Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51053.t1 | KEH31907.1 | 77.8 | 54 | 7 | 1 | 1 | 54 | 1 | 49 | 5.00E-11 | 76.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51053.t1 | G7JPX2 | 77.8 | 54 | 7 | 1 | 1 | 54 | 1 | 49 | 3.6e-11 | 76.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052307 | MS.gene51053 | 0.80201 | 6.87E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene51053 | 0.800742 | 1.25E-48 | -1.69E-46 |
| MS.gene059170 | MS.gene51053 | 0.802835 | 4.65E-49 | -1.69E-46 |
| MS.gene060373 | MS.gene51053 | 0.804378 | 2.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51053.t1 | MTR_4g107820 | 77.778 | 54 | 7 | 1 | 1 | 54 | 1 | 49 | 1.93e-21 | 84.7 |
| MS.gene51053.t1 | MTR_4g107780 | 77.778 | 54 | 7 | 1 | 1 | 54 | 1 | 49 | 1.93e-21 | 84.7 |
| MS.gene51053.t1 | MTR_4g107780 | 77.778 | 54 | 7 | 1 | 1 | 54 | 1 | 49 | 2.45e-21 | 84.7 |
| MS.gene51053.t1 | MTR_4g107820 | 77.778 | 54 | 7 | 1 | 1 | 54 | 1 | 49 | 2.76e-21 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCATTTCTTCCGGAAATAT+CGG | 0.302071 | 8.4:-19276617 | None:intergenic |
| GATGCCGTCGCCGATATTTC+CGG | 0.344496 | 8.4:+19276607 | MS.gene51053:CDS |
| TGAGATACTCCTTCGACTTC+CGG | 0.357501 | 8.4:+19276643 | MS.gene51053:CDS |
| ATCTCAACGATCATTTCTTC+CGG | 0.399062 | 8.4:-19276626 | None:intergenic |
| CGTCACCGTCTTTACTCAAT+CGG | 0.512976 | 8.4:+19276565 | MS.gene51053:CDS |
| CGGCGACGGCATCTCGGCGG+TGG | 0.532801 | 8.4:-19276597 | None:intergenic |
| GTGAGAATCGTTCAAGGACA+AGG | 0.541241 | 8.4:+19276516 | MS.gene51053:CDS |
| AAATATCGGCGACGGCATCT+CGG | 0.550932 | 8.4:-19276603 | None:intergenic |
| TCTTCCGGAAATATCGGCGA+CGG | 0.610122 | 8.4:-19276611 | None:intergenic |
| GGGATCTCACCGGAAGTCGA+AGG | 0.615230 | 8.4:-19276652 | None:intergenic |
| CGTCTTTACTCAATCGGTGT+CGG | 0.628117 | 8.4:+19276571 | MS.gene51053:CDS |
| CGACACCGATTGAGTAAAGA+CGG | 0.630780 | 8.4:-19276570 | None:intergenic |
| TATCGGCGACGGCATCTCGG+CGG | 0.706688 | 8.4:-19276600 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCTCACAAAATGTTATCAT+CGG | - | chr8.4:19276503-19276522 | None:intergenic | 25.0% |
| ATCATTTCTTCCGGAAATAT+CGG | - | chr8.4:19276620-19276639 | None:intergenic | 30.0% | |
| ATCTCAACGATCATTTCTTC+CGG | - | chr8.4:19276629-19276648 | None:intergenic | 35.0% | |
| ! | CATTTTGTGAGAATCGTTCA+AGG | + | chr8.4:19276510-19276529 | MS.gene51053:CDS | 35.0% |
| CGTCACCGTCTTTACTCAAT+CGG | + | chr8.4:19276565-19276584 | MS.gene51053:CDS | 45.0% | |
| CGTCTTTACTCAATCGGTGT+CGG | + | chr8.4:19276571-19276590 | MS.gene51053:CDS | 45.0% | |
| GTGAGAATCGTTCAAGGACA+AGG | + | chr8.4:19276516-19276535 | MS.gene51053:CDS | 45.0% | |
| ! | CGACACCGATTGAGTAAAGA+CGG | - | chr8.4:19276573-19276592 | None:intergenic | 45.0% |
| AAATATCGGCGACGGCATCT+CGG | - | chr8.4:19276606-19276625 | None:intergenic | 50.0% | |
| TCTTCCGGAAATATCGGCGA+CGG | - | chr8.4:19276614-19276633 | None:intergenic | 50.0% | |
| GATGCCGTCGCCGATATTTC+CGG | + | chr8.4:19276607-19276626 | MS.gene51053:CDS | 55.0% | |
| TATCGGCGACGGCATCTCGG+CGG | - | chr8.4:19276603-19276622 | None:intergenic | 65.0% | |
| ! | CGGCGACGGCATCTCGGCGG+TGG | - | chr8.4:19276600-19276619 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 19276503 | 19276664 | 19276503 | ID=MS.gene51053 |
| chr8.4 | mRNA | 19276503 | 19276664 | 19276503 | ID=MS.gene51053.t1;Parent=MS.gene51053 |
| chr8.4 | exon | 19276503 | 19276664 | 19276503 | ID=MS.gene51053.t1.exon1;Parent=MS.gene51053.t1 |
| chr8.4 | CDS | 19276503 | 19276664 | 19276503 | ID=cds.MS.gene51053.t1;Parent=MS.gene51053.t1 |
| Gene Sequence |
| Protein sequence |
