Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51081.t1 | XP_013470378.1 | 75.9 | 195 | 13 | 1 | 1 | 161 | 44 | 238 | 2.20E-70 | 275 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51081.t1 | Q39056 | 61.7 | 154 | 25 | 1 | 39 | 158 | 115 | 268 | 1.8e-41 | 170.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51081.t1 | B7FLR3 | 75.9 | 195 | 13 | 1 | 1 | 161 | 44 | 238 | 1.6e-70 | 275.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051710 | MS.gene51081 | 0.807435 | 5.06E-50 | -1.69E-46 |
| MS.gene058515 | MS.gene51081 | 0.815709 | 8.05E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51081.t1 | MTR_1g114590 | 75.897 | 195 | 13 | 1 | 1 | 161 | 44 | 238 | 3.19e-101 | 290 |
| MS.gene51081.t1 | MTR_5g015740 | 75.385 | 195 | 14 | 1 | 1 | 161 | 44 | 238 | 7.89e-100 | 287 |
| MS.gene51081.t1 | MTR_5g015730 | 92.308 | 39 | 3 | 0 | 123 | 161 | 60 | 98 | 9.27e-20 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51081.t1 | AT1G01290 | 61.688 | 154 | 25 | 1 | 39 | 158 | 115 | 268 | 9.44e-57 | 179 |
| MS.gene51081.t1 | AT1G01290 | 61.688 | 154 | 25 | 1 | 39 | 158 | 115 | 268 | 9.44e-57 | 179 |
Find 35 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAATGGAATCTGTATT+TGG | 0.170446 | 5.1:-65623411 | None:intergenic |
| GTTCTCTTGCTACTTTCTTT+TGG | 0.215531 | 5.1:+65623240 | None:intergenic |
| TTGACTCATACTGGAAATTC+AGG | 0.267783 | 5.1:-65623288 | MS.gene51081:CDS |
| AAGAAATGGAATCTGTATTT+GGG | 0.311719 | 5.1:-65623410 | None:intergenic |
| GACTTCAGTGTAACAATAGA+AGG | 0.351874 | 5.1:-65623018 | MS.gene51081:CDS |
| TCAAAATCATTATTGTTTCC+AGG | 0.353708 | 5.1:+65622831 | None:intergenic |
| CTCACAGTGGCGAAAATTGC+TGG | 0.376242 | 5.1:-65623135 | MS.gene51081:intron |
| ATTAGACTTAAGCATAAAAC+TGG | 0.405140 | 5.1:-65622874 | MS.gene51081:CDS |
| AATCCACTTGCACGTGTGTA+AGG | 0.416239 | 5.1:+65623058 | None:intergenic |
| ATGGCATCAGTGTCTATTGC+TGG | 0.423752 | 5.1:-65622952 | MS.gene51081:CDS |
| TGTTATGGCACAATGGAATC+AGG | 0.423838 | 5.1:+65623085 | None:intergenic |
| GAAGGGGAAGCTGCTTCAAC+CGG | 0.424889 | 5.1:-65623000 | MS.gene51081:CDS |
| CTATGTTTCGACATGACAAA+TGG | 0.441091 | 5.1:+65623328 | None:intergenic |
| TATGTTTCGACATGACAAAT+GGG | 0.465395 | 5.1:+65623329 | None:intergenic |
| ACAGAGCCAGCTAGTCCATC+TGG | 0.477776 | 5.1:+65623378 | None:intergenic |
| AAATTCAGGGGAAGCTCAAA+TGG | 0.480022 | 5.1:-65623274 | MS.gene51081:CDS |
| ATGTGCAAAGCTGCTTCAAA+AGG | 0.492586 | 5.1:-65622913 | MS.gene51081:CDS |
| TTTGGGGAACTTCCGCCAGA+TGG | 0.539226 | 5.1:-65623393 | MS.gene51081:CDS |
| CTTCCGCCAGATGGACTAGC+TGG | 0.541756 | 5.1:-65623384 | MS.gene51081:CDS |
| ATTCCATTGTGCCATAACAT+AGG | 0.562881 | 5.1:-65623081 | MS.gene51081:CDS |
| CATTGCTTCCATCTCAACCC+CGG | 0.567098 | 5.1:+65622972 | None:intergenic |
| CATTTGTCATGTCGAAACAT+AGG | 0.568946 | 5.1:-65623327 | MS.gene51081:CDS |
| ACTTCAGTGTAACAATAGAA+GGG | 0.575457 | 5.1:-65623017 | MS.gene51081:CDS |
| AGGCCTTACACACGTGCAAG+TGG | 0.575488 | 5.1:-65623061 | MS.gene51081:CDS |
| CGGGGTTGAGATGGAAGCAA+TGG | 0.576890 | 5.1:-65622971 | MS.gene51081:CDS |
| CGTGTGTAAGGCCTATGTTA+TGG | 0.577316 | 5.1:+65623070 | None:intergenic |
| TGACTCATACTGGAAATTCA+GGG | 0.590004 | 5.1:-65623287 | MS.gene51081:CDS |
| AGACTTAAGCATAAAACTGG+TGG | 0.628630 | 5.1:-65622871 | MS.gene51081:CDS |
| CTTCAGTGTAACAATAGAAG+GGG | 0.630495 | 5.1:-65623016 | MS.gene51081:CDS |
| GACTCATACTGGAAATTCAG+GGG | 0.640275 | 5.1:-65623286 | MS.gene51081:CDS |
| GGATTTGAGATTGAATCATG+AGG | 0.642472 | 5.1:-65623040 | MS.gene51081:CDS |
| AGAAATGGAATCTGTATTTG+GGG | 0.645520 | 5.1:-65623409 | None:intergenic |
| AGGCCTATGTTATGGCACAA+TGG | 0.661858 | 5.1:+65623078 | None:intergenic |
| ATGTTTCGACATGACAAATG+GGG | 0.681732 | 5.1:+65623330 | None:intergenic |
| GAGCCAGCTAGTCCATCTGG+CGG | 0.697985 | 5.1:+65623381 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTAGACTTAAGCATAAAAC+TGG | - | chr5.1:65623371-65623390 | MS.gene51081:CDS | 25.0% |
| ACTTCAGTGTAACAATAGAA+GGG | - | chr5.1:65623228-65623247 | MS.gene51081:intron | 30.0% | |
| ATAAAACTGGTGGAAAAAGT+GGG | - | chr5.1:65623384-65623403 | MS.gene51081:CDS | 30.0% | |
| CAAAGTAAATCTTGGAAAGA+AGG | - | chr5.1:65623043-65623062 | MS.gene51081:CDS | 30.0% | |
| TATGTTTCGACATGACAAAT+GGG | + | chr5.1:65622919-65622938 | None:intergenic | 30.0% | |
| TTTGGTATTAGCCAATCAAA+TGG | - | chr5.1:65623073-65623092 | MS.gene51081:CDS | 30.0% | |
| !! | GTCTCATTTTTCCATTTGAT+TGG | + | chr5.1:65623087-65623106 | None:intergenic | 30.0% |
| !! | TCTTTTGAATTGACTCATAC+TGG | - | chr5.1:65622948-65622967 | MS.gene51081:CDS | 30.0% |
| AGACTTAAGCATAAAACTGG+TGG | - | chr5.1:65623374-65623393 | MS.gene51081:CDS | 35.0% | |
| ATGTTTCGACATGACAAATG+GGG | + | chr5.1:65622918-65622937 | None:intergenic | 35.0% | |
| ATTCCATTGTGCCATAACAT+AGG | - | chr5.1:65623164-65623183 | MS.gene51081:intron | 35.0% | |
| ATTTACTTTGCAAACAGCAG+TGG | + | chr5.1:65623033-65623052 | None:intergenic | 35.0% | |
| CATAAAACTGGTGGAAAAAG+TGG | - | chr5.1:65623383-65623402 | MS.gene51081:CDS | 35.0% | |
| CATTTGTCATGTCGAAACAT+AGG | - | chr5.1:65622918-65622937 | MS.gene51081:CDS | 35.0% | |
| CTATGTTTCGACATGACAAA+TGG | + | chr5.1:65622920-65622939 | None:intergenic | 35.0% | |
| CTTCAGTGTAACAATAGAAG+GGG | - | chr5.1:65623229-65623248 | MS.gene51081:intron | 35.0% | |
| GACTTCAGTGTAACAATAGA+AGG | - | chr5.1:65623227-65623246 | MS.gene51081:intron | 35.0% | |
| GCTGTTTGCAAAGTAAATCT+TGG | - | chr5.1:65623035-65623054 | MS.gene51081:CDS | 35.0% | |
| TAAAACTGGTGGAAAAAGTG+GGG | - | chr5.1:65623385-65623404 | MS.gene51081:CDS | 35.0% | |
| TGACTCATACTGGAAATTCA+GGG | - | chr5.1:65622958-65622977 | MS.gene51081:CDS | 35.0% | |
| TTGACTCATACTGGAAATTC+AGG | - | chr5.1:65622957-65622976 | MS.gene51081:CDS | 35.0% | |
| ! | GGATTTGAGATTGAATCATG+AGG | - | chr5.1:65623205-65623224 | MS.gene51081:intron | 35.0% |
| !! | TGGAAAGAAGGTGTTTGATT+TGG | - | chr5.1:65623055-65623074 | MS.gene51081:CDS | 35.0% |
| !!! | GTTCTCTTGCTACTTTCTTT+TGG | + | chr5.1:65623008-65623027 | None:intergenic | 35.0% |
| AAATTCAGGGGAAGCTCAAA+TGG | - | chr5.1:65622971-65622990 | MS.gene51081:CDS | 40.0% | |
| ATGTGCAAAGCTGCTTCAAA+AGG | - | chr5.1:65623332-65623351 | MS.gene51081:CDS | 40.0% | |
| GACTCATACTGGAAATTCAG+GGG | - | chr5.1:65622959-65622978 | MS.gene51081:CDS | 40.0% | |
| TGTTATGGCACAATGGAATC+AGG | + | chr5.1:65623163-65623182 | None:intergenic | 40.0% | |
| AAAATGAGACGTGCTCACAG+TGG | - | chr5.1:65623097-65623116 | MS.gene51081:CDS | 45.0% | |
| AATCCACTTGCACGTGTGTA+AGG | + | chr5.1:65623190-65623209 | None:intergenic | 45.0% | |
| AGGCCTATGTTATGGCACAA+TGG | + | chr5.1:65623170-65623189 | None:intergenic | 45.0% | |
| ATGGCATCAGTGTCTATTGC+TGG | - | chr5.1:65623293-65623312 | MS.gene51081:CDS | 45.0% | |
| CGTGTGTAAGGCCTATGTTA+TGG | + | chr5.1:65623178-65623197 | None:intergenic | 45.0% | |
| GAAAAAGTGGGGATTACTCC+TGG | - | chr5.1:65623396-65623415 | MS.gene51081:CDS | 45.0% | |
| CATTGCTTCCATCTCAACCC+CGG | + | chr5.1:65623276-65623295 | None:intergenic | 50.0% | |
| CTCACAGTGGCGAAAATTGC+TGG | - | chr5.1:65623110-65623129 | MS.gene51081:CDS | 50.0% | |
| CTGCTTCAACCGGAAAAACC+GGG | - | chr5.1:65623255-65623274 | MS.gene51081:CDS | 50.0% | |
| GCTGCTTCAACCGGAAAAAC+CGG | - | chr5.1:65623254-65623273 | MS.gene51081:CDS | 50.0% | |
| TGCTTCAACCGGAAAAACCG+GGG | - | chr5.1:65623256-65623275 | MS.gene51081:CDS | 50.0% | |
| ! | CATCTCAACCCCGGTTTTTC+CGG | + | chr5.1:65623267-65623286 | None:intergenic | 50.0% |
| ACAGAGCCAGCTAGTCCATC+TGG | + | chr5.1:65622870-65622889 | None:intergenic | 55.0% | |
| AGGCCTTACACACGTGCAAG+TGG | - | chr5.1:65623184-65623203 | MS.gene51081:intron | 55.0% | |
| CGGAAAAACCGGGGTTGAGA+TGG | - | chr5.1:65623265-65623284 | MS.gene51081:CDS | 55.0% | |
| CGGGGTTGAGATGGAAGCAA+TGG | - | chr5.1:65623274-65623293 | MS.gene51081:CDS | 55.0% | |
| GAAGGGGAAGCTGCTTCAAC+CGG | - | chr5.1:65623245-65623264 | MS.gene51081:intron | 55.0% | |
| TTTGGGGAACTTCCGCCAGA+TGG | - | chr5.1:65622852-65622871 | MS.gene51081:CDS | 55.0% | |
| GAGCCAGCTAGTCCATCTGG+CGG | + | chr5.1:65622867-65622886 | None:intergenic | 60.0% | |
| !! | CTTCCGCCAGATGGACTAGC+TGG | - | chr5.1:65622861-65622880 | MS.gene51081:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 65622840 | 65623427 | 65622840 | ID=MS.gene51081 |
| chr5.1 | mRNA | 65622840 | 65623427 | 65622840 | ID=MS.gene51081.t1;Parent=MS.gene51081 |
| chr5.1 | exon | 65623253 | 65623427 | 65623253 | ID=MS.gene51081.t1.exon1;Parent=MS.gene51081.t1 |
| chr5.1 | CDS | 65623253 | 65623427 | 65623253 | ID=cds.MS.gene51081.t1;Parent=MS.gene51081.t1 |
| chr5.1 | exon | 65622840 | 65623150 | 65622840 | ID=MS.gene51081.t1.exon2;Parent=MS.gene51081.t1 |
| chr5.1 | CDS | 65622840 | 65623150 | 65622840 | ID=cds.MS.gene51081.t1;Parent=MS.gene51081.t1 |
| Gene Sequence |
| Protein sequence |
