Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51846.t1 | AES80205.1 | 90.7 | 86 | 8 | 0 | 1 | 86 | 107 | 192 | 1.40E-34 | 155.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51846.t1 | A0A3Q7JC00 | 67.5 | 80 | 25 | 1 | 1 | 80 | 573 | 651 | 1.5e-18 | 93.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51846.t1 | G7L4Q7 | 90.7 | 86 | 8 | 0 | 1 | 86 | 107 | 192 | 9.7e-35 | 155.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene51846 | 0.838106 | 3.50E-57 | -1.69E-46 |
| MS.gene05415 | MS.gene51846 | 0.801078 | 1.07E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTCAGACTTGCCGCAAAC+TGG | 0.407898 | 7.4:+28308148 | None:intergenic |
| TCACCTGAAGGAAAAGAAAC+TGG | 0.448899 | 7.4:+28308454 | MS.gene51846:CDS |
| AATAGTTCGGCTTATATCTC+AGG | 0.451231 | 7.4:+28308185 | MS.gene51846:CDS |
| AGCATCCCAATCAACTGGTT+GGG | 0.452727 | 7.4:-28308897 | None:intergenic |
| CTTCTGATATGCAAATAGTT+CGG | 0.480930 | 7.4:+28308172 | MS.gene51846:CDS |
| GTAAAAGCATCCCAATCAAC+TGG | 0.488559 | 7.4:-28308902 | None:intergenic |
| CATATCAGAAGGCCAGTTTG+CGG | 0.519982 | 7.4:-28308160 | None:intergenic |
| GACTAGGCAACATGTAAGAA+AGG | 0.521743 | 7.4:+28308385 | MS.gene51846:intron |
| AAGCATCCCAATCAACTGGT+TGG | 0.530896 | 7.4:-28308898 | None:intergenic |
| AATAGTAGCAATGTCTGTGA+TGG | 0.562084 | 7.4:-28308861 | None:intergenic |
| GGTGACTATGAGATCGACTT+GGG | 0.566951 | 7.4:-28308436 | None:intergenic |
| AGGTGACTATGAGATCGACT+TGG | 0.573986 | 7.4:-28308437 | None:intergenic |
| TCGATCTCATAGTCACCTGA+AGG | 0.609058 | 7.4:+28308442 | MS.gene51846:CDS |
| CGAACTATTTGCATATCAGA+AGG | 0.611737 | 7.4:-28308171 | None:intergenic |
| CAACGGCTATATGTTACCTG+AGG | 0.696116 | 7.4:-28308924 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATATTATTTAATTTTA+GGG | + | chr7.4:28308822-28308841 | MS.gene51846:intron | 0.0% |
| !!! | TAAATATATTATTTAATTTT+AGG | + | chr7.4:28308821-28308840 | MS.gene51846:intron | 0.0% |
| !! | ATATATTACAAATTGAATTA+AGG | - | chr7.4:28308291-28308310 | None:intergenic | 10.0% |
| !! | TAATAAAAAAAACAAAATTG+AGG | - | chr7.4:28308236-28308255 | None:intergenic | 10.0% |
| !!! | CTAGAATTAATTTTTTTTAA+TGG | + | chr7.4:28308671-28308690 | MS.gene51846:intron | 10.0% |
| !! | TAATATATATTGATGTCTTT+AGG | + | chr7.4:28308311-28308330 | MS.gene51846:intron | 15.0% |
| !!! | TTTGTTTTTTTTATTACATG+AGG | + | chr7.4:28308240-28308259 | MS.gene51846:intron | 15.0% |
| !! | TACAATATTAATTTCATGAC+CGG | + | chr7.4:28308701-28308720 | MS.gene51846:intron | 20.0% |
| !! | TGGAATTAATAATATTCTAC+CGG | - | chr7.4:28308723-28308742 | None:intergenic | 20.0% |
| ! | ACACCTCAATTTCATTATTT+AGG | - | chr7.4:28308578-28308597 | None:intergenic | 25.0% |
| ! | ACCTCAATTTCATTATTTAG+GGG | - | chr7.4:28308576-28308595 | None:intergenic | 25.0% |
| ! | ATTAATGATTCAGAACCAAA+AGG | - | chr7.4:28308635-28308654 | None:intergenic | 25.0% |
| ! | CACATCTACAAAAAAAATCT+AGG | - | chr7.4:28308348-28308367 | None:intergenic | 25.0% |
| ! | CACCTCAATTTCATTATTTA+GGG | - | chr7.4:28308577-28308596 | None:intergenic | 25.0% |
| ! | TTGGGTTTAAATTAGAAACT+CGG | - | chr7.4:28308490-28308509 | None:intergenic | 25.0% |
| !! | TAATTTTGTATGTTGTGACT+AGG | + | chr7.4:28308369-28308388 | MS.gene51846:intron | 25.0% |
| CTTCTGATATGCAAATAGTT+CGG | + | chr7.4:28308172-28308191 | MS.gene51846:CDS | 30.0% | |
| TATTTAGGGGAAGAATATCA+GGG | - | chr7.4:28308563-28308582 | None:intergenic | 30.0% | |
| TCCCCTAAATAATGAAATTG+AGG | + | chr7.4:28308572-28308591 | MS.gene51846:intron | 30.0% | |
| TTATTTAGGGGAAGAATATC+AGG | - | chr7.4:28308564-28308583 | None:intergenic | 30.0% | |
| TTCTAACTAAGAAAACAACC+CGG | - | chr7.4:28308525-28308544 | None:intergenic | 30.0% | |
| !! | AACCCAATTGTTTTACATTC+CGG | + | chr7.4:28308503-28308522 | MS.gene51846:intron | 30.0% |
| AAAAACCCAACCAGTTGATT+GGG | + | chr7.4:28308892-28308911 | MS.gene51846:CDS | 35.0% | |
| AACCCGGAATGTAAAACAAT+TGG | - | chr7.4:28308509-28308528 | None:intergenic | 35.0% | |
| AATAGTAGCAATGTCTGTGA+TGG | - | chr7.4:28308864-28308883 | None:intergenic | 35.0% | |
| AATAGTTCGGCTTATATCTC+AGG | + | chr7.4:28308185-28308204 | MS.gene51846:CDS | 35.0% | |
| ACCCGGAATGTAAAACAATT+GGG | - | chr7.4:28308508-28308527 | None:intergenic | 35.0% | |
| CGAACTATTTGCATATCAGA+AGG | - | chr7.4:28308174-28308193 | None:intergenic | 35.0% | |
| ! | CTCAAGAACTCAATTCCTTT+TGG | + | chr7.4:28308617-28308636 | MS.gene51846:intron | 35.0% |
| !! | ACCCAATTGTTTTACATTCC+GGG | + | chr7.4:28308504-28308523 | MS.gene51846:intron | 35.0% |
| !! | AGGAAGATCTCCTAGTTTTT+CGG | + | chr7.4:28308405-28308424 | MS.gene51846:CDS | 35.0% |
| !! | GGTGTTATTTCTGAGTATCA+TGG | - | chr7.4:28308743-28308762 | None:intergenic | 35.0% |
| AAATTGAGGTGTGATACTGC+GGG | + | chr7.4:28308586-28308605 | MS.gene51846:intron | 40.0% | |
| CTGAAGGAAAAGAAACTGGT+AGG | + | chr7.4:28308458-28308477 | MS.gene51846:intron | 40.0% | |
| CTGTGTAGACAAGTAACACT+TGG | - | chr7.4:28308787-28308806 | None:intergenic | 40.0% | |
| GAAAAACCCAACCAGTTGAT+TGG | + | chr7.4:28308891-28308910 | MS.gene51846:CDS | 40.0% | |
| GAAATTGAGGTGTGATACTG+CGG | + | chr7.4:28308585-28308604 | MS.gene51846:intron | 40.0% | |
| GACTAGGCAACATGTAAGAA+AGG | + | chr7.4:28308385-28308404 | MS.gene51846:intron | 40.0% | |
| TCACCTGAAGGAAAAGAAAC+TGG | + | chr7.4:28308454-28308473 | MS.gene51846:CDS | 40.0% | |
| TTGTCTACACAGTATTGCTG+TGG | + | chr7.4:28308795-28308814 | MS.gene51846:intron | 40.0% | |
| ! | CTACCAGTTTCTTTTCCTTC+AGG | - | chr7.4:28308460-28308479 | None:intergenic | 40.0% |
| !! | GGAAGATCTCCTAGTTTTTC+GGG | + | chr7.4:28308406-28308425 | MS.gene51846:CDS | 40.0% |
| !! | GTAAAAGCATCCCAATCAAC+TGG | - | chr7.4:28308905-28308924 | None:intergenic | 40.0% |
| AAGCATCCCAATCAACTGGT+TGG | - | chr7.4:28308901-28308920 | None:intergenic | 45.0% | |
| AGCATCCCAATCAACTGGTT+GGG | - | chr7.4:28308900-28308919 | None:intergenic | 45.0% | |
| AGGTGACTATGAGATCGACT+TGG | - | chr7.4:28308440-28308459 | None:intergenic | 45.0% | |
| AGTAACACTTGGCACCAAAG+AGG | - | chr7.4:28308776-28308795 | None:intergenic | 45.0% | |
| CAACGGCTATATGTTACCTG+AGG | - | chr7.4:28308927-28308946 | None:intergenic | 45.0% | |
| CACCAAAGAGGGTAACTCTA+CGG | - | chr7.4:28308764-28308783 | None:intergenic | 45.0% | |
| CATATCAGAAGGCCAGTTTG+CGG | - | chr7.4:28308163-28308182 | None:intergenic | 45.0% | |
| GTAACACTTGGCACCAAAGA+GGG | - | chr7.4:28308775-28308794 | None:intergenic | 45.0% | |
| TCGATCTCATAGTCACCTGA+AGG | + | chr7.4:28308442-28308461 | MS.gene51846:CDS | 45.0% | |
| ! | GATTGGGATGCTTTTACCTC+AGG | + | chr7.4:28308908-28308927 | MS.gene51846:CDS | 45.0% |
| ! | GGTGACTATGAGATCGACTT+GGG | - | chr7.4:28308439-28308458 | None:intergenic | 45.0% |
| CACCGTAGAGTTACCCTCTT+TGG | + | chr7.4:28308759-28308778 | MS.gene51846:intron | 50.0% | |
| GGTTCACTGCCCGAAAAACT+AGG | - | chr7.4:28308418-28308437 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 28308155 | 28308947 | 28308155 | ID=MS.gene51846 |
| chr7.4 | mRNA | 28308155 | 28308947 | 28308155 | ID=MS.gene51846.t1;Parent=MS.gene51846 |
| chr7.4 | exon | 28308155 | 28308226 | 28308155 | ID=MS.gene51846.t1.exon1;Parent=MS.gene51846.t1 |
| chr7.4 | CDS | 28308155 | 28308226 | 28308155 | ID=cds.MS.gene51846.t1;Parent=MS.gene51846.t1 |
| chr7.4 | exon | 28308391 | 28308475 | 28308391 | ID=MS.gene51846.t1.exon2;Parent=MS.gene51846.t1 |
| chr7.4 | CDS | 28308391 | 28308475 | 28308391 | ID=cds.MS.gene51846.t1;Parent=MS.gene51846.t1 |
| chr7.4 | exon | 28308843 | 28308947 | 28308843 | ID=MS.gene51846.t1.exon3;Parent=MS.gene51846.t1 |
| chr7.4 | CDS | 28308843 | 28308947 | 28308843 | ID=cds.MS.gene51846.t1;Parent=MS.gene51846.t1 |
| Gene Sequence |
| Protein sequence |
