Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51099.t1 | XP_003616732.1 | 99.3 | 134 | 1 | 0 | 1 | 134 | 1 | 134 | 1.10E-72 | 282.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51099.t1 | Q39096 | 35.9 | 156 | 66 | 6 | 12 | 134 | 9 | 163 | 2.5e-12 | 73.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51099.t1 | G7K7B0 | 99.3 | 134 | 1 | 0 | 1 | 134 | 1 | 134 | 8.2e-73 | 282.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053562 | MS.gene51099 | 0.804685 | 1.92E-49 | -1.69E-46 |
| MS.gene053633 | MS.gene51099 | -0.801719 | 7.89E-49 | -1.69E-46 |
| MS.gene057173 | MS.gene51099 | 0.833826 | 4.27E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51099.t1 | MTR_5g083690 | 99.254 | 134 | 1 | 0 | 1 | 134 | 1 | 134 | 7.34e-98 | 277 |
| MS.gene51099.t1 | MTR_3g023110 | 32.544 | 169 | 76 | 3 | 4 | 134 | 1 | 169 | 6.97e-21 | 83.2 |
| MS.gene51099.t1 | MTR_7g090630 | 44.595 | 74 | 37 | 2 | 1 | 73 | 1 | 71 | 1.06e-15 | 68.2 |
| MS.gene51099.t1 | MTR_7g090630 | 44.595 | 74 | 37 | 2 | 1 | 73 | 1 | 71 | 1.18e-15 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51099.t1 | AT2G41430 | 31.737 | 167 | 77 | 5 | 1 | 134 | 1 | 163 | 2.90e-15 | 68.6 |
| MS.gene51099.t1 | AT2G41430 | 31.737 | 167 | 77 | 5 | 1 | 134 | 1 | 163 | 2.90e-15 | 68.6 |
| MS.gene51099.t1 | AT2G41430 | 31.737 | 167 | 77 | 5 | 1 | 134 | 1 | 163 | 2.90e-15 | 68.6 |
| MS.gene51099.t1 | AT2G41430 | 31.737 | 167 | 77 | 5 | 1 | 134 | 1 | 163 | 2.90e-15 | 68.6 |
| MS.gene51099.t1 | AT4G14270 | 50.000 | 66 | 25 | 2 | 12 | 75 | 10 | 69 | 2.36e-14 | 65.9 |
| MS.gene51099.t1 | AT4G14270 | 50.000 | 66 | 25 | 2 | 12 | 75 | 10 | 69 | 2.36e-14 | 65.9 |
| MS.gene51099.t1 | AT2G41430 | 44.615 | 65 | 33 | 1 | 1 | 65 | 1 | 62 | 2.86e-14 | 65.1 |
Find 45 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCCTTGGTTCCGTGATTAC+TGG | 0.164334 | 5.1:+65867001 | MS.gene51099:CDS |
| TGGGAAAGAGTTGATGAAAT+TGG | 0.243769 | 5.1:+65867339 | MS.gene51099:CDS |
| ATTATGCCACCAAATCTATC+TGG | 0.305386 | 5.1:-65867470 | None:intergenic |
| ACCTCGTTCATTCCTCCCCT+TGG | 0.371694 | 5.1:+65866986 | MS.gene51099:CDS |
| GGGAAAGAGTTGATGAAATT+GGG | 0.389970 | 5.1:+65867340 | MS.gene51099:CDS |
| TTTCATAGTTCTGAGGATCT+TGG | 0.395345 | 5.1:-65867038 | None:intergenic |
| GAGGATCTTGGAAGCGTTCA+CGG | 0.427509 | 5.1:-65867026 | None:intergenic |
| AATAGCCCGTGGGCTCACTC+TGG | 0.435925 | 5.1:-65867440 | None:intergenic |
| TGAAATTGGGATCGTTGAAA+TGG | 0.456391 | 5.1:+65867353 | MS.gene51099:CDS |
| ACTCTGGGCTTCACAATCTT+CGG | 0.460415 | 5.1:-65867424 | None:intergenic |
| GCGCGTGTAGAACACGAAGA+TGG | 0.465797 | 5.1:+65867319 | MS.gene51099:intron |
| CGCGTGTAGAACACGAAGAT+GGG | 0.474343 | 5.1:+65867320 | MS.gene51099:intron |
| GGAACGAACATCGGAGCGTT+GGG | 0.476212 | 5.1:-65866919 | None:intergenic |
| CGGAACGAACATCGGAGCGT+TGG | 0.492883 | 5.1:-65866920 | None:intergenic |
| GGGGTTCAATTTCATGAACA+TGG | 0.523834 | 5.1:-65867107 | None:intergenic |
| GGAGCCTTCTCAGCATACCT+TGG | 0.524540 | 5.1:-65867403 | None:intergenic |
| GTTGGGATTCAGCGAGGAGG+AGG | 0.529767 | 5.1:-65866902 | None:intergenic |
| TGGAGGATTTCTCCGATGAA+TGG | 0.530316 | 5.1:+65866959 | MS.gene51099:CDS |
| ATAGCCCGTGGGCTCACTCT+GGG | 0.531409 | 5.1:-65867439 | None:intergenic |
| TTATGCCACCAAATCTATCT+GGG | 0.545633 | 5.1:-65867469 | None:intergenic |
| GCCAGTAATCACGGAACCAA+GGG | 0.550209 | 5.1:-65867002 | None:intergenic |
| AGTTCCAAGGTATGCTGAGA+AGG | 0.558393 | 5.1:+65867399 | MS.gene51099:CDS |
| AACGAGGTTCCACCATTCAT+CGG | 0.566948 | 5.1:-65866971 | None:intergenic |
| CTGGGCTGATGAATAGCCCG+TGG | 0.567215 | 5.1:-65867451 | None:intergenic |
| TGAAGCCCAGAGTGAGCCCA+CGG | 0.571247 | 5.1:+65867434 | MS.gene51099:CDS |
| TTGAAATGGAGAGGATCTGA+TGG | 0.578122 | 5.1:+65867367 | MS.gene51099:CDS |
| TACGCAAGCGGAACGAACAT+CGG | 0.582960 | 5.1:-65866928 | None:intergenic |
| CGTTCCGCTTGCGTATCGTA+CGG | 0.583236 | 5.1:+65866936 | MS.gene51099:CDS |
| ATGGATGGGCTGAAGTTCCA+AGG | 0.594708 | 5.1:+65867386 | MS.gene51099:CDS |
| GCTTGCGTATCGTACGGTGG+AGG | 0.600361 | 5.1:+65866942 | MS.gene51099:CDS |
| TTGGGATCGTTGAAATGGAG+AGG | 0.600872 | 5.1:+65867358 | MS.gene51099:CDS |
| AGCCAGTAATCACGGAACCA+AGG | 0.611593 | 5.1:-65867003 | None:intergenic |
| AGGATTTCTCCGATGAATGG+TGG | 0.617511 | 5.1:+65866962 | MS.gene51099:CDS |
| AATGGAGAGGATCTGATGGA+TGG | 0.618481 | 5.1:+65867371 | MS.gene51099:CDS |
| ATGGAGAGGATCTGATGGAT+GGG | 0.622633 | 5.1:+65867372 | MS.gene51099:CDS |
| GAAGCCCAGAGTGAGCCCAC+GGG | 0.627082 | 5.1:+65867435 | MS.gene51099:CDS |
| GTTCACGGAGCCAGTAATCA+CGG | 0.629661 | 5.1:-65867011 | None:intergenic |
| TGGGCTGATGAATAGCCCGT+GGG | 0.658555 | 5.1:-65867450 | None:intergenic |
| TCCACCGTACGATACGCAAG+CGG | 0.663483 | 5.1:-65866940 | None:intergenic |
| TCCGCTTGCGTATCGTACGG+TGG | 0.666184 | 5.1:+65866939 | MS.gene51099:CDS |
| AGCGTTGGGATTCAGCGAGG+AGG | 0.680838 | 5.1:-65866905 | None:intergenic |
| GTAATCACGGAACCAAGGGG+AGG | 0.688535 | 5.1:-65866998 | None:intergenic |
| CCAGTAATCACGGAACCAAG+GGG | 0.718986 | 5.1:-65867001 | None:intergenic |
| ACCAAGGGGAGGAATGAACG+AGG | 0.740764 | 5.1:-65866987 | None:intergenic |
| CGGAGCGTTGGGATTCAGCG+AGG | 0.742236 | 5.1:-65866908 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTATGTGATTAATTTTGA+TGG | + | chr5.1:65867271-65867290 | MS.gene51099:intron | 15.0% |
| !! | AATAAAGTAAAATAGCTTAC+GGG | - | chr5.1:65867130-65867149 | None:intergenic | 20.0% |
| !! | CAATAAAGTAAAATAGCTTA+CGG | - | chr5.1:65867131-65867150 | None:intergenic | 20.0% |
| !!! | GATTAATTTTGATGGATTAA+TGG | + | chr5.1:65867279-65867298 | MS.gene51099:intron | 20.0% |
| ! | ATAAAGTAAAATAGCTTACG+GGG | - | chr5.1:65867129-65867148 | None:intergenic | 25.0% |
| !!! | GTTTTCTGATTTTGAAATGA+TGG | + | chr5.1:65867068-65867087 | MS.gene51099:CDS | 25.0% |
| ! | AATGGATCTGATGTTTGTTT+TGG | + | chr5.1:65867297-65867316 | MS.gene51099:intron | 30.0% |
| TGAAATTGGGATCGTTGAAA+TGG | + | chr5.1:65867353-65867372 | MS.gene51099:CDS | 35.0% | |
| !! | GAGTCATTTTCATAGTTCTG+AGG | - | chr5.1:65867048-65867067 | None:intergenic | 35.0% |
| !! | GGGAAAGAGTTGATGAAATT+GGG | + | chr5.1:65867340-65867359 | MS.gene51099:CDS | 35.0% |
| !! | TGGGAAAGAGTTGATGAAAT+TGG | + | chr5.1:65867339-65867358 | MS.gene51099:CDS | 35.0% |
| !! | TTTCATAGTTCTGAGGATCT+TGG | - | chr5.1:65867041-65867060 | None:intergenic | 35.0% |
| GGGGTTCAATTTCATGAACA+TGG | - | chr5.1:65867110-65867129 | None:intergenic | 40.0% | |
| TTGAAATGGAGAGGATCTGA+TGG | + | chr5.1:65867367-65867386 | MS.gene51099:CDS | 40.0% | |
| AACGAGGTTCCACCATTCAT+CGG | - | chr5.1:65866974-65866993 | None:intergenic | 45.0% | |
| AATGGAGAGGATCTGATGGA+TGG | + | chr5.1:65867371-65867390 | MS.gene51099:CDS | 45.0% | |
| ACTCTGGGCTTCACAATCTT+CGG | - | chr5.1:65867427-65867446 | None:intergenic | 45.0% | |
| AGGATTTCTCCGATGAATGG+TGG | + | chr5.1:65866962-65866981 | MS.gene51099:CDS | 45.0% | |
| AGTTCCAAGGTATGCTGAGA+AGG | + | chr5.1:65867399-65867418 | MS.gene51099:CDS | 45.0% | |
| TGGAGGATTTCTCCGATGAA+TGG | + | chr5.1:65866959-65866978 | MS.gene51099:CDS | 45.0% | |
| TTGGGATCGTTGAAATGGAG+AGG | + | chr5.1:65867358-65867377 | MS.gene51099:CDS | 45.0% | |
| ! | ATGGAGAGGATCTGATGGAT+GGG | + | chr5.1:65867372-65867391 | MS.gene51099:CDS | 45.0% |
| AGCCAGTAATCACGGAACCA+AGG | - | chr5.1:65867006-65867025 | None:intergenic | 50.0% | |
| CCAGTAATCACGGAACCAAG+GGG | - | chr5.1:65867004-65867023 | None:intergenic | 50.0% | |
| CGCGTGTAGAACACGAAGAT+GGG | + | chr5.1:65867320-65867339 | MS.gene51099:intron | 50.0% | |
| GCCAGTAATCACGGAACCAA+GGG | - | chr5.1:65867005-65867024 | None:intergenic | 50.0% | |
| GTTCACGGAGCCAGTAATCA+CGG | - | chr5.1:65867014-65867033 | None:intergenic | 50.0% | |
| TACGCAAGCGGAACGAACAT+CGG | - | chr5.1:65866931-65866950 | None:intergenic | 50.0% | |
| !! | ATGGATGGGCTGAAGTTCCA+AGG | + | chr5.1:65867386-65867405 | MS.gene51099:CDS | 50.0% |
| !! | GAGGATCTTGGAAGCGTTCA+CGG | - | chr5.1:65867029-65867048 | None:intergenic | 50.0% |
| ACCAAGGGGAGGAATGAACG+AGG | - | chr5.1:65866990-65867009 | None:intergenic | 55.0% | |
| ACCTCGTTCATTCCTCCCCT+TGG | + | chr5.1:65866986-65867005 | MS.gene51099:CDS | 55.0% | |
| CCCCTTGGTTCCGTGATTAC+TGG | + | chr5.1:65867001-65867020 | MS.gene51099:CDS | 55.0% | |
| CGTTCCGCTTGCGTATCGTA+CGG | + | chr5.1:65866936-65866955 | MS.gene51099:CDS | 55.0% | |
| GCGCGTGTAGAACACGAAGA+TGG | + | chr5.1:65867319-65867338 | MS.gene51099:intron | 55.0% | |
| GGAGCCTTCTCAGCATACCT+TGG | - | chr5.1:65867406-65867425 | None:intergenic | 55.0% | |
| GTAATCACGGAACCAAGGGG+AGG | - | chr5.1:65867001-65867020 | None:intergenic | 55.0% | |
| TCCACCGTACGATACGCAAG+CGG | - | chr5.1:65866943-65866962 | None:intergenic | 55.0% | |
| TGGGCTGATGAATAGCCCGT+GGG | - | chr5.1:65867453-65867472 | None:intergenic | 55.0% | |
| ! | GGAACGAACATCGGAGCGTT+GGG | - | chr5.1:65866922-65866941 | None:intergenic | 55.0% |
| AATAGCCCGTGGGCTCACTC+TGG | - | chr5.1:65867443-65867462 | None:intergenic | 60.0% | |
| ATAGCCCGTGGGCTCACTCT+GGG | - | chr5.1:65867442-65867461 | None:intergenic | 60.0% | |
| CGGAACGAACATCGGAGCGT+TGG | - | chr5.1:65866923-65866942 | None:intergenic | 60.0% | |
| CTGGGCTGATGAATAGCCCG+TGG | - | chr5.1:65867454-65867473 | None:intergenic | 60.0% | |
| GCTTGCGTATCGTACGGTGG+AGG | + | chr5.1:65866942-65866961 | MS.gene51099:CDS | 60.0% | |
| GTTGGGATTCAGCGAGGAGG+AGG | - | chr5.1:65866905-65866924 | None:intergenic | 60.0% | |
| TCCGCTTGCGTATCGTACGG+TGG | + | chr5.1:65866939-65866958 | MS.gene51099:CDS | 60.0% | |
| TGAAGCCCAGAGTGAGCCCA+CGG | + | chr5.1:65867434-65867453 | MS.gene51099:CDS | 60.0% | |
| ! | AGCGTTGGGATTCAGCGAGG+AGG | - | chr5.1:65866908-65866927 | None:intergenic | 60.0% |
| GAAGCCCAGAGTGAGCCCAC+GGG | + | chr5.1:65867435-65867454 | MS.gene51099:CDS | 65.0% | |
| !! | CGGAGCGTTGGGATTCAGCG+AGG | - | chr5.1:65866911-65866930 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 65866874 | 65867477 | 65866874 | ID=MS.gene51099 |
| chr5.1 | mRNA | 65866874 | 65867477 | 65866874 | ID=MS.gene51099.t1;Parent=MS.gene51099 |
| chr5.1 | exon | 65866874 | 65867129 | 65866874 | ID=MS.gene51099.t1.exon1;Parent=MS.gene51099.t1 |
| chr5.1 | CDS | 65866874 | 65867129 | 65866874 | ID=cds.MS.gene51099.t1;Parent=MS.gene51099.t1 |
| chr5.1 | exon | 65867329 | 65867477 | 65867329 | ID=MS.gene51099.t1.exon2;Parent=MS.gene51099.t1 |
| chr5.1 | CDS | 65867329 | 65867477 | 65867329 | ID=cds.MS.gene51099.t1;Parent=MS.gene51099.t1 |
| Gene Sequence |
| Protein sequence |
