AlfalfaGEDB | MS.gene51423

M. sativa cultivar XinJiangDaYe / MS.gene51423

KEGG: -
GO: -
PFAM: PF14383.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51423.t1	XP_003594803.1	98.4	189	2	1	1	189	51	238	2.00E-96	362.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51423.t1	G7IMV9	98.4	189	2	1	1	189	51	238	1.4e-96	362.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055578	MS.gene51423	0.808919	2.44E-50	-1.69E-46
MS.gene056041	MS.gene51423	0.837968	3.80E-57	-1.69E-46
MS.gene058196	MS.gene51423	0.929042	1.22E-92	-1.69E-46
MS.gene058197	MS.gene51423	0.86208	6.53E-64	-1.69E-46
MS.gene058198	MS.gene51423	0.860271	2.32E-63	-1.69E-46
MS.gene058478	MS.gene51423	0.802864	4.58E-49	-1.69E-46
MS.gene061099	MS.gene51423	0.817455	3.27E-52	-1.69E-46
MS.gene061260	MS.gene51423	0.814841	1.25E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51423.t1	MTR_2g034810	94.954	218	10	1	1	218	51	267	5.72e-143	422
MS.gene51423.t1	MTR_7g112030	41.951	205	99	5	5	193	51	251	8.88e-37	137
MS.gene51423.t1	MTR_1g083850	36.559	186	101	5	10	191	2	174	7.32e-17	79.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51423.t1	AT5G26910	41.414	198	103	3	6	190	53	250	1.20e-35	133
MS.gene51423.t1	AT5G26910	41.414	198	103	3	6	190	52	249	2.72e-35	132
MS.gene51423.t1	AT5G26910	41.414	198	103	3	6	190	53	250	2.77e-35	132
MS.gene51423.t1	AT5G26910	41.414	198	103	3	6	190	53	250	2.77e-35	132
MS.gene51423.t1	AT3G05750	42.105	171	85	3	34	191	90	259	4.44e-33	126
MS.gene51423.t1	AT3G05750	42.105	171	85	3	34	191	82	251	5.20e-33	126
MS.gene51423.t1	AT3G58650	45.143	175	84	5	27	191	78	250	1.05e-31	122
MS.gene51423.t1	AT3G05750	40.127	157	80	3	48	191	1	156	4.94e-27	108
MS.gene51423.t1	AT3G05750	40.127	157	80	3	48	191	1	156	4.94e-27	108

Find 58 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAATTGATCTTGCCGATTT+TGG	0.126247	2.2:-54165931	None:intergenic
GATTCTCAGTTTCTATATTC+TGG	0.225407	2.2:+54165802	MS.gene51423:CDS
ACAAAACCGGGATTCTTAAT+AGG	0.234211	2.2:-54165979	None:intergenic
TAATAGGGGACAAAAGTTTA+TGG	0.239442	2.2:-54165963	None:intergenic
TAATGTAGTTGCAAGGCTTA+TGG	0.290220	2.2:+54165705	MS.gene51423:CDS
CAACAATCCTGCTTATATAA+TGG	0.326959	2.2:+54166008	MS.gene51423:CDS
AATGTAGTTGCAAGGCTTAT+GGG	0.343622	2.2:+54165706	MS.gene51423:CDS
CAAAACCGGGATTCTTAATA+GGG	0.350999	2.2:-54165978	None:intergenic
AAAAGTTTATGGTGTGTAAC+AGG	0.355362	2.2:-54165952	None:intergenic
TTTGCCTCCTTCCGGTTTCT+CGG	0.358423	2.2:+54165738	MS.gene51423:CDS
GGGATCTTCTAGCAATTTCA+TGG	0.358544	2.2:+54165846	MS.gene51423:CDS
TTAGATTCTTTGCCTCCTTC+CGG	0.359642	2.2:+54165730	MS.gene51423:CDS
ATAAGCCTTGCAACTACATT+AGG	0.385722	2.2:-54165703	None:intergenic
AGCAAAGGCTAAAGCGCATT+TGG	0.386167	2.2:+54166068	MS.gene51423:CDS
AATGCGCTTTAGCCTTTGCT+TGG	0.391802	2.2:-54166065	None:intergenic
GGCAATGAGACATCCCTATC+TGG	0.407656	2.2:+54165481	None:intergenic
GATTGATATTGCTACCAGAT+AGG	0.414071	2.2:-54165495	None:intergenic
ATAGAAACTGAGAATCTTGA+AGG	0.414969	2.2:-54165795	None:intergenic
AGAAGTATTGCCTCCAAAAT+CGG	0.425187	2.2:+54165918	MS.gene51423:CDS
CAGGTCGATGAAGACGAAAA+TGG	0.438366	2.2:+54165598	MS.gene51423:intron
ATTGTTGGATGGAACAAAAC+CGG	0.440286	2.2:-54165992	None:intergenic
ATGAAGACGAAAATGGTGTT+GGG	0.440544	2.2:+54165605	MS.gene51423:CDS
CCGCAAGAATTCTTGAACCT+GGG	0.445062	2.2:+54166037	MS.gene51423:CDS
ACTGAAGATGTCGCAATAAG+AGG	0.450581	2.2:-54166156	None:intergenic
GTTTCTATATTCTGGAAAGT+TGG	0.452052	2.2:+54165810	MS.gene51423:CDS
CGCTTTAGCCTTTGCTTGGG+AGG	0.453823	2.2:-54166061	None:intergenic
CATGGAGTCAAAGCCTCAAA+AGG	0.460709	2.2:+54165864	MS.gene51423:CDS
ATGCGCTTTAGCCTTTGCTT+GGG	0.471863	2.2:-54166064	None:intergenic
GCCGCAAGAATTCTTGAACC+TGG	0.480122	2.2:+54166036	MS.gene51423:CDS
CATTATATAAGCAGGATTGT+TGG	0.482949	2.2:-54166007	None:intergenic
CATCATCGACGGTCTCACTA+AGG	0.485114	2.2:+54166091	MS.gene51423:CDS
CGAGTAGGGATCCGAGAAAC+CGG	0.493083	2.2:-54165749	None:intergenic
TTGTCCCCTATTAAGAATCC+CGG	0.498971	2.2:+54165973	MS.gene51423:CDS
GGTTAAAGATCTTAGAGATA+AGG	0.502958	2.2:+54166113	MS.gene51423:CDS
ATTGATATTGCTACCAGATA+GGG	0.509200	2.2:-54165494	None:intergenic
GGTGTTGGGGAATATGTTAG+AGG	0.527966	2.2:+54165619	MS.gene51423:CDS
GCCTTTGCTTGGGAGGACCC+AGG	0.534090	2.2:-54166054	None:intergenic
GCAGCCTCCATTATATAAGC+AGG	0.537047	2.2:-54166015	None:intergenic
GATGAAGACGAAAATGGTGT+TGG	0.540397	2.2:+54165604	MS.gene51423:CDS
AGCGCATTTGGCATCATCGA+CGG	0.545183	2.2:+54166080	MS.gene51423:CDS
TTGTTGGATGGAACAAAACC+GGG	0.552886	2.2:-54165991	None:intergenic
TAAAGATCTTAGAGATAAGG+TGG	0.573035	2.2:+54166116	MS.gene51423:CDS
CCCAGGTTCAAGAATTCTTG+CGG	0.576295	2.2:-54166037	None:intergenic
AAGGTGGACTCTTCGCAGAA+AGG	0.576510	2.2:+54166132	MS.gene51423:CDS
ATATAAGCAGGATTGTTGGA+TGG	0.580763	2.2:-54166003	None:intergenic
ACCTGGGTCCTCCCAAGCAA+AGG	0.594584	2.2:+54166053	MS.gene51423:CDS
ATCATCGACGGTCTCACTAA+GGG	0.595032	2.2:+54166092	MS.gene51423:CDS
CAATCCTGCTTATATAATGG+AGG	0.595285	2.2:+54166011	MS.gene51423:CDS
AAATTTCTCAAGAAGAAGCA+AGG	0.612296	2.2:+54166214	MS.gene51423:CDS
TCTCATCAGAATCTACTCAA+AGG	0.615246	2.2:+54166237	MS.gene51423:CDS
TAGAAACTGAGAATCTTGAA+GGG	0.623762	2.2:-54165794	None:intergenic
ACACTTCACAGCATTCGACT+CGG	0.634714	2.2:-54166264	None:intergenic
TAGGGATCCGAGAAACCGGA+AGG	0.638892	2.2:-54165745	None:intergenic
GAGCTCCTAATGTAGTTGCA+AGG	0.641567	2.2:+54165698	MS.gene51423:CDS
TGAAGACGAAAATGGTGTTG+GGG	0.648206	2.2:+54165606	MS.gene51423:CDS
GGATCCGAGAAACCGGAAGG+AGG	0.677160	2.2:-54165742	None:intergenic
AAAACCGGGATTCTTAATAG+GGG	0.690424	2.2:-54165977	None:intergenic
GTTACAAGTGATGACACATG+TGG	0.725091	2.2:+54165670	MS.gene51423:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAATTATGTACAAGTAAC+AGG	-	chr2.2:54165539-54165558	None:intergenic	20.0%
!	ATTACATGTTTAATTCTTCC+AGG	+	chr2.2:54165579-54165598	MS.gene51423:intron	25.0%
!!!	TCTCTTTGAAACTTTTCAAT+TGG	-	chr2.2:54165901-54165920	None:intergenic	25.0%
	AAAAGTTTATGGTGTGTAAC+AGG	-	chr2.2:54165955-54165974	None:intergenic	30.0%
	AAATTTCTCAAGAAGAAGCA+AGG	+	chr2.2:54166214-54166233	MS.gene51423:CDS	30.0%
	ATAGAAACTGAGAATCTTGA+AGG	-	chr2.2:54165798-54165817	None:intergenic	30.0%
	ATTGATATTGCTACCAGATA+GGG	-	chr2.2:54165497-54165516	None:intergenic	30.0%
	CAACAATCCTGCTTATATAA+TGG	+	chr2.2:54166008-54166027	MS.gene51423:CDS	30.0%
	CATTATATAAGCAGGATTGT+TGG	-	chr2.2:54166010-54166029	None:intergenic	30.0%
	GATTCTCAGTTTCTATATTC+TGG	+	chr2.2:54165802-54165821	MS.gene51423:CDS	30.0%
	GGTTAAAGATCTTAGAGATA+AGG	+	chr2.2:54166113-54166132	MS.gene51423:CDS	30.0%
	TAAAGATCTTAGAGATAAGG+TGG	+	chr2.2:54166116-54166135	MS.gene51423:CDS	30.0%
	TAATAGGGGACAAAAGTTTA+TGG	-	chr2.2:54165966-54165985	None:intergenic	30.0%
	TAGAAACTGAGAATCTTGAA+GGG	-	chr2.2:54165797-54165816	None:intergenic	30.0%
!	AAAGTTGGTTGAAAAAGTTG+AGG	+	chr2.2:54165825-54165844	MS.gene51423:CDS	30.0%
!	AAGTTGGTTGAAAAAGTTGA+GGG	+	chr2.2:54165826-54165845	MS.gene51423:CDS	30.0%
!	GTTTCTATATTCTGGAAAGT+TGG	+	chr2.2:54165810-54165829	MS.gene51423:CDS	30.0%
	AAAACCGGGATTCTTAATAG+GGG	-	chr2.2:54165980-54165999	None:intergenic	35.0%
	AATGTAGTTGCAAGGCTTAT+GGG	+	chr2.2:54165706-54165725	MS.gene51423:CDS	35.0%
	ACAAAACCGGGATTCTTAAT+AGG	-	chr2.2:54165982-54166001	None:intergenic	35.0%
	AGGGATCGTGTATCAAAAAA+TGG	-	chr2.2:54165778-54165797	None:intergenic	35.0%
	ATAAGCCTTGCAACTACATT+AGG	-	chr2.2:54165706-54165725	None:intergenic	35.0%
	ATATAAGCAGGATTGTTGGA+TGG	-	chr2.2:54166006-54166025	None:intergenic	35.0%
	ATGAAGACGAAAATGGTGTT+GGG	+	chr2.2:54165605-54165624	MS.gene51423:CDS	35.0%
	ATTGTTGGATGGAACAAAAC+CGG	-	chr2.2:54165995-54166014	None:intergenic	35.0%
	CAAAACCGGGATTCTTAATA+GGG	-	chr2.2:54165981-54166000	None:intergenic	35.0%
	CAATCCTGCTTATATAATGG+AGG	+	chr2.2:54166011-54166030	MS.gene51423:CDS	35.0%
	GATTGATATTGCTACCAGAT+AGG	-	chr2.2:54165498-54165517	None:intergenic	35.0%
	TAATGTAGTTGCAAGGCTTA+TGG	+	chr2.2:54165705-54165724	MS.gene51423:CDS	35.0%
	TCTCATCAGAATCTACTCAA+AGG	+	chr2.2:54166237-54166256	MS.gene51423:CDS	35.0%
!	GACATCTTCAGTAGCTTTTA+GGG	+	chr2.2:54166167-54166186	MS.gene51423:CDS	35.0%
!	GGTCTAGTAATAACCTTTTG+AGG	-	chr2.2:54165880-54165899	None:intergenic	35.0%
!!	AGAAGTATTGCCTCCAAAAT+CGG	+	chr2.2:54165918-54165937	MS.gene51423:CDS	35.0%
	ACTGAAGATGTCGCAATAAG+AGG	-	chr2.2:54166159-54166178	None:intergenic	40.0%
	GATGAAGACGAAAATGGTGT+TGG	+	chr2.2:54165604-54165623	MS.gene51423:CDS	40.0%
	GGGATCTTCTAGCAATTTCA+TGG	+	chr2.2:54165846-54165865	MS.gene51423:CDS	40.0%
	GTTACAAGTGATGACACATG+TGG	+	chr2.2:54165670-54165689	MS.gene51423:CDS	40.0%
	TTAGATTCTTTGCCTCCTTC+CGG	+	chr2.2:54165730-54165749	MS.gene51423:CDS	40.0%
	TTGTCCCCTATTAAGAATCC+CGG	+	chr2.2:54165973-54165992	MS.gene51423:CDS	40.0%
	TTGTTGGATGGAACAAAACC+GGG	-	chr2.2:54165994-54166013	None:intergenic	40.0%
!	ATCAAAAAATGGCGTCGAGT+AGG	-	chr2.2:54165767-54165786	None:intergenic	40.0%
!	CGACATCTTCAGTAGCTTTT+AGG	+	chr2.2:54166166-54166185	MS.gene51423:CDS	40.0%
!	TCAAAAAATGGCGTCGAGTA+GGG	-	chr2.2:54165766-54165785	None:intergenic	40.0%
!	TGAAGACGAAAATGGTGTTG+GGG	+	chr2.2:54165606-54165625	MS.gene51423:CDS	40.0%
!!	TTCAGTAGCTTTTAGGGACT+CGG	+	chr2.2:54166173-54166192	MS.gene51423:CDS	40.0%
!!!	ATTGATCTTGCCGATTTTGG+AGG	-	chr2.2:54165931-54165950	None:intergenic	40.0%
!!!	GGAATTGATCTTGCCGATTT+TGG	-	chr2.2:54165934-54165953	None:intergenic	40.0%
	AGCAAAGGCTAAAGCGCATT+TGG	+	chr2.2:54166068-54166087	MS.gene51423:CDS	45.0%
	ATCATCGACGGTCTCACTAA+GGG	+	chr2.2:54166092-54166111	MS.gene51423:CDS	45.0%
	CAGGTCGATGAAGACGAAAA+TGG	+	chr2.2:54165598-54165617	MS.gene51423:intron	45.0%
	CATGGAGTCAAAGCCTCAAA+AGG	+	chr2.2:54165864-54165883	MS.gene51423:CDS	45.0%
	CCCAGGTTCAAGAATTCTTG+CGG	-	chr2.2:54166040-54166059	None:intergenic	45.0%
	CCGCAAGAATTCTTGAACCT+GGG	+	chr2.2:54166037-54166056	MS.gene51423:CDS	45.0%
	GAGCTCCTAATGTAGTTGCA+AGG	+	chr2.2:54165698-54165717	MS.gene51423:CDS	45.0%
	GCAGCCTCCATTATATAAGC+AGG	-	chr2.2:54166018-54166037	None:intergenic	45.0%
!	AATGCGCTTTAGCCTTTGCT+TGG	-	chr2.2:54166068-54166087	None:intergenic	45.0%
!	ATGCGCTTTAGCCTTTGCTT+GGG	-	chr2.2:54166067-54166086	None:intergenic	45.0%
!	CATTTTCGTCTTCATCGACC+TGG	-	chr2.2:54165600-54165619	None:intergenic	45.0%
!	GGTGTTGGGGAATATGTTAG+AGG	+	chr2.2:54165619-54165638	MS.gene51423:CDS	45.0%
!!	TCAGTAGCTTTTAGGGACTC+GGG	+	chr2.2:54166174-54166193	MS.gene51423:CDS	45.0%
	AGCGCATTTGGCATCATCGA+CGG	+	chr2.2:54166080-54166099	MS.gene51423:CDS	50.0%
	CATCATCGACGGTCTCACTA+AGG	+	chr2.2:54166091-54166110	MS.gene51423:CDS	50.0%
	GCCGCAAGAATTCTTGAACC+TGG	+	chr2.2:54166036-54166055	MS.gene51423:CDS	50.0%
	TTTGCCTCCTTCCGGTTTCT+CGG	+	chr2.2:54165738-54165757	MS.gene51423:CDS	50.0%
!	AAGGTGGACTCTTCGCAGAA+AGG	+	chr2.2:54166132-54166151	MS.gene51423:CDS	50.0%
	CGAGTAGGGATCCGAGAAAC+CGG	-	chr2.2:54165752-54165771	None:intergenic	55.0%
	TAGGGATCCGAGAAACCGGA+AGG	-	chr2.2:54165748-54165767	None:intergenic	55.0%
!	CGCTTTAGCCTTTGCTTGGG+AGG	-	chr2.2:54166064-54166083	None:intergenic	55.0%
	ACCTGGGTCCTCCCAAGCAA+AGG	+	chr2.2:54166053-54166072	MS.gene51423:CDS	60.0%
	GGATCCGAGAAACCGGAAGG+AGG	-	chr2.2:54165745-54165764	None:intergenic	60.0%
	GCCTTTGCTTGGGAGGACCC+AGG	-	chr2.2:54166057-54166076	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	54165485	54166281	54165485	ID=MS.gene51423
chr2.2	mRNA	54165485	54166281	54165485	ID=MS.gene51423.t1;Parent=MS.gene51423
chr2.2	exon	54165485	54165502	54165485	ID=MS.gene51423.t1.exon1;Parent=MS.gene51423.t1
chr2.2	CDS	54165485	54165502	54165485	ID=cds.MS.gene51423.t1;Parent=MS.gene51423.t1
chr2.2	exon	54165601	54166281	54165601	ID=MS.gene51423.t1.exon2;Parent=MS.gene51423.t1
chr2.2	CDS	54165601	54166281	54165601	ID=cds.MS.gene51423.t1;Parent=MS.gene51423.t1

Gene Sequence

>MS.gene51423

ATGAGACATCCCTATCTGGTAGCAATATCAATCACTATATCTTCAATTTTACCTGTTACTTGTACATAATTTTTTGTTATGAAATTAGCTAATTATTACATGTTTAATTCTTCCAGGTCGATGAAGACGAAAATGGTGTTGGGGAATATGTTAGAGGAAGCTGTGATCATAGTTATGCTTCATCTGTTACAAGTGATGACACATGTGGAACAAGAGCTCCTAATGTAGTTGCAAGGCTTATGGGATTAGATTCTTTGCCTCCTTCCGGTTTCTCGGATCCCTACTCGACGCCATTTTTTGATACACGATCCCTTCAAGATTCTCAGTTTCTATATTCTGGAAAGTTGGTTGAAAAAGTTGAGGGATCTTCTAGCAATTTCATGGAGTCAAAGCCTCAAAAGGTTATTACTAGACCAATTGAAAAGTTTCAAAGAGAAGTATTGCCTCCAAAATCGGCAAGATCAATTCCTGTTACACACCATAAACTTTTGTCCCCTATTAAGAATCCCGGTTTTGTTCCATCCAACAATCCTGCTTATATAATGGAGGCTGCCGCAAGAATTCTTGAACCTGGGTCCTCCCAAGCAAAGGCTAAAGCGCATTTGGCATCATCGACGGTCTCACTAAGGGTTAAAGATCTTAGAGATAAGGTGGACTCTTCGCAGAAAGGACCTCTTATTGCGACATCTTCAGTAGCTTTTAGGGACTCGGGAGCTAAAAGAAAAAGAGAAATTTCTCAAGAAGAAGCAAGGTCTCATCAGAATCTACTCAAAGGTCAGCCGAGTCGAATGCTGTGA

Protein sequence

>MS.gene51423.t1

MRHPYLVDEDENGVGEYVRGSCDHSYASSVTSDDTCGTRAPNVVARLMGLDSLPPSGFSDPYSTPFFDTRSLQDSQFLYSGKLVEKVEGSSSNFMESKPQKVITRPIEKFQREVLPPKSARSIPVTHHKLLSPIKNPGFVPSNNPAYIMEAAARILEPGSSQAKAKAHLASSTVSLRVKDLRDKVDSSQKGPLIATSSVAFRDSGAKRKREISQEEARSHQNLLKGQPSRML