Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51809.t1 | XP_013459052.1 | 98.7 | 156 | 2 | 0 | 1 | 156 | 1 | 156 | 1.10E-77 | 299.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51809.t1 | Q9S7P9 | 52.1 | 163 | 68 | 3 | 1 | 156 | 1 | 160 | 1.1e-30 | 134.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51809.t1 | A0A072V4J0 | 98.7 | 156 | 2 | 0 | 1 | 156 | 1 | 156 | 8.0e-78 | 299.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057925 | MS.gene51809 | 0.821249 | 4.46E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51809.t1 | MTR_3g023720 | 98.718 | 156 | 2 | 0 | 1 | 156 | 1 | 156 | 6.94e-113 | 321 |
| MS.gene51809.t1 | MTR_3g023720 | 98.718 | 156 | 2 | 0 | 1 | 156 | 1 | 156 | 9.02e-113 | 322 |
| MS.gene51809.t1 | MTR_4g109310 | 62.733 | 161 | 54 | 3 | 1 | 156 | 1 | 160 | 1.09e-52 | 169 |
| MS.gene51809.t1 | MTR_8g021950 | 68.000 | 75 | 24 | 0 | 82 | 156 | 13 | 87 | 1.48e-29 | 108 |
| MS.gene51809.t1 | MTR_5g043850 | 62.857 | 70 | 26 | 0 | 87 | 156 | 62 | 131 | 5.53e-25 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51809.t1 | AT5G61210 | 52.381 | 168 | 60 | 6 | 1 | 156 | 1 | 160 | 2.16e-41 | 140 |
| MS.gene51809.t1 | AT5G61210 | 52.381 | 168 | 60 | 6 | 1 | 156 | 1 | 160 | 2.16e-41 | 140 |
| MS.gene51809.t1 | AT1G13890 | 62.791 | 86 | 32 | 0 | 71 | 156 | 40 | 125 | 8.47e-34 | 120 |
| MS.gene51809.t1 | AT1G13890 | 62.791 | 86 | 32 | 0 | 71 | 156 | 40 | 125 | 8.47e-34 | 120 |
| MS.gene51809.t1 | AT5G07880 | 43.103 | 116 | 62 | 2 | 45 | 156 | 1 | 116 | 1.42e-25 | 98.6 |
| MS.gene51809.t1 | AT5G07880 | 43.103 | 116 | 62 | 2 | 45 | 156 | 1 | 116 | 1.42e-25 | 98.6 |
Find 40 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATTCTGATGAAGAGAAAA+AGG | 0.275386 | 3.4:-16087853 | MS.gene51809:CDS |
| GGAGGAATATGCTGTTTATA+AGG | 0.310753 | 3.4:-16087625 | MS.gene51809:CDS |
| TCAATACCCTGCTTAAATTA+TGG | 0.328730 | 3.4:+16087463 | None:intergenic |
| GAGTCAATCTGTGGAGGATT+TGG | 0.329006 | 3.4:-16087646 | MS.gene51809:CDS |
| TTTGGTCATGCTGCATCATC+AGG | 0.361549 | 3.4:-16087529 | MS.gene51809:CDS |
| AGAGAGAGATAGAAAATGTT+AGG | 0.401906 | 3.4:-16087930 | None:intergenic |
| TTCTCTTCATCAGAATCAAA+GGG | 0.446731 | 3.4:+16087858 | None:intergenic |
| CGAGCATCAACATCATCAAA+AGG | 0.454347 | 3.4:+16087756 | None:intergenic |
| TTTCTCTTCATCAGAATCAA+AGG | 0.462308 | 3.4:+16087857 | None:intergenic |
| TATTAGTTTCTCTCTGAACT+AGG | 0.463530 | 3.4:+16087784 | None:intergenic |
| GGCTGAAGAGACGACAAAAT+CGG | 0.495076 | 3.4:-16087604 | MS.gene51809:CDS |
| GCATTGACCATAATTTAAGC+AGG | 0.500853 | 3.4:-16087470 | MS.gene51809:CDS |
| TCATCTTATGCCCCGTCCTA+CGG | 0.516744 | 3.4:-16087717 | MS.gene51809:CDS |
| AGAGGATGCTACCAAGACTT+TGG | 0.532804 | 3.4:-16087547 | MS.gene51809:CDS |
| CAAAATCGGTGAACAATTGT+CGG | 0.533276 | 3.4:-16087590 | MS.gene51809:CDS |
| TCAATCTGTGGAGGATTTGG+AGG | 0.539441 | 3.4:-16087643 | MS.gene51809:CDS |
| AAGAACGACTTCCGTGACTC+TGG | 0.543178 | 3.4:-16087678 | MS.gene51809:CDS |
| CTGGGTTTAGCATCTTTCAA+AGG | 0.544377 | 3.4:+16087894 | None:intergenic |
| ATGCAGCATGACCAAAGTCT+TGG | 0.547106 | 3.4:+16087536 | None:intergenic |
| ATCAACATCATCAAAAGGGT+TGG | 0.547359 | 3.4:+16087761 | None:intergenic |
| TGTTTCGATTTCCGTAGGAC+GGG | 0.548263 | 3.4:+16087706 | None:intergenic |
| AAGGGATTATGCTTAGACCT+GGG | 0.549583 | 3.4:+16087876 | None:intergenic |
| AAAGGGATTATGCTTAGACC+TGG | 0.553655 | 3.4:+16087875 | None:intergenic |
| ATCAGGGCGAGCAGATTACA+AGG | 0.553800 | 3.4:-16087512 | MS.gene51809:CDS |
| GTTTCGATTTCCGTAGGACG+GGG | 0.567133 | 3.4:+16087707 | None:intergenic |
| AGGAGTCACGATATTGCTGC+TGG | 0.568758 | 3.4:-16087492 | MS.gene51809:CDS |
| CTGAACTAGGTTATATTCAG+AGG | 0.568978 | 3.4:+16087797 | None:intergenic |
| ATGTTTCGATTTCCGTAGGA+CGG | 0.580913 | 3.4:+16087705 | None:intergenic |
| ATACATGTTTCGATTTCCGT+AGG | 0.590555 | 3.4:+16087701 | None:intergenic |
| GAGCATCAACATCATCAAAA+GGG | 0.590617 | 3.4:+16087757 | None:intergenic |
| TTTGATGATGTTGATGCTCG+TGG | 0.590946 | 3.4:-16087753 | MS.gene51809:CDS |
| AACGACTTCCGTGACTCTGG+TGG | 0.615758 | 3.4:-16087675 | MS.gene51809:CDS |
| CATTGACCATAATTTAAGCA+GGG | 0.624462 | 3.4:-16087469 | MS.gene51809:CDS |
| TCTGCAATCCACCAGAGTCA+CGG | 0.629953 | 3.4:+16087667 | None:intergenic |
| TTGGTCATGCTGCATCATCA+GGG | 0.636187 | 3.4:-16087528 | MS.gene51809:CDS |
| GATAGCAGAGGAAATGCGAG+AGG | 0.657516 | 3.4:-16087565 | MS.gene51809:CDS |
| ATTGCAGAGTCAATCTGTGG+AGG | 0.684326 | 3.4:-16087652 | MS.gene51809:CDS |
| TGGATTGCAGAGTCAATCTG+TGG | 0.696583 | 3.4:-16087655 | MS.gene51809:CDS |
| CTTTGAAAGATGCTAAACCC+AGG | 0.703733 | 3.4:-16087893 | MS.gene51809:CDS |
| CAATTGTCGGAAGATAGCAG+AGG | 0.756558 | 3.4:-16087577 | MS.gene51809:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CATTGACCATAATTTAAGCA+GGG | - | chr3.4:16087889-16087908 | MS.gene51809:CDS | 30.0% | |
| TCAATACCCTGCTTAAATTA+TGG | + | chr3.4:16087898-16087917 | None:intergenic | 30.0% | |
| TGATTCTGATGAAGAGAAAA+AGG | - | chr3.4:16087505-16087524 | MS.gene51809:CDS | 30.0% | |
| TTCTCTTCATCAGAATCAAA+GGG | + | chr3.4:16087503-16087522 | None:intergenic | 30.0% | |
| TTTCTCTTCATCAGAATCAA+AGG | + | chr3.4:16087504-16087523 | None:intergenic | 30.0% | |
| ! | TATTAGTTTCTCTCTGAACT+AGG | + | chr3.4:16087577-16087596 | None:intergenic | 30.0% |
| ATACATGTTTCGATTTCCGT+AGG | + | chr3.4:16087660-16087679 | None:intergenic | 35.0% | |
| ATCAACATCATCAAAAGGGT+TGG | + | chr3.4:16087600-16087619 | None:intergenic | 35.0% | |
| CAAAATCGGTGAACAATTGT+CGG | - | chr3.4:16087768-16087787 | MS.gene51809:CDS | 35.0% | |
| CTGAACTAGGTTATATTCAG+AGG | + | chr3.4:16087564-16087583 | None:intergenic | 35.0% | |
| GAGCATCAACATCATCAAAA+GGG | + | chr3.4:16087604-16087623 | None:intergenic | 35.0% | |
| ! | GCATTGACCATAATTTAAGC+AGG | - | chr3.4:16087888-16087907 | MS.gene51809:CDS | 35.0% |
| ! | GGAGGAATATGCTGTTTATA+AGG | - | chr3.4:16087733-16087752 | MS.gene51809:CDS | 35.0% |
| AAAGGGATTATGCTTAGACC+TGG | + | chr3.4:16087486-16087505 | None:intergenic | 40.0% | |
| AAGGGATTATGCTTAGACCT+GGG | + | chr3.4:16087485-16087504 | None:intergenic | 40.0% | |
| ATGTTTCGATTTCCGTAGGA+CGG | + | chr3.4:16087656-16087675 | None:intergenic | 40.0% | |
| CGAGCATCAACATCATCAAA+AGG | + | chr3.4:16087605-16087624 | None:intergenic | 40.0% | |
| CTGGGTTTAGCATCTTTCAA+AGG | + | chr3.4:16087467-16087486 | None:intergenic | 40.0% | |
| ! | CTTTGAAAGATGCTAAACCC+AGG | - | chr3.4:16087465-16087484 | MS.gene51809:CDS | 40.0% |
| !! | TTTGATGATGTTGATGCTCG+TGG | - | chr3.4:16087605-16087624 | MS.gene51809:CDS | 40.0% |
| AGAGGATGCTACCAAGACTT+TGG | - | chr3.4:16087811-16087830 | MS.gene51809:CDS | 45.0% | |
| ATGCAGCATGACCAAAGTCT+TGG | + | chr3.4:16087825-16087844 | None:intergenic | 45.0% | |
| ATTGCAGAGTCAATCTGTGG+AGG | - | chr3.4:16087706-16087725 | MS.gene51809:CDS | 45.0% | |
| CAATTGTCGGAAGATAGCAG+AGG | - | chr3.4:16087781-16087800 | MS.gene51809:CDS | 45.0% | |
| GAGTCAATCTGTGGAGGATT+TGG | - | chr3.4:16087712-16087731 | MS.gene51809:CDS | 45.0% | |
| GGCTGAAGAGACGACAAAAT+CGG | - | chr3.4:16087754-16087773 | MS.gene51809:CDS | 45.0% | |
| TCAATCTGTGGAGGATTTGG+AGG | - | chr3.4:16087715-16087734 | MS.gene51809:CDS | 45.0% | |
| TGGATTGCAGAGTCAATCTG+TGG | - | chr3.4:16087703-16087722 | MS.gene51809:CDS | 45.0% | |
| TGTTTCGATTTCCGTAGGAC+GGG | + | chr3.4:16087655-16087674 | None:intergenic | 45.0% | |
| TTGGTCATGCTGCATCATCA+GGG | - | chr3.4:16087830-16087849 | MS.gene51809:CDS | 45.0% | |
| TTTGGTCATGCTGCATCATC+AGG | - | chr3.4:16087829-16087848 | MS.gene51809:CDS | 45.0% | |
| AAGAACGACTTCCGTGACTC+TGG | - | chr3.4:16087680-16087699 | MS.gene51809:CDS | 50.0% | |
| AGGAGTCACGATATTGCTGC+TGG | - | chr3.4:16087866-16087885 | MS.gene51809:CDS | 50.0% | |
| ATCAGGGCGAGCAGATTACA+AGG | - | chr3.4:16087846-16087865 | MS.gene51809:CDS | 50.0% | |
| GATAGCAGAGGAAATGCGAG+AGG | - | chr3.4:16087793-16087812 | MS.gene51809:CDS | 50.0% | |
| GTTTCGATTTCCGTAGGACG+GGG | + | chr3.4:16087654-16087673 | None:intergenic | 50.0% | |
| TCATCTTATGCCCCGTCCTA+CGG | - | chr3.4:16087641-16087660 | MS.gene51809:CDS | 50.0% | |
| TCTGCAATCCACCAGAGTCA+CGG | + | chr3.4:16087694-16087713 | None:intergenic | 50.0% | |
| AACGACTTCCGTGACTCTGG+TGG | - | chr3.4:16087683-16087702 | MS.gene51809:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 16087443 | 16087937 | 16087443 | ID=MS.gene51809 |
| chr3.4 | mRNA | 16087443 | 16087937 | 16087443 | ID=MS.gene51809.t1;Parent=MS.gene51809 |
| chr3.4 | exon | 16087443 | 16087937 | 16087443 | ID=MS.gene51809.t1.exon1;Parent=MS.gene51809.t1 |
| chr3.4 | CDS | 16087443 | 16087937 | 16087443 | ID=cds.MS.gene51809.t1;Parent=MS.gene51809.t1 |
| Gene Sequence |
| Protein sequence |
