Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51080.t1 | XP_003616694.1 | 95.6 | 205 | 9 | 0 | 1 | 205 | 1 | 205 | 2.90E-107 | 397.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51080.t1 | O80920 | 72.7 | 165 | 44 | 1 | 39 | 203 | 32 | 195 | 1.6e-61 | 237.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51080.t1 | G7K772 | 95.6 | 205 | 9 | 0 | 1 | 205 | 1 | 205 | 2.1e-107 | 397.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052795 | MS.gene51080 | 0.850322 | 1.83E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51080.t1 | MTR_5g083270 | 95.610 | 205 | 9 | 0 | 1 | 205 | 1 | 205 | 2.50e-146 | 405 |
| MS.gene51080.t1 | MTR_3g071740 | 62.946 | 224 | 62 | 5 | 1 | 204 | 1 | 223 | 5.46e-84 | 248 |
| MS.gene51080.t1 | MTR_4g014460 | 64.327 | 171 | 54 | 4 | 39 | 205 | 42 | 209 | 1.17e-71 | 216 |
| MS.gene51080.t1 | MTR_7g070050 | 68.000 | 175 | 46 | 4 | 39 | 205 | 6 | 178 | 1.98e-70 | 213 |
| MS.gene51080.t1 | MTR_5g030500 | 55.488 | 164 | 66 | 3 | 46 | 203 | 44 | 206 | 2.16e-60 | 188 |
| MS.gene51080.t1 | MTR_8g027805 | 52.353 | 170 | 73 | 4 | 41 | 205 | 11 | 177 | 7.23e-58 | 181 |
| MS.gene51080.t1 | MTR_3g090980 | 51.534 | 163 | 73 | 3 | 44 | 203 | 19 | 178 | 2.94e-57 | 179 |
| MS.gene51080.t1 | MTR_1g028380 | 50.909 | 165 | 75 | 2 | 44 | 205 | 17 | 178 | 8.67e-57 | 178 |
| MS.gene51080.t1 | MTR_1g016480 | 52.795 | 161 | 69 | 3 | 46 | 202 | 39 | 196 | 1.86e-54 | 172 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51080.t1 | AT2G40330 | 58.571 | 210 | 79 | 3 | 1 | 203 | 1 | 209 | 1.57e-81 | 242 |
| MS.gene51080.t1 | AT5G05440 | 60.396 | 202 | 74 | 3 | 1 | 200 | 1 | 198 | 7.60e-77 | 229 |
| MS.gene51080.t1 | AT2G38310 | 65.285 | 193 | 60 | 3 | 13 | 203 | 8 | 195 | 2.19e-73 | 221 |
| MS.gene51080.t1 | AT1G01360 | 52.147 | 163 | 72 | 2 | 46 | 205 | 21 | 180 | 1.79e-60 | 187 |
| MS.gene51080.t1 | AT4G27920 | 50.909 | 165 | 76 | 2 | 44 | 205 | 12 | 174 | 1.12e-57 | 180 |
| MS.gene51080.t1 | AT4G01026 | 52.201 | 159 | 70 | 3 | 50 | 205 | 27 | 182 | 3.45e-56 | 177 |
| MS.gene51080.t1 | AT5G53160 | 50.909 | 165 | 75 | 3 | 44 | 205 | 17 | 178 | 3.84e-56 | 176 |
| MS.gene51080.t1 | AT4G17870 | 50.610 | 164 | 77 | 2 | 45 | 205 | 16 | 178 | 3.80e-53 | 169 |
| MS.gene51080.t1 | AT2G26040 | 49.383 | 162 | 75 | 3 | 46 | 202 | 22 | 181 | 1.34e-50 | 162 |
| MS.gene51080.t1 | AT1G73000 | 47.619 | 168 | 77 | 3 | 46 | 202 | 37 | 204 | 9.96e-50 | 160 |
| MS.gene51080.t1 | AT5G46790 | 51.765 | 170 | 75 | 2 | 42 | 205 | 40 | 208 | 8.96e-49 | 159 |
| MS.gene51080.t1 | AT5G46790 | 51.765 | 170 | 75 | 2 | 42 | 205 | 40 | 208 | 8.96e-49 | 159 |
| MS.gene51080.t1 | AT5G45860 | 58.389 | 149 | 58 | 3 | 59 | 205 | 10 | 156 | 1.16e-46 | 151 |
| MS.gene51080.t1 | AT5G45870 | 56.954 | 151 | 62 | 3 | 56 | 205 | 7 | 155 | 2.10e-45 | 148 |
| MS.gene51080.t1 | AT4G18620 | 46.914 | 162 | 79 | 2 | 51 | 205 | 1 | 162 | 1.19e-35 | 123 |
| MS.gene51080.t1 | AT5G53160 | 46.667 | 105 | 53 | 2 | 44 | 148 | 17 | 118 | 5.38e-28 | 103 |
Find 59 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTGGTTTGCAACCGTTTC+TGG | 0.080678 | 5.1:+65604556 | None:intergenic |
| CGTGAAGTTCGACTTGTTTC+CGG | 0.131980 | 5.1:-65604358 | MS.gene51080:CDS |
| AAACGGAGGCCTTGATGGTT+TGG | 0.349101 | 5.1:+65604491 | None:intergenic |
| GTGTTGTTGGTGGTGTTCAT+AGG | 0.363891 | 5.1:-65604267 | MS.gene51080:CDS |
| TCTAACACCGCCATGACTAA+TGG | 0.405607 | 5.1:-65604649 | MS.gene51080:CDS |
| GTTATTAGTTTCAGTGTTGT+TGG | 0.408321 | 5.1:-65604280 | MS.gene51080:CDS |
| TTCATAGGTGTAGGAATTAC+CGG | 0.411086 | 5.1:-65604252 | MS.gene51080:CDS |
| AGGCCTCCGTTTCAACTGTC+TGG | 0.430513 | 5.1:-65604480 | MS.gene51080:CDS |
| CGACACGCTTGAATTTGTTT+CGG | 0.430906 | 5.1:+65604616 | None:intergenic |
| AGTTGAAACGGAGGCCTTGA+TGG | 0.442287 | 5.1:+65604486 | None:intergenic |
| TCGGTGTAATTTGCAGTCAC+TGG | 0.459352 | 5.1:-65604110 | MS.gene51080:CDS |
| GGTGGTTTGCAACCGTTTCT+GGG | 0.462922 | 5.1:+65604557 | None:intergenic |
| AACGTAGTTGCCTCCGGTGA+TGG | 0.485651 | 5.1:-65604400 | MS.gene51080:CDS |
| AACCGTTTCTGTCCCAGAAA+CGG | 0.488981 | 5.1:-65604569 | MS.gene51080:CDS |
| AGCTGCAACGTAGTTGCCTC+CGG | 0.500297 | 5.1:-65604406 | MS.gene51080:CDS |
| GCTTGAAACGGCAGGTAGAC+CGG | 0.502003 | 5.1:+65604339 | None:intergenic |
| CAACCTCTCCATGCTTGAAA+CGG | 0.516613 | 5.1:+65604327 | None:intergenic |
| GGATCACGACGGAGTAGCAC+TGG | 0.520709 | 5.1:+65604512 | None:intergenic |
| AACCGTTTCTGGGACAGAAA+CGG | 0.521454 | 5.1:+65604567 | None:intergenic |
| CGTTTCAAGCATGGAGAGGT+TGG | 0.529991 | 5.1:-65604326 | MS.gene51080:CDS |
| TCGAACCGTTGAAATTGAAC+CGG | 0.530260 | 5.1:+65604673 | None:intergenic |
| CGTTGAAATTGAACCGGTGA+AGG | 0.532412 | 5.1:+65604679 | None:intergenic |
| ACCGACACGAATGCCATCAC+CGG | 0.534492 | 5.1:+65604387 | None:intergenic |
| AACGGACCAGACAGTTGAAA+CGG | 0.535986 | 5.1:+65604474 | None:intergenic |
| CAAAATAGAAATGCCTTCAC+CGG | 0.537348 | 5.1:-65604692 | None:intergenic |
| CTTCACCGGTTCAATTTCAA+CGG | 0.537786 | 5.1:-65604678 | MS.gene51080:CDS |
| CTCTCCATGCTTGAAACGGC+AGG | 0.544665 | 5.1:+65604331 | None:intergenic |
| ACAAAGTGTTTGTAGCCTTG+TGG | 0.546590 | 5.1:+65604433 | None:intergenic |
| TCTACCTGCCGTTTCAAGCA+TGG | 0.550915 | 5.1:-65604335 | MS.gene51080:CDS |
| CGGTCAGTGACGACGTTGCA+CGG | 0.551799 | 5.1:-65604232 | MS.gene51080:CDS |
| TTGGTGGTGTTCATAGGTGT+AGG | 0.559793 | 5.1:-65604261 | MS.gene51080:CDS |
| ATTAGTTTCAGTGTTGTTGG+TGG | 0.561597 | 5.1:-65604277 | MS.gene51080:CDS |
| CGTCGTGATCCAAACCATCA+AGG | 0.571418 | 5.1:-65604500 | MS.gene51080:CDS |
| ATACGTCGTTGATGTACCAC+AGG | 0.575028 | 5.1:-65604176 | MS.gene51080:CDS |
| CGTGCAACGTCGTCACTGAC+CGG | 0.576112 | 5.1:+65604233 | None:intergenic |
| CAACACTGAAACTAATAACA+TGG | 0.585088 | 5.1:+65604284 | None:intergenic |
| ACAGTTAATGCCATTAGTCA+TGG | 0.586782 | 5.1:+65604639 | None:intergenic |
| ACGTTGCACGGTGACGGTAA+CGG | 0.591404 | 5.1:-65604220 | MS.gene51080:CDS |
| GTGACGACGTTGCACGGTGA+CGG | 0.594931 | 5.1:-65604226 | MS.gene51080:CDS |
| AGCCTTGTGGATTGTCGAAG+CGG | 0.601019 | 5.1:+65604446 | None:intergenic |
| AACCACCACATGCACGTGGT+GGG | 0.602296 | 5.1:-65604541 | MS.gene51080:CDS |
| TCCGGTGATGGCATTCGTGT+CGG | 0.606951 | 5.1:-65604388 | MS.gene51080:CDS |
| TGTACCACAGGGTAACACTA+AGG | 0.609377 | 5.1:-65604164 | MS.gene51080:CDS |
| CGCCGCTTCGACAATCCACA+AGG | 0.611657 | 5.1:-65604448 | MS.gene51080:CDS |
| AAACCACCACATGCACGTGG+TGG | 0.622421 | 5.1:-65604542 | MS.gene51080:CDS |
| GTCCCACCACGTGCATGTGG+TGG | 0.623392 | 5.1:+65604539 | None:intergenic |
| CTGCCGTTTCAAGCATGGAG+AGG | 0.630256 | 5.1:-65604330 | MS.gene51080:CDS |
| ATTGTCGAAGCGGCGGACAA+CGG | 0.630831 | 5.1:+65604456 | None:intergenic |
| CTGCAAATTACACCGAACAA+TGG | 0.632705 | 5.1:+65604117 | None:intergenic |
| TGCAAACCACCACATGCACG+TGG | 0.642151 | 5.1:-65604545 | MS.gene51080:CDS |
| TTTGTCCCACCACGTGCATG+TGG | 0.645948 | 5.1:+65604536 | None:intergenic |
| TCTTCCTTAGTGTTACCCTG+TGG | 0.656284 | 5.1:+65604160 | None:intergenic |
| GACACGAATGCCATCACCGG+AGG | 0.657865 | 5.1:+65604390 | None:intergenic |
| CTTGTGGATTGTCGAAGCGG+CGG | 0.689521 | 5.1:+65604449 | None:intergenic |
| CTTGATGGTTTGGATCACGA+CGG | 0.697791 | 5.1:+65604501 | None:intergenic |
| GGACCAGACAGTTGAAACGG+AGG | 0.712986 | 5.1:+65604477 | None:intergenic |
| GTTAATGCCATTAGTCATGG+CGG | 0.726535 | 5.1:+65604642 | None:intergenic |
| TTGCACGGTGACGGTAACGG+AGG | 0.729282 | 5.1:-65604217 | MS.gene51080:CDS |
| TACGTCGTTGATGTACCACA+GGG | 0.754269 | 5.1:-65604175 | MS.gene51080:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAACACTGAAACTAATAACA+TGG | + | chr5.1:65604488-65604507 | None:intergenic | 30.0% | |
| ! | GTTATTAGTTTCAGTGTTGT+TGG | - | chr5.1:65604489-65604508 | MS.gene51080:CDS | 30.0% |
| !! | GTTTTGTTGATACCATTGTT+CGG | - | chr5.1:65604640-65604659 | MS.gene51080:CDS | 30.0% |
| ACAGTTAATGCCATTAGTCA+TGG | + | chr5.1:65604133-65604152 | None:intergenic | 35.0% | |
| TTCATAGGTGTAGGAATTAC+CGG | - | chr5.1:65604517-65604536 | MS.gene51080:CDS | 35.0% | |
| ! | ATTAGTTTCAGTGTTGTTGG+TGG | - | chr5.1:65604492-65604511 | MS.gene51080:CDS | 35.0% |
| CTGCAAATTACACCGAACAA+TGG | + | chr5.1:65604655-65604674 | None:intergenic | 40.0% | |
| GTTAATGCCATTAGTCATGG+CGG | + | chr5.1:65604130-65604149 | None:intergenic | 40.0% | |
| TCGAACCGTTGAAATTGAAC+CGG | + | chr5.1:65604099-65604118 | None:intergenic | 40.0% | |
| ! | ACAAAGTGTTTGTAGCCTTG+TGG | + | chr5.1:65604339-65604358 | None:intergenic | 40.0% |
| ! | CGACACGCTTGAATTTGTTT+CGG | + | chr5.1:65604156-65604175 | None:intergenic | 40.0% |
| !! | CTTCACCGGTTCAATTTCAA+CGG | - | chr5.1:65604091-65604110 | MS.gene51080:CDS | 40.0% |
| AACCGTTTCTGTCCCAGAAA+CGG | - | chr5.1:65604200-65604219 | MS.gene51080:CDS | 45.0% | |
| AACGGACCAGACAGTTGAAA+CGG | + | chr5.1:65604298-65604317 | None:intergenic | 45.0% | |
| ATACGTCGTTGATGTACCAC+AGG | - | chr5.1:65604593-65604612 | MS.gene51080:CDS | 45.0% | |
| CAACCTCTCCATGCTTGAAA+CGG | + | chr5.1:65604445-65604464 | None:intergenic | 45.0% | |
| CGTTGAAATTGAACCGGTGA+AGG | + | chr5.1:65604093-65604112 | None:intergenic | 45.0% | |
| TACGTCGTTGATGTACCACA+GGG | - | chr5.1:65604594-65604613 | MS.gene51080:CDS | 45.0% | |
| TCTAACACCGCCATGACTAA+TGG | - | chr5.1:65604120-65604139 | MS.gene51080:CDS | 45.0% | |
| TCTTCCTTAGTGTTACCCTG+TGG | + | chr5.1:65604612-65604631 | None:intergenic | 45.0% | |
| TGTACCACAGGGTAACACTA+AGG | - | chr5.1:65604605-65604624 | MS.gene51080:CDS | 45.0% | |
| ! | CGTGAAGTTCGACTTGTTTC+CGG | - | chr5.1:65604411-65604430 | MS.gene51080:CDS | 45.0% |
| ! | CTTGATGGTTTGGATCACGA+CGG | + | chr5.1:65604271-65604290 | None:intergenic | 45.0% |
| ! | TCGGTGTAATTTGCAGTCAC+TGG | - | chr5.1:65604659-65604678 | MS.gene51080:CDS | 45.0% |
| !! | AACCGTTTCTGGGACAGAAA+CGG | + | chr5.1:65604205-65604224 | None:intergenic | 45.0% |
| !! | GTGTTGTTGGTGGTGTTCAT+AGG | - | chr5.1:65604502-65604521 | MS.gene51080:CDS | 45.0% |
| !! | TTGGTGGTGTTCATAGGTGT+AGG | - | chr5.1:65604508-65604527 | MS.gene51080:CDS | 45.0% |
| AGTTGAAACGGAGGCCTTGA+TGG | + | chr5.1:65604286-65604305 | None:intergenic | 50.0% | |
| CGTCGTGATCCAAACCATCA+AGG | - | chr5.1:65604269-65604288 | MS.gene51080:CDS | 50.0% | |
| CGTTTCAAGCATGGAGAGGT+TGG | - | chr5.1:65604443-65604462 | MS.gene51080:CDS | 50.0% | |
| ! | AAACGGAGGCCTTGATGGTT+TGG | + | chr5.1:65604281-65604300 | None:intergenic | 50.0% |
| ! | AGCCTTGTGGATTGTCGAAG+CGG | + | chr5.1:65604326-65604345 | None:intergenic | 50.0% |
| ! | GGTGGTTTGCAACCGTTTCT+GGG | + | chr5.1:65604215-65604234 | None:intergenic | 50.0% |
| ! | TCTACCTGCCGTTTCAAGCA+TGG | - | chr5.1:65604434-65604453 | MS.gene51080:CDS | 50.0% |
| ! | TGGTGGTTTGCAACCGTTTC+TGG | + | chr5.1:65604216-65604235 | None:intergenic | 50.0% |
| AAACCACCACATGCACGTGG+TGG | - | chr5.1:65604227-65604246 | MS.gene51080:CDS | 55.0% | |
| AACCACCACATGCACGTGGT+GGG | - | chr5.1:65604228-65604247 | MS.gene51080:CDS | 55.0% | |
| AACGTAGTTGCCTCCGGTGA+TGG | - | chr5.1:65604369-65604388 | MS.gene51080:CDS | 55.0% | |
| ACCGACACGAATGCCATCAC+CGG | + | chr5.1:65604385-65604404 | None:intergenic | 55.0% | |
| AGCTGCAACGTAGTTGCCTC+CGG | - | chr5.1:65604363-65604382 | MS.gene51080:CDS | 55.0% | |
| AGGCCTCCGTTTCAACTGTC+TGG | - | chr5.1:65604289-65604308 | MS.gene51080:CDS | 55.0% | |
| ATTGTCGAAGCGGCGGACAA+CGG | + | chr5.1:65604316-65604335 | None:intergenic | 55.0% | |
| CTCTCCATGCTTGAAACGGC+AGG | + | chr5.1:65604441-65604460 | None:intergenic | 55.0% | |
| GCTTGAAACGGCAGGTAGAC+CGG | + | chr5.1:65604433-65604452 | None:intergenic | 55.0% | |
| GGACCAGACAGTTGAAACGG+AGG | + | chr5.1:65604295-65604314 | None:intergenic | 55.0% | |
| TGCAAACCACCACATGCACG+TGG | - | chr5.1:65604224-65604243 | MS.gene51080:CDS | 55.0% | |
| TTTGTCCCACCACGTGCATG+TGG | + | chr5.1:65604236-65604255 | None:intergenic | 55.0% | |
| ! | ACGTTGCACGGTGACGGTAA+CGG | - | chr5.1:65604549-65604568 | MS.gene51080:CDS | 55.0% |
| ! | CTGCCGTTTCAAGCATGGAG+AGG | - | chr5.1:65604439-65604458 | MS.gene51080:CDS | 55.0% |
| ! | CTTGTGGATTGTCGAAGCGG+CGG | + | chr5.1:65604323-65604342 | None:intergenic | 55.0% |
| ! | TCCGGTGATGGCATTCGTGT+CGG | - | chr5.1:65604381-65604400 | MS.gene51080:CDS | 55.0% |
| CGCCGCTTCGACAATCCACA+AGG | - | chr5.1:65604321-65604340 | MS.gene51080:CDS | 60.0% | |
| CGGTCAGTGACGACGTTGCA+CGG | - | chr5.1:65604537-65604556 | MS.gene51080:CDS | 60.0% | |
| CGTGCAACGTCGTCACTGAC+CGG | + | chr5.1:65604539-65604558 | None:intergenic | 60.0% | |
| GACACGAATGCCATCACCGG+AGG | + | chr5.1:65604382-65604401 | None:intergenic | 60.0% | |
| GGATCACGACGGAGTAGCAC+TGG | + | chr5.1:65604260-65604279 | None:intergenic | 60.0% | |
| GTGACGACGTTGCACGGTGA+CGG | - | chr5.1:65604543-65604562 | MS.gene51080:CDS | 60.0% | |
| ! | TTGCACGGTGACGGTAACGG+AGG | - | chr5.1:65604552-65604571 | MS.gene51080:CDS | 60.0% |
| GTCCCACCACGTGCATGTGG+TGG | + | chr5.1:65604233-65604252 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 65604087 | 65604704 | 65604087 | ID=MS.gene51080 |
| chr5.1 | mRNA | 65604087 | 65604704 | 65604087 | ID=MS.gene51080.t1;Parent=MS.gene51080 |
| chr5.1 | exon | 65604087 | 65604704 | 65604087 | ID=MS.gene51080.t1.exon1;Parent=MS.gene51080.t1 |
| chr5.1 | CDS | 65604087 | 65604704 | 65604087 | ID=cds.MS.gene51080.t1;Parent=MS.gene51080.t1 |
| Gene Sequence |
| Protein sequence |
