AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051757


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051757.t1 MTR_7g104570 100.000 56 0 0 1 56 1 56 3.46e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051757.t1 AT3G52730 76.786 56 13 0 1 56 1 56 6.33e-27 92.8
MS.gene051757.t1 AT3G52730 76.786 56 13 0 1 56 1 56 6.81e-27 92.4

Find 15 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGAGGAGGAGGCGTTT+TGG 0.335531 7.4:+7684828 MS.gene051757:CDS
TCTGGGAAATAAATAATGTT+GGG 0.387527 7.4:+7685038 MS.gene051757:CDS
TCGTTGCAGGCTGTGGATTA+CGG 0.406296 7.4:+7685004 MS.gene051757:intron
ATTACGGTGTTCACAAGCTC+TGG 0.424543 7.4:+7685020 MS.gene051757:CDS
CTCTGGGAAATAAATAATGT+TGG 0.443669 7.4:+7685037 MS.gene051757:CDS
TATGTTACCTTCGTCATCGC+CGG 0.481783 7.4:+7684889 MS.gene051757:CDS
TTACGGTGTTCACAAGCTCT+GGG 0.588806 7.4:+7685021 MS.gene051757:CDS
CTGGGAAATAAATAATGTTG+GGG 0.603916 7.4:+7685039 MS.gene051757:CDS
GGCGCATAAGAACCTTGTAA+AGG 0.605705 7.4:-7684855 None:intergenic
GCTGCTAAAAGAAGAGGAGG+AGG 0.621931 7.4:+7684820 MS.gene051757:CDS
GAATCCGCTGCTAAAAGAAG+AGG 0.626583 7.4:+7684814 MS.gene051757:CDS
AAAAGCACCGGCGATGACGA+AGG 0.627325 7.4:-7684896 None:intergenic
TCCGCTGCTAAAAGAAGAGG+AGG 0.631604 7.4:+7684817 MS.gene051757:CDS
CCGTTCACCGAGAAAAGCAC+CGG 0.644147 7.4:-7684908 None:intergenic
AGGTAACATAGACAGAGTTG+CGG 0.713031 7.4:-7684876 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACGATTAGAGTAAAATTAAA+AGG - chr7.4:7684950-7684969 None:intergenic 20.0%
!! ATTAGAGTAAAATTAAAAGG+GGG - chr7.4:7684947-7684966 None:intergenic 20.0%
!! CGATTAGAGTAAAATTAAAA+GGG - chr7.4:7684949-7684968 None:intergenic 20.0%
!! GATTAGAGTAAAATTAAAAG+GGG - chr7.4:7684948-7684967 None:intergenic 20.0%
! TCTGGGAAATAAATAATGTT+GGG + chr7.4:7685038-7685057 MS.gene051757:CDS 25.0%
! CTCTGGGAAATAAATAATGT+TGG + chr7.4:7685037-7685056 MS.gene051757:CDS 30.0%
AGGTAACATAGACAGAGTTG+CGG - chr7.4:7684879-7684898 None:intergenic 40.0%
AGATCGCTGAATTTCGTTGC+AGG + chr7.4:7684991-7685010 MS.gene051757:intron 45.0%
ATTACGGTGTTCACAAGCTC+TGG + chr7.4:7685020-7685039 MS.gene051757:CDS 45.0%
GAATCCGCTGCTAAAAGAAG+AGG + chr7.4:7684814-7684833 MS.gene051757:CDS 45.0%
GGCGCATAAGAACCTTGTAA+AGG - chr7.4:7684858-7684877 None:intergenic 45.0%
TATGTTACCTTCGTCATCGC+CGG + chr7.4:7684889-7684908 MS.gene051757:CDS 45.0%
TTACGGTGTTCACAAGCTCT+GGG + chr7.4:7685021-7685040 MS.gene051757:CDS 45.0%
! TCCTCCTCTTCTTTTAGCAG+CGG - chr7.4:7684821-7684840 None:intergenic 45.0%
!!! CGTTTTGGAAGGCCTTTACA+AGG + chr7.4:7684843-7684862 MS.gene051757:CDS 45.0%
CTGAATTTCGTTGCAGGCTG+TGG + chr7.4:7684997-7685016 MS.gene051757:intron 50.0%
GCTGCTAAAAGAAGAGGAGG+AGG + chr7.4:7684820-7684839 MS.gene051757:CDS 50.0%
TCCGCTGCTAAAAGAAGAGG+AGG + chr7.4:7684817-7684836 MS.gene051757:CDS 50.0%
! TCGTTGCAGGCTGTGGATTA+CGG + chr7.4:7685004-7685023 MS.gene051757:intron 50.0%
!!! AAGAAGAGGAGGAGGCGTTT+TGG + chr7.4:7684828-7684847 MS.gene051757:CDS 50.0%
! CCGGTGCTTTTCTCGGTGAA+CGG + chr7.4:7684908-7684927 MS.gene051757:CDS 55.0%
! CCGTTCACCGAGAAAAGCAC+CGG - chr7.4:7684911-7684930 None:intergenic 55.0%
! CGGTGCTTTTCTCGGTGAAC+GGG + chr7.4:7684909-7684928 MS.gene051757:CDS 55.0%
! GTCATCGCCGGTGCTTTTCT+CGG + chr7.4:7684901-7684920 MS.gene051757:CDS 55.0%
!! AAAAGCACCGGCGATGACGA+AGG - chr7.4:7684899-7684918 None:intergenic 55.0%
!!! AGAGGAGGAGGCGTTTTGGA+AGG + chr7.4:7684832-7684851 MS.gene051757:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 7684811 7685060 7684811 ID=MS.gene051757
chr7.4 mRNA 7684811 7685060 7684811 ID=MS.gene051757.t1;Parent=MS.gene051757
chr7.4 exon 7684811 7684930 7684811 ID=MS.gene051757.t1.exon1;Parent=MS.gene051757.t1
chr7.4 CDS 7684811 7684930 7684811 ID=cds.MS.gene051757.t1;Parent=MS.gene051757.t1
chr7.4 exon 7685013 7685060 7685013 ID=MS.gene051757.t1.exon2;Parent=MS.gene051757.t1
chr7.4 CDS 7685013 7685060 7685013 ID=cds.MS.gene051757.t1;Parent=MS.gene051757.t1
Gene Sequence

>MS.gene051757

ATGGAATCCGCTGCTAAAAGAAGAGGAGGAGGCGTTTTGGAAGGCCTTTACAAGGTTCTTATGCGCCGCAACTCTGTCTATGTTACCTTCGTCATCGCCGGTGCTTTTCTCGGTGAACGGGTTAGCTAATTTTCCCCCTTTTAATTTTACTCTAATCGTCTATTCTTATTTGCTTATCGAAGATCGCTGAATTTCGTTGCAGGCTGTGGATTACGGTGTTCACAAGCTCTGGGAAATAAATAATGTTGGG

Protein sequence

>MS.gene051757.t1

MESAAKRRGGGVLEGLYKVLMRRNSVYVTFVIAGAFLGERAVDYGVHKLWEINNVG