Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51169.t1 | XP_003624160.1 | 97.7 | 172 | 3 | 1 | 1 | 171 | 1 | 172 | 1.60E-87 | 332 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51169.t1 | Q9FFD5 | 44.8 | 165 | 71 | 6 | 1 | 163 | 1 | 147 | 8.2e-24 | 111.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51169.t1 | Q2HW10 | 97.7 | 172 | 3 | 1 | 1 | 171 | 1 | 172 | 1.2e-87 | 332.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058722 | MS.gene51169 | 0.981521 | 8.58E-153 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51169.t1 | MTR_7g079870 | 97.674 | 172 | 3 | 1 | 1 | 171 | 1 | 172 | 4.39e-122 | 341 |
| MS.gene51169.t1 | MTR_7g021660 | 41.139 | 158 | 79 | 5 | 1 | 156 | 1 | 146 | 2.49e-28 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51169.t1 | AT5G16490 | 48.052 | 154 | 64 | 6 | 1 | 152 | 1 | 140 | 6.67e-31 | 109 |
| MS.gene51169.t1 | AT1G27380 | 34.416 | 154 | 69 | 4 | 1 | 129 | 1 | 147 | 2.05e-16 | 72.8 |
Find 42 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGGAGATGTTTCATGTTT+TGG | 0.253272 | 7.3:+27466039 | None:intergenic |
| GATGAAATGGACATGGAAAT+AGG | 0.297095 | 7.3:-27466201 | MS.gene51169:CDS |
| GTCATTCTTCCTTTCTCAGC+TGG | 0.335866 | 7.3:-27467754 | MS.gene51169:CDS |
| TTGAAGGATAGGTTAGGCTT+TGG | 0.378556 | 7.3:+27466859 | None:intergenic |
| GGACTTAGAAGAAGATGAAA+TGG | 0.383098 | 7.3:-27466214 | MS.gene51169:CDS |
| AGAGAATCCCATCCCCTCAT+TGG | 0.414199 | 7.3:+27466105 | None:intergenic |
| TCAATTAATCAAGCAATAGA+AGG | 0.417530 | 7.3:+27466006 | None:intergenic |
| GCCTTTGAACAACTTGTTGA+AGG | 0.426359 | 7.3:+27466843 | None:intergenic |
| ATGAAATGGACATGGAAATA+GGG | 0.448347 | 7.3:-27466200 | MS.gene51169:CDS |
| GAGGATTCAGAGACTTTGTC+TGG | 0.450700 | 7.3:-27466922 | MS.gene51169:CDS |
| AATAGAAGGCATGATGTTGA+TGG | 0.460773 | 7.3:+27466020 | None:intergenic |
| AACATGTGACACACATTGGT+TGG | 0.460995 | 7.3:-27466161 | MS.gene51169:CDS |
| TGAACAACTTGTTGAAGGAT+AGG | 0.476710 | 7.3:+27466848 | None:intergenic |
| TGTGGTGAATTAGTCTCTGC+AGG | 0.479443 | 7.3:+27467676 | None:intergenic |
| GAGAATCCCATCCCCTCATT+GGG | 0.490983 | 7.3:+27466106 | None:intergenic |
| ATAAGATTAGAAGGATCTAA+AGG | 0.496998 | 7.3:-27466949 | MS.gene51169:intron |
| CTCATGACCCAATGAGGGGA+TGG | 0.511835 | 7.3:-27466113 | MS.gene51169:CDS |
| GAGGCTACAGAGAGAAGCTC+AGG | 0.512955 | 7.3:+27466075 | None:intergenic |
| AGAAAGTATGAGACAGAGAA+TGG | 0.521813 | 7.3:-27467785 | None:intergenic |
| TCCTTCAACAAGTTGTTCAA+AGG | 0.523718 | 7.3:-27466844 | MS.gene51169:CDS |
| GTCACATGTTGTACATCTGT+TGG | 0.530235 | 7.3:+27466174 | None:intergenic |
| TCACATGTTGTACATCTGTT+GGG | 0.533947 | 7.3:+27466175 | None:intergenic |
| GAGATACAACCAGCTGAGAA+AGG | 0.537814 | 7.3:+27467745 | None:intergenic |
| TCTGAGGCTAGTGTTGCTGT+TGG | 0.544954 | 7.3:-27467724 | MS.gene51169:CDS |
| AAAGTATGAAGAATTCTTTG+AGG | 0.549357 | 7.3:-27466897 | MS.gene51169:CDS |
| ACATGTGACACACATTGGTT+GGG | 0.559436 | 7.3:-27466160 | MS.gene51169:CDS |
| TCATGACCCAATGAGGGGAT+GGG | 0.567190 | 7.3:-27466112 | MS.gene51169:CDS |
| CTCAGCTGGTTGTATCTCTG+AGG | 0.578716 | 7.3:-27467740 | MS.gene51169:CDS |
| ATAGAAGGCATGATGTTGAT+GGG | 0.591363 | 7.3:+27466021 | None:intergenic |
| AACTTGTTGAAGGATAGGTT+AGG | 0.593453 | 7.3:+27466853 | None:intergenic |
| GCTACAGAGAGAAGCTCAGG+AGG | 0.604067 | 7.3:+27466078 | None:intergenic |
| AATTGCAGTGGAGAAAGAAG+AGG | 0.604469 | 7.3:-27466235 | MS.gene51169:intron |
| TGGGTCATGAGCAGCACATG+AGG | 0.609467 | 7.3:+27466125 | None:intergenic |
| GTGACACACATTGGTTGGGA+TGG | 0.614285 | 7.3:-27466156 | MS.gene51169:CDS |
| TGCTGCTCATGACCCAATGA+GGG | 0.614602 | 7.3:-27466118 | MS.gene51169:CDS |
| GTATGAGACAGAGAATGGAA+AGG | 0.630032 | 7.3:-27467780 | MS.gene51169:CDS |
| AGAAGAAGATGAAATGGACA+TGG | 0.630572 | 7.3:-27466208 | MS.gene51169:CDS |
| AGAAGGATCTAAAGGAGTCG+AGG | 0.632435 | 7.3:-27466941 | MS.gene51169:CDS |
| AAGAGACTTATTGCATCATG+TGG | 0.639239 | 7.3:+27467658 | None:intergenic |
| GTGCTGCTCATGACCCAATG+AGG | 0.658635 | 7.3:-27466119 | MS.gene51169:CDS |
| GCTGCTCATGACCCAATGAG+GGG | 0.751010 | 7.3:-27466117 | MS.gene51169:CDS |
| GTACAACATGTGACACACAT+TGG | 0.753481 | 7.3:-27466165 | MS.gene51169:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTTGTAAATAAAAATATA+TGG | - | chr7.3:27467490-27467509 | MS.gene51169:intron | 10.0% |
| !!! | ATTCTAAAAATGTTTATTTA+GGG | + | chr7.3:27467167-27467186 | None:intergenic | 10.0% |
| !!! | CTTGTATTTTAAATTTTATT+TGG | - | chr7.3:27466511-27466530 | MS.gene51169:intron | 10.0% |
| !! | AATATATGTTTAATAGAGAA+TGG | + | chr7.3:27466609-27466628 | None:intergenic | 15.0% |
| !! | CATAATAAAAATAAAACTGT+AGG | - | chr7.3:27467443-27467462 | MS.gene51169:intron | 15.0% |
| !!! | CATTCTAAAAATGTTTATTT+AGG | + | chr7.3:27467168-27467187 | None:intergenic | 15.0% |
| !!! | TTCTCTAATTTTTAATTAGT+TGG | - | chr7.3:27466750-27466769 | MS.gene51169:intron | 15.0% |
| !!! | TTTTTCTTTTTTGAAAGTTA+CGG | + | chr7.3:27466719-27466738 | None:intergenic | 15.0% |
| !! | AAATTTATGTTTAATTGCAG+TGG | - | chr7.3:27467545-27467564 | MS.gene51169:intron | 20.0% |
| !! | ATGTATAAAAAAAAATGTCG+TGG | - | chr7.3:27466663-27466682 | MS.gene51169:intron | 20.0% |
| !! | TACTAGCTAAATAAAACTAA+GGG | + | chr7.3:27466241-27466260 | None:intergenic | 20.0% |
| !! | TGTATAAAAAAAAATGTCGT+GGG | - | chr7.3:27466664-27466683 | MS.gene51169:intron | 20.0% |
| !! | TTACTAGCTAAATAAAACTA+AGG | + | chr7.3:27466242-27466261 | None:intergenic | 20.0% |
| !!! | ATAAAATTGGGTTTAAGATA+AGG | + | chr7.3:27467310-27467329 | None:intergenic | 20.0% |
| ! | AAAGTATGAAGAATTCTTTG+AGG | - | chr7.3:27466895-27466914 | MS.gene51169:CDS | 25.0% |
| ! | ATAAGATTAGAAGGATCTAA+AGG | - | chr7.3:27466843-27466862 | MS.gene51169:CDS | 25.0% |
| ! | ATACAATAAATTTGACCATC+GGG | + | chr7.3:27466566-27466585 | None:intergenic | 25.0% |
| ! | CATACAATAAATTTGACCAT+CGG | + | chr7.3:27466567-27466586 | None:intergenic | 25.0% |
| ! | GAACGAAATAAAAACAAACA+AGG | + | chr7.3:27467105-27467124 | None:intergenic | 25.0% |
| ! | GATATCATAGTAAAAGAGAT+AGG | + | chr7.3:27466160-27466179 | None:intergenic | 25.0% |
| ! | GATGATATGATAAGATTAGA+AGG | - | chr7.3:27466834-27466853 | MS.gene51169:CDS | 25.0% |
| ! | GTATAAAAAAAAATGTCGTG+GGG | - | chr7.3:27466665-27466684 | MS.gene51169:intron | 25.0% |
| ! | TGAATTTAGTCATTCGATAA+AGG | - | chr7.3:27466326-27466345 | MS.gene51169:intron | 25.0% |
| ! | TGTTTAATAGAGAATGGAAA+AGG | + | chr7.3:27466603-27466622 | None:intergenic | 25.0% |
| !! | AAGAACTTTTCTCAATTGTT+TGG | - | chr7.3:27466975-27466994 | MS.gene51169:intron | 25.0% |
| !! | ATGGAAGTCTTAATTTTTCA+TGG | - | chr7.3:27467509-27467528 | MS.gene51169:intron | 25.0% |
| !! | GTTTTCTCATACTAATTTTG+TGG | - | chr7.3:27467016-27467035 | MS.gene51169:intron | 25.0% |
| !! | TAAACCCAATTTTATAGAGT+CGG | - | chr7.3:27467315-27467334 | MS.gene51169:intron | 25.0% |
| !! | TTTAAGATAAGGAAAGTTAG+AGG | + | chr7.3:27467299-27467318 | None:intergenic | 25.0% |
| ATGAAATGGACATGGAAATA+GGG | - | chr7.3:27467592-27467611 | MS.gene51169:intron | 30.0% | |
| CTCGATCATCTCAATATATA+AGG | - | chr7.3:27467199-27467218 | MS.gene51169:intron | 30.0% | |
| GAAGAAATACGTGTCAATAA+TGG | - | chr7.3:27467239-27467258 | MS.gene51169:intron | 30.0% | |
| TATCAATCAACTTCATCCAA+CGG | + | chr7.3:27466484-27466503 | None:intergenic | 30.0% | |
| TCATCTCAATATATAAGGTG+TGG | - | chr7.3:27467204-27467223 | MS.gene51169:intron | 30.0% | |
| TCTCATACCTATACTTGTTT+GGG | + | chr7.3:27466641-27466660 | None:intergenic | 30.0% | |
| TTCTCATACCTATACTTGTT+TGG | + | chr7.3:27466642-27466661 | None:intergenic | 30.0% | |
| AAGAGACTTATTGCATCATG+TGG | + | chr7.3:27466137-27466156 | None:intergenic | 35.0% | |
| AGAAGAAGATGAAATGGACA+TGG | - | chr7.3:27467584-27467603 | MS.gene51169:intron | 35.0% | |
| AGATAAGGAAAGTTAGAGGT+AGG | + | chr7.3:27467295-27467314 | None:intergenic | 35.0% | |
| ATAAGGAAAGTTAGAGGTAG+GGG | + | chr7.3:27467293-27467312 | None:intergenic | 35.0% | |
| CACTCCGACTCTATAAAATT+GGG | + | chr7.3:27467322-27467341 | None:intergenic | 35.0% | |
| CTCATACCTATACTTGTTTG+GGG | + | chr7.3:27466640-27466659 | None:intergenic | 35.0% | |
| GATAAGGAAAGTTAGAGGTA+GGG | + | chr7.3:27467294-27467313 | None:intergenic | 35.0% | |
| GATGAAATGGACATGGAAAT+AGG | - | chr7.3:27467591-27467610 | MS.gene51169:intron | 35.0% | |
| GGACTTAGAAGAAGATGAAA+TGG | - | chr7.3:27467578-27467597 | MS.gene51169:intron | 35.0% | |
| TAGTCATTCGATAAAGGACT+TGG | - | chr7.3:27466332-27466351 | MS.gene51169:intron | 35.0% | |
| TATGACAACATACCACCATA+TGG | + | chr7.3:27466413-27466432 | None:intergenic | 35.0% | |
| TCACATGTTGTACATCTGTT+GGG | + | chr7.3:27467620-27467639 | None:intergenic | 35.0% | |
| TCATACCTATACTTGTTTGG+GGG | + | chr7.3:27466639-27466658 | None:intergenic | 35.0% | |
| TCCTTCAACAAGTTGTTCAA+AGG | - | chr7.3:27466948-27466967 | MS.gene51169:intron | 35.0% | |
| TTGAGATGATCGAGACTAAA+AGG | + | chr7.3:27467193-27467212 | None:intergenic | 35.0% | |
| ! | AATAGAAGGCATGATGTTGA+TGG | + | chr7.3:27467775-27467794 | None:intergenic | 35.0% |
| ! | ATAGAAGGCATGATGTTGAT+GGG | + | chr7.3:27467774-27467793 | None:intergenic | 35.0% |
| ! | ATGGGAGATGTTTCATGTTT+TGG | + | chr7.3:27467756-27467775 | None:intergenic | 35.0% |
| !! | AACTTGTTGAAGGATAGGTT+AGG | + | chr7.3:27466942-27466961 | None:intergenic | 35.0% |
| !! | TGAACAACTTGTTGAAGGAT+AGG | + | chr7.3:27466947-27466966 | None:intergenic | 35.0% |
| !! | TTTTGGCTGTAAAGATTGAG+AGG | + | chr7.3:27467739-27467758 | None:intergenic | 35.0% |
| AAAGTTAGAGGTAGGGGAAA+TGG | + | chr7.3:27467287-27467306 | None:intergenic | 40.0% | |
| AACATGTGACACACATTGGT+TGG | - | chr7.3:27467631-27467650 | MS.gene51169:intron | 40.0% | |
| AATTGCAGTGGAGAAAGAAG+AGG | - | chr7.3:27467557-27467576 | MS.gene51169:intron | 40.0% | |
| CCACTCCGACTCTATAAAAT+TGG | + | chr7.3:27467323-27467342 | None:intergenic | 40.0% | |
| GTACAACATGTGACACACAT+TGG | - | chr7.3:27467627-27467646 | MS.gene51169:intron | 40.0% | |
| GTCACATGTTGTACATCTGT+TGG | + | chr7.3:27467621-27467640 | None:intergenic | 40.0% | |
| TTCTTCCCCCAAACAAGTAT+AGG | - | chr7.3:27466631-27466650 | MS.gene51169:intron | 40.0% | |
| TTGAAGGATAGGTTAGGCTT+TGG | + | chr7.3:27466936-27466955 | None:intergenic | 40.0% | |
| ! | ACATGTGACACACATTGGTT+GGG | - | chr7.3:27467632-27467651 | MS.gene51169:intron | 40.0% |
| ! | CCAATTTTATAGAGTCGGAG+TGG | - | chr7.3:27467320-27467339 | MS.gene51169:intron | 40.0% |
| !! | AAGAAAGTGTTGCCATATGG+TGG | - | chr7.3:27466398-27466417 | MS.gene51169:intron | 40.0% |
| !! | CTGCAGGTTTTGATCTTCTT+GGG | + | chr7.3:27466103-27466122 | None:intergenic | 40.0% |
| !! | GCCTTTGAACAACTTGTTGA+AGG | + | chr7.3:27466952-27466971 | None:intergenic | 40.0% |
| !! | TCTGCAGGTTTTGATCTTCT+TGG | + | chr7.3:27466104-27466123 | None:intergenic | 40.0% |
| !! | TGCAAGAAAGTGTTGCCATA+TGG | - | chr7.3:27466395-27466414 | MS.gene51169:intron | 40.0% |
| !!! | AACGATGTATTTTGACCCGA+TGG | - | chr7.3:27466548-27466567 | MS.gene51169:intron | 40.0% |
| AGAAGGATCTAAAGGAGTCG+AGG | - | chr7.3:27466851-27466870 | MS.gene51169:CDS | 45.0% | |
| GAGATACAACCAGCTGAGAA+AGG | + | chr7.3:27466050-27466069 | None:intergenic | 45.0% | |
| GAGGATTCAGAGACTTTGTC+TGG | - | chr7.3:27466870-27466889 | MS.gene51169:CDS | 45.0% | |
| GTCATTCTTCCTTTCTCAGC+TGG | - | chr7.3:27466038-27466057 | MS.gene51169:CDS | 45.0% | |
| ! | TCAACTTCATCCAACGGACT+CGG | + | chr7.3:27466478-27466497 | None:intergenic | 45.0% |
| ! | TGTGGTGAATTAGTCTCTGC+AGG | + | chr7.3:27466119-27466138 | None:intergenic | 45.0% |
| !! | GGTTTTGATCTTCTTGGGTG+TGG | + | chr7.3:27466098-27466117 | None:intergenic | 45.0% |
| !! | AAAAGATATAAATTAATATA+TGG | + | chr7.3:27467043-27467062 | None:intergenic | 5.0% |
| AGAGAATCCCATCCCCTCAT+TGG | + | chr7.3:27467690-27467709 | None:intergenic | 50.0% | |
| AGGACTTGGATTCTCTCCCA+CGG | - | chr7.3:27466346-27466365 | MS.gene51169:intron | 50.0% | |
| AGTCGGAGTGGTGAGAAGAT+GGG | - | chr7.3:27467332-27467351 | MS.gene51169:intron | 50.0% | |
| CTCAGCTGGTTGTATCTCTG+AGG | - | chr7.3:27466052-27466071 | MS.gene51169:CDS | 50.0% | |
| GAGAATCCCATCCCCTCATT+GGG | + | chr7.3:27467689-27467708 | None:intergenic | 50.0% | |
| GATTCTCTCCCACGGTACAT+CGG | - | chr7.3:27466354-27466373 | MS.gene51169:intron | 50.0% | |
| TCATGACCCAATGAGGGGAT+GGG | - | chr7.3:27467680-27467699 | MS.gene51169:CDS | 50.0% | |
| TGCTGCTCATGACCCAATGA+GGG | - | chr7.3:27467674-27467693 | MS.gene51169:CDS | 50.0% | |
| TTGGTGAGTATGCAAGCGTG+TGG | - | chr7.3:27466994-27467013 | MS.gene51169:intron | 50.0% | |
| ! | GTGACACACATTGGTTGGGA+TGG | - | chr7.3:27467636-27467655 | MS.gene51169:intron | 50.0% |
| ! | TCTGAGGCTAGTGTTGCTGT+TGG | - | chr7.3:27466068-27466087 | MS.gene51169:CDS | 50.0% |
| ! | TTGTTGCACCGATGTACCGT+GGG | + | chr7.3:27466365-27466384 | None:intergenic | 50.0% |
| ! | TTTGTTGCACCGATGTACCG+TGG | + | chr7.3:27466366-27466385 | None:intergenic | 50.0% |
| CTCATGACCCAATGAGGGGA+TGG | - | chr7.3:27467679-27467698 | MS.gene51169:CDS | 55.0% | |
| GAGAGAAGATCCGAGTCCGT+TGG | - | chr7.3:27466465-27466484 | MS.gene51169:intron | 55.0% | |
| GAGGCTACAGAGAGAAGCTC+AGG | + | chr7.3:27467720-27467739 | None:intergenic | 55.0% | |
| GAGTCGGAGTGGTGAGAAGA+TGG | - | chr7.3:27467331-27467350 | MS.gene51169:intron | 55.0% | |
| GCTACAGAGAGAAGCTCAGG+AGG | + | chr7.3:27467717-27467736 | None:intergenic | 55.0% | |
| GCTGCTCATGACCCAATGAG+GGG | - | chr7.3:27467675-27467694 | MS.gene51169:CDS | 55.0% | |
| TGGGTCATGAGCAGCACATG+AGG | + | chr7.3:27467670-27467689 | None:intergenic | 55.0% | |
| ! | GTGCTGCTCATGACCCAATG+AGG | - | chr7.3:27467673-27467692 | MS.gene51169:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 27466014 | 27467800 | 27466014 | ID=MS.gene51169 |
| chr7.3 | mRNA | 27466014 | 27467800 | 27466014 | ID=MS.gene51169.t1;Parent=MS.gene51169 |
| chr7.3 | exon | 27467665 | 27467800 | 27467665 | ID=MS.gene51169.t1.exon1;Parent=MS.gene51169.t1 |
| chr7.3 | CDS | 27467665 | 27467800 | 27467665 | ID=cds.MS.gene51169.t1;Parent=MS.gene51169.t1 |
| chr7.3 | exon | 27466818 | 27466961 | 27466818 | ID=MS.gene51169.t1.exon2;Parent=MS.gene51169.t1 |
| chr7.3 | CDS | 27466818 | 27466961 | 27466818 | ID=cds.MS.gene51169.t1;Parent=MS.gene51169.t1 |
| chr7.3 | exon | 27466014 | 27466249 | 27466014 | ID=MS.gene51169.t1.exon3;Parent=MS.gene51169.t1 |
| chr7.3 | CDS | 27466014 | 27466249 | 27466014 | ID=cds.MS.gene51169.t1;Parent=MS.gene51169.t1 |
| Gene Sequence |
| Protein sequence |
