Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51618.t1 | XP_003598248.1 | 98.2 | 489 | 9 | 0 | 1 | 489 | 1 | 489 | 1.50E-271 | 944.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51618.t1 | Q12725 | 32.0 | 337 | 182 | 11 | 193 | 485 | 71 | 404 | 1.7e-37 | 158.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51618.t1 | G7IYK2 | 98.2 | 489 | 9 | 0 | 1 | 489 | 1 | 489 | 1.1e-271 | 944.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054146 | MS.gene51618 | 0.803298 | 3.73E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51618.t1 | MTR_3g008960 | 98.160 | 489 | 9 | 0 | 1 | 489 | 1 | 489 | 0.0 | 1004 |
| MS.gene51618.t1 | MTR_3g009010 | 89.206 | 491 | 51 | 1 | 1 | 489 | 72 | 562 | 0.0 | 904 |
| MS.gene51618.t1 | MTR_3g009030 | 79.719 | 498 | 91 | 5 | 1 | 489 | 1 | 497 | 0.0 | 785 |
| MS.gene51618.t1 | MTR_3g009020 | 79.487 | 468 | 92 | 2 | 26 | 489 | 6 | 473 | 0.0 | 773 |
| MS.gene51618.t1 | MTR_8g091310 | 60.558 | 502 | 179 | 7 | 6 | 489 | 6 | 506 | 0.0 | 627 |
| MS.gene51618.t1 | MTR_5g062190 | 53.280 | 503 | 220 | 8 | 1 | 489 | 6 | 507 | 0.0 | 547 |
| MS.gene51618.t1 | MTR_5g062290 | 53.347 | 493 | 218 | 8 | 8 | 489 | 14 | 505 | 0.0 | 536 |
| MS.gene51618.t1 | MTR_3g086750 | 46.600 | 500 | 245 | 11 | 9 | 489 | 9 | 505 | 6.81e-153 | 446 |
| MS.gene51618.t1 | MTR_5g061980 | 37.634 | 279 | 109 | 9 | 158 | 430 | 87 | 306 | 1.80e-47 | 167 |
| MS.gene51618.t1 | MTR_4g035760 | 47.967 | 123 | 63 | 1 | 368 | 489 | 57 | 179 | 7.12e-38 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 107 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACATGATCACATAAGTATT+TGG | 0.123305 | 3.4:+4066141 | None:intergenic |
| CTATTATTTGAGCTTCTTAT+TGG | 0.155909 | 3.4:-4072515 | MS.gene51618:CDS |
| GCTTACAAAGCTGTGAATTT+AGG | 0.195268 | 3.4:-4072457 | MS.gene51618:CDS |
| AAGCATCAAATGGTCTATTT+TGG | 0.202544 | 3.4:-4071091 | MS.gene51618:CDS |
| CAGATTATCAAGGCACAAAT+TGG | 0.214773 | 3.4:-4070381 | MS.gene51618:CDS |
| CTTCTGGTTGGGAAACATTC+AGG | 0.224679 | 3.4:-4071387 | MS.gene51618:CDS |
| TTCAGATTTATGTCACAAAA+TGG | 0.249547 | 3.4:-4070133 | MS.gene51618:CDS |
| CAACATTTGGATGGGCCTAT+TGG | 0.262117 | 3.4:-4065719 | MS.gene51618:CDS |
| ATGAAACATTTCAGATTATA+AGG | 0.262921 | 3.4:-4071145 | MS.gene51618:CDS |
| GCCAACCGTGGTTCTGCTTC+TGG | 0.263236 | 3.4:-4071403 | MS.gene51618:CDS |
| GTTTAGGTGGGTTTGGATCT+TGG | 0.275341 | 3.4:+4070071 | None:intergenic |
| GCAGTTGAAGATTCTCAAAA+TGG | 0.275592 | 3.4:-4066728 | MS.gene51618:CDS |
| TTTAGGTGGGTTTGGATCTT+GGG | 0.277322 | 3.4:+4070072 | None:intergenic |
| TCATAGAATTCTTAGCAAAA+AGG | 0.286458 | 3.4:-4066634 | MS.gene51618:intron |
| ATGGTTATATTGACATTTAT+AGG | 0.303157 | 3.4:+4065695 | None:intergenic |
| ATAGCCTGAAGAACTATTAC+AGG | 0.305528 | 3.4:-4066194 | MS.gene51618:CDS |
| CTTCCCTGTAATAGTTCTTC+AGG | 0.319286 | 3.4:+4066190 | None:intergenic |
| TTATAATCTGAAATGTTTCA+TGG | 0.319759 | 3.4:+4071147 | None:intergenic |
| ACATGATCACATAAGTATTT+GGG | 0.338303 | 3.4:+4066142 | None:intergenic |
| ACAAAAGGTTTAGGTGGGTT+TGG | 0.353332 | 3.4:+4070064 | None:intergenic |
| AAGCTGTGAATTTAGGAAAC+TGG | 0.354101 | 3.4:-4072450 | MS.gene51618:CDS |
| AATTTGAATTTGCTACAATT+TGG | 0.358353 | 3.4:+4071180 | None:intergenic |
| AAGAAGCTCAAATAATAGTT+TGG | 0.360133 | 3.4:+4072520 | None:intergenic |
| CCGTGGTTCTGCTTCTGGTT+GGG | 0.360673 | 3.4:-4071398 | MS.gene51618:CDS |
| AACATTTGGATGGGCCTATT+GGG | 0.362817 | 3.4:-4065718 | MS.gene51618:CDS |
| TTGACATTTATAGGCCCAAT+AGG | 0.385790 | 3.4:+4065704 | None:intergenic |
| GTTAAACACTCTAAAATTGA+AGG | 0.404716 | 3.4:+4071232 | None:intergenic |
| GTGTATTCTCATGCAACATT+TGG | 0.419146 | 3.4:-4065732 | MS.gene51618:CDS |
| TGGCTCATTTGCAGCTTTGG+AGG | 0.420703 | 3.4:-4071268 | MS.gene51618:intron |
| TTTGTGCTTGGACATATCTC+TGG | 0.424881 | 3.4:+4065760 | None:intergenic |
| TACCAACTTACTAATGGTTA+TGG | 0.434769 | 3.4:-4070301 | MS.gene51618:CDS |
| ATGGAACCTTCTCGCTTTGA+CGG | 0.442568 | 3.4:-4072409 | MS.gene51618:CDS |
| ACATATCTTTCCGCTGAAGA+TGG | 0.444863 | 3.4:-4071445 | MS.gene51618:CDS |
| GCATTACTACAATATGTATT+CGG | 0.453344 | 3.4:-4066046 | MS.gene51618:CDS |
| ACCGTGGTTCTGCTTCTGGT+TGG | 0.462630 | 3.4:-4071399 | MS.gene51618:CDS |
| AACAAGAAATTTGTGGGGCA+AGG | 0.463143 | 3.4:-4071212 | MS.gene51618:CDS |
| GGCTCATTTGCAGCTTTGGA+GGG | 0.466108 | 3.4:-4071267 | MS.gene51618:intron |
| ACATAAGAAATGACAGAGCC+TGG | 0.466178 | 3.4:-4065984 | MS.gene51618:CDS |
| TTCTGGTTGGGAAACATTCA+GGG | 0.470013 | 3.4:-4071386 | MS.gene51618:intron |
| CATATCTTTCCGCTGAAGAT+GGG | 0.471301 | 3.4:-4071444 | MS.gene51618:CDS |
| TGTTTGCTTTGTAGGACCAT+TGG | 0.478787 | 3.4:-4070180 | MS.gene51618:intron |
| TGGGCTATCCACCATCCAAC+AGG | 0.479749 | 3.4:+4070091 | None:intergenic |
| GTGTTTAACAAGAAATTTGT+GGG | 0.481041 | 3.4:-4071218 | MS.gene51618:CDS |
| CTCAAGTGGTTGATCGAGAA+TGG | 0.484613 | 3.4:-4065666 | MS.gene51618:CDS |
| CTTCTTCTCTCATTTGTCAA+AGG | 0.486302 | 3.4:-4066093 | MS.gene51618:CDS |
| ATTCGAATCAAAGCATCAAA+TGG | 0.491455 | 3.4:-4071101 | MS.gene51618:CDS |
| TGCCATAACCATTAGTAAGT+TGG | 0.494045 | 3.4:+4070299 | None:intergenic |
| AATTGTCAACAAAGATCTCT+TGG | 0.496005 | 3.4:-4072386 | MS.gene51618:intron |
| TTTATAACTTGCAGTAAGCA+TGG | 0.498027 | 3.4:-4066776 | MS.gene51618:intron |
| ATAAATGTCAATATAACCAT+TGG | 0.502897 | 3.4:-4065692 | MS.gene51618:CDS |
| AAGAGATGGATATACAGAAT+GGG | 0.504441 | 3.4:-4066687 | MS.gene51618:CDS |
| ATGTATTCGGATAAATTCAA+TGG | 0.504587 | 3.4:-4066033 | MS.gene51618:CDS |
| CAAGAGATGGATATACAGAA+TGG | 0.505790 | 3.4:-4066688 | MS.gene51618:CDS |
| CTTCAGCGGAAAGATATGTC+TGG | 0.507610 | 3.4:+4071449 | None:intergenic |
| CACTTGACAATGCTTTCACA+TGG | 0.508127 | 3.4:-4070024 | MS.gene51618:CDS |
| GAGATATGTCCAAGCACAAA+TGG | 0.509273 | 3.4:-4065757 | MS.gene51618:CDS |
| GACAGAGCCTGGGATCTTAG+TGG | 0.512889 | 3.4:-4065973 | MS.gene51618:CDS |
| GGAGAGTACCAACTTACTAA+TGG | 0.515314 | 3.4:-4070307 | MS.gene51618:CDS |
| TTCTCATGCAACATTTGGAT+GGG | 0.516811 | 3.4:-4065727 | MS.gene51618:CDS |
| ATTCTCATGCAACATTTGGA+TGG | 0.521384 | 3.4:-4065728 | MS.gene51618:CDS |
| ACTGGCTTGTTGCTGAAGGA+TGG | 0.521485 | 3.4:-4072432 | MS.gene51618:CDS |
| ACAATTGTAAACACATTACA+AGG | 0.521868 | 3.4:-4070328 | MS.gene51618:CDS |
| AGTGTTTAACAAGAAATTTG+TGG | 0.525940 | 3.4:-4071219 | MS.gene51618:CDS |
| TTTCTTACTCGCATAACTTG+AGG | 0.526026 | 3.4:+4070268 | None:intergenic |
| AGATTATCAAGGCACAAATT+GGG | 0.526125 | 3.4:-4070380 | MS.gene51618:CDS |
| TGTGATCATGTCAAATCCAT+TGG | 0.526301 | 3.4:-4066130 | MS.gene51618:CDS |
| GAGCATAGTGGGACAAGAGA+TGG | 0.527467 | 3.4:-4066701 | MS.gene51618:CDS |
| AAATTTGTGGGGCAAGGAGA+AGG | 0.537779 | 3.4:-4071206 | MS.gene51618:CDS |
| AATACACATCCATTTGTGCT+TGG | 0.540193 | 3.4:+4065748 | None:intergenic |
| TAGGGGAATGGACAGCTGAA+TGG | 0.542964 | 3.4:-4065812 | MS.gene51618:CDS |
| GCATGGGATGAAAGTGATTG+TGG | 0.543506 | 3.4:-4066759 | MS.gene51618:CDS |
| ACAGCTGAATGGAGCATACA+AGG | 0.544710 | 3.4:-4065801 | MS.gene51618:CDS |
| CCCCAACAAAAGGTTTAGGT+GGG | 0.545640 | 3.4:+4070059 | None:intergenic |
| GCTTGTTGCTGAAGGATGGA+TGG | 0.547369 | 3.4:-4072428 | MS.gene51618:CDS |
| CAGTTATGTATGAATTCCAA+TGG | 0.561323 | 3.4:+4070164 | None:intergenic |
| GAGAAGAAGTTTAGAATCTG+TGG | 0.563821 | 3.4:+4066107 | None:intergenic |
| TTATAACTTGCAGTAAGCAT+GGG | 0.565682 | 3.4:-4066775 | MS.gene51618:intron |
| ATATCTTTCCGCTGAAGATG+GGG | 0.565840 | 3.4:-4071443 | MS.gene51618:CDS |
| AGTTTAGAATCTGTGGCCAA+TGG | 0.566970 | 3.4:+4066114 | None:intergenic |
| GGAAACTGGCTTGTTGCTGA+AGG | 0.571457 | 3.4:-4072436 | MS.gene51618:CDS |
| TGAAATGTTTCATGGTTGCT+AGG | 0.572223 | 3.4:+4071155 | None:intergenic |
| CTGTGAGGATACCTGTTGGA+TGG | 0.577394 | 3.4:-4070102 | MS.gene51618:CDS |
| TTGGGATGACAATGATCCAT+CGG | 0.585148 | 3.4:-4070362 | MS.gene51618:CDS |
| ACCAGAAGCAGAACCACGGT+TGG | 0.601567 | 3.4:+4071402 | None:intergenic |
| ACAATTCCGTCAAAGCGAGA+AGG | 0.606902 | 3.4:+4072403 | None:intergenic |
| TCAGTGACTGCAGATTATCA+AGG | 0.606979 | 3.4:-4070391 | MS.gene51618:CDS |
| TATCTCTGGTAGTCTTGCAT+AGG | 0.607680 | 3.4:+4065774 | None:intergenic |
| TATTGTAGTAATGCACATCA+AGG | 0.609097 | 3.4:+4066055 | None:intergenic |
| CATAAGAAATGACAGAGCCT+GGG | 0.612530 | 3.4:-4065983 | MS.gene51618:CDS |
| TGAGGATACCTGTTGGATGG+TGG | 0.627765 | 3.4:-4070099 | MS.gene51618:CDS |
| AGAGACACCACTAAGATCCC+AGG | 0.628976 | 3.4:+4065966 | None:intergenic |
| CTATCTAGATTGACACCTAG+TGG | 0.637297 | 3.4:+4066213 | None:intergenic |
| ATAACCATTGGAGTCTCAAG+TGG | 0.641339 | 3.4:-4065680 | MS.gene51618:CDS |
| CCCAACCAGAAGCAGAACCA+CGG | 0.648810 | 3.4:+4071398 | None:intergenic |
| TAAGCAAACGAAAGAGAGTG+AGG | 0.651768 | 3.4:+4072475 | None:intergenic |
| AGAGATGGATATACAGAATG+GGG | 0.651789 | 3.4:-4066686 | MS.gene51618:CDS |
| GGGAAGCTTATGATGTTGTG+AGG | 0.655321 | 3.4:-4066173 | MS.gene51618:CDS |
| TAGCCTGAAGAACTATTACA+GGG | 0.658921 | 3.4:-4066193 | MS.gene51618:CDS |
| TATCTTTCCGCTGAAGATGG+GGG | 0.660568 | 3.4:-4071442 | MS.gene51618:CDS |
| TCAACCACTTGAGACTCCAA+TGG | 0.670774 | 3.4:+4065676 | None:intergenic |
| TGTTTAACAAGAAATTTGTG+GGG | 0.672700 | 3.4:-4071217 | MS.gene51618:CDS |
| GATGCTGTGAGGATACCTGT+TGG | 0.677897 | 3.4:-4070106 | MS.gene51618:CDS |
| GAGCTGATGAATGAGCCACT+AGG | 0.678883 | 3.4:-4066228 | MS.gene51618:CDS |
| ACTTGACAATGCTTTCACAT+GGG | 0.683824 | 3.4:-4070023 | MS.gene51618:CDS |
| TCTATGACAGAGACTGTCTG+AGG | 0.686661 | 3.4:+4066650 | None:intergenic |
| ACTACTATTGTAGCCAACCG+TGG | 0.699222 | 3.4:-4071415 | MS.gene51618:CDS |
| AAAATGGATTAGATGCTGTG+AGG | 0.731300 | 3.4:-4070117 | MS.gene51618:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 4065650 | 4072551 | 4065650 | ID=MS.gene51618 |
| chr3.4 | mRNA | 4065650 | 4072551 | 4065650 | ID=MS.gene51618.t1;Parent=MS.gene51618 |
| chr3.4 | exon | 4072387 | 4072551 | 4072387 | ID=MS.gene51618.t1.exon1;Parent=MS.gene51618.t1 |
| chr3.4 | CDS | 4072387 | 4072551 | 4072387 | ID=cds.MS.gene51618.t1;Parent=MS.gene51618.t1 |
| chr3.4 | exon | 4071387 | 4071506 | 4071387 | ID=MS.gene51618.t1.exon2;Parent=MS.gene51618.t1 |
| chr3.4 | CDS | 4071387 | 4071506 | 4071387 | ID=cds.MS.gene51618.t1;Parent=MS.gene51618.t1 |
| chr3.4 | exon | 4071088 | 4071276 | 4071088 | ID=MS.gene51618.t1.exon3;Parent=MS.gene51618.t1 |
| chr3.4 | CDS | 4071088 | 4071276 | 4071088 | ID=cds.MS.gene51618.t1;Parent=MS.gene51618.t1 |
| chr3.4 | exon | 4070276 | 4070428 | 4070276 | ID=MS.gene51618.t1.exon4;Parent=MS.gene51618.t1 |
| chr3.4 | CDS | 4070276 | 4070428 | 4070276 | ID=cds.MS.gene51618.t1;Parent=MS.gene51618.t1 |
| chr3.4 | exon | 4070019 | 4070188 | 4070019 | ID=MS.gene51618.t1.exon5;Parent=MS.gene51618.t1 |
| chr3.4 | CDS | 4070019 | 4070188 | 4070019 | ID=cds.MS.gene51618.t1;Parent=MS.gene51618.t1 |
| chr3.4 | exon | 4066635 | 4066784 | 4066635 | ID=MS.gene51618.t1.exon6;Parent=MS.gene51618.t1 |
| chr3.4 | CDS | 4066635 | 4066784 | 4066635 | ID=cds.MS.gene51618.t1;Parent=MS.gene51618.t1 |
| chr3.4 | exon | 4065941 | 4066281 | 4065941 | ID=MS.gene51618.t1.exon7;Parent=MS.gene51618.t1 |
| chr3.4 | CDS | 4065941 | 4066281 | 4065941 | ID=cds.MS.gene51618.t1;Parent=MS.gene51618.t1 |
| chr3.4 | exon | 4065650 | 4065831 | 4065650 | ID=MS.gene51618.t1.exon8;Parent=MS.gene51618.t1 |
| chr3.4 | CDS | 4065650 | 4065831 | 4065650 | ID=cds.MS.gene51618.t1;Parent=MS.gene51618.t1 |
| Gene Sequence |
| Protein sequence |
