AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052795


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052795.t1 MTR_4g014460 94.954 218 8 1 1 218 1 215 2.59e-150 416
MS.gene052795.t1 MTR_7g070050 65.775 187 56 3 36 215 1 186 8.20e-77 229
MS.gene052795.t1 MTR_5g083270 62.941 170 58 3 42 209 39 205 2.34e-70 213
MS.gene052795.t1 MTR_3g071740 61.988 171 60 4 47 216 64 230 4.13e-69 211
MS.gene052795.t1 MTR_1g028380 45.902 183 94 2 31 213 5 182 6.47e-55 174
MS.gene052795.t1 MTR_5g030500 53.659 164 72 2 49 208 44 207 6.48e-55 174
MS.gene052795.t1 MTR_8g027805 47.879 165 85 1 49 213 18 181 3.89e-54 172
MS.gene052795.t1 MTR_3g090980 44.086 186 98 3 29 213 4 184 5.55e-53 169
MS.gene052795.t1 MTR_1g016480 52.500 160 72 3 49 206 39 196 5.65e-53 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052795.t1 AT2G38310 67.052 173 53 2 47 218 37 206 3.87e-73 221
MS.gene052795.t1 AT5G05440 64.815 162 53 2 45 205 41 199 2.02e-70 214
MS.gene052795.t1 AT2G40330 59.064 171 65 3 45 211 44 213 2.73e-66 204
MS.gene052795.t1 AT5G53160 48.485 165 84 1 49 213 19 182 2.89e-56 177
MS.gene052795.t1 AT1G01360 47.531 162 84 1 52 213 24 184 1.12e-55 176
MS.gene052795.t1 AT4G01026 49.068 161 81 1 53 213 27 186 8.46e-54 172
MS.gene052795.t1 AT2G26040 49.398 166 82 1 43 206 16 181 1.49e-53 170
MS.gene052795.t1 AT4G27920 46.667 165 88 0 49 213 14 178 5.11e-52 166
MS.gene052795.t1 AT4G17870 48.810 168 85 1 45 211 13 180 6.68e-52 166
MS.gene052795.t1 AT1G73000 46.199 171 82 4 43 203 31 201 3.48e-44 147
MS.gene052795.t1 AT5G46790 49.412 170 80 3 45 209 40 208 9.56e-44 146
MS.gene052795.t1 AT5G46790 49.412 170 80 3 45 209 40 208 9.56e-44 146
MS.gene052795.t1 AT5G45860 52.055 146 69 1 64 209 12 156 4.35e-42 140
MS.gene052795.t1 AT5G45870 50.676 148 71 1 64 211 12 157 9.15e-42 139
MS.gene052795.t1 AT4G18620 45.963 161 80 2 58 211 4 164 2.07e-34 120
MS.gene052795.t1 AT5G53160 50.495 101 49 1 49 149 19 118 4.71e-32 114

Find 72 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGAAGACTTTGGTGGATT+TGG 0.227133 4.4:-86285083 None:intergenic
CGTTCGGTGCTGCGTGCAGC+AGG 0.323719 4.4:-86285431 None:intergenic
GGACGACCGCCGAGCATAAT+TGG 0.334740 4.4:-86285256 None:intergenic
TGATAGTGGGCTATAGTATT+AGG 0.336898 4.4:-86285212 None:intergenic
CAGCAGGGAGGCCAGATATA+AGG 0.373259 4.4:-86285415 None:intergenic
TTAGGAATGGTGTTGGTGAC+AGG 0.377606 4.4:-86285194 None:intergenic
TGATGGAGGCGGTTGTCTCT+TGG 0.388404 4.4:-86285277 None:intergenic
TCCACAGAGGGTGACTGTTC+CGG 0.392902 4.4:+86285554 MS.gene052795:CDS
AGGGAGGTCAACCTTATATC+TGG 0.399072 4.4:+86285404 MS.gene052795:CDS
CACTGACCAAACGGCGGTGA+TGG 0.428126 4.4:-86285294 None:intergenic
ATATTGAGTGTGTGTGATAG+TGG 0.451535 4.4:-86285226 None:intergenic
GAAGACTTTGGTGGATTTGG+AGG 0.457214 4.4:-86285080 None:intergenic
CCGCCTCCATCACCGCCGTT+TGG 0.479222 4.4:+86285288 MS.gene052795:CDS
ATCGAGGATCTCAAGGCGTT+CGG 0.483659 4.4:-86285447 None:intergenic
CTATGTAGTGGATATTCCTT+CGG 0.488058 4.4:+86285595 MS.gene052795:CDS
CGTTGGTGGCGACGGTGATG+TGG 0.489155 4.4:+86285373 MS.gene052795:CDS
GCGGCGAACCACTGACCAAA+CGG 0.490188 4.4:-86285303 None:intergenic
GCCGTTGAGGTCGTGGTGGT+TGG 0.491967 4.4:-86285147 None:intergenic
AGTCTTCACTTCTCCTCCAC+AGG 0.523366 4.4:+86285096 MS.gene052795:CDS
TGTTTGTTGATACAATTGTT+CGG 0.525160 4.4:+86285642 MS.gene052795:CDS
CATCACCGCCGTTTGGTCAG+TGG 0.527329 4.4:+86285295 MS.gene052795:CDS
TATAGTATTAGGAATGGTGT+TGG 0.528924 4.4:-86285201 None:intergenic
AGGAGAAGTGAAGACTTTGG+TGG 0.533916 4.4:-86285089 None:intergenic
ACGGTGATGTGGGCACACTC+AGG 0.539446 4.4:+86285384 MS.gene052795:CDS
CCAAACGGCGGTGATGGAGG+CGG 0.539681 4.4:-86285288 None:intergenic
TGTAACCTCGTTGGTGGCGA+CGG 0.546094 4.4:+86285365 MS.gene052795:CDS
ATCAAGAGTTGTAACCTCGT+TGG 0.547255 4.4:+86285356 MS.gene052795:CDS
GTTGGTGATGGTCGTAGTGG+TGG 0.548645 4.4:-86285129 None:intergenic
CGAAGGAATATCCACTACAT+AGG 0.552473 4.4:-86285594 None:intergenic
CGGTGATGTGGGCACACTCA+GGG 0.556265 4.4:+86285385 MS.gene052795:CDS
GTTCGGTGCTGCGTGCAGCA+GGG 0.556567 4.4:-86285430 None:intergenic
CAACACTAAAACTGATGACA+TGG 0.557856 4.4:-86285478 None:intergenic
GAGGTCGTGGTGGTTGGTGA+TGG 0.563623 4.4:-86285141 None:intergenic
GATCCTCATCGAGGATCTCA+AGG 0.566346 4.4:-86285454 None:intergenic
TAGGAATGGTGTTGGTGACA+GGG 0.566710 4.4:-86285193 None:intergenic
AAGAGTTGTAACCTCGTTGG+TGG 0.571438 4.4:+86285359 MS.gene052795:CDS
AGGCTTGTGGTTTGTCGAAG+CGG 0.573243 4.4:-86285322 None:intergenic
GGAACAGTCACCCTCTGTGG+AGG 0.574183 4.4:-86285552 None:intergenic
TGGTGACAGGGTTGATGAAG+AGG 0.574991 4.4:-86285181 None:intergenic
TGGTGGTGTGGTTGATCCTG+TGG 0.579437 4.4:-86285112 None:intergenic
TCCTTCGGAAAATACTAAAG+AGG 0.584494 4.4:+86285610 MS.gene052795:CDS
ATCATCCGACCAATTATGCT+CGG 0.584649 4.4:+86285247 MS.gene052795:CDS
TGACCAAACGGCGGTGATGG+AGG 0.585643 4.4:-86285291 None:intergenic
ACACTTCATCCCTCCACAGA+GGG 0.594198 4.4:+86285542 MS.gene052795:CDS
CGGTTTGTGCAAGTGACTGA+AGG 0.594264 4.4:-86285670 None:intergenic
GATAGTTGAGTCCTATGTAG+TGG 0.595701 4.4:+86285583 MS.gene052795:CDS
GGTGACAGGGTTGATGAAGA+GGG 0.596139 4.4:-86285180 None:intergenic
GCCGGAACAGTCACCCTCTG+TGG 0.599614 4.4:-86285555 None:intergenic
GGACTCAACTATCACCGTGC+CGG 0.603129 4.4:-86285573 None:intergenic
GAACAGTCACCCTCTGTGGA+GGG 0.606405 4.4:-86285551 None:intergenic
GACCGCCGAGCATAATTGGT+CGG 0.608055 4.4:-86285252 None:intergenic
GTGGGCTATAGTATTAGGAA+TGG 0.608376 4.4:-86285207 None:intergenic
GTTGGTGGCGACGGTGATGT+GGG 0.610157 4.4:+86285374 MS.gene052795:CDS
TGGAGGAGAAGTGAAGACTT+TGG 0.627794 4.4:-86285092 None:intergenic
ACCAACCACCACGACCTCAA+CGG 0.633346 4.4:+86285146 MS.gene052795:CDS
AGAGGGTGACTGTTCCGGCA+CGG 0.639448 4.4:+86285559 MS.gene052795:CDS
GGAGGGATGAAGTGTAGTAA+CGG 0.646842 4.4:-86285534 None:intergenic
TGATGGTCGTAGTGGTGGTG+TGG 0.646910 4.4:-86285124 None:intergenic
GTGGTTGGTGATGGTCGTAG+TGG 0.657655 4.4:-86285132 None:intergenic
CGGTGCTGCGTGCAGCAGGG+AGG 0.659975 4.4:-86285427 None:intergenic
TATTGAGTGTGTGTGATAGT+GGG 0.661022 4.4:-86285225 None:intergenic
TGGTGTGGTTGATCCTGTGG+AGG 0.662950 4.4:-86285109 None:intergenic
GATGAAGTGTAGTAACGGAA+CGG 0.672049 4.4:-86285529 None:intergenic
ACGCCTTGAGATCCTCGATG+AGG 0.696994 4.4:+86285451 MS.gene052795:CDS
ATCCGACCAATTATGCTCGG+CGG 0.704061 4.4:+86285250 MS.gene052795:CDS
TTGCTGTGTAATGTTCTCTG+CGG 0.706407 4.4:-86285690 None:intergenic
TTTCGCCGTTGAGGTCGTGG+TGG 0.717570 4.4:-86285151 None:intergenic
TGATGTGGGCACACTCAGGG+AGG 0.725955 4.4:+86285388 MS.gene052795:CDS
CATCACCGTCGCCACCAACG+AGG 0.726271 4.4:-86285370 None:intergenic
TACACTTCATCCCTCCACAG+AGG 0.732326 4.4:+86285541 MS.gene052795:CDS
TGACATGGCGATCCTCATCG+AGG 0.742190 4.4:-86285463 None:intergenic
GCGAACCACTGACCAAACGG+CGG 0.744614 4.4:-86285300 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTCTTGATGAAATTTTTGT+AGG - chr4.4:86285345-86285364 None:intergenic 25.0%
!! TGTTTGTTGATACAATTGTT+CGG + chr4.4:86285642-86285661 MS.gene052795:CDS 25.0%
TATAGTATTAGGAATGGTGT+TGG - chr4.4:86285204-86285223 None:intergenic 30.0%
! ATGAAATTTTTGTAGGCTTG+TGG - chr4.4:86285338-86285357 None:intergenic 30.0%
ATATTGAGTGTGTGTGATAG+TGG - chr4.4:86285229-86285248 None:intergenic 35.0%
CAACACTAAAACTGATGACA+TGG - chr4.4:86285481-86285500 None:intergenic 35.0%
TATTGAGTGTGTGTGATAGT+GGG - chr4.4:86285228-86285247 None:intergenic 35.0%
TCCTTCGGAAAATACTAAAG+AGG + chr4.4:86285610-86285629 MS.gene052795:CDS 35.0%
! TCCTCTTTAGTATTTTCCGA+AGG - chr4.4:86285614-86285633 None:intergenic 35.0%
!! ATCAGTTTTAGTGTTGTTGG+TGG + chr4.4:86285485-86285504 MS.gene052795:CDS 35.0%
!! CTATGTAGTGGATATTCCTT+CGG + chr4.4:86285595-86285614 MS.gene052795:CDS 35.0%
!! GTCATCAGTTTTAGTGTTGT+TGG + chr4.4:86285482-86285501 MS.gene052795:CDS 35.0%
!! TGATAGTGGGCTATAGTATT+AGG - chr4.4:86285215-86285234 None:intergenic 35.0%
ATCAAGAGTTGTAACCTCGT+TGG + chr4.4:86285356-86285375 MS.gene052795:CDS 40.0%
ATCATCCGACCAATTATGCT+CGG + chr4.4:86285247-86285266 MS.gene052795:CDS 40.0%
CGAAGGAATATCCACTACAT+AGG - chr4.4:86285597-86285616 None:intergenic 40.0%
GATAGTTGAGTCCTATGTAG+TGG + chr4.4:86285583-86285602 MS.gene052795:CDS 40.0%
TTGCTGTGTAATGTTCTCTG+CGG - chr4.4:86285693-86285712 None:intergenic 40.0%
! GATGAAGTGTAGTAACGGAA+CGG - chr4.4:86285532-86285551 None:intergenic 40.0%
! GTGGGCTATAGTATTAGGAA+TGG - chr4.4:86285210-86285229 None:intergenic 40.0%
!! AGTGAAGACTTTGGTGGATT+TGG - chr4.4:86285086-86285105 None:intergenic 40.0%
AAGAGTTGTAACCTCGTTGG+TGG + chr4.4:86285359-86285378 MS.gene052795:CDS 45.0%
AGGAGAAGTGAAGACTTTGG+TGG - chr4.4:86285092-86285111 None:intergenic 45.0%
AGGGAGGTCAACCTTATATC+TGG + chr4.4:86285404-86285423 MS.gene052795:CDS 45.0%
GGAGGGATGAAGTGTAGTAA+CGG - chr4.4:86285537-86285556 None:intergenic 45.0%
TGGAGGAGAAGTGAAGACTT+TGG - chr4.4:86285095-86285114 None:intergenic 45.0%
!! GAAGACTTTGGTGGATTTGG+AGG - chr4.4:86285083-86285102 None:intergenic 45.0%
!! TAGGAATGGTGTTGGTGACA+GGG - chr4.4:86285196-86285215 None:intergenic 45.0%
!! TTAGGAATGGTGTTGGTGAC+AGG - chr4.4:86285197-86285216 None:intergenic 45.0%
ACACTTCATCCCTCCACAGA+GGG + chr4.4:86285542-86285561 MS.gene052795:CDS 50.0%
AGTCTTCACTTCTCCTCCAC+AGG + chr4.4:86285096-86285115 MS.gene052795:CDS 50.0%
ATCCGACCAATTATGCTCGG+CGG + chr4.4:86285250-86285269 MS.gene052795:CDS 50.0%
CGGTTTGTGCAAGTGACTGA+AGG - chr4.4:86285673-86285692 None:intergenic 50.0%
GATCCTCATCGAGGATCTCA+AGG - chr4.4:86285457-86285476 None:intergenic 50.0%
TACACTTCATCCCTCCACAG+AGG + chr4.4:86285541-86285560 MS.gene052795:CDS 50.0%
! AGGCTTGTGGTTTGTCGAAG+CGG - chr4.4:86285325-86285344 None:intergenic 50.0%
! ATCGAGGATCTCAAGGCGTT+CGG - chr4.4:86285450-86285469 None:intergenic 50.0%
!! GGTGACAGGGTTGATGAAGA+GGG - chr4.4:86285183-86285202 None:intergenic 50.0%
!! TGGTGACAGGGTTGATGAAG+AGG - chr4.4:86285184-86285203 None:intergenic 50.0%
ACCAACCACCACGACCTCAA+CGG + chr4.4:86285146-86285165 MS.gene052795:CDS 55.0%
ACGCCTTGAGATCCTCGATG+AGG + chr4.4:86285451-86285470 MS.gene052795:CDS 55.0%
CAGCAGGGAGGCCAGATATA+AGG - chr4.4:86285418-86285437 None:intergenic 55.0%
GAACAGTCACCCTCTGTGGA+GGG - chr4.4:86285554-86285573 None:intergenic 55.0%
GACCGCCGAGCATAATTGGT+CGG - chr4.4:86285255-86285274 None:intergenic 55.0%
GGACTCAACTATCACCGTGC+CGG - chr4.4:86285576-86285595 None:intergenic 55.0%
TCCACAGAGGGTGACTGTTC+CGG + chr4.4:86285554-86285573 MS.gene052795:CDS 55.0%
TGACATGGCGATCCTCATCG+AGG - chr4.4:86285466-86285485 None:intergenic 55.0%
TGATGGAGGCGGTTGTCTCT+TGG - chr4.4:86285280-86285299 None:intergenic 55.0%
TGGTGTGGTTGATCCTGTGG+AGG - chr4.4:86285112-86285131 None:intergenic 55.0%
! TGATGGTCGTAGTGGTGGTG+TGG - chr4.4:86285127-86285146 None:intergenic 55.0%
! TGGTGGTGTGGTTGATCCTG+TGG - chr4.4:86285115-86285134 None:intergenic 55.0%
! TGTTTTCGCCGTTGAGGTCG+TGG - chr4.4:86285157-86285176 None:intergenic 55.0%
!! GTGGTTGGTGATGGTCGTAG+TGG - chr4.4:86285135-86285154 None:intergenic 55.0%
!! GTTGGTGATGGTCGTAGTGG+TGG - chr4.4:86285132-86285151 None:intergenic 55.0%
!! TGTAACCTCGTTGGTGGCGA+CGG + chr4.4:86285365-86285384 MS.gene052795:CDS 55.0%
!!! GGGTGTTGTTTTCGCCGTTG+AGG - chr4.4:86285163-86285182 None:intergenic 55.0%
ACGGTGATGTGGGCACACTC+AGG + chr4.4:86285384-86285403 MS.gene052795:CDS 60.0%
AGAGGGTGACTGTTCCGGCA+CGG + chr4.4:86285559-86285578 MS.gene052795:CDS 60.0%
CACTGACCAAACGGCGGTGA+TGG - chr4.4:86285297-86285316 None:intergenic 60.0%
CATCACCGCCGTTTGGTCAG+TGG + chr4.4:86285295-86285314 MS.gene052795:CDS 60.0%
CGGTGATGTGGGCACACTCA+GGG + chr4.4:86285385-86285404 MS.gene052795:CDS 60.0%
GCGAACCACTGACCAAACGG+CGG - chr4.4:86285303-86285322 None:intergenic 60.0%
GCGGCGAACCACTGACCAAA+CGG - chr4.4:86285306-86285325 None:intergenic 60.0%
GGAACAGTCACCCTCTGTGG+AGG - chr4.4:86285555-86285574 None:intergenic 60.0%
GGACGACCGCCGAGCATAAT+TGG - chr4.4:86285259-86285278 None:intergenic 60.0%
TGATGTGGGCACACTCAGGG+AGG + chr4.4:86285388-86285407 MS.gene052795:CDS 60.0%
TTTCGCCGTTGAGGTCGTGG+TGG - chr4.4:86285154-86285173 None:intergenic 60.0%
! TGACCAAACGGCGGTGATGG+AGG - chr4.4:86285294-86285313 None:intergenic 60.0%
!! GAGGTCGTGGTGGTTGGTGA+TGG - chr4.4:86285144-86285163 None:intergenic 60.0%
!! GTTGGTGGCGACGGTGATGT+GGG + chr4.4:86285374-86285393 MS.gene052795:CDS 60.0%
CATCACCGTCGCCACCAACG+AGG - chr4.4:86285373-86285392 None:intergenic 65.0%
GCCGGAACAGTCACCCTCTG+TGG - chr4.4:86285558-86285577 None:intergenic 65.0%
GCCGTTGAGGTCGTGGTGGT+TGG - chr4.4:86285150-86285169 None:intergenic 65.0%
!! CCAAACGGCGGTGATGGAGG+CGG - chr4.4:86285291-86285310 None:intergenic 65.0%
!! CGTTGGTGGCGACGGTGATG+TGG + chr4.4:86285373-86285392 MS.gene052795:CDS 65.0%
!! GTTCGGTGCTGCGTGCAGCA+GGG - chr4.4:86285433-86285452 None:intergenic 65.0%
CCGCCTCCATCACCGCCGTT+TGG + chr4.4:86285288-86285307 MS.gene052795:CDS 70.0%
!! CGTTCGGTGCTGCGTGCAGC+AGG - chr4.4:86285434-86285453 None:intergenic 70.0%
!! CGGTGCTGCGTGCAGCAGGG+AGG - chr4.4:86285430-86285449 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 86285077 86285733 86285077 ID=MS.gene052795
chr4.4 mRNA 86285077 86285733 86285077 ID=MS.gene052795.t1;Parent=MS.gene052795
chr4.4 exon 86285077 86285733 86285077 ID=MS.gene052795.t1.exon1;Parent=MS.gene052795.t1
chr4.4 CDS 86285077 86285733 86285077 ID=cds.MS.gene052795.t1;Parent=MS.gene052795.t1
Gene Sequence

>MS.gene052795

ATGCCTCCAAATCCACCAAAGTCTTCACTTCTCCTCCACAGGATCAACCACACCACCACTACGACCATCACCAACCACCACGACCTCAACGGCGAAAACAACACCCTCTTCATCAACCCTGTCACCAACACCATTCCTAATACTATAGCCCACTATCACACACACTCAATATCATCCGACCAATTATGCTCGGCGGTCGTCCAAGAGACAACCGCCTCCATCACCGCCGTTTGGTCAGTGGTTCGCCGCTTCGACAAACCACAAGCCTACAAAAATTTCATCAAGAGTTGTAACCTCGTTGGTGGCGACGGTGATGTGGGCACACTCAGGGAGGTCAACCTTATATCTGGCCTCCCTGCTGCACGCAGCACCGAACGCCTTGAGATCCTCGATGAGGATCGCCATGTCATCAGTTTTAGTGTTGTTGGTGGTGATCACAAGCTTGCTAACTACCGTTCCGTTACTACACTTCATCCCTCCACAGAGGGTGACTGTTCCGGCACGGTGATAGTTGAGTCCTATGTAGTGGATATTCCTTCGGAAAATACTAAAGAGGACACACATGTGTTTGTTGATACAATTGTTCGGTGTAACCTTCAGTCACTTGCACAAACCGCAGAGAACATTACACAGCAAAACAACAAGAGTTGTTCCTGA

Protein sequence

>MS.gene052795.t1

MPPNPPKSSLLLHRINHTTTTTITNHHDLNGENNTLFINPVTNTIPNTIAHYHTHSISSDQLCSAVVQETTASITAVWSVVRRFDKPQAYKNFIKSCNLVGGDGDVGTLREVNLISGLPAARSTERLEILDEDRHVISFSVVGGDHKLANYRSVTTLHPSTEGDCSGTVIVESYVVDIPSENTKEDTHVFVDTIVRCNLQSLAQTAENITQQNNKSCS