.t1 | AT3G47600 | 34.799 | 273 | 112 | 10 | 11 | 237 | 58 | 310 | 4.71e-31 | 117 |
| MS.gene50973.t1 | AT3G28910 | 30.682 | 264 | 118 | 10 | 25 | 246 | 1 | 241 | 1.01e-22 | 94.0 |
| MS.gene50973.t1 | AT5G15310 | 45.882 | 85 | 40 | 2 | 1 | 83 | 1 | 81 | 1.47e-17 | 79.7 |
| MS.gene50973.t1 | AT5G15310 | 45.882 | 85 | 40 | 2 | 1 | 83 | 1 | 81 | 4.11e-17 | 79.7 |
| MS.gene50973.t1 | AT5G15310 | 45.882 | 85 | 40 | 2 | 1 | 83 | 1 | 81 | 4.52e-17 | 79.7 |
| MS.gene50973.t1 | AT5G49330 | 49.351 | 77 | 37 | 1 | 1 | 75 | 1 | 77 | 5.39e-17 | 79.7 |
| MS.gene50973.t1 | AT3G61250 | 31.544 | 149 | 85 | 4 | 1 | 132 | 1 | 149 | 1.03e-16 | 78.2 |
| MS.gene50973.t1 | AT3G01140 | 49.254 | 67 | 32 | 1 | 1 | 65 | 1 | 67 | 4.24e-16 | 77.0 |
| MS.gene50973.t1 | AT4G21440 | 50.746 | 67 | 31 | 1 | 1 | 65 | 1 | 67 | 4.48e-16 | 77.0 |
| MS.gene50973.t1 | AT3G23250 | 47.887 | 71 | 35 | 1 | 1 | 69 | 1 | 71 | 7.31e-16 | 75.9 |
| MS.gene50973.t1 | AT1G34670 | 33.613 | 119 | 26 | 1 | 1 | 66 | 1 | 119 | 8.37e-16 | 76.3 |
| MS.gene50973.t1 | AT3G01140 | 43.529 | 85 | 42 | 2 | 1 | 83 | 44 | 124 | 1.29e-15 | 75.9 |
| MS.gene50973.t1 | AT4G28110 | 49.254 | 67 | 32 | 1 | 1 | 65 | 1 | 67 | 1.62e-15 | 74.7 |
| MS.gene50973.t1 | AT2G31180 | 47.887 | 71 | 35 | 1 | 1 | 69 | 1 | 71 | 1.63e-15 | 74.3 |
| MS.gene50973.t1 | AT2G47460 | 45.455 | 77 | 40 | 1 | 1 | 75 | 1 | 77 | 7.02e-15 | 73.9 |
| MS.gene50973.t1 | AT1G06180 | 47.887 | 71 | 35 | 1 | 1 | 69 | 1 | 71 | 9.54e-15 | 72.0 |
| MS.gene50973.t1 | AT1G79180 | 45.946 | 74 | 38 | 1 | 3 | 74 | 5 | 78 | 2.23e-14 | 71.6 |
| MS.gene50973.t1 | AT1G22640 | 44.156 | 77 | 41 | 1 | 1 | 75 | 1 | 77 | 3.62e-14 | 70.5 |
| MS.gene50973.t1 | AT3G02940 | 37.079 | 89 | 54 | 1 | 1 | 87 | 1 | 89 | 5.06e-14 | 70.9 |
| MS.gene50973.t1 | AT5G16770 | 28.182 | 220 | 89 | 4 | 1 | 167 | 1 | 204 | 6.15e-14 | 70.9 |
| MS.gene50973.t1 | AT5G16770 | 28.182 | 220 | 89 | 4 | 1 | 167 | 1 | 204 | 6.15e-14 | 70.9 |
| MS.gene50973.t1 | AT3G02940 | 37.079 | 89 | 54 | 1 | 1 | 87 | 1 | 89 | 6.34e-14 | 70.9 |
| MS.gene50973.t1 | AT3G02940 | 37.079 | 89 | 54 | 1 | 1 | 87 | 1 | 89 | 6.34e-14 | 70.9 |
| MS.gene50973.t1 | AT3G13540 | 29.834 | 181 | 104 | 5 | 5 | 164 | 16 | 194 | 6.89e-14 | 69.7 |
| MS.gene50973.t1 | AT3G62610 | 42.857 | 77 | 42 | 1 | 1 | 75 | 1 | 77 | 8.47e-14 | 70.5 |
| MS.gene50973.t1 | AT4G05100 | 36.667 | 120 | 22 | 3 | 1 | 66 | 1 | 120 | 9.16e-14 | 70.1 |
| MS.gene50973.t1 | AT5G16770 | 28.182 | 220 | 89 | 4 | 1 | 167 | 1 | 204 | 9.23e-14 | 70.5 |
| MS.gene50973.t1 | AT5G16770 | 28.182 | 220 | 89 | 4 | 1 | 167 | 1 | 204 | 9.23e-14 | 70.5 |
| MS.gene50973.t1 | AT2G16720 | 46.753 | 77 | 39 | 1 | 1 | 75 | 1 | 77 | 1.20e-13 | 69.3 |
| MS.gene50973.t1 | AT5G60890 | 43.284 | 67 | 36 | 1 | 1 | 65 | 1 | 67 | 1.21e-13 | 69.7 |
| MS.gene50973.t1 | AT4G09460 | 45.455 | 77 | 40 | 1 | 1 | 75 | 1 | 77 | 1.75e-13 | 68.6 |
| MS.gene50973.t1 | AT1G74080 | 41.463 | 82 | 42 | 2 | 1 | 80 | 1 | 78 | 2.05e-13 | 69.3 |
| MS.gene50973.t1 | AT1G18570 | 43.373 | 83 | 40 | 3 | 1 | 80 | 1 | 79 | 2.71e-13 | 68.9 |
| MS.gene50973.t1 | AT1G56160 | 40.000 | 80 | 42 | 2 | 3 | 80 | 5 | 80 | 5.74e-13 | 67.8 |
| MS.gene50973.t1 | AT4G38620 | 44.156 | 77 | 41 | 1 | 1 | 75 | 1 | 77 | 7.03e-13 | 67.4 |
| MS.gene50973.t1 | AT4G37780 | 43.590 | 78 | 41 | 2 | 1 | 75 | 1 | 78 | 1.01e-12 | 67.0 |
| MS.gene50973.t1 | AT1G35515 | 42.857 | 77 | 42 | 1 | 1 | 75 | 1 | 77 | 1.08e-12 | 65.9 |
| MS.gene50973.t1 | AT5G61420 | 29.333 | 150 | 84 | 5 | 1 | 128 | 1 | 150 | 1.24e-12 | 67.4 |
| MS.gene50973.t1 | AT4G34990 | 42.857 | 77 | 42 | 1 | 1 | 75 | 1 | 77 | 1.33e-12 | 66.6 |
| MS.gene50973.t1 | AT5G56110 | 45.122 | 82 | 39 | 2 | 1 | 80 | 1 | 78 | 2.14e-12 | 66.2 |
| MS.gene50973.t1 | AT5G07690 | 43.284 | 67 | 36 | 1 | 1 | 65 | 1 | 67 | 2.89e-12 | 65.9 |
| MS.gene50973.t1 | AT1G79180 | 64.103 | 39 | 14 | 0 | 3 | 41 | 5 | 43 | 4.13e-12 | 65.5 |
| MS.gene50973.t1 | AT4G17785 | 42.169 | 83 | 41 | 3 | 1 | 80 | 1 | 79 | 2.76e-11 | 63.2 |
| MS.gene50973.t1 | AT1G66230 | 43.284 | 67 | 36 | 1 | 1 | 65 | 1 | 67 | 2.80e-11 | 62.8 |
| MS.gene50973.t1 | AT4G17785 | 42.169 | 83 | 41 | 3 | 1 | 80 | 1 | 79 | 3.14e-11 | 63.2 |
| MS.gene50973.t1 | AT3G28470 | 43.284 | 67 | 36 | 1 | 1 | 65 | 1 | 67 | 3.32e-11 | 62.8 |
| MS.gene50973.t1 | AT5G54230 | 44.595 | 74 | 35 | 2 | 12 | 83 | 12 | 81 | 4.71e-11 | 62.4 |
| MS.gene50973.t1 | AT4G22680 | 34.066 | 91 | 58 | 1 | 1 | 89 | 1 | 91 | 4.88e-11 | 62.0 |
| MS.gene50973.t1 | AT5G14340 | 38.043 | 92 | 51 | 2 | 1 | 90 | 13 | 100 | 5.56e-11 | 61.6 |
| MS.gene50973.t1 | AT3G49690 | 43.373 | 83 | 40 | 3 | 1 | 80 | 1 | 79 | 5.92e-11 | 62.0 |
| MS.gene50973.t1 | AT1G16490 | 36.145 | 83 | 47 | 2 | 3 | 83 | 5 | 83 | 6.07e-11 | 61.6 |
| MS.gene50973.t1 | AT5G55020 | 39.024 | 82 | 48 | 1 | 10 | 89 | 24 | 105 | 6.61e-11 | 62.4 |
Find 45 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAGAACAATCAGAATTTA+AGG | 0.139704 | 3.2:+27609191 | None:intergenic |
| TCAAGTCCCTCTCATGTTTA+TGG | 0.172493 | 3.2:-27609088 | MS.gene50973:CDS |
| AAATTCAAGAAACTCCTTTA+TGG | 0.223423 | 3.2:-27609307 | MS.gene50973:CDS |
| CCACCTTGCTGTGAGAAAAT+TGG | 0.226124 | 3.2:-27610797 | MS.gene50973:CDS |
| TCCAGAGGAGGATATAATTT+TGG | 0.242453 | 3.2:-27610753 | MS.gene50973:CDS |
| AACTCCTTTATGGTAGTTAC+AGG | 0.269651 | 3.2:-27609297 | MS.gene50973:CDS |
| AACTCTATTCCTCAAGTAAA+AGG | 0.271006 | 3.2:-27609519 | MS.gene50973:CDS |
| CACCTTGCTGTGAGAAAATT+GGG | 0.291064 | 3.2:-27610796 | MS.gene50973:CDS |
| AAGAACATGGACCTGGAAAT+TGG | 0.305090 | 3.2:-27610718 | MS.gene50973:CDS |
| CTCTCATGTTTATGGAGAAT+TGG | 0.317349 | 3.2:-27609080 | MS.gene50973:CDS |
| GGGACTTGAGTTTCCTGGTT+TGG | 0.318854 | 3.2:+27609102 | None:intergenic |
| TGAGAGGGACTTGAGTTTCC+TGG | 0.343799 | 3.2:+27609097 | None:intergenic |
| GGAACTGATCTCCAATTTCC+AGG | 0.410901 | 3.2:+27610707 | None:intergenic |
| TCCTCAAGTAAAAGGTCAAT+GGG | 0.432698 | 3.2:-27609511 | MS.gene50973:CDS |
| TTCCTCAAGTAAAAGGTCAA+TGG | 0.439466 | 3.2:-27609512 | MS.gene50973:CDS |
| AGATCCTGTAACTACCATAA+AGG | 0.458848 | 3.2:+27609293 | None:intergenic |
| CTTAAGAAGATCAATCAAAC+AGG | 0.475240 | 3.2:-27609564 | MS.gene50973:CDS |
| GAGAAAATTGGGGTTAAGAA+AGG | 0.485693 | 3.2:-27610785 | MS.gene50973:CDS |
| TGAGCAGCCTCTTCATCAAA+AGG | 0.486075 | 3.2:+27609057 | None:intergenic |
| AGATCAGTTCCCACCACTAC+AGG | 0.486392 | 3.2:-27610695 | MS.gene50973:intron |
| ACCAAAATTATATCCTCCTC+TGG | 0.493239 | 3.2:+27610752 | None:intergenic |
| TAGCTAGATTACTCGAAAAG+TGG | 0.506269 | 3.2:-27609362 | MS.gene50973:CDS |
| TTTCAGTGTTTGATGCATAT+GGG | 0.509030 | 3.2:+27609388 | None:intergenic |
| AGATCTAATGAACGTTTCAA+TGG | 0.514885 | 3.2:-27609025 | MS.gene50973:CDS |
| ACTATTGGAATACACATTTG+AGG | 0.515179 | 3.2:-27609593 | MS.gene50973:CDS |
| TCAATGGAGGAAAATACAGC+AGG | 0.530896 | 3.2:-27609009 | MS.gene50973:CDS |
| TGATTGTTCTCAATCTGTGT+CGG | 0.532600 | 3.2:-27609181 | MS.gene50973:CDS |
| ACTTCAAACAGATATCCATA+TGG | 0.546568 | 3.2:-27609481 | MS.gene50973:CDS |
| ATATCCTCCTCTGGAGTCCA+AGG | 0.551517 | 3.2:+27610761 | None:intergenic |
| TACATTCAAGAACATGGACC+TGG | 0.568304 | 3.2:-27610725 | MS.gene50973:CDS |
| TCAACAAAGAATCAAATACT+TGG | 0.570464 | 3.2:+27609223 | None:intergenic |
| GTATCATACATTCAAGAACA+TGG | 0.579079 | 3.2:-27610731 | MS.gene50973:CDS |
| TCTAATGAACGTTTCAATGG+AGG | 0.584068 | 3.2:-27609022 | MS.gene50973:CDS |
| TTGGGGTTAAGAAAGGACCT+TGG | 0.585675 | 3.2:-27610778 | MS.gene50973:CDS |
| ACAGGATCTAGTTCTAGTGA+AGG | 0.595194 | 3.2:-27609279 | MS.gene50973:CDS |
| TTCAGTGTTTGATGCATATG+GGG | 0.607862 | 3.2:+27609389 | None:intergenic |
| ATATGAAAGTAAGCCAAACC+AGG | 0.614219 | 3.2:-27609115 | MS.gene50973:CDS |
| GGATCTAGTTCTAGTGAAGG+AGG | 0.624680 | 3.2:-27609276 | MS.gene50973:CDS |
| AATTCTCCATAAACATGAGA+GGG | 0.633240 | 3.2:+27609082 | None:intergenic |
| CAATTCTCCATAAACATGAG+AGG | 0.644962 | 3.2:+27609081 | None:intergenic |
| CAATGGAGGAAAATACAGCA+GGG | 0.659954 | 3.2:-27609008 | MS.gene50973:CDS |
| GAAAGGACCTTGGACTCCAG+AGG | 0.661042 | 3.2:-27610768 | MS.gene50973:CDS |
| GGCAAAACAAGCTTTGTGTG+AGG | 0.680287 | 3.2:-27609460 | MS.gene50973:CDS |
| AGGACCTTGGACTCCAGAGG+AGG | 0.681544 | 3.2:-27610765 | MS.gene50973:CDS |
| ACCTTGCTGTGAGAAAATTG+GGG | 0.707642 | 3.2:-27610795 | MS.gene50973:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTAAAGCAAAAAAAAAA+TGG | + | chr3.2:27610113-27610132 | None:intergenic | 10.0% |
| !!! | ATCTCTTATTTTTTTTTTTA+AGG | - | chr3.2:27609620-27609639 | MS.gene50973:CDS | 10.0% |
| !! | AAAAAATATTGCAAAAAATC+TGG | + | chr3.2:27609681-27609700 | None:intergenic | 15.0% |
| !! | TTAATTCATGTGTAAAATTT+GGG | + | chr3.2:27609812-27609831 | None:intergenic | 15.0% |
| !!! | ATAATTCATTTTCAACAATT+TGG | + | chr3.2:27609870-27609889 | None:intergenic | 15.0% |
| !!! | ATTCTAAACTTTAACAATTT+TGG | - | chr3.2:27609701-27609720 | MS.gene50973:intron | 15.0% |
| !!! | TTTATTGATTTTTCTTTTTC+AGG | - | chr3.2:27609453-27609472 | MS.gene50973:CDS | 15.0% |
| !!! | TTTTTGTTTATAAGACAAAT+TGG | + | chr3.2:27609774-27609793 | None:intergenic | 15.0% |
| !! | AAAAAATAAGAGATGAGATT+AGG | + | chr3.2:27609614-27609633 | None:intergenic | 20.0% |
| !! | ACATATGAATTTATGTAGAT+GGG | - | chr3.2:27610141-27610160 | MS.gene50973:intron | 20.0% |
| !! | CTTAATTCATGTGTAAAATT+TGG | + | chr3.2:27609813-27609832 | None:intergenic | 20.0% |
| !! | TAAAGCAAAAAAAAAATGGT+GGG | + | chr3.2:27610109-27610128 | None:intergenic | 20.0% |
| !! | TTAAAGCAAAAAAAAAATGG+TGG | + | chr3.2:27610110-27610129 | None:intergenic | 20.0% |
| !!! | ATTTTACACATGAATTAAGT+CGG | - | chr3.2:27609814-27609833 | MS.gene50973:intron | 20.0% |
| !!! | GTTTATGTTTTTGATTTTGT+AGG | - | chr3.2:27609427-27609446 | MS.gene50973:CDS | 20.0% |
| ! | AAAGCAAAAAAAAAATGGTG+GGG | + | chr3.2:27610108-27610127 | None:intergenic | 25.0% |
| ! | AAATTCAAGAAACTCCTTTA+TGG | - | chr3.2:27610498-27610517 | MS.gene50973:intron | 25.0% |
| ! | ACAATGACATCAAAAACTAT+TGG | - | chr3.2:27610197-27610216 | MS.gene50973:intron | 25.0% |
| ! | GACATATGAATTTATGTAGA+TGG | - | chr3.2:27610140-27610159 | MS.gene50973:intron | 25.0% |
| ! | TCAACAAAGAATCAAATACT+TGG | + | chr3.2:27610585-27610604 | None:intergenic | 25.0% |
| ! | TCGTCTATGAAAATGAAATT+TGG | - | chr3.2:27609975-27609994 | MS.gene50973:intron | 25.0% |
| ! | TTGAGAACAATCAGAATTTA+AGG | + | chr3.2:27610617-27610636 | None:intergenic | 25.0% |
| !! | TTCATTTTCAACAATTTGGA+TGG | + | chr3.2:27609866-27609885 | None:intergenic | 25.0% |
| !!! | GTTTGATTTTCAGCTAAAAT+AGG | + | chr3.2:27610394-27610413 | None:intergenic | 25.0% |
| AAATCTGGAAATAGAAGAGA+AGG | + | chr3.2:27609666-27609685 | None:intergenic | 30.0% | |
| AACTCTATTCCTCAAGTAAA+AGG | - | chr3.2:27610286-27610305 | MS.gene50973:intron | 30.0% | |
| AATTCTCCATAAACATGAGA+GGG | + | chr3.2:27610726-27610745 | None:intergenic | 30.0% | |
| ACAAGTACAACGTTTGTATA+TGG | + | chr3.2:27609385-27609404 | None:intergenic | 30.0% | |
| ACTATTGGAATACACATTTG+AGG | - | chr3.2:27610212-27610231 | MS.gene50973:intron | 30.0% | |
| ACTTCAAACAGATATCCATA+TGG | - | chr3.2:27610324-27610343 | MS.gene50973:intron | 30.0% | |
| AGAAAAAAGTTACTTGGCAA+CGG | - | chr3.2:27610651-27610670 | MS.gene50973:intron | 30.0% | |
| AGATCTAATGAACGTTTCAA+TGG | - | chr3.2:27610780-27610799 | MS.gene50973:CDS | 30.0% | |
| ATATACAAACGTTGTACTTG+TGG | - | chr3.2:27609384-27609403 | MS.gene50973:CDS | 30.0% | |
| CCTTACAAGATGATATTGTA+CGG | - | chr3.2:27610033-27610052 | MS.gene50973:intron | 30.0% | |
| CTTAAGAAGATCAATCAAAC+AGG | - | chr3.2:27610241-27610260 | MS.gene50973:intron | 30.0% | |
| CTTACAAGATGATATTGTAC+GGG | - | chr3.2:27610034-27610053 | MS.gene50973:intron | 30.0% | |
| GATGAAAAAACCCGAAAATT+CGG | - | chr3.2:27610465-27610484 | MS.gene50973:intron | 30.0% | |
| GGTTGAAGAAAAAAGTTACT+TGG | - | chr3.2:27610645-27610664 | MS.gene50973:intron | 30.0% | |
| GTATCATACATTCAAGAACA+TGG | - | chr3.2:27609074-27609093 | MS.gene50973:CDS | 30.0% | |
| TGTTTATAAGACAAATTGGC+TGG | + | chr3.2:27609770-27609789 | None:intergenic | 30.0% | |
| TTTCAGTGTTTGATGCATAT+GGG | + | chr3.2:27610420-27610439 | None:intergenic | 30.0% | |
| ! | AAATTACCTCTTTTGATACC+TGG | + | chr3.2:27609531-27609550 | None:intergenic | 30.0% |
| ! | TTAGCCTATATTTGAAACCA+CGG | + | chr3.2:27609344-27609363 | None:intergenic | 30.0% |
| ! | TTTTCAACAATTTGGATGGA+AGG | + | chr3.2:27609862-27609881 | None:intergenic | 30.0% |
| ! | TTTTCAGCTAAAATAGGTGT+TGG | + | chr3.2:27610388-27610407 | None:intergenic | 30.0% |
| ! | TTTTCAGTGTTTGATGCATA+TGG | + | chr3.2:27610421-27610440 | None:intergenic | 30.0% |
| !!! | TGAATTGTTTTAACCTGTAG+TGG | + | chr3.2:27609126-27609145 | None:intergenic | 30.0% |
| AACTCCTTTATGGTAGTTAC+AGG | - | chr3.2:27610508-27610527 | MS.gene50973:intron | 35.0% | |
| ACCAAAATTATATCCTCCTC+TGG | + | chr3.2:27609056-27609075 | None:intergenic | 35.0% | |
| AGATCCTGTAACTACCATAA+AGG | + | chr3.2:27610515-27610534 | None:intergenic | 35.0% | |
| ATATGAAAGTAAGCCAAACC+AGG | - | chr3.2:27610690-27610709 | MS.gene50973:intron | 35.0% | |
| ATGTCATTGTCTGTTCTTTC+TGG | + | chr3.2:27610187-27610206 | None:intergenic | 35.0% | |
| ATTACTAAGATCGTCCGATT+TGG | - | chr3.2:27609915-27609934 | MS.gene50973:intron | 35.0% | |
| CAATTCTCCATAAACATGAG+AGG | + | chr3.2:27610727-27610746 | None:intergenic | 35.0% | |
| CCGTACAATATCATCTTGTA+AGG | + | chr3.2:27610036-27610055 | None:intergenic | 35.0% | |
| CTCTCATGTTTATGGAGAAT+TGG | - | chr3.2:27610725-27610744 | MS.gene50973:CDS | 35.0% | |
| GAAAAAAGTTACTTGGCAAC+GGG | - | chr3.2:27610652-27610671 | MS.gene50973:intron | 35.0% | |
| GAGAAAATTGGGGTTAAGAA+AGG | - | chr3.2:27609020-27609039 | MS.gene50973:CDS | 35.0% | |
| GTAAAAGTTGCAGACTTAGA+TGG | - | chr3.2:27609490-27609509 | MS.gene50973:CDS | 35.0% | |
| TACCGCAACAATATCCAAAT+CGG | + | chr3.2:27609932-27609951 | None:intergenic | 35.0% | |
| TAGCTAGATTACTCGAAAAG+TGG | - | chr3.2:27610443-27610462 | MS.gene50973:intron | 35.0% | |
| TCCTCAAGTAAAAGGTCAAT+GGG | - | chr3.2:27610294-27610313 | MS.gene50973:intron | 35.0% | |
| TCTAATGAACGTTTCAATGG+AGG | - | chr3.2:27610783-27610802 | MS.gene50973:CDS | 35.0% | |
| TCTACCGTGGTTTCAAATAT+AGG | - | chr3.2:27609337-27609356 | MS.gene50973:CDS | 35.0% | |
| TGATTGTTCTCAATCTGTGT+CGG | - | chr3.2:27610624-27610643 | MS.gene50973:intron | 35.0% | |
| TGGACTAATTATCTTAGACC+AGG | - | chr3.2:27609510-27609529 | MS.gene50973:CDS | 35.0% | |
| TTCAGTGTTTGATGCATATG+GGG | + | chr3.2:27610419-27610438 | None:intergenic | 35.0% | |
| TTCCTCAAGTAAAAGGTCAA+TGG | - | chr3.2:27610293-27610312 | MS.gene50973:intron | 35.0% | |
| ! | TCCAGAGGAGGATATAATTT+TGG | - | chr3.2:27609052-27609071 | MS.gene50973:CDS | 35.0% |
| ! | TGTTGTGTCATCCGAATTTT+CGG | + | chr3.2:27610479-27610498 | None:intergenic | 35.0% |
| !! | ACAAAGCTGAAGCTATTTCT+TGG | + | chr3.2:27609225-27609244 | None:intergenic | 35.0% |
| !! | TATTTCCTAAAAGCGCTTGA+AGG | + | chr3.2:27609584-27609603 | None:intergenic | 35.0% |
| !! | TTCAAGCGCTTTTAGGAAAT+AGG | - | chr3.2:27609583-27609602 | MS.gene50973:CDS | 35.0% |
| !!! | ACAAAGCTTGTTTTGCCATA+TGG | + | chr3.2:27610342-27610361 | None:intergenic | 35.0% |
| !!! | ATTGTTTTAACCTGTAGTGG+TGG | + | chr3.2:27609123-27609142 | None:intergenic | 35.0% |
| !!! | TTGTTTTAACCTGTAGTGGT+GGG | + | chr3.2:27609122-27609141 | None:intergenic | 35.0% |
| AAGAACATGGACCTGGAAAT+TGG | - | chr3.2:27609087-27609106 | MS.gene50973:CDS | 40.0% | |
| ACAACGTTTGTATATGGCCA+GGG | + | chr3.2:27609379-27609398 | None:intergenic | 40.0% | |
| CAACAATTTGGATGGAAGGA+CGG | + | chr3.2:27609858-27609877 | None:intergenic | 40.0% | |
| CAATGGAGGAAAATACAGCA+GGG | - | chr3.2:27610797-27610816 | MS.gene50973:CDS | 40.0% | |
| CTTAGACCAGGTATCAAAAG+AGG | - | chr3.2:27609522-27609541 | MS.gene50973:CDS | 40.0% | |
| GCTAAATTGTTATCGATCCC+TGG | - | chr3.2:27609359-27609378 | MS.gene50973:CDS | 40.0% | |
| TACAACGTTTGTATATGGCC+AGG | + | chr3.2:27609380-27609399 | None:intergenic | 40.0% | |
| TACATTCAAGAACATGGACC+TGG | - | chr3.2:27609080-27609099 | MS.gene50973:CDS | 40.0% | |
| TCAAGTCCCTCTCATGTTTA+TGG | - | chr3.2:27610717-27610736 | MS.gene50973:CDS | 40.0% | |
| TCAATGGAGGAAAATACAGC+AGG | - | chr3.2:27610796-27610815 | MS.gene50973:CDS | 40.0% | |
| ! | ACCTTGCTGTGAGAAAATTG+GGG | - | chr3.2:27609010-27609029 | MS.gene50973:CDS | 40.0% |
| ! | ATTCACCTTCAAGCGCTTTT+AGG | - | chr3.2:27609576-27609595 | MS.gene50973:CDS | 40.0% |
| ! | CACCTTGCTGTGAGAAAATT+GGG | - | chr3.2:27609009-27609028 | MS.gene50973:CDS | 40.0% |
| ! | GTCTGTTCTTTCTGGAAGAT+AGG | + | chr3.2:27610179-27610198 | None:intergenic | 40.0% |
| ! | GTTGTGTCATCCGAATTTTC+GGG | + | chr3.2:27610478-27610497 | None:intergenic | 40.0% |
| ! | TCCCATTGACCTTTTACTTG+AGG | + | chr3.2:27610298-27610317 | None:intergenic | 40.0% |
| !! | ACAGGATCTAGTTCTAGTGA+AGG | - | chr3.2:27610526-27610545 | MS.gene50973:intron | 40.0% |
| !! | GTCCGATTTGGATATTGTTG+CGG | - | chr3.2:27609927-27609946 | MS.gene50973:intron | 40.0% |
| !!! | GAATTGGCCTTTTGATGAAG+AGG | - | chr3.2:27610741-27610760 | MS.gene50973:CDS | 40.0% |
| GGAACTGATCTCCAATTTCC+AGG | + | chr3.2:27609101-27609120 | None:intergenic | 45.0% | |
| TCATAGCTTCTACTCTACCG+TGG | - | chr3.2:27609324-27609343 | MS.gene50973:CDS | 45.0% | |
| TGAGCAGCCTCTTCATCAAA+AGG | + | chr3.2:27610751-27610770 | None:intergenic | 45.0% | |
| TTGGGGTTAAGAAAGGACCT+TGG | - | chr3.2:27609027-27609046 | MS.gene50973:CDS | 45.0% | |
| ! | ACCCCAATTTTCTCACAGCA+AGG | + | chr3.2:27609014-27609033 | None:intergenic | 45.0% |
| ! | CCAATTTTCTCACAGCAAGG+TGG | + | chr3.2:27609011-27609030 | None:intergenic | 45.0% |
| ! | CCACCTTGCTGTGAGAAAAT+TGG | - | chr3.2:27609008-27609027 | MS.gene50973:CDS | 45.0% |
| ! | GGCAAAACAAGCTTTGTGTG+AGG | - | chr3.2:27610345-27610364 | MS.gene50973:intron | 45.0% |
| !! | GGATCTAGTTCTAGTGAAGG+AGG | - | chr3.2:27610529-27610548 | MS.gene50973:intron | 45.0% |
| AGATCAGTTCCCACCACTAC+AGG | - | chr3.2:27609110-27609129 | MS.gene50973:CDS | 50.0% | |
| GGGACTTGAGTTTCCTGGTT+TGG | + | chr3.2:27610706-27610725 | None:intergenic | 50.0% | |
| TGAGAGGGACTTGAGTTTCC+TGG | + | chr3.2:27610711-27610730 | None:intergenic | 50.0% | |
| !! | ATATCCTCCTCTGGAGTCCA+AGG | + | chr3.2:27609047-27609066 | None:intergenic | 50.0% |
| GAAAGGACCTTGGACTCCAG+AGG | - | chr3.2:27609037-27609056 | MS.gene50973:CDS | 55.0% | |
| AGGACCTTGGACTCCAGAGG+AGG | - | chr3.2:27609040-27609059 | MS.gene50973:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 27608999 | 27610828 | 27608999 | ID=MS.gene50973 |
| chr3.2 | mRNA | 27608999 | 27610828 | 27608999 | ID=MS.gene50973.t1;Parent=MS.gene50973 |
| chr3.2 | exon | 27610696 | 27610828 | 27610696 | ID=MS.gene50973.t1.exon1;Parent=MS.gene50973.t1 |
| chr3.2 | CDS | 27610696 | 27610828 | 27610696 | ID=cds.MS.gene50973.t1;Parent=MS.gene50973.t1 |
| chr3.2 | exon | 27608999 | 27609639 | 27608999 | ID=MS.gene50973.t1.exon2;Parent=MS.gene50973.t1 |
| chr3.2 | CDS | 27608999 | 27609639 | 27608999 | ID=cds.MS.gene50973.t1;Parent=MS.gene50973.t1 |
| Gene Sequence |
| Protein sequence |
