Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51709.t1 | XP_013467304.1 | 99 | 96 | 1 | 0 | 1 | 96 | 1 | 96 | 3.00E-43 | 184.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51709.t1 | I3S1M4 | 99.0 | 96 | 1 | 0 | 1 | 96 | 1 | 96 | 2.2e-43 | 184.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051757 | MS.gene51709 | 0.812145 | 4.91E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51709.t1 | MTR_1g048960 | 98.958 | 96 | 1 | 0 | 1 | 96 | 1 | 96 | 3.32e-66 | 194 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51709.t1 | AT1G72020 | 69.072 | 97 | 29 | 1 | 1 | 96 | 1 | 97 | 2.35e-45 | 141 |
Find 20 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCCTCTACTCACTGATT+TGG | 0.327769 | 3.4:-6788761 | None:intergenic |
| CTTGGACAAGATGAAGTATC+AGG | 0.339393 | 3.4:+6788864 | MS.gene51709:CDS |
| AGTAGAGGATTTCATTCAAC+TGG | 0.411924 | 3.4:+6788772 | MS.gene51709:CDS |
| CAGCAAAAGAAGACAACATC+TGG | 0.443589 | 3.4:+6788961 | MS.gene51709:CDS |
| AAGAGGATGGGAGGCGGACA+TGG | 0.484956 | 3.4:+6788799 | MS.gene51709:CDS |
| GTGTTCACAGCTTTCAGCAT+TGG | 0.501579 | 3.4:+6788907 | MS.gene51709:CDS |
| TGCATATCACATGTACAACT+TGG | 0.529028 | 3.4:+6788846 | MS.gene51709:CDS |
| TTCAACTGGTGTGAAGAGGA+TGG | 0.534302 | 3.4:+6788786 | MS.gene51709:CDS |
| TTCATTCAACTGGTGTGAAG+AGG | 0.561102 | 3.4:+6788782 | MS.gene51709:CDS |
| GCATATACTCTGGCTCGCTA+TGG | 0.570951 | 3.4:-6788822 | None:intergenic |
| ACTCACTGATTTGGAGATGG+AGG | 0.581472 | 3.4:-6788752 | None:intergenic |
| TCAACTGGTGTGAAGAGGAT+GGG | 0.584183 | 3.4:+6788787 | MS.gene51709:CDS |
| TGGAAAATGACTGCATACAC+AGG | 0.585917 | 3.4:-6788940 | None:intergenic |
| TCTACTCACTGATTTGGAGA+TGG | 0.595575 | 3.4:-6788755 | None:intergenic |
| GGTGTGAAGAGGATGGGAGG+CGG | 0.610714 | 3.4:+6788793 | MS.gene51709:CDS |
| TTGGACAAGATGAAGTATCA+GGG | 0.612137 | 3.4:+6788865 | MS.gene51709:CDS |
| ATCTCCAAATCAGTGAGTAG+AGG | 0.617769 | 3.4:+6788757 | MS.gene51709:CDS |
| TGATATGCATGCATATACTC+TGG | 0.624731 | 3.4:-6788832 | None:intergenic |
| ATAACGATGGCATTGAACAA+CGG | 0.641834 | 3.4:+6788691 | None:intergenic |
| ACTGGTGTGAAGAGGATGGG+AGG | 0.670600 | 3.4:+6788790 | MS.gene51709:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGTAGAGGATTTCATTCAAC+TGG | + | chr3.4:6788772-6788791 | MS.gene51709:CDS | 35.0% | |
| TGATATGCATGCATATACTC+TGG | - | chr3.4:6788835-6788854 | None:intergenic | 35.0% | |
| TGCATATCACATGTACAACT+TGG | + | chr3.4:6788846-6788865 | MS.gene51709:CDS | 35.0% | |
| ! | AAATCCTCTACTCACTGATT+TGG | - | chr3.4:6788764-6788783 | None:intergenic | 35.0% |
| ! | TTGGACAAGATGAAGTATCA+GGG | + | chr3.4:6788865-6788884 | MS.gene51709:CDS | 35.0% |
| ATCTCCAAATCAGTGAGTAG+AGG | + | chr3.4:6788757-6788776 | MS.gene51709:CDS | 40.0% | |
| CAGCAAAAGAAGACAACATC+TGG | + | chr3.4:6788961-6788980 | MS.gene51709:CDS | 40.0% | |
| TGGAAAATGACTGCATACAC+AGG | - | chr3.4:6788943-6788962 | None:intergenic | 40.0% | |
| TTCATTCAACTGGTGTGAAG+AGG | + | chr3.4:6788782-6788801 | MS.gene51709:CDS | 40.0% | |
| ! | CAGATGTTGTCTTCTTTTGC+TGG | - | chr3.4:6788963-6788982 | None:intergenic | 40.0% |
| ! | CTTGGACAAGATGAAGTATC+AGG | + | chr3.4:6788864-6788883 | MS.gene51709:CDS | 40.0% |
| !! | TCTACTCACTGATTTGGAGA+TGG | - | chr3.4:6788758-6788777 | None:intergenic | 40.0% |
| GTGTTCACAGCTTTCAGCAT+TGG | + | chr3.4:6788907-6788926 | MS.gene51709:CDS | 45.0% | |
| TCAACTGGTGTGAAGAGGAT+GGG | + | chr3.4:6788787-6788806 | MS.gene51709:CDS | 45.0% | |
| TTCAACTGGTGTGAAGAGGA+TGG | + | chr3.4:6788786-6788805 | MS.gene51709:CDS | 45.0% | |
| !! | ACTCACTGATTTGGAGATGG+AGG | - | chr3.4:6788755-6788774 | None:intergenic | 45.0% |
| ! | GCATATACTCTGGCTCGCTA+TGG | - | chr3.4:6788825-6788844 | None:intergenic | 50.0% |
| ACTGGTGTGAAGAGGATGGG+AGG | + | chr3.4:6788790-6788809 | MS.gene51709:CDS | 55.0% | |
| AAGAGGATGGGAGGCGGACA+TGG | + | chr3.4:6788799-6788818 | MS.gene51709:CDS | 60.0% | |
| GGTGTGAAGAGGATGGGAGG+CGG | + | chr3.4:6788793-6788812 | MS.gene51709:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 6788697 | 6788987 | 6788697 | ID=MS.gene51709 |
| chr3.4 | mRNA | 6788697 | 6788987 | 6788697 | ID=MS.gene51709.t1;Parent=MS.gene51709 |
| chr3.4 | exon | 6788697 | 6788987 | 6788697 | ID=MS.gene51709.t1.exon1;Parent=MS.gene51709.t1 |
| chr3.4 | CDS | 6788697 | 6788987 | 6788697 | ID=cds.MS.gene51709.t1;Parent=MS.gene51709.t1 |
| Gene Sequence |
| Protein sequence |
